Klaus Peter Hunfeld

Summary

Affiliation: Frankfurt am Main
Country: Germany

Publications

  1. ncbi request reprint Antimicrobial susceptibility of Borrelia burgdorferi sensu lato: what we know, what we don't know, and what we need to know
    Klaus Peter Hunfeld
    Institute of Medical Microbiology and Infection Control, University Hospital of Frankfurt, Frankfurt Main, Germany
    Wien Klin Wochenschr 118:659-68. 2006
  2. ncbi request reprint Babesiosis: recent insights into an ancient disease
    K P Hunfeld
    Institute of Medical Microbiology and Infection Control, Hospital of the Johann Wolfgang Goethe University Frankfurt am Main, Department of Serology and Molecular Diagnostics, Frankfurt Main, Germany
    Int J Parasitol 38:1219-37. 2008
  3. pmc Evaluating frequency, diagnostic quality, and cost of Lyme borreliosis testing in Germany: a retrospective model analysis
    I Muller
    Bacteriologic Infection Serology Study Group of Germany BISSGG, Central Laboratory, Institute for Laboratory Medicine, Northwest Medical Centre, Academic Teaching Hospital, Medical Faculty, Johann Wolfgang Goethe University, Steinbacher Hohl 2 26, 60488 Frankfurt am Main, Germany
    Clin Dev Immunol 2012:595427. 2012
  4. ncbi request reprint Prevalence of antibodies against the human granulocytic ehrlichiosis agent in Lyme borreliosis patients from Germany
    K P Hunfeld
    Institute of Medical Microbiology, University Hospital of Frankfurt, Frankfurt Main, Germany
    Eur J Clin Microbiol Infect Dis 18:221-4. 1999
  5. pmc Comparison of in vitro activities of ketolides, macrolides, and an azalide against the spirochete Borrelia burgdorferi
    Klaus Peter Hunfeld
    Institute of Medical Microbiology, University Hospital of Frankfurt, D 60596 Frankfurt Main, Germany
    Antimicrob Agents Chemother 48:344-7. 2004
  6. ncbi request reprint Zoonotic Babesia: possibly emerging pathogens to be considered for tick-infested humans in Central Europe
    K P Hunfeld
    Institute of Medical Microbiology, University Hospital of Frankfurt, Germany
    Int J Med Microbiol 293:93-103. 2004
  7. ncbi request reprint New real-time PCR-based method for in vitro susceptibility testing of Anaplasma phagocytophilum against antimicrobial agents
    Klaus Peter Hunfeld
    Institute of Medical Microbiology, University Hospital of Frankfurt, D 60596 Frankfurt Main, Germany
    Int J Antimicrob Agents 23:563-71. 2004
  8. pmc In vitro susceptibility testing of Borrelia burgdorferi sensu lato isolates cultured from patients with erythema migrans before and after antimicrobial chemotherapy
    Klaus Peter Hunfeld
    Institute of Medical Microbiology, University Hospital of Frankfurt, Paul Ehrlich Str 40, D 60596 Frankfurt Main, Germany
    Antimicrob Agents Chemother 49:1294-301. 2005
  9. ncbi request reprint Risk of culture-confirmed borrelial persistence in patients treated for erythema migrans and possible mechanisms of resistance
    Klaus Peter Hunfeld
    Institute of Medical Microbiology, University Hospital of Frankfurt, Paul Ehrlich Str 40, D 60596 Frankfurt Main, Germany
    Int J Med Microbiol 296:233-41. 2006
  10. ncbi request reprint [Molecular biological detection of pathogens in patients with sepsis. Potentials, limitations and perspectives]
    K P Hunfeld
    Institut für Medizinische Mikrobiologie und Krankenhaushygiene, Klinikum der Johann Wolfgang Goethe Universitat, Paul Ehrlich Str 40, 60596, Frankfurt Main
    Anaesthesist 57:326-37. 2008

Collaborators

Detail Information

Publications33

  1. ncbi request reprint Antimicrobial susceptibility of Borrelia burgdorferi sensu lato: what we know, what we don't know, and what we need to know
    Klaus Peter Hunfeld
    Institute of Medical Microbiology and Infection Control, University Hospital of Frankfurt, Frankfurt Main, Germany
    Wien Klin Wochenschr 118:659-68. 2006
    ..This review will summarize what is and what is not known about antimicrobial resistance in B. burgdorferi s.l. and will discuss open questions that continue to fuel the current debate on treatment-resistant Lyme borreliosis...
