Patrick Provost

Summary

Country: Canada

Publications

  1. pmc Identification of functional microRNAs released through asymmetrical processing of HIV-1 TAR element
    Dominique L Ouellet
    Centre de Recherche en Rhumatologie et Immunologie, Quebec, QC, G1V 4G2, Canada
    Nucleic Acids Res 36:2353-65. 2008
  2. pmc Modulation of microRNA Activity by Semi-microRNAs
    Isabelle Plante
    CHUQ Research Center CHUL Quebec, QC, Canada
    Front Genet 3:99. 2012
  3. pmc Regulation of host gene expression by HIV-1 TAR microRNAs
    Dominique L Ouellet
    Department of Molecular and Cellular Biology, Beckman Research Institute at City of Hope, 1500 E Duarte Road, Duarte, CA 91010, USA
    Retrovirology 10:86. 2013
  4. pmc HIV-l and the microRNA-guided silencing pathway: an intricate and multifaceted encounter
    Patrick Provost
    Centre de Recherche en Rhumatologie et Immunologie, CHUL Research Center, Quebec, Quebec, Canada
    Virus Res 121:107-15. 2006
  5. pmc Ribonuclease activity and RNA binding of recombinant human Dicer
    Patrick Provost
    Department of Medical Biochemistry and Biophysics, Karolinska Institute, Scheeles vag 2, Stockholm, S 171 77, Sweden
    EMBO J 21:5864-74. 2002
  6. pmc MicroRNAs as a molecular basis for mental retardation, Alzheimer's and prion diseases
    Patrick Provost
    CHUL Research Center CHUQ and Faculty of Medicine, Universite Laval, Quebec, QC, Canada
    Brain Res 1338:58-66. 2010
  7. pmc Interpretation and applicability of microRNA data to the context of Alzheimer's and age-related diseases
    Patrick Provost
    CHUL Research Center CHUQ and Faculty of Medicine, Universite Laval, Quebec, QC, G1K 7P4, Canada
    Aging (Albany NY) 2:166-9. 2010
  8. doi request reprint Coactosin-like protein functions as a stabilizing chaperone for 5-lipoxygenase: role of tryptophan 102
    Julia Esser
    Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry II, Karolinska Institutet, S 171 77 Stockholm, Sweden
    Biochem J 425:265-74. 2009
  9. pmc Human Dicer C-terminus functions as a 5-lipoxygenase binding domain
    Vildan Dincbas-Renqvist
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 171 77, Stockholm, Sweden
    Biochim Biophys Acta 1789:99-108. 2009
  10. pmc Refractoriness of hepatitis C virus internal ribosome entry site to processing by Dicer in vivo
    Dominique L Ouellet
    Centre de Recherche en Rhumatologie et Immunologie, CHUL Research Center CHUQ, 2705 Blvd Laurier, Quebec, QC, G1V 4G2, Canada
    J Negat Results Biomed 8:8. 2009

Collaborators

Detail Information

Publications40

  1. pmc Identification of functional microRNAs released through asymmetrical processing of HIV-1 TAR element
    Dominique L Ouellet
    Centre de Recherche en Rhumatologie et Immunologie, Quebec, QC, G1V 4G2, Canada
    Nucleic Acids Res 36:2353-65. 2008
    ..Our study suggests that the TAR element of HIV-1 transcripts releases functionally competent miRNAs upon asymmetrical processing by Dicer, thereby providing novel insights into viral miRNA biogenesis...
  2. pmc Modulation of microRNA Activity by Semi-microRNAs
    Isabelle Plante
    CHUQ Research Center CHUL Quebec, QC, Canada
    Front Genet 3:99. 2012
    ..Our findings suggest that the 12-nt RNA species, generated along the microRNA pathway, may participate to the control of gene expression by regulating the activity of the related full-length mature microRNA in vivo...
