Anne Galy

Summary

Country: France

Publications

  1. pmc Development of lentiviral gene therapy for Wiskott Aldrich syndrome
    Anne Galy
    Head of Immunology and Gene Therapy Group, INSERM U790, Genethon, 1 bis rue de l Internationale, 91002 Evry, France
    Expert Opin Biol Ther 8:181-90. 2008
  2. doi request reprint Quantitative proteomic analysis of lentiviral vectors using 2-DE
    Jérome Denard
    Genethon, 91002 Evry, France
    Proteomics 9:3666-76. 2009
  3. pmc Systemic epigenetic response to recombinant lentiviral vectors independent of proviral integration
    Tamás Arányi
    Universite Evry Val d Essonne, UMRS_951, Genethon, 91002 Evry, France
    Epigenetics Chromatin 9:29. 2016
  4. pmc MyD88 signaling in B cells regulates the production of Th1-dependent antibodies to AAV
    Muriel Sudres
    INSERM, U951, Molecular Immunology and Innovative Biotherapies, Genethon, Evry, France
    Mol Ther 20:1571-81. 2012
  5. pmc Quantification of lentiviral vector copy numbers in individual hematopoietic colony-forming cells shows vector dose-dependent effects on the frequency and level of transduction
    S Charrier
    Genethon, Evry, France
    Gene Ther 18:479-87. 2011
  6. pmc Increased production of pro-inflammatory cytokines and enhanced T cell responses after activation of human dendritic cells with IL-1 and CD40 ligand
    Amy Wesa
    Cancer Biology Program and Department of Immunology and Microbiology, Barbara Ann Karmanos Cancer Institute of Wayne State University, Detroit, USA
    BMC Immunol 3:14. 2002
  7. pmc Lentiviral transduction of CD34(+) cells induces genome-wide epigenetic modifications
    Yoshiaki Yamagata
    INSERM, U951, Genethon, Evry, France
    PLoS ONE 7:e48943. 2012
  8. pmc Molecular Determinants of Vectofusin-1 and Its Derivatives for the Enhancement of Lentivirally Mediated Gene Transfer into Hematopoietic Stem/Progenitor Cells
    Saliha Majdoul
    From Généthon, 91000 Evry, France, INSERM UMR_S951, 91000 Evry, France, University of Evry, 91000 Evry, France
    J Biol Chem 291:2161-9. 2016
  9. pmc Peptides derived from evolutionarily conserved domains in Beclin-1 and Beclin-2 enhance the entry of lentiviral vectors into human cells
    Saliha Majdoul
    From Integrare Research Unit, UMR_S951, Genethon, INSERM, University of Evry, EPHE, Evry F 91000, France
    J Biol Chem 292:18672-18681. 2017
  10. doi request reprint Diverse genomic integration of a lentiviral vector developed for the treatment of Wiskott-Aldrich syndrome
    Julie Mantovani
    CNRS UMR 3018, Evry, France
    J Gene Med 11:645-54. 2009

Collaborators

  • A J Thrasher
  • Stuart Ratner
  • William Vainchenker
  • David Fenard
  • Olivier Danos
  • Mary K Collins
  • Jorg Tost
  • Luigi Naldini
  • Otto Wilhelm Merten
  • Burkhard Bechinger
  • Sylvain Fisson
  • Andras Paldi
  • Antoine Kichler
  • Gerben Bouma
  • Siobhan Burns
  • David Alexandre Gross
  • Daniel Scherman
  • Alexis Proust
  • Nathalie Holic
  • Séverine Ciré
  • Laurence Jeanson-Leh
  • Saliha Majdoul
  • Sabine Charrier
  • Sylvie Da Rocha
  • Sophie Frin
  • Ababacar K Seye
  • Tamás Arányi
  • Roseline Yao
  • Daniel Stockholm
  • Catherine Poinsignon
  • Guillaume Corre
  • Nizar Touleimat
  • Madeleine Zerbato
  • Yoshiaki Yamagata
  • Fanny Lafouresse
  • Muriel Sudres
  • S Charrier
  • Javier Perea
  • Julie Mantovani
  • Jérome Denard
  • Abire Jedidi
  • Johanna Blondeau
  • Aurelie Olivier
  • Ababacar Khalil Seye
  • Jérémie Cosette
  • Eric Bernard
  • Laurence Briant
  • Lucile Espert
  • Nathalie Chazal
  • Thibaut Wiart
  • Maxime Ferrand
  • Amy Wesa
  • Christian J Buchholz
  • Michael Blundell
  • Sophie Moniot-Frin
  • Gregory Cédrone
  • Fawzia Louache
  • Dina Ingrao
  • Vinicius Cotta-de-Almeida
  • Gema Malet-Engra
  • Celine Besse
  • Salvatore Valitutti
  • Florence Boisgerault
  • Bernard Ryffel
  • Damien Delafoy
  • Christine Le Bec
  • Veronique Blouin
  • Léa Brault
  • Véronique Parietti
  • Loïc Dupré
  • Virginie Vasseur
  • G Précigout
  • Chrystele Bilhou-Nabera
  • Jean Delaunay
  • S Benkhelifa-Ziyyat
  • Pierre Bordigoni
  • S Bucher-Laurent
  • A Viornery
  • Remi Favier
  • Caroline Deswarte
  • M Zerbato
  • M Ferrand
  • J Perea
  • Ralph Eckenberg
  • Jean Luc Villeval
  • William Saurin
  • Nicole Casadevall
  • Florence Gonnet
  • Jean Antoine Ribeil
  • Nicolas Laroudie

