Jorg Tost

Summary

Country: France

Publications

  1. ncbi request reprint DNA methylation analysis by pyrosequencing
    Jorg Tost
    Laboratory for Epigenetics, CEA Institut de Génomique, Centre National de Genotypage, Evry Cedex, France
    Nat Protoc 2:2265-75. 2007
  2. pmc Systemic epigenetic response to recombinant lentiviral vectors independent of proviral integration
    Tamás Arányi
    Universite Evry Val d Essonne, UMRS_951, Genethon, 91002 Evry, France
    Epigenetics Chromatin 9:29. 2016
  3. pmc GFRA3 promoter methylation may be associated with decreased postoperative survival in gastric cancer
    Lars Lohne Eftang
    Department of Clinical Molecular Biology and Laboratory Science EpiGen, Akershus University Hospital, Division of Medicine, Lørenskog, Norway
    BMC Cancer 16:225. 2016
  4. ncbi request reprint Current and Emerging Technologies for the Analysis of the Genome-Wide and Locus-Specific DNA Methylation Patterns
    Jorg Tost
    Laboratory for Epigenetics and Environment, Centre National de Genotypage, CEA Institut de Génomique, 2 rue Gaston Crémieux CP5721, Evry, 91000, France
    Adv Exp Med Biol 945:343-430. 2016
  5. pmc Genome-wide DNA methylation profiles in progression to in situ and invasive carcinoma of the breast with impact on gene transcription and prognosis
    Thomas Fleischer
    Department of Genetics, Institute for Cancer Research, OUS Radiumhospitalet, Montebello, Oslo, 0310, Norway
    Genome Biol 15:435. 2014
  6. pmc No evidence for copy number and methylation variation in H19 and KCNQ10T1 imprinting control regions in children born small for gestational age
    Rinki Murphy
    Department of Medicine, Faculty of Medical and Health Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand
    BMC Med Genet 15:67. 2014
  7. pmc Quantitative DNA methylation analyses reveal stage dependent DNA methylation and association to clinico-pathological factors in breast tumors
    Jovana Klajic
    Department of Clinical Molecular Biology and Laboratory Science EpiGen, Akershus University Hospital, Division of Medicine, 1476 Lørenskog, Norway
    BMC Cancer 13:456. 2013
  8. pmc IGF2/H19 hypomethylation in a patient with very low birthweight, preocious pubarche and insulin resistance
    Rinki Murphy
    Faculty of Medical and Health Sciences, University of Auckland, Auckland, Private Bag 92019, New Zealand
    BMC Med Genet 13:42. 2012
  9. pmc Frequent aberrant DNA methylation of ABCB1, FOXC1, PPP2R2B and PTEN in ductal carcinoma in situ and early invasive breast cancer
    Aslaug Aa Muggerud
    Department of Genetics, Institute for Cancer Research, Oslo University Hospital Radiumhospitalet, Montebello, Oslo, N 0310, Norway
    Breast Cancer Res 12:R3. 2010
  10. pmc Data integration from two microarray platforms identifies bi-allelic genetic inactivation of RIC8A in a breast cancer cell line
    Aslaug Aamodt Muggerud
    Department of Genetics, Institute for Cancer Research, Norwegian Radium Hospital, Oslo University Hospital, 0310 Oslo, Norway
    BMC Med Genomics 2:26. 2009

