Sarah Snykers

Summary

Affiliation: Vrije Universiteit Brussel
Country: Belgium

Publications

  1. pmc Chromatin remodeling agent trichostatin A: a key-factor in the hepatic differentiation of human mesenchymal stem cells derived of adult bone marrow
    Sarah Snykers
    Dept Toxicology, Vrije Universiteit Brussel, Laarbeeklaan 103, B 1090, Brussels, Belgium
    BMC Dev Biol 7:24. 2007
  2. ncbi Sequential exposure to cytokines reflecting embryogenesis: the key for in vitro differentiation of adult bone marrow stem cells into functional hepatocyte-like cells
    Sarah Snykers
    Department of Toxicology, Vrije Universiteit Brussel, B 1090 Brussels, Belgium
    Toxicol Sci 94:330-41; discussion 235-9. 2006
  3. ncbi Differentiation of neonatal rat epithelial cells from biliary origin into immature hepatic cells by sequential exposure to hepatogenic cytokines and growth factors reflecting liver development
    Sarah Snykers
    Department of Toxicology, Vrije Universiteit Brussel VUB, Laarbeeklaan 103, B 1090 Brussels, Belgium
    Toxicol In Vitro 21:1325-31. 2007
  4. pmc In vitro differentiation of embryonic and adult stem cells into hepatocytes: state of the art
    Sarah Snykers
    Department of Toxicology, Vrije Universiteit Brussel, Belgium
    Stem Cells 27:577-605. 2009
  5. doi Role of epigenetics in liver-specific gene transcription, hepatocyte differentiation and stem cell reprogrammation
    Sarah Snykers
    Department of Toxicology, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium
    J Hepatol 51:187-211. 2009
  6. doi Hepatic differentiation of mesenchymal stem cells: in vitro strategies
    Sarah Snykers
    Department of Toxicology, Vrije Universiteit Brussel, Brussels, Belgium
    Methods Mol Biol 698:305-14. 2011
  7. ncbi Differential role of epigenetic modulators in malignant and normal stem cells: a novel tool in preclinical in vitro toxicology and clinical therapy
    Sarah Snykers
    Department of Toxicology, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium
    Arch Toxicol 81:533-44. 2007
  8. ncbi The novel histone deacetylase inhibitor 4-Me2N-BAVAH differentially affects cell junctions between primary hepatocytes
    Mathieu Vinken
    Department of Toxicology, Vrije Universiteit Brussel, Laarbeeklaan 103, B 1090 Brussels, Belgium
    Toxicology 236:92-102. 2007
  9. doi Preservation of hepatocellular functionality in cultures of primary rat hepatocytes upon exposure to 4-Me2N-BAVAH, a hydroxamate-based HDAC-inhibitor
    Tom Henkens
    Department of Toxicology, Vrije Universiteit Brussel, Brussels, Belgium
    Toxicol In Vitro 25:100-9. 2011
  10. ncbi Molecular mechanisms underlying the dedifferentiation process of isolated hepatocytes and their cultures
    Greetje Elaut
    Department of Toxicology, Vrije Universiteit Brussel, Laarbeeklaan 103, B 1090 Brussels, Belgium Tamara
    Curr Drug Metab 7:629-60. 2006

