I Hromadnikova

Summary

Affiliation: Charles University
Country: Czech Republic

Publications

  1. doi request reprint Gestational hypertension, preeclampsia and intrauterine growth restriction induce dysregulation of cardiovascular and cerebrovascular disease associated microRNAs in maternal whole peripheral blood
    Ilona Hromadnikova
    Department of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University, Prague, Czech Republic Electronic address
    Thromb Res 137:126-40. 2016
  2. doi request reprint Assessment of placental and maternal stress responses in patients with pregnancy related complications via monitoring of heat shock protein mRNA levels
    Ilona Hromadnikova
    Department of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University, Ruska 87, 100 00, Prague, Czech Republic
    Mol Biol Rep 42:625-37. 2015
  3. pmc Extracellular chromosome 21-derived microRNAs in euploid & aneuploid pregnancies
    Katerina Kotlabova
    Department of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University, Prague, Czech Republic
    Indian J Med Res 138:935-43. 2013
  4. pmc Circulating C19MC microRNAs in preeclampsia, gestational hypertension, and fetal growth restriction
    Ilona Hromadnikova
    Department of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University, Ruska 87, 100 00 Prague, Czech Republic
    Mediators Inflamm 2013:186041. 2013
  5. pmc Influence of in vitro IL-2 or IL-15 alone or in combination with Hsp-70-derived 14-mer peptide (TKD) on the expression of NK cell activatory and inhibitory receptors
    Ilona Hromadnikova
    Department of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University in Prague, Ruska 87, 100 00 Prague, Czech Republic
    Mediators Inflamm 2013:405295. 2013
  6. ncbi request reprint [Extracellular chromosome 21--derived microRNAs in maternal circulation: evaluation of their diagnostic potential for screening of Down syndrome]
    I Hromadnikova
    Oddelení molekulární biologie a patologie bunky 3 LF UK
    Ceska Gynekol 77:395-402. 2012
  7. pmc Extracellular nucleic acids in maternal circulation as potential biomarkers for placental insufficiency
    Ilona Hromadnikova
    Department of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University in Prague, Prague 10, Czech Republic
    DNA Cell Biol 31:1221-32. 2012
  8. doi request reprint Absolute and relative quantification of placenta-specific micrornas in maternal circulation with placental insufficiency-related complications
    Ilona Hromadnikova
    Department of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University, Prague, Czech Republic
    J Mol Diagn 14:160-7. 2012
  9. doi request reprint The effect of DYS-14 copy number variations on extracellular fetal DNA quantification in maternal circulation
    Ilona Hromadnikova
    Department of Molecular Biology and Cell Pathology, 3rd Medical Faculty, Charles University, Prague, Czech Republic
    DNA Cell Biol 28:351-8. 2009
  10. doi request reprint Quantification of extracellular DNA using hypermethylated RASSF1A, SRY, and GLO sequences--evaluation of diagnostic possibilities for predicting placental insufficiency
    Ilona Hromadnikova
    Department of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University, Prague, Czech Republic
    DNA Cell Biol 29:295-301. 2010

Detail Information

Publications38

  1. doi request reprint Gestational hypertension, preeclampsia and intrauterine growth restriction induce dysregulation of cardiovascular and cerebrovascular disease associated microRNAs in maternal whole peripheral blood
    Ilona Hromadnikova
    Department of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University, Prague, Czech Republic Electronic address
    Thromb Res 137:126-40. 2016
    ..Initially, selection and validation of endogenous controls for microRNA expression studies in patients affected by pregnancy-related complications have been carried out...
  2. doi request reprint Assessment of placental and maternal stress responses in patients with pregnancy related complications via monitoring of heat shock protein mRNA levels
    Ilona Hromadnikova
    Department of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University, Ruska 87, 100 00, Prague, Czech Republic
    Mol Biol Rep 42:625-37. 2015
    ..Nevertheless, the highest Hsp60 mRNA levels may be observed in pregnancies with signs of the centralization of the fetal circulation associated with fetal hypoxia. ..
