S Mergler

Summary

Affiliation: Humboldt University
Country: Germany

Publications

  1. ncbi R-type Ca(2+)-channel activity is associated with chromogranin A secretion in human neuroendocrine tumor BON cells
    S Mergler
    Universitätsklinikum Charité der Humboldt Universität zu Berlin, Medizinische Klinik m S Hepatologie und Gastroenterologie, Campus Virchow Klinikum, Augustenburger Platz 1, 13353 Berlin, Germany
    J Membr Biol 194:177-86. 2003
  2. doi Functional significance of thermosensitive transient receptor potential melastatin channel 8 (TRPM8) expression in immortalized human corneal endothelial cells
    Stefan Mergler
    Charite Universitatsmedizin Berlin, Campus Virchow Clinic, Department of Ophthalmology, Augustenburger Platz 1, 13353 Berlin, Germany Electronic address
    Exp Eye Res 116:337-49. 2013
  3. doi Calcium regulation by temperature-sensitive transient receptor potential channels in human uveal melanoma cells
    Stefan Mergler
    Charite Universitatsmedizin Berlin, Campus Virchow Clinic, Department of Ophthalmology, Augustenburger Platz 1, 13353 Berlin, Germany Electronic address
    Cell Signal 26:56-69. 2014
  4. doi Calcium regulation by thermo- and osmosensing transient receptor potential vanilloid channels (TRPVs) in human conjunctival epithelial cells
    Stefan Mergler
    Department of Ophthalmology, Campus Virchow Clinic, Charite, Universitatsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany
    Histochem Cell Biol 137:743-61. 2012
  5. doi Altered calcium regulation by thermosensitive transient receptor potential channels in etoposide-resistant WERI-Rb1 retinoblastoma cells
    Stefan Mergler
    Charite Universitatsmedizin Berlin, Campus Virchow Clinic, Department of Ophthalmology, Augustenburger Platz 1, 13353 Berlin, Germany
    Exp Eye Res 94:157-73. 2012
  6. doi Characterization of transient receptor potential vanilloid channel 4 (TRPV4) in human corneal endothelial cells
    Stefan Mergler
    Charite Universitatsmedizin Berlin, Campus Virchow Clinic, Department of Ophthalmology, Augustenburger Platz 1, 13353 Berlin, Germany
    Exp Eye Res 93:710-9. 2011
  7. doi TRPV channels mediate temperature-sensing in human corneal endothelial cells
    Stefan Mergler
    Charite Universitatsmedizin Berlin, Campus Virchow Clinic, Department of Ophthalmology, Augustenburger Platz 1, 13353 Berlin, Germany
    Exp Eye Res 90:758-70. 2010
  8. ncbi Transient receptor potential channel TRPM8 agonists stimulate calcium influx and neurotensin secretion in neuroendocrine tumor cells
    Stefan Mergler
    Department of Internal Medicine, Division of Hepatology and Gastroenterology, Charite Universitatsmedizin Berlin, Campus Virchow Klinikum, Berlin, Germany
    Neuroendocrinology 85:81-92. 2007
  9. ncbi The human corneal endothelium: new insights into electrophysiology and ion channels
    Stefan Mergler
    Department of Ophthalmology, Charite University Medicine Berlin, Campus Virchow Clinic, Augustenburger Platz 1, 13353 Berlin, Germany
    Prog Retin Eye Res 26:359-78. 2007
  10. doi Thermo-sensitive transient receptor potential vanilloid channel-1 regulates intracellular calcium and triggers chromogranin A secretion in pancreatic neuroendocrine BON-1 tumor cells
    Stefan Mergler
    Charite, Universitatsmedizin Berlin, Campus Virchow Clinic, Department of Ophthalmology, Berlin, Germany
    Cell Signal 24:233-46. 2012

Collaborators

Detail Information

Publications31

  1. ncbi R-type Ca(2+)-channel activity is associated with chromogranin A secretion in human neuroendocrine tumor BON cells
    S Mergler
    Universitätsklinikum Charité der Humboldt Universität zu Berlin, Medizinische Klinik m S Hepatologie und Gastroenterologie, Campus Virchow Klinikum, Augustenburger Platz 1, 13353 Berlin, Germany
    J Membr Biol 194:177-86. 2003
    ..Thus, R-type channels could play an important role in certain clinical characteristics of NETs, such as the hypersecretion syndrome...
