Vladislav Raclavsky

Summary

Affiliation: Palacky University
Location: Olomouc, Czech Republic
Summary:
M.D. in 1990, Ph.D. in 1997, works part-time as principal investigator and part-time as routine mycologist in University Hospital.

My aim is to work for rapid translation of new scientific knowledge into clinical microbiology practice for the benefit of patient. I also strive to supplement this translational research by basic research focused on medically relevant topics in yeasts. Individual topics pursued are as follows:

Hypoxia-response in Cryptococcus neoformans
Ability to adapt to oxygen limitation was identified as a signal for unbudded G2-arrest in this obligate aerobic pathogenic yeast. Furthermore, a role of hypoxia response in cryptococcal virulence has emerged recently. Therefore, we focus on characterisation of cryptococcal hypoxia response useful for development of a cultivating system that should serve as a model for cryptococcal growth in human tissue.

Identification and typing of medically relevant yeasts
Recently, the technology of high resolution melting analysis of double-stranded DNA (typically PCR amplicons) is gradually more affordable to routine clinical microbiology laboratories. Therefore, we focus on developing competitive amplification systems which should best cover the whole spectrum of medically important yeast, enabling for their rapid species identification and possibly also strain typing in a convenient automated or semi-automated way. The McRAPD and PCR-HRMA approaches are at the center of our efforts, including development of multi-purpose software QAS useful for quantitative comparison of complex melting data (melting curves).

Publications

  1. ncbi Deficit in oxygen causes G(2) budding and unbudded G(2) arrest in Cryptococcus neoformans
    M Ohkusu
    Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan
    FEMS Microbiol Lett 204:29-32. 2001
  2. ncbi Preparation and characterization of Cryptococcus neoformans synchronous culture
    Vladislav Raclavsky
    Department of Biology, Faculty of Medicine, Palacky University, Hnevotinska 3, CZ 775 15 Olomouc, Czech Republic
    J Microbiol Methods 51:29-33. 2002
  3. ncbi McRAPD as a new approach to rapid and accurate identification of pathogenic yeasts
    Radek Plachy
    Department of Microbiology, Faculty of Medicine, Palacky University, Hnvotínská 3, CZ 775 15 Olomouc, Czech Republic
    J Microbiol Methods 60:107-13. 2005
  4. ncbi Molecular-genetic approaches to identification and typing of pathogenic Candida yeasts
    Jitka Trtkova
    Department of Biology, Faculty of Medicine, Palacky University, Hnevotinska 3, Olomouc, Czech Republic
    Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 150:51-61. 2006
  5. doi A case of endocarditis caused by the yeast Pichia fabianii with biofilm production and developed in vitro resistance to azoles in the course of antifungal treatment
    Petr Hamal
    Department of Microbiology, University Hospital and Faculty of Medicine, Palacky University Olomouc, Czech Republic
    Med Mycol 46:601-5. 2008
  6. doi Growth strategy of the pathogenic yeast Cryptococcus neoformans submerged culture under different cultivation formats
    V Raclavsky
    Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University, 775 15 Olomouc, Czech Republic
    Folia Microbiol (Praha) 54:349-52. 2009
  7. doi Peculiar clusters of daughter cells observed in Cryptococcus neoformans grown in sealed microtiter plates
    V Raclavsky
    Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University, 775 15 Olomouc, Czech Republic
    Folia Microbiol (Praha) 54:369-71. 2009
  8. ncbi Hypoxia sensing in cryptococcus neoformans: biofilm-like adaptation for dormancy?
    Zuzana Moranova
    Department of Microbiology, Palacky University, Olomouc, Czech Republic
    Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 153:189-93. 2009
  9. pmc Performance of optimized McRAPD in identification of 9 yeast species frequently isolated from patient samples: potential for automation
    Jitka Trtkova
    Department of Microbiology, Palacky University and University Hospital Olomouc, Czech Republic
    BMC Microbiol 9:234. 2009
  10. doi Usefulness of McRAPD for typing and importance of biofilm production in a case of nosocomial ventriculoperitoneal shunt infection caused by Candida lusitaniae
    Petr Hamal
    Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University Olomouc and University Hospital Olomouc, Hnevotinska 3, 77515, Olomouc, Czech Republic
    Folia Microbiol (Praha) 56:407-14. 2011

