Zhigao Bu

Summary

Publications

  1. pmc Recombinant lentogenic Newcastle disease virus expressing Ebola virus GP infects cells independently of exogenous trypsin and uses macropinocytosis as the major pathway for cell entry
    Zhiyuan Wen
    State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, People s Republic of China
    Virol J 10:331. 2013
  2. pmc Rescue of recombinant peste des petits ruminants virus: creation of a GFP-expressing virus and application in rapid virus neutralization test
    Qianqian Hu
    College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, People s Republic of China
    Vet Res 43:48. 2012
  3. pmc Identification of a conserved JEV serocomplex B-cell epitope by screening a phage-display peptide library with a mAb generated against West Nile virus capsid protein
    En Cheng Sun
    The Key Laboratory of Veterinary Public Health, Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, PR China
    Virol J 8:100. 2011
  4. pmc Identification and characterization of a virus-specific continuous B-cell epitope on the PrM/M protein of Japanese Encephalitis Virus: potential application in the detection of antibodies to distinguish Japanese Encephalitis Virus infection from West Nile
    Rong Hong Hua
    State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, PR China
    Virol J 7:249. 2010
  5. pmc Identification of two linear B-cell epitopes from West Nile virus NS1 by screening a phage-displayed random peptide library
    En Cheng Sun
    The Key Laboratory of Veterinary Public Health, Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin 150001, PR China
    BMC Microbiol 11:160. 2011
  6. pmc Newcastle disease virus-vectored rabies vaccine is safe, highly immunogenic, and provides long-lasting protection in dogs and cats
    Jinying Ge
    Harbin Veterinary Research Institute, CAAS, 427 Maduan Street, Harbin 150001, People s Republic of China
    J Virol 85:8241-52. 2011
  7. doi request reprint A goat poxvirus-vectored peste-des-petits-ruminants vaccine induces long-lasting neutralization antibody to high levels in goats and sheep
    Weiye Chen
    Key Laboratory of Veterinary Public Health of Ministry of Agriculture and State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, PR China
    Vaccine 28:4742-50. 2010
  8. pmc Generation of a recombinant rabies Flury LEP virus carrying an additional G gene creates an improved seed virus for inactivated vaccine production
    Lihong Tao
    State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, PR China
    Virol J 8:454. 2011
  9. pmc Molecular basis of neurovirulence of flury rabies virus vaccine strains: importance of the polymerase and the glycoprotein R333Q mutation
    Lihong Tao
    Veterinary Public Health Laboratory of Ministry of Agriculture, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, People s Republic of China
    J Virol 84:8926-36. 2010
  10. doi request reprint H5N1 influenza marker vaccine for serological differentiation between vaccinated and infected chickens
    Chengjun Li
    Harbin Veterinary Research Institute, CAAS, 427 Maduan Street, Harbin, Heilongjiang 150001, People s Republic of China
    Biochem Biophys Res Commun 372:293-7. 2008

Collaborators

Detail Information

Publications63

  1. pmc Recombinant lentogenic Newcastle disease virus expressing Ebola virus GP infects cells independently of exogenous trypsin and uses macropinocytosis as the major pathway for cell entry
    Zhiyuan Wen
    State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, People s Republic of China
    Virol J 10:331. 2013
    ....
  2. pmc Rescue of recombinant peste des petits ruminants virus: creation of a GFP-expressing virus and application in rapid virus neutralization test
    Qianqian Hu
    College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, 210095, People s Republic of China
    Vet Res 43:48. 2012
    ..We found that our recombinant virus allowed more rapid and higher throughput assessment of PPRV neutralization antibody titer via the virus neutralization test (VNT) compared with the traditional method...
  3. pmc Identification of a conserved JEV serocomplex B-cell epitope by screening a phage-display peptide library with a mAb generated against West Nile virus capsid protein
    En Cheng Sun
    The Key Laboratory of Veterinary Public Health, Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, PR China
    Virol J 8:100. 2011
    ..The antigenic sites on C protein that are targeted by humoral immune responses have not been studied thoroughly, and well-defined B-cell epitopes on the WNV C protein have not been reported...
