Mathieu Vinken

Summary

Affiliation: Vrije Universiteit Brussel
Country: Belgium

Publications

  1. ncbi request reprint Connexins and their channels in cell growth and cell death
    Mathieu Vinken
    Department of Toxicology, Vrije Universiteit Brussel VUB, Laarbeeklaan 103, B 1090 Brussels, Belgium
    Cell Signal 18:592-600. 2006
  2. doi request reprint Epigenetic regulation of gap junctional intercellular communication: more than a way to keep cells quiet?
    Mathieu Vinken
    Department of Toxicology, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Brussels, Belgium
    Biochim Biophys Acta 1795:53-61. 2009
  3. doi request reprint Gap junctional intercellular communication as a target for liver toxicity and carcinogenicity
    Mathieu Vinken
    Department of Toxicology, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Brussels, Belgium
    Crit Rev Biochem Mol Biol 44:201-22. 2009
  4. doi request reprint Connexin32 hemichannels contribute to the apoptotic-to-necrotic transition during Fas-mediated hepatocyte cell death
    Mathieu Vinken
    Department of Toxicology, Vrije Universiteit Brussel, Belgium
    Cell Mol Life Sci 67:907-18. 2010
  5. doi request reprint Introduction: connexins, pannexins and their channels as gatekeepers of organ physiology.
    Mathieu Vinken
    Department of In Vitro Toxicology and Dermato Cosmetology, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090, Brussels, Belgium
    Cell Mol Life Sci 72:2775-8. 2015
  6. doi request reprint Adverse outcome pathways: a concise introduction for toxicologists
    Mathieu Vinken
    Department of In Vitro Toxicology and Dermato Cosmetology, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090, Brussels, Belgium
    Arch Toxicol 91:3697-3707. 2017
  7. pmc In vitro testing of basal cytotoxicity: Establishment of an adverse outcome pathway from chemical insult to cell death
    Mathieu Vinken
    Department of In Vitro Toxicology and Dermato Cosmetology, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium Electronic address
    Toxicol In Vitro 39:104-110. 2017
  8. pmc Adverse Outcome Pathways as Tools to Assess Drug-Induced Toxicity
    Mathieu Vinken
    Department of In Vitro Toxicology and Dermato Cosmetology, Pharmaceutical Institute, Vrije Universiteit Brussel, Laarbeeklaan 103, Brussels Jette, 1090, Belgium
    Methods Mol Biol 1425:325-37. 2016
  9. pmc Regulation of connexin signaling by the epigenetic machinery
    Mathieu Vinken
    Vrije Universiteit Brussel, Department of In Vitro Toxicology and Dermato Cosmetology, Building G, Room G226, Laarbeeklaan 103, B 1090 Brussels, Belgium Electronic address
    Biochim Biophys Acta 1859:262-8. 2016
  10. pmc Establishment and Characterization of an In Vitro Model of Fas-Mediated Hepatocyte Cell Death
    Mathieu Vinken
    Department of In Vitro Toxicology and Dermato Cosmetology, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090, Brussels, Belgium
    Methods Mol Biol 1250:95-103. 2015

Detail Information

Publications81

  1. ncbi request reprint Connexins and their channels in cell growth and cell death
    Mathieu Vinken
    Department of Toxicology, Vrije Universiteit Brussel VUB, Laarbeeklaan 103, B 1090 Brussels, Belgium
    Cell Signal 18:592-600. 2006
    ..We also briefly discuss the role of gap junctional intercellular communication in carcinogenesis and the potential use of connexins as tools for cancer therapy...
  2. doi request reprint Epigenetic regulation of gap junctional intercellular communication: more than a way to keep cells quiet?
    Mathieu Vinken
    Department of Toxicology, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Brussels, Belgium
    Biochim Biophys Acta 1795:53-61. 2009
    ..Besides an updated theoretical background concerning gap junctions and epigenetic phenomena, we provide an in-depth overview of their interrelationship and we demonstrate the clinical relevance of the topic...
  3. doi request reprint Gap junctional intercellular communication as a target for liver toxicity and carcinogenicity
    Mathieu Vinken
    Department of Toxicology, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Brussels, Belgium
    Crit Rev Biochem Mol Biol 44:201-22. 2009
    ....
  4. doi request reprint Connexin32 hemichannels contribute to the apoptotic-to-necrotic transition during Fas-mediated hepatocyte cell death
    Mathieu Vinken
    Department of Toxicology, Vrije Universiteit Brussel, Belgium
    Cell Mol Life Sci 67:907-18. 2010
    ..We conclude that connexin32 hemichannels facilitate the apoptotic-to-necrotic transition, which typically occurs in the final stage of hepatocellular apoptosis...
