Marcio V Ramos

Summary

Affiliation: Universidade Federal do Cear
Country: Brazil

Publications

  1. doi request reprint Peptidases and peptidase inhibitors in gut of caterpillars and in the latex of their host plants
    Marcio V Ramos
    Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Campus do Pici, Cx Postal 6033, Fortaleza, CE, CEP 60451 970, Brazil
    Planta 241:167-78. 2015
  2. pmc Insights on the phytochemical profile (cyclopeptides) and biological activities of Calotropis procera latex organic fractions
    Thiago Lustosa Jucá
    Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Av Humberto Monte, Campus do Pici, 60451 970 Fortaleza, CE, Brazil
    ScientificWorldJournal 2013:615454. 2013
  3. doi request reprint New insights into the complex mixture of latex cysteine peptidases in Calotropis procera
    M V Ramos
    Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Fortaleza, CE, Brazil
    Int J Biol Macromol 58:211-9. 2013
  4. doi request reprint Proteins derived from latex of C. procera maintain coagulation homeostasis in septic mice and exhibit thrombin- and plasmin-like activities
    Marcio V Ramos
    Departamento de Bioquimica e Biologia Molecular, Campus do Pici, bloco 907, Universidade Federal do Ceara, Fortaleza, Ceará CEP 60 451 970, Brazil
    Naunyn Schmiedebergs Arch Pharmacol 385:455-63. 2012
  5. ncbi request reprint Latex proteins from the plant Calotropis procera are partially digested upon in vitro enzymatic action and are not immunologically detected in fecal material
    Marcio V Ramos
    Departamento de Bioquímica e Biologia Molecular da Universidade Federal do Ceará, Campus do Pici, Cx Postal 6033, Fortaleza Ceara, CEP 60451 970, Brazil
    Fitoterapia 77:251-6. 2006
  6. doi request reprint Involvement of NO in the inhibitory effect of Calotropis procera latex protein fractions on leukocyte rolling, adhesion and infiltration in rat peritonitis model
    Marcio V Ramos
    Departamento de Bioquímica de Biologia Molecular, Universidade Federal do Ceara, Campus do Pici, Cx Postal 6033, Fortaleza Ce Brasil, CEP 60451 970, Brazil
    J Ethnopharmacol 125:387-92. 2009
  7. ncbi request reprint Potential of laticifer fluids for inhibiting Aedes aegypti larval development: evidence for the involvement of proteolytic activity
    Marcio V Ramos
    Departamento de Biologia, Universidade Federal do Ceara, Fortaleza, CE, Brasil, 60451 970
    Mem Inst Oswaldo Cruz 104:805-12. 2009
  8. ncbi request reprint Immunological and allergenic responses induced by latex fractions of Calotropis procera (Ait.) R.Br
    M V Ramos
    Departamento de Bioquimica e Biologia Molecular, Campus do Pici, Universidade Federal do Ceara, Fortaleza, Ceara, CEP, Brazil
    J Ethnopharmacol 111:115-22. 2007
  9. doi request reprint Proteins from latex of Calotropis procera prevent septic shock due to lethal infection by Salmonella enterica serovar Typhimurium
    José V Lima-Filho
    Departamento de Biologia, Universidade Federal Rural de Pernambuco, Campus Dois Irmãos, Recife, PE, Brazil
    J Ethnopharmacol 129:327-34. 2010
  10. doi request reprint Thrombin and plasmin-like activities in the latices of Cryptostegia grandiflora and Plumeria rubra
    Carolina A Viana
    Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza, CE, Brazil
    Blood Coagul Fibrinolysis 24:386-92. 2013