  4. pmc Identification and characterization of a virus-specific continuous B-cell epitope on the PrM/M protein of Japanese Encephalitis Virus: potential application in the detection of antibodies to distinguish Japanese Encephalitis Virus infection from West Nile
    Rong Hong Hua
    State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, PR China
    Virol J 7:249. 2010
    ..Virus specific epitopes could benefit for developing JEV specific antibodies detection methods. To identify the JEV specific epitopes, we fully mapped and characterized the continuous B-cell epitope of the PrM/M protein of JEV...
  5. pmc Identification of two linear B-cell epitopes from West Nile virus NS1 by screening a phage-displayed random peptide library
    En Cheng Sun
    The Key Laboratory of Veterinary Public Health, Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin 150001, PR China
    BMC Microbiol 11:160. 2011
    ..Although previous work has demonstrated the vital role of WNV NS1-specific antibody responses, the specific epitopes in the NS1 have not been identified...
  6. pmc Newcastle disease virus-vectored rabies vaccine is safe, highly immunogenic, and provides long-lasting protection in dogs and cats
    Jinying Ge
    Harbin Veterinary Research Institute, CAAS, 427 Maduan Street, Harbin 150001, People s Republic of China
    J Virol 85:8241-52. 2011
    ..This is the first study to demonstrate that immunization with an NDV-vectored vaccine can induce long-lasting, systemic protective immunity against rabies...
  7. doi request reprint A goat poxvirus-vectored peste-des-petits-ruminants vaccine induces long-lasting neutralization antibody to high levels in goats and sheep
    Weiye Chen
    Key Laboratory of Veterinary Public Health of Ministry of Agriculture and State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, PR China
    Vaccine 28:4742-50. 2010
    ..The recombinant CPV vaccine used in our studies could be a practical and useful candidate DIVA vaccine in countries where PPR newly emerges or where stamp-out plans are yet to be implemented...
  8. pmc Generation of a recombinant rabies Flury LEP virus carrying an additional G gene creates an improved seed virus for inactivated vaccine production
    Lihong Tao
    State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, PR China
    Virol J 8:454. 2011
    ..Our results suggest that rLEP-G is an improved seed virus candidate for inactivated rabies virus vaccine manufacture...
  9. pmc Molecular basis of neurovirulence of flury rabies virus vaccine strains: importance of the polymerase and the glycoprotein R333Q mutation
    Lihong Tao
    Veterinary Public Health Laboratory of Ministry of Agriculture, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, People s Republic of China
    J Virol 84:8926-36. 2010
    ..These results indicate that attenuation of the RV Flury strain results from the coevolution of G and L elements and provide important information for the generation of safer and more effective modified live rabies vaccine...
  10. doi request reprint H5N1 influenza marker vaccine for serological differentiation between vaccinated and infected chickens
    Chengjun Li
    Harbin Veterinary Research Institute, CAAS, 427 Maduan Street, Harbin, Heilongjiang 150001, People s Republic of China
    Biochem Biophys Res Commun 372:293-7. 2008
    ..H5N1/PR8-5B19 is therefore an attractive marker vaccine candidate, eliciting a strong, protective antibody response and enabling serological discrimination between vaccinated and wild-type virus-infected chickens...
  11. doi request reprint Recombinant canine distemper virus serves as bivalent live vaccine against rabies and canine distemper
    Xijun Wang
    Key Laboratory of Veterinary Public Health of Ministry of Agriculture, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
    Vaccine 30:5067-72. 2012
    ..This is the first study demonstrating that recombinant CDV has the potential to serve as bivalent live vaccine against rabies and canine distemper in animals...
  12. doi request reprint Two amino acid residues in the matrix protein M1 contribute to the virulence difference of H5N1 avian influenza viruses in mice
    Shufang Fan
    Animal Influenza Laboratory of the Ministry of Agriculture and National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, Harbin, PR China
    Virology 384:28-32. 2009
    ..We thus firstly prove the M1 protein contributes to the virulence of H5N1 viruses in mice, and the amino acid residues shown to attenuate the virulence could be targeted in influenza virus candidates for live vaccine development...