  5. doi request reprint Introduction: connexins, pannexins and their channels as gatekeepers of organ physiology.
    Mathieu Vinken
    Department of In Vitro Toxicology and Dermato Cosmetology, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090, Brussels, Belgium
    Cell Mol Life Sci 72:2775-8. 2015
  6. doi request reprint Adverse outcome pathways: a concise introduction for toxicologists
    Mathieu Vinken
    Department of In Vitro Toxicology and Dermato Cosmetology, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090, Brussels, Belgium
    Arch Toxicol 91:3697-3707. 2017
    ..The present paper provides a synopsis of the main principles related to the AOP framework for the toxicologist less familiar with this area, followed by two case studies relevant for human toxicology and ecotoxicology...
  7. pmc In vitro testing of basal cytotoxicity: Establishment of an adverse outcome pathway from chemical insult to cell death
    Mathieu Vinken
    Department of In Vitro Toxicology and Dermato Cosmetology, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium Electronic address
    Toxicol In Vitro 39:104-110. 2017
    ..The suggested strategy to consider in vitro basal cytotoxicity as a first step in evaluating the toxicity of new chemical entities can be placed in a tiered strategy that could be continued by evaluating more specific types of toxicity...
  8. pmc Adverse Outcome Pathways as Tools to Assess Drug-Induced Toxicity
    Mathieu Vinken
    Department of In Vitro Toxicology and Dermato Cosmetology, Pharmaceutical Institute, Vrije Universiteit Brussel, Laarbeeklaan 103, Brussels Jette, 1090, Belgium
    Methods Mol Biol 1425:325-37. 2016
    ....
  9. pmc Regulation of connexin signaling by the epigenetic machinery
    Mathieu Vinken
    Vrije Universiteit Brussel, Department of In Vitro Toxicology and Dermato Cosmetology, Building G, Room G226, Laarbeeklaan 103, B 1090 Brussels, Belgium Electronic address
    Biochim Biophys Acta 1859:262-8. 2016
    ..This paper provides an overview of the role of major determinants of the epigenome, including DNA methylation, histone acetylation and microRNA species, in connexin expression. ..
  10. pmc Establishment and Characterization of an In Vitro Model of Fas-Mediated Hepatocyte Cell Death
    Mathieu Vinken
    Department of In Vitro Toxicology and Dermato Cosmetology, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090, Brussels, Belgium
    Methods Mol Biol 1250:95-103. 2015
    ..This experimental system allows the study of the entire course of Fas-mediated hepatocellular cell death, going from early apoptosis to secondary necrosis, and hence can serve a broad range of in vitro pharmaco-toxicological purposes. ..
  11. pmc Primary hepatocytes and their cultures in liver apoptosis research
    Mathieu Vinken
    Department of Toxicology FAFY, Faculty of Medicine and Pharmacy, Center for Pharmaceutical Research CePhaR, Vrije Universiteit Brussel VUB, Laarbeeklaan 103, 1090, Brussels, Belgium
    Arch Toxicol 88:199-212. 2014
    ..In addition, currently applied approaches to experimentally induce controlled apoptosis in this in vitro setting for mechanistic research purposes and thereby its detection using relevant biomarkers are reviewed. ..
  12. doi request reprint Non-channel functions of connexins in cell growth and cell death
    Mathieu Vinken
    Department of Toxicology Center for Pharmaceutical Research, Vrije Universiteit Brussel, Brussels, Belgium
    Biochim Biophys Acta 1818:2002-8. 2012
    ..This article is part of a Special Issue entitled: The Communicating junctions, composition, structure and characteristics...
  13. doi request reprint The adverse outcome pathway concept: a pragmatic tool in toxicology
    Mathieu Vinken
    Department of Toxicology, Center for Pharmaceutical Research CePhaR, Vrije Universiteit Brussel VUB, Belgium Electronic address
    Toxicology 312:158-65. 2013
    ....
  14. ncbi request reprint Drug-induced liver injury: mechanisms, types and biomarkers
    M Vinken
    Department of Toxicology, Center for Pharmaceutical Research, Vrije Universiteit Brussel, Laarbeeklaan 103, B 1090 Brussels, Belgium
    Curr Med Chem 20:3011-21. 2013
    ..These new concepts and recent developments in the field of drug-induced liver injury are revised in the current paper. ..
  15. doi request reprint Proteomic and metabolomic responses to connexin43 silencing in primary hepatocyte cultures
    Mathieu Vinken
    Department of Toxicology, Faculty of Medicine and Pharmacy, Center for Pharmaceutical Research, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090, Brussel, Belgium
    Arch Toxicol 87:883-94. 2013
    ....
