P W Kuchel

Summary

Affiliation: University of Sydney
Country: Australia

Publications

  1. ncbi request reprint Metabolic homeostasis in the human erythrocyte: in silico analysis
    Pedro de Atauri
    Unitat de Bioquimica, Departament de Ciències Fisiològiques I, Facultat de Medicina, Institut d Investigacions Biomediques August Pi i Sunyer IDIBAPS, Universitat de Barcelona, Barcelona, Spain
    Biosystems 83:118-24. 2006
  2. doi request reprint 2H2O quadrupolar splitting used to measure water exchange in erythrocytes
    Philip W Kuchel
    School of Molecular and Microbial Biosciences, University of Sydney, Building G08, NSW 2006, Australia
    J Magn Reson 192:48-59. 2008
  3. ncbi request reprint Isotopomer subspaces as indicators of metabolic-pathway structure
    Philip W Kuchel
    School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia
    J Theor Biol 252:391-401. 2008
  4. ncbi request reprint Current status and challenges in connecting models of erythrocyte metabolism to experimental reality
    Philip W Kuchel
    School of Molecular and Microbial Biosciences, University of Sydney, Building G08, Sydney, NSW 2006, Australia
    Prog Biophys Mol Biol 85:325-42. 2004
  5. ncbi request reprint Why does the mammalian red blood cell have aquaporins?
    Philip W Kuchel
    School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia
    Biosystems 82:189-96. 2005
  6. ncbi request reprint 31P MAS-NMR of human erythrocytes: independence of cell volume from angular velocity
    P W Kuchel
    School of Molecular and Microbial Biosciences, University of Sydney, NSW, Australia
    Magn Reson Med 52:663-8. 2004
  7. ncbi request reprint Measurement of compartment size in q-space experiments: Fourier transform of the second derivative
    Philip W Kuchel
    School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia
    Magn Reson Med 52:907-12. 2004
  8. ncbi request reprint The story of the discovery of aquaporins: convergent evolution of ideas--but who got there first?
    P W Kuchel
    School of Molecular and Microbial Biosciences, University of Sydney, NSW, Australia
    Cell Mol Biol (Noisy-le-grand) 52:2-5. 2006
  9. ncbi request reprint Apparatus for rapid adjustment of the degree of alignment of NMR samples in aqueous media: verification with residual quadrupolar splittings in (23)Na and (133)Cs spectra
    Philip W Kuchel
    School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia
    J Magn Reson 180:256-65. 2006
  10. ncbi request reprint NMR studies of erythrocytes immobilized in agarose and alginate gels
    P Lundberg
    Department of Biochemistry, University of Sydney, New South Wales, Australia
    Magn Reson Med 25:273-88. 1992

Collaborators

Detail Information

Publications79

  1. ncbi request reprint Metabolic homeostasis in the human erythrocyte: in silico analysis
    Pedro de Atauri
    Unitat de Bioquimica, Departament de Ciències Fisiològiques I, Facultat de Medicina, Institut d Investigacions Biomediques August Pi i Sunyer IDIBAPS, Universitat de Barcelona, Barcelona, Spain
    Biosystems 83:118-24. 2006
    ....
  2. doi request reprint 2H2O quadrupolar splitting used to measure water exchange in erythrocytes
    Philip W Kuchel
    School of Molecular and Microbial Biosciences, University of Sydney, Building G08, NSW 2006, Australia
    J Magn Reson 192:48-59. 2008
    ....
  3. ncbi request reprint Isotopomer subspaces as indicators of metabolic-pathway structure
    Philip W Kuchel
    School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia
    J Theor Biol 252:391-401. 2008
    ..As part of this discussion we introduce the term 'combinatorial deficit' of all possible isotopomers and we show that this deficit is less for the F- than the L-type pathway...
  4. ncbi request reprint Current status and challenges in connecting models of erythrocyte metabolism to experimental reality
    Philip W Kuchel
    School of Molecular and Microbial Biosciences, University of Sydney, Building G08, Sydney, NSW 2006, Australia
    Prog Biophys Mol Biol 85:325-42. 2004
    ..These matters are discussed in the light of new modelling strategies...
  5. ncbi request reprint Why does the mammalian red blood cell have aquaporins?