Collaborators

Detail Information

Publications23

  1. pmc Chromatin remodeling agent trichostatin A: a key-factor in the hepatic differentiation of human mesenchymal stem cells derived of adult bone marrow
    Sarah Snykers
    Dept Toxicology, Vrije Universiteit Brussel, Laarbeeklaan 103, B 1090, Brussels, Belgium
    BMC Dev Biol 7:24. 2007
    ..The capability of human mesenchymal stem cells (hMSC) derived of adult bone marrow to undergo in vitro hepatic differentiation was investigated...
  2. ncbi Sequential exposure to cytokines reflecting embryogenesis: the key for in vitro differentiation of adult bone marrow stem cells into functional hepatocyte-like cells
    Sarah Snykers
    Department of Toxicology, Vrije Universiteit Brussel, B 1090 Brussels, Belgium
    Toxicol Sci 94:330-41; discussion 235-9. 2006
    ....
  3. ncbi Differentiation of neonatal rat epithelial cells from biliary origin into immature hepatic cells by sequential exposure to hepatogenic cytokines and growth factors reflecting liver development
    Sarah Snykers
    Department of Toxicology, Vrije Universiteit Brussel VUB, Laarbeeklaan 103, B 1090 Brussels, Belgium
    Toxicol In Vitro 21:1325-31. 2007
    ..The robustness of the sequential conditions, developed before for hepatic 'transdifferentiation' of rat bone marrow stem cells (rBMSC), was hereby confirmed...
  4. pmc In vitro differentiation of embryonic and adult stem cells into hepatocytes: state of the art
    Sarah Snykers
    Department of Toxicology, Vrije Universiteit Brussel, Belgium
    Stem Cells 27:577-605. 2009
    ..In addition, some recommendations are proposed to standardize, optimize, and enrich the in vitro production of hepatocyte-like cells out of stem/progenitor cells...
  5. doi Role of epigenetics in liver-specific gene transcription, hepatocyte differentiation and stem cell reprogrammation
    Sarah Snykers
    Department of Toxicology, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium
    J Hepatol 51:187-211. 2009
    ..Special attention is paid to their role in directing hepatic differentiation of primary hepatocytes and stem cells in vitro...
  6. doi Hepatic differentiation of mesenchymal stem cells: in vitro strategies
    Sarah Snykers
    Department of Toxicology, Vrije Universiteit Brussel, Brussels, Belgium
    Methods Mol Biol 698:305-14. 2011
    ..In particular, we provide a detailed modus operandi to measure the inducible cytochrome P450 (CYP)-dependent activity of MSC-derived hepatocyte-like cells...
  7. ncbi Differential role of epigenetic modulators in malignant and normal stem cells: a novel tool in preclinical in vitro toxicology and clinical therapy
    Sarah Snykers
    Department of Toxicology, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium
    Arch Toxicol 81:533-44. 2007
    ....
  8. ncbi The novel histone deacetylase inhibitor 4-Me2N-BAVAH differentially affects cell junctions between primary hepatocytes
    Mathieu Vinken
    Department of Toxicology, Vrije Universiteit Brussel, Laarbeeklaan 103, B 1090 Brussels, Belgium
    Toxicology 236:92-102. 2007
    ....
  9. doi Preservation of hepatocellular functionality in cultures of primary rat hepatocytes upon exposure to 4-Me2N-BAVAH, a hydroxamate-based HDAC-inhibitor
    Tom Henkens
    Department of Toxicology, Vrije Universiteit Brussel, Brussels, Belgium
    Toxicol In Vitro 25:100-9. 2011
    ..In conclusion, histone deacetylase inhibitors prove to be efficient agents for better maintaining a differentiated hepatic phenotype in rat hepatocyte cultures...
  10. ncbi Molecular mechanisms underlying the dedifferentiation process of isolated hepatocytes and their cultures
    Greetje Elaut
    Department of Toxicology, Vrije Universiteit Brussel, Laarbeeklaan 103, B 1090 Brussels, Belgium Tamara
    Curr Drug Metab 7:629-60. 2006
    ..In addition, identification of the conditions needed for the full in vitro maturation of hepatic progenitor cells to quiescent, functional hepatocyte-like cells opens promising perspectives...
  11. doi Characterization and hepatic differentiation of skin-derived precursors from adult foreskin by sequential exposure to hepatogenic cytokines and growth factors reflecting liver development
    Joery De Kock
    Department of Toxicology, Vrije Universiteit Brussel VUB, Laarbeeklaan 103, B 1090 Brussels, Belgium
    Toxicol In Vitro 23:1522-7. 2009
    ..In conclusion, these data show for the first time that hSKP are capable to "trans" differentiate into hepatocyte-like cells upon mimicking the in vivo micro-environment of developing hepatocytes in vitro...
