J Vanselow

Summary

Affiliation: Research Institute for the Biology of Farm Animals
Country: Germany

Publications

  1. doi request reprint Cultured bovine granulosa cells rapidly lose important features of their identity and functionality but partially recover under long-term culture conditions.
    Vengala Rao Yenuganti
    Institute of Reproductive Biology, Leibniz Institute for Farm Animal Biology FBN, Wilhelm Stahl Allee 2, 18196, Dummerstorf, Germany
    Cell Tissue Res 368:397-403. 2017
  2. pmc On the relevance of technical variation due to building pools in microarray experiments
    Henrik Rudolf
    Institut für Genetik und Biometrie, Leibniz Institut für Nutztierbiologie, Dummerstorf, DE, Germany
    BMC Genomics 16:1027. 2015
  3. doi request reprint Maternal high-protein diet during pregnancy, but not during suckling, induced altered expression of an increasing number of hepatic genes in adult mouse offspring
    Jens Vanselow
    Institute of Reproductive Biology, Leibniz Institute for Farm Animal Biology FBN, 18196, Dummerstorf, Germany
    Eur J Nutr 55:917-30. 2016
  4. pmc Suitable housekeeping genes for normalization of transcript abundance analysis by real-time RT-PCR in cultured bovine granulosa cells during hypoxia and differential cell plating density
    Vijay S Baddela
    Animal Biochemistry Division, National Dairy Research Institute, Karnal 132001, Haryana, India
    Reprod Biol Endocrinol 12:118. 2014
  5. pmc Research resource: preovulatory LH surge effects on follicular theca and granulosa transcriptomes
    Lane K Christenson
    Department of Molecular and Integrative Physiology, University of Kansas Medical Center, 3075 HLSIC, 3901 Rainbow Boulevard, Kansas City, Kansas 66160, USA
    Mol Endocrinol 27:1153-71. 2013
  6. pmc Upstream stimulating factors 1 and 2 enhance transcription from the placenta-specific promoter 1.1 of the bovine cyp19 gene
    Rainer Fürbass
    Research Unit Molecular Biology, Research Institute for the Biology of Farm Animals FBN, Wilhelm Stahl Allee 2, 18196 Dummerstorf, Germany
    BMC Mol Biol 11:5. 2010
  7. ncbi request reprint Placenta-specific transcripts of the aromatase encoding gene include different untranslated first exons in sheep and cattle
    J Vanselow
    Research Institute for the Biology of Farm Animals, Dummerstorf, Germany
    Eur J Biochem 265:318-24. 1999
  8. doi request reprint The bovine genome contains three differentially methylated paralogous copies of the P450c17 encoding gene (CYP17A1)
    Jens Vanselow
    Research Unit Molecular Biology, Leibniz Institute for Farm Animal Biology FBN, 18196 Dummerstorf, Germany
    Gen Comp Endocrinol 170:475-9. 2011
  9. ncbi request reprint Expression of the aromatase cytochrome P450 encoding gene in cattle and sheep
    J Vanselow
    Research Institute for the Biology of Farm Animals, Wilhelm Stahl Allee 2, 18196, Dummerstorf, Germany
    J Steroid Biochem Mol Biol 79:279-88. 2001
  10. doi request reprint DNA methylation is not involved in preovulatory down-regulation of CYP11A1, HSD3B1, and CYP19A1 in bovine follicles but may have a role in permanent silencing of CYP19A1 in large granulosa lutein cells
    Jens Vanselow
    Forschungsbereich Molekularbiologie, Forschungsinstitut fur die Biologie landwirtschaftlicher Nutztiere FBN, Dummerstorf, Germany
    Biol Reprod 82:289-98. 2010

Collaborators

Detail Information

Publications36

  1. doi request reprint Cultured bovine granulosa cells rapidly lose important features of their identity and functionality but partially recover under long-term culture conditions.
    Vengala Rao Yenuganti
    Institute of Reproductive Biology, Leibniz Institute for Farm Animal Biology FBN, Wilhelm Stahl Allee 2, 18196, Dummerstorf, Germany
    Cell Tissue Res 368:397-403. 2017
    ..We conclude that only long-term culture conditions (>4 days) allow the cells to recover from plating stress and to re-acquire characteristic granulosa cell features...