    Philip W Kuchel
    School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia
    Biosystems 82:189-96. 2005
    ..The ideas are generalizable to other cell types and organelles...
  6. ncbi request reprint 31P MAS-NMR of human erythrocytes: independence of cell volume from angular velocity
    P W Kuchel
    School of Molecular and Microbial Biosciences, University of Sydney, NSW, Australia
    Magn Reson Med 52:663-8. 2004
    ..The relationship of these conclusions to other non-NMR studies of pressure effects on cells is noted...
  7. ncbi request reprint Measurement of compartment size in q-space experiments: Fourier transform of the second derivative
    Philip W Kuchel
    School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia
    Magn Reson Med 52:907-12. 2004
    ..It should prove particularly useful where the coherence features are poorly defined or where more than one pattern is present in a q-space plot...
  8. ncbi request reprint The story of the discovery of aquaporins: convergent evolution of ideas--but who got there first?
    P W Kuchel
    School of Molecular and Microbial Biosciences, University of Sydney, NSW, Australia
    Cell Mol Biol (Noisy-le-grand) 52:2-5. 2006
    ....
  9. ncbi request reprint Apparatus for rapid adjustment of the degree of alignment of NMR samples in aqueous media: verification with residual quadrupolar splittings in (23)Na and (133)Cs spectra
    Philip W Kuchel
    School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia
    J Magn Reson 180:256-65. 2006
    ..It should be useful with these and other cell types, for studies of metabolic and membrane transport characteristics that may change when the cells are distorted, and possibly for structural studies of macromolecules...
  10. ncbi request reprint NMR studies of erythrocytes immobilized in agarose and alginate gels
    P Lundberg
    Department of Biochemistry, University of Sydney, New South Wales, Australia
    Magn Reson Med 25:273-88. 1992
    ..In summary, immobilization in Ba-alginate-coated agarose threads was an efficient way of trapping human erythrocytes for whole cell NMR investigations...
  11. ncbi request reprint Modelling Staphylococcus aureus-induced septicemia using NMR
    R Plummer
    School of Molecular and Microbial Biosciences, University of Sydney, Sydney, Australia
    Magn Reson Med 58:656-65. 2007
    ..The results are encouraging for the use of NMR spectroscopy in clinical microbiology...
  12. pmc Hemoglobin affinity for 2,3-bisphosphoglycerate in solutions and intact erythrocytes: studies using pulsed-field gradient nuclear magnetic resonance and Monte Carlo simulations
    A J Lennon
    Department of Biochemistry, University of Sydney, New South Wales, Australia
    Biophys J 67:2096-109. 1994
    ..In addition, the Monte Carlo simulations of the diffusion experiments highlighted important differences between theory and experiment relating to the nature of molecular motion inside the cells...
  13. doi request reprint Quantitative model of NMR chemical shifts of 23Na+ induced by TmDOTP: applications in studies of Na+ transport in human erythrocytes
    M Puckeridge
    School of Molecular Bioscience, G08, University of Sydney, New South Wales 2006, Australia
    J Inorg Biochem 115:211-9. 2012
    ..In addition to the model, parameter fitting was achieved by calculating the likelihood distribution of parameters, and seeking the maximum likelihood with a Bayesian type of analysis...
  14. pmc Identification of extracellular phospholipase B, lysophospholipase, and acyltransferase produced by Cryptococcus neoformans
    S C Chen
    Centre for Infectious Diseases and Microbiology, University of Sydney, New South Wales, Australia
    Infect Immun 65:405-11. 1997
    ..5 to 4.5. Activities were unchanged in the presence of exogenous serine protease inhibitors, divalent cations, and EDTA. We conclude that C. neoformans produces highly active extracellular PLB, LPL, and LPTA under native conditions...
  15. ncbi request reprint Changes in cellular and plasma membrane phospholipid composition after lipopolysaccharide stimulation of human neutrophils, studied by 31P NMR
    L C Wright
    Centre for Infectious Diseases and Microbiology, University of Sydney, Australia
    Eur J Biochem 243:328-35. 1997
    ..This report is the first to elaborate the changes in phospholipid composition in human neutrophils as a whole, and in plasma membranes separated from them, before and after stimulation by the physiological activator, LPS...
