K Becker

Summary

Country: Germany

Publications

  1. pmc Does nasal cocolonization by methicillin-resistant coagulase-negative staphylococci and methicillin-susceptible Staphylococcus aureus strains occur frequently enough to represent a risk of false-positive methicillin-resistant S. aureus determinations by m
    Karsten Becker
    University of Munster, Institute of Medical Microbiology, D 48149 Munster
    J Clin Microbiol 44:229-31. 2006
  2. pmc Kocuria rhizophila adds to the emerging spectrum of micrococcal species involved in human infections
    Karsten Becker
    University Hospital of Munster, Institute of Medical Microbiology, Domagkstr 10, 48149 Munster, Germany
    J Clin Microbiol 46:3537-9. 2008
  3. doi request reprint The clinical impact of livestock-associated methicillin-resistant Staphylococcus aureus of the clonal complex 398 for humans
    Karsten Becker
    Institute of Medical Microbiology, University Hospital of Munster, Munster, Germany Electronic address
    Vet Microbiol . 2015
  4. doi request reprint Staphylococcus aureus from the German general population is highly diverse
    Karsten Becker
    University Hospital Munster, Institute of Medical Microbiology, 48149 Munster, Germany Electronic address
    Int J Med Microbiol 307:21-27. 2017
  5. pmc Detection of mecA- and mecC-Positive Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates by the New Xpert MRSA Gen 3 PCR Assay
    Karsten Becker
    Institute of Medical Microbiology, University Hospital Munster, Munster, Germany
    J Clin Microbiol 54:180-4. 2016
  6. pmc Coagulase-negative staphylococci
    Karsten Becker
    Institute of Medical Microbiology, University Hospital Munster, Munster, Germany
    Clin Microbiol Rev 27:870-926. 2014
  7. doi request reprint Methicillin resistance in Staphylococcus isolates: the "mec alphabet" with specific consideration of mecC, a mec homolog associated with zoonotic S. aureus lineages
    Karsten Becker
    Institute of Medical Microbiology, University Hospital Munster, D 48149 Munster, Germany Electronic address
    Int J Med Microbiol 304:794-804. 2014
  8. pmc Prosthetic valve endocarditis due to Kytococcus schroeteri
    Karsten Becker
    Emerg Infect Dis 9:1493-5. 2003
  9. doi request reprint [Persistent and recurrent skin and soft tissue infections by Staphylococcus aureus. Impact of the small colony-variant (SCV) phenotype and of Panton-Valentine leukocidin (PVL)-positive S. aureus isolates]
    K Becker
    Institut fur Medizinische Mikrobiologie, Universitatsklinikum Munster, Domagkstr 10, 48149, Munster, Deutschland
    Hautarzt 65:15-25. 2014
  10. pmc Evaluation of a modular multiplex-PCR methicillin-resistant Staphylococcus aureus detection assay adapted for mecC detection
    Karsten Becker
    Institute of Medical Microbiology, University Hospital Munster, Munster, Germany
    J Clin Microbiol 51:1917-9. 2013

Detail Information

Publications96

  1. pmc Does nasal cocolonization by methicillin-resistant coagulase-negative staphylococci and methicillin-susceptible Staphylococcus aureus strains occur frequently enough to represent a risk of false-positive methicillin-resistant S. aureus determinations by m
    Karsten Becker
    University of Munster, Institute of Medical Microbiology, D 48149 Munster
    J Clin Microbiol 44:229-31. 2006
    ..aureus-specific gene in parallel and applied directly to clinical specimens would be associated with an unacceptable positive predictive value of about 40%...
  2. pmc Kocuria rhizophila adds to the emerging spectrum of micrococcal species involved in human infections
    Karsten Becker
    University Hospital of Munster, Institute of Medical Microbiology, Domagkstr 10, 48149 Munster, Germany
    J Clin Microbiol 46:3537-9. 2008
    ..K. rhizophila expands the emerging number of "micrococci" considered to be etiologically relevant...
  3. doi request reprint The clinical impact of livestock-associated methicillin-resistant Staphylococcus aureus of the clonal complex 398 for humans
    Karsten Becker
    Institute of Medical Microbiology, University Hospital of Munster, Munster, Germany Electronic address
    Vet Microbiol . 2015
    ..g. younger, shorter length of hospital stay) and clinical characteristics (e.g. less severe complications) which may explain or at least contribute to a lower disease burden of LA-MRSA compared to other MRSA clonal lineages...
  4. doi request reprint Staphylococcus aureus from the German general population is highly diverse
    Karsten Becker
    University Hospital Munster, Institute of Medical Microbiology, 48149 Munster, Germany Electronic address
    Int J Med Microbiol 307:21-27. 2017
    ..This prospective cohort study evaluates colonization dynamics and molecular characteristics of methicillin-susceptible and - resistant Staphylococcus aureus (MSSA/MRSA) in a German general population...
  5. pmc Detection of mecA- and mecC-Positive Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates by the New Xpert MRSA Gen 3 PCR Assay
    Karsten Becker
    Institute of Medical Microbiology, University Hospital Munster, Munster, Germany
    J Clin Microbiol 54:180-4. 2016
    ..All methicillin-susceptible S. aureus strains (n = 98; including staphylococcal cassette chromosome mec element [SCCmec] remnants) and 98.1% of the MRSA strains (n = 160, including 10 mecC-positive MRSA) were accurately categorized. ..
  6. pmc Coagulase-negative staphylococci
    Karsten Becker
    Institute of Medical Microbiology, University Hospital Munster, Munster, Germany
    Clin Microbiol Rev 27:870-926. 2014
    ..In this regard, host susceptibility is much more important. Therapeutically, CoNS are challenging due to the large proportion of methicillin-resistant strains and increasing numbers of isolates with less susceptibility to glycopeptides...