Collaborators

  • Ilka M Vasconcelos
  • Ronaldo A Ribeiro
  • L V Costa-Lotufo
  • B S Cavada
  • Silvania M M Vasconcelos
  • Mariana L Vale
  • Fernando Q Cunha
  • C Pessoa
  • J J Calvete
  • Carlos E Salas
  • Cleverson D T Freitas
  • José V Lima-Filho
  • Nylane M N Alencar
  • Renato A Moreira
  • Carolina A Viana
  • Ayrles F B Silva
  • N M N Alencar
  • Cleverson D T de Freitas
  • Raquel S B Oliveira
  • Jefferson S Oliveira
  • Manoel O Moraes
  • Ana C O Monteiro-Moreira
  • Maria Z R Silva
  • Maria T Ralph
  • Thiago Lustosa Jucá
  • Gerly Anne C Brito
  • Ana Paula F Freitas
  • Diego P Souza
  • Kristiana C Mousinho
  • Ana Paula N N Alves
  • Daniel P Bezerra
  • N M R Macedo
  • Nívea M R Macedo
  • Paula Matias Soares
  • Ana Cristina O Monteiro-Moreira
  • Nylane M de Alencar
  • Frederico B M B Moreno
  • Mayara P V Matos
  • Wallace T da Cruz
  • Frederico B B Moreno
  • Bruno A M Rocha
  • Luciana M R Alencar
  • Hermogenes D Oliveira
  • Jeanlex S Sousa
  • Daiane L Silva
  • Frederico Bruno-Moreno
  • Nadia A P Nogueira
  • José Delano Barreto Marinho-Filho
  • Ana Cristina de Oliveira Monteiro-Moreira
  • Renato Azevedo Moreira
  • Frederico Bruno Mendes Batista Moreno
  • Cristina P S Carvalho
  • Mayara Patrícia Viana de Matos
  • Beatriz C Nishi
  • Nylane Maria N de Alencar
  • Roberto César P Lima-Júnior
  • Flavio S Bitencourt
  • Rachel S P Pinheiro
  • Ingrid S T Figueiredo
  • Lyara B N Freitas
  • Danielle A Pereira
  • Fábio C Nogueira
  • Adriana A Carvalho
  • Mayara Patrícia V de Matos
  • Hemerson Iury F Magalhães
  • Cecília de C Oliveira
  • Fredy D A Silva
  • José Roberto de O Ferreira
  • José Delano B Marinho-Filho
  • L R Bomfim
  • Ingrid Samantha T Figueiredo
  • M P V Matos
  • R S B Oliveira
  • Nicodemos T Filho
  • Joyce M Patriota
  • I J M Cavalcante
  • J G Figueiredo
  • J V S Nunes
  • L R Bonfim
  • M O Moraes
  • Manoel C A Patrocínio
  • Diego V Wilke
  • Silvane R Lima
  • Samara G Matos
  • Krishnamurti M Carvalho
  • David N Criddle
  • Marcelo M Andrade
  • Bruno A Cardi
  • Liezelotte R Bomfim
  • Ana Maria S Assreuy