  16. pmc Model of 2,3-bisphosphoglycerate metabolism in the human erythrocyte based on detailed enzyme kinetic equations: in vivo kinetic characterization of 2,3-bisphosphoglycerate synthase/phosphatase using 13C and 31P NMR
    P J Mulquiney
    Department of Biochemistry, University of Sydney, Sydney, NSW 2006, Australia
    Biochem J 342:567-80. 1999
    ..Using the newly determined kinetic parameters, the percentage of glycolytic carbon flux that passes through the 2, 3-BPG shunt in the normal in vivo steady state was estimated to be 19%...
  17. pmc Simulations of molecular diffusion in lattices of cells: insights for NMR of red blood cells
    David G Regan
    School of Molecular and Microbial Biosciences, University of Sydney, New South Wales 2006, Australia
    Biophys J 83:161-71. 2002
    ..The simulated data were compared with those from real PGSE NMR diffusion experiments on RBC suspensions that had a range of hematocrit values. Methods that facilitate the processing of q-space data were also developed...
  18. doi request reprint Erythrocyte orientational and cell volume effects on NMR q-space analysis: simulations of restricted diffusion
    Timothy J Larkin
    School of Molecular and Microbial Biosciences, University of Sydney, Building G08, Sydney, NSW, 2006, Australia
    Eur Biophys J 39:139-48. 2009
    ..We show that these changes are reflected in the appearance of the plots in a way that is diagnostic of the microscopic geometry of the sample...
  19. ncbi request reprint Structure of the HERG K+ channel S5P extracellular linker: role of an amphipathic alpha-helix in C-type inactivation
    Allan M Torres
    School of Molecular and Microbial Biosciences, University of Sydney, New South Wales 2006, Australia
    J Biol Chem 278:42136-48. 2003
    ..Electrophysiological studies of HERG currents following application of exogenous S5P peptides show that the amphipathic helix in the S5P linker interacts with the pore region of the channel in a voltage-dependent manner...
  20. doi request reprint Human erythrocyte flickering: temperature, ATP concentration, water transport, and cell aging, plus a computer simulation
    David Szekely
    School of Molecular and Microbial Biosciences, University of Sydney, Building G08, Sydney, NSW, 2006, Australia
    Eur Biophys J 38:923-39. 2009
    ....
  21. doi request reprint Water chemical shift in 1H NMR of red cells: effects of pH when transmembrane magnetic susceptibility differences are low
    Timothy J Larkin
    School of Molecular and Microbial Biosciences, University of Sydney, Australia
    Magn Reson Med 59:707-11. 2008
    ..The findings are fundamental to an interpretation of the mechanism of chemical shift effects on the water resonance that may occur in functional MRI...
  22. ncbi request reprint Interactions of Na+ with haemoglobin-organic phosphate complexes
    G L Mendz
    Department of Biochemistry, University of Sydney, N S W, Australia
    Biophys Chem 30:81-92. 1988
    ..Results obtained with adenosine triphosphate and D-glucose indicate that there is also interaction between Na+ and haemoglobin complexes of these molecules...
  23. ncbi request reprint Fructose 3-phosphate and 5-phosphoribosyl-1-pyrophosphate formation in perfused human erythrocytes: 31P NMR studies
    P Lundberg
    Department of Biochemistry, University of Sydney, Australia
    Magn Reson Med 31:110-21. 1994
    ..C. 2.7.6.1) activity in the human erythrocytes and that the biosynthetic pathways are active in immobilized cells at 37 degrees C. (5) The rates of accumulation of 2,3-DPG and phosphomonoesters (PME) appeared to be strongly correlated...
  24. ncbi request reprint gamma-Glutamylcyclotransferase: inhibition by D-beta-aminoglutaryl-L-alanine and analysis of the solvent kinetic isotope effect
    M J York
    Department of Biochemistry, University of Sydney, Australia
    Eur J Biochem 184:97-101. 1989
    ..We propose that the solvent kinetic isotope effect is due to an intramolecular proton transfer between the glutamyl amino group and the peptide bond nitrogen...
  25. ncbi request reprint The beta-defensin-fold family of polypeptides
    Allan M Torres
    School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia
    Toxicon 44:581-8. 2004
    ..The distinct compact fold shared by these polypeptides may be useful in the design of molecules with desired pharmacological activity...