  16. doi request reprint Primary hepatocyte cultures as in vitro tools for toxicity testing: quo vadis?
    Mathieu Vinken
    Department of Toxicology, Center for Pharmaceutical Research, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Laarbeeklaan 103, B 1090 Brussels, Belgium
    Toxicol In Vitro 26:541-4. 2012
    ..Such experimental system is urgently needed, especially in the light of the stringent European legislative modifications that are currently encountered by the pharmaceutical, chemical and, particularly, the cosmetic industry...
  17. doi request reprint Inhibition of gap junctional intercellular communication by toxic metals
    Mathieu Vinken
    Department of Toxicology, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Laarbeeklaan 103, B 1090 Brussels, Belgium
    Chem Res Toxicol 23:1862-7. 2010
    ....
  18. doi request reprint Modifications in connexin expression in liver development and cancer
    Mathieu Vinken
    Department of Toxicology, Center for Pharmaceutical Research, Vrije Universiteit Brussel VUB, Brussel, Belgium
    Cell Commun Adhes 19:55-62. 2012
    ..Abnormalities of connexin production are observed in a number of pathological conditions, such as during liver cancer. This article provides an overview of these processes with emphasis on the underlying molecular mechanisms...
  19. doi request reprint Gap junctions and non-neoplastic liver disease
    Mathieu Vinken
    Department of Toxicology, Center for Pharmaceutical Research, Vrije Universiteit Brussel, Laarbeeklaan 103, B 1090 Brussels, Belgium
    J Hepatol 57:655-62. 2012
    ..The use of connexins as biomarkers and therapeutic targets in liver disease is also illustrated...
  20. doi request reprint Screening of repeated dose toxicity data present in SCC(NF)P/SCCS safety evaluations of cosmetic ingredients
    Mathieu Vinken
    Department of Toxicology, Dermato Cosmetology and Pharmacognosy, Center for Pharmaceutical Research, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium
    Arch Toxicol 86:405-12. 2012
    ....
  21. doi request reprint Characterization of spontaneous cell death in monolayer cultures of primary hepatocytes
    Mathieu Vinken
    Department of Toxicology, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium
    Arch Toxicol 85:1589-96. 2011
    ....
  22. doi request reprint Connexin43 signaling contributes to spontaneous apoptosis in cultures of primary hepatocytes
    Mathieu Vinken
    Department of Toxicology Center for Pharmaceutical Research, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, B 1090 Brussels, Belgium
    Toxicol Sci 125:175-86. 2012
    ..Collectively, these data show that Cx43 signaling actively contributes to the occurrence of spontaneous apoptosis in cultures of primary hepatocytes...
  23. doi request reprint Connexins: sensors and regulators of cell cycling
    Mathieu Vinken
    Department of Toxicology, Vrije Universiteit Brussel, Laarbeeklaan 103, B 1090 Brussels, Belgium
    Biochim Biophys Acta 1815:13-25. 2011
    ..In the current paper, these multifaceted aspects of connexin-related signalling in cell cycling are reviewed...
  24. doi request reprint DNA methyltransferase 3a expression decreases during apoptosis in primary cultures of hepatocytes
    Mathieu Vinken
    Department of Toxicology, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Laarbeeklaan 103, B 1090 Brussels, Belgium
    Toxicol In Vitro 24:445-51. 2010
    ..This finding further substantiates the existence of an epigenetic signature of apoptosis...
  25. ncbi request reprint Biochemical characterisation of an in vitro model of hepatocellular apoptotic cell death
    Mathieu Vinken
    Department of Toxicology, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Brussels, Belgium
    Altern Lab Anim 37:209-18. 2009
    ..This experimental system can serve a broad range of in vitro pharmaco-toxicological purposes, thereby directly assisting in the reduction of animal experimentation...
  26. doi request reprint The carcinoGENOMICS project: critical selection of model compounds for the development of omics-based in vitro carcinogenicity screening assays
    Mathieu Vinken
    Department of Toxicology, Vrije Universiteit Brussel VUB, Laarbeeklaan 103, B 1090 Brussels, Belgium
    Mutat Res 659:202-10. 2008
    ..Since selecting an appropriate set of chemicals is a frequent impediment in the early stages of similar research projects, the information provided in this paper might be extremely valuable...
  27. ncbi request reprint Biology and pathobiology of gap junctional channels in hepatocytes
    Mathieu Vinken
    Department of Toxicology, Vrije Universiteit Brussel, Laarbeeklaan 103, B 1090 Brussels, Belgium
    Hepatology 47:1077-88. 2008
    ..Finally, a number of directions for future liver gap junctional channel research are proposed, and the up-regulation of gap junctional channel activity as a novel strategy in (liver) cancer therapy is illustrated...
