Hossein Baharvand

Summary

Publications

  1. ncbi The ultrastructure of mouse embryonic stem cells
    Hossein Baharvand
    Department of Embryology, Royan Institute, PO Box 19395 4644, Tehran, Iran
    Reprod Biomed Online 7:330-5. 2003
  2. ncbi Neural differentiation from human embryonic stem cells in a defined adherent culture condition
    Hossein Baharvand
    Department of Stem Cells, Royan Institute, Tehran, Iran
    Int J Dev Biol 51:371-8. 2007
  3. ncbi Human amniotic membrane as a suitable matrix for growth of mouse urothelial cells in comparison with human peritoneal and omentum membranes
    Farzaneh Sharifiaghdas
    Urology and Nephrology Research Center and Department of Urology, Shaheed Labbafinejad Medical Center, Shaheed Beheshti Medical University, Pasdaran, Tehran, Iran
    Urol J 4:71-8. 2007
  4. ncbi Identification of mouse embryonic stem cell-associated proteins
    Hossein Baharvand
    Department of Stem Cells, Royan Institute, Tehran, Iran
    J Proteome Res 7:412-23. 2008
  5. pmc Phase 1 human trial of autologous bone marrow-hematopoietic stem cell transplantation in patients with decompensated cirrhosis
    Mehdi Mohamadnejad
    Department of Stem Cells, Royan Institute, PO Box 19395 4644, Tehran, Iran
    World J Gastroenterol 13:3359-63. 2007
  6. ncbi Concise review: trends in stem cell proteomics
    Hossein Baharvand
    Department of Stem Cells, Royan Institute, Tehran, Iran
    Stem Cells 25:1888-903. 2007
  7. ncbi Comparison of characteristics of cultured limbal cells on denuded amniotic membrane and fresh conjunctival, limbal and corneal tissues
    Hossein Baharvand
    Department of Stem Cells, Royan Institute, PO Box 19395 4644, Tehran, Iran
    Dev Growth Differ 49:241-51. 2007
  8. doi Differentiation of human embryonic stem cells into functional hepatocyte-like cells in a serum-free adherent culture condition
    Hossein Baharvand
    Department of Stem Cells Royan Institute, P O Box 19395 4644 Tehran, Iran
    Differentiation 76:465-77. 2008
  9. ncbi Comparative proteomic analysis of mouse embryonic stem cells and neonatal-derived cardiomyocytes
    Hossein Baharvand
    Department of Stem Cells, Royan Institute, Tehran, Iran
    Biochem Biophys Res Commun 349:1041-9. 2006
  10. ncbi Differentiation of human embryonic stem cells into hepatocytes in 2D and 3D culture systems in vitro
    Hossein Baharvand
    Department of Stem Cells, Royan Institute, Tehran, Iran
    Int J Dev Biol 50:645-52. 2006

Detail Information

Publications93

  1. ncbi The ultrastructure of mouse embryonic stem cells
    Hossein Baharvand
    Department of Embryology, Royan Institute, PO Box 19395 4644, Tehran, Iran
    Reprod Biomed Online 7:330-5. 2003
    ..These results are consistent with the ultrastructural features of undifferentiated cells...
  2. ncbi Neural differentiation from human embryonic stem cells in a defined adherent culture condition
    Hossein Baharvand
    Department of Stem Cells, Royan Institute, Tehran, Iran
    Int J Dev Biol 51:371-8. 2007
    ..The culture of neural precursor cells in a collagen scaffold may provide a new approach for the repair of spinal cord injury...
  3. ncbi Human amniotic membrane as a suitable matrix for growth of mouse urothelial cells in comparison with human peritoneal and omentum membranes
    Farzaneh Sharifiaghdas
    Urology and Nephrology Research Center and Department of Urology, Shaheed Labbafinejad Medical Center, Shaheed Beheshti Medical University, Pasdaran, Tehran, Iran
    Urol J 4:71-8. 2007
    ..The aim of the present study was to assess the proliferation quality of mouse urothelial cells on 3 natural matrixes of human amniotic membrane (AM), peritoneum, and omentum, and to compare them with collagen matrix...
  4. ncbi Identification of mouse embryonic stem cell-associated proteins
    Hossein Baharvand
    Department of Stem Cells, Royan Institute, Tehran, Iran
    J Proteome Res 7:412-23. 2008
    ..Several novel ESC-associated proteins have been presented in this study which warrants further investigation with respect to the etiology of stemness...
  5. pmc Phase 1 human trial of autologous bone marrow-hematopoietic stem cell transplantation in patients with decompensated cirrhosis
    Mehdi Mohamadnejad
    Department of Stem Cells, Royan Institute, PO Box 19395 4644, Tehran, Iran
    World J Gastroenterol 13:3359-63. 2007
    ..To evaluate safety and feasibility of autologous bone marrow-enriched CD34+ hematopoietic stem cell Tx through the hepatic artery in patients with decompensated cirrhosis...
  6. ncbi Concise review: trends in stem cell proteomics
    Hossein Baharvand
    Department of Stem Cells, Royan Institute, Tehran, Iran
    Stem Cells 25:1888-903. 2007
    ..Disclosure of potential conflicts of interest is found at the end of this article...