Collaborators

Detail Information

Publications34

  1. ncbi request reprint DNA methylation analysis by pyrosequencing
    Jorg Tost
    Laboratory for Epigenetics, CEA Institut de Génomique, Centre National de Genotypage, Evry Cedex, France
    Nat Protoc 2:2265-75. 2007
    ..Quantitative epigenotypes are obtained using this protocol in approximately 4 h for up to 96 DNA samples when bisulfite-treated DNA is already available as the starting material...
  2. pmc Systemic epigenetic response to recombinant lentiviral vectors independent of proviral integration
    Tamás Arányi
    Universite Evry Val d Essonne, UMRS_951, Genethon, 91002 Evry, France
    Epigenetics Chromatin 9:29. 2016
    ..The effects of LV on target cells are expected to be limited to gene delivery. Yet, human hematopoietic CD34+ cells respond to functional LVs as well as several types of non-integrating LVs by genome-wide DNA methylation changes...
  3. pmc GFRA3 promoter methylation may be associated with decreased postoperative survival in gastric cancer
    Lars Lohne Eftang
    Department of Clinical Molecular Biology and Laboratory Science EpiGen, Akershus University Hospital, Division of Medicine, Lørenskog, Norway
    BMC Cancer 16:225. 2016
    ..We have compared our findings to the gene expression in the same tissue, and linked our results to prognosis and survival...
  4. ncbi request reprint Current and Emerging Technologies for the Analysis of the Genome-Wide and Locus-Specific DNA Methylation Patterns
    Jorg Tost
    Laboratory for Epigenetics and Environment, Centre National de Genotypage, CEA Institut de Génomique, 2 rue Gaston Crémieux CP5721, Evry, 91000, France
    Adv Exp Med Biol 945:343-430. 2016
    ....
  5. pmc Genome-wide DNA methylation profiles in progression to in situ and invasive carcinoma of the breast with impact on gene transcription and prognosis
    Thomas Fleischer
    Department of Genetics, Institute for Cancer Research, OUS Radiumhospitalet, Montebello, Oslo, 0310, Norway
    Genome Biol 15:435. 2014
    ..DNA methylation alterations are thought to be an early event in progression of cancer, and may prove valuable as a tool in clinical decision making and for understanding neoplastic development...
  6. pmc No evidence for copy number and methylation variation in H19 and KCNQ10T1 imprinting control regions in children born small for gestational age
    Rinki Murphy
    Department of Medicine, Faculty of Medical and Health Sciences, University of Auckland, Private Bag 92019, Auckland, New Zealand
    BMC Med Genet 15:67. 2014
    ..We investigated whether common variation in copy number in the BWS/SRS 11p15 region or altered methylation levels at IGF2/H19 ICR or KCNQ10T1 ICR was associated with SGA...
  7. pmc Quantitative DNA methylation analyses reveal stage dependent DNA methylation and association to clinico-pathological factors in breast tumors
    Jovana Klajic
    Department of Clinical Molecular Biology and Laboratory Science EpiGen, Akershus University Hospital, Division of Medicine, 1476 Lørenskog, Norway
    BMC Cancer 13:456. 2013
    ..In the present study we investigate stage specific changes in the DNA methylation patterns in order to identify valuable markers to understand how these changes affect breast cancer progression...
  8. pmc IGF2/H19 hypomethylation in a patient with very low birthweight, preocious pubarche and insulin resistance
    Rinki Murphy
    Faculty of Medical and Health Sciences, University of Auckland, Auckland, Private Bag 92019, New Zealand
    BMC Med Genet 13:42. 2012
    ....
  9. pmc Frequent aberrant DNA methylation of ABCB1, FOXC1, PPP2R2B and PTEN in ductal carcinoma in situ and early invasive breast cancer
    Aslaug Aa Muggerud
    Department of Genetics, Institute for Cancer Research, Oslo University Hospital Radiumhospitalet, Montebello, Oslo, N 0310, Norway
    Breast Cancer Res 12:R3. 2010
    ....
  10. pmc Data integration from two microarray platforms identifies bi-allelic genetic inactivation of RIC8A in a breast cancer cell line
    Aslaug Aamodt Muggerud
    Department of Genetics, Institute for Cancer Research, Norwegian Radium Hospital, Oslo University Hospital, 0310 Oslo, Norway
    BMC Med Genomics 2:26. 2009
    ..However, subsequent efforts to identify target genes selected for inactivation in these regions have often been challenging...
  11. doi request reprint Methylation of specific CpG sites in the P2 promoter of parathyroid hormone-related protein determines the invasive potential of breast cancer cell lines
    Jorg Tost
    Laboratory for Epigenetics, Centre National de Genotypage, CEA Institut de Génomique Evry, France
    Epigenetics 6:1035-46. 2011
    ..Methylation of the PTHrP P2 is a potential marker of breast cancer progression and might be used to evaluate the metastatic potential of breast tumors...
  12. doi request reprint DNA methylation: an introduction to the biology and the disease-associated changes of a promising biomarker
    Jorg Tost
    Laboratory for Epigenetics, Centre National de Genotypage, CEA Institut de Génomique, 2 rue Gaston Cremieux, 91000 Evry, France