  7. doi request reprint Methicillin resistance in Staphylococcus isolates: the "mec alphabet" with specific consideration of mecC, a mec homolog associated with zoonotic S. aureus lineages
    Karsten Becker
    Institute of Medical Microbiology, University Hospital Munster, D 48149 Munster, Germany Electronic address
    Int J Med Microbiol 304:794-804. 2014
    ....
  8. pmc Prosthetic valve endocarditis due to Kytococcus schroeteri
    Karsten Becker
    Emerg Infect Dis 9:1493-5. 2003
  9. doi request reprint [Persistent and recurrent skin and soft tissue infections by Staphylococcus aureus. Impact of the small colony-variant (SCV) phenotype and of Panton-Valentine leukocidin (PVL)-positive S. aureus isolates]
    K Becker
    Institut fur Medizinische Mikrobiologie, Universitatsklinikum Munster, Domagkstr 10, 48149, Munster, Deutschland
    Hautarzt 65:15-25. 2014
    ..The diagnostic and therapeutic challenges of PVL-positive and SCV isolates necessitate close collaboration with microbiological and infectious disease specialists. ..
  10. pmc Evaluation of a modular multiplex-PCR methicillin-resistant Staphylococcus aureus detection assay adapted for mecC detection
    Karsten Becker
    Institute of Medical Microbiology, University Hospital Munster, Munster, Germany
    J Clin Microbiol 51:1917-9. 2013
    ..aureus (MSSA) isolates. The assay showed 100% sensitivity and specificity for S. aureus species identification as well as for mecA and mecC detection...
  11. doi request reprint [Skin infections with MRSA. Epidemiology and clinical features]
    K Becker
    Institut fur Medizinische Mikrobiologie, Universitatsklinikum Munster, Domagkstr 10, 48149, Munster, Deutschland
    Hautarzt 63:371-80. 2012
    ..CA-MRSA strains often have special virulence factors, such as Panton Valentine leukocidin), and are often associated with specific often recurrent skin and soft tissue infections (furuncles, abscesses, necrotizing entities)...
  12. pmc Anti-inflammatory effects of ciprofloxacin in S. aureus Newman induced nasal inflammation in vitro
    F Sachse
    Department of Otorhinolaryngology Head and Neck Surgery, University of Muenster, Germany
    J Inflamm (Lond) 5:11. 2008
    ..Similar effects have been reported for fluoroquinolones on various cell types. Since these effects have poorly been characterized in CRS, we examined anti-inflammatory effects of ciprofloxacin on human nasal epithelial cells (HNECs)...
  13. pmc Rapid and specific detection of toxigenic Staphylococcus aureus: use of two multiplex PCR enzyme immunoassays for amplification and hybridization of staphylococcal enterotoxin genes, exfoliative toxin genes, and toxic shock syndrome toxin 1 gene
    K Becker
    Institute of Medical Microbiology, University of Munster, 48149 Munster, Germany
    J Clin Microbiol 36:2548-53. 1998
    ..aureus strains for diagnostic purposes as well as research studies...
  14. pmc Development and evaluation of a quality-controlled ribosomal sequence database for 16S ribosomal DNA-based identification of Staphylococcus species
    Karsten Becker
    Institute of Medical Microbiology, University of Munster, D 48149 Munster, Germany
    J Clin Microbiol 42:4988-95. 2004
    ..In contrast to phenotypic methods and the NCBI database, the novel high-quality RIDOM sequence database provides excellent identification of staphylococci, including rarely isolated species and phenotypic variants...
  15. ncbi request reprint Thermonuclease gene as a target for specific identification of Staphylococcus intermedius isolates: use of a PCR-DNA enzyme immunoassay
    Karsten Becker
    Institute of Medical Microbiology, University of Münster Hospital, 48149 Munster, Germany
    Diagn Microbiol Infect Dis 51:237-44. 2005
    ..5%). Except for the limitations with isolates of hoofed animals, the S. intermediusnuc PCR assay has potential for rapid identification of S. intermedius and differentiation from other coagulase-positive staphylococci including S. aureus...
  16. ncbi request reprint Systematic survey on the prevalence of genes coding for staphylococcal enterotoxins SElM, SElO, and SElN
    Karsten Becker
    Institute of Hygiene, University Hospital of Munster, Munster, Germany
    Mol Nutr Food Res 48:488-95. 2004
    ..aureus isolates harbor subsets of pyrogenic toxin superantigens. The potential contribution of seo and sem to the pathogenic potential of S. aureus has to be further evaluated...
  17. pmc Fourier-transform infrared spectroscopic analysis is a powerful tool for studying the dynamic changes in Staphylococcus aureus small-colony variants
    Karsten Becker
    Institute of Medical Microbiology, University Hospital of Munster, D 48149 Munster, Germany
    J Clin Microbiol 44:3274-8. 2006
    ..Thus, FTIR spectroscopy allowed a rapid and reproducible tool for the examination of different subpopulations of S. aureus on solid and in broth media for diagnostic and research purposes...
  18. pmc Systematic survey of nonspecific agglutination by Candida spp. in latex assays
    Karsten Becker
    Institute of Medical Microbiology, University of Munster, D 48149 Munster, Germany
    J Clin Microbiol 45:1315-8. 2007
    ..In fact, 40 (26.7%) of these isolates revealed nonspecific results, significantly associated with the time allowed for agglutination...