  2. doi Functional significance of thermosensitive transient receptor potential melastatin channel 8 (TRPM8) expression in immortalized human corneal endothelial cells
    Stefan Mergler
    Charite Universitatsmedizin Berlin, Campus Virchow Clinic, Department of Ophthalmology, Augustenburger Platz 1, 13353 Berlin, Germany Electronic address
    Exp Eye Res 116:337-49. 2013
    ..Taken together, functional TRPM8 expression in HCEC-12 and freshly dissociated HCEC suggests that HCE function can adapt to thermal variations through activation of this channel subtype. ..
  3. doi Calcium regulation by temperature-sensitive transient receptor potential channels in human uveal melanoma cells
    Stefan Mergler
    Charite Universitatsmedizin Berlin, Campus Virchow Clinic, Department of Ophthalmology, Augustenburger Platz 1, 13353 Berlin, Germany Electronic address
    Cell Signal 26:56-69. 2014
    ..Identification of functional TRPV1, TRPM8, TRPA1 and CB1 expression in these tissues may provide novel drug targets for treatment of this aggressive neoplastic disease. ..
  4. doi Calcium regulation by thermo- and osmosensing transient receptor potential vanilloid channels (TRPVs) in human conjunctival epithelial cells
    Stefan Mergler
    Department of Ophthalmology, Campus Virchow Clinic, Charite, Universitatsmedizin Berlin, Augustenburger Platz 1, 13353, Berlin, Germany
    Histochem Cell Biol 137:743-61. 2012
    ..In conclusion, conjunctiva express TRPV1, TRPV2, and TRPV4 channels which may provide novel drug targets for dry eye therapeutics. Their usage may have fewer side effects than those currently encountered with less selective drugs...
  5. doi Altered calcium regulation by thermosensitive transient receptor potential channels in etoposide-resistant WERI-Rb1 retinoblastoma cells
    Stefan Mergler
    Charite Universitatsmedizin Berlin, Campus Virchow Clinic, Department of Ophthalmology, Augustenburger Platz 1, 13353 Berlin, Germany
    Exp Eye Res 94:157-73. 2012
    ..Therefore, using genetic approaches to upregulate TRPA1 expression could provide a means to induce etoposide sensitivity and suppress RB cell tumorigenesis...
  6. doi Characterization of transient receptor potential vanilloid channel 4 (TRPV4) in human corneal endothelial cells
    Stefan Mergler
    Charite Universitatsmedizin Berlin, Campus Virchow Clinic, Department of Ophthalmology, Augustenburger Platz 1, 13353 Berlin, Germany
    Exp Eye Res 93:710-9. 2011
    ..There is functional TRPV4 expression in HCEC-12 and in its clonal daughter cell lines based on Ca(2+) transients and underlying currents induced by known activators of this channel...
  7. doi TRPV channels mediate temperature-sensing in human corneal endothelial cells
    Stefan Mergler
    Charite Universitatsmedizin Berlin, Campus Virchow Clinic, Department of Ophthalmology, Augustenburger Platz 1, 13353 Berlin, Germany
    Exp Eye Res 90:758-70. 2010
    ..Taken together, TRPV activity modulation by temperature underlies essential homeostatic mechanisms contributing to the support of corneal endothelial function under different ambient conditions...
  8. ncbi Transient receptor potential channel TRPM8 agonists stimulate calcium influx and neurotensin secretion in neuroendocrine tumor cells
    Stefan Mergler
    Department of Internal Medicine, Division of Hepatology and Gastroenterology, Charite Universitatsmedizin Berlin, Campus Virchow Klinikum, Berlin, Germany
    Neuroendocrinology 85:81-92. 2007
    ..The regulation of NT secretion in NETs by TRPM8 may have a potential clinical implication in diagnosis or therapy...