  3. pmc Extracellular chromosome 21-derived microRNAs in euploid & aneuploid pregnancies
    Katerina Kotlabova
    Department of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University, Prague, Czech Republic
    Indian J Med Res 138:935-43. 2013
    ....
  4. pmc Circulating C19MC microRNAs in preeclampsia, gestational hypertension, and fetal growth restriction
    Ilona Hromadnikova
    Department of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University, Ruska 87, 100 00 Prague, Czech Republic
    Mediators Inflamm 2013:186041. 2013
    ..The study brought the interesting finding that the upregulation of miR-516-5p, miR-517*, miR-520a*, miR-525, and miR-526a is a characteristic phenomenon of established preeclampsia...
  5. pmc Influence of in vitro IL-2 or IL-15 alone or in combination with Hsp-70-derived 14-mer peptide (TKD) on the expression of NK cell activatory and inhibitory receptors
    Ilona Hromadnikova
    Department of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University in Prague, Ruska 87, 100 00 Prague, Czech Republic
    Mediators Inflamm 2013:405295. 2013
    ..Cell number positive for NKG2C and NKG2A was increased only by IL-2 and IL-2/TKD. The diverse effect of IL-2 or IL-15 w or w/o TKD on cell surface expression was observed in CD16, NKp46, and LIR1/ILT-2...
  6. ncbi request reprint [Extracellular chromosome 21--derived microRNAs in maternal circulation: evaluation of their diagnostic potential for screening of Down syndrome]
    I Hromadnikova
    Oddelení molekulární biologie a patologie bunky 3 LF UK
    Ceska Gynekol 77:395-402. 2012
    ..Subsequently, we studied if plasmatic concentrations and/or expression profile of extracellular chromosome 21-derived microRNAs would distinguish between pregnancies bearing euploid foetuses and those affected with Down syndrome...
  7. pmc Extracellular nucleic acids in maternal circulation as potential biomarkers for placental insufficiency
    Ilona Hromadnikova
    Department of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University in Prague, Prague 10, Czech Republic
    DNA Cell Biol 31:1221-32. 2012
    ....
  8. doi request reprint Absolute and relative quantification of placenta-specific micrornas in maternal circulation with placental insufficiency-related complications
    Ilona Hromadnikova
    Department of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University, Prague, Czech Republic
    J Mol Diagn 14:160-7. 2012
    ..Early gestation extracellular miRNA screening can differentiate between women with normally progressing pregnancies and those who may later develop placental insufficiency-related complications...
  9. doi request reprint The effect of DYS-14 copy number variations on extracellular fetal DNA quantification in maternal circulation
    Ilona Hromadnikova
    Department of Molecular Biology and Cell Pathology, 3rd Medical Faculty, Charles University, Prague, Czech Republic
    DNA Cell Biol 28:351-8. 2009
    ..We concluded that, regarding interindividual copy number variations, the DYS-14 sequence is not an optimal marker for extracellular fetal DNA quantification for differentiation between normal and complicated pregnancies...
  10. doi request reprint Quantification of extracellular DNA using hypermethylated RASSF1A, SRY, and GLO sequences--evaluation of diagnostic possibilities for predicting placental insufficiency
    Ilona Hromadnikova
    Department of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University, Prague, Czech Republic
    DNA Cell Biol 29:295-301. 2010
    ..However, this is strongly individualized, and not a rule for all cases, and probably depends on the actual occurrence of excessive placental trophoblast apoptosis...
  11. doi request reprint Fetal cells of mesenchymal origin in cultures derived from synovial tissue and skin of patients with rheumatoid arthritis
    Ilona Hromadnikova
    Department of Molecular Biology and Cell Pathology, 3rd Medical Faculty, Charles University, Ruska 87, Prague 10, Czech Republic
    Joint Bone Spine 75:563-6. 2008
    ..We conclude that persisting male fetal cells are also able to proliferate in cell culture since their presence was detected even in consecutive passages...
  12. doi request reprint Expression of heat shock protein 70 and NKG2D ligands in acute myeloid leukemia cell lines
    Ilona Hromadnikova
    Department of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University, Prague, Czech Republic
    J Recept Signal Transduct Res 30:161-9. 2010
    ....
