Ludger Beerhues

Summary

Affiliation: Technical University of Braunschweig
Country: Germany

Publications

  1. ncbi request reprint Hyperforin
    Ludger Beerhues
    Institut fur Pharmazeutische Biologie, Technische Universitat Braunschweig, Mendelssohnstr 1, D 38106 Braunschweig, Germany
    Phytochemistry 67:2201-7. 2006
  2. pmc Phytoalexins of the Pyrinae: Biphenyls and dibenzofurans
    Cornelia Chizzali
    Institut fur Pharmazeutische Biologie, Technische Universitat Braunschweig, Mendelssohnstr 1, 38106 Braunschweig, Germany
    Beilstein J Org Chem 8:613-20. 2012
  3. doi request reprint Biosynthesis of biphenyls and benzophenones--evolution of benzoic acid-specific type III polyketide synthases in plants
    Ludger Beerhues
    Institut fur Pharmazeutische Biologie, Technische Universitat Braunschweig, Braunschweig, Germany
    Phytochemistry 70:1719-27. 2009
  4. pmc Cinnamate:CoA ligase initiates the biosynthesis of a benzoate-derived xanthone phytoalexin in Hypericum calycinum cell cultures
    Mariam M Gaid
    Institute of Pharmaceutical Biology, Technische Universitat Braunschweig, 38106 Braunschweig, Germany
    Plant Physiol 160:1267-80. 2012
  5. doi request reprint Biosynthesis of the biphenyl phytoalexin aucuparin in Sorbus aucuparia cell cultures treated with Venturia inaequalis
    Mohammed N A Khalil
    Institute of Pharmaceutical Biology, Technische Universitat Braunschweig, Mendelssohnstr 1, 38106 Braunschweig, Germany
    Phytochemistry 96:101-9. 2013
  6. doi request reprint 4-Coumarate:CoA ligase family members from elicitor-treated Sorbus aucuparia cell cultures
    Mariam M Gaid
    Institut fur Pharmazeutische Biologie, Technische Universitat Braunschweig, Mendelssohnstraße 1, D 38106 Braunschweig, Germany
    J Plant Physiol 168:944-51. 2011
  7. doi request reprint Formation of biphenyl and dibenzofuran phytoalexins in the transition zones of fire blight-infected stems of Malus domestica cv. 'Holsteiner Cox' and Pyrus communis cv. 'Conference'
    Cornelia Chizzali
    Institute of Pharmaceutical Biology, Technische Universitat Braunschweig, Mendelssohnstr 1, 38106 Braunschweig, Germany
    Phytochemistry 77:179-85. 2012
  8. ncbi request reprint Biosynthesis of the hyperforin skeleton in Hypericum calycinum cell cultures
    Petra Klingauf
    Institut fur Pharmazeutische Biologie, Technische Universitat Braunschweig, Mendelssohnstrasse 1, D 38106 Braunschweig, Germany
    Phytochemistry 66:139-45. 2005
  9. ncbi request reprint The first prenylation step in hyperforin biosynthesis
    Zakia Boubakir
    Institut fuer Pharmazeutische Biologie, Technische Universitaet Braunschweig, Mendelssohnstr 1, D 38106 Braunschweig, Germany
    Phytochemistry 66:51-7. 2005
  10. pmc A single amino acid substitution converts benzophenone synthase into phenylpyrone synthase
    Tim Klundt
    Institute of Pharmaceutical Biology, Technical University of Braunschweig, Mendelssohnstrasse 1, D 38106 Braunschweig, Germany
    J Biol Chem 284:30957-64. 2009