  13. doi request reprint H7N9 influenza viruses are transmissible in ferrets by respiratory droplet
    Qianyi Zhang
    State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, People s Republic of China
    Science 341:410-4. 2013
    ..Most importantly, one virus isolated from humans was highly transmissible in ferrets by respiratory droplet. Our findings indicate nothing to reduce the concern that these viruses can transmit between humans. ..
  14. doi request reprint H5N1 hybrid viruses bearing 2009/H1N1 virus genes transmit in guinea pigs by respiratory droplet
    Ying Zhang
    State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People s Republic of China
    Science 340:1459-63. 2013
    ..Hence, avian H5N1 subtype viruses do have the potential to acquire mammalian transmissibility by reassortment in current agricultural scenarios. ..
  15. pmc Immunogenicity and protective efficacy of a live attenuated H5N1 vaccine in nonhuman primates
    Shufang Fan
    Animal Influenza Laboratory of the Ministry of Agriculture and National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, PR China
    PLoS Pathog 5:e1000409. 2009
    ....
  16. pmc Continued evolution of H5N1 influenza viruses in wild birds, domestic poultry, and humans in China from 2004 to 2009
    Yanbing Li
    Animal Influenza Laboratory of the Ministry of Agriculture and National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, 427 Maduan Street, Harbin 150001, People s Republic of China
    J Virol 84:8389-97. 2010
    ..Our results demonstrate that the H5N1 AIVs circulating in nature have complex biological characteristics and pose a continued challenge for disease control and pandemic preparedness...
  17. doi request reprint Establishment of a stable CHO cell line with high level expression of recombinant porcine IFN-β
    Weiye Chen
    National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences CAAS, 427 Maduan Street, Harbin 150001, People s Republic of China
    Cytokine 54:324-9. 2011
    ..This is the first report of establishing a mammalian cell line with stable expression of porcine IFN-β...
  18. pmc Key molecular factors in hemagglutinin and PB2 contribute to efficient transmission of the 2009 H1N1 pandemic influenza virus
    Ying Zhang
    State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People s Republic of China
    J Virol 86:9666-74. 2012
    ....
  19. doi request reprint Newcastle disease virus-vectored Nipah encephalitis vaccines induce B and T cell responses in mice and long-lasting neutralizing antibodies in pigs
    Dongni Kong
    State Key Laboratory of Veterinary Biotechnology and Animal Influenza Laboratory of the Ministry of Agriculture, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, People s Republic of China
    Virology 432:327-35. 2012
    ..Recombinant rLa-NiVG/F thus appear to be promising NiV vaccine candidates for pigs and potentially humans...
  20. pmc A naturally occurring deletion in its NS gene contributes to the attenuation of an H5N1 swine influenza virus in chickens
    Qiyun Zhu
    Harbin Veterinary Research Institute, 427 Maduan Street, Harbin 150001, People s Republic of China
    J Virol 82:220-8. 2008
    ....
  21. doi request reprint VP5-deficient mutant virus induced protection against challenge with very virulent infectious bursal disease virus of chickens
    Liting Qin
    Division of Avian Infectious Diseases, National State Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin 150001, PR China
    Vaccine 28:3735-40. 2010
    ..All data indicated that VP5 played an important role in viral replication and pathogenesis both in vitro and in vivo. The VP5-deficient mutant virus could be a good candidate as a marked vaccine...
  22. ncbi request reprint Enhanced protective efficacy of H5 subtype avian influenza DNA vaccine with codon optimized HA gene in a pCAGGS plasmid vector
    Yongping Jiang
    Animal Influenza Laboratory of the Ministry of Agriculture, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, People s Republic of China
    Antiviral Res 75:234-41. 2007
    ....
  23. doi request reprint Expression of biologically active recombinant equine interferon-gamma in Escherichia coli
    Yu Bai
    Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, PR China
    Comp Immunol Microbiol Infect Dis 33:333-42. 2010
    ..Pure forms and sufficient quantities of biologically active IFN-gamma could facilitate the study of its activities in modulating immune responses both in vivo and in vitro...