  12. ncbi Differential effects of histone deacetylase inhibitors in tumor and normal cells-what is the toxicological relevance?
    Peggy Papeleu
    Department of Toxicology, Vrije Universiteit Brussel, Brussels, Belgium
    Crit Rev Toxicol 35:363-78. 2005
    ..This will not only reduce the risk for harmful exposure of patients but also save time and money...
  13. ncbi Hepatocytes in suspension
    Greetje Elaut
    Department of Toxicology, Vrije Universiteit Brussel, Belgium
    Methods Mol Biol 320:255-63. 2006
    ..We provide general recommendations for the appropriate use of hepatocytes in suspension for pharmaco-toxicological studies. We also provide protocols for the cryopreservation of freshly isolated hepatocytes and their handling on thawing...
  14. ncbi Involvement of cell junctions in hepatocyte culture functionality
    Mathieu Vinken
    Department of Toxicology, Vrije Universiteit Brussel VUB, Brussels, Belgium
    Crit Rev Toxicol 36:299-318. 2006
    ..We further describe the actual strategies to regain and maintain cell junctions in these in vitro systems over the long-term...
  15. doi Evaluation of the multipotent character of human adipose tissue-derived stem cells isolated by Ficoll gradient centrifugation and red blood cell lysis treatment
    Feras Al Battah
    Department of Toxicology, Center for Pharmaceutical Research, Vrije Universiteit Brussel VUB, Laarbeeklaan 103, B 1090 Brussels, Belgium
    Toxicol In Vitro 25:1224-30. 2011
    ..In summary, our data suggest that the choice of the most efficient isolation procedure of hADSC depends on the differentiated cell type ultimately required...
  16. ncbi Rat hepatocyte cultures: collagen gel sandwich and immobilization cultures
    Mathieu Vinken
    Department of Toxicology, Vrije Universiteit Brussel, Belgium
    Methods Mol Biol 320:247-54. 2006
    ..We describe how to set up both types of organotypical hepatocyte culture systems. Besides a detailed protocol, we give some practical tips, taken from our own experience with long-term hepatocyte culture...
  17. ncbi Rat hepatocyte cultures: conventional monolayer and cocultures with rat liver epithelial cells
    Tom Henkens
    Department of Toxicology, Vrije Universiteit Brussel, Belgium
    Methods Mol Biol 320:239-46. 2006
    ..In addition, comments derived from our own experience are given for successfully culturing primary hepatocytes...
  18. doi Evaluation of the multipotent character of human foreskin-derived precursor cells
    Joery De Kock
    Dept Toxicology, Center for Pharmaceutical Research, Vrije Universiteit Brussel VUB, Laarbeeklaan 103, B 1090 Brussels, Belgium
    Toxicol In Vitro 25:1191-202. 2011
    ..In addition, we demonstrate here for the first time that, in a suitable keratinocyte stimulating micro-environment, hSKP can generate keratinocyte-like progeny in vitro...
  19. ncbi Isolation of rat hepatocytes
    Peggy Papeleu
    Department of Toxicology, Vrije Universiteit Brussel, Belgium
    Methods Mol Biol 320:229-37. 2006
    ..This approach allows inhibition of cell-cycle reentry during hepatocyte isolation, a process known to underlie the dedifferentiation process of cultured hepatocytes...
  20. ncbi Connexins and their channels in cell growth and cell death
    Mathieu Vinken
    Department of Toxicology, Vrije Universiteit Brussel VUB, Laarbeeklaan 103, B 1090 Brussels, Belgium
    Cell Signal 18:592-600. 2006
    ..We also briefly discuss the role of gap junctional intercellular communication in carcinogenesis and the potential use of connexins as tools for cancer therapy...
  21. doi DNA methyltransferase 3a expression decreases during apoptosis in primary cultures of hepatocytes
    Mathieu Vinken
    Department of Toxicology, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Laarbeeklaan 103, B 1090 Brussels, Belgium
    Toxicol In Vitro 24:445-51. 2010
    ..This finding further substantiates the existence of an epigenetic signature of apoptosis...
  22. doi Successful isolation of liver progenitor cells by aldehyde dehydrogenase activity in naïve mice
    Laurent Dollé
    Liver Cell Biology Laboratory, Vrije Universiteit Brussels, Brussels, Belgium
    Hepatology 55:540-52. 2012
    ..Finally, we showed that the isolation and differentiation toward hepatocyte-like cells of LPCs with high ALDH activity is also successfully applicable to human liver samples...
  23. ncbi Isolation of rat bone marrow stem cells
    Sarah Snykers
    Department of Toxicology, Vrije Universiteit Brussel, Belgium
    Methods Mol Biol 320:265-72. 2006
    ..We describe a protocol for isolation of mononuclear cells from adult rat bone marrow...