Detail Information

Publications32

  1. doi request reprint Peptidases and peptidase inhibitors in gut of caterpillars and in the latex of their host plants
    Marcio V Ramos
    Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Campus do Pici, Cx Postal 6033, Fortaleza, CE, CEP 60451 970, Brazil
    Planta 241:167-78. 2015
    ....
  2. pmc Insights on the phytochemical profile (cyclopeptides) and biological activities of Calotropis procera latex organic fractions
    Thiago Lustosa Jucá
    Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Av Humberto Monte, Campus do Pici, 60451 970 Fortaleza, CE, Brazil
    ScientificWorldJournal 2013:615454. 2013
    ..Cyclopeptide detection is especially interesting because previous attempts to investigate these low-molecular cyclic amino acid sequences in C. procera have failed. ..
  3. doi request reprint New insights into the complex mixture of latex cysteine peptidases in Calotropis procera
    M V Ramos
    Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Fortaleza, CE, Brazil
    Int J Biol Macromol 58:211-9. 2013
    ..The purified enzymes exhibited plasma-clotting activity mediated by a thrombin-like mechanism. The set of results suggest the three isolated polypeptides correspond to different post-translationally processed forms of the same protein...
  4. doi request reprint Proteins derived from latex of C. procera maintain coagulation homeostasis in septic mice and exhibit thrombin- and plasmin-like activities
    Marcio V Ramos
    Departamento de Bioquimica e Biologia Molecular, Campus do Pici, bloco 907, Universidade Federal do Ceara, Fortaleza, Ceará CEP 60 451 970, Brazil
    Naunyn Schmiedebergs Arch Pharmacol 385:455-63. 2012
    ..procera exhibit thrombin- and plasmin-like activities and suggests that these proteins have therapeutic potential in various conditions associated with coagulation abnormalities...
  5. ncbi request reprint Latex proteins from the plant Calotropis procera are partially digested upon in vitro enzymatic action and are not immunologically detected in fecal material
    Marcio V Ramos
    Departamento de Bioquímica e Biologia Molecular da Universidade Federal do Ceará, Campus do Pici, Cx Postal 6033, Fortaleza Ceara, CEP 60451 970, Brazil
    Fitoterapia 77:251-6. 2006
    ..Taken together these results suggest that harmful and toxic effects on animals of the latex from C. procera are present in its rubber and low molecular weight fractions rather than its protein content...
  6. doi request reprint Involvement of NO in the inhibitory effect of Calotropis procera latex protein fractions on leukocyte rolling, adhesion and infiltration in rat peritonitis model
    Marcio V Ramos
    Departamento de Bioquímica de Biologia Molecular, Universidade Federal do Ceara, Campus do Pici, Cx Postal 6033, Fortaleza Ce Brasil, CEP 60451 970, Brazil
    J Ethnopharmacol 125:387-92. 2009
    ..The present study was carried out to evaluate the effect of LP sub-fractions on neutrophil functions and nociception in rodent models and to elucidate the mediatory role of nitric oxide (NO)...
  7. ncbi request reprint Potential of laticifer fluids for inhibiting Aedes aegypti larval development: evidence for the involvement of proteolytic activity
    Marcio V Ramos
    Departamento de Biologia, Universidade Federal do Ceara, Fortaleza, CE, Brasil, 60451 970
    Mem Inst Oswaldo Cruz 104:805-12. 2009
    ..aegypti and thus could prove useful for combating mosquito proliferation. This is the first report to present evidence for the use of proteolytic enzymes as chemical agents to destroy Ae. aegypti larvae...
  8. ncbi request reprint Immunological and allergenic responses induced by latex fractions of Calotropis procera (Ait.) R.Br
    M V Ramos
    Departamento de Bioquimica e Biologia Molecular, Campus do Pici, Universidade Federal do Ceara, Fortaleza, Ceara, CEP, Brazil
    J Ethnopharmacol 111:115-22. 2007
    ..It should be relevant to determine whether NDL could induce such activities when assayed by oral route since it was ineffective to induce allergy by this way...
  9. doi request reprint Proteins from latex of Calotropis procera prevent septic shock due to lethal infection by Salmonella enterica serovar Typhimurium
    José V Lima-Filho
    Departamento de Biologia, Universidade Federal Rural de Pernambuco, Campus Dois Irmãos, Recife, PE, Brazil
    J Ethnopharmacol 129:327-34. 2010
    ..In this work the anti-inflammatory protein fraction was evaluated in a true inflammatory process by inducing a lethal experimental infection in the murine model caused by Salmonella enterica Subsp. enterica serovar Typhimurium...
  10. doi request reprint Thrombin and plasmin-like activities in the latices of Cryptostegia grandiflora and Plumeria rubra
    Carolina A Viana
    Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza, CE, Brazil
    Blood Coagul Fibrinolysis 24:386-92. 2013
    ..These results suggested that fibrinogenolytic and procoagulant activities of C. grandiflora were performed by cysteine proteases and confirm the activity of latex cysteine proteases as thrombin and plasmin-like proteins...
  11. doi request reprint Inflammation induced by phytomodulatory proteins from the latex of Calotropis procera (Asclepiadaceae) protects against Salmonella infection in a murine model of typhoid fever