  2. pmc On the relevance of technical variation due to building pools in microarray experiments
    Henrik Rudolf
    Institut für Genetik und Biometrie, Leibniz Institut für Nutztierbiologie, Dummerstorf, DE, Germany
    BMC Genomics 16:1027. 2015
    ....
  3. doi request reprint Maternal high-protein diet during pregnancy, but not during suckling, induced altered expression of an increasing number of hepatic genes in adult mouse offspring
    Jens Vanselow
    Institute of Reproductive Biology, Leibniz Institute for Farm Animal Biology FBN, 18196, Dummerstorf, Germany
    Eur J Nutr 55:917-30. 2016
    ..In this study, we analyzed short-term and sustainable effects of a prenatal versus early postnatal maternal high-protein diet on growth and hepatic gene expression in mouse offspring...
  4. pmc Suitable housekeeping genes for normalization of transcript abundance analysis by real-time RT-PCR in cultured bovine granulosa cells during hypoxia and differential cell plating density
    Vijay S Baddela
    Animal Biochemistry Division, National Dairy Research Institute, Karnal 132001, Haryana, India
    Reprod Biol Endocrinol 12:118. 2014
    ..The current study identified two reliable housekeeping genes useful for gene normalization in granulosa cells under different in vitro conditions...
  5. pmc Research resource: preovulatory LH surge effects on follicular theca and granulosa transcriptomes
    Lane K Christenson
    Department of Molecular and Integrative Physiology, University of Kansas Medical Center, 3075 HLSIC, 3901 Rainbow Boulevard, Kansas City, Kansas 66160, USA
    Mol Endocrinol 27:1153-71. 2013
    ..This Research Resource provides an easy-to-access global evaluation of LH regulation in TCs and GCs that implicates numerous molecular pathways heretofore unknown within the follicle. ..
  6. pmc Upstream stimulating factors 1 and 2 enhance transcription from the placenta-specific promoter 1.1 of the bovine cyp19 gene
    Rainer Fürbass
    Research Unit Molecular Biology, Research Institute for the Biology of Farm Animals FBN, Wilhelm Stahl Allee 2, 18196 Dummerstorf, Germany
    BMC Mol Biol 11:5. 2010
    ..The aim of this work was to identify and characterise the placental E-box (-340)-binding protein(s) (E-BP) as a step towards understanding how the expression of Cyp19 is regulated in the bovine placenta...
  7. ncbi request reprint Placenta-specific transcripts of the aromatase encoding gene include different untranslated first exons in sheep and cattle
    J Vanselow
    Research Institute for the Biology of Farm Animals, Dummerstorf, Germany
    Eur J Biochem 265:318-24. 1999
    ....
  8. doi request reprint The bovine genome contains three differentially methylated paralogous copies of the P450c17 encoding gene (CYP17A1)
    Jens Vanselow
    Research Unit Molecular Biology, Leibniz Institute for Farm Animal Biology FBN, 18196 Dummerstorf, Germany
    Gen Comp Endocrinol 170:475-9. 2011
    ..From these data we conclude that the bovine genome contains three paralogous copies of the CYP17A1 gene, of which two (CYP17A1b and CYP17A1x) might be silenced by epigenetic modification (promoter methylation)...
  9. ncbi request reprint Expression of the aromatase cytochrome P450 encoding gene in cattle and sheep
    J Vanselow
    Research Institute for the Biology of Farm Animals, Wilhelm Stahl Allee 2, 18196, Dummerstorf, Germany
    J Steroid Biochem Mol Biol 79:279-88. 2001
    ..The co-localisation of an E-box element within one of the placenta-specific HS suggests that this element is important for Cyp19 expression in the bovine placenta...
  10. doi request reprint DNA methylation is not involved in preovulatory down-regulation of CYP11A1, HSD3B1, and CYP19A1 in bovine follicles but may have a role in permanent silencing of CYP19A1 in large granulosa lutein cells
    Jens Vanselow
    Forschungsbereich Molekularbiologie, Forschungsinstitut fur die Biologie landwirtschaftlicher Nutztiere FBN, Dummerstorf, Germany
    Biol Reprod 82:289-98. 2010
    ....