  13. doi request reprint Expression of heat shock protein receptors on fibroblast-like synovial cells derived from rheumatoid arthritis-affected joints
    Ilona Hromadnikova
    Department of Molecular Biology and Cell Pathology, 3rd Medical Faculty, Charles University, Ruska 87, Prague 10, 10000, Czech Republic
    Rheumatol Int 28:837-44. 2008
    ..The significance of the Hsp70 interaction with synovial cells via CD91 remains undefined, but may mediate other non-immune purposes...
  14. ncbi request reprint [Detection of placenta-specific microRNAs in maternal circulation]
    I Hromadnikova
    Oddĕlení molekulární biologie a patologie bunky, Gynekologicko porodnická klinika 3 LF UK a FN KV, Praha
    Ceska Gynekol 75:252-6. 2010
    ....
  15. doi request reprint Feasibility of fetal-derived hypermethylated RASSF1A sequence quantification in maternal plasma--next step toward reliable non-invasive prenatal diagnostics
    L Zejskova
    Department of Molecular Biology and Cell Pathology, Third Faculty of Medicine, Charles University, Ruska 87, 100 00 Prague, Czech Republic
    Exp Mol Pathol 89:241-7. 2010
    ....
  16. ncbi request reprint Heat shock protein 70 membrane expression on fibroblast-like synovial cells derived from synovial tissue of patients with rheumatoid and juvenile idiopathic arthritis
    T T H Nguyen
    Cell Biology Laboratory, Department of Paediatrics, University Hospital Motol, 2nd Medical Faculty, Charles University, V Uvalu 84, Prague 5 15006, Czech Republic
    Scand J Rheumatol 35:447-53. 2006
    ..To screen fibroblast-like synovial cells derived from synovial tissue of rheumatoid arthritis (RA) and juvenile idiopathic arthritis (JIA) patients for the membrane expression of the heat shock protein Hsp70...
  17. ncbi request reprint Detection of antibodies against 60-, 65- and 70-kDa heat shock proteins in paediatric patients with various disorders using Western blotting and ELISA
    Thi Thu Hien Nguyen
    Cell Biology Laboratory, Department of Paediatrics, 2nd Medical Faculty and University Hospital Motol, Charles University in Prague, Prague, Czech Republic
    Clin Chem Lab Med 44:442-9. 2006
    ....
  18. ncbi request reprint Frequency of anti-hsp60, -65 and -70 antibodies in sera of patients with juvenile idiopathic arthritis
    Denisa Zlacka
    Cell Biology Laboratory, Department of Paediatrics, University Hospital Motol, 2nd Medical Faculty, Charles University, V Uvalu 84, 15006 Prague 5, Czech Republic
    J Autoimmun 27:81-8. 2006
    ..The prevalence of anti-hsp70 antibodies is much higher in JIA patients when compared with healthy controls, suggesting their possible role in pathological mechanism of the disease...
  19. doi request reprint Heat shock protein gene expression profile may differentiate between rheumatoid arthritis, osteoarthritis, and healthy controls
    L Sedlackova
    Department of Molecular Biology and Cell Pathology, 3rd Faculty of Medicine, Charles University, Prague, Czech Republic
    Scand J Rheumatol 40:354-7. 2011
    ..The aim of this work was to study Hsp mRNA and protein levels to determine whether they can be used to differentiate between RA, osteoarthritis (OA), and healthy controls...
  20. ncbi request reprint In vitro autoreactivity against skin and synovial cells in patients with juvenile idiopathic and rheumatoid arthritis
    K Stechova
    2nd Department of Paediatrics, University Hospital Motol, Prague, Czech Republic
    Pathobiology 70:76-82. 2002
    ..0005) and epidermal keratinocytes (p < 0.0005), however, no difference was found for autologous dermal fibroblasts...
  21. ncbi request reprint Quantification of fetal and total circulatory DNA in maternal plasma samples before and after size fractionation by agarose gel electrophoresis
    I Hromadnikova
    Cell Biology Laboratory, Paediatric Clinic, 2nd Medical Faculty, Charles University, University Hospital Motol, Prague, Czech Republic
    DNA Cell Biol 25:635-40. 2006
    ..However, the usage of size-separated DNA is not suitable for routine clinical applications because of risk of contamination...
