Ralph Witzgall

Summary

Affiliation: University of Regensburg
Country: Germany

Publications

  1. ncbi request reprint TRPP2 channel regulation
    R Witzgall
    Institute for Molecular and Cellular Anatomy, University of Regensburg, Universitatsstrasse 31, 93053 Regensburg, Germany
    Handb Exp Pharmacol . 2007
  2. pmc How are podocytes affected in nail-patella syndrome?
    Ralph Witzgall
    Pediatr Nephrol 23:1017-20. 2008
  3. pmc Mutant polycystin-2 induces proliferation in primary rat tubular epithelial cells in a STAT-1/p21-independent fashion accompanied instead by alterations in expression of p57KIP2 and Cdk2
    Kyriacos N Felekkis
    Department of Biological Sciences, University of Cyprus, Cyprus
    BMC Nephrol 9:10. 2008
  4. ncbi request reprint New developments in the field of cystic kidney diseases
    Ralph Witzgall
    University of Regensburg, Institute for Molecular and Cellular Anatomy, Germany
    Curr Mol Med 5:455-65. 2005
  5. ncbi request reprint Polycystin-2--an intracellular or plasma membrane channel?
    Ralph Witzgall
    Institute for Molecular and Cellular Anatomy, University of Regensburg, Universitatsstrasse 31, 93053, Regensburg, Germany
    Naunyn Schmiedebergs Arch Pharmacol 371:342-7. 2005
  6. doi request reprint The LIM-homeodomain transcription factor LMX1B regulates expression of NF-kappa B target genes
    Anne Rascle
    Institute for Molecular and Cellular Anatomy, University of Regensburg, Universitaetsstrasse 31, 93053 Regensburg, Germany
    Exp Cell Res 315:76-96. 2009
  7. pmc Polycystin-2 takes different routes to the somatic and ciliary plasma membrane
    Helen Hoffmeister
    Institute for Molecular and Cellular Anatomy, University of Regensburg, 93053 Regensburg, Germany
    J Cell Biol 192:631-45. 2011
  8. doi request reprint Role of the Ca2+ -activated Cl- channels bestrophin and anoctamin in epithelial cells
    Karl Kunzelmann
    Institut fur Physiologie, Universitat Regensburg, Universitatsstrasse 31, Regensburg, Germany
    Biol Chem 392:125-34. 2011
  9. doi request reprint The human polycystin-2 protein represents an integral membrane protein with six membrane-spanning domains and intracellular N- and C-termini
    Helen Hoffmeister
    Institute for Molecular and Cellular Anatomy, University of Regensburg, Germany
    Biochem J 433:285-94. 2011
  10. doi request reprint Doxycycline accelerates renal cyst growth and fibrosis in the pcy/pcy mouse model of type 3 nephronophthisis, a form of recessive polycystic kidney disease
    Larissa Osten
    Institute for Molecular and Cellular Anatomy, University of Regensburg, Universitatsstrasse 31, 93053, Regensburg, Germany
    Histochem Cell Biol 132:199-210. 2009