  19. pmc Prevalence of genes encoding pyrogenic toxin superantigens and exfoliative toxins among strains of Staphylococcus aureus isolated from blood and nasal specimens
    Karsten Becker
    Institute of Medical Microbiology Institute for Hygiene, University Hospital of Munster, 48149 Munster, Germany
    J Clin Microbiol 41:1434-9. 2003
    ..aureus. Moreover, mainly due to the fixed combinations of seg plus sei, as well as sed plus sej, the possession of multiple PTSAg genes (62.9%) is more frequent than assumed so far...
  20. ncbi request reprint Molecular genotyping of Candida species with special respect to Candida (Torulopsis) glabrata strains by arbitrarily primed PCR
    K Becker
    Institute of Medical Microbiology, University of Munster, Germany
    J Med Microbiol 49:575-81. 2000
    ....
  21. ncbi request reprint Kytococcus schroeteri sp. nov., a novel Gram-positive actinobacterium isolated from a human clinical source
    Karsten Becker
    Institute of Medical Microbiology, University of Munster, Hospital and Clinics, Germany
    Int J Syst Evol Microbiol 52:1609-14. 2002
    ..nov. is proposed. The type strain is strain Muenster 2000T (= DSM 13884T = CCM 4918T)...
  22. pmc Enterotoxigenic potential of Staphylococcus intermedius
    K Becker
    Institute of Medical Microbiology, University of Munster, 48149 Munster, Germany
    Appl Environ Microbiol 67:5551-7. 2001
    ..Since evidence for noninvasive zoonotic transmission of S. intermedius from animal hosts to humans has been documented, an enterotoxigenic role of this microorganism in SFP via contamination of food products may be assumed...
  23. ncbi request reprint [Molecular biological differentiation of yeasts]
    K Becker
    Institut fur Medizinische Mikrobiologie, Westfalische Wilhelms Universitat Munster, Germany
    Mycoses 43:40-7. 2000
    ..Criteria as typeability, reproducibility and discriminatory power should be considered in evaluating typing systems...
  24. ncbi request reprint Survey of staphylococcal enterotoxin genes, exfoliative toxin genes, and toxic shock syndrome toxin 1 gene in non-Staphylococcus aureus species
    K Becker
    Institut fur Medizinische Mikrobiologie, Universitatsklinikum Munster, Germany
    Eur J Clin Microbiol Infect Dis 20:407-9. 2001
    ..The results indicate that the occurrence of toxin genes involved in staphylococcal toxin-mediated diseases is at least very rare in human-derived isolates of non-Staphylococcus aureus species...
  25. pmc Isolation and characterization of a species-specific DNA fragment for identification of Candida (Torulopsis) glabrata by PCR
    K Becker
    Institute of Medical Microbiology, University of Munster, D 48149 Munster, Germany
    J Clin Microbiol 39:3356-9. 2001
    ..glabrata-specific agarose gel bands, which were generated by arbitrarily primed PCR. The expected PCR product was successfully amplified with genomic DNA from 95 C. glabrata isolates but not from a number of other fungal isolates...
  26. ncbi request reprint Proinflammatory impact of Staphylococcus epidermidis on the nasal epithelium quantified by IL-8 and GRO-alpha responses in primary human nasal epithelial cells
    F Sachse
    Department of Otolaryngology, Head and Neck Surgery, University of Munster, Munster, Germany
    Int Arch Allergy Immunol 145:24-32. 2008
    ..Among others, serine and cysteine proteases have been identified as factors of virulence in S. epidermidis...
  27. ncbi request reprint PAR-2 activation regulates IL-8 and GRO-alpha synthesis by NF-kappaB, but not RANTES, IL-6, eotaxin or TARC expression in nasal epithelium
    C Rudack
    Department of Otorhinolaryngology, Head and Neck Surgery, IZK Münster and Boltzmann Institute for Immunobiology of the Skin, Munster, Germany
    Clin Exp Allergy 37:1009-22. 2007
    ..The effects of protease-activated receptor-2 (PAR-2) stimulation on inflammation mechanisms of chronic rhinosinusitis (CRS) are still unknown...
  28. doi request reprint Virulence factors and genotypes of Staphylococcus aureus from infection and carriage in Gabon
    F Schaumburg
    Institute of Medical Microbiology, University Hospital Munster, Munster, Germany
    Clin Microbiol Infect 17:1507-13. 2011
    ..aureus isolates from asymptomatic carriers and infections. Of note, S. aureus isolates from Gabon show a very high prevalence of PVL-encoding genes, which exceeds the rates observed for developed countries...
  29. doi request reprint Cross-border comparison of the admission prevalence and clonal structure of meticillin-resistant Staphylococcus aureus
    R Kock
    Institute of Hygiene, University Hospital Munster, Munster, Germany
    J Hosp Infect 71:320-6. 2009
    ..In patients lacking all risk factors assessed, spa types t011 and t034 were predominant (P<0.001)...
  30. ncbi request reprint Nasal carriage as a source of Staphylococcus aureus bacteremia. Study Group
    C von Eiff
    Institute of Medical Microbiology, University of Munster, Germany
    N Engl J Med 344:11-6. 2001
    ..We examined S. aureus isolates from blood and from nasal specimens to determine whether the organisms in the bloodstream originated from the patient's own flora...
  31. doi request reprint Staphylococcus aureus invades the epithelium in nasal polyposis and induces IL-6 in nasal epithelial cells in vitro
    F Sachse
    Department of Otorhinolaryngology, University Hospital Munster, Munster, Germany
    Allergy 65:1430-7. 2010
    ..Here, we demonstrate S. aureus detection in the mucosa of CRSwNP. In addition, intracellular residency of S. aureus in nasal polyp epithelial cells (NPECs) and its capability to induce TH-2 cytokines were analyzed in vitro...