  9. ncbi The human corneal endothelium: new insights into electrophysiology and ion channels
    Stefan Mergler
    Department of Ophthalmology, Charite University Medicine Berlin, Campus Virchow Clinic, Augustenburger Platz 1, 13353 Berlin, Germany
    Prog Retin Eye Res 26:359-78. 2007
    ..A better understanding of the (electro)physiological function of the cornea may lead to the development of clinical relevant new therapeutic and preventive measures...
  10. doi Thermo-sensitive transient receptor potential vanilloid channel-1 regulates intracellular calcium and triggers chromogranin A secretion in pancreatic neuroendocrine BON-1 tumor cells
    Stefan Mergler
    Charite, Universitatsmedizin Berlin, Campus Virchow Clinic, Department of Ophthalmology, Berlin, Germany
    Cell Signal 24:233-46. 2012
    ..Activation of TRPV1 translates into changes of intracellular Ca(2+), a known mechanism triggering the secretion of CgA. The clinical relevance of TRPV1 activation in NETs requires further investigations...
  11. pmc Thermosensitive transient receptor potential channels in human corneal epithelial cells
    Stefan Mergler
    Department of Ophthalmology, Campus Virchow Clinic, Charite Universitatsmedizin Berlin, Berlin, Germany
    J Cell Physiol 226:1828-42. 2011
    ..We conclude that HCEC possess in addition to thermosensitive TRPV3 activity TRPV1, TRPV2, and TRPV4 activity. Their activation confers temperature sensitivity at the ocular surface, which may protect the cornea against such stress...
  12. ncbi Insulin-like growth factor-1 increases intracellular calcium concentration in human primary neuroendocrine pancreatic tumor cells and a pancreatic neuroendocrine tumor cell line (BON-1) via R-type Ca2+ channels and regulates chromogranin a secretion in BO
    Stefan Mergler
    Medizinische Klinik mit Schwerpunkt Hepatologie und Gastroenterologie, Interdisziplinares Stoffwechsel Centrum Endokrinologie, Diabetes und Stoffwechsel, Charite Universitatsmedizin Berlin, Germany
    Neuroendocrinology 82:87-102. 2005
    ....
  13. ncbi EGF suppresses hydrogen peroxide induced Ca2+ influx by inhibiting L-type channel activity in cultured human corneal endothelial cells
    Stefan Mergler
    Medizinische Klinik m S Hepatologie und Gastroenterologie, Charite Universitatsmedizin Berlin, Campus Virchow Klinikum, Augustenburger Platz 1, D 13353 Berlin, Germany
    Exp Eye Res 80:285-93. 2005
    ..As EGF overcomes H2O2-induced rises in [Ca2+]i, its presence during eye bank storage could improve the outcome of corneal transplant surgery...
  14. ncbi Ca(2+) channel properties in neuroendocrine tumor cell cultures investigated by whole-cell patch-clamp technique
    Stefan Mergler
    Medizinische Klinik mit Schwerpunkt Hepatologie, Gastroenterologie, Endokrinologie und Stoffwechsel, Universitatsklinikum Charite, Campus Virchow Klinikum, Humboldt Universitat zu Berlin, Berlin, Germany
    Ann N Y Acad Sci 1014:137-9. 2004
    ..Therefore, these data on the characteristics of VOCCs could be useful in the development of a novel therapy for NET disease; for example, specific VOCC subtype modulators may help...
  15. ncbi Ca2+ channel characteristics in neuroendocrine tumor cell cultures analyzed by color contour plots
    Stefan Mergler
    Universitätsklinikum Charité der Humboldt Universität zu Berlin, Medizinische Klinik m S Hepatologie und Gastroenterologie, Campus Virchow Klinikum, Augustenburger Platz 1, 13353 Berlin, Germany
    J Neurosci Methods 129:169-81. 2003
    ....
  16. ncbi Calcium influx induced by activation of receptor tyrosine kinases in SV40-transfected human corneal endothelial cells
    Stefan Mergler
    Medizinische Klinik m S Hepatologie und Gastroenterologie, Universitätsklinikum Charité der Humboldt Universität zu Berlin, Campus Virchow Klinikum, Augustenburger Platz 1, D 13353 Berlin, Germany
    Exp Eye Res 77:485-95. 2003
    ..These data regarding Ca(2+) inflow through Ca(2+) channels could be useful for investigation of culture and vitality conditions of HCEC...