  2. ncbi request reprint Babesiosis: recent insights into an ancient disease
    K P Hunfeld
    Institute of Medical Microbiology and Infection Control, Hospital of the Johann Wolfgang Goethe University Frankfurt am Main, Department of Serology and Molecular Diagnostics, Frankfurt Main, Germany
    Int J Parasitol 38:1219-37. 2008
    ..This review covers recent insights into human babesiosis with regard to phylogeny, diagnostics and treatment in order to provide new information on well known as well as recently discovered parasites with zoonotic potential...
  3. pmc Evaluating frequency, diagnostic quality, and cost of Lyme borreliosis testing in Germany: a retrospective model analysis
    I Muller
    Bacteriologic Infection Serology Study Group of Germany BISSGG, Central Laboratory, Institute for Laboratory Medicine, Northwest Medical Centre, Academic Teaching Hospital, Medical Faculty, Johann Wolfgang Goethe University, Steinbacher Hohl 2 26, 60488 Frankfurt am Main, Germany
    Clin Dev Immunol 2012:595427. 2012
    ..Data on the economic impact of Lyme borreliosis (LB) on European health care systems is scarce. This project focused on the epidemiology and costs for laboratory testing in LB patients in Germany...
  4. ncbi request reprint Prevalence of antibodies against the human granulocytic ehrlichiosis agent in Lyme borreliosis patients from Germany
    K P Hunfeld
    Institute of Medical Microbiology, University Hospital of Frankfurt, Frankfurt Main, Germany
    Eur J Clin Microbiol Infect Dis 18:221-4. 1999
    ..6%). The findings indicate that concomitant or serial infections with Borrelia burgdorferi and the HGE agent or closely related organisms may be a common occurrence in tick-exposed patients from Germany...
  5. pmc Comparison of in vitro activities of ketolides, macrolides, and an azalide against the spirochete Borrelia burgdorferi
    Klaus Peter Hunfeld
    Institute of Medical Microbiology, University Hospital of Frankfurt, D 60596 Frankfurt Main, Germany
    Antimicrob Agents Chemother 48:344-7. 2004
    ..0019 micro g/ml) and telithromycin (MIC(90), 0.0078 micro g/ml). Electron-microscope analysis and time-kill studies also supported enhanced effectiveness of both ketolides...
  6. ncbi request reprint Zoonotic Babesia: possibly emerging pathogens to be considered for tick-infested humans in Central Europe
    K P Hunfeld
    Institute of Medical Microbiology, University Hospital of Frankfurt, Germany
    Int J Med Microbiol 293:93-103. 2004
    ....
  7. ncbi request reprint New real-time PCR-based method for in vitro susceptibility testing of Anaplasma phagocytophilum against antimicrobial agents
    Klaus Peter Hunfeld
    Institute of Medical Microbiology, University Hospital of Frankfurt, D 60596 Frankfurt Main, Germany
    Int J Antimicrob Agents 23:563-71. 2004
    ....
  8. pmc In vitro susceptibility testing of Borrelia burgdorferi sensu lato isolates cultured from patients with erythema migrans before and after antimicrobial chemotherapy
    Klaus Peter Hunfeld
    Institute of Medical Microbiology, University Hospital of Frankfurt, Paul Ehrlich Str 40, D 60596 Frankfurt Main, Germany
    Antimicrob Agents Chemother 49:1294-301. 2005
    ..Therefore, resistance mechanisms other than acquired resistance to antimicrobial agents should be considered in patients with LB resistant to treatment...