  28. ncbi request reprint The novel histone deacetylase inhibitor 4-Me2N-BAVAH differentially affects cell junctions between primary hepatocytes
    Mathieu Vinken
    Department of Toxicology, Vrije Universiteit Brussel, Laarbeeklaan 103, B 1090 Brussels, Belgium
    Toxicology 236:92-102. 2007
    ....
  29. ncbi request reprint Involvement of cell junctions in hepatocyte culture functionality
    Mathieu Vinken
    Department of Toxicology, Vrije Universiteit Brussel VUB, Brussels, Belgium
    Crit Rev Toxicol 36:299-318. 2006
    ..We further describe the actual strategies to regain and maintain cell junctions in these in vitro systems over the long-term...
  30. ncbi request reprint Histone deacetylase inhibitors as potent modulators of cellular contacts
    Mathieu Vinken
    Department of Toxicology, Vrije Universiteit Brussel VUB, Laarbeeklaan 103, B 1090 Brussels, Belgium
    Curr Drug Targets 7:773-87. 2006
    ..Besides an updated theoretical basis, we also exemplify its actual relevance in cancer therapy...
  31. ncbi request reprint Trichostatin a enhances gap junctional intercellular communication in primary cultures of adult rat hepatocytes
    Mathieu Vinken
    Department of Toxicology, Vrije Universiteit Brussel, B 1090 Brussels, Belgium
    Toxicol Sci 91:484-92. 2006
    ..TSA enhances GJIC between primary cultured rat hepatocytes, an interesting finding supporting its use to further optimize liver-based in vitro models for pharmacotoxicological purposes...
  32. doi request reprint Differential effects of hydroxamate histone deacetylase inhibitors on cellular functionality and gap junctions in primary cultures of mitogen-stimulated hepatocytes
    Tom Henkens
    Department of Toxicology, Vrije Universiteit Brussel VUB, Laarbeeklaan 103, B 1090 Brussels, Belgium
    Toxicol Lett 178:37-43. 2008
    ..These data provide new insight into the biological impact of HDAC-inhibitors on the homeostatic balance in hepatocytes, being major executors of xenobiotic biotransformation and primary targets of drug-induced toxicity...
  33. doi request reprint Role of epigenetics in liver-specific gene transcription, hepatocyte differentiation and stem cell reprogrammation
    Sarah Snykers
    Department of Toxicology, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium
    J Hepatol 51:187-211. 2009
    ..Special attention is paid to their role in directing hepatic differentiation of primary hepatocytes and stem cells in vitro...
  34. ncbi request reprint Trichostatin A, a critical factor in maintaining the functional differentiation of primary cultured rat hepatocytes
    Tom Henkens
    Department of Toxicology, Pharmaceutical Institute, Vrije Universiteit Brussel, Laarbeeklaan 103, B 1090 Brussels, Belgium
    Toxicol Appl Pharmacol 218:64-71. 2007
    ..It is suggested that the effects of TSA on CYP gene expression are mediated via controlling the expression of LETFs...
  35. doi request reprint Comparison of genotoxicant-modified transcriptomic responses in conventional and epigenetically stabilized primary rat hepatocytes with in vivo rat liver data
    Tatyana Y Doktorova
    Department of Toxicology, Center for Pharmaceutical Research CePhaR, Vrije Universiteit Brussel VUB, Laarbeeklaan 103, Brussels, Belgium
    Arch Toxicol 86:1703-15. 2012
    ....
  36. doi request reprint Connexin hemichannel inhibition reduces acetaminophen-induced liver injury in mice
    Michael Maes
    Department of In Vitro Toxicology and Dermato Cosmetology, Vrije Universiteit Brussel, Brussels, Belgium Electronic address
    Toxicol Lett 278:30-37. 2017
    ..This study shows for the first time a role for connexin hemichannels in acetaminophen-induced acute liver failure...
  37. doi request reprint Comparison of hepatocarcinogen-induced gene expression profiles in conventional primary rat hepatocytes with in vivo rat liver
    Tatyana Y Doktorova
    Department of Toxicology, Center for Pharmaceutical Research, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium
    Arch Toxicol 86:1399-411. 2012
    ..This study contributes to the further optimization of toxicogenomics predictive tools when applied in in vitro settings...
  38. doi request reprint Preservation of hepatocellular functionality in cultures of primary rat hepatocytes upon exposure to 4-Me2N-BAVAH, a hydroxamate-based HDAC-inhibitor
    Tom Henkens
    Department of Toxicology, Vrije Universiteit Brussel, Brussels, Belgium
    Toxicol In Vitro 25:100-9. 2011
    ..In conclusion, histone deacetylase inhibitors prove to be efficient agents for better maintaining a differentiated hepatic phenotype in rat hepatocyte cultures...