Collaborators

  • Mariam Gaid
  • Debabrata Sircar
  • Klaus Richter
  • Wolfgang Brandt
  • Thilo C Fischer
  • Sönke Behrends
  • G Bringmann
  • Benye Liu
  • Till Beuerle
  • Cornelia Chizzali
  • Mohammed N A Khalil
  • Ludger Ernst
  • Islam El-Awaad
  • Zakia Boubakir
  • Paul Haas
  • Tim Klundt
  • Tobias Fiesel
  • Andreas Muller
  • Robert Hansch
  • Iman A M Abdel-Rahman
  • Marco Bocola
  • Andreas Peil
  • Magda Viola Hanke
  • Henryk Flachowsky
  • Cornelia Hüttner
  • Armen Charchoglyan
  • Petra Klingauf
  • Ajmal Zarinwall
  • Stephan Scholl
  • Sahar Abdelaziz
  • Jana Halusková
  • Asya K Swiddan
  • Josephine Groeneveld
  • Dennis Reckwell
  • Joana Bartels
  • Ahmed M A Abd El-Mawla
  • Vijaya B R Bhavanam
  • Afaf M Abdel-baky
  • Amany S Ahmed
  • Ezz El Din K Desoky
  • Asma K Belkheir
  • Wolfgang Schuehly
  • Helge Scharnhop
  • Torben Raeth
  • Maren Lütge
  • Toni M Kutchan
  • Yutaka Ebizuka
  • Arusyak Abrahamyan
  • Isao Fujii
  • Tobias A M Gulder
  • Hrachik Vardapetyan
  • Safaa A M El-Moghazy
  • Annett Mellenthin
  • Hildegard Falkenstein-Paul
  • Werner Schmidt

