Karl Heinz Klempnauer

Summary

Country: Germany

Publications

  1. pmc Oncogenic point mutations in the Myb DNA-binding domain alter the DNA-binding properties of Myb at a physiological target gene
    Olga Ivanova
    Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, Wilhelm Klemm Strasse 2, D 48149 Munster, Germany
    Nucleic Acids Res 35:7237-47. 2007
  2. pmc Tumor suppressor protein Pdcd4 interacts with Daxx and modulates the stability of Daxx and the Hipk2-dependent phosphorylation of p53 at serine 46
    N Kumar
    1 Institut für Biochemie, Westfalische Wilhelms Universitat Munster, Munster, Germany 2 Graduate School of Chemistry GSC MS, Westfalische Wilhelms Universitat Munster, Germany
    Oncogenesis 2:e37. 2013
  3. pmc B-Myb switches from Cyclin/Cdk-dependent to Jnk- and p38 kinase-dependent phosphorylation and associates with SC35 bodies after UV stress
    E Werwein
    Institut fur Biochemie, Wilhelm Klemm Straße 2, D 48149 Munster, Germany
    Cell Death Dis 4:e511. 2013
  4. ncbi request reprint Regulation of the cyclin D1 and cyclin A1 promoters by B-Myb is mediated by Sp1 binding sites
    Thorsten Bartusel
    Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, Germany
    Gene 351:171-80. 2005
  5. pmc v-Myb mediates cooperation of a cell-specific enhancer with the mim-1 promoter
    Olesya Chayka
    Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, Wilhelm Klemm Strasse 2, D 48149 Munster, Germany
    Mol Cell Biol 25:499-511. 2005
  6. ncbi request reprint A dual activation mechanism for Myb-responsive genes in myelomonocytic cells
    Vichanan Yamkamon
    Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, Wilhelm Klemm Str 2, D 48149 Munster, Germany
    Blood Cells Mol Dis 40:219-26. 2008
  7. ncbi request reprint Identification of a Myb-responsive enhancer of the chicken C/EBPbeta gene
    Jörg Kintscher
    Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, Wilhelm Klemm Str 2, D 48149 Munster, Germany
    Oncogene 23:5807-14. 2004
  8. pmc Myb-induced chromatin remodeling at a dual enhancer/promoter element involves non-coding rna transcription and is disrupted by oncogenic mutations of v-myb
    Carola Wilczek
    Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, D 48149 Munster, Germany
    J Biol Chem 284:35314-24. 2009
  9. ncbi request reprint Analysis of DNase I-hypersensitive sites in the chromatin of the chicken C/EBPbeta gene reveals multiple cis-regulatory elements
    Jörg Kintscher
    Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, D 48149 Munster, Germany
    DNA Cell Biol 22:201-8. 2003
  10. ncbi request reprint The transformation suppressor protein Pdcd4 shuttles between nucleus and cytoplasm and binds RNA
    Maret Böhm
    Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, Wilhelm Klemm Str 2, D 48149 Münster1, Germany
    Oncogene 22:4905-10. 2003

Collaborators

  • Holger Gundelach
  • Thomas J Schmidt
  • Constanze Bonifer
  • N Kumar
  • Reiner Peters
  • Detlef Lang
  • Daniel Braas
  • Johanna Ohnheiser
  • Olesya Chayka
  • Lena Wedeken
  • Olesja Fehler
  • Thorsten Bartusel
  • Eugen Werwein
  • Jörg Kintscher
  • Nils Wethkamp
  • Carola Wilczek
  • Annette Plachetka
  • Olga Ivanova
  • Vichanan Yamkamon
  • Jan P Müller
  • Astrid Haas
  • E Werwein
  • Caroline Schomburg
  • Simone Steinmann
  • Priyanka Singh
  • Stephan Schubert
  • Maret Böhm
  • Hartmut Appl
  • Dana Kattmann
  • Dörthe Ahlbory
  • Nadja Bitomsky
  • Claudia Schwartz
  • Josef Miethe
  • Abhiruchi Biyanee
  • Eva Ferlemann
  • Diana Ulrich
  • Fernando B da Costa
  • Wolfgang Schuehly
  • M Dzuganova
  • Anke Jakobs
  • C Usadel
  • Anna Coulibaly
  • Thore Schmedt
  • Clemens Usadel
  • Nora Obermann
  • Heiko Hoffmann
  • Jeffrey D Singer
  • Benjamin Hirschi
  • Svitlana Melnik
  • Pimpicha Patmasiriwat
  • Sebastian Horstmann
  • Jan Peter Siebrasse
  • Sigrun Mink
  • Kristina Beck
  • Bastian Budde
  • Mirco Schmolke
  • Anne Brehmer-Fastnacht
  • Dorit Wenning
  • Kirsty Sawicka