  7. ncbi Comparison of characteristics of cultured limbal cells on denuded amniotic membrane and fresh conjunctival, limbal and corneal tissues
    Hossein Baharvand
    Department of Stem Cells, Royan Institute, PO Box 19395 4644, Tehran, Iran
    Dev Growth Differ 49:241-51. 2007
    ..Also, the expression of traditional SC markers may not be a reliable indicator of limbal SC and there is an increasing need to determine factor(s) involved in their stemness...
  8. doi Differentiation of human embryonic stem cells into functional hepatocyte-like cells in a serum-free adherent culture condition
    Hossein Baharvand
    Department of Stem Cells Royan Institute, P O Box 19395 4644 Tehran, Iran
    Differentiation 76:465-77. 2008
    ....
  9. ncbi Comparative proteomic analysis of mouse embryonic stem cells and neonatal-derived cardiomyocytes
    Hossein Baharvand
    Department of Stem Cells, Royan Institute, Tehran, Iran
    Biochem Biophys Res Commun 349:1041-9. 2006
    ..This study provides a new insight into the gene expression pattern of differentiated cardiomyocytes and is further evidence for a close relation between ESC-DCs and N-DCSs...
  10. ncbi Differentiation of human embryonic stem cells into hepatocytes in 2D and 3D culture systems in vitro
    Hossein Baharvand
    Department of Stem Cells, Royan Institute, Tehran, Iran
    Int J Dev Biol 50:645-52. 2006
    ....
  11. ncbi Ultrastructural comparison of developing mouse embryonic stem cell- and in vivo-derived cardiomyocytes
    Hossein Baharvand
    Department of Stem Cells, Royan Institute, Tehran, Islamic Republic of Iran
    Cell Biol Int 30:800-7. 2006
    ..Our data suggest that ESCs can differentiate into functional mature CMs in vitro. Furthermore, ESC-CMs may provide an ideal model for the study of cardiomyocytic development and may be useful for cell therapy of various cardiac diseases...
  12. ncbi Generation of insulin-secreting cells from human embryonic stem cells
    Hossein Baharvand
    Department of Stem Cells, Royan Institute, PO Box 19395 4644, Tehran, Iran
    Dev Growth Differ 48:323-32. 2006
    ..The results showed that hESC have the ability to transcribe and process insulin, but further improvements of the current method are required to generate a sufficient source of true beta cells for the treatment of diabetes mellitus...
  13. ncbi Proteomic signature of human embryonic stem cells
    Hossein Baharvand
    Department of Stem Cells, Royan Institute, Tehran, Iran
    Proteomics 6:3544-9. 2006
    ..To our knowledge, this study represents the first proteomic dataset for hESC and provides a better insight into the biology of hESC. Proteome maps of hESC are accessible at http://www.RoyanProteomics.ir...
  14. ncbi Generation of new human embryonic stem cell lines with diploid and triploid karyotypes
    Hossein Baharvand
    Department of Stem Cells, Royan Institute, Tehran, Iran
    Dev Growth Differ 48:117-28. 2006
    ..Whereas all of the cell lines expressed all the characteristics of undifferentiated pluripotent hESC, two of the cell lines carried a triploid karyotype...
  15. ncbi The effect of extracellular matrix on embryonic stem cell-derived cardiomyocytes
    Hossein Baharvand
    Department of Biology, University for Teacher Training, Tehran, Iran
    J Mol Cell Cardiol 38:495-503. 2005
    ....
  16. ncbi Culture condition difference for establishment of new embryonic stem cell lines from the C57BL/6 and BALB/c mouse strains
    Hossein Baharvand
    Department for Biology of Stem Cells and Department of Embryology, Royan Institute, P O Box 19395 4644, Tehran, Iran
    In Vitro Cell Dev Biol Anim 40:76-81. 2004
    ..These results indicate that ES cells from inbred strains can be isolated using commercially available reagents and that the establishment of BALB/c ES cell lines may require different culture conditions to the 129 or C57BL/6 strains...
  17. ncbi Establishment and in vitro differentiation of a new embryonic stem cell line from human blastocyst
    Hossein Baharvand
    Department for Biology of Stem Cells, Royan Institute, P O Box 19395 4644, Tehran, Iran
    Differentiation 72:224-9. 2004
    ..These results define Royan H1 cells as a new human embryonic stem cell line...
  18. ncbi Effect of poly-L-lysine coating on retinoic acid-loaded PLGA microspheres in the differentiation of carcinoma stem cells into neural cells
    Hanieh Nojehdehian
    Stem Cell Department, Cell Science Research Center, Royan Institute, Tehran, Iran
    Int J Artif Organs 33:721-30. 2010
    ..Finally, these results highlight the versatility of these sample scaffolds as a model system for nerve tissue engineering...