    Mol Biotechnol 44:71-81. 2010
    ..As a stable nucleic-acid-based modification with limited dynamic range that is technically easy to handle, DNA methylation is a promising biomarker for many applications...
  13. doi request reprint DNA methylation: an introduction to the biology and the disease-associated changes of a promising biomarker
    Jorg Tost
    CEA Institut de Génomique, Evry, France
    Methods Mol Biol 507:3-20. 2009
    ..As a stable nucleic acid-based modification with limited dynamic range that is technically easy to handle, DNA methylation is a promising biomarker for many applications...
  14. pmc Non-random, individual-specific methylation profiles are present at the sixth CTCF binding site in the human H19/IGF2 imprinting control region
    Jorg Tost
    Laboratoire d Epigénétique, Centre National de Genotypage, 2 rue Gaston Cremieux, 91000 Evry, France
    Nucleic Acids Res 34:5438-48. 2006
    ..The existence of three individual-specific epigenotypes in the H19 DMR in a non-pathological situation means it is important to reconsider the diagnostic value and functional importance of the sixth CTCF binding site...
  15. ncbi request reprint Serial pyrosequencing for quantitative DNA methylation analysis
    Jorg Tost
    Centre National de Genotypage, Evry, France
    Biotechniques 40:721-2, 724, 726. 2006
  16. doi request reprint Identification and quantification of differentially methylated loci by the pyrosequencing technology
    Emelyne Dejeux
    Laboratory for Epigenetics, Centre National de Genotypage, CEA Institut de Génomique, Evry, France
    Methods Mol Biol 507:189-205. 2009
    ....
  17. ncbi request reprint DNA analysis by mass spectrometry-past, present and future
    Jorg Tost
    Centre National de Genotypage, Bâtiment G2, 2 rue Gaston Cremieux, CP 5721, 91057 Evry Cedex, France
    J Mass Spectrom 41:981-95. 2006
    ..The use of DNA analysis by MS is compared with competing technologies. Finally, an overview is given of worthwhile applications where the know-how gained so far could be used for future developments...
  18. pmc Analysis and accurate quantification of CpG methylation by MALDI mass spectrometry
    Jorg Tost
    Centre National de Genotypage, Bâtiment G2, 2 rue Gaston Cremieux, CP 5721, 91057 Evry Cedex, France
    Nucleic Acids Res 31:e50. 2003
    ..Mass spectrometric results were also compared to chip hybridisation...
  19. doi request reprint Hypermethylation of the IGF2 differentially methylated region 2 is a specific event in insulinomas leading to loss-of-imprinting and overexpression
    Emelyne Dejeux
    Laboratory for Epigenetics CEA Institut de Génomique, Centre National de Genotypage, 91000 Evry, France
    Endocr Relat Cancer 16:939-52. 2009
    ....
  20. pmc Molecular haplotyping at high throughput
    Jorg Tost
    Centre National de Genotypage, Bâtiment G2, 2 rue Gaston Cremieux, CP 5721, 91057 Evry Cedex, France
    Nucleic Acids Res 30:e96. 2002
    ..Molecular haplotyping is illustrated on the well-described beta(2)-adrenergic receptor gene...
  21. ncbi request reprint Analysis and quantification of multiple methylation variable positions in CpG islands by Pyrosequencing
    Jorg Tost
    Centre National de Genotypage, Evry, France
    Biotechniques 35:152-6. 2003
  22. pmc DNA methylation profiling in doxorubicin treated primary locally advanced breast tumours identifies novel genes associated with survival and treatment response
    Emelyne Dejeux
    Laboratory for Epigenetics, Centre National de Genotypage, CEA Institut de Génomique, Evry, France
    Mol Cancer 9:68. 2010
    ..In this study, we aimed to identify DNA methylation based biomarkers to distinguish patients with locally advanced breast cancer who may benefit from neoadjuvant doxorubicin treatment...
  23. ncbi request reprint Analysis of gene-specific DNA methylation patterns by pyrosequencing technology
    Jorg Tost
    Department of Technology Development, Centre National de Genotypage, Evry, France
    Methods Mol Biol 373:89-102. 2007
    ....
  24. doi request reprint DNA methylation profiles distinguish different subtypes of gastroenteropancreatic neuroendocrine tumors
    Alexandre How-Kit
    Laboratory for Epigenetics and Environment, Centre National de Genotypage, CEA Institut de Génomique, Evry, France
    Epigenomics 7:1245-58. 2015
    ..This article investigates if DNA methylation patterns could distinguish subtypes of GEP-NETs...
  25. pmc Rapid identification of promoter hypermethylation in hepatocellular carcinoma by pyrosequencing of etiologically homogeneous sample pools
    Emelyne Dejeux
    Laboratory for Epigenetics, Centre National de Genotypage, Bâtiment G2, 2 rue Gaston Cremieux, CP 5721, 91057 Evry Cedex, France
    J Mol Diagn 9:510-20. 2007
    ..5%, making the method ideally suited for the identification of diagnostic markers based on DNA methylation while saving precious DNA material...
  26. ncbi request reprint Genotyping single nucleotide polymorphisms by MALDI mass spectrometry in clinical applications
    Jorg Tost