  9. ncbi request reprint Risk of culture-confirmed borrelial persistence in patients treated for erythema migrans and possible mechanisms of resistance
    Klaus Peter Hunfeld
    Institute of Medical Microbiology, University Hospital of Frankfurt, Paul Ehrlich Str 40, D 60596 Frankfurt Main, Germany
    Int J Med Microbiol 296:233-41. 2006
    ..Borrelial persistence, however, is unlikely to result from acquired resistance against antimicrobial agents that were used for initial specific chemotherapy...
  10. ncbi request reprint [Molecular biological detection of pathogens in patients with sepsis. Potentials, limitations and perspectives]
    K P Hunfeld
    Institut für Medizinische Mikrobiologie und Krankenhaushygiene, Klinikum der Johann Wolfgang Goethe Universitat, Paul Ehrlich Str 40, 60596, Frankfurt Main
    Anaesthesist 57:326-37. 2008
    ....
  11. ncbi request reprint [Evidence-based antibiotic prophylaxis in aseptic orthopedic surgery]
    K P Hunfeld
    Institut fur Medizinische Mikrobiologie, Klinikum, Johann Wolfgang Goethe Universitat, Frankfurt M
    Orthopade 32:1070-7. 2003
    ..Antibiotic administration may be repeated in the OR when surgery lasts longer than 3 h. The use of local antibiotics in bone cement has not proven useful as a prophylactic measure...
  12. ncbi request reprint Risk of Pseudomonas aeruginosa cross-colonisation in patients with cystic fibrosis within a holiday camp--a molecular-epidemiological study
    K P Hunfeld
    Institute of Medical Microbiology, Frankfurt University Clinic, Frankfurt Main, Federal Republic of Germany
    Wien Klin Wochenschr 112:329-33. 2000
    ..The aim is to provide an answer to the question as to whether or not social contact outside the hospital environment involves a potential risk for person-to-person spread of this pathogen...
  13. ncbi request reprint Colorimetric in vitro susceptibility testing of penicillins, cephalosporins, macrolides, streptogramins, tetracyclines, and aminoglycosides against Borrelia burgdorferi isolates
    K P Hunfeld
    Institute of Medical Microbiology, University Hospital of Frankfurt, Paul Ehrlich Str 40, D 60596, Frankfurt Main, Germany
    Int J Antimicrob Agents 15:11-7. 2000
    ..Our study showed excellent in vitro antimicrobial activity of all classes of antibiotics tested, except the aminoglycosides and hence their suitability for therapy of Lyme disease...
  14. ncbi request reprint In vitro activity of mezlocillin, meropenem, aztreonam, vancomycin, teicoplanin, ribostamycin and fusidic acid against Borrelia burgdorferi
    K P Hunfeld
    Institute of Medical Microbiology, University Hospital of Frankfurt, Paul Ehrlich Strasse 40, 60596 Frankfurt Main, Germany
    Int J Antimicrob Agents 17:203-8. 2001
    ..burgdorferi complex. The excellent in vitro effectiveness of acylamino-penicillin derivatives and their suitability for the therapy of Lyme disease is emphasised...
  15. ncbi request reprint New colorimetric microdilution method for in vitro susceptibility testing of Borrelia burgdorferi against antimicrobial substances
    K P Hunfeld
    Institute of Medical Microbiology, University Hospital of Frankfurt, Frankfurt Main, Germany
    Eur J Clin Microbiol Infect Dis 19:27-32. 2000
    ..The colorimetric microdilution method offered the advantages of reliability, reproducibility, and convenience and could handle large numbers of isolates and antibiotics...
  16. pmc Seroprevalence of Babesia infections in humans exposed to ticks in midwestern Germany
    Klaus Peter Hunfeld
    Institute of Medical Microbiology, University Hospital of Frankfurt, D 60596 Frankfurt Main, Germany
    J Clin Microbiol 40:2431-6. 2002
    ....
  17. ncbi request reprint Standardised in vitro susceptibility testing of Borrelia burgdorferi against well-known and newly developed antimicrobial agents--possible implications for new therapeutic approaches to Lyme disease
    Klaus Peter Hunfeld
    Institute of Medical Microbiology, University Hospital of Frankfurt, Germany
    Int J Med Microbiol 291:125-37. 2002
    ....