  11. pmc Expression profiling of a high-fertility mouse line by microarray analysis and qPCR
    Jens Vanselow
    Forschungsinstitut fur die Biologie landwirtschaftlicher Nutztiere FBN, Wilhelm Stahl Allee 2, 18196 Dummerstorf, Germany
    BMC Genomics 9:307. 2008
    ..Ovaries from 30 animals of each line were collected at the metestrous stage, combined to 6 pools each, and processed for microarray analysis...
  12. doi request reprint DNA methylation of placenta-specific Cyp19 promoters of cattle and sheep
    J Vanselow
    Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere, Wilhelm Stahl Allee 2, Dummerstorf, Germany
    Exp Clin Endocrinol Diabetes 116:437-42. 2008
    ....
  13. ncbi request reprint Cattle and sheep use different promoters to direct the expression of the aromatase cytochrome P450 encoding gene, Cyp19, during pregnancy
    Jens Vanselow
    Research Institute for the Biology of Farm Animals, Research Unit Molecular Biology, Wilhelm Stahl Allee 2, 18196 Dummerstorf, Germany
    Domest Anim Endocrinol 27:99-114. 2004
    ..1 derived transcripts. Contrary to the bovine, the sheep corpus luteum also showed considerable P1.5 derived expression. This demonstrates that cattle and sheep use different promoters to direct Cyp19 expression during pregnancy...
  14. doi request reprint Cultured bovine trophoblast cells differentially express genes encoding key steroid synthesis enzymes
    J Vanselow
    Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere, Wilhelm Stahl Allee 2, D 18196 Dummerstorf, Germany
    Placenta 29:531-8. 2008
    ..The cells represent an in vitro model to study the regulation of key genes of placental progesterone and androgen but not of oestrogen biosynthesis...
  15. ncbi request reprint Promoter-2-derived Cyp19 expression in bovine granulosa cells coincides with gene-specific DNA hypo-methylation
    J Vanselow
    Research Institute for the Biology of Farm Animals, Research Unit Molecular Biology, Wilhelm Stahl Allee 2, 18196 Dummerstorf, Germany
    Mol Cell Endocrinol 233:57-64. 2005
    ..The data suggest that promoter-2-derived high-level expression but not promoter-1.1-derived low-level expression of Cyp19 might be controlled by cell-type-specific DNA methylation...
  16. ncbi request reprint An aromatase pseudogene is transcribed in the bovine placenta
    R Fürbass
    Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere, Dummerstorf, Germany
    Gene 154:287-91. 1995
    ..The experiments demonstrate that the bovine genome contains a non-functional copy (pseudogene, Cyp19 phi) of bCyp19 that is transcribed together with the bCyp19 messenger in the bovine post-parturition placenta...
  17. ncbi request reprint Tissue-specific expression of the bovine aromatase-encoding gene uses multiple transcriptional start sites and alternative first exons
    R Fürbass
    Research Institute for the Biology of Farm Animals, Dummerstorf, Germany
    Endocrinology 138:2813-9. 1997
    ..These transcripts are presumably generated using five different promoter regions and tissue-specific alternative splicing...
  18. doi request reprint The mycotoxins alternariol and alternariol methyl ether negatively affect progesterone synthesis in porcine granulosa cells in vitro
    U Tiemann
    Research Institute for the Biology of Farm Animals, Dummerstorf, Germany
    Toxicol Lett 186:139-45. 2009
    ..In conclusion, AOH and AME, but not TeA specifically inhibited P(4) secretion in cultured porcine granulosa cells. Alternaria toxin contaminated food may therefore affect reproductive performance in pig and other mammalian species...
  19. ncbi request reprint Chromatin structure of the bovine Cyp19 promoter 1.1. DNaseI hypersensitive sites and DNA hypomethylation correlate with placental expression
    R Fürbass
    Research Unit Molecular Biology, Research Institute for the Biology of Farm Animals, Dummerstorf, Germany
    Eur J Biochem 268:1222-7. 2001
    ..In conclusion, observed tissue-specific differences of the chromatin structure which correlate with tissue-specific promoter activity suggest that chromatin might be an important regulator of aromatase expression in cattle...