Detail Information

Publications29

  1. pmc Oncogenic point mutations in the Myb DNA-binding domain alter the DNA-binding properties of Myb at a physiological target gene
    Olga Ivanova
    Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, Wilhelm Klemm Strasse 2, D 48149 Munster, Germany
    Nucleic Acids Res 35:7237-47. 2007
    ..Our data demonstrate for the first time that oncogenic activation of Myb alters its DNA-binding specificity at a physiological Myb target gene...
  2. pmc Tumor suppressor protein Pdcd4 interacts with Daxx and modulates the stability of Daxx and the Hipk2-dependent phosphorylation of p53 at serine 46
    N Kumar
    1 Institut für Biochemie, Westfalische Wilhelms Universitat Munster, Munster, Germany 2 Graduate School of Chemistry GSC MS, Westfalische Wilhelms Universitat Munster, Germany
    Oncogenesis 2:e37. 2013
    ..Overall, our data demonstrate that Pdcd4 is a novel modulator of Daxx function and provide evidence for a role of Pdcd4 in restraining p53 activity in unstressed cells...
  3. pmc B-Myb switches from Cyclin/Cdk-dependent to Jnk- and p38 kinase-dependent phosphorylation and associates with SC35 bodies after UV stress
    E Werwein
    Institut fur Biochemie, Wilhelm Klemm Straße 2, D 48149 Munster, Germany
    Cell Death Dis 4:e511. 2013
    ..Taken together, we have identified Jnk and p38 kinase as novel regulators of B-Myb and established the localization of phosphorylated B-Myb in SC35 speckles as a potential novel regulatory mechanism for B-Myb in UV irradiated cells...
  4. ncbi request reprint Regulation of the cyclin D1 and cyclin A1 promoters by B-Myb is mediated by Sp1 binding sites
    Thorsten Bartusel
    Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, Germany
    Gene 351:171-80. 2005
    ....
  5. pmc v-Myb mediates cooperation of a cell-specific enhancer with the mim-1 promoter
    Olesya Chayka
    Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, Wilhelm Klemm Strasse 2, D 48149 Munster, Germany
    Mol Cell Biol 25:499-511. 2005
    ....
  6. ncbi request reprint A dual activation mechanism for Myb-responsive genes in myelomonocytic cells
    Vichanan Yamkamon
    Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, Wilhelm Klemm Str 2, D 48149 Munster, Germany
    Blood Cells Mol Dis 40:219-26. 2008
    ..Our results show that C/EBPalpha expression is down-regulated by v-Myb. Thus, v-Myb affects the expression of two C/EBP family members in opposite directions...
  7. ncbi request reprint Identification of a Myb-responsive enhancer of the chicken C/EBPbeta gene
    Jörg Kintscher
    Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, Wilhelm Klemm Str 2, D 48149 Munster, Germany
    Oncogene 23:5807-14. 2004
    ..Apart from the identification of a novel Myb-dependent enhancer, our work demonstrates the potential of chromatin structure analysis for the identification of Myb target sites...
  8. pmc Myb-induced chromatin remodeling at a dual enhancer/promoter element involves non-coding rna transcription and is disrupted by oncogenic mutations of v-myb
    Carola Wilczek
    Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, D 48149 Munster, Germany
    J Biol Chem 284:35314-24. 2009
    ....
  9. ncbi request reprint Analysis of DNase I-hypersensitive sites in the chromatin of the chicken C/EBPbeta gene reveals multiple cis-regulatory elements
    Jörg Kintscher
    Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, D 48149 Munster, Germany
    DNA Cell Biol 22:201-8. 2003
    ..Taken together, our data suggest that the expression of the C/EBPbeta gene in myelomonocytic cells is controlled by multiple cell-type specific cis-acting sequences located both upstream and downstream of the gene...
  10. ncbi request reprint The transformation suppressor protein Pdcd4 shuttles between nucleus and cytoplasm and binds RNA
    Maret Böhm
    Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, Wilhelm Klemm Str 2, D 48149 Münster1, Germany
    Oncogene 22:4905-10. 2003
    ..Taken together, our data raise the possibility that Pdcd4 is involved in some aspect of nuclear RNA metabolism in addition to its suspected role in protein translation...
  11. ncbi request reprint Targeted disruption of c-myb in the chicken pre B-cell line DT40