  26. ncbi request reprint Investigation of methaemoglobin reduction by extracellular NADH in mammalian erythrocytes
    Eleanor C Kennett
    School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia
    Int J Biochem Cell Biol 37:1438-45. 2005
    ....
  27. ncbi request reprint Device for aeration and mixing of cell and organelle suspensions during NMR experiments
    Roger M Bourne
    School of Molecular and Microbial Biosciences, University of Sydney, Sydney, Australia
    J Magn Reson 159:158-60. 2002
    ..The modular design of components permits the use of the device in magnets of various internal dimensions, or with probe heads of different sample tube diameter, by modification of the simpler components of the assembly...
  28. ncbi request reprint Convection-compensating diffusion experiments with phase-sensitive double-quantum filtering
    Konstantin I Momot
    School of Molecular and Microbial Biosciences, University of Sydney, Sydney, NSW 2006, Australia
    J Magn Reson 174:229-36. 2005
    ..The proposed scheme may offer advantages in diffusion measurements of spectrally crowded systems, particularly small molecules solubilized in colloidal solutions or bound to macromolecules...
  29. ncbi request reprint Mathematical modelling of the urea cycle. A numerical investigation into substrate channelling
    Anthony D Maher
    School of Molecular and Microbial Biosciences, University of Sydney, Australia
    Eur J Biochem 270:3953-61. 2003
    ....
  30. ncbi request reprint The HERG K+ channel: progress in understanding the molecular basis of its unusual gating kinetics
    Jamie I Vandenberg
    Victor Chang Cardiac Research Institute, Level 9, 384 Victoria Street, Darlinghurst, NSW, 2010, Australia
    Eur Biophys J 33:89-97. 2004
    ..Furthermore, structural studies on scorpion toxins that potently inhibit HERG are beginning to provide clues as to the structural differences between HERG and other voltage-gated K+ channels...
  31. ncbi request reprint Acquisition of pure-phase diffusion spectra using oscillating-gradient spin echo
    Konstantin I Momot
    School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia
    J Magn Reson 176:151-9. 2005
    ..These features of OGSE can be valuable in diffusion measurements of scalar-coupled small-molecule probes in cellular and other heterogeneous systems...
  32. ncbi request reprint Solution structure of CnErg1 (Ergtoxin), a HERG specific scorpion toxin
    Allan M Torres
    School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia
    FEBS Lett 539:138-42. 2003
    ..There is also a large hydrophobic patch on the surface of the toxin, surrounding a central lysine residue, Lys13. We postulate that this hydrophobic patch is likely to form part of the binding surface of the toxin...
  33. ncbi request reprint Permeability coefficients from NMR q-space data: models with unevenly spaced semi-permeable parallel membranes
    P W Kuchel
    Department of Biochemistry, University of Sydney, NSW, 2006, Australia
    J Magn Reson 139:258-72. 1999
    ..The development of the theory was facilitated by symbolic computation, and the analysis of synthetic data was shown to be achievable by the use of a three-layer back-propagation artificial neural network...
  34. ncbi request reprint Thermodynamic and hydrodynamic properties of human tropoelastin. Analytical ultracentrifuge and pulsed field-gradient spin-echo NMR studies
    P Toonkool
    Department of Biochemistry and the Faculty of Pharmacy, University of Sydney, New South Wales 2006, Australia
    J Biol Chem 276:28042-50. 2001
    ..A critical concentration of 3.4 g/liter was obtained when the coacervate was modeled as a helical polymer formed from the monomers via oligomeric intermediates...
  35. pmc pH and cell volume effects on H2O and phosphoryl resonance splitting in rapid-spinning NMR of red cells
    Timothy J Larkin
    School of Molecular and Microbial Biosciences, University of Sydney, New South Wales 2006, Australia
    Biophys J 92:1770-6. 2007
    ..Measurements of the magnetic susceptibility difference between the two compartments were also carried out, yielding a value of 2.0 +/- 0.2 x 10(-7) (SI units) for erythrocytes in isotonic saline at pH 7.0...
  36. ncbi request reprint Chemical shift and magnetic susceptibility contributions to the separation of intracellular and supernatant resonances in variable angle spinning NMR spectra of erythrocyte suspensions
    David J Philp
    School of Molecular and Microbial Biosciences, University of Sydney, Australia
    Magn Reson Med 51:441-4. 2004
    ..Use of different angles between the spinning axis and the main magnetic field provided a method for quantifiying the isotropic chemical shift and susceptibility differences between the compartments...