  39. pmc Experimental models of liver fibrosis
    Sara Crespo Yanguas
    Department of In Vitro Toxicology and Dermato Cosmetology, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090, Brussels, Belgium
    Arch Toxicol 90:1025-48. 2016
    ..The present paper provides a state-of-the-art overview of in vivo and in vitro models used in the field of experimental liver fibrosis research. ..
  40. doi request reprint Synergetic effects of DNA demethylation and histone deacetylase inhibition in primary rat hepatocytes
    Joanna Edyta Fraczek
    Department of Toxicology, Center for Pharmaceutical Research, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium
    Invest New Drugs 30:1715-24. 2012
    ..The functionality of the hepatocytes was evidenced by an increased expression of the phase I biotransformation enzyme cytochrome P 450 (CYP) 1A1 and albumin secretion capacity when both agents were used in combination...
  41. ncbi request reprint Molecular mechanisms underlying the dedifferentiation process of isolated hepatocytes and their cultures
    Greetje Elaut
    Department of Toxicology, Vrije Universiteit Brussel, Laarbeeklaan 103, B 1090 Brussels, Belgium Tamara
    Curr Drug Metab 7:629-60. 2006
    ..In addition, identification of the conditions needed for the full in vitro maturation of hepatic progenitor cells to quiescent, functional hepatocyte-like cells opens promising perspectives...
  42. ncbi request reprint Differential effects of histone deacetylase inhibitors in tumor and normal cells-what is the toxicological relevance?
    Peggy Papeleu
    Department of Toxicology, Vrije Universiteit Brussel, Brussels, Belgium
    Crit Rev Toxicol 35:363-78. 2005
    ..This will not only reduce the risk for harmful exposure of patients but also save time and money...
  43. pmc Chromatin remodeling agent trichostatin A: a key-factor in the hepatic differentiation of human mesenchymal stem cells derived of adult bone marrow
    Sarah Snykers
    Dept Toxicology, Vrije Universiteit Brussel, Laarbeeklaan 103, B 1090, Brussels, Belgium
    BMC Dev Biol 7:24. 2007
    ..The capability of human mesenchymal stem cells (hMSC) derived of adult bone marrow to undergo in vitro hepatic differentiation was investigated...
  44. ncbi request reprint Modulation of CYP1A1 and CYP2B1 expression upon cell cycle progression in cultures of primary rat hepatocytes
    Tom Henkens
    Department of Toxicology, Pharmaceutical Institute, Vrije Universiteit Brussel, Laarbeeklaan 103, B 1090 Brussels, Belgium
    Toxicol In Vitro 21:1253-7. 2007
    ..Therefore, in order to maintain primary hepatocytes functional in culture, cell cycle inhibition must be achieved...
  45. pmc Involvement of connexin43 in acetaminophen-induced liver injury
    Michael Maes
    Department of In Vitro Toxicology and Dermato Cosmetology, Vrije Universiteit Brussel, Brussels, Belgium
    Biochim Biophys Acta 1862:1111-21. 2016
    ..In the present study, the involvement of connexin26, connexin32 and connexin43, the building blocks of liver gap junctions, was investigated in acetaminophen-induced hepatotoxicity...
  46. doi request reprint Transcriptomic responses generated by hepatocarcinogens in a battery of liver-based in vitro models
    Tatyana Y Doktorova
    Department of Toxicology, Center for Pharmaceutical Research, Vrije Universiteit Brussel, Laarbeeklaan 103, B 1090 Brussels, Belgium
    Carcinogenesis 34:1393-402. 2013
    ....
  47. ncbi request reprint Hepatocytes in suspension
    Greetje Elaut
    Department of Toxicology, Vrije Universiteit Brussel, Belgium
    Methods Mol Biol 320:255-63. 2006
    ..We provide general recommendations for the appropriate use of hepatocytes in suspension for pharmaco-toxicological studies. We also provide protocols for the cryopreservation of freshly isolated hepatocytes and their handling on thawing...
  48. doi request reprint Opportunities for an alternative integrating testing strategy for carcinogen hazard assessment?
    Tatyana Y Doktorova
    Department of Toxicology, Center for Pharmaceutical Research, Vrije Universiteit Brussel VUB, Laarbeeklaan, Brussels, Belgium
    Crit Rev Toxicol 42:91-106. 2012
    ....
