Andrew L Eamens

Summary

Publications

  1. pmc DRB2, DRB3 and DRB5 function in a non-canonical microRNA pathway in Arabidopsis thaliana
    Andrew L Eamens
    School of Molecular Bioscience, University of Sydney, Sydney, NSW Australia
    Plant Signal Behav 7:1224-9. 2012
  2. pmc DRB2 is required for microRNA biogenesis in Arabidopsis thaliana
    Andrew L Eamens
    School of Molecular Bioscience, University of Sydney, Sydney, New South Wales, Australia
    PLoS ONE 7:e35933. 2012
  3. doi Efficient silencing of endogenous microRNAs using artificial microRNAs in Arabidopsis thaliana
    Andrew L Eamens
    CSIRO Plant Industry, GPO Box 1600, Canberra, ACT 2601, Australia
    Mol Plant 4:157-70. 2011
  4. pmc Alternate approaches to repress endogenous microRNA activity in Arabidopsis thaliana
    Andrew L Eamens
    School of Molecular Bioscience, University of Sydney, Sydney, Australia
    Plant Signal Behav 6:349-59. 2011
  5. doi RNA silencing and antiviral defense in plants
    Claire Agius
    Division of Plant Industry, CSIRO, Canberra, ACT, Australia
    Methods Mol Biol 894:17-38. 2012
  6. pmc The Arabidopsis thaliana double-stranded RNA binding protein DRB1 directs guide strand selection from microRNA duplexes
    Andrew L Eamens
    Commonwealth Scientific and Industrial Research Organisation Plant Industry, Canberra, Australian Capital Territory 2601, Australia
    RNA 15:2219-35. 2009
  7. pmc A 22-nt artificial microRNA mediates widespread RNA silencing in Arabidopsis
    Marcus McHale
    University of Sydney, Waterhouse Laboratory, Lvl 8 Biochemistry Bldg G08, Sydney, NSW, 2006, Australia
    Plant J 76:519-29. 2013
  8. doi The roles of plant dsRNA-binding proteins in RNAi-like pathways
    Shaun J Curtin
    CSIRO Plant Industry Canberra, P O Box 1600, ACT 2601, Australia
    FEBS Lett 582:2753-60. 2008
  9. doi The use of artificial microRNA technology to control gene expression in Arabidopsis thaliana
    Andrew L Eamens
    School of Molecular Sciences, University of Sydney, Sydney, NSW, Australia
    Methods Mol Biol 1062:211-24. 2014
  10. doi In Nicotiana species, an artificial microRNA corresponding to the virulence modulating region of Potato spindle tuber viroid directs RNA silencing of a soluble inorganic pyrophosphatase gene and the development of abnormal phenotypes
    Andrew L Eamens
    CSIRO Plant Industry, Clunies Ross Street, Canberra, ACT 2601, Australia
    Virology 450:266-77. 2014