  18. ncbi request reprint Development and laboratory evaluation of a new recombinant ELISA for the serodiagnosis of Lyme disease
    Klaus Peter Hunfeld
    Institute of Medical Microbiology, University Hospital of Frankfurt Main, Germany
    Wien Klin Wochenschr 114:580-5. 2002
    ..The aim of the present study was to develop and evaluate a new recombinant ELISA (RE) for the detection of specific IgG and IgM antibodies against Borrelia burgdorferi...
  19. ncbi request reprint Quality of Lyme disease serology. Lessons from the German Proficiency Testing Program 1999-2001. A preliminary report
    Klaus Peter Hunfeld
    Central Laboratory of the German Proficiency Testing Program for Bacteriological Infection Serology, Institute of Medical Microbiology, University Hospital of Frankfurt Main, Germany
    Wien Klin Wochenschr 114:591-600. 2002
    ..We report on the results of a Lyme disease proficiency testing program which is regularly organised twice a year by our institutions in close cooperation with the Institute of Standardisation in the Medical Laboratory (INSTAND)...
  20. ncbi request reprint In vitro activity of eight oral cephalosporins against Borrelia burgdorferi
    Klaus Peter Hunfeld
    Institute of Medical Microbiology, University Hospital of Frankfurt, Paul Ehrlich Street 40, D 60596 Frankfurt Main, Germany
    Int J Antimicrob Agents 21:313-8. 2003
    ..Our study demonstrates the superior in vitro effectiveness of ceftriaxone with good to excellent activity with the oral agents cefuroxime-axetil, cefixime and cefdinir against B. burgdorferi under strictly standardised test conditions...
  21. pmc Is serological testing a reliable tool in laboratory diagnosis of syphilis? Meta-analysis of eight external quality control surveys performed by the german infection serology proficiency testing program
    Iris Muller
    Institute of Medical Microbiology, University Hospital of Frankfurt, Paul Ehrlich Str 40, D 60596 Frankfurt Main, Germany
    J Clin Microbiol 44:1335-41. 2006
    ..Consequently, the continuous participation of laboratories in proficiency testing and further standardization of tests is strongly recommended to achieve better quality of syphilis serology...
  22. pmc Borrelia burgdorferi complement regulator-acquiring surface protein 2 (CspZ) as a serological marker of human Lyme disease
    Peter Kraiczy
    Institute of Medical Microbiology and Infection Control, University Hospital of Frankfurt, Frankfurt am Main, Germany
    Clin Vaccine Immunol 15:484-91. 2008
    ..burgdorferi type strain B31 BbCRASP-2, consistent with the high percentage of serologically positive patients. These data indicate that BbCRASP-2 may be valuable for use in a widely effective serological assay...
  23. ncbi request reprint In vitro activities of faropenem, ertapenem, imipenem and meropenem against Borrelia burgdorferi s.l
    Rebecca Rödel
    Institute of Medical Microbiology and Infection Control, University Hospital of Frankfurt, Paul Ehrlich Str 40, D 60596 Frankfurt Main, Germany
    Int J Antimicrob Agents 30:83-6. 2007
    ..9%) reduction of the inoculum after 96 h of exposure to either drug at a concentration of three log2 unit dilutions above the respective MIC...
  24. ncbi request reprint Comparison of in vitro activities of tigecycline, doxycycline, and tetracycline against the spirochete Borrelia burgdorferi
    Louis Ates
    Institute of Medical Microbiology and Infection Control, University Hospital of Frankfurt, Frankfurt Main, Germany
    Ticks Tick Borne Dis 1:30-4. 2010
    ..Moreover, tigecycline was bactericidal at a concentration of 0.25 mg/L showing a >3 log(10) unit reduction of the initial inoculum, whereas for ceftriaxone a concentration of 2 mg/L was needed...
  25. pmc In vitro susceptibility of Borrelia spielmanii to antimicrobial agents commonly used for treatment of Lyme disease
    Kristina Morgenstern
    Institute of Medical Microbiology and Infection Control, University Hospital of Frankfurt, Frankfurt, Main, Germany
    Antimicrob Agents Chemother 53:1281-4. 2009
    ..spielmanii mainly parallels that of the other known human-pathogenic members of the B. burgdorferi sensu lato complex...