  3. pmc Regulation of host gene expression by HIV-1 TAR microRNAs
    Dominique L Ouellet
    Department of Molecular and Cellular Biology, Beckman Research Institute at City of Hope, 1500 E Duarte Road, Duarte, CA 91010, USA
    Retrovirology 10:86. 2013
    ..The objective of this study was to characterize the post-transcriptional regulation of host messenger RNAs (mRNAs) relevant to HIV-1 pathogenesis by HIV-1 TAR miRNAs...
  4. pmc HIV-l and the microRNA-guided silencing pathway: an intricate and multifaceted encounter
    Patrick Provost
    Centre de Recherche en Rhumatologie et Immunologie, CHUL Research Center, Quebec, Quebec, Canada
    Virus Res 121:107-15. 2006
    ..As we learn more about the mechanism and importance of small RNA-based antiviral systems, a more intricate picture of the interaction between HIV-1 and a proven antiviral defense mechanism in lower eukaryotes is emerging...
  5. pmc Ribonuclease activity and RNA binding of recombinant human Dicer
    Patrick Provost
    Department of Medical Biochemistry and Biophysics, Karolinska Institute, Scheeles vag 2, Stockholm, S 171 77, Sweden
    EMBO J 21:5864-74. 2002
    ..Availability of the recombinant Dicer protein will help improve our understanding of RNA silencing and other Dicer-related processes...
  6. pmc MicroRNAs as a molecular basis for mental retardation, Alzheimer's and prion diseases
    Patrick Provost
    CHUL Research Center CHUQ and Faculty of Medicine, Universite Laval, Quebec, QC, Canada
    Brain Res 1338:58-66. 2010
    ....
  7. pmc Interpretation and applicability of microRNA data to the context of Alzheimer's and age-related diseases
    Patrick Provost
    CHUL Research Center CHUQ and Faculty of Medicine, Universite Laval, Quebec, QC, G1K 7P4, Canada
    Aging (Albany NY) 2:166-9. 2010
    ..However, whether miRNA data can be extrapolated and transposed to the human context of age-related diseases, such as AD, not only requires caution, but also warrants several considerations...
  8. doi request reprint Coactosin-like protein functions as a stabilizing chaperone for 5-lipoxygenase: role of tryptophan 102
    Julia Esser
    Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry II, Karolinska Institutet, S 171 77 Stockholm, Sweden
    Biochem J 425:265-74. 2009
    ..The results of the present study support a role for CLP as a chaperoning scaffold factor, influencing both the stability and the activity of 5-LO...
  9. pmc Human Dicer C-terminus functions as a 5-lipoxygenase binding domain
    Vildan Dincbas-Renqvist
    Department of Medical Biochemistry and Biophysics, Karolinska Institutet, S 171 77, Stockholm, Sweden
    Biochim Biophys Acta 1789:99-108. 2009
    ..Providing a link between miRNA-mediated regulation of gene expression and inflammation, our results suggest that the formation of miRNAs may be regulated by 5LO in leukocytes and cancer cells expressing this lipoxygenase...
  10. pmc Refractoriness of hepatitis C virus internal ribosome entry site to processing by Dicer in vivo
    Dominique L Ouellet
    Centre de Recherche en Rhumatologie et Immunologie, CHUL Research Center CHUQ, 2705 Blvd Laurier, Quebec, QC, G1V 4G2, Canada
    J Negat Results Biomed 8:8. 2009
    ..Important for initiating translation of viral RNAs into proteins, the HCV IRES is composed of RNA structures reminiscent of microRNA precursors that may be targeted by the host RNA silencing machinery...
  11. pmc Existence of a microRNA pathway in anucleate platelets
    Patricia Landry
    Centre de Recherche en Rhumatologie et Immunologie, CHUL Research Center CHUQ, and Faculty of Medicine, Universite Laval, Quebec, Quebec, Canada
    Nat Struct Mol Biol 16:961-6. 2009
    ..Our study lends an additional level of complexity to the control of gene expression in these anucleate elements of the cardiovascular system...
  12. pmc Regulation of human Dicer by the resident ER membrane protein CLIMP-63
    Geneviève Pépin
    CHUQ Research Center CHUL, 2705 Blvd Laurier, QC, G1V 4G2, Canada
    Nucleic Acids Res 40:11603-17. 2012
    ..Our findings define CLIMP-63 as a novel protein interactor and regulator of Dicer function, involved in maintaining Dicer protein levels in human cells...