  17. doi [Physiology of the human corneal endothelium--new insights from electrophysiological investigations]
    S Mergler
    Klinik fur Augenheilkunde, Charite Universitatsmedizin Berlin, Augustenburger Platz 1, Berlin
    Klin Monbl Augenheilkd 228:520-4. 2011
    ....
  18. doi Capsaicin induces cytotoxicity in pancreatic neuroendocrine tumor cells via mitochondrial action
    M Skrzypski
    Department of Hepatology and Gastroenterology and the Interdisciplinary Centre of Metabolism Endocrinology, Diabetes and Metabolism, Charite University Medicine Berlin, 13353 Berlin, Germany Department of Animal Physiology and Biochemistry, Poznan University of Life Sciences, 60 637 Poznan, Poland Electronic address
    Cell Signal 26:41-8. 2014
    ..Stimulation of ROS generation by CAP appears to be a secondary effect, not related to CAP-induced cytotoxicity. These results justify further evaluation of CAP in modulating pancreatic NETs in vivo. ..
  19. doi TRPV6 channel modulates proliferation of insulin secreting INS-1E beta cell line
    M Skrzypski
    Department of Hepatology and Gastroenterology and the Interdisciplinary Centre of Metabolism Endocrinology, Diabetes and Metabolism, Charite University Medicine Berlin, 13353 Berlin, Germany Department of Animal Physiology and Biochemistry, Poznan University of Life Sciences, 60 637Poznań, Poland
    Biochim Biophys Acta 1853:3202-10. 2015
    ..In contrast, TRPV6 fails to influence insulin secretion...
  20. doi Activation of TRPV4 channel in pancreatic INS-1E beta cells enhances glucose-stimulated insulin secretion via calcium-dependent mechanisms
    M Skrzypski
    Department of Hepatology and Gastroenterology, The Interdisciplinary Centre of Metabolism Endocrinology, Diabetes and Metabolism, Charite University Medicine Berlin, 13353 Berlin, Germany Department of Animal Physiology and Biochemistry, Poznan University of Life Sciences, 60 637 Poznan, Poland
    FEBS Lett 587:3281-7. 2013
    ..In the presence of the TRPV channel blocker ruthenium red (RuR) or after suppression of TRPV4 protein production, TRPV4 activators failed to increase [Ca(2+)]i and insulin secretion in INS-1E cells. ..
  21. ncbi Activation of neuroendocrine L-type channels (alpha1D subunits) in retinal pigment epithelial cells and brain neurons by pp60(c-src)
    O Strauss
    Institut für Klinische Physiologie, Universitatsklinikum Benjamin Franklin, Freie Universitat Berlin, Hindenburgdamm 30, Berlin, 12200, Germany
    Biochem Biophys Res Commun 270:806-10. 2000
    ..Thus, L-type channels of the neuroendocrine subtype can be expressed in epithelial cells and are activated by tyrosine kinase of the src subtype. This kind of regulation is also suggested for brain-derived neurons...
  22. ncbi Gastrin transactivates the chromogranin A gene through MEK-1/ERK- and PKC-dependent phosphorylation of Sp1 and CREB
    Thorsten Cramer
    Laboratory for Angiogenesis and Tumor Metastasis, Charite Universitatsmedizin Berlin, Campus Virchow Klinikum, Germany
    Cell Signal 20:60-72. 2008
    ....
  23. ncbi Stimulation of L-type Ca(2+) channels by increase of intracellular InsP3 in rat retinal pigment epithelial cells
    Stefan Mergler
    Universitätsklinikum Charité der Humboldt Universität zu Berlin, Medizinische Klinik, Hepatologie und Gastroenterologie, Campus Virchow Klinikum, Augustenburger Platz 1, Berlin, 13353, Germany
    Exp Eye Res 74:29-40. 2002
    ..This is enabled by depletion of cytosolic Ca(2+)stores and tyrosine kinase-dependent activation of L-type channels...