  32. pmc Prevalence and molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) among pigs on German farms and import of livestock-related MRSA into hospitals
    R Kock
    Institute of Hygiene, University Hospital Munster, Robert Koch Str 41, 48149, Munster, Germany
    Eur J Clin Microbiol Infect Dis 28:1375-82. 2009
    ..Our results indicate that livestock represents a relevant reservoir for the import of MRSA into regional German hospitals...
  33. ncbi request reprint EUREGIO MRSA-net Twente/Münsterland--a Dutch-German cross-border network for the prevention and control of infections caused by methicillin-resistant Staphylococcus aureus
    A W Friedrich
    Institute of Hygiene, University Hospital Munster, Germany
    Euro Surveill 13:. 2008
    ....
  34. ncbi request reprint Reduction of the nosocomial meticillin-resistant Staphylococcus aureus incidence density by a region-wide search and follow-strategy in forty German hospitals of the EUREGIO, 2009 to 2011
    A Jurke
    Department of Infectiology and Hygiene, NRW Centre for Health, Munster, Germany
    Euro Surveill 18:pii=20579. 2013
    ..Subsequently, after two years the nosocomial MRSA-incidence density was reduced. Regional surveillance data, annually provided as benchmarking to the regional hospitals and public health authorities, indicated successful prevention...
  35. doi request reprint Characteristics of hospital patients colonized with livestock-associated meticillin-resistant Staphylococcus aureus (MRSA) CC398 versus other MRSA clones
    R Kock
    Institute of Hygiene, University Hospital Munster, Munster, Germany
    J Hosp Infect 79:292-6. 2011
    ..The differences in patient characteristics could explain, at least in part, the relatively low rates of transmission and infection associated with MRSA CC398 in the healthcare setting...
  36. pmc Analogs of Eap protein are conserved and prevalent in clinical Staphylococcus aureus isolates
    M Hussain
    Institute of Medical Microbiology, University of Muenster, Muenster, Germany
    Clin Diagn Lab Immunol 8:1271-6. 2001
    ..Thus, Map and Eap may provide a potential novel tool for S. aureus identification and typing...
  37. ncbi request reprint Small-colony variants (SCVs) of staphylococci: a role in foreign body-associated infections
    C von Eiff
    Institute of Medical Microbiology, University Hospital of Munster, Munster, Germany
    Int J Artif Organs 30:778-85. 2007
    ....
  38. pmc Identification and characterization of a novel 38.5-kilodalton cell surface protein of Staphylococcus aureus with extended-spectrum binding activity for extracellular matrix and plasma proteins
    M Hussain
    Institute of Medical Microbiology, University Hospital of Muenster, Domagkstrasse 10, 48129 Muenster, Germany
    J Bacteriol 183:6778-86. 2001
    ..In conclusion, the biologic characterization of Emp suggests that it is a member of the group of secreted S. aureus molecules that interact with an extended spectrum of host ligands and thereby contribute to S. aureus pathogenicity...
  39. ncbi request reprint Impact of a molecular approach to improve the microbiological diagnosis of infective heart valve endocarditis
    Claudia Breitkopf
    Institute of Medical Microbiology, University of Münster Hospital, Munster, Germany
    Circulation 111:1415-21. 2005
    ..Despite the use of appropriate laboratory techniques, classic microbiological diagnostics are characterized by a high rate of negative results...
  40. ncbi request reprint Intracellular persistence of Staphylococcus aureus small-colony variants within keratinocytes: a cause for antibiotic treatment failure in a patient with darier's disease
    C von Eiff
    Institute of Medical Microbiology, Westfalische Wilhelms Universitat Munster, Munster, Germany
    Clin Infect Dis 32:1643-7. 2001
    ..The presence of intracellular S. aureus SCVs may protect against host defenses and antibiotic therapy and thus may have contributed to this patient's very prolonged skin infection...
  41. doi request reprint Highly divergent Staphylococcus aureus isolates from African non-human primates
    F Schaumburg
    Institutes of Medical Microbiology Hygiene, University Hospital Munster, Munster, Germany Medical Research Unit, Albert Schweitzer Hospital, Lambarene, Gabon Institute of Tropical Medicine, University Hospital Tubingen, Tubingen, Germany Department of Primatology, Max Planck Institute for Evolutionary Anthropology, Leipzig, Germany Research Group Emerging Zoonoses, Robert Koch Institut, Berlin, Germany
    Environ Microbiol Rep 4:141-6. 2012
    ..These isolates were different from classical human S. aureus strains and mainly derived from monkeys. Our findings provide the basis for future studies addressing the inter- and intra-species transmission of S. aureus in Africa...
  42. ncbi request reprint Methicillin-resistant Staphylococcus aureus (MRSA): burden of disease and control challenges in Europe
    R Kock
    Institute of Hygiene, University Hospital Muenster, Muenster, Germany
    Euro Surveill 15:19688. 2010
    ..Thereby, we aim at identifying unmet needs of surveillance, prevention and control of MRSA in Europe...
  43. pmc Detection of Staphylococcus aureus by 16S rRNA directed in situ hybridisation in a patient with a brain abscess caused by small colony variants
    F Kipp
    Institute of Medical Microbiology, Hospital and Clinics, University of Munster, Germany
    J Neurol Neurosurg Psychiatry 74:1000-2. 2003
    ..Antimicrobial agents such as rifampin which have intracellular activity should be included in treatment of infections caused by S aureus SCV...