Detail Information

Publications26

  1. pmc Development of lentiviral gene therapy for Wiskott Aldrich syndrome
    Anne Galy
    Head of Immunology and Gene Therapy Group, INSERM U790, Genethon, 1 bis rue de l Internationale, 91002 Evry, France
    Expert Opin Biol Ther 8:181-90. 2008
    ..WAS is attracting growing attention not only because it highlights the rich cellular and systems biology revolving around cytoskeletal regulation but also because it is candidate for a haematopoietic stem cell gene therapy indication...
  2. doi request reprint Quantitative proteomic analysis of lentiviral vectors using 2-DE
    Jérome Denard
    Genethon, 91002 Evry, France
    Proteomics 9:3666-76. 2009
    ..This study defines for the first time, the protein stoichiometry of infectious HIV-1-derived LV particles...
  3. pmc Systemic epigenetic response to recombinant lentiviral vectors independent of proviral integration
    Tamás Arányi
    Universite Evry Val d Essonne, UMRS_951, Genethon, 91002 Evry, France
    Epigenetics Chromatin 9:29. 2016
    ..The effects of LV on target cells are expected to be limited to gene delivery. Yet, human hematopoietic CD34+ cells respond to functional LVs as well as several types of non-integrating LVs by genome-wide DNA methylation changes...
  4. pmc MyD88 signaling in B cells regulates the production of Th1-dependent antibodies to AAV
    Muriel Sudres
    INSERM, U951, Molecular Immunology and Innovative Biotherapies, Genethon, Evry, France
    Mol Ther 20:1571-81. 2012
    ..Therefore, rAAV triggers innate responses, at least via B cells, controlling the development of capsid-specific Th1-driven antibodies. MyD88 emerges as a critical and pivotal regulator of both T- and B-cell adaptive immunity against AAV...
  5. pmc Quantification of lentiviral vector copy numbers in individual hematopoietic colony-forming cells shows vector dose-dependent effects on the frequency and level of transduction
    S Charrier
    Genethon, Evry, France
    Gene Ther 18:479-87. 2011
    ..Thus, the distribution of VCN in hematopoietic colonies may depend upon experimental conditions including features of vectors. This should be carefully evaluated in the context of ex vivo hematopoietic gene therapy studies...
  6. pmc Increased production of pro-inflammatory cytokines and enhanced T cell responses after activation of human dendritic cells with IL-1 and CD40 ligand
    Amy Wesa
    Cancer Biology Program and Department of Immunology and Microbiology, Barbara Ann Karmanos Cancer Institute of Wayne State University, Detroit, USA
    BMC Immunol 3:14. 2002
    ..The immuno-stimulatory capacity of such DC is otherwise not well-defined prompting further characterization of the effects of IL-1 and family members on DC activation in comparison with other pro-inflammatory stimuli...
  7. pmc Lentiviral transduction of CD34(+) cells induces genome-wide epigenetic modifications
    Yoshiaki Yamagata
    INSERM, U951, Genethon, Evry, France
    PLoS ONE 7:e48943. 2012
    ....
  8. pmc Molecular Determinants of Vectofusin-1 and Its Derivatives for the Enhancement of Lentivirally Mediated Gene Transfer into Hematopoietic Stem/Progenitor Cells
    Saliha Majdoul
    From Généthon, 91000 Evry, France, INSERM UMR_S951, 91000 Evry, France, University of Evry, 91000 Evry, France
    J Biol Chem 291:2161-9. 2016
    ..In conclusion, these results help to decipher the action mechanism of Vectofusin-1 in the context of hCD34+ cell-based gene therapy. ..
  9. pmc Peptides derived from evolutionarily conserved domains in Beclin-1 and Beclin-2 enhance the entry of lentiviral vectors into human cells