Collaborators

Detail Information

Publications15

  1. pmc DRB2, DRB3 and DRB5 function in a non-canonical microRNA pathway in Arabidopsis thaliana
    Andrew L Eamens
    School of Molecular Bioscience, University of Sydney, Sydney, NSW Australia
    Plant Signal Behav 7:1224-9. 2012
    ....
  2. pmc DRB2 is required for microRNA biogenesis in Arabidopsis thaliana
    Andrew L Eamens
    School of Molecular Bioscience, University of Sydney, Sydney, New South Wales, Australia
    PLoS ONE 7:e35933. 2012
    ..The importance of miRNA-directed target gene expression in plant development is exemplified by the phenotypic consequence of loss of DRB1 activity (drb1 plants)...
  3. doi Efficient silencing of endogenous microRNAs using artificial microRNAs in Arabidopsis thaliana
    Andrew L Eamens
    CSIRO Plant Industry, GPO Box 1600, Canberra, ACT 2601, Australia
    Mol Plant 4:157-70. 2011
    ..Our findings therefore provide a new and powerful reverse-genetic tool for the analysis of miRNA function in plants...
  4. pmc Alternate approaches to repress endogenous microRNA activity in Arabidopsis thaliana
    Andrew L Eamens
    School of Molecular Bioscience, University of Sydney, Sydney, Australia
    Plant Signal Behav 6:349-59. 2011
    ..All three methodologies rely on endogenous gene regulatory machinery and in this article we provide an overview of these technologies and discuss their strengths and weaknesses in inhibiting the activity of their targeted miRNA(s)...
  5. doi RNA silencing and antiviral defense in plants
    Claire Agius
    Division of Plant Industry, CSIRO, Canberra, ACT, Australia
    Methods Mol Biol 894:17-38. 2012
    ....
  6. pmc The Arabidopsis thaliana double-stranded RNA binding protein DRB1 directs guide strand selection from microRNA duplexes
    Andrew L Eamens
    Commonwealth Scientific and Industrial Research Organisation Plant Industry, Canberra, Australian Capital Territory 2601, Australia
    RNA 15:2219-35. 2009
    ..This suggests that small RNA processing in the DCL1/DRB1-directed miRNA and DCL4/DRB4-directed sRNA biogenesis pathways operates via different mechanisms...
  7. pmc A 22-nt artificial microRNA mediates widespread RNA silencing in Arabidopsis
    Marcus McHale
    University of Sydney, Waterhouse Laboratory, Lvl 8 Biochemistry Bldg G08, Sydney, NSW, 2006, Australia
    Plant J 76:519-29. 2013
    ..Asymmetric 22-nt amiRNA-directed RNA silencing requires little modification of existing amiRNA technology and is expected to be effective in suppressing other genes and/or members of gene families. ..
  8. doi The roles of plant dsRNA-binding proteins in RNAi-like pathways
    Shaun J Curtin
    CSIRO Plant Industry Canberra, P O Box 1600, ACT 2601, Australia
    FEBS Lett 582:2753-60. 2008
    ..DCL2 and DCL3 produce viral siRNAs without requiring assistance from any dsRBP. DRB2, DRB3 and DRB5 appear unnecessary for mi-, tasi-, viral si-, or heterochromatinising siRNA production but act redundantly in a developmental pathway...
  9. doi The use of artificial microRNA technology to control gene expression in Arabidopsis thaliana
    Andrew L Eamens
    School of Molecular Sciences, University of Sydney, Sydney, NSW, Australia
    Methods Mol Biol 1062:211-24. 2014
    ..Here, we describe the rules for amiRNA design, as well as outline the PCR and bacterial cloning procedures involved in the construction of an amiRNA plant expression vector to control target gene expression in Arabidopsis thaliana. ..
  10. doi In Nicotiana species, an artificial microRNA corresponding to the virulence modulating region of Potato spindle tuber viroid directs RNA silencing of a soluble inorganic pyrophosphatase gene and the development of abnormal phenotypes
    Andrew L Eamens
    CSIRO Plant Industry, Clunies Ross Street, Canberra, ACT 2601, Australia
    Virology 450:266-77. 2014
    ..Taken together, our phenotypic and molecular analyses suggest that disease symptom development in Nicotiana species following PSTVd infection results from sRNA-directed RNA silencing of the host gene, siPPase...
  11. pmc The presence of high-molecular-weight viral RNAs interferes with the detection of viral small RNAs
    Neil A Smith
    Comonwealth Scientific and Industrial Research Organization Plant Industry, Canberra, ACT 2601, Australia
    RNA 16:1062-7. 2010
    ..Our findings provide a plausible explanation for the observed strand bias of viral siRNA accumulation, and could have wider implications in the analysis of both viral and nonviral small RNA accumulation...
  12. doi Isolation and detection of small RNAs from plant tissues
    Neil A Smith
    Division of Plant Industry, CSIRO, Canberra, ACT, Australia
    Methods Mol Biol 894:155-72. 2012
    ..Here, we describe the current methods used for the isolation of total RNA and the subsequent enrichment of low-molecular-weight (LMW) RNA species, as well as to outline how sRNAs are detected from such nucleic acid preparations...
  13. pmc Viral small interfering RNAs target host genes to mediate disease symptoms in plants
    Neil A Smith
    CSIRO Plant Industry, Canberra, Australia
    PLoS Pathog 7:e1002022. 2011
    ..Taken together, these findings provide the first demonstration of small RNA-mediated viral disease symptom production and offer an explanation of the species specificity of the viral disease...
  14. doi MicroRNA Regulatory Mechanisms Play Different Roles in Arabidopsis
    Rodrigo S Reis
    School of Biological Sciences, University of Sydney, Macleay Building A12, Sydney, NSW 2006, Australia
    J Proteome Res 14:4743-51. 2015
    ..Enzymes catalyzing each step of this pathway were upregulated in drb1. In contrast, DRB2-associated translational inhibition appears to be less ubiquitous and specifically aimed toward responses against abiotic or biotic stimuli. ..
  15. doi Vectors and methods for hairpin RNA and artificial microRNA-mediated gene silencing in plants
    Andrew L Eamens
    School of Microbial and Molecular Sciences, University of Sydney, Sydney, Australia
    Methods Mol Biol 701:179-97. 2011
    ..In this chapter, we describe the plasmid vector systems routinely used by our research group for the generation of either hpRNA-derived siRNAs or amiRNAs...