  20. ncbi request reprint In vitro exposure of porcine granulosa cells to the phytoestrogens genistein and daidzein: effects on the biosynthesis of reproductive steroid hormones
    U Tiemann
    Unit of Reproductive Biology, Research Institute for the Biology of Farm Animals, 18196, Dummerstorf, Wilhelm Stahl Allee 2, Germany
    Reprod Toxicol 24:317-25. 2007
    ..In summary, genistein and daidzein have direct effects on porcine granulosa cell progesterone synthesis which involve the inhibition of 3beta-HSD enzyme activity across the post-cyclic AMP pathway...
  21. doi request reprint Feeding of pregnant sows with mycotoxin-contaminated diets and their non-effect on foetal and maternal hepatic transcription of genes of the insulin-like growth factor system
    U Tiemann
    Research Unit Reproductive Biology, Research Institute for the Biology of Farm Animals FBN, Dummerstorf, Germany
    Food Addit Contam Part A Chem Anal Control Expo Risk Assess 25:1365-73. 2008
    ..This suggests that mycotoxin-contaminated diets can induce growth depression in pigs during pregnancy without affecting hepatic transcription of major IGF genes...
  22. ncbi request reprint Novel aromatase transcripts from bovine placenta contain repeated sequence motifs
    J Vanselow
    Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere, Dummerstorf, Germany
    Gene 154:281-6. 1995
    ....
  23. ncbi request reprint Influence of Fusarium-toxin contaminated feed on initial quality and meiotic competence of gilt oocytes
    H Alm
    Department of Reproductive Biology, Research Institute for the Biology of Farm Animals, Dummerstorf, Germany
    Reprod Toxicol 22:44-50. 2006
    ..We conclude that oocyte quality is significantly reduced by feeding of Fusarium-toxins to gilts...
  24. ncbi request reprint Effects of the mycotoxins alpha- and beta-zearalenol on regulation of progesterone synthesis in cultured granulosa cells from porcine ovaries
    U Tiemann
    Unit of Reproductive Biology, Research Institute for the Biology of Farm Animals, 18196, Wilhelm Stahl Allee 2, Dummerstorf, Germany
    Reprod Toxicol 17:673-81. 2003
    ..The apparent dose-dependent reduction of p450scc and 3beta-HSD transcripts implies an effect of alpha- and beta-ZOL on transcriptional regulation of these enzymes...
  25. ncbi request reprint Genomic organization of the bovine aromatase encoding gene and a homologous pseudogene as revealed by DNA fiber FISH
    R M Brunner
    Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere Dummerstorf, Dummerstorf, Germany
    Cytogenet Cell Genet 82:37-40. 1998
    ..3) The physical size of the bovine CYP19 locus amounts to a minimum of 130 kb...
  26. ncbi request reprint Maternal treatment with somatotropin during early gestation affects basic events of myogenesis in pigs
    C Rehfeldt
    Division of Muscle Biology and Growth, Research Institute for the Biology of Farm Animals, Wilhelm Stahl Allee 2, 18196 Dummerstorf, Germany
    Cell Tissue Res 306:429-40. 2001
    ..In conclusion, maternal somatotropin is an influential factor in early pregnancy capable of affecting the basic events of myogenesis...
  27. ncbi request reprint Interleukin-12 p35 encoding gene of cattle and sheep harbours a polymorphic T stretch in intron 4
    P Schmidt
    Research Institute for the Biology of Farm Animals, Research Unit of Molecular Biology, Dummerstorf, Germany
    Anim Genet 31:283-5. 2000
  28. doi request reprint Down-regulation of genes encoding steroidogenic enzymes and hormone receptors in late preovulatory follicles of the cow coincides with an accumulation of intrafollicular steroids
    Marika Nimz
    Forschungsbereich Molekularbiologie, Forschungsinstitut für die Biologie landwirtschaftlicher Nutztiere, Wilhelm Stahl Allee 2, Dummerstorf, Germany
    Domest Anim Endocrinol 37:45-54. 2009
    ..Collectively, our data show that late preovulatory follicles have a transient and unique gene expression profile and are clearly different from both the preceding and subsequent (follicular and luteal, respectively) stages...
