H Ulrich Göringer

Summary

Affiliation: Darmstadt University of Technology
Country: Germany

Publications

  1. ncbi request reprint RNA aptamers as potential pharmaceuticals against infections with African trypanosomes
    H U Göringer
    Genetics, Darmstadt University of Technology, Schnittspahnstr 10, 64287 Darmstadt, Germany
    Handb Exp Pharmacol . 2006
  2. doi request reprint 'Gestalt,' composition and function of the Trypanosoma brucei editosome
    H Ulrich Göringer
    Department of Genetics, Darmstadt University of Technology, Germany
    Annu Rev Microbiol 66:65-82. 2012
  3. doi request reprint The structural landscape of native editosomes in African trypanosomes
    H Ulrich Göringer
    Department of Microbiology and Genetics, Darmstadt University of Technology, Darmstadt, Germany
    Wiley Interdiscip Rev RNA 2:395-407. 2011
  4. doi request reprint Parasite-specific aptamers as biosynthetic reagents and potential pharmaceuticals
    H Ulrich Göringer
    Genetics, Darmstadt University of Technology, Schnittspahnstrasse 10, 64287 Darmstadt, Germany
    Trends Parasitol 28:106-13. 2012
  5. ncbi request reprint Serum-stable RNA aptamers to an invariant surface domain of live African trypanosomes
    Matthias Homann
    Institute of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstrasse 10, D 64287 Darmstadt, Germany
    Comb Chem High Throughput Screen 9:491-9. 2006
  6. ncbi request reprint In vitro selection of high-affinity nucleic acid ligands to parasite target molecules
    H Ulrich Göringer
    Department of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstrasse 10, 64287 Darmstadt, Germany
    Int J Parasitol 33:1309-17. 2003
  7. ncbi request reprint TbMP42, a protein component of the RNA editing complex in African trypanosomes, has endo-exoribonuclease activity
    Michael Brecht
    Department of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstrasse 10, 64287 Darmstadt, Germany
    Mol Cell 17:621-30. 2005
  8. pmc Targeting the variable surface of African trypanosomes with variant surface glycoprotein-specific, serum-stable RNA aptamers
    Mihaela Lorger
    Department of Microbiology and Genetics, Darmstadt University of Technology, 64287 Darmstadt, Germany
    Eukaryot Cell 2:84-94. 2003
  9. pmc Kinetoplastid RNA editing involves a 3' nucleotidyl phosphatase activity
    Moritz Niemann
    Genetics, Darmstadt University of Technology, Darmstadt, Germany
    Nucleic Acids Res 37:1897-906. 2009
  10. pmc Trypanosoma brucei 20 S editosomes have one RNA substrate-binding site and execute RNA unwinding activity
    Cordula Böhm
    Department of Molecular Genetics, Darmstadt University of Technology, 64287 Darmstadt, Germany
    J Biol Chem 287:26268-77. 2012