    Raquel S B Oliveira
    Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Campus do Pici, CX, Postal 6033, Fortaleza, Ceara, CEP 60451 970, Brazil
    Inflamm Res 61:689-98. 2012
    ..Typhimurium was investigated...
  12. doi request reprint Laticifer proteins play a defensive role against hemibiotrophic and necrotrophic phytopathogens
    Diego P Souza
    Departamento de Bioquímica e Biologia, Molecular da Universidade Federal do Ceará, Campus do Pici, Cx Postal 6033, Fortaleza, Ceara, CEP 60451 970, Brazil
    Planta 234:183-93. 2011
    ..The results support that laticifer proteins are part of plant defense repertoire against phytopathogenic fungi...
  13. doi request reprint Antitumor effect of laticifer proteins of Himatanthus drasticus (Mart.) Plumel - Apocynaceae
    Kristiana C Mousinho
    Departamento de Fisiologia e Farmacologia, Faculdade de Medicina, Universidade Federal do Ceara, Fortaleza, Ceara, Brazil
    J Ethnopharmacol 137:421-6. 2011
    ..Its bark and latex have been used by the public for cancer treatment, among other medicinal uses. However, there is almost no scientific research report on its medicinal properties...
  14. doi request reprint Protein fraction of Calotropis procera latex protects against 5-fluorouracil-induced oral mucositis associated with downregulation of pivotal pro-inflammatory mediators
    Ana Paula F Freitas
    Department of Clinical Medicine, Faculty of Medicine, Federal University of Ceara, 60430 140, Fortaleza, CE, Brazil
    Naunyn Schmiedebergs Arch Pharmacol 385:981-90. 2012
    ..These findings demonstrate an anti-inflammatory effect of LP on 5-FU-induced oral mucositis. The protective mechanism appears to involve inhibition of the expression of iNOS, COX-2, TNF-α, and IL-1β...
  15. ncbi request reprint Anti-inflammatory effect of the latex from Calotropis procera in three different experimental models: peritonitis, paw edema and hemorrhagic cystitis
    N M N Alencar
    Departamento de Farmacologia e Fisiologia, Universidade Federal do Ceara, Campus do Pici, Fortaleza Ceara, Brasil
    Planta Med 70:1144-9. 2004
    ..The results also suggest that the active molecules are of a proteinaceous nature despite the presence of numerous secondary metabolites naturally occurring in the C. procera latex...
  16. doi request reprint Proteomic analysis and purification of an unusual germin-like protein with proteolytic activity in the latex of Thevetia peruviana
    Cleverson D T de Freitas
    Departamento de Bioquímica e Biologia Molecular da Universidade Federal do Ceará, Campus do Pici, Cx Postal 6033, Fortaleza, CE, CEP 60451 970, Brazil
    Planta 243:1115-28. 2016
    ..Peruvianin-I represents the first germin-like protein (GLP) with cysteine peptidase activity, an activity unknown in the GLP family so far...
  17. ncbi request reprint Embryotoxic activity and differential binding of plant-derived carbohydrate-recognizing proteins towards the sea urchin embryo cells
    Nívea M R Macedo
    Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Campus do Pici, Cx Postal 6033, Fortaleza Ceara, CEP 60451 970, Brazil
    Int J Biol Macromol 36:90-7. 2005
    ..This property could be useful in analyzing other cell types...
  18. doi request reprint In vivo growth inhibition of sarcoma 180 by latex proteins from Calotropis procera
    Jefferson S Oliveira
    Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Campus do Pici, Caixa Postal 6033, 60 451 970, Fortaleza, Ceara, Brazil
    Naunyn Schmiedebergs Arch Pharmacol 382:139-49. 2010
    ..This study confirms the pharmacological potential of proteins from the latex of C. procera to control sarcoma cell proliferation...
  19. doi request reprint First insights into the diversity and functional properties of chitinases of the latex of Calotropis procera
    Cleverson D T Freitas
    Departamento de Bioquímica e Biologia Molecular da Universidade Federal do Ceará, Campus do Pici, Cx Postal 6033, Fortaleza, CE CEP 60451 970, Brazil Electronic address
    Plant Physiol Biochem 108:361-371. 2016
    ..It is concluded that closely related chitinases are present in the latex of C. procera, and the first experimental evidence suggests these proteins are involved more efficiently in defence strategies against insects rather than fungi...
  20. ncbi request reprint Isolation and partial characterisation of highly toxic lectins from Abrus pulchellus seeds
    M V Ramos
    Laboratorio de Biología, Departamento de Biologia, Universidade Federal do Ceara, Fortaleza CE, Brazil
    Toxicon 36:477-84. 1998
    ..The antibodies anti-A. pulchellus toxins did not recognize ricin preparation and only partial identity was observed to A. precatorius toxic lectins prepared in a similar way to ricin and A. pulchellus toxins...
  21. doi request reprint Wound healing modulation by a latex protein-containing polyvinyl alcohol biomembrane
    Marcio V Ramos
    Departamento de Bioquímica e Biologia Molecular UFC Campus do Pici 6033, Fortaleza, Ceara, Brazil
    Naunyn Schmiedebergs Arch Pharmacol 389:747-56. 2016
    ..The polyvinyl alcohol membrane was fully absorbed as an inert support while LP was shown to be active. It is therefore concluded that the LP-PVA is a suitable bioresource for biomedical engineering. ..