  13. pmc Detection of human Dicer and Argonaute 2 catalytic activity
    Marjorie P Perron
    CHUL Research Center CHUQ and Faculty of Medicine, Universite Laval, Quebec, QC, Canada
    Methods Mol Biol 725:121-41. 2011
    ..Another protocol aimed at assessing miRNA binding to Ago2 is also described. These experimental approaches are likely to be useful to researchers investigating the main steps of miRNA biogenesis and function in human health and diseases...
  14. pmc Coactosin-like protein supports 5-lipoxygenase enzyme activity and up-regulates leukotriene A4 production
    Marija Rakonjac
    Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry II, Karolinska Institutet, S 171 77 Stockholm, Sweden
    Proc Natl Acad Sci U S A 103:13150-5. 2006
    ..Furthermore, in stimulated cells, CLP appears to function in a complex together with 5LO and membranes, increasing the capacity of 5LO for leukotriene biosynthesis...
  15. pmc Emergence of a complex relationship between HIV-1 and the microRNA pathway
    Dominique L Ouellet
    Centre de Recherche en Rhumatologie et Immunologie, CHUL Research Center, Quebec, Canada
    Methods Mol Biol 487:415-33. 2009
    ..This article summarizes our current state of knowledge on the relationship between HIV-1 and miRNA-guided RNA silencing, and discusses the different aspects of their interaction...
  16. pmc The repertoire and features of human platelet microRNAs
    Helene Plé
    Centre hospitalier universitaire de Québec Research Center Centre hospitalier de l Université Laval, Quebec, Quebec, Canada
    PLoS ONE 7:e50746. 2012
    ....
  17. pmc Involvement of Dcr1 in post-transcriptional regulation of gene expression in Schizosaccharomyces pombe
    Lise Andree Gobeil
    Centre de Recherche en Rhumatologie et Immunologie, Centre de Recherche du CHUL CHUQ, Quebec, QC, G1V 4G2, Canada
    Front Biosci 13:2203-15. 2008
    ..Consistently enriched in Dcr1 immune complexes, Byr3 and L12 may link Dcr1 to the transcriptional and translational machineries, respectively, and contribute to post-transcriptional gene regulation in fission yeast...
  18. doi request reprint Activated platelets can deliver mRNA regulatory Ago2•microRNA complexes to endothelial cells via microparticles
    Benoît Laffont
    Centre hospitalier universitaire de Québec Research Center Centre hospitalier de l Université Laval, Quebec, Canada
    Blood 122:253-61. 2013
    ....
  19. pmc Dicer is required for chromosome segregation and gene silencing in fission yeast cells
    Patrick Provost
    Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry II, Karolinska Institute, S 171 77 Stockholm, Sweden Europe
    Proc Natl Acad Sci U S A 99:16648-53. 2002
    ..Expression profiling demonstrated that dcr1+ was required for silencing of two genes containing a conserved motif...
  20. doi request reprint Dissociation of SERPINE1 mRNA from the translational repressor proteins Ago2 and TIA-1 upon platelet activation
    Aurélie Corduan
    Dr Patrick Provost, CHUQ Research Center CHUL, 2705 Blvd Laurier, Room T1 65, Quebec, QC G1V 4G2, Canada, Tel 1 418 525 4444 ext 48842, E mail
    Thromb Haemost 113:1046-59. 2015
    ....
  21. pmc Alteration of the platelet transcriptome in chronic kidney disease
    Helene Plé
    CHUQ Research Center CHUL, Quebec, Quebec, Canada
    Thromb Haemost 108:605-15. 2012
    ..These results suggest that an alteration of microRNA-based mRNA regulatory mechanisms may underlie the platelet response to uremia and entail the development of platelet-related complications in CKD...