Collaborators

Detail Information

Publications19

  1. ncbi request reprint RNA aptamers as potential pharmaceuticals against infections with African trypanosomes
    H U Göringer
    Genetics, Darmstadt University of Technology, Schnittspahnstr 10, 64287 Darmstadt, Germany
    Handb Exp Pharmacol . 2006
    ..Here we summarize the recent examples of the SELEX technique within the context of identifying high-affinity RNA ligands against the surface of the protozoan parasite Trypanosoma brucei, which is the causative agent of sleeping sickness...
  2. doi request reprint 'Gestalt,' composition and function of the Trypanosoma brucei editosome
    H Ulrich Göringer
    Department of Genetics, Darmstadt University of Technology, Germany
    Annu Rev Microbiol 66:65-82. 2012
    ..Special emphasis is placed on the molecular morphology of the editing complex in order to correlate structural features with functional characteristics...
  3. doi request reprint The structural landscape of native editosomes in African trypanosomes
    H Ulrich Göringer
    Department of Microbiology and Genetics, Darmstadt University of Technology, Darmstadt, Germany
    Wiley Interdiscip Rev RNA 2:395-407. 2011
    ..Here, we summarize the consensus models and structural landmarks of both complexes. We correlate structural features with functional characteristics and provide an outlook into dynamic aspects of the editing reaction cycle...
  4. doi request reprint Parasite-specific aptamers as biosynthetic reagents and potential pharmaceuticals
    H Ulrich Göringer
    Genetics, Darmstadt University of Technology, Schnittspahnstrasse 10, 64287 Darmstadt, Germany
    Trends Parasitol 28:106-13. 2012
    ..Here the published examples of aptamers that target Leishmania-, Trypanosoma- and Plasmodia-specific molecules are reviewed...
  5. ncbi request reprint Serum-stable RNA aptamers to an invariant surface domain of live African trypanosomes
    Matthias Homann
    Institute of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstrasse 10, D 64287 Darmstadt, Germany
    Comb Chem High Throughput Screen 9:491-9. 2006
    ..We demonstrate that antigen-tagged preparations of the aptamer can bind to live trypanosomes and that they can be used to re-direct immunoglobulins to the parasite surface...
  6. ncbi request reprint In vitro selection of high-affinity nucleic acid ligands to parasite target molecules
    H Ulrich Göringer
    Department of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstrasse 10, 64287 Darmstadt, Germany
    Int J Parasitol 33:1309-17. 2003
    ..Here we summarise the recent examples of the SELEX technique within the context of identifying high-affinity ligands against parasite target molecules...
  7. ncbi request reprint TbMP42, a protein component of the RNA editing complex in African trypanosomes, has endo-exoribonuclease activity
    Michael Brecht
    Department of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstrasse 10, 64287 Darmstadt, Germany
    Mol Cell 17:621-30. 2005
    ..However, all three activities can be restored by the addition of rTbMP42. Together, the data suggest that TbMP42 contributes both endo- and exoribonuclease activity to the editing reaction cycle...
  8. pmc Targeting the variable surface of African trypanosomes with variant surface glycoprotein-specific, serum-stable RNA aptamers
    Mihaela Lorger
    Department of Microbiology and Genetics, Darmstadt University of Technology, 64287 Darmstadt, Germany
    Eukaryot Cell 2:84-94. 2003
    ..Aptamers tethered to an antigenic side group are capable of directing antibodies to the surface of the parasite in vitro. In this manner, the RNAs might provide a new strategy for a therapeutic intervention to fight sleeping sickness...
  9. pmc Kinetoplastid RNA editing involves a 3' nucleotidyl phosphatase activity
    Moritz Niemann
    Genetics, Darmstadt University of Technology, Darmstadt, Germany
    Nucleic Acids Res 37:1897-906. 2009
    ..Opposing phosphates at the two pre-mRNA cleavage fragments likely function as a roadblock to prevent premature ligation...
  10. pmc Trypanosoma brucei 20 S editosomes have one RNA substrate-binding site and execute RNA unwinding activity
    Cordula Böhm
    Department of Molecular Genetics, Darmstadt University of Technology, 64287 Darmstadt, Germany
    J Biol Chem 287:26268-77. 2012
    ..Lastly, we demonstrate a so far unknown activity of the editing machinery: editosome-bound RNA becomes unfolded by a chaperone-type RNA unwinding activity...
  11. pmc TbMP42 is a structure-sensitive ribonuclease that likely follows a metal ion catalysis mechanism
    Moritz Niemann
    Genetics, Darmstadt University of Technology, Schnittspahnstrasse 10, 64287 Darmstadt, Germany
    Nucleic Acids Res 36:4465-73. 2008
    ..Together, the data are suggestive of a Zn(2+)-dependent metal ion catalysis mechanism for the ribonucleolytic activity of rTbMP42...
  12. pmc Mechanism of the gBP21-mediated RNA/RNA annealing reaction: matchmaking and charge reduction
    ULRICH F MULLER
    Department of Microbiology and Genetics, Darmstadt University of Technology, Schnittspahnstrasse 10, 64287 Darmstadt, Germany
    Nucleic Acids Res 30:447-55. 2002
    ..As a consequence, the electrostatic repulsion between the two annealing reactants is reduced favoring the hybridization reaction...
  13. ncbi request reprint Post-SELEX chemical optimization of a trypanosome-specific RNA aptamer
    Annette Adler
    Genetics, Darmstadt University of Technology, Schnittspahnstr 10, Darmstadt, Germany
    Comb Chem High Throughput Screen 11:16-23. 2008
    ..Together, the data provide optimized conjugation chemistries for the large-scale production of substituted aptamer 2-16 preparations with improved in vivo functionality...
  14. pmc The RNA chaperone activity of the Trypanosoma brucei editosome raises the dynamic of bound pre-mRNAs
    W Matthias Leeder
    Molecular Genetics, Darmstadt University of Technology, Schnittspahnstraße 10, 64287 Darmstadt, Germany
    Sci Rep 6:19309. 2016
    ..This results in a simplified RNA folding landscape with a reduced energy barrier to facilitate the binding of gRNAs. The data provide a first rational for the enigmatic U-specificity of the editing reaction. ..
  15. doi request reprint RNA helicases involved in U-insertion/deletion-type RNA editing
    Elisabeth Kruse
    Darmstadt University of Technology, Darmstadt, Germany
    Biochim Biophys Acta 1829:835-41. 2013
    ..This article is part of a Special Issue entitled: The Biology of RNA helicases - Modulation for life...
  16. pmc Charge reduction and thermodynamic stabilization of substrate RNAs inhibit RNA editing
    W Matthias Leeder
    Molecular Genetics, Darmstadt University of Technology, Darmstadt, Germany
    PLoS ONE 10:e0118940. 2015
    ..The data shed light on mechanistic details of the editing process and identify critical parameters for the development of new trypanocidal compounds. ..
  17. doi request reprint Three-dimensional reconstruction of Trypanosoma brucei editosomes using single-particle electron microscopy
    H Ulrich Göringer
    Department of Genetics, Darmstadt University of Technology, Darmstadt, Germany
    Methods Mol Biol 718:3-22. 2011
    ..Different sample preparation protocols including negative staining, cryo-negative staining, and unstained cryotechniques as well as the single-particle image processing of electron microscopical images are described...
  18. pmc Multiple G-quartet structures in pre-edited mRNAs suggest evolutionary driving force for RNA editing in trypanosomes
    W Matthias Leeder
    Molecular Genetics, Darmstadt University of Technology, Schnittspahnstraße 10, 64287 Darmstadt, Germany
    Sci Rep 6:29810. 2016
    ..We propose that the different processes have coevolved and suggest the parasite life-cycle and the single mitochondrion as evolutionary driving forces. ..
  19. pmc Molecular crowding inhibits U-insertion/deletion RNA editing in vitro: consequences for the in vivo reaction
    Venkata Subbaraju Katari
    Department of Molecular Genetics, Darmstadt University of Technology, Darmstadt, Germany
    PLoS ONE 8:e83796. 2013
    ..Possible non-diffusional reaction pathways are discussed. ..