  24. doi Insulinostatic activity of cerebellin--evidence from in vivo and in vitro studies in rats
    Mathias Z Strowski
    Medizinische Klinik mit Schwerpunkt Hepatologie, Gastroenterologie and Interdisziplinäres Stoffwechsel Centrum Endokrinologie, Diabetes und Stoffwechsel Charité Universitätsmedizin Berlin, Campus Virchow Klinikum, Augustenburger Platz 1, 13353 Berlin, Germany
    Regul Pept 157:19-24. 2009
    ..Furthermore, we identify CER as an insulinostatic factor, which decreases intracellular cAMP production and calcium in INS-1 cells...
  25. doi Characterization of voltage operated R-type Ca2+ channels in modulating somatostatin receptor subtype 2- and 3-dependent inhibition of insulin secretion from INS-1 cells
    Stefan Mergler
    Augenklinik, Charite Universitatsmedizin Berlin, Campus Virchow Klinikum, 13353 Berlin, Germany
    Cell Signal 20:2286-95. 2008
    ..In contrast, the R-type calcium channel does not mediate the effects of SST3-ag. on insulin secretion. We conclude that R-type channels play a major role in the inhibition of insulin secretion by somatostatin in INS-1 cells...
  26. ncbi Effects of spironolactone on corneal allograft survival in the rat
    Ljiljana Otasevic
    Department of Ophthalmology, Charite University Medicine Berlin, Berlin, Germany
    Ophthalmic Res 39:325-9. 2007
    ..In this study, we investigated the effects of spironolactone on the prevention of corneal allograft rejection in a MHC class I/II mismatch rat corneal transplant model...
  27. ncbi Gene transfer of cyto-protective molecules in corneal endothelial cells and cultured corneas: analysis of protective effects in vitro and in vivo
    Nianqiao Gong
    Department of Ophthalmology, Charite University Medicine Berlin, Germany
    Biochem Biophys Res Commun 357:302-7. 2007
    ..However, gene transfer of a single cyto-protective molecule does not prevent its rejection upon transplantation in a MHC class I/II disparate rat model...
  28. ncbi Transient receptor potential vanilloid 1 activation induces inflammatory cytokine release in corneal epithelium through MAPK signaling
    Fan Zhang
    Biological Sciences, The State University of New York, College of Optometry, New York, New York 10036, USA
    J Cell Physiol 213:730-9. 2007
    ..Taken together, TRPV1 receptors may play a role in mediating corneal epithelial inflammatory mediator secretion and subsequent hyperalgesia...
  29. ncbi PKC isoform-specific enhancement of capacitative calcium entry in human corneal epithelial cells
    Fan Zhang
    Department of Biological Sciences, College of Optometry, State University of New York, New York, New York 10036, USA
    Invest Ophthalmol Vis Sci 47:3989-4000. 2006
    ..To determine in human corneal epithelial cells (HCECs) the role of protein kinase C (PKC) in mediating epidermal growth factor (EGF)-induced stimulation of store-operated channel (SOC) activity and capacitative calcium entry (CCE)...
  30. pmc TRPC4 knockdown suppresses epidermal growth factor-induced store-operated channel activation and growth in human corneal epithelial cells
    Hua Yang
    College of Optometry, State University of New York, New York 10036, USA
    J Biol Chem 280:32230-7. 2005
    ..Thus, TRPC4 is a component of SOC in human corneal epithelial cells whose activation by EGF is requisite for an optimum mitogenic response to this growth factor...
  31. ncbi Valine-286 residue in the third intracellular loop of the cholecystokinin 2 receptor exerts a pivotal role in cholecystokinin 2 receptor mediated intracellular signal transduction in human colon cancer cells
    Hong Gang Yu
    Laboratory for Experimental Gastroenterology, Department of Medicine I, St Josef Hospital, Ruhr University of Bochum, Gudrunstr 56, D 44791 Bochum
    Cell Signal 17:1505-15. 2005
    ..The valine-287 residue in third intracellular loop of CCK2R plays a pivotal role in CCK2R mediated intracellular signal transduction...