  22. ncbi request reprint Molecular cloning and functional characterization of mouse coactosin-like protein
    Johanne Doucet
    Department of Medical Biochemistry and Biophysics, Karolinska Institute, Stockholm, S 171 77, Sweden
    Biochem Biophys Res Commun 290:783-9. 2002
    ..However, association with actin was best observed at low salt concentrations, while association with 5LO was favored by salt, indicating different binding characteristics...
  23. doi request reprint Platelet microparticles reprogram macrophage gene expression and function
    Benoît Laffont
    Dr Patrick Provost, CHUQ Research Center CHUL, 2705 Blvd Laurier, Room T1 65, Quebec, QC G1V 4G2, Canada, Tel 1 418 525 4444 ext 48842, E mail
    Thromb Haemost 115:311-23. 2016
    ..These findings demonstrate that platelet MPs can modify the transcriptome of macrophages, and reprogram their function towards a phagocytic phenotype. ..
  24. doi request reprint A mutation interfering with 5-lipoxygenase domain interaction leads to increased enzyme activity
    Marija Rakonjac Ryge
    Department of Medical Biochemistry and Biophysics, Division of Physiological Chemistry II, Karolinska Institutet, S 171 77 Stockholm, Sweden
    Arch Biochem Biophys 545:179-85. 2014
    ..The more efficient catalysis following disruption of the R101-D166 ionic association supports the concept of such a flexible lid in human 5-LOX. ..
  25. pmc Protein components of the microRNA pathway and human diseases
    Marjorie P Perron
    Centre de Recherche en Rhumatologie et Immunologie, CHUL Research Center, Quebec, Canada
    Methods Mol Biol 487:369-85. 2009
    ....
  26. pmc MicroRNA-298 and microRNA-328 regulate expression of mouse beta-amyloid precursor protein-converting enzyme 1
    Vincent Boissonneault
    Centre de Recherche en Rhumatologie et Immunologie and Laboratory of Molecular Endocrinology, Centre Hospitalier de l Université Laval Research Center Centre Hospitalier Universitaire de Québec, Quebec, Quebec G1V 4G2, Canada
    J Biol Chem 284:1971-81. 2009
    ..Our results may provide the molecular basis underlying BACE1 deregulation in AD and offer new perspectives on the etiology of this neurological disorder...
  27. pmc A polymerase chain reaction-based cloning strategy applicable to functional microRNA studies
    Vincent Boissonneault
    Centre de Recherche en Rhumatologie et Immunologie, CHUL Research Center, CHUQ, Que, Canada G1V 4G2 Faculty of Medicine, Universite Laval, Que, Canada
    Anal Biochem 381:166-8. 2008
    ....
  28. pmc Protein interactions and complexes in human microRNA biogenesis and function
    Marjorie P Perron
    Centre de Recherche du CHUL CHUQ, 2705 Blvd Laurier, Quebec, QC, G1V 4G2, Canada
    Front Biosci 13:2537-47. 2008
    ....
  29. pmc Revealing the diversity of extracellular vesicles using high-dimensional flow cytometry analyses
    Geneviève Marcoux
    Centre de Recherche du Centre Hospitalier Universitaire de Québec, faculté de médecine de l université Laval, Departement de Microbiologie et Immunologie, Quebec, QC, Canada
    Sci Rep 6:35928. 2016
    ..Our study reveals that computational algorithms are useful for the analysis of high-dimensional single EV data, thereby facilitating comprehension of EV functions and biomarker development...
  30. pmc Dicer-derived microRNAs are utilized by the fragile X mental retardation protein for assembly on target RNAs
    Isabelle Plante
    Centre de Recherche en Rhumatologie et Immunologie, Centre de Recherche du CHUL CHUQ, Sainte Foy, QC, Canada
    J Biomed Biotechnol 2006:64347. 2006
    ..Our results define a possible role for FMRP in RNA silencing and may provide further insight into the molecular defects in patients with the fragile X syndrome...
  31. pmc Current knowledge of MicroRNAs and noncoding RNAs in virus-infected cells
    Dominique L Ouellet
    Centre de Recherche en Rhumatologie et Immunologie, CHUL Research Center CHUQ, Quebec, QC, Canada
    Methods Mol Biol 623:35-65. 2010
    ..This chapter aims to summarize our current knowledge about viral miRNAs/ncRNAs and their targets, as well as cellular miRNAs that are modulated by viruses upon infection...