  22. ncbi request reprint Non-invasive fetal RHD and RHCE genotyping from maternal plasma in alloimmunized pregnancies
    I Hromadnikova
    Cell Biology Laboratory, Paediatric Clinic, 2nd Medical Faculty, Charles University, University Hospital Motol, Prague, Czech Republic
    Prenat Diagn 25:1079-83. 2005
    ....
  23. ncbi request reprint [A method for modeling skin explants--an in vitro predictive test for graft vs host disease in allogenic hematopoietic cell transplantation]
    I Hromadnikova
    Dĕtská klinika, 2 LF UK a FNM, Praha
    Cas Lek Cesk 140:73-8. 2001
    ..Results GvHD prediction are correlated with the occurrence and severity of acute GvHD posttransplant and effect of GvHD prophylaxis on GvHD clinical outcome is evaluated...
  24. ncbi request reprint In vitro autoreactivity against skin in rheumatoid arthritis: are peripheral blood mononuclear cells of patients with rheumatoid arthritis able to lyze autologous keratinocytes?
    K Stechova
    2nd Department of Pediatrics, University Hospital Motol in Prague, Czech Republic
    Clin Exp Med 1:71-4. 2001
    ..We hypothesize that autologous peripheral blood mononuclear cells might recognize similar autoantigen(s) expressed on epidermal cells, which gives rise to an autoimmune response in the synovium...
  25. ncbi request reprint An in vitro skin explant assay as a predictive assay for graft-versus-host disease in a cohort of pediatric transplants
    I Hromadnikova
    2nd Pediatric Clinic, University Hospital Motol, V Uvalu 84, 150 18 Prague 5, Czech Republic
    Pediatr Transplant 5:258-65. 2001
    ....
  26. doi request reprint Cell surface and relative mRNA expression of heat shock protein 70 in human synovial cells
    Lucie Sedlackova
    Department of Molecular Biology and Cell Pathology, 3rd Medical Faculty, Charles University, Czech Republic
    Autoimmunity 42:17-24. 2009
    ..Heat shock proteins (Hsps) have been repeatedly implicated to participate in the pathogenesis of rheumatoid arthritis (RA)...
  27. doi request reprint Humoral response against Mycobacterium bovis Hsp65 derived fragments in children and young people with various disorders
    Thi Thu Hien Nguyen
    Department of Molecular Biology and Cell Pathology, Charles University in Prague, Prague, Czech Republic
    J Immunoassay Immunochem 29:281-98. 2008
    ..These results suggested that the key B-cell epitopes of MB-Hsp65 might be located on the aforementioned sequences...
  28. doi request reprint Analysis of cell surface and relative gene expression of heat shock protein 70 in human leukemia cell lines
    Ilona Hromadnikova
    Third Medical Faculty, Department of Molecular Biology and Cell Pathology, Charles University, Prague, Czech Republic
    Leuk Lymphoma 49:570-6. 2008
    ..Jurkat cells, however, showed a significant negative correlation between cell surface and mRNA Hsp70 expression; high cell surface expression correlated with low mRNA expression in the Jurkat cell line...
  29. doi request reprint Heat-shock protein expression in leukemia
    Lucie Sedlackova
    Third Faculty of Medicine, Department of Molecular Biology and Cell Pathology, Charles University, Ruska 87, 100 00, Prague 10, Czech Republic
    Tumour Biol 32:33-44. 2011
    ..Leukemia cells showed a heterogeneous pattern of Hsp gene expression among leukemia cell lines as well as in peripheral blood and bone marrow of patients...
  30. ncbi request reprint Antibodies to 60, 65 and 70 kDa heat shock proteins in pediatric allogeneic stem cell transplant recipients
    Denisa Zlacka
    Cell Biology Laboratory, Department of Paediatrics, University Hospital Motol, Charles University, Prague, Czech Republic
    Pediatr Transplant 10:794-804. 2006
    ..We conclude that anti-hsp antibodies might be produced after SCT in relation to infection depending on etiological agents; however, transplant-related complications by themselves had a little impact...