  19. doi A simple and efficient cryopreservation method for feeder-free dissociated human induced pluripotent stem cells and human embryonic stem cells
    Sepideh Mollamohammadi
    Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, PO Box 19395 4644, Tehran, Iran
    Hum Reprod 24:2468-76. 2009
    ..An essential prerequisite for the future widespread application of human induced pluripotent (hiPSCs) and embryonic stem cells (hESCs) is the development of efficient cryopreservation methods to facilitate their storage and transportation...
  20. doi Presence of a ROCK inhibitor in extracellular matrix supports more undifferentiated growth of feeder-free human embryonic and induced pluripotent stem cells upon passaging
    Mohammad Pakzad
    Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, P O Box 19395 4644, Tehran, Iran
    Stem Cell Rev 6:96-107. 2010
    ..This is due not only to an anti-apoptotic effect, but also to an increase in the ECM-cells interaction. Therefore, we believe this method will be useful for both current and future applications of these pluripotent stem cells...
  21. doi Generation of functional hepatocyte-like cells from human pluripotent stem cells in a scalable suspension culture
    Massoud Vosough
    1 Department of Biochemistry, Pasteur Institute of Iran, Tehran, Iran
    Stem Cells Dev 22:2693-705. 2013
    ..This amenable scaling up and outlined enrichment strategy provides a new platform for generating functional HLCs. This integrated approach may facilitate biomedical applications of the hPSC-derived hepatocytes. ..
  22. doi Glycogen synthase kinase-3 inhibition promotes proliferation and neuronal differentiation of human-induced pluripotent stem cell-derived neural progenitors
    Fereshteh Esfandiari
    Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
    Stem Cells Dev 21:3233-43. 2012
    ..This may provide desired cells that can be used in therapeutic applications and offer insights into the etiology of some neurological disorders...
  23. doi Enhanced generation of human embryonic stem cells from single blastomeres of fair and poor-quality cleavage embryos via inhibition of glycogen synthase kinase β and Rho-associated kinase signaling
    Adeleh Taei
    Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, P O Box 19395 4644, Tehran, Iran
    Hum Reprod 28:2661-71. 2013
    ..Could selected pluripotency-enhancing small molecules (SMs) lead to efficient derivation of human embryonic stem cells (hESCs) from cleavage embryos-derived single blastomeres (SBs)?..
  24. doi Interactions of human embryonic stem cell-derived neural progenitors with an electrospun nanofibrillar surface in vitro
    Ali Rahjouei
    Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
    Int J Artif Organs 34:559-70. 2011
    ..The results suggest that topographical features of the extracellular matrix of the cell environment have paved the way for a better understanding of human neuronal development, thus allowing for future clinical applications...
  25. doi Long-term self-renewable feeder-free human induced pluripotent stem cell-derived neural progenitors
    Shiva Nemati
    Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
    Stem Cells Dev 20:503-14. 2011
    ..The characteristics of our generated hiPSC-NPs provide the opportunity to use patient-specific or ready-to-use hiPSC-NPs in future biomedical applications...
  26. doi Feeder- and serum-free establishment and expansion of human induced pluripotent stem cells
    Mehdi Totonchi
    Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
    Int J Dev Biol 54:877-86. 2010
    ..Such hiPSCs could be useful particularly in the context of in vitro disease modeling, pharmaceutical screening and in cellular replacement therapies once the safety issues have been overcome...
  27. doi Generation of liver disease-specific induced pluripotent stem cells along with efficient differentiation to functional hepatocyte-like cells
    Arefeh Ghodsizadeh
    Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, P O Box 19395 4644, Tehran, Iran
    Stem Cell Rev 6:622-32. 2010
    ..Our results provide proof of principal that human liver-disease specific iPSCs present an exciting potential venue toward cell-based therapeutics, drug metabolism, human liver development and disease models for liver failure disorders...
  28. doi Disease-corrected hepatocyte-like cells from familial hypercholesterolemia-induced pluripotent stem cells
    Faranak Fattahi
    Department of Molecular Systems Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
    Mol Biotechnol 54:863-73. 2013
    ..These data suggest that hiPSC technology can be used for generation of disease-corrected, patient-specific HLCs with potential value for disease modeling and drug discovery as well as cell therapy applications in future...
  29. doi A new efficient protocol for directed differentiation of retinal pigmented epithelial cells from normal and retinal disease induced pluripotent stem cells
    Azadeh Zahabi
    Department of Stem Cells and Developmental Biology, The Academic Center for Education, Culture and Research ACECR, Tehran, Iran
    Stem Cells Dev 21:2262-72. 2012
    ....
  30. doi Simultaneous suppression of TGF-β and ERK signaling contributes to the highly efficient and reproducible generation of mouse embryonic stem cells from previously considered refractory and non-permissive strains
    Seyedeh Nafiseh Hassani
    Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, PO Box 19395 4644, Tehran, Iran
    Stem Cell Rev 8:472-81. 2012
    ..The application of these two inhibitors will not only solve the problems of mESC derivation but also clarifies new signaling pathways in pluripotent mESCs...