  44. doi request reprint In the centre of an epidemic: Fifteen years of LA-MRSA CC398 at the University Hospital Münster
    S van Alen
    Institute of Medical Microbiology, University Hospital Munster, Munster, Germany
    Vet Microbiol . 2016
    ..Overall, LA-MRSA CC398 emerged rapidly during the past decade, developed enormous sublineage diversity and contributed substantially to the total burden of MRSA colonization and infection at the hospital...
  45. doi request reprint Trends in antimicrobial non-susceptibility in methicillin-resistant Staphylococcus aureus from Germany (2004-2011)
    F Schaumburg
    Institute of Medical Microbiology, University Hospital Munster, Munster, Germany
    Clin Microbiol Infect 20:O554-7. 2014
    ..A decrease in non-susceptibility rates for aminoglycosides and quinolones affected all major lineages (spa-clonal complexes 003, 008, and 032). All isolated remained susceptible to glycopeptides and linezolid...
  46. doi request reprint IKK-2 inhibitor TPCA-1 represses nasal epithelial inflammation in vitro
    F Sachse
    Department of Otorhinolaryngology Head and Neck Surgery, University Hospital Munster, Germany
    Rhinology 49:168-73. 2011
    ..Alterations of cellular kinase activities including that of IKK-2 might play a role in this inflammatory process...
  47. ncbi request reprint Falsification of tetrazolium dye (MTT) based cytotoxicity assay results due to mycoplasma contamination of cell cultures
    J Denecke
    University Hospital of Munster, Department of Pediatrics, Munster, Germany
    Anticancer Res 19:1245-8. 1999
    ..Differences decreased with decreasing drug doses and decreasing plated cell count. Our findings confirm the compelling need for periodical mycoplasma screening, especially when tetrazolium based cytotoxicity assay (MTT) are used...
  48. pmc agr-dependent bacterial interference has no impact on long-term colonization of Staphylococcus aureus during persistent airway infection of cystic fibrosis patients
    Barbara C Kahl
    Institute of Medical of Microbiology Institute for Hygiene, University of Muenster, Muenster 48149, Germany
    J Clin Microbiol 41:5199-201. 2003
    ..The success of CF clones in terms of cocolonization and/or infection with S. aureus, prevalence of clones, or persistence appeared to be independent of agr group specificity...
  49. pmc Evaluation of two chromogenic agar media for recovery and identification of Staphylococcus aureus small-colony variants
    Frank Kipp
    Institute of Medical Microbiology, University Hospital of Munster, Domagkstrasse 10, 48149 Munster, Germany
    J Clin Microbiol 43:1956-9. 2005
    ..In conclusion, the most accurate and rapid method to detect both the species S. aureus and the SCV phenotype is to inoculate specimens onto both Columbia blood agar and SAID...
  50. ncbi request reprint Infections associated with medical devices: pathogenesis, management and prophylaxis
    Christof von Eiff
    Institute of Medical Microbiology, University of Münster Hospital and Clinics, Domagkstrasse 10, 48149 Munster, Germany
    Drugs 65:179-214. 2005
    ..Finally, further studies are needed to translate the knowledge on the mechanisms of biofilm formation into applicable therapeutic and preventive strategies...
  51. ncbi request reprint Prevalence of genes encoding for members of the staphylococcal leukotoxin family among clinical isolates of Staphylococcus aureus
    Christof von Eiff
    Institute of Medical Microbiology, University of Munster, Hospital and Clinics, 48149 Munster, Germany
    Diagn Microbiol Infect Dis 49:157-62. 2004
    ..The co-possession of different virulence factors and their probable synergy should receive more attention in order to better understand their role in pathogenicity...
  52. ncbi request reprint Effect of trimethoprim-sulfamethoxazole prophylaxis in AIDS patients on the formation of the small colony variant phenotype of Staphylococcus aureus
    Christof von Eiff
    Institute of Medical Microbiology, University of Munster, Hospital and Clinics, Munster, Germany
    Diagn Microbiol Infect Dis 48:191-4. 2004
    ..Thirty-seven patients (29.6%) harbored S. aureus in their anterior nares, three of these patients (8.1%) had S. aureus SCVs. Interestingly, TMP-SMZ does not appear to select for S. aureus SCVs in nasal swabs of these patients...
  53. doi request reprint High sensitivity detection of the glial fibrillary acidic protein as indicator for TSE risk material in meat products using an immuno-PCR
    Thorsten Kuczius
    Institute for Hygiene, University Hospital Munster, Munster, Germany
    Mol Nutr Food Res 53:1329-35. 2009
    ..It is applicable for use by food and veterinary facilities, and institutes involved in food monitoring...
  54. ncbi request reprint The small colony variant (SCV) concept -- the role of staphylococcal SCVs in persistent infections
    Christof von Eiff
    Institut fur Medizinische Mikrobiologie, Universitatsklinikum Munster, Munster, Germany
    Injury 37:S26-33. 2006
    ..Therefore, when an infection persists for weeks or months or fails to respond to antimicrobial therapy, clinicians as well as laboratory personnel should consider further efforts to search for this staphylococcal subpopulation...
  55. doi request reprint ATIII attenuates endotoxemia induced healing impairment in the colon
    Ricarda Diller
    Department of General Surgery, University Hospital of Munster, Germany
    J Surg Res 157:4-13. 2009
    ..Antithrombin (ATIII) is known to improve the microcirculation in sepsis. We hypothesized that it may also positively influence the healing of the colon anastomoses under endotoxemia...
  56. pmc Comparative in vitro activity of ceftobiprole against staphylococci displaying normal and small-colony variant phenotypes
    Christof von Eiff
    Institute of Medical Microbiology, University of Münster Hospital and Clinics, Germany
    Antimicrob Agents Chemother 49:4372-4. 2005
    ..This compound exhibited an excellent antistaphylococcal activity, comparable to that of linezolid...