    Centre National de Genotypage, Bâtiment G2, 2 rue Gaston Cremieux, CP 5721, 91057 Evry Cedex, France
    Clin Biochem 38:335-50. 2005
    ..Further, it aims to show specialized applications that might lead to diagnostic applications in the future. It does not speculate on whether this methodology will ever reach the diagnostic market...
  27. doi request reprint Complete pipeline for Infinium(®) Human Methylation 450K BeadChip data processing using subset quantile normalization for accurate DNA methylation estimation
    Nizar Touleimat
    Laboratory for Epigenetics, Centre National de Genotypage, CEA Institute de Génomique, Bâtiment G2, 2 rue Gaston Cremieux, Evry, France
    Epigenomics 4:325-41. 2012
    ..However, the assay combines two different assay chemistries, which may cause a bias in the analysis if all signals are merged as a unique source of methylation measurement...
  28. doi request reprint Quality control and single nucleotide resolution analysis of methylated DNA immunoprecipitation products
    Jennifer Sengenès
    Laboratory for Epigenetics, Centre National de Genotypage, CEA Institut de Génomique, 91000 Evry, France
    Anal Biochem 407:141-3. 2010
    ..The presented method can be used as an initial quality measure prior to genome-wide read-out technologies such as microarrays and second-generation sequencing...
  29. pmc Copy number variations alter methylation and parallel IGF2 overexpression in adrenal tumors
    Helene Myrtue Nielsen
    Laboratory for Functional GenomicsFondation Jean Dausset Centre d Etude du Polymorphisme Humain CEPH, Paris, FranceInstitute of BiomedicineAarhus University, Aarhus, DenmarkEndocrine and Metabolic Surgery DepartmentAP HM La Conception, Marseille, FranceDepartment of EndocrinologyAP HM La Timone, Marseille, FranceDepartment of GeneticsInstitute for Cancer Research, Oslo University Hospital, The Norwegian Radium Hospital, Oslo, NorwayDivision of SurgeryTransplantation and Cancer Medicine, Department of Oncology, Oslo University Hospital, Oslo, NorwayThe K G Jebsen Center for Breast Cancer ResearchInstitute for Clinical Medicine, Faculty of Medicine, University of Oslo, Oslo, NorwayPathology DepartmentAP HM La Timone, Marseille, FranceNuclear Endocrine Imaging and Treatment DepartmentAP HM La Timone, Marseille, FranceCancer Research UKLondon Research Institute, London, UKDepartment of Human GeneticsUniversity of Leuven, Leuven, BelgiumGenotyping FacilitiesCentre National de Génotypage, CEA Institut de Génomique, Evry, FranceDepartment of Clinical Molecular Biology EpiGen University of Oslo, Ahus, Lokerod, NorwayLaboratory of Molecular BiologyAP HM La Conception and CRN2M, Aix Marseille University, Marseille, FranceLaboratory for Epigenetics and EnvironmentCentre National de Génotypage, CEA Institut de Génomique, Evry, Germany
    Endocr Relat Cancer 22:953-67. 2015
    ..Our data provide conclusive evidence that loss of the maternal allele correlates with IGF2 overexpression in adrenal tumors and that hypermethylation of the H19 ICR is a consequence thereof. ..
  30. ncbi request reprint Genotyping single nucleotide polymorphisms by mass spectrometry
    Jorg Tost
    Centre National de Genotypage, Bâtiment G2, 2 rue Gaston Cremieux, 91057 Evry Cedex, France
    Mass Spectrom Rev 21:388-418. 2002
    ..Here, the development and history of DNA analysis by mass spectrometry is reviewed and put into the context with the requirements of genomics. All major contributions to the field and their status and limitations are described in detail...
  31. pmc Lentiviral transduction of CD34(+) cells induces genome-wide epigenetic modifications
    Yoshiaki Yamagata
    INSERM, U951, Genethon, Evry, France
    PLoS ONE 7:e48943. 2012
    ....
  32. doi request reprint COLD-PCR Technologies in the Area of Personalized Medicine: Methodology and Applications
    Florence Mauger
    Laboratory for Epigenetics and Environment, Centre National de Genotypage, CEA Institut de Génomique, Bâtiment G2, 2 rue Gaston Cremieux, 91000, Evry, France
    Mol Diagn Ther . 2017
    ..In this review, we describe in detail the different COLD-PCR approaches, highlighting their specificities, advantages, and inconveniences and demonstrating their use in different fields of biological and biomedical research...
  33. doi request reprint SNP-Based Quantification of Allele-Specific DNA Methylation Patterns by Pyrosequencing®
    Florence Busato
    Laboratory for Epigenetics and Environment, Centre National de Genotypage, CEA Institut de Génomique, Bâtiment G2, 2 rue Gaston Cremieux, 91000, Evry, France
    Methods Mol Biol 1315:291-313. 2015
    ....
  34. doi request reprint Quantification of target proteins using hydrogel antibody arrays and MALDI time-of-flight mass spectrometry (A2M2S)
    Ekaterina Darii
    CEA Institut de Génomique, Centre National de Genotypage, Bâtiment G2, 2 rue Gaston Cremieux, 91057 Evry Cedex, France
    N Biotechnol 25:404-16. 2009
    ..It was used to measure the concentration of apolipoprotein A-1 in serum. This method has been termed A2M2S for Affinity Array sand MALDI Mass Spectrometry...