  31. doi Differentiation of bone marrow-derived mesenchymal stem cells into hepatocyte-like cells on nanofibers and their transplantation into a carbon tetrachloride-induced liver fibrosis model
    Abbas Piryaei
    Department of Anatomy, Faculty of Medical Sciences, Tarbiat Modares University, PO Box 14115 111, Tehran, Iran
    Stem Cell Rev 7:103-18. 2011
    ..These results showed that topographic properties of nanofibers enhance differentiation of HLCs from MSCs and maintain their function in long-term culture, which has implications for cell therapies...
  32. doi Human-induced pluripotent stem cells: derivation, propagation, and freezing in serum- and feeder layer-free culture conditions
    Hossein Baharvand
    Department of Stem Cells, Cell Science Research Center, Royan Institute, ACECR, Tehran, Iran
    Methods Mol Biol 584:425-43. 2010
    ..Our findings greatly simplify the method for induction of pluripotency and bring it one step closer to clinical applications. Moreover, the established hiPS cells showed chromosomal stability during long-term culture...
  33. doi Long-term maintenance of undifferentiated human embryonic and induced pluripotent stem cells in suspension
    Mehran Rezaei Larijani
    Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, The Academic Center for Education, Culture and Research, Tehran, Iran
    Stem Cells Dev 20:1911-23. 2011
    ..Our results will pave the way for scaled up expansion and controlled differentiation of hESCs and hiPSCs needed for cell therapy, research, and industrial applications in a bioreactor culture system...
  34. doi Neural induction in mouse embryonic stem cells by co-culturing with chicken somites
    Mohsen Sagha
    Department of Stem Cells, Cell Science Research Center, Royan Institute, Esfahan Campus, Iranian Academic Center for Education, Culture, and Research ACECR, Esfahan, Iran
    Stem Cells Dev 18:1351-60. 2009
    ..However, no effective influence of somitic co-culture on rostrocaudal or dorsoventral patterning is seen. Therefore, like amphibians, somites have a modest neural-inducing activity in amniotes...
  35. doi Effects of different extracellular matrices and co-cultures on human limbal stem cell expansion in vitro
    Naghmeh Ahmadiankia
    Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
    Cell Biol Int 33:978-87. 2009
    ..In conclusion, the cultivation of human limbal explants on AM co-culturing with human LFs promises to be a good model for preparing undifferentiated epithelial sheets suitable for transplantation...
  36. doi Repeated Intraportal Injection of Mesenchymal Stem Cells in Combination with Pioglitazone in Patients with Compensated Cirrhosis: A Clinical Report of Two Cases
    Massoud Vosough
    Department of Regenerative Medicine, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
    Arch Iran Med 19:131-6. 2016
    ..In this study, we conducted a pilot study of intraportal infusion of autologous MSCs in combination with pioglitazone to assess safety, feasibility, and effectiveness in patients with compensated cirrhosis...
  37. doi An efficient and easy-to-use cryopreservation protocol for human ES and iPS cells
    Hossein Baharvand
    Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
    Nat Protoc 5:588-94. 2010
    ..The treatment with ROCK inhibitor, cell harvesting and the freezing-thawing process usually takes about 2 h excluding overnight incubation at -80 degrees C...
  38. doi Nuclear proteome analysis of monkey embryonic stem cells during differentiation
    Davood Nasrabadi
    Department of Molecular Systems Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, P O Box 19395 4644, Karaj, Iran
    Stem Cell Rev 6:50-61. 2010
    ..Functional interaction analysis of differentially expressed proteins revealed that most of nuclear proteins had a direct interaction with c-Myc and p53...
  39. doi Proteome analysis of post-transplantation recovery mechanisms of an EAE model of multiple sclerosis treated with embryonic stem cell-derived neural precursors
    Abolhassan Shahzadeh Fazeli
    Department of Molecular Systems Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran Department of Genetics at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
    J Proteomics 94:437-50. 2013
    ..Our results provided a proteomic view of the molecular mechanisms of EAE recovery after stem cell transplantation...
  40. doi Dehydroepiandrosterone stimulates neurogenesis in mouse embryonal carcinoma cell- and human embryonic stem cell-derived neural progenitors and induces dopaminergic neurons
    Hossein Azizi
    Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, University of Science and Culture, ACECR, Tehran, Islamic Republic of Iran
    Stem Cells Dev 19:809-18. 2010
    ..This provides a better insight into the differentiation and maintenance of neural cells and treatment of a wide variety of neurological diseases such as Alzheimer's and Parkinson's by stem cells...
  41. ncbi Differential effect of activin on mouse embryonic stem cell differentiation in insulin-secreting cells under nestin-positive selection and spontaneous differentiation protocols
    Hanieh Jafary
    Department of Stem Cells, Royan Institute, PO Box 19395 4644, Tehran, Iran
    Cell Biol Int 32:278-86. 2008
    ..However, improvements of the current method are required to generate a sufficient source of true beta-cells for the treatment of diabetes mellitus...
  42. doi Midterm outcomes of autologous cultivated limbal stem cell transplantation with or without penetrating keratoplasty
    Alireza Baradaran-Rafii
    Ophthalmic Research Center, Labbafinejad Hospital, Shahid Beheshti University MC, Tehran, Iran
    Cornea 29:502-9. 2010
    ....