  24. pmc A duck enteritis virus-vectored bivalent live vaccine provides fast and complete protection against H5N1 avian influenza virus infection in ducks
    Jinxiong Liu
    State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, 427 Maduan Street, Harbin 150001, People s Republic of China
    J Virol 85:10989-98. 2011
    ..These results demonstrate, for the first time, that recombinant DEV is suitable for use as a bivalent live attenuated vaccine, providing rapid protection against both DEV and H5N1 virus infection in ducks...
  25. pmc Immunization by influenza virus-like particles protects aged mice against lethal influenza virus challenge
    Zhiyuan Wen
    National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, People s Republic of China
    Antiviral Res 84:215-24. 2009
    ....
  26. pmc Characterization of immune responses induced by immunization with the HA DNA vaccines of two antigenically distinctive H5N1 HPAIV isolates
    Yulong Gao
    National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People s Republic of China
    PLoS ONE 7:e41332. 2012
    ..These results indicate the existence of original antigenic sin (OAS) between concurrently circulating H5N1 HPAIV strains, which may need to be taken into consideration in vaccine development against the potential H5N1 HPAIV pandemic...
  27. ncbi request reprint Protective efficacy of H7 subtype avian influenza DNA vaccine
    Yongping Jiang
    Animal Influenza Laboratory of the Ministry of Agriculture and State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, People s Republic of China
    Avian Dis 54:290-3. 2010
    ..These results provide a strong argument for the continued evaluation of this vaccine in field trials...
  28. pmc A single-amino-acid substitution in the NS1 protein changes the pathogenicity of H5N1 avian influenza viruses in mice
    Peirong Jiao
    Harbin Veterinary Research Institute, CAAS, 427 Maduan Street, Harbin 150001, People s Republic of China
    J Virol 82:1146-54. 2008
    ..Our results indicate that the NS1 protein is critical for the pathogenicity of H5N1 influenza viruses in mammalian hosts and that the amino acid S42 of NS1 plays a key role in undermining the antiviral immune response of the host cell...
  29. pmc Dogs are highly susceptible to H5N1 avian influenza virus
    Ying Chen
    Animal Influenza Laboratory of the Ministry of Agriculture and National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, People s Republic of China
    Virology 405:15-9. 2010
    ..Our results demonstrate that dogs are highly susceptible to H5N1 AIV and may serve as an intermediate host to transfer this virus to humans...
  30. pmc Identification of amino acids in HA and PB2 critical for the transmission of H5N1 avian influenza viruses in a mammalian host
    Yuwei Gao
    Animal Influenza Laboratory of the Ministry of Agriculture and National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People s Republic of China
    PLoS Pathog 5:e1000709. 2009
    ..These amino acids changes in PB2 and HA could serve as important molecular markers for assessing the pandemic potential of H5N1 field isolates...
  31. doi request reprint Recombinant duck enteritis virus works as a single-dose vaccine in broilers providing rapid protection against H5N1 influenza infection
    Jinxiong Liu
    Animal Influenza Laboratory, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, 427 Maduan Street, Harbin 150001, PR China
    Antiviral Res 97:329-33. 2013
    ..We found this new rDEV-re6 virus to be safe in chickens and to induce rapid and solid protection after a single dose. This virus may therefore serve as an ideal single-dose vaccine for broilers...
  32. pmc Identification of CD8+ cytotoxic T lymphocyte epitopes from porcine reproductive and respiratory syndrome virus matrix protein in BALB/c mice
    Weijun Zhang
    The Key Laboratory of Veterinary Public Health, Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, PR China
    Virol J 8:263. 2011
    ....
  33. ncbi request reprint [Cloning of infectious bursal disease virus (Gt strain) lack of VP5 gene]
    Liting Qin
    Division of Avian Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
    Wei Sheng Wu Xue Bao 48:1666-70. 2008
    ..The genome of IBDV consists of two segments of double-strand RNA, which encode four structural protein VP1-VP4 and one non-structural protein VP5...