  37. ncbi request reprint Enhancement of Na+ diffusion in a bicontinuous cubic phase by the ionophore monensin
    Konstantin I Momot
    School of Molecular and Microbial Biosciences, University of Sydney, Sydney, NSW 2006, Australia
    Langmuir 20:2660-6. 2004
    ..The approach could be used to study the dynamics of hydrophilic species in the aqueous channels of BCPs and similar structures, as well as to measure the ion-transporting efficiency of ionophores...
  38. ncbi request reprint Redox reactions and electron transfer across the red cell membrane
    Eleanor C Kennett
    School of Molecular and Microbial Biosciences, University of Sydney, NSW, Australia
    IUBMB Life 55:375-85. 2003
    ..We have decided to study this from a new perspective, NMR spectroscopy the area of our own technical expertise, hence this review is slanted towards this more recent analysis...
  39. ncbi request reprint Simulations of NMR-detected diffusion in suspensions of red cells: the "signatures" in q-space plots of various lattice arrangements
    David G Regan
    School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia
    Eur Biophys J 31:563-74. 2003
    ..We show that changes in the packing arrangement of cells are reflected in the q-space plots and in the results of diffusion tensor analysis and thus we speculate upon the possible clinical importance of these findings...
  40. doi request reprint Prochiral and chiral resolution in 2H NMR spectra: solutes in stretched and compressed gelatin gels
    Christoph Naumann
    School of Molecular and Microbial Biosciences, University of Sydney, New South Wales 2006, Australia
    J Phys Chem A 112:8659-64. 2008
    ..This flexibility will be important for resolving spectra of mixtures of other chiral compounds and for structure determination of selected peptides...
  41. ncbi request reprint Characterization and isolation of L-to-D-amino-acid-residue isomerase from platypus venom
    A M Torres
    School of Molecular and Microbial Biosciences, University of Sydney, Sydney, Australia
    Amino Acids 32:63-8. 2007
    ..Isolation of the isomerase was carried out by combined ion-exchange chromatography and reverse-phase high performance liquid chromatography (HPLC)...
  42. pmc Effect of caspofungin on metabolite profiles of Aspergillus species determined by nuclear magnetic resonance spectroscopy
    R Plummer
    School of Molecular and Microbial Biosciences, University of Sydney, NSW, Australia
    Antimicrob Agents Chemother 51:4077-84. 2007
    ....
  43. ncbi request reprint Heteronuclear NMR studies of metabolites produced by Cryptococcus neoformans in culture media: identification of possible virulence factors
    W A Bubb
    Department of Biochemistry, University of Sydney, NSW, Australia
    Magn Reson Med 42:442-53. 1999
    ..The possible contribution of some detected substances to the pathogenicity of Cryptococcus neoformans is discussed. Magn Reson Med 42:442-453, 1999...
  44. pmc Mechanism of Mg2+ binding in the Na+,K+-ATPase
    Anne Pilotelle-Bunner
    School of Chemistry, University of Sydney, Sydney, New South Wales, Australia
    Biophys J 96:3753-61. 2009
    ....
  45. ncbi request reprint Determination of Na+ binding parameters by relaxation analysis of selected 23Na NMR coherences: RNA, BSA and SDS
    Allan M Torres
    School of Molecular and Microbial Biosciences, University of Sydney, Sydney, New South Wales 2006, Australia
    Magn Reson Chem 43:217-24. 2005
    ....
  46. ncbi request reprint NMR studies of exchange between intra- and extracellular glutathione in human erythrocytes
    Eleanor C Kennett
    School of Molecular and Microbial Biosciences, University of Sydney, New South Wales, Australia
    Redox Rep 10:83-90. 2005
    ....
  47. ncbi request reprint Plasma membrane oxidoreductases: effects on erythrocyte metabolism and redox homeostasis
    Eleanor C Kennett
    School of Molecular and Microbial Biosciences, University of Sydney, NSW, Australia
    Antioxid Redox Signal 8:1241-7. 2006
    ....