  57. pmc Sequencing and staphylococci identification
    Alexander Mellmann
    University Hospital Munster, Munster, Germany
    Emerg Infect Dis 12:333-6. 2006
    ..This database correctly identified 55 clinical staphylococcal isolates; all were correctly identified at the species level. At the subspecies level, rpoB misidentified only 2 isolates...
  58. pmc In vitro activity of recombinant lysostaphin against Staphylococcus aureus isolates from anterior nares and blood
    Christof von Eiff
    Institute of Medical Microbiology, University of Münster Hospital and Clinics, 48149 Muenster, Germany
    Antimicrob Agents Chemother 47:3613-5. 2003
    ..Minimum bactericidal concentrations of 0.16 microg/ml and zones of inhibition ranging between 15 and 21 mm in diameter demonstrate that lysostaphin was highly active against all isolates tested...
  59. pmc Microbiological evaluation of a new growth-based approach for rapid detection of methicillin-resistant Staphylococcus aureus
    Christof von Eiff
    Institute of Medical Microbiology, University Hospital of Munster, Domagkstr 10, 48149 Munster, Germany
    J Antimicrob Chemother 61:1277-80. 2008
    ..To evaluate the reliability of this new assay to successfully detect MRSA strains of different origin and clonality, well-characterized S. aureus strains were tested in this study...
  60. pmc New immuno-PCR assay for detection of low concentrations of shiga toxin 2 and its variants
    Wenlan Zhang
    Institute for Hygiene, University of Munster, Robert Koch Str 41, 48149 Munster, Germany
    J Clin Microbiol 46:1292-7. 2008
    ..It is therefore suitable for use by clinical microbiological laboratories to improve the toxin detection in clinical samples...
  61. pmc eap Gene as novel target for specific identification of Staphylococcus aureus
    Muzaffar Hussain
    Institute of Medical Microbiology, University of Munster, 48149 Munster, Germany
    J Clin Microbiol 46:470-6. 2008
    ..Thus, the unique occurrence of Eap in S. aureus offers a promising tool particularly suitable for molecular diagnostics of this pathogen...
  62. ncbi request reprint A quantitative real-time immuno-PCR approach for detection of staphylococcal enterotoxins
    Andreas Fischer
    Institute of Medical Microbiology, University Hospital of Munster, 48149, Munster, Germany
    J Mol Med (Berl) 85:461-9. 2007
    ....
  63. ncbi request reprint Distribution of capsular and surface polysaccharide serotypes of Staphylococcus aureus
    Christof von Eiff
    Institute of Medical Microbiology, University Hospital of Munster, 48149 Munster, Germany
    Diagn Microbiol Infect Dis 58:297-302. 2007
    ....
  64. ncbi request reprint Bacteria and granulation tissue associated with Montgomery T-tubes
    Frank Schmäl
    Department of Otolarynology, University Hospital Muenster, Germany
    Laryngoscope 113:1394-400. 2003
    ..Although complications (infection, development of granulation tissue) of silicone Montgomery T-tubes have been reported, the microbiological consequences and the origin of granulation tissue have not yet been evaluated...
  65. pmc Phenotype microarray profiling of Staphylococcus aureus menD and hemB mutants with the small-colony-variant phenotype
    Christof von Eiff
    Institute of Medical Microbiology, University Hospital of Munster, Domagkstrasse 10, 48149 Munster, Germany
    J Bacteriol 188:687-93. 2006
    ..Key results of the PM analysis were confirmed in independent growth studies and by using Etest strips for susceptibility testing. PM technology is a new and efficient technology for assessing cellular phenotypes in S. aureus...
  66. pmc Direct blood culturing on solid medium outperforms an automated continuously monitored broth-based blood culture system in terms of time to identification and susceptibility testing
    E A Idelevich
    Institute of Medical Microbiology, University Hospital Munster, Germany
    New Microbes New Infect 10:19-24. 2016
    ..This suggests that the usefulness of direct BC on solid medium should be re-evaluated in the era of rapid microbiology. ..
  67. doi request reprint New epidemiology of Staphylococcus aureus infection in Africa
    F Schaumburg
    Institute of Medical Microbiology, University Hospital Munster, Munster, Germany Centre de Recherches Médicales de Lambaréné, Albert Schweitzer Hospital Lambaréné, Lambarene, Gabon
    Clin Microbiol Infect 20:589-96. 2014
    ..aureus (MRSA) clone USA300, and the dissemination of the typical African clone MRSA sequence type 88...
  68. ncbi request reprint Modern strategies in the prevention of implant-associated infections
    C von Eiff
    Institute of Medical Microbiology, University of Münster Hospital and Clinics, Munster, Germany
    Int J Artif Organs 28:1146-56. 2005
    ..However, future studies are warranted to translate the knowledge on the pathogenesis of device-associated infections into applicable prevention strategies...
  69. ncbi request reprint Emerging Staphylococcus species as new pathogens in implant infections
    C von Eiff
    Institute of Medical Microbiology, University of Münster Hospital and Clinics, Munster, Germany
    Int J Artif Organs 29:360-7. 2006
    ....
  70. doi request reprint Is there any rationale for treatment of Staphylococcus aureus infections with antimicrobials that are determined to be ineffective in vitro?
    A Uekotter
    Institute of Medical Microbiology, University Hospital Munster, Munster, Germany
    Clin Microbiol Infect 17:1142-7. 2011
    ..In a nutshell, no evidence has been published that S. aureus infections can be reliably treated with RDs, either as a single administration or in combination therapy...