  43. pmc In vitro maturation, apoptotic gene expression and incidence of numerical chromosomal abnormalities following cryotop vitrification of sheep cumulus-oocyte complexes
    Bita Ebrahimi
    Department of Anatomy, Faculty of Medical Science, Tarbiat Modares University, Tehran, Iran
    J Assist Reprod Genet 27:239-46. 2010
    ..The purpose of this study was to evaluate the effects of cryotop vitrification of sheep cumulus-oocyte complexes (COCs) on oocyte maturation, apoptotic gene expression and incidence of chromosomal abnormalities...
  44. doi Potential role of heat shock proteins in neural differentiation of murine embryonal carcinoma stem cells (P19)
    Elahe Afzal
    Department of Regenerative Medicine, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
    Cell Biol Int 35:713-20. 2011
    ..In conclusion, our results raise the possibility that HSPs could regulate cell differentiation and proliferation under both physiological and pathological conditions...
  45. doi Improvement of islet engrafts by enhanced angiogenesis and microparticle-mediated oxygenation
    Leila Montazeri
    Department of Biomedical Engineering, Amirkabir University of Technology, Tehran, Iran
    Biomaterials 89:157-65. 2016
    ..These results suggest that the developed platform has great potential to enhance the efficacy for implants in cases where the cell dosage is critical for efficacy, such as islet transplantation and ischemic tissues...
  46. doi Differentiation and transplantation of human induced pluripotent stem cell-derived hepatocyte-like cells
    Samira Asgari
    Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, PO Box 19395 4644, Tehran, Iran
    Stem Cell Rev 9:493-504. 2013
    ..These results may bring new insights in the clinical applications of hiPSCs once safety issues are overcome...
  47. pmc Engrafted human induced pluripotent stem cell-derived anterior specified neural progenitors protect the rat crushed optic nerve
    Leila Satarian
    Department of Physiology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
    PLoS ONE 8:e71855. 2013
    ....
  48. doi A new chemical approach to the efficient generation of mouse embryonic stem cells
    Hossein Baharvand
    Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
    Methods Mol Biol 997:13-22. 2013
    ..The efficiency of mESC lines generation is 100%, regardless of genetic background...
  49. doi Retinoic acid dependent histone 3 demethylation of the clustered HOX genes during neural differentiation of human embryonic stem cells
    Maryam Shahhoseini
    Department of Genetics at Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, ACECR, P O Box 19395 4644, Tehran, Iran
    Biochem Cell Biol 91:116-22. 2013
    ..This finding clearly showed the functional role of UTX in epigenetic alteration of HOX clusters during RA-induced neural differentiation; the activity could not be detectable for the demethylase JMJD3 during this developmental process...
  50. pmc ISL1 protein transduction promotes cardiomyocyte differentiation from human embryonic stem cells
    Hananeh Fonoudi
    Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, Tehran, Iran
    PLoS ONE 8:e55577. 2013
    ..Protein transduction has been demonstrated as an alternative approach for increasing the efficiency of hESCs differentiation toward cardiomyocytes...
  51. pmc Human induced pluripotent stem cells differentiation into oligodendrocyte progenitors and transplantation in a rat model of optic chiasm demyelination
    Alireza Pouya
    Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
    PLoS ONE 6:e27925. 2011
    ..This study aims to differentiate human induced pluripotent stem cells (hiPSCs) into oligodendrocyte precursors and assess their recovery potential in a demyelinated optic chiasm model in rats...
  52. doi Differentiation of embryonic stem cells into neural cells on 3D poly (D, L-lactic acid) scaffolds versus 2D cultures
    Narges Zare-Mehrjardi
    Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
    Int J Artif Organs 34:1012-23. 2011
    ..The results of this study demonstrated a promising effect of 3D scaffold culture for neural cell differentiation from ESCs in prospective tissue engineering applications...
  53. doi Electrospun nanofibrillar surfaces promote neuronal differentiation and function from human embryonic stem cells
    Ebrahim Shahbazi
    Department of Stem Cells and Development al Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
    Tissue Eng Part A 17:3021-31. 2011
    ....
  54. doi In vitro differentiation of human bone marrow mesenchymal stem cells into hepatocyte-like cells
    Behshad Pournasr
    Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, Tehran, Iran
    Arch Iran Med 14:244-9. 2011
    ..We aimed to investigate the differentiation potential of BM-MSCs into hepatocyte-like cells...
  55. ncbi Phase 1 trial of autologous bone marrow mesenchymal stem cell transplantation in patients with decompensated liver cirrhosis
    Mehdi Mohamadnejad
    Digestive Disease Research Center, Medical Sciences Tehran University, Tehran, Iran
    Arch Iran Med 10:459-66. 2007
    ..The objective of this study was to determine the safety and feasibility of autologous bone marrow-mesenchymal stem cell transplantation in patients with decompensated liver cirrhosis...
  56. doi Epigenetic analysis of human embryonic carcinoma cells during retinoic acid-induced neural differentiation
    Maryam Shahhoseini
    Department of Genetics, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
    Biochem Cell Biol 88:527-38. 2010
    ..This report demonstrates the dynamic interplay of histone modification and variation in regulating the gene expression profile, during stem cell differentiation and under different culture conditions...