  48. ncbi request reprint Conformations of platypus venom C-type natriuretic peptide in aqueous solution and sodium dodecyl sulfate micelles
    Allan M Torres
    Department of Biochemistry, University of Sydney, Sydney, NSW 2006, Australia
    Toxicon 40:711-9. 2002
    ..Thus, OvCNPa interacts with SDS micelles and can possibly form ion-channels in cell membranes...
  49. ncbi request reprint (39)K nuclear magnetic resonance and a mathematical model of K(+) transport in human erythrocytes
    Anthony D Maher
    School of Molecular and Microbial Biosciences, University of Sydney, 2006 Sydney, NSW, Australia
    Eur Biophys J 35:293-301. 2006
    ..The velocity of the K(+) efflux mediated by the Gárdos channel was 1.19+/-0.40 mmol (L RBC)(-1) min(-1) at 37 degrees C...
  50. doi request reprint z-Spectra of 23Na+ in stretched gels: quantitative multiple quantum analysis
    Bogdan E Chapman
    School of Molecular Bioscience, University of Sydney, Building G08, NSW 2006, Australia
    J Magn Reson 205:260-8. 2010
    ..The experimental procedures and results have implications for enhancement of contrast in (23)Na magnetic resonance imaging of heterogeneous systems using quadrupolar interactions...
  51. doi request reprint Understanding and utilising mammalian venom via a platypus venom transcriptome
    Camilla M Whittington
    Faculty of Veterinary Science, The University of Sydney, NSW 2006, Australia
    J Proteomics 72:155-64. 2009
    ..It is therefore hoped that this basic research to identify the constituents of platypus venom will eventually yield novel drugs and new targets for painkillers...
  52. ncbi request reprint Comparative study of the antioxidant defence systems in the erythrocytes of Australian marsupials and monotremes
    A T Whittington
    Department of Physiology, University of New England, Armidale, NSW, Australia
    Comp Biochem Physiol C Pharmacol Toxicol Endocrinol 110:267-72. 1995
    ..05, was observed between GSH-Px and catalase activities in the RBC of the marsupials. Since both these enzymes "detoxify" H2O2, there appears to be a reciprocal relationship between the activities of these enzymes in marsupial RBC...
  53. ncbi request reprint Experiments to detect long-range heteronuclear shift correlations: LR-J-HSMQC
    A M Torres
    School of Molecular and Microbial Biosciences, University of Sydney, Sydney, New South Wales 2006, Australia
    J Magn Reson 156:249-57. 2002
    ..LR-J-HSMQC(27,80) was very efficient in yielding two- and four-bond relay correlations. The utility of the new sequences is demonstrated with strychnine as the sample...
  54. ncbi request reprint Shear-induced alignment of self-associated hemoglobin in human erythrocytes: small angle neutron scattering studies
    C J Garvey
    Australian Nuclear Science and Technology Organisation, 2234 Menai, NSW, Australia
    Eur Biophys J 33:589-95. 2004
    ....
  55. doi request reprint Erythrocyte-shape evolution recorded with fast-measurement NMR diffusion-diffraction
    Guilhem Pages
    School of Molecular and Microbial Biosciences, University of Sydney, Sydney 2006 NSW, Australia
    J Magn Reson Imaging 28:1409-16. 2008
    ....
  56. doi request reprint "Setting paint" analogy for the hydrophobic self-association of tropoelastin into elastin-like hydrogel
    Christoph Naumann
    School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia
    Biopolymers 91:321-30. 2009
    ..In the present case, the sedimentation and entanglement of the tropoelastin porous sheets means that reverse dissolution is a kinetically slow process...
  57. pmc D-amino acid residue in a defensin-like peptide from platypus venom: effect on structure and chromatographic properties
    Allan M Torres
    School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia
    Biochem J 391:215-20. 2005
    ..Our results suggest that the incorporation of the D-amino acid at position 2 has minimal effect on the overall fold in solution...
  58. doi request reprint Mathematical models of naturally "morphed" human erythrocytes: stomatocytes and echinocytes
    Timothy J Larkin
    School of Molecular and Microbial Biosciences, Building G08, University of Sydney, Sydney, NSW, 2006, Australia
    Bull Math Biol 72:1323-33. 2010
    ....
  59. pmc Glutathione synthesis and turnover in the human erythrocyte: alignment of a model based on detailed enzyme kinetics with experimental data
    Julia E Raftos
    Department of Biological Sciences, Macquarie University, Sydney, New South Wales 2109, Australia
    J Biol Chem 285:23557-67. 2010
    ....