    Hartmut Appl
    Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, Wilhelm Klemm Str 2, D 48143 Munster, Germany
    Oncogene 21:3076-81. 2002
    ..The c-myb knock-out cells described here should provide a useful model system for the identification and characterization of c-myb target genes in B-lymphoid cells...
  12. pmc Daxx is a transcriptional repressor of CCAAT/enhancer-binding protein beta
    Nils Wethkamp
    Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, Wilhelm Klemm Strasse 2, Munster D 48149, Germany
    J Biol Chem 284:28783-94. 2009
    ..Overall, our data identify Daxx as a new negative regulator of C/EBPbeta and provide first clues for a link between abrogation of Daxx-C/EBPbeta complex formation and APL remission...
  13. pmc B-Myb promotes S-phase independently of its sequence-specific DNA binding activity and interacts with polymerase delta-interacting protein 1 (Pdip1)
    Eugen Werwein
    Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, Munster, Germany
    Cell Cycle 11:4047-58. 2012
    ..Taken together, our work suggests a novel role for B-Myb in S-phase that appears to be independent of its sequence-specific DNA-binding activity and its ability to stimulate the expression of bona fide B-Myb target genes...
  14. pmc The glioma-amplified sequence 41 gene (GAS41) is a direct Myb target gene
    Daniel Braas
    Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, Wilhelm Klemm Str 2, D 48149 Munster, Germany
    Nucleic Acids Res 32:4750-7. 2004
    ..We have also analysed the GAS41 replication origin in myelomonocytic cells and have failed to observe significant differences in origin activity in cells expressing or not expressing v-Myb...
  15. pmc The promoter regions of the Myb-regulated Adora2B and Mcm4 genes co-localize with origins of DNA replication
    Holger Gundelach
    Institute for Biochemistry, Westfalische Wilhelms Universitat Munster, Wilhelm Klemm Str, 2, D 48149 Munster, Germany
    BMC Mol Biol 8:75. 2007
    ..Because of this finding we have asked whether other Myb-regulated genes are also associated with DNA replication origins...
  16. pmc Recruitment of p300 by C/EBPbeta triggers phosphorylation of p300 and modulates coactivator activity
    Claudia Schwartz
    Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, Wilhelm Klemm Strasse 2, D 48149 Munster, Germany
    EMBO J 22:882-92. 2003
    ....
  17. doi request reprint Programmed cell death 4 protein (Pdcd4) and homeodomain-interacting protein kinase 2 (Hipk2) antagonistically control translation of Hipk2 mRNA
    Johanna Ohnheiser
    Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, Wilhelm Klemm Str 2, D 48149 Munster, Germany
    Biochim Biophys Acta 1853:1564-73. 2015
    ..Furthermore, the effect of Hipk2 on the translation of Hipk1 RNA suggests that Hipk2 and Pdcd4 can act in similar manner to control the translation of other mRNAs. ..
  18. ncbi request reprint The cooperation of B-Myb with the coactivator p300 is orchestrated by cyclins A and D1
    Stephan Schubert
    Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, Wilhelm Klemm Str 2, D 48149 Munster, Germany
    Oncogene 23:1392-404. 2004
    ..Taken together they suggest that the coactivator p300 plays an important role in this regulation and that the cooperation of B-Myb and p300 is orchestrated by cyclins A and D1...
  19. ncbi request reprint Transactivation mediated by B-Myb is dependent on TAF(II)250
    Thorsten Bartusel
    Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, Wilhelm Klemm Str 2, D 48149 Munster, Germany
    Oncogene 22:2932-41. 2003
    ..The finding that B-Myb transactivation is dependent on a key coactivator involved in cell cycle control is consistent with and strengthens the idea that B-Myb plays a crucial role as a transcription factor in proliferating cells...
  20. ncbi request reprint Identification and characterization of the Myb-inducible promoter of the chicken adenosine receptor 2B gene
    Dana Kattmann
    Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, Wilhelm Klemm Str 2, D 48149 Munster, Germany
    Oncogene 21:4663-72. 2002
    ..Taken together, our data suggest that the activation of the A2B-AR promoter by Myb depends on the simultaneous binding of a large number of Myb molecules...