  71. pmc Evaluation of different methods to detect methicillin resistance in small-colony variants of Staphylococcus aureus
    Frank Kipp
    Institute of Medical Microbiology, University Hospital of Munster, 48149 Munster, Germany
    J Clin Microbiol 42:1277-9. 2004
    ..Only detection of the mecA gene by PCR and the MRSA-Screen latex agglutination test using a higher colony number were shown to be reliable methods to rapidly detect methicillin resistance in these variants...
  72. ncbi request reprint Staphylococcal chromosomal cassette mec type I, spa type, and expression of Pls are determinants of reduced cellular invasiveness of methicillin-resistant Staphylococcus aureus isolates
    Cornelia Werbick
    Institute of Medical Microbiology, University Hospital of Munster, Germany
    J Infect Dis 195:1678-85. 2007
    ..In contrast, the spa type-deduced multilocus sequence typing clonal complex (MLST-CC) was not able to predict the invasiveness of MRSA strains equally well. The underlying mechanism remains to be elucidated...
  73. ncbi request reprint Methicillin-resistant Staphylococcus aureus keratitis after excimer laser photorefractive keratectomy1
    Werner Förster
    University Eye Hospital, University of Munster, Munster, Germany
    J Cataract Refract Surg 28:722-4. 2002
    ..Cultures were positive for methicillin-resistant Staphylococcus aureus (MRSA). Severe MRSA keratitis is a rare cause of infection after PRK...
  74. pmc Augmented expression of polysaccharide intercellular adhesin in a defined Staphylococcus epidermidis mutant with the small-colony-variant phenotype
    Nahed Al Laham
    Institute of Medical Microbiology, University Hospital of Munster, Domagkstr 10, 48149 Munster, Germany
    J Bacteriol 189:4494-501. 2007
    ..In conclusion, interrupting a hemin biosynthetic gene in S. epidermidis resulted in an SCV phenotype. Markedly larger cell clusters and the ability of the hemB mutant to form a biofilm are related to the augmented expression of PIA...
  75. doi request reprint Immunomodulation of nasal epithelial cells by Staphylococcus aureus-derived serine proteases
    Claudia Rudack
    Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital of Munster, Munster, Germany
    J Immunol 183:7592-601. 2009
    ..S. aureus serine proteases were identified to modulate chemokine synthesis and activate NF-kappaB in nasal epithelial cells, and may therefore be relevant for the pathophysiology of chronic rhinosinusitis...
  76. pmc Persistence of nasal colonization with human pathogenic bacteria and associated antimicrobial resistance in the German general population
    R Kock
    University Hospital Munster, Institute of Medical Microbiology, Munster, Germany
    New Microbes New Infect 9:24-34. 2016
    ..Methicillin-resistant S. aureus carriage was rare but highly associated with occupational livestock contact. ..
  77. doi request reprint Rapid identification of microorganisms from positive blood cultures by MALDI-TOF mass spectrometry subsequent to very short-term incubation on solid medium
    E A Idelevich
    Institute of Medical Microbiology, University Hospital Munster, Munster, Germany
    Clin Microbiol Infect 20:1001-6. 2014
    ..1 h). MALDI-TOF MS using biomass subsequent to very short-term incubation on solid medium allows very early and reliable bacterial identification from positive BCs without additional time and cost expenditure...
  78. pmc Induction of CXC chemokines in A549 airway epithelial cells by trypsin and staphylococcal proteases - a possible route for neutrophilic inflammation in chronic rhinosinusitis
    F Sachse
    Department of Otorhinolaryngology, Head and Neck Surgery, University Hospital Munster, Germany
    Clin Exp Immunol 144:534-42. 2006
    ..Staphylococcal serine proteases induced CXC chemokines in A549 cells, probably by the activation of proteases activated receptors, and thus might potentially be involved in neutrophilic inflammation in chronic sinusitis...
  79. ncbi request reprint Understanding the physiology and adaptation of staphylococci: a post-genomic approach
    Karsten Becker
    Universitat Munster, Institut für Medizinsche Mikrobiologie, Munster, Germany
    Int J Med Microbiol 297:483-501. 2007
    ..aureus and coagulase-negative staphylococci...
  80. ncbi request reprint Real-time quantitative PCR assay for the detection of Helicobacter pylori: no association with sudden infant death syndrome
    Brigitte Loddenkötter
    Institute of Legal Medicine, Munster University Hospital, Röntgenstr 23, 48149, Munster, Germany
    Int J Legal Med 119:202-6. 2005
    ..pylori-specific antibody. Thus, an infection by H. pylori is very rare in cases of SIDS, and thus the postulated association of H. pylori infection and SIDS cannot be confirmed...
  81. pmc Reporter metabolite analysis of transcriptional profiles of a Staphylococcus aureus strain with normal phenotype and its isogenic hemB mutant displaying the small-colony-variant phenotype
    Jochen Seggewiss
    Institute of Medical Microbiology, University Hospital of Munster, Domagkstrasse 10, 48149 Munster, Germany
    J Bacteriol 188:7765-77. 2006
    ..In conclusion, the identified differences may be responsible for the SCV phenotype and its association with chronic and persistent infections...
  82. doi request reprint Staphylococcal strains vary greatly in their ability to induce an inflammatory response in endothelial cells
    Matthias Grundmeier
    Institute of Medical Microbiology, University Hospital of Munster, Domagkstrasse 10, D 48149 Munster, Germany
    J Infect Dis 201:871-80. 2010
    ..Despite good activity of the appropriate antimicrobial substances in vitro, these infections are often difficult to treat, perhaps because of the complex infection process of endovascular tissue...