Collaborators

Detail Information

Publications12

  1. ncbi Deficit in oxygen causes G(2) budding and unbudded G(2) arrest in Cryptococcus neoformans
    M Ohkusu
    Research Center for Pathogenic Fungi and Microbial Toxicoses, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8673, Japan
    FEMS Microbiol Lett 204:29-32. 2001
    ..Thus, a deficit in oxygen was demonstrated to delay the timing of budding, prolong the G(2) phase and cause accumulation of cells after DNA synthesis, but before commitment to budding...
  2. ncbi Preparation and characterization of Cryptococcus neoformans synchronous culture
    Vladislav Raclavsky
    Department of Biology, Faculty of Medicine, Palacky University, Hnevotinska 3, CZ 775 15 Olomouc, Czech Republic
    J Microbiol Methods 51:29-33. 2002
    ..The C. neoformans synchronous culture system has revealed important characteristics with respect to cellular morphology, DNA content and cell volume distribution. The method can be used for further cell cycle studies...
  3. ncbi McRAPD as a new approach to rapid and accurate identification of pathogenic yeasts
    Radek Plachy
    Department of Microbiology, Faculty of Medicine, Palacky University, Hnvotínská 3, CZ 775 15 Olomouc, Czech Republic
    J Microbiol Methods 60:107-13. 2005
    ..We have termed this new approach melting curve of random amplified polymorphic DNA (McRAPD) to emphasize its rapidity and potential for automation, highly desirable features for a routine laboratory test...
  4. ncbi Molecular-genetic approaches to identification and typing of pathogenic Candida yeasts
    Jitka Trtkova
    Department of Biology, Faculty of Medicine, Palacky University, Hnevotinska 3, Olomouc, Czech Republic
    Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 150:51-61. 2006
    ....
  5. doi A case of endocarditis caused by the yeast Pichia fabianii with biofilm production and developed in vitro resistance to azoles in the course of antifungal treatment
    Petr Hamal
    Department of Microbiology, University Hospital and Faculty of Medicine, Palacky University Olomouc, Czech Republic
    Med Mycol 46:601-5. 2008
    ..To our knowledge, this is the first published case of endocarditis and the fourth human infection caused by this yeast species...
  6. doi Growth strategy of the pathogenic yeast Cryptococcus neoformans submerged culture under different cultivation formats
    V Raclavsky
    Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University, 775 15 Olomouc, Czech Republic
    Folia Microbiol (Praha) 54:349-52. 2009
    ....
  7. doi Peculiar clusters of daughter cells observed in Cryptococcus neoformans grown in sealed microtiter plates
    V Raclavsky
    Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University, 775 15 Olomouc, Czech Republic
    Folia Microbiol (Praha) 54:369-71. 2009
    ..However, their daughters were unable to separate from them and to continue their proliferation under such conditions...
  8. ncbi Hypoxia sensing in cryptococcus neoformans: biofilm-like adaptation for dormancy?
    Zuzana Moranova
    Department of Microbiology, Palacky University, Olomouc, Czech Republic
    Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 153:189-93. 2009
    ..During pathogenesis, it relies on well-established virulence factors. This review focuses on the emerging role of cryptococcal adaptation to hypoxia in pathogenesis...
  9. pmc Performance of optimized McRAPD in identification of 9 yeast species frequently isolated from patient samples: potential for automation
    Jitka Trtkova
    Department of Microbiology, Palacky University and University Hospital Olomouc, Czech Republic
    BMC Microbiol 9:234. 2009
    ..Here we have evaluated its performance on a broader spectrum of clinically relevant yeast species and also examined the potential of automated and semi-automated interpretation of McRAPD data for yeast species identification...
  10. doi Usefulness of McRAPD for typing and importance of biofilm production in a case of nosocomial ventriculoperitoneal shunt infection caused by Candida lusitaniae
    Petr Hamal
    Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University Olomouc and University Hospital Olomouc, Hnevotinska 3, 77515, Olomouc, Czech Republic
    Folia Microbiol (Praha) 56:407-14. 2011
    ..A ventriculoperitoneal shunt colonized by C. lusitaniae was revealed as the source of this nosocomial infection, and the ability of the strain to form biofilm on its surface likely caused treatment failure...
  11. doi The potential of high resolution melting analysis (hrma) to streamline, facilitate and enrich routine diagnostics in medical microbiology
    Lenka Ruskova
    Department of Microbiology, Palacky University Olomouc, Czech Republic
    Biomed Pap Med Fac Univ Palacky Olomouc Czech Repub 155:239-52. 2011
    ..This review focuses on the potential of High Resolution Melting Analysis (HRMA) of double-stranded DNA for future routine medical microbiology...
  12. doi NAS agar is more suitable than McKay agar for primary culture of Streptococcus milleri group (SMG) fastidious bacteria, S. intermedius in particular
    Vladislav Raclavsky
    Department of Microbiology, Faculty of Medicine and Dentistry, Palacky University Olomouc, Hnevotinska 3, Olomouc, Czech Republic
    Folia Microbiol (Praha) 62:11-15. 2017
    ..Therefore, when economic conditions are not limiting and maximum recovery rate is desirable, both plates are advised to be used in parallel for primary cultivation of clinical samples...