  57. doi Mesenchymal stem cell infusion therapy in a carbon tetrachloride-induced liver fibrosis model affects matrix metalloproteinase expression
    Vahideh Rabani
    Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
    Cell Biol Int 34:601-5. 2010
    ..In conclusion, MSCs can enhance recovery of a CCl(4)-injured mouse liver through their influence in reducing collagen deposition by possibly affecting expression of MMPs...
  58. doi Derivation of new human embryonic stem cell lines from preimplantation genetic screening and diagnosis-analyzed embryos
    Adeleh Taei
    Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, P O Box 19395 4644, Tehran, Iran
    In Vitro Cell Dev Biol Anim 46:395-402. 2010
    ..These results further indicate that hESC lines can be derived from PGD/PGS-analyzed embryos that are destined to be discarded and can serve as an alternative source for normal euploid lines...
  59. doi Preparation and surface characterization of poly-L-lysine-coated PLGA microsphere scaffolds containing retinoic acid for nerve tissue engineering: in vitro study
    Hanieh Nojehdehian
    Stem Cells Department, Cell Science Research Center, Royan Institute, P O Box 19395 4644, Tehran, Iran
    Colloids Surf B Biointerfaces 73:23-9. 2009
    ..8% and 72+/-6.9% of the cells expressed beta-tubulin III on the surface of coated and uncoated RA-loaded PLGA microspheres, respectively, while the 64+/-1.1% (P < 0.05) cells expressed tubulin III in group with soluble...
  60. ncbi The thickness of electrospun poly (epsilon-caprolactone) nanofibrous scaffolds influences cell proliferation
    Laleh Ghasemi-Mobarakeh
    Department of Stem Cells, Cell Science Research Center, Royan Institute, Esfahan Campus, ACECR, Esfahan Iran
    Int J Artif Organs 32:150-8. 2009
    ..Therefore, regardless of cell origin, thicker scaffolds provide a better substrate for cell proliferation, possibly due to the higher dimensional stability and tightness of thicker scaffolds...
  61. doi Substrate-mediated commitment of human embryonic stem cells for hepatic differentiation
    Mohammad Kazemi Ashtiani
    Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
    J Biomed Mater Res A 104:2861-72. 2016
    ..We also demonstrated that iAct in the presence of soluble Act (sAct) could improve the conventional protocol to generate HLCs from hESCs. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 2861-2872, 2016. ..
  62. doi The behavior of cardiac progenitor cells on macroporous pericardium-derived scaffolds
    Sareh Rajabi-Zeleti
    Department of Biology, Kharazmi University, Tehran, Iran Department of Stem Cells and Developmental Biology at Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
    Biomaterials 35:970-82. 2014
    ..This research demonstrates the feasibility of fabricating 3D porous scaffolds from native ECMs and suggests the therapeutic potential of CPC-seeded PSs in the treatment of ischemic heart diseases. ..
  63. doi Mesenchymal stem cell-conditioned medium accelerates regeneration of human renal proximal tubule epithelial cells after gentamicin toxicity
    Reza Moghadasali
    Department of Biology, Tarbiat Moallem University, Tehran, Iran
    Exp Toxicol Pathol 65:595-600. 2013
    ..Our data suggest that cytokines secreted by MSCs stimulate renal tubule cell regeneration after nephrotoxicity...
  64. doi Treatment of human embryonic stem cells with different combinations of priming and inducing factors toward definitive endoderm
    Yasser Tahamtani
    Department of Biology, Kharazmi University, Tehran, Iran
    Stem Cells Dev 22:1419-32. 2013
    ..Thus, current protocols for the differentiation of DE into PE still need additional study...
  65. doi Bioprocess development for mass production of size-controlled human pluripotent stem cell aggregates in stirred suspension bioreactor
    Saeed Abbasalizadeh
    Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
    Tissue Eng Part C Methods 18:831-51. 2012
    ....
  66. pmc Quantitative proteomic analysis of human embryonic stem cell differentiation by 8-plex iTRAQ labelling
    Mahdieh Jadaliha
    Department of Molecular Systems Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
    PLoS ONE 7:e38532. 2012
    ..The present findings added insight to our understanding of the mechanisms involved in hESC proliferation and differentiation...
  67. doi Inhibition of glycogen synthase kinase-3 promotes efficient derivation of pluripotent stem cells from neonatal mouse testis
    Seyedeh Faezeh Moraveji
    Department of Stem Cells and Developmental Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, P O Box 19395 4644, Tehran, Iran
    Hum Reprod 27:2312-24. 2012
    ..To obtain a better and robust derivation, we have attempted to identify small molecules (SMs) that induce reprogramming of testicular cells in culture into germline-derived pluripotent stem cells (gPSCs)...
  68. doi An orthogonal comparison of the proteome of human embryonic stem cells with that of human induced pluripotent stem cells of different genetic background
    Mojtaba Zamani Faradonbeh
    Department of Molecular Systems Biology, Cell Science Research Center, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
    Mol Biosyst 8:1833-40. 2012
    ..Our results showed that hESCs and hiPSCs had subtle differences at the proteome level thus warranting more detailed and systematic examinations of these cells...