  34. pmc The PA protein directly contributes to the virulence of H5N1 avian influenza viruses in domestic ducks
    Jiasheng Song
    Harbin Veterinary Research Institute, CAAS, Harbin 150001, People s Republic of China
    J Virol 85:2180-8. 2011
    ..Our results provide strong evidence that the polymerase PA subunit is a virulence factor for H5N1 AIVs in ducks...
  35. doi request reprint DNA-chitosan nanoparticles improve DNA vaccine-elicited immunity against Newcastle disease virus through shuttling chicken interleukin-2 gene
    Wenlong Zhang
    The Key Laboratory of Veterinary Public Health, Ministry of Agriculture, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
    J Microencapsul 27:693-702. 2010
    ..05). The results demonstrate that pCAGG-ChIL2 CNPs improve DNA vaccine-elicited immunity against NDV challenge...
  36. ncbi request reprint Efficacy of DNA immunization with F and G protein genes of Nipah virus
    Xijun Wang
    National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Science, Harbin 150001, P R China
    Ann N Y Acad Sci 1081:243-5. 2006
    ....
  37. pmc Comprehensive mapping of common immunodominant epitopes in the West Nile virus nonstructural protein 1 recognized by avian antibody responses
    Encheng Sun
    The Key Laboratory of Veterinary Public Health, Harbin Veterinary Research Institute, Ministry of Agriculture, Chinese Academy of Agricultural Sciences, Harbin, People s Republic of China
    PLoS ONE 7:e31434. 2012
    ..The knowledge and reagents generated in this study have potential applications in differential diagnostic approaches and subunit vaccines development for WNV and other viruses of the JEV serocomplex...
  38. doi request reprint Exchange of the VP5 of infectious bursal disease virus in a serotype I strain with that of a serotype II strain reduced the viral replication and cytotoxicity
    Liting Qin
    Division of Avian Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150001, P R China
    J Microbiol 47:344-50. 2009
    ..Therefore, the VP5 of serotype II may be one of the factors responsible for the distinct pathogenic features of two serotypes...
  39. ncbi request reprint [Evaluation of Newcastle disease virus with derivated hemagglutinin-neuraminidase gene of mesogenic strain]
    Yong Wang
    National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
    Wei Sheng Wu Xue Bao 48:638-43. 2008
    ..To understand the exact contribution of the HN gene to NDV pathogenecity, a reverse genetics system was developed using the lentogenic NDV LaSota strain ...
  40. pmc Deep-sequencing analysis of the mouse transcriptome response to infection with Brucella melitensis strains of differing virulence
    Fangkun Wang
    State Key Laboratory of Veterinary Biotechnology and Zoonosis Laboratory of the Ministry of Agriculture, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, People s Republic of China
    PLoS ONE 6:e28485. 2011
    ..Our data provide new insights into the molecular attenuation mechanism of strain M5-90 and will facilitate the generation of new attenuated vaccine strains with enhanced efficacy...
  41. ncbi request reprint Generating vesicular stomatitis virus pseudotype bearing the severe acute respiratory syndrome coronavirus spike envelope glycoprotein for rapid and safe neutralization test or cell-entry assay
    Jinying Ge
    National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, P R China
    Ann N Y Acad Sci 1081:246-8. 2006
    ....
  42. pmc Newcastle disease virus-based live attenuated vaccine completely protects chickens and mice from lethal challenge of homologous and heterologous H5N1 avian influenza viruses
    Jinying Ge
    National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, 427 Maduan Street, Harbin 150001, People s Republic of China
    J Virol 81:150-8. 2007
    ..The recombinant NDV vaccine may also have potential use in high-risk human individuals to control the pandemic spread of lethal avian influenza...
  43. pmc The NS1 gene contributes to the virulence of H5N1 avian influenza viruses
    Zejun Li
    Harbin Veterinary Research Institute, CAAS, 427 Maduan Street, Harbin 150001, People s Republic of China
    J Virol 80:11115-23. 2006
    ....