  60. pmc Defensins and the convergent evolution of platypus and reptile venom genes
    Camilla M Whittington
    Faculty of Veterinary Science, University of Sydney, Sydney, New South Wales 2006, Australia
    Genome Res 18:986-94. 2008
    ..Convergent evolution has repeatedly selected genes coding for proteins containing specific structural motifs as templates for venom molecules...
  61. ncbi request reprint Tunable-alignment chiral system based on gelatin for NMR spectroscopy
    Christoph Naumann
    School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia
    J Am Chem Soc 129:5340-1. 2007
  62. ncbi request reprint Scalar couplings as pH probes in compartmentalized biological systems: 31P NMR of phosphite
    Thomas R Eykyn
    School of Molecular and Microbial Biosciences, University of Sydney, Sydney, Australia
    Magn Reson Med 50:693-6. 2003
    ..01 pH units in the range of pH 5-7. Variations in erythrocyte pH due to metabolic changes are clearly observed...
  63. ncbi request reprint Rapid exchange of fluoroethylamine via the Rhesus complex in human erythrocytes: 19F NMR magnetization transfer analysis showing competition by ammonia and ammonia analogues
    David Szekely
    School of Molecular and Microbial Biosciences, University of Sydney, NSW 2006, Australia
    Biochemistry 45:9354-61. 2006
    ..Inhibition of the exchange by incubating the suspension with anti-RhAG antibody, and no inhibition by anti-RhD antibody, suggested specificity of exchange via the RhAG protein of the Rh complex...
  64. doi request reprint Mammalian peptide isomerase: platypus-type activity is present in mouse heart
    Jennifer M S Koh
    School of Molecular Biosciences, Building G08, University of Sydney, NSW 2006, Australia
    Chem Biodivers 7:1603-11. 2010
    ..Accordingly, eight mouse tissues were tested and heart was shown to have the activity. This is notable for being the first evidence of a peptide isomerase being present in a higher mammal and heralds finding the activity in man...
  65. ncbi request reprint Mammalian l-to-d-amino-acid-residue isomerase from platypus venom
    Allan M Torres
    School of Molecular and Microbial Biosciences, University of Sydney, Building G08, Sydney, NSW 2006, Australia
    FEBS Lett 580:1587-91. 2006
    ..The isomerase is approximately 50-60 kDa and is inhibited by methanol and the peptidase inhibitor amastatin. This is the first known l-to-d-amino-acid-residue isomerase in a mammal...
  66. doi request reprint Magnetic-resonance evaluation of the suitability of microstructured polymer optical fibers as sensors for ionic aqueous solutions
    Felicity M Cox
    Optical Fibre Technology Centre and School of Molecular and Microbial Biosciences, University of Sydney, New South Wales 2006, Australia, and School of Physical and Chemical Sciences, Queensland University of Technology, GPO Box 2434, Brisbane, Queensland 4001, Australia
    ACS Appl Mater Interfaces 1:197-203. 2009
    ..The findings suggest that microstructured PMMA fibers are compatible with ionic aqueous solutions and could provide a robust and durable platform for chemical-sensing applications...
  67. doi request reprint Effectors of the frequency of calcium oscillations in HEK-293 cells: wavelet analysis and a computer model
    David Szekely
    School of Molecular and Microbial Biosciences, University of Sydney, Building G08, Sydney, NSW, 2006, Australia
    Eur Biophys J 39:149-65. 2009
    ....
  68. doi request reprint Role of N-acetylcysteine and cystine in glutathione synthesis in human erythrocytes
    Stephney Whillier
    Department of Biological Sciences, Macquarie University, Sydney, New South Wales, Australia
    Redox Rep 14:115-24. 2009
    ..We conclude that the mechanism of action of therapeutically administered N-acetylcysteine is to reduce plasma cystine to cysteine that then enters the RBC and sustains glutathione synthesis...
  69. ncbi request reprint The Gárdos channel: a review of the Ca2+-activated K+ channel in human erythrocytes
    Anthony D Maher
    School of Molecular and Microbial Biosciences G08, University of Sydney, NSW 2006, Australia
    Int J Biochem Cell Biol 35:1182-97. 2003
    ....