  69. doi Concise review: alchemy of biology: generating desired cell types from abundant and accessible cells
    Behshad Pournasr
    Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
    Stem Cells 29:1933-41. 2011
    ....
  70. doi Comparative proteome and transcriptome analyses of embryonic stem cells during embryoid body-based differentiation
    Ali Fathi
    Department of Molecular Systems Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
    Proteomics 9:4859-70. 2009
    ..Although regulation of many genes during differentiation were observed only at transcript level, modulation of several proteins was revealed only by proteome analysis...
  71. ncbi Kinetic properties of extracted lactate dehydrogenase and creatine kinase from mouse embryonic stem cell- and neonatal-derived cardiomyocytes
    Roseata Zonouzi
    Department of Stem Cells, Royan Institute, Tehran, Iran
    J Biochem Mol Biol 39:426-31. 2006
    ..In defining some kinetic properties of cardiac metabolic enzymes of ESC-derived cardiomyocytes, our results are expected to further facilitate the use of ESCs as an experimental model...
  72. doi Rat marrow-derived mesenchymal stem cells developed in a medium supplemented with the autologous versus bovine serum
    Mohamadreza Baghaban Eslaminejad
    Stem Cell Department, Cell Sciences Research Center, Royan Institute, ACECR, Tehran, Iran
    Cell Biol Int 33:607-16. 2009
    ..Our data indicated that AS enriched medium provided a microenvironment in which growth rate as well as bone differentiation of the isolated MSCs were significantly higher than in FBS enriched medium...
  73. doi Human embryonic stem cell-derived neural precursor transplants in collagen scaffolds promote recovery in injured rat spinal cord
    Maryam Hatami
    Department of Stem Cells, Cell Science Research Center, Royan Institute, Tehran, Iran
    Cytotherapy 11:618-30. 2009
    ..However, the potential of human embryonic stem cell-derived NPC (hESC-NPC) as a tool for cell replacement of spinal cord injury (SCI) should be considered...
  74. pmc Assessment of heat shock protein (HSP60, HSP72, HSP90, and HSC70) expression in cultured limbal stem cells following air lifting
    Marzeih Ebrahimi
    Department of Regenerative Medicine, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
    Mol Vis 16:1680-8. 2010
    ....
  75. doi Quantum dot labeling using positive charged peptides in human hematopoetic and mesenchymal stem cells
    Sarah Ranjbarvaziri
    Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
    Biomaterials 32:5195-205. 2011
    ..These data suggest that before any utilization of QDs in bio-imaging and related applications, an efficient intra-cellular delivery technique should be considered to preserve QDs for a prolonged time as well as eliminating their leakage...
  76. doi Induced pluripotent stem cells: a new era for hepatology
    Samira Asgari
    Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
    J Hepatol 53:738-51. 2010
    ..This focused review will discuss how human iPS cell advances are likely to have an impact on hepatology...
  77. doi Basic fibroblast growth factor potentiates myelin repair following induction of experimental demyelination in adult mouse optic chiasm and nerves
    Samaneh Dehghan
    Department of Physiology, Faculty of Medical Sciences, Tarbiat Modares University, P O Box 14115 331, Tehran, Iran
    J Mol Neurosci 48:77-85. 2012
    ..Its effect on demyelination and remyelination processes seems to be mediated by oligodendrocyte progenitor cells and their differentiation to myelinating cells...
  78. ncbi Proteomic analysis of epithelium-denuded human amniotic membrane as a limbal stem cell niche
    Hossein Baharvand
    Department of Stem Cells, Royan Institute, Tehran, Iran
    Mol Vis 13:1711-21. 2007
    ..We investigated the proteome of the epithelium-denuded AM to increase our understanding of the mechanisms by which AM may confer its beneficial effects...
  79. doi Effect of lead on proliferation and neural differentiation of mouse bone marrow-mesenchymal stem cells
    Shabnam Kermani
    Department of Stem Cells, Cell Science Research Center, Royan Institute, Esfahan Campus, PO Box 8158968433, Esfahan, Iran
    Toxicol In Vitro 22:995-1001. 2008
    ..Therefore, MSCs appear to be an alternative method for assessing the cytotoxic effects of such environmental hazards...
  80. doi Proteome analysis of brain in murine experimental autoimmune encephalomyelitis
    Abolhassan Shahzadeh Fazeli
    Department of Molecular Systems Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
    Proteomics 10:2822-32. 2010
    ..The possible role of these proteins in the responses of mice to animal models of multiple sclerosis is discussed...
  81. doi Neurogenic and mitotic effects of dehydroepiandrosterone on neuronal-competent marrow mesenchymal stem cells
    Esmaeil H Shiri
    Department of Stem Cells, Cell Science Research Center, ACECR, Royan Institute, Tehran, Iran
    Int J Dev Biol 53:579-84. 2009
    ....