  49. pmc Models and methods for in vitro testing of hepatic gap junctional communication
    Michael Maes
    Department of In Vitro Toxicology and Dermato Cosmetology, Vrije Universiteit Brussel, Laarbeeklaan 103, B 1090 Brussels, Belgium Electronic address
    Toxicol In Vitro 30:569-77. 2015
    ..Both these models and methods are discussed in the current paper, thereby focusing on connexin32-based gap junctions. ..
  50. doi request reprint Way forward in case of a false positive in vitro genotoxicity result for a cosmetic substance?
    Tatyana Y Doktorova
    Department of Toxicology, Center for Pharmaceutical Research, Vrije Universiteit Brussel, 1090 Brussels, Belgium Electronic address
    Toxicol In Vitro 28:54-9. 2014
    ..As such a substantial number of cosmetic compounds wrongly identified as genotoxicants could be saved for the future. ..
  51. pmc Inhibition of pannexin1 channels alleviates acetaminophen-induced hepatotoxicity
    Michael Maes
    Department of In Vitro Toxicology and Dermato Cosmetology, Vrije Universiteit Brussel, Pleinlaan 2, 1050, Brussels, Belgium
    Arch Toxicol . 2016
    ..Pannexin1 channels are important actors in liver injury triggered by acetaminophen. Inhibition of pannexin1 channel opening could represent a novel approach for the treatment of drug-induced hepatotoxicity...
  52. pmc Pannexin1 as mediator of inflammation and cell death
    Sara Crespo Yanguas
    Department of In Vitro Toxicology and Dermato Cosmetology, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Brussels, Belgium
    Biochim Biophys Acta 1864:51-61. 2017
    ..In a first part, a state-of-the-art overview of pannexin channel structure, regulation and function is provided. In a second part, the mechanisms behind their involvement in inflammation and cell death are discussed...
  53. pmc Connexins and their channels in inflammation
    Joost Willebrords
    a Department of in Vitro Toxicology and Dermato Cosmetology, Vrije Universiteit Brussel, Brussels, Belgium
    Crit Rev Biochem Mol Biol 51:413-439. 2016
    ..A better understanding of the importance of connexin signaling in inflammation may open up towards clinical perspectives...
  54. pmc Structure, Regulation and Function of Gap Junctions in Liver
    Joost Willebrords
    a Department of in Vitro Toxicology and Dermato Cosmetology, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Brussels, Belgium
    Cell Commun Adhes . 2016
    ..This paper reviews established and novel aspects regarding the architecture, control and functional relevance of liver gap junctions...
  55. ncbi request reprint Isolation of rat hepatocytes
    Peggy Papeleu
    Department of Toxicology, Vrije Universiteit Brussel, Belgium
    Methods Mol Biol 320:229-37. 2006
    ..This approach allows inhibition of cell-cycle reentry during hepatocyte isolation, a process known to underlie the dedifferentiation process of cultured hepatocytes...
  56. ncbi request reprint Rat hepatocyte cultures: conventional monolayer and cocultures with rat liver epithelial cells
    Tom Henkens
    Department of Toxicology, Vrije Universiteit Brussel, Belgium
    Methods Mol Biol 320:239-46. 2006
    ..In addition, comments derived from our own experience are given for successfully culturing primary hepatocytes...
  57. ncbi request reprint Rat hepatocyte cultures: collagen gel sandwich and immobilization cultures
    Mathieu Vinken
    Department of Toxicology, Vrije Universiteit Brussel, Belgium
    Methods Mol Biol 320:247-54. 2006
    ..We describe how to set up both types of organotypical hepatocyte culture systems. Besides a detailed protocol, we give some practical tips, taken from our own experience with long-term hepatocyte culture...
  58. doi request reprint Effect of Trichostatin A on miRNA expression in cultures of primary rat hepatocytes
    Jennifer Bolleyn
    Department of Toxicology, Center for Pharmaceutical Research, Vrije Universiteit Brussel VUB, Laarbeeklaan 103, B 1090 Brussels, Belgium
    Toxicol In Vitro 25:1173-82. 2011
    ....
  59. doi request reprint Inhibition of connexin hemichannels alleviates non-alcoholic steatohepatitis in mice
    Joost Willebrords
    Department of In Vitro Toxicology and Dermato Cosmetology, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090, Brussels, Belgium
    Sci Rep 7:8268. 2017
    ..These findings show the involvement of connexin32 and connexin43 hemichannels in non-alcoholic steatohepatitis and, simultaneously, suggest a role as potential drug targets in non-alcoholic steatohepatitis...