  21. ncbi request reprint Disruption of B-myb in DT40 cells reveals novel function for B-Myb in the response to DNA-damage
    Dörthe Ahlbory
    Institute for Biochemistry, Westfalische Wilhelms Universitat Munster, Wilhelm Klemm Str 2, Munster D 48149, Germany
    Oncogene 24:7127-34. 2005
    ..Our work provides the first direct evidence for a novel function of B-Myb in the response to DNA-damage. The cells described here should be a useful model to characterize this function in more detail...
  22. pmc C/EBPbeta induces chromatin opening at a cell-type-specific enhancer
    Annette Plachetka
    Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, Wilhelm Klemm Str 2, D 48149 Munster, Germany
    Mol Cell Biol 28:2102-12. 2008
    ..Our work identifies a novel function for C/EBPbeta in the initial steps of a localized chromatin opening at a specific, physiologically relevant target region...
  23. pmc Interaction and cooperation of the CCAAT-box enhancer-binding protein β (C/EBPβ) with the homeodomain-interacting protein kinase 2 (Hipk2)
    Simone Steinmann
    Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, Wilhelm Klemm Strasse 2, D 48149 Munster, Germany
    J Biol Chem 288:22257-69. 2013
    ..Overall, our data identify Hipk2 as a novel regulator of C/EBPβ and implicate different protein kinases in the cooperation of p300 with C/EBPβ and C/EBPα. ..
  24. pmc Tumor suppressor protein Pdcd4 inhibits translation of p53 mRNA
    Lena Wedeken
    Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, D 48149 Munster, Germany
    J Biol Chem 286:42855-62. 2011
    ..Overall, our work demonstrates for the first time that Pdcd4 is directly involved in translational suppression of a natural mRNA with a 5'-structured UTR and provides novel insight into the translational control of p53 expression...
  25. pmc An evolutionarily conserved interaction of tumor suppressor protein Pdcd4 with the poly(A)-binding protein contributes to translation suppression by Pdcd4
    Olesja Fehler
    Institute for Biochemistry, Westfalische Wilhelms Universitat Munster, Wilhelm Klemm Str 2, D 48149 Munster, Germany
    Nucleic Acids Res 42:11107-18. 2014
    ..Overall, our work identifies PABP as a novel functionally relevant Pdcd4 interaction partner that contributes to the regulation of translation by Pdcd4. ..
  26. doi request reprint Natural sesquiterpene lactones as inhibitors of Myb-dependent gene expression: structure-activity relationships
    Caroline Schomburg
    Institute of Pharmaceutical Biology and Phytochemistry IPBP, University of Munster, Hittorfstr 56, D 48149 Munster, Germany
    Eur J Med Chem 63:313-20. 2013
    ....
  27. ncbi request reprint Transformation suppressor protein Pdcd4 interferes with JNK-mediated phosphorylation of c-Jun and recruitment of the coactivator p300 by c-Jun
    Nadja Bitomsky
    Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, Wilhelm Klemm Str 2, D 48149, Germany
    Oncogene 23:7484-93. 2004
    ..In addition to affecting c-Jun phosphorylation, Pdcd4 blocks the recruitment of the coactivator p300 by c-Jun. Taken together, our results strongly suggest that Pdcd4 is directly involved in the regulation of c-Jun activity...
  28. ncbi request reprint Analysis of DNase I-hypersensitive sites in the chromatin of the chicken adenosine receptor 2B gene reveals multiple cell-type-specific cis-regulatory elements
    Daniel Braas
    Institut fur Biochemie, Westfalische Wilhelms Universitat Munster, Wilhelm Klemm Strasse 2, Germany
    Gene 303:157-64. 2003
    ..Taken together, our data suggest that the expression of the A2B adenosine receptor gene in myelomonocytic cells is controlled by multiple cell type-specific cis-acting sequences located upstream and within the intron of the gene...
  29. pmc Association of Tumor Suppressor Protein Pdcd4 With Ribosomes Is Mediated by Protein-Protein and Protein-RNA Interactions
    Lena Wedeken
    Institute for Biochemistry, Westfalische Wilhelms Universitat Munster, Munster, Germany
    Genes Cancer 1:293-301. 2010
    ..Their work provides the first direct evidence for an essential role of the Pdcd4 RNA binding activity in vivo and suggests that RNA binding is required for recruiting Pdcd4 to the translation machinery...