  22. doi request reprint An anti-inflammatory lectin from Luetzelburgia auriculata seeds inhibits adhesion and rolling of leukocytes and modulates histamine and PGE2 action in acute inflammation models
    N M N Alencar
    Departamento de Fisiologia e Farmacologia, Universidade Federal do Ceara, Fortaleza, CE, Brazil
    Inflamm Res 59:245-54. 2010
    ..To study and characterize the in vivo effect of the lectin from Luetzelburgia auriculata seed on acute inflammation models...
  23. doi request reprint Comparison of immunomodulatory properties of mannose-binding lectins from Canavalia brasiliensis and Cratylia argentea in a mice model of Salmonella infection
    Ayrles F B Silva
    Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Fortaleza, CE, Brazil
    Int Immunopharmacol 31:233-8. 2016
    ..We conclude that ConBr and CFL are prospective phytotherapeutics capable of modulate the cascade of pro-inflammatory plus regulatory cytokines and nitric oxide release derived from systemic infections...
  24. ncbi request reprint Antinociceptive activity of Calotropis procera latex in mice
    Paula Matias Soares
    Laboratorio de Neurofarmacologia, Extensão do Lab de Farmacologia Molecular e Toxinologia, Núcleo de Toxinologia de Venenos Animais, Instituto Superior de Ciências Biomédicas, Universidade Estadual do Ceara, 740 000 Fortaleza, CE, Brazil
    J Ethnopharmacol 99:125-9. 2005
    ..It was concluded that the protein fraction derived from the whole latex of Calotropis procera possesses antinociceptive activity, which is independent of the opioid system...
  25. ncbi request reprint Seed lectin from pisum arvense: isolation, biochemical characterization and amino acid sequence
    Benildo S Cavada
    Departamento de Bioquimica e Biologia Molecular, Campus do Pici, Caixa Postal 6033, Fortaleza Ceará CEP 60451 970, Brazil
    Protein Pept Lett 10:607-17. 2003
    ..It is discussed that P. arvense should be considered as a synonym of P. sativum. Furthermore, like pea lectin, PAL discriminates biantennary fucosylated glycan, determined by surface plasmon resonance...
  26. doi request reprint Crystal structure of an antifungal osmotin-like protein from Calotropis procera and its effects on Fusarium solani spores, as revealed by atomic force microscopy: Insights into the mechanism of action
    Marcio V Ramos
    Departamento de Bioquimica e Biologia Molecular, Centro de Ciencias, Universidade Federal do Ceara, Fortaleza, Ceara, Brazil
    Phytochemistry 119:5-18. 2015
    ..Based on the structural features of CpOsm and the effects that the protein produces on F. solani spores, a possible mechanism of action is suggested and discussed. ..
  27. doi request reprint New constitutive latex osmotin-like proteins lacking antifungal activity
    Cleverson D T Freitas
    Departamento de Bioquímica e Biologia Molecular da Universidade Federal do Ceará, Campus do Pici, Cx Postal 6033, Fortaleza, Ceara, CEP 60451 970, Brazil Electronic address
    Plant Physiol Biochem 96:45-52. 2015
    ..These results suggest that osmotins of different latex sources may be involved in distinct physiological or defensive events. ..
  28. ncbi request reprint The galactose-binding lectin from Vatairea macrocarpa seeds induces in vivo neutrophil migration by indirect mechanism
    Nylane M N Alencar
    Departamentos de Fisiologia e Farmacologia, Universidade Federal do Ceara, 60 451 970 Ceará, Fortaleza, Brazil
    Int J Biochem Cell Biol 35:1674-81. 2003
    ..The blocking actions of dexamethasone and thalidimide suggest that as yet unidentified pro-inflammatory mediators are involved...
  29. ncbi request reprint Characterization of the sugar-binding specificity of the toxic lectins isolated from Abrus pulchellus seeds
    M V Ramos
    Departamentos de Biologia, Universidade Federal do Ceara, Campus do Pici, Cx Postal 6033, Fortaleza Ceara, Brasil, 60 451 970
    Glycoconj J 18:391-400. 2001
    ....
  30. ncbi request reprint Interaction of Diocleinae lectins with glycoproteins based in surface plasmon resonance
    Marcio V Ramos
    Departamento de Biologia, Universidade Federal do Ceara, Fortaleza, CE, 60451 970, Brasil
    Mem Inst Oswaldo Cruz 97:275-9. 2002
    ..This is the first report using surface plasmon resonance technology that evidences differences of Diocleinae lectins in respect their fine glycan-specificity...
  31. ncbi request reprint Expression and purification of the recombinant ConBr (Canavalia brasiliensis lectin) produced in Escherichia coli cells
    Nadia A P Nogueira
    Depto de Análises Clínicas e Toxicológicas, Universidade Federal do Ceara, Ceara, Brasil
    Protein Pept Lett 9:59-66. 2002
    ..By SDS-PAGE and Western blot, rConBr was highly pure with an apparent molecular mass of 37 kDa. N-terminal sequence analysis revealed a single sequence, confirming the identity of the expressed protein as the pre-pro-ConBr...
  32. ncbi request reprint Inhibition of sea urchin fertilization by plant lectins
    N M R Macedo
    Departamento de Bioquimica e Biologia Molecular, Universidade Federal do Ceara, Campus do Pici, Cx Postal 6033 Fortaleza Ceará, CEP 60451 970, Brasil
    Protein Pept Lett 14:886-93. 2007
    ..Pre-incubation of Glucose/mannose binding lectins with eggs did not alter binding of Gal/GalNAc lectins. Lectins with distinct competencies for binding monosaccharide and glycoconjugates were able to inhibit sea urchin fertilization...