  70. doi request reprint Structure of the pore-helix of the hERG K(+) channel
    Guilhem Pages
    School of Molecular and Microbial Biosciences, University of Sydney, Sydney, NSW, 2006, Australia
    Eur Biophys J 39:111-20. 2009
    ....
  71. doi request reprint Kinetics of in vitro digestion of starches monitored by time-resolved (1)H Nuclear Magnetic Resonance
    Anthony C Dona
    School of Molecular and Microbial Biosciences, University of Sydney, Sydney, NSW, Australia
    Biomacromolecules 10:638-44. 2009
    ..6 +/- 0.2) x 10(-2) mmol L(-1) s(-1) for the first stage of digestion, and (9.0 +/- 1.0) x 10(-3) and (4.7 +/- 1.4) x 10(-3) mmol L(-1) s(-1) for the second stage, giving a ratio of the two V(max) values of 2.1 and 3.4, respectively...
  72. ncbi request reprint Substrate specificity of platypus venom L-to-D-peptide isomerase
    Paramjit S Bansal
    School of Molecular and Microbial Biosciences, University of Sydney, New South Wales 2006, Australia
    J Biol Chem 283:8969-75. 2008
    ..A consistent 2-base mechanism is proposed for the isomerization; abstraction of a proton by 1 base is concomitant with delivery of a proton by the conjugate acid of a second base...
  73. ncbi request reprint Identification of a novel family of proteins in snake venoms. Purification and structural characterization of nawaprin from Naja nigricollis snake venom
    Allan M Torres
    School of Molecular and Microbial Biosciences, University of Sydney, Sydney, New South Wales 2006, Australia
    J Biol Chem 278:40097-104. 2003
    ..The amino acid residues in these important regions in nawaprin are different from those in elafin, suggesting that nawaprin is not an elastase-specific inhibitor and therefore has a different function in the snake venom...
  74. doi request reprint Expression patterns of platypus defensin and related venom genes across a range of tissue types reveal the possibility of broader functions for OvDLPs than previously suspected
    Camilla M Whittington
    Faculty of Veterinary Science, RMC Gunn B19, University of Sydney, NSW 2006, Australia
    Toxicon 52:559-65. 2008
    ....
  75. ncbi request reprint Rapid simulation and analysis of isotopomer distributions using constraints based on enzyme mechanisms: an example from HT29 cancer cells
    Vitaly A Selivanov
    Departamento de Bioquimica i Biologia Molecular, Facultat de Quimica and CERQT at Parc Cientic de Barcelona, Barcelona, Catalunya, Spain
    Bioinformatics 21:3558-64. 2005
    ..The mathematical relationships between kinetic and equilibrium constants restrict the domain of estimated fluxes to the ones compatible not only with a given set of experimental data, but also with other biochemical information...
  76. ncbi request reprint Simulations of NMR-detected diffusion in suspensions of red cells: the effects of variation in membrane permeability and observation time
    David G Regan
    Graduate School of Biomedical Engineering, University of New South Wales, NSW 2052, Australia
    Eur Biophys J 32:671-5. 2003
    ....
  77. ncbi request reprint Kinetics of uptake and deacetylation of N-acetylcysteine by human erythrocytes
    Julia E Raftos
    Department of Biological Sciences, Macquarie University, NSW 2109, Australia
    Int J Biochem Cell Biol 39:1698-706. 2007
    ..To be effective, intracellular cysteine precursors must be designed to enter erythrocytes rapidly and employ enzymes with high activity within erythrocytes to liberate the cysteine...
  78. ncbi request reprint Nobel Prizes for magnetic resonance imaging and channel proteins
    Jamie I Vandenberg
    Electrophysiology and Biophysics Unit, Victor Chang Cardiac Research Institute, Darlinghurst, NSW, Australia
    Med J Aust 179:611-3. 2003
  79. doi request reprint Inhomogeneous NMR line shape as a probe of microscopic organization of bicontinuous cubic phases
    Konstantin I Momot
    School of Physical and Chemical Sciences, Queensland University of Technology, GPO Box 2434, Brisbane, Qld 4001, Australia
    J Phys Chem B 112:6636-45. 2008
    ..We discuss how this inhomogeneous broadening can be employed as a probe of compositional uniformity and microscopic organization of BCP samples...