Detail Information

Publications27

  1. ncbi request reprint Hyperforin
    Ludger Beerhues
    Institut fur Pharmazeutische Biologie, Technische Universitat Braunschweig, Mendelssohnstr 1, D 38106 Braunschweig, Germany
    Phytochemistry 67:2201-7. 2006
    ..The first prenylation step is catalyzed by a soluble and ion-dependent dimethylallyltransferase. Hyperforin mainly accumulates in pistils and fruits where it probably serves as defensive compound...
  2. pmc Phytoalexins of the Pyrinae: Biphenyls and dibenzofurans
    Cornelia Chizzali
    Institut fur Pharmazeutische Biologie, Technische Universitat Braunschweig, Mendelssohnstr 1, 38106 Braunschweig, Germany
    Beilstein J Org Chem 8:613-20. 2012
    ..Metabolism of the phytoalexins may provide new tools for designing disease control strategies for fruit trees of the Pyrinae subtribe...
  3. doi request reprint Biosynthesis of biphenyls and benzophenones--evolution of benzoic acid-specific type III polyketide synthases in plants
    Ludger Beerhues
    Institut fur Pharmazeutische Biologie, Technische Universitat Braunschweig, Braunschweig, Germany
    Phytochemistry 70:1719-27. 2009
    ..The functional behavior of this Thr135Leu mutant was rationalized by homology modeling. The intermediate triketide may be redirected into a smaller pocket in the active site cavity, resulting in phenylpyrone formation by lactonization...
  4. pmc Cinnamate:CoA ligase initiates the biosynthesis of a benzoate-derived xanthone phytoalexin in Hypericum calycinum cell cultures
    Mariam M Gaid
    Institute of Pharmaceutical Biology, Technische Universitat Braunschweig, 38106 Braunschweig, Germany
    Plant Physiol 160:1267-80. 2012
    ..The other cluster comprised 4-coumarate:CoA ligases from spermatophytes, ferns, and mosses, indicating divergence of the two clades prior to the divergence of the higher plant lineages...
  5. doi request reprint Biosynthesis of the biphenyl phytoalexin aucuparin in Sorbus aucuparia cell cultures treated with Venturia inaequalis
    Mohammed N A Khalil
    Institute of Pharmaceutical Biology, Technische Universitat Braunschweig, Mendelssohnstr 1, 38106 Braunschweig, Germany
    Phytochemistry 96:101-9. 2013
    ..Nevertheless, a basis for studying biphenyl biosynthesis at the gene level is provided. ..
  6. doi request reprint 4-Coumarate:CoA ligase family members from elicitor-treated Sorbus aucuparia cell cultures
    Mariam M Gaid
    Institut fur Pharmazeutische Biologie, Technische Universitat Braunschweig, Mendelssohnstraße 1, D 38106 Braunschweig, Germany
    J Plant Physiol 168:944-51. 2011
    ..Phylogenetically, Sa4CL1 and Sa4CL2 belong to the class I cluster and Sa4CL3 groups in the class II cluster. Sa4CL3 contains a 49-amino acid N-terminal extension, which includes a chloroplast sorting signal...
  7. doi request reprint Formation of biphenyl and dibenzofuran phytoalexins in the transition zones of fire blight-infected stems of Malus domestica cv. 'Holsteiner Cox' and Pyrus communis cv. 'Conference'
    Cornelia Chizzali
    Institute of Pharmaceutical Biology, Technische Universitat Braunschweig, Mendelssohnstr 1, 38106 Braunschweig, Germany
    Phytochemistry 77:179-85. 2012
    ..The most efficient compound was 3,5-dihydroxybiphenyl (MIC=115 μg/ml), the immediate product of biphenyl synthase which initiates phytoalexin biosynthesis...
  8. ncbi request reprint Biosynthesis of the hyperforin skeleton in Hypericum calycinum cell cultures
    Petra Klingauf
    Institut fur Pharmazeutische Biologie, Technische Universitat Braunschweig, Mendelssohnstrasse 1, D 38106 Braunschweig, Germany
    Phytochemistry 66:139-45. 2005
    ..The three type III polyketide synthases were separated by anion exchange chromatography...
  9. ncbi request reprint The first prenylation step in hyperforin biosynthesis
    Zakia Boubakir
    Institut fuer Pharmazeutische Biologie, Technische Universitaet Braunschweig, Mendelssohnstr 1, D 38106 Braunschweig, Germany
    Phytochemistry 66:51-7. 2005
    ..The formation of hyperforins in H. calycinum cell cultures was preceded by an increase in dimethylallyltransferase activity, with the maximum specific activity being 3.6 microkat/kg protein...
  10. pmc A single amino acid substitution converts benzophenone synthase into phenylpyrone synthase
    Tim Klundt