  29. ncbi request reprint DNA methylation and chromatin accessibility of the proximal Cyp 19 promoter region 1.5/2 correlate with expression levels in sheep placentomes
    Rainer Fürbass
    Research Unit Molecular Biology, Research Institute for the Biology of Farm Animals, Dummerstorf, Germany
    Mol Reprod Dev 75:1-7. 2008
    ..The results imply that DNA methylation and chromatin accessibility of the P1.5/2 promoter region correlate with expression levels of the Cyp 19 gene...
  30. ncbi request reprint Three different promoters control expression of the aromatase cytochrome p450 gene (cyp19) in mouse gonads and brain
    Konstantin Golovine
    Research Unit Molecular Biology, Research Institute for the Biology of Farm Animals, 18196 Dummerstorf, Germany
    Biol Reprod 68:978-84. 2003
    ..In brains of both sexes, T(br) was found to predominate throughout postnatal life. The results suggest that the mouse Cyp19 gene includes three different promoters that specifically direct expression in ovary, testis, and brain...
  31. ncbi request reprint DNA-remethylation around a STAT5-binding enhancer in the alphaS1-casein promoter is associated with abrupt shutdown of alphaS1-casein synthesis during acute mastitis
    Jens Vanselow
    Research Institute for the Biology of Farm Animals FBN, Wilhelm Stahl Allee 2, 18196 Dummerstorf, Germany
    J Mol Endocrinol 37:463-77. 2006
    ..This provides a rare example for an acute regulatory significance of CpG methylation...
  32. ncbi request reprint Two high-fertility mouse lines show differences in component fertility traits after long-term selection
    Marion Spitschak
    Forschungsbereich Molekularbiologie, Forschungsinstitut fur die Biologie landwirtschaftlicher Nutztiere FBN, Wilhelm Stahl Allee 2, Dummerstorf, Germany
    Reprod Fertil Dev 19:815-21. 2007
    ....
  33. ncbi request reprint Androstenedione increases cytochrome P450 aromatase messenger ribonucleic acid transcripts in nonluteinizing bovine granulosa cells
    Melanie Hamel
    Centre de Recherche en Reproduction Animale, Faculte de Medecine Veterinaire, Universite de Montreal, St Hyacinthe, Quebec J2S 7C6, Canada
    Mol Reprod Dev 70:175-83. 2005
    ..Interestingly, different androgens had different effects on Cyp19 promoter usage, suggesting differential regulation of aromatase gene expression in the developing follicle...
  34. ncbi request reprint Effects of galectin-1 on regulation of progesterone production in granulosa cells from pig ovaries in vitro
    Hermann Walzel
    University of Rostock, Institute of Medical Biochemistry and Molecularbiology, Schillingallee 70, 18057 Rostock, Germany
    Glycobiology 14:871-81. 2004
    ..We conclude that gal-1 exerts its inhibitory effect on steroidogenic activity of granulosa cells by interfering the hormone-receptor interaction resulting in decreased responses to FSH stimulation...
  35. ncbi request reprint Expression of the bovine aromatase cytochrome P450 gene (Cyp19) is primarily regulated by promoter 2 in bovine follicles and by promoter 1.1 in corpora lutea
    Solvig Lenz
    Department of Orthopedics and Orthopedic Surgery, University of Rostock, Rostock, Germany
    Mol Reprod Dev 67:406-13. 2004
    ....
  36. ncbi request reprint 0.2kb promoter sequence of the murine Cyp19 gene target beta-galactosidase expression to specific brain areas of transgenic mice
    Norman Nausch
    Division of Innate Immunity, German Cancer Research Center, Im Neuenheimer Feld 280, 69120 Heidelberg, Germany
    J Steroid Biochem Mol Biol 103:119-28. 2007
    ..2 and 1.7kb upstream, is necessary for expression in additional areas. However even 1.7kb of P(br) are not sufficient to consistently mimic the accurate expression pattern of Cyp19...