  82. doi Enhanced functions of human embryonic stem cell-derived hepatocyte-like cells on three-dimensional nanofibrillar surfaces
    Zahra Farzaneh
    Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, P O Box 19395 4644, Tehran, Iran
    Stem Cell Rev 6:601-10. 2010
    ..These results show that topographically treated hESCs at the nano level have a distinct hepatic functionality profile which has implications for cell therapies...
  83. doi Generation of motor neurons by coculture of retinoic acid-pretreated embryonic stem cells with chicken notochords
    Maryam Anjomshoa
    Department of Stem Cells, Cell Science Research Center, Royan Institute, ACECR, Esfahan Campus, Esfahan, Iran
    Stem Cells Dev 18:259-67. 2009
    ....
  84. doi Generation of human induced pluripotent stem cells from a Bombay individual: moving towards "universal-donor" red blood cells
    Ali Seifinejad
    Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, PO Box 19395 4644, ACECR, Tehran, Iran
    Biochem Biophys Res Commun 391:329-34. 2010
    ..Such specific stem cells provide an unprecedented opportunity to produce a universal blood group donor, in-vitro, thus enabling cellular replacement therapies, once the safety issue is resolved...
  85. doi IVM and gene expression of sheep cumulus-oocyte complexes following different methods of vitrification
    Bita Ebrahimi
    Department of Anatomy, School of Medical Science, Tarbiat Modarres University, PO Box 14115 111, Tehran, Iran
    Reprod Biomed Online 20:26-34. 2010
    ..In conclusion, direct cryotop, when compared with other vitrification methods, seemed to be safe and could increase the viability, post-warming maturation and maturation-gene expression rates of sheep COC...
  86. doi Proteomic analysis of monkey embryonic stem cell during differentiation
    Davood Nasrabadi
    Department of Stem Cells, Cell Science Research Center, Royan Institute, ACECR, Tehran, Iran
    J Proteome Res 8:1527-39. 2009
    ..Taken together, the results presented here revealed novel key proteins and pathways that are active during ESC differentiation...
  87. doi Evidence for a dynamic role of the linker histone variant H1x during retinoic acid-induced differentiation of NT2 cells
    Maryam Shahhoseini
    Department of Genetics, Royan Institute for Reproductive Biomedicine, ACECR, Tehran, Iran
    FEBS Lett 584:4661-4. 2010
    ..This finding reveals a dynamic role of H1x in differentiation, and implies a repressive role for this histone variant...
  88. doi Safety and efficacy of granulocyte-colony-stimulating factor administration following autologous intramuscular implantation of bone marrow mononuclear cells: a randomized controlled trial in patients with advanced lower limb ischemia
    Mohammad Reza Zafarghandi
    Department of Vascular Surgery, Tehran University of Medical Sciences, Tehran, Iran
    Cytotherapy 12:783-91. 2010
    ..The aim was to investigate the therapeutic effect of granulocyte-colony-stimulating factor (G-CSF) administration following implantation of autologous bone marrow mononuclear cells (BM MNC) for patients with lower limb ischemia...
  89. ncbi Special characteristics of culturing mature human bladder smooth muscle cells on human amniotic membrane as a suitable matrix
    Farzaneh Sharifiaghdas
    Department of Urology, Shahid Labbafinejad Medical Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
    Urol J 6:283-8. 2009
    ..Our aim was to evaluate the natural behavior, growth pattern, morphology, and specific features of human bladder smooth muscle cells (HBSMCs) on two different matrixes, including human amniotic membrane (HAM) and collagen...
  90. ncbi Effect of basic fibroblast growth factor on cardiomyocyte differentiation from mouse embryonic stem cells
    Shiva Khezri
    Department of Stem Cells, Royan Institute, Tehran, Iran
    Saudi Med J 28:181-6. 2007
    ..To investigate the effect of basic fibroblast growth factor (bFGF) on the differentiation of embryonic stem cells (ESCs) into early cardiomyocytes...
  91. doi Intraepidermal injection of dissociated epidermal cell suspension improves vitiligo
    Laleh Khodadadi
    Department of Regenerative Medicine, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
    Arch Dermatol Res 302:593-9. 2010
    ..5% of the cells had melanocyte morphology. HMB-45 positive cells were observed after cell transplantation. This method is an effective, simple and safe therapeutic option for stable vitiligo lesions...
  92. doi Progress and promise towards safe induced pluripotent stem cells for therapy
    Ali Seifinejad
    Department of Stem Cells and Developmental Biology, Royan Institute for Stem Cell Biology and Technology, ACECR, Tehran, Iran
    Stem Cell Rev 6:297-306. 2010
    ..Here, we highlight the potential and challenges of iPSC research and review advances in reprogramming methods that have rapidly moved the field closer to realizing the goal of generating safe iPSCs for transplantation...
  93. ncbi Safety analysis and improved cardiac function following local autologous transplantation of CD133(+) enriched bone marrow cells after myocardial infarction
    Hossein Ahmadi
    Tehran Heart Center, Tehran University of Medical Sciences, Tehran, I R Iran
    Curr Neurovasc Res 4:153-60. 2007
    ..Moreover, these cells might restore tissue viability and improve perfusion of the infarcted myocardium, suggesting that they may induce myogenesis as well as angiogenesis...