  60. doi request reprint Development of an adverse outcome pathway from drug-mediated bile salt export pump inhibition to cholestatic liver injury
    Mathieu Vinken
    Department of Toxicology, Center for Pharmaceutical Research, Vrije Universiteit Brussel VUB, B 1090 Brussels, Belgium
    Toxicol Sci 136:97-106. 2013
    ..The postulated AOP is expected to serve as the basis for the development of new in vitro tests and the characterization of novel biomarkers of drug-induced cholestasis. ..
  61. pmc Connexins and pannexins in liver damage
    Sara Crespo Yanguas
    Department of In Vitro Toxicology and Dermato Cosmetology, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium
    EXCLI J 15:177-86. 2016
    ..As they act both as sensors and effectors in these deleterious events connexins and pannexins could represent a set of novel clinical diagnostic biomarkers and drug targets. ..
  62. ncbi request reprint Differential role of epigenetic modulators in malignant and normal stem cells: a novel tool in preclinical in vitro toxicology and clinical therapy
    Sarah Snykers
    Department of Toxicology, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium
    Arch Toxicol 81:533-44. 2007
    ....
  63. pmc Measurement of Apoptotic and Necrotic Cell Death in Primary Hepatocyte Cultures
    Michael Maes
    Department of In Vitro Toxicology and Dermato Cosmetology, Vrije Universiteit Brussel, Laarbeeklaan 103, Brussels, 1090, Belgium
    Methods Mol Biol 1250:349-61. 2015
    ..The latter can be indirectly assessed by spectrophotometrically measuring the consumption of reduced nicotinamide adenine dinucleotide. ..
  64. doi request reprint Critical selection of reliable reference genes for gene expression study in the HepaRG cell line
    Liesbeth Ceelen
    Department of Toxicology, Faculty of Medicine and Pharmacy, Center for Pharmaceutical Research, Vrije Universiteit Brussel, Laarbeeklaan 103, Brussels, Belgium
    Biochem Pharmacol 81:1255-61. 2011
    ..This study provides a new set of reference genes that is suitable for testing whenever RT-qPCR data for HepaRG cells are generated. The most stable ones can then be selected for further normalization...
  65. doi request reprint Modulation of connexin signaling by bacterial pathogens and their toxins
    Liesbeth Ceelen
    Department of Toxicology, Centre for Pharmaceutical Research, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium
    Cell Mol Life Sci 68:3047-64. 2011
    ..Particular attention is paid to the underlying molecular mechanisms of these effects as well as to the actual biological relevance of these findings...
  66. pmc Connexin32: a mediator of acetaminophen-induced liver injury?
    Michael Maes
    a Department of in Vitro Toxicology and Dermato Cosmetology, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Brussels, Belgium
    Toxicol Mech Methods 26:88-96. 2016
    ..This could question the suitability of the currently available models and tools to investigate the role of connexin32 in acetaminophen-triggered hepatotoxicity. ..
  67. pmc Detection of Connexins in Liver Cells Using Sodium Dodecyl Sulfate Polyacrylamide Gel Electrophoresis and Immunoblot Analysis
    Joost Willebrords
    Department of In Vitro Toxicology and Dermato Cosmetology, Vrije Universiteit Brussel, Laarkbeeklaan 103, 1090, Jette, Brussel, Belgium
    Methods Mol Biol 1437:37-53. 2016
    ..The latter allows specific detection of connexins with antibodies combined with revelation through enhanced chemiluminescence. ..
  68. pmc Analysis of Liver Connexin Expression Using Reverse Transcription Quantitative Real-Time Polymerase Chain Reaction
    Michael Maes
    Department of In Vitro Toxicology and Dermato Cosmetology, Vrije Universiteit Brussel, Laarkbeeklaan 103, 1090, Jette, Brussel, Belgium
    Methods Mol Biol 1437:1-19. 2016
    ..The method includes RNA extraction and subsequent quantification, generation of complementary DNA, quantitative real-time polymerase chain reaction, and data analysis. ..
  69. pmc Roles of connexins and pannexins in digestive homeostasis
    Michael Maes
    Department of In Vitro Toxicology and Dermato Cosmetology, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090, Brussels, Belgium
    Cell Mol Life Sci 72:2809-21. 2015
    ....
  70. doi request reprint MicroRNAs as key regulators of xenobiotic biotransformation and drug response
    Jennifer Bolleyn
    Department of Toxicology, Center for Pharmaceutical Research, Vrije Universiteit Brussel VUB, Laarbeeklaan 103, 1090, Brussels, Belgium
    Arch Toxicol 89:1523-41. 2015
    ..Nuclear receptors and transcription factors, known to be involved in the transcriptional regulation of these genes, are also discussed...