Collaborators

Detail Information

Publications31

  1. ncbi request reprint TRPP2 channel regulation
    R Witzgall
    Institute for Molecular and Cellular Anatomy, University of Regensburg, Universitatsstrasse 31, 93053 Regensburg, Germany
    Handb Exp Pharmacol . 2007
    ..This section irst introduces the reader to the field of cystic kidney diseases and to the PKD2 gene, before the ion channel properties of polycystin-2 are discussed in great detail...
  2. pmc How are podocytes affected in nail-patella syndrome?
    Ralph Witzgall
    Pediatr Nephrol 23:1017-20. 2008
    ..LMX1B can serve as a model system to elucidate a genetic program in podocytes...
  3. pmc Mutant polycystin-2 induces proliferation in primary rat tubular epithelial cells in a STAT-1/p21-independent fashion accompanied instead by alterations in expression of p57KIP2 and Cdk2
    Kyriacos N Felekkis
    Department of Biological Sciences, University of Cyprus, Cyprus
    BMC Nephrol 9:10. 2008
    ....
  4. ncbi request reprint New developments in the field of cystic kidney diseases
    Ralph Witzgall
    University of Regensburg, Institute for Molecular and Cellular Anatomy, Germany
    Curr Mol Med 5:455-65. 2005
    ..It is speculated that primary cilia and their associated proteins play a role in determining the proper tubular geometry...
  5. ncbi request reprint Polycystin-2--an intracellular or plasma membrane channel?
    Ralph Witzgall
    Institute for Molecular and Cellular Anatomy, University of Regensburg, Universitatsstrasse 31, 93053, Regensburg, Germany
    Naunyn Schmiedebergs Arch Pharmacol 371:342-7. 2005
    ..PIGEA-14 also interacts with GM130, a protein associated with the Golgi matrix, and may therefore represent one important component of the trafficking machinery for polycystin-2...
  6. doi request reprint The LIM-homeodomain transcription factor LMX1B regulates expression of NF-kappa B target genes
    Anne Rascle
    Institute for Molecular and Cellular Anatomy, University of Regensburg, Universitaetsstrasse 31, 93053 Regensburg, Germany
    Exp Cell Res 315:76-96. 2009
    ..Together, these data demonstrate for the first time that LMX1B directly regulates transcription of a subset of NF-kappaB target genes in cooperation with nuclear p50/p65 NF-kappaB...
  7. pmc Polycystin-2 takes different routes to the somatic and ciliary plasma membrane
    Helen Hoffmeister
    Institute for Molecular and Cellular Anatomy, University of Regensburg, 93053 Regensburg, Germany
    J Cell Biol 192:631-45. 2011
    ....
  8. doi request reprint Role of the Ca2+ -activated Cl- channels bestrophin and anoctamin in epithelial cells
    Karl Kunzelmann
    Institut fur Physiologie, Universitat Regensburg, Universitatsstrasse 31, Regensburg, Germany
    Biol Chem 392:125-34. 2011
    ..To fully understand the function and regulation of Ca(2+)-activated Cl(-) currents, it is necessary to appreciate that Best1 and TMEM16A are embedded in a protein network and that they probably operate in functional microdomains...
  9. doi request reprint The human polycystin-2 protein represents an integral membrane protein with six membrane-spanning domains and intracellular N- and C-termini
    Helen Hoffmeister
    Institute for Molecular and Cellular Anatomy, University of Regensburg, Germany
    Biochem J 433:285-94. 2011
    ..The results of the present study therefore provide the first experimental evidence for the topological orientation of polycystin-2...
  10. doi request reprint Doxycycline accelerates renal cyst growth and fibrosis in the pcy/pcy mouse model of type 3 nephronophthisis, a form of recessive polycystic kidney disease
    Larissa Osten
    Institute for Molecular and Cellular Anatomy, University of Regensburg, Universitatsstrasse 31, 93053, Regensburg, Germany
    Histochem Cell Biol 132:199-210. 2009
    ..Our data also add a note of caution when interpreting phenotypes of animal models based upon the tet system...
  11. pmc Ca2+-dependent conformational changes in a C-terminal cytosolic domain of polycystin-2
    Frank Schumann
    Institute of Biophysics and Physical Biochemistry, University of Regensburg, Regensburg, Germany
    J Biol Chem 284:24372-83. 2009
    ..The calcium affinity of the protein was determined by fluorescence and NMR spectroscopy. At 293 K, the K(D) values for the high and low affinity sites are 55 mum and 179 mum, respectively...
  12. doi request reprint NMR-assignments of a cytosolic domain of the C-terminus of polycystin-2
    Frank H Schumann
    Institute of Biophysics and Physical Biochemistry, University of Regensburg, 93040, Regensburg, Germany
    Biomol NMR Assign 3:141-4. 2009