  31. ncbi request reprint Peripheral blood mononuclear cell responses to heat shock proteins in patients undergoing stem cell transplantation
    Lucie Sedlackova
    Department of Pediatrics, Cell Biology Laboratory, 2nd Medical Faculty, Charles University, Prague, Czech Republic
    Pediatr Transplant 10:178-86. 2006
    ..Vice versa, we hypothesize that increased hsp-specific stimulation may reflect the presence of protecting regulatory T cells preventing the development of Th1-mediated diseases involving aGVHD...
  32. ncbi request reprint Non-invasive fetal RHD exon 7 and exon 10 genotyping using real-time PCR testing of fetal DNA in maternal plasma
    Ilona Hromadnikova
    2nd Clinic of Paediatrics, 2nd Medical Faculty, Charles University, University Hospital Motol, Prague, Czech Republic
    Fetal Diagn Ther 20:275-80. 2005
    ....
  33. ncbi request reprint Non-invasive fetal RHD and RHCE genotyping using real-time PCR testing of maternal plasma in RhD-negative pregnancies
    Ilona Hromadnikova
    Clinic of Paediatrics, 2nd Medical Faculty, University Hospital Motol, V Uvalu 84, 150 18 Prague 5, Czech Republic
    J Histochem Cytochem 53:301-5. 2005
    ..In case of D-negative fetus, amplification of another paternally inherited allele (SRY and/or RhC and/or RhE positivity) proves the presence of fetal DNA in maternal circulation...
  34. ncbi request reprint Replicate real-time PCR testing of DNA in maternal plasma increases the sensitivity of non-invasive fetal sex determination
    Ilona Hromadnikova
    2nd Department of Paediatrics, 2nd Medical Faculty, Charles University, University Hospital Motol, V Uvalu 84, 150 18 Prague 5, Czech Republic
    Prenat Diagn 23:235-8. 2003
    ..We determined fetal sex in pregnancies referred for invasive prenatal diagnosis procedures by analysis of DNA in maternal plasma...
  35. ncbi request reprint Anti-cyclic citrullinated peptide antibodies in patients with juvenile idiopathic arthritis
    Ilona Hromadnikova
    2nd Paediatric Clinic, University Hospital Motol, 2nd Medical Faculty, Charles University Prague, Prague, Czech Republic
    Autoimmunity 35:397-401. 2002
    ..Anti-CCP do not have any additional value in MA cohort in comparison to RA where their diagnostic and prognostic importance was reported...
  36. ncbi request reprint Peripheral blood mononuclear cell responses to heat shock proteins and their derived synthetic peptides in juvenile idiopathic arthritis patients
    L Sedlackova
    Cell Biology Laboratory, Department of Paediatrics, 2nd Medical Faculty, Charles University, University Hospital Motol, V Uvalu 84, 155 06 Prague 5, Czech Republic
    Inflamm Res 55:153-9. 2006
    ..Sequence homology and cross reactivity between microbial and human heat shock proteins (hsps) led to the concept that hsps might be involved in the etiopathogenesis of autoimmune diseases...
  37. ncbi request reprint Anti-keratin antibodies in patients with juvenile idiopathic arthritis
    I Hromadnikova
    2nd Paediatric Clinic, University Hospital Motol, Prague, Czech Republic
    Clin Exp Rheumatol 19:470-3. 2001
    ..We discuss the presence of anti-keratin antibodies (AKA) of the IgG class in patients with defined juvenile idiopathic arthritis (JIA)...
  38. ncbi request reprint Analysis of paternal alleles in nucleated red blood cells enriched from maternal blood
    I Hromadnikova
    2nd Clinic of Paediatrics, University Hospital Motol, Prague, Czech Republic
    Folia Biol (Praha) 47:36-9. 2001
    ..Our results demonstrate that foetal cells enriched from maternal peripheral blood may be used as a source of foetal DNA for prenatal diagnosis, paternity testing and other application...