  26. pmc Thymidine-dependent Staphylococcus aureus small-colony variants: human pathogens that are relevant not only in cases of cystic fibrosis lung disease
    Silke Besier
    Institute of Medical Microbiology and Infection Control, Hospital of Johann Wolfgang Goethe University, Frankfurt Main, Paul Ehrlich Strasse 40, 60596 Frankfurt Main, Germany
    J Clin Microbiol 46:3829-32. 2008
    ..Furthermore, we provide evidence that the essential growth factor for TD-SCVs, i.e., thymidine, and its metabolite dTMP are present in various human specimens...
  27. ncbi request reprint [Flap Amputation in Mycobacterium chelonae Keratitis after Laser-in-situ Keratomileusis (LASIK)]
    Thomas Kohnen
    Klinik fur Augenheilkunde, Johann Wolfgang Goethe Universitat Frankfurt, Frankfurt am Main
    Klin Monbl Augenheilkd 220:634-7. 2003
    ..In the following report, we present clinical findings, microbiological investigation, treatment and outcome of the first case of Mycobacterium chelonae reported in Europe...
  28. ncbi request reprint Real-time PCR-based identification of bacterial and fungal pathogens from blood samples
    Madeleine Mai
    Institute of Laboratory Medicine, Microbiology and Infection Control, Northwest Medical Centre, Frankfurt Main, Germany
    Methods Mol Biol 1237:139-47. 2015
    ....
  29. pmc Serologic evidence for effective production of cytolysin A in Salmonella enterica serovars typhi and paratyphi A during human infection
    Christine von Rhein
    Institut für Medizinische Mikrobiologie und Krankenhaushygiene, Klinikum der Johann Wolfgang Goethe Universität Frankfurt am Main, Paul Ehrlich Str 40, 60596 Frankfurt am Main, Germany
    Infect Immun 74:6505-8. 2006
    ..We show here that human infections by these pathogens cause a specific antibody response to ClyA, indicating effective toxin production during infection...
  30. ncbi request reprint [Babesiosis in an immunocompromised German patient]
    Katja Häselbarth
    II Medizinische Klinik, Hegau Klinikum Singen, Virchowstrasse 10, Singen
    Med Klin (Munich) 103:104-7. 2008
    ..The disease manifestations show a broad clinical spectrum including a malaria-like syndrome. Fulminant and life-threatening infections have been described in the setting of asplenia and/or immunosuppression...
  31. ncbi request reprint First case of human babesiosis in Germany - Clinical presentation and molecular characterisation of the pathogen
    Katja Häselbarth
    Department of Internal Medicine II, Hegau Bodensee Medical Center, Singen, Germany
    Int J Med Microbiol 297:197-204. 2007
    ..Our findings suggest that human babesiosis occurs in Germany and can take a chronic course in immunocompromised individuals...
  32. ncbi request reprint A multiplex real-time PCR assay for rapid detection and differentiation of 25 bacterial and fungal pathogens from whole blood samples
    Lutz Eric Lehmann
    Klinik und Poliklinik für Anästhesie und Operative Intensivmedizin, Universitatsklinikum Bonn, Sigmund Freud Strasse 25, Bonn, Germany
    Med Microbiol Immunol 197:313-24. 2008
    ..8%. The analytical specificity in 102 healthy blood donors was 100%. Although further evaluation is warranted, our assay holds promise for more rapid pathogen identification in clinical sepsis...
  33. ncbi request reprint Systematic analysis highlights the key role of TLR2/NF-kappaB/MAP kinase signaling for IL-8 induction by macrophage-like THP-1 cells under influence of Borrelia burgdorferi lysates
    Christian D Sadik
    Pharmazentrum frankfurt ZAFES, Klinikum der Johann Wolfgang Goethe Universität Frankfurt am Main, 60590 Frankfurt am Main, Germany
    Int J Biochem Cell Biol 40:2508-21. 2008
    ..burgdorferi and beyond that suggest inhibition of IkappaB kinase and MEK1/2 kinases as promising pharmacological strategies aiming at IL-8 in early Lyme arthritis...