  71. pmc Connexin and pannexin signaling in gastrointestinal and liver disease
    Michael Maes
    Department of In Vitro Toxicology and Dermato Cosmetology, Vrije Universiteit Brussel, Brussels, Belgium
    Transl Res 166:332-43. 2015
    ..This could open promising perspectives for the characterization of new targets and biomarkers for therapeutic and diagnostic clinical purposes in the area of gastroenterology and hepatology. ..
  72. pmc Strategies for immortalization of primary hepatocytes
    Eva Ramboer
    Department of Toxicology, Center for Pharmaceutical Research, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussel, Belgium Electronic address
    J Hepatol 61:925-43. 2014
    ..This paper discusses the current immortalization techniques and provides a state-of-the-art overview of the actually available immortalized hepatocyte-derived cell lines and their applications. ..
  73. doi request reprint Primary hepatocyte cultures as prominent in vitro tools to study hepatic drug transporters
    Eva Ramboer
    Department of Toxicology, Center for Pharmaceutical Research, Vrije Universiteit Brussel, Brussels, Belgium
    Drug Metab Rev 45:196-217. 2013
    ..In the second part, the use of PHCs to assess hepatobiliary transport and transporter-mediated interactions is outlined...
  74. pmc Immortalized Human Hepatic Cell Lines for In Vitro Testing and Research Purposes
    Eva Ramboer
    Department of In Vitro Toxicology and Dermato Cosmetology, Vrije Universiteit Brussel, Laarbeeklaan 103, Brussels, 1090, Belgium
    Methods Mol Biol 1250:53-76. 2015
    ..The present chapter describes currently applied immortalization strategies and provides an overview of the actually available immortalized human hepatic cell lines and their in vitro applications. ..
  75. pmc Experimental models of hepatotoxicity related to acute liver failure
    Michael Maes
    Department of In Vitro Toxicology and Dermato Cosmetology, Vrije Universiteit Brussel, Brussels, Belgium
    Toxicol Appl Pharmacol 290:86-97. 2016
    ..Each of these models has a number of strengths and weaknesses. This paper specifically reviews commonly used chemical in vivo and in vitro models of hepatotoxicity associated with acute liver failure. ..
  76. pmc Strategies, models and biomarkers in experimental non-alcoholic fatty liver disease research
    Joost Willebrords
    Department of In Vitro Toxicology and Dermato Cosmetology, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium Electronic address
    Prog Lipid Res 59:106-25. 2015
    ..Furthermore, the current state-of-art regarding experimental biomarkers such as epigenetic, genetic, transcriptomic, proteomic and metabonomic biomarkers will be reviewed. ..
  77. pmc Emerging roles of connexin hemichannels in gastrointestinal and liver pathophysiology
    Mathieu Vinken
    Mathieu Vinken, Tamara Vanhaecke, Vera Rogiers, Department of Toxicology, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel, Laarbeeklaan 103, Brussels, B 1090, Belgium
    World J Gastrointest Pathophysiol 1:115-7. 2010
    ..Research in this field still has a number of shortcomings, of which some are also discussed here...
  78. doi request reprint Proteomic analysis of global protein expression changes in the endothelin-1 rat model for cerebral ischemia: rescue effect of mild hypothermia
    Tine Zgavc
    Department of Pharmaceutical Chemistry and Drug Analysis, Centre for Neuroscience, Vrije Universiteit Brussel, Laarbeeklaan 103, 1090 Brussels, Belgium Electronic address
    Neurochem Int 63:379-88. 2013
    ....
  79. pmc Human skin-derived stem cells as a novel cell source for in vitro hepatotoxicity screening of pharmaceuticals
    Robim M Rodrigues
    1 Department of Toxicology, Center for Pharmaceutical Research, Faculty of Medicine and Pharmacy, Vrije Universiteit Brussel VUB, Brussels, Belgium
    Stem Cells Dev 23:44-55. 2014
    ..To the best of our knowledge, this is the first report in which human postnatal stem cells derived from skin are described as a potentially relevant cell source for in vitro hepatotoxicity testing of pharmaceutical compounds. ..
  80. doi request reprint Prolonged gene silencing in hepatoma cells and primary hepatocytes after small interfering RNA delivery with biodegradable poly(beta-amino esters)
    Roosmarijn E Vandenbroucke
    Laboratory of General Biochemistry and Physical Pharmacy, Ghent University, Ghent, Belgium
    J Gene Med 10:783-94. 2008
    ..Poly(beta-amino esters) (PbAEs) are, in contrast to many other cationic polymers evaluated in siRNA delivery, biodegradable into smaller, nontoxic molecules...
  81. ncbi request reprint Junctional structures and hepatocellular carcinoma: from the lab to the clinic?
    Mathieu Vinken
    Liver Int 28:432-4. 2008