  83. doi request reprint Incidence of staphylococcal colonization and of the 753Q Toll-like receptor 2 variant in nasal polyposis
    Florian Sachse
    Department of Otorhinolaryngology, Head and Neck Surgery, University of Munster, D 48149 Muenster, Germany
    Am J Rhinol Allergy 24:e10-3. 2010
    ..Because the 753Q TLR2 variant may predispose to staphylococcal infection, we simultaneously analyzed staphylococcal colonization and the R753Q TLR2 single nucleotide polymorphism (SNP) in NP...
  84. doi request reprint Transmission of Staphylococcus aureus between mothers and infants in an African setting
    F Schaumburg
    Institute of Medical Microbiology, University Hospital Munster, Munster, Germany Centre de Recherches Médicales de Lambaréné, Albert Schweitzer Hospital, Lambarene, Gabon
    Clin Microbiol Infect 20:O390-6. 2014
    ..aureus infection control in newborns and infants in an African setting. The detection of USA300-related MRSA fuels the concern about the spread of this clone in Central Africa...
  85. doi request reprint [Systemic therapy with antibiotics. Overview of important antibiotics in dermatology]
    C Sunderkotter
    Klinik für Hautkrankheiten, Universitatsklinikum Munster, Von Esmarch Str 58, 48149, Munster, Deutschland
    Hautarzt 65:113-24. 2014
    ....
  86. doi request reprint [Systemic therapy with anti-infective agents. Principles of rational use of systemic antibiotics in dermatology]
    C Sunderkotter
    Klinik und Poliklinik für Hautkrankheiten, Universitatsklinikum Munster, Von Esmarch Str 58, 48149, Munster, Deutschland
    Hautarzt 65:106-12. 2014
    ..For this reason basic knowledge and special prudence when using antibiotics are just as desirable as an awareness of responsibility for the public welfare...
  87. doi request reprint Identification of differentially expressed small non-protein-coding RNAs in Staphylococcus aureus displaying both the normal and the small-colony variant phenotype
    Luay F Abu-Qatouseh
    University Hospital of Munster, Institute of Medical Microbiology, Domagkstr 10, 48149, Munster, Germany
    J Mol Med (Berl) 88:565-75. 2010
    ..aureus phenotype-specific expression of four novel npcRNAs was documented. Thus, the presence of differentially expressed npcRNAs in S. aureus may help to understand the phenotypic variation and its associated pathogenicity...
  88. pmc In vitro activity against Staphylococcus aureus of a novel antimicrobial agent, PRF-119, a recombinant chimeric bacteriophage endolysin
    Evgeny A Idelevich
    University Hospital of Munster, Institute of Medical Microbiology, D 48149 Munster, Germany
    Antimicrob Agents Chemother 55:4416-9. 2011
    ..391 μg/ml (range, 0.024 to 1.563 μg/ml). All 192 clinical isolates of coagulase-negative staphylococci exhibited MIC values of >50 μg/ml. In conclusion, PRF-119 exhibited very good activity specifically against S. aureus...
  89. doi request reprint Staphylococcus aureus small-colony variants are adapted phenotypes for intracellular persistence
    Lorena Tuchscherr
    Institutes of Medical Microbiology, Center of Clinical Research, University Hospital of Munster, Munster, Germany
    J Infect Dis 202:1031-40. 2010
    ..Thus far, persistent and antibiotic-refractory infections have been largely associated with a subpopulation of S. aureus, the small-colony variants (SCVs)...
  90. pmc Biotyping of enterotoxigenic Staphylococcus aureus by enterotoxin gene cluster (egc) polymorphism and spa typing analyses
    Giuseppe Blaiotta
    Dipartimento di Scienza degli Alimenti, Universita degli Studi di Napoli Federico II, Via Universita, 10080055 Portici Naples, Italy
    Appl Environ Microbiol 72:6117-23. 2006
    ..Hence, the polyphasic approach used in this study can be reliably and advantageously applied for typing egc-positive S. aureus strains...
  91. ncbi request reprint Staphylococcus aureus infection caused by a Panton Valentine leukocidin-producing strain
    Christian Aepinus
    Pediatr Infect Dis J 24:284-5. 2005
  92. ncbi request reprint Staphylococcus pettenkoferi sp. nov., a novel coagulase-negative staphylococcal species isolated from human clinical specimens
    Konrad Trulzsch
    Max von Pettenkofer Institute for Hygiene and Medical Microbiology, University of Munich, Munich, Germany
    Int J Syst Evol Microbiol 57:1543-8. 2007
    ..Due to differences in the results of physiological and chemotaxonomic investigations and DNA-DNA hybridization data, strain A6664 was not included in the description of the novel species...
  93. ncbi request reprint Small colony variants: a pathogenic form of bacteria that facilitates persistent and recurrent infections
    Richard A Proctor
    University of Wisconsin Medical School, 436 SMI, 1300 University Avenue, Madison, Wisconsin 53706, USA
    Nat Rev Microbiol 4:295-305. 2006
    ..This Review covers the phenotypic, genetic and clinical picture associated with small colony variants, with an emphasis on staphylococci, for which the greatest amount of information is available...
  94. ncbi request reprint Intranasal mupirocin to prevent postoperative infections
    Christof von Eiff
    N Engl J Med 347:1207-8; author reply 1207-8. 2002
  95. pmc Staphylococcus lugdunensis pacemaker-related infection
    Harald Seifert
    Institute for Medical Microbiology, Immunology and Hygiene, University of Cologne, Cologne, Germany
    Emerg Infect Dis 11:1283-6. 2005
    ....