    Saliha Majdoul
    From Integrare Research Unit, UMR_S951, Genethon, INSERM, University of Evry, EPHE, Evry F 91000, France
    J Biol Chem 292:18672-18681. 2017
    ..Taken together, our findings suggest that the TB1 and TB2 peptides enhance the viral entry step. Tat-Beclin peptides therefore represent a new family of viral transduction enhancers for potential use in gene therapy...
  10. doi request reprint Diverse genomic integration of a lentiviral vector developed for the treatment of Wiskott-Aldrich syndrome
    Julie Mantovani
    CNRS UMR 3018, Evry, France
    J Gene Med 11:645-54. 2009
    ..The genomic integration of a lentiviral vector developed for the treatment of Wiskott-Aldrich syndrome (WAS) was assessed by localizing the vector insertion sites (IS) in a murine model of gene therapy for the disease...
  11. doi request reprint Large-scale manufacture and characterization of a lentiviral vector produced for clinical ex vivo gene therapy application
    Otto Wilhelm Merten
    Genethon, F 91002 Evry, France
    Hum Gene Ther 22:343-56. 2011
    ..Results obtained with the WAS vector provide the initial manufacturing and quality control benchmarking that should be helpful to further development and clinical applications...
  12. pmc The brown algae Pl.LSU/2 group II intron-encoded protein has functional reverse transcriptase and maturase activities
    Madeleine Zerbato
    INSERM, U951 Evry, France
    PLoS ONE 8:e58263. 2013
    ..LSU/2 IEP and the first evidence that the Pl.LSU/2 group II intron splicing occurs in vivo in eukaryotes in an IEP-dependent manner...
  13. ncbi request reprint A partial down-regulation of WASP is sufficient to inhibit podosome formation in dendritic cells
    Aurelie Olivier
    Unité mixte INSERM Université Paris XI, U362, Institut Gustave Roussy, Villejuif F 94805 and CNRS, UMR 8115, Genethon, 91002 Evry, France
    Mol Ther 13:729-37. 2006
    ....
  14. doi request reprint Gene therapy for the Wiskott-Aldrich syndrome
    Anne Galy
    INSERM, U951, Molecular Immunology and Innovative Biotherapies, Genethon, Evry, France
    Curr Opin Allergy Clin Immunol 11:545-50. 2011
    ..One of the first diseases to be successfully treated by allogeneic hematopoietic stem cell transplantation, WAS is currently the subject of several phase I/II gene therapy trials for patients without HLA-compatible donors...
  15. pmc Wiskott-Aldrich syndrome protein-deficient hematopoietic cells can be efficiently mobilized by granulocyte colony-stimulating factor
    Sabine Charrier
    INSERM, U951, Genethon, Evry, France
    Haematologica 98:1300-8. 2013
    ..Thus, in the absence of Wiskott-Aldrich syndrome protein, effective mobilization is achieved but partial correction may occur as a result of an abnormal hematopoietic environment. ..
  16. pmc Vectofusin-1, a new viral entry enhancer, strongly promotes lentiviral transduction of human hematopoietic stem cells
    David Fenard
    1 Genethon, Evry, France 2 INSERM, UMR_S951, Genethon, Evry, France 3 Université Evry Val d Essonne, UMR_S951, Evry, France
    Mol Ther Nucleic Acids 2:e90. 2013
    ..Vectofusin-1 is therefore a promising additive that could significantly ameliorate hCD34(+) cell-based gene therapy.Molecular Therapy-Nucleic Acids (2013) 2, e90; doi:10.1038/mtna.2013.17; published online 7 May 2013...
  17. pmc In Vivo Gene Delivery to Lymph Node Stromal Cells Leads to Transgene-specific CD8+ T Cell Anergy in Mice
    Séverine Ciré
    Research unit UMR_S951, Genethon, INSERM, Univ Evry, EPHE, Evry, France
    Mol Ther 24:1965-1973. 2016