    Institute of Pharmaceutical Biology, Technical University of Braunschweig, Mendelssohnstrasse 1, D 38106 Braunschweig, Germany
    J Biol Chem 284:30957-64. 2009
    ..PPS is a promising biotechnological tool for manipulating benzoate-primed biosynthetic pathways to produce novel compounds...
  11. doi request reprint Benzaldehyde dehydrogenase from chitosan-treated Sorbus aucuparia cell cultures
    Mariam M Gaid
    Institute of Pharmaceutical Biology, Technical University of Braunschweig, Mendelssohnstrasse 1, D 38106 Braunschweig, Germany
    J Plant Physiol 166:1343-9. 2009
    ..Cinnamaldehyde and various hydroxybenzaldehydes were relatively poor substrates. BD activity was strictly dependent on the presence of NAD(+) as a cofactor (K(m)=67 microM)...
  12. pmc Biphenyl 4-Hydroxylases Involved in Aucuparin Biosynthesis in Rowan and Apple Are Cytochrome P450 736A Proteins
    Debabrata Sircar
    Institute of Pharmaceutical Biology D S, M M G, D R, T B, B L, L B and Institute of Plant Biology D K, R H, Technische Universitat Braunschweig, 38106 Braunschweig, Germany Plant Pathology, Institute of Integrative Biology, Eidgenossische Technische Hochschule Zurich, 8092 Zurich, Switzerland C C, G A L B andJulius Kühn Institute, Federal Research Centre for Cultivated Plants, Institute for Breeding Research on Horticultural and Fruit Crops, 01326 Dresden, Germany H F
    Plant Physiol 168:428-42. 2015
    ..In a phylogenetic tree, the three B4Hs were closest to coniferaldehyde 5-hydroxylases involved in lignin biosynthesis, suggesting a common ancestor. Coniferaldehyde and related compounds were not converted by SaB4H. ..
  13. doi request reprint O-Methyltransferases involved in biphenyl and dibenzofuran biosynthesis
    Mohammed N A Khalil
    Institute of Pharmaceutical Biology, Technische Universitat Braunschweig, Mendelssohnstr 1, 38106, Braunschweig, Germany
    Plant J 83:263-76. 2015
    ..All the fluorescent reporter fusions were localized to the cytoplasm of Nicotiana benthamiana leaf epidermis cells. A revised biosynthetic pathway of biphenyls and dibenzofurans in the Malinae is presented. ..
  14. doi request reprint A novel 4-hydroxycoumarin biosynthetic pathway
    Benye Liu
    Institut fur Pharmazeutische Biologie, Mendelssohnstrasse 1, 38106, Braunschweig, Germany
    Plant Mol Biol 72:17-25. 2010
    ..Incubation of the BIS isoenzymes with benzoyl-CoA and malonyl-CoA resulted in the formation of 3,5-dihydroxybiphenyl which is the precursor of the phytoalexins of the Maloideae...
  15. pmc Differential expression of biphenyl synthase gene family members in fire-blight-infected apple 'Holsteiner Cox'
    Cornelia Chizzali
    Institut fur Pharmazeutische Biologie, Technische Universitat Braunschweig, 38106 Braunschweig, Germany
    Plant Physiol 158:864-75. 2012
    ..Dot-shaped immunofluorescence was confined to the junctions between neighboring cortical parenchyma cells...
  16. doi request reprint Hyperforin production in Hypericum perforatum root cultures
    Mariam Gaid
    Institute of Pharmaceutical Biology, Technische Universitat Braunschweig, Braunschweig, Germany Center of Pharmaceutical Engineering, Technische Universitat Braunschweig, Braunschweig, Germany Electronic address
    J Biotechnol 222:47-55. 2016
    ..Upon up-scaling of biomass production and downstream processing, H. perforatum root cultures may provide an alternative platform for the preparation of medicinal extracts and the isolation of APIs...
  17. doi request reprint In vitro formation of the anthranoid scaffold by cell-free extracts from yeast-extract-treated Cassia bicapsularis cell cultures
    Iman A M Abdel-Rahman
    Institute of Pharmaceutical Biology, Technische Universitat Braunschweig, Mendelssohnstrasse 1, 38106 Braunschweig, Germany
    Phytochemistry 88:15-24. 2013
    ..Detection of anthranoid biosynthesis in cell-free extracts indicates in vitro cooperation of OKS with a yet unidentified factor or enzyme for octaketide cyclization...
  18. ncbi request reprint Benzophenone synthase and chalcone synthase from Hypericum androsaemum cell cultures: cDNA cloning, functional expression, and site-directed mutagenesis of two polyketide synthases
    Benye Liu
    Institut fur Pharmazeutische Biologie, Mendelssohnstrasse 1, D 38106 Braunschweig, Germany
    Plant J 34:847-55. 2003