  32. pmc Platelet microparticles are internalized in neutrophils via the concerted activity of 12-lipoxygenase and secreted phospholipase A2-IIA
    Anne Claire Duchez
    Centre de Recherche du Centre Hospitalier Universitaire de Québec, Departement de Microbiologie et Immunologie, Faculte de Medecine, Universite Laval, Quebec, QC, G1V 4G2 Canada
    Proc Natl Acad Sci U S A 112:E3564-73. 2015
    ....
  33. pmc Regulatory RNAs: future perspectives in diagnosis, prognosis, and individualized therapy
    Marjorie P Perron
    Centre de Recherche en Rhumatologie et Immunologie, Universite Laval, Quebec, Canada
    Methods Mol Biol 361:311-26. 2007
    ..Will bedside miRNA expression profiling eventually assist physicians in providing patients with accurate diagnosis, personalized therapy, and treatment outcome?..
  34. pmc Elevated Abundance, Size, and MicroRNA Content of Plasma Extracellular Vesicles in Viremic HIV-1+ Patients: Correlations With Known Markers of Disease Progression
    Audrey Hubert
    Centre de Recherche du CHU de Québec and Faculty of Medicine, Département de Microbiologie Infectiologie et Immunologie, Universite Laval, Quebec, Quebec, Canada Département des Sciences Biologiques et Centre de recherche BioMed, Universite du Quebec a Montreal UQAM, Montreal, Quebec, Canada Centre de recherche du Centre hospitalier de l Université de Montréal, Département de Microbiologie Infectiologie et Immunologie, Montreal, Quebec, Canada and Chronic Viral Illness Service and Research Institute, McGill University Health Centre, Montreal, Quebec, Canada
    J Acquir Immune Defic Syndr 70:219-27. 2015
    ..EV size, abundance, and content appear to differ according to infection phase, disease progression, and ART...
  35. doi request reprint Dicer cleavage by calpain determines platelet microRNA levels and function in diabetes
    Amro Elgheznawy
    From the Institute for Vascular Signaling, Centre for Molecular Medicine, and DZHK German Centre for Cardiovascular Research partner site Rhine Main, Frankfurt, Germany A E, L S, J H, H L, V R, I F Functional Proteomics, SFB 815 Core Unit, Goethe University, Frankfurt, Germany I W Walter Brendel Centre of Experimental Medicine and DZHK partner site Munich Heart Alliance, Ludwig Maximilians Universitat, Munich, Germany J P Endokrinologikum Frankfurt, Frankfurt, Germany A M and Centre Hospitalier Universitaire de Québec Research Center, and Faculty of Medicine, Universite Laval, Quebec, Canada P P
    Circ Res 117:157-65. 2015
    ..Platelets contain large amounts of miRNA that are altered by disease, in particular diabetes mellitus...
  36. pmc Resistance of human hepatitis delta virus RNAs to dicer activity
    Jinhong Chang
    Fox Chase Cancer Center, Philadelphia, Pennsylvania 19111 2497, USA
    J Virol 77:11910-7. 2003
    ..Apparently, for unit-length RNAs of HDV, PSTVd, and ASBVd, it is the extended structure with <100% base pairing that confers significant resistance to dicer action...
  37. ncbi request reprint The nuclear RNase III Drosha initiates microRNA processing
    Yoontae Lee
    Institute of Molecular Biology and Genetics and School of Biological Sciences, Seoul National University, Seoul 151 742, Korea
    Nature 425:415-9. 2003
    ..Thus, the two RNase III proteins, Drosha and Dicer, may collaborate in the stepwise processing of miRNAs, and have key roles in miRNA-mediated gene regulation in processes such as development and differentiation...
  38. ncbi request reprint NMR structure of human coactosin-like protein
    Edvards Liepinsh
    J Biomol NMR 30:353-6. 2004