    ..These results open up new perspectives to further characterize lymph node stromal cell functional properties and to develop gene transfer protocols targeting lymph node stroma to induce peripheral tolerance...
  18. doi request reprint The c.273+11dup genetic change in the WAS gene is a functionally neutral polymorphism
    Laurence Jeanson-Leh
    Molecular Immunology and Innovative Biotherapies, INSERM U951, Genethon, Evry, France
    Eur J Haematol 87:366-71. 2011
    ..3%), and eight times in a series of 248 male patients (248 alleles tested: 3.2%). We conclude that the presence of the additional C in the WAS gene is a functionally neutral polymorphism...
  19. doi request reprint Improvement of de novo cholesterol biosynthesis efficiently promotes the production of Human Immunodeficiency Virus type 1-derived lentiviral vectors
    Nathalie Holic
    Genethon, Evry, France
    Hum Gene Ther Methods . 2017
    ....
  20. pmc Wiskott-Aldrich syndrome protein controls antigen-presenting cell-driven CD4+ T-cell motility by regulating adhesion to intercellular adhesion molecule-1
    Fanny Lafouresse
    INSERM, U1043, Toulouse, France
    Immunology 137:183-96. 2012
    ..Our data therefore indicate that WASP controls CD4(+) T-cell motility upon APC encounter by regulating lymphocyte function-associated antigen 1 spatial distribution...
  21. pmc Immunization of mice with lentiviral vectors targeted to MHC class II+ cells is due to preferential transduction of dendritic cells in vivo
    Séverine Ciré
    INSERM, U 951, Molecular Immunology and Innovative Biotherapies, Genethon, Evry, France UMR_S951, University of Evry, Genethon, Evry, France Genethon, Evry, France
    PLoS ONE 9:e101644. 2014
    ....
  22. doi request reprint Selective reduction of JAK2V617F-dependent cell growth by siRNA/shRNA and its reversal by cytokines
    Abire Jedidi
    INSERM, Unité 790, Institut Gustave Roussy, Villejuif, France
    Blood 114:1842-51. 2009
    ..This strongly suggests that patient cytokine levels in current JAK2 inhibitor clinical trials modulate the outcome of these therapies...
  23. doi request reprint Infectivity enhancement of different HIV-1-based lentiviral pseudotypes in presence of the cationic amphipathic peptide LAH4-L1
    David Fenard
    INSERM, UMR_S951, Genethon, Evry F 91002, France
    J Virol Methods 189:375-8. 2013
    ..The action of LAH4-L1 relies both on the GALVTR-LV adhesion and post-adhesion steps. LAH4-L1 represents a new and attractive transduction enhancer for hematopoietic gene therapy protocols...
  24. ncbi request reprint Optimization of short hairpin RNA for lentiviral-mediated RNAi against WAS
    Laurence Jeanson-Leh
    INSERM, U790, Evry F 91002, France Univ Paris Sud, UMR_S790, Orsay Cedex, F 91405 France
    Biochem Biophys Res Commun 362:498-503. 2007
    ..This strategy allowed a strong suppression of WASp in CD34(+) cells and will facilitate future studies on the role of WASp in human cells...
  25. ncbi request reprint Role of Rho-family GTPase Cdc42 in polarized expression of lymphocyte appendages
    Stuart Ratner
    Breast Cancer Research Program and Stem Cell Transplantation Program, Barbara Ann Karmanos Cancer Institute, Detroit, Michigan 48201, USA
    J Leukoc Biol 73:830-40. 2003
    ..The results suggest that one role of Cdc42 in lymphocyte motility is to preserve polarity by concentrating lamellipodial disassembly signals in the uropod...
  26. ncbi request reprint Activated MAPK/ERK kinase (MEK-1) induces transdifferentiation of pigmented epithelium into neural retina
    Anne Galy
    CNRS UMR 146, Institut Curie, Centre Universitaire, Laboratoire 110, 91405 Orsay Cedex, France
    Dev Biol 248:251-64. 2002
    ....