  44. pmc Comparison of genomes of Brucella melitensis M28 and the B. melitensis M5-90 derivative vaccine strain highlights the translation elongation factor Tu gene tuf2 as an attenuation-related gene
    Fangkun Wang
    State Key Laboratory of Veterinary Biotechnology and Zoonosis Laboratory of the Ministry of Agriculture, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, Heilongjiang, China
    Infect Immun 81:2812-8. 2013
    ..We report that the tuf2 gene plays an important role in the attenuation of M5-90 virulence. ..
  45. ncbi request reprint [Insertion of glycoprotein gene between P and M gene influences the pathogenicity of the rabies virus Flury LEP]
    Hongyue Zhai
    Veterinary Public Health Laboratory of Ministry of Agriculture and State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China
    Wei Sheng Wu Xue Bao 51:1098-105. 2011
    ..To study the biological characteristics and pathogenicity of a recombinant rabies virus Flury LEP (low egg passage) that has two glycoprotein genes (G gene)...
  46. doi request reprint Genetically modified rabies virus ERA strain is safe and induces long-lasting protective immune response in dogs after oral vaccination
    Lei Shuai
    Key Laboratory of Veterinary Public Health of Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
    Antiviral Res 121:9-15. 2015
    ..These results demonstrated that the genetically modified ERA vaccine strain has the potential to serve as a safe and efficient oral live vaccine against rabies in dogs. ..
  47. doi request reprint Protective efficacy of an H1N1 cold-adapted live vaccine against the 2009 pandemic H1N1, seasonal H1N1, and H5N1 influenza viruses in mice
    Jianzhong Shi
    Animal Influenza Laboratory, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, People s Republic of China
    Antiviral Res 93:346-53. 2012
    ..The induction of cross-reactive virus-specific T cell responses may be an effective approach to develop universal influenza vaccines...
  48. ncbi request reprint [Impact of modification of cleavage site of fusion protein and foreign gene insertion on the virulence of Newcastle Disease Virus LaSota vaccine strain]
    Yong Wang
    National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
    Wei Sheng Wu Xue Bao 48:362-8. 2008
    ..These results also indicate that cleavability of the F0 protein is an important determinant for virulence of NDV. NDV can be manipulated in the future for use as a vaccine vector...
  49. ncbi request reprint [Expression of porcine interferon-gamma and its safe antiviral assay]
    Fan He
    State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
    Sheng Wu Gong Cheng Xue Bao 26:439-47. 2010
    ....
  50. doi request reprint Novel in-ovo chimeric recombinant Newcastle disease vaccine protects against both Newcastle disease and infectious bursal disease
    Jinying Ge
    Key Laboratory of Veterinary Public Health of Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China
    Vaccine 32:1514-21. 2014
    ..In-ovo rLaC30L-VP2 vaccination also provided resistance against vvIBDV challenge in a significant amount of animals. These results suggest that rLaC30L-VP2 is a safe and efficient bivalent live in-ovo vaccine against NDV and vvIBDV. ..
  51. ncbi request reprint [Recombinant goat pox virus expressing PPRV H protein]
    Weiye Chen
    State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute of Chinese Academy of Agricultural Sciences, Harbin 150001, China
    Sheng Wu Gong Cheng Xue Bao 25:496-502. 2009
    ..This study established a foundation for the industrialization of the PPRV recombinant GPV vaccine...
  52. pmc Genetics, receptor binding property, and transmissibility in mammals of naturally isolated H9N2 Avian Influenza viruses
    Xuyong Li
    State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin, People s Republic of China State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, People s Republic of China
    PLoS Pathog 10:e1004508. 2014
    ....
  53. doi request reprint Recombinant Newcastle disease viral vector expressing hemagglutinin or fusion of canine distemper virus is safe and immunogenic in minks
    Jinying Ge
    Key Laboratory of Veterinary Public Health of Ministry of Agriculture, State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, China Electronic address
    Vaccine 33:2457-62. 2015
    ..Results suggest that recombinant NDV expressing CDV H is safe and efficient candidate vaccine against CDV in mink, and maybe other host species. ..