    ..Site-directed mutagenesis of amino acids shaping the initiation/elongation cavity of CHS yielded a triple mutant (L263M/F265Y/S338G) that preferred benzoyl-CoA over 4-coumaroyl-CoA...
  19. pmc Bifunctional CYP81AA proteins catalyse identical hydroxylations but alternative regioselective phenol couplings in plant xanthone biosynthesis
    Islam El-Awaad
    Institute of Pharmaceutical Biology, Technische Universitat Braunschweig, Mendelssohnstraße 1, Braunschweig 38106, Germany
    Nat Commun 7:11472. 2016
    ..However, the reciprocal mutations in HpCYP81AA1 barely affect its regiospecificity. Product docking rationalizes the alternative C-O phenol coupling reactions. Our results help understand the machinery of bifunctional CYPs. ..
  20. pmc Expression of Biphenyl Synthase Genes and Formation of Phytoalexin Compounds in Three Fire Blight-Infected Pyrus communis Cultivars
    Cornelia Chizzali
    Institute of Pharmaceutical Biology, Technische Universitat Braunschweig, Mendelssohnstr 1, 38106, Braunschweig, Germany
    PLoS ONE 11:e0158713. 2016
    ..In contrast, the transition zones of 'Alexander Lucas' and 'Conference' advanced down to the rootstock, resulting in necrosis of the entire shoots. ..
  21. doi request reprint Differential effect of elicitors on biphenyl and dibenzofuran formation in Sorbus aucuparia cell cultures
    Cornelia Hüttner
    Institute of Pharmaceutical Biology, Technische Universität Braunschweig, Mendelssohnstrasse 1, 38106 Braunschweig, Germany
    J Agric Food Chem 58:11977-84. 2010
    ..Elicitor-treated S. aucuparia cell cultures provide an interesting in vitro system for studying biphenyl and dibenzofuran metabolism in the economically valuable Pyrinae...
  22. doi request reprint Downstream processing of hyperforin from Hypericum perforatum root cultures
    Paul Haas
    Institute for Chemical and Thermal Process Engineering, Technische Universitat Braunschweig, Germany Center of Pharmaceutical Engineering, Technische Universitat Braunschweig, Germany
    Eur J Pharm Biopharm . 2017
    ..3mg hyperforin/g dry weight bio material. Parameters for the equipment design for extraction and solvent evaporation were determined based on the experimental data...
  23. doi request reprint Molecular Cloning and Characterization of a Xanthone Prenyltransferase from Hypericum calycinum Cell Cultures
    Tobias Fiesel
    Institute of Pharmaceutical Biology, Technische Universitat Braunschweig, Mendelssohnstraße 1, Braunschweig 38106, Germany
    Molecules 20:15616-30. 2015
    ..Thus, our findings may offer a chance for semisynthesis of new active agents to be involved in the treatment of Alzheimer's disease. ..
  24. ncbi request reprint Biphenyl synthase from yeast-extract-treated cell cultures of Sorbus aucuparia
    Benye Liu
    Institut fur Pharmazeutische Biologie, Technische Universitat Braunschweig, Mendelssohnstrasse 1, 38106 Braunschweig, Germany
    Planta 218:492-6. 2004
    ..Relatively high activity was also observed with 2-hydroxybenzoyl-CoA but, instead of the corresponding biphenyl, the derailment product 2-hydroxybenzoyltriacetic acid lactone was formed...
  25. ncbi request reprint Benzoic acid biosynthesis in cell cultures of Hypericum androsaemum
    Ahmed M A Abd El-Mawla
    Institut fur Pharmazeutische Biologie, Braunschweig, Germany
    Planta 214:727-33. 2002
    ..Similar changes in activity were observed for phenylalanine ammonia-lyase and the xanthone biosynthetic enzymes benzoate:CoA ligase and benzophenone synthase...
  26. ncbi request reprint Differential accumulation of hyperforin and secohyperforin in Hypericum perforatum tissue cultures
    Armen Charchoglyan
    Yerevan State University, Faculty of Biology, Alex Manoogian Street 1, Yerevan, Armenia
    Phytochemistry 68:2670-7. 2007
    ..No differential stimulation was observed after elicitor treatment. Hyperforin and secohyperforin are proposed to arise from a branch point in the biosynthetic pathway...
  27. ncbi request reprint RDNA methylation in Hypericum perforatum diploids and tetraploids
    Jana Halusková
    Institute of Biology and Ecology, Faculty of Science, P J Safarik University, Manesova 23, 04167 Kosice, Slovakia
    Z Naturforsch C 58:895-7. 2003
    ..No differences in rDNA methylation were observed between diploids and tetraploids at an early ontogenetic stage...