  54. ncbi request reprint An improved method for infectious bursal disease virus rescue using RNA polymerase II system
    Xiaole Qi
    Division of Avian Infectious Diseases, National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, The Chinese Academy of Agricultural Sciences, Harbin 150001, PR China
    J Virol Methods 142:81-8. 2007
    ..The system not only provides the basis of the gene function research of IBDV, but is also useful for reverse genetics research of other birnaviridae viruses...
  55. doi request reprint Development and application of avian influenza vaccines in China
    Hualan Chen
    Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin, 150001, People s Republic of China
    Curr Top Microbiol Immunol 333:153-62. 2009
    ..Over 30 billion doses of these vaccines have been used in China and other countries, including Vietnam, Mongolia, and Egypt, and have played an important role in H5N1 avian influenza control in these countries...
  56. pmc Prevalence, genetics, and transmissibility in ferrets of Eurasian avian-like H1N1 swine influenza viruses
    Huanliang Yang
    State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, People s Republic of China
    Proc Natl Acad Sci U S A 113:392-7. 2016
    ..Our study shows the potential of EAH1N1 SIVs to transmit efficiently in humans and suggests that immediate action is needed to prevent the efficient transmission of EAH1N1 SIVs to humans. ..
  57. pmc Protective efficacy of a recombinant Newcastle disease virus expressing glycoprotein of vesicular stomatitis virus in mice
    Minmin Zhang
    State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin, 150001, People s Republic of China
    Virol J 13:31. 2016
    ..In this study, a recombinant Newcastle disease virus (NDV) expressing the glycoprotein (G) of VSV (rL-VSV-G) was constructed and its pathogenicity and immune protective efficacy in mouse were evaluated...
  58. pmc Complete genome sequences of Brucella melitensis strains M28 and M5-90, with different virulence backgrounds
    Fangkun Wang
    Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Harbin 150001, People s Republic of China
    J Bacteriol 193:2904-5. 2011
    ..Here we report the complete genome sequences of B. melitensis M28 and M5-90, strains with different virulence backgrounds, which will serve as a valuable reference for future studies...
  59. ncbi request reprint [Antiviral activity determination of recombinant equine interferon-gamma and identification inhibited antiviral activity of monoclonal antibodies]
    Yu Bai
    National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, Harbin 150001, China
    Sheng Wu Gong Cheng Xue Bao 24:1258-62. 2008
    ..coli and baculovirus, respectively. The antiviral activities of the recombinant eIFN-gamma were highly efficient and specific, as it was blocked by mAbs against eIFN-gamma...
  60. doi request reprint Two different genotypes of H1N2 swine influenza virus isolated in northern China and their pathogenicity in animals
    Huanliang Yang
    State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, People s Republic of China
    Vet Microbiol 175:224-31. 2015
    ..Based on the results, it is possible that the new genotype of the swine H1N2 virus that emerged in China may become widespread in the swine population and pose a potential threat to public health. ..
  61. ncbi request reprint Protective efficacy in chickens, geese and ducks of an H5N1-inactivated vaccine developed by reverse genetics
    Guobin Tian
    Animal Influenza Laboratory of the Ministry of Agriculture, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150001, PR China
    Virology 341:153-62. 2005
    ..These results provide useful information for the application of vaccines to the control of H5N1 avian influenza in poultry, including chickens and domestic waterfowl...
  62. doi request reprint Avian influenza vaccines against H5N1 'bird flu'
    Chengjun Li
    State Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 427 Maduan Street, Nangang District, Harbin, China 150001
    Trends Biotechnol 32:147-56. 2014
    ..Understanding the properties of the available, novel vaccines will allow for the establishment of rational vaccination protocols, which in turn will help the effective control and prevention of H5N1 AI. ..
  63. ncbi request reprint Enhancement of immunogenicity of an HIV Env DNA vaccine by mutation of the Tyr-based endocytosis motif in the cytoplasmic domain
    Zhigao Bu
    National Key Laboratory of Veterinary Biotechnology, Harbin Veterinary Research Institute, CAAS, 427 Maduan Street, Harbin 150001, P R China
    Virology 328:62-73. 2004
    ..Our results suggest that the YXXPhi motif in the HIV Env cytoplasmic domain may play a role in virus evasion of host immune responses through affecting its immunogenicity...