    ..The chemical shifts obtained are in line with the existence of two paired helix-loop-helix (HLH) motifs...
  13. ncbi request reprint A truncated polycystin-2 protein causes polycystic kidney disease and retinal degeneration in transgenic rats
    Anna Rachel Gallagher
    Institute for Molecular and Cellular Anatomy, University of Regensburg, Universitatsstrasse 31, 93053 Regensburg, Germany
    J Am Soc Nephrol 17:2719-30. 2006
    ..Other experimental approaches, such as a knock-in strategy, will be necessary to validate these results, but this is the first preliminary evidence that cyst formation is due not only to somatic mutations...
  14. doi request reprint ER-localized bestrophin 1 activates Ca2+-dependent ion channels TMEM16A and SK4 possibly by acting as a counterion channel
    Rene Barro-Soria
    Institut fur Physiologie, Universitat Regensburg, Universitatsstrasse 31, 93053 Regensburg, Germany
    Pflugers Arch 459:485-97. 2010
    ..Thus, bestrophin 1 regulates compartmentalized Ca(2+) signaling that plays an essential role in Best macular dystrophy, inflammatory diseases such as cystic fibrosis, as well as proliferation...
  15. ncbi request reprint The podocyte-specific inactivation of Lmx1b, Ldb1 and E2a yields new insight into a transcriptional network in podocytes
    Hani Suleiman
    University of Regensburg, Institute for Molecular and Cellular Anatomy, Universitätsstr 31, 93053 Regensburg, and Division of Nephrology and Immunology, University Hospital, RWTH, Aachen, Germany
    Dev Biol 304:701-12. 2007
    ..We conclude that LDB1, but not E2A is a promising candidate as a modifier gene in patients with nail-patella syndrome...
  16. ncbi request reprint Lack of connexin 40 causes displacement of renin-producing cells from afferent arterioles to the extraglomerular mesangium
    Lisa Kurtz
    Physiologisches Institut der Universität Regensburg, D 93040 Regensburg, Germany
    J Am Soc Nephrol 18:1103-11. 2007
    ..Moreover, these findings support the notion that gap junctions are relevant for the macula densa signaling to renin-producing cells...
  17. doi request reprint Calmodulin-dependent activation of the epithelial calcium-dependent chloride channel TMEM16A
    Yuemin Tian
    Institut fur Physiologie, Universitat Regensburg, Universitätsstraße 31, D 93053 Regensburg, Germany
    FASEB J 25:1058-68. 2011
    ..These results reinforce the use of these compounds for activation of electrolyte secretion in diseases such as cystic fibrosis...
  18. ncbi request reprint Role of transcription factors in podocytes
    Anne Rascle
    Institute for Molecular and Cellular Anatomy, University of Regensburg, Regensburg, Germany
    Nephron Exp Nephrol 106:e60-6. 2007
    ..Other transcription factors such as hypoxia-inducible factors and PAX2 are likely to play a role in podocytes, whereas the significance of others, e.g. of POD1 and CITED2, is more speculative at this point...
  19. doi request reprint Advanced electron microscopic techniques provide a deeper insight into the peculiar features of podocytes
    Tillmann Burghardt
    Institute for Molecular and Cellular Anatomy, University of Regensburg, Regensburg, Germany
    Am J Physiol Renal Physiol 309:F1082-9. 2015
    ..Future work will be necessary to determine the proteins constituting the two types of cell-cell contacts. ..
  20. ncbi request reprint PIGEA-14, a novel coiled-coil protein affecting the intracellular distribution of polycystin-2
    Sumi Hidaka
    Institute for Molecular and Cellular Anatomy, University of Regensburg, Universitatsstrasse 31, 93053 Regensburg, Germany
    J Biol Chem 279:35009-16. 2004
    ..Our results also indicate that the intracellular trafficking of polycystin-2 is regulated both at the level of the endo-plasmic reticulum and that of the trans-Golgi network...
  21. doi request reprint The calcium-activated chloride channel Anoctamin 1 contributes to the regulation of renal function
    Diana Faria
    Universitat Regensburg, Institute für Physiologie, Regensburg, Germany
    Kidney Int 85:1369-81. 2014
    ..Thus, Ano1 has a role in proximal tubular proton secretion and protein reabsorption. The results correspond to regulation of the proton-ATPase by the Ano1-homolog Ist2 in yeast. ..
  22. doi request reprint Kidney podocytes as specific targets for cyclo(RGDfC)-modified nanoparticles
    Klaus Pollinger
    Department of Pharmaceutical Technology, University of Regensburg, Universitätsstr 31, 93053 Regensburg, Germany
    Small 8:3368-75. 2012
    ..This could be a highly promising approach for future therapy and diagnostics of podocyte-associated diseases...
  23. pmc KCNJ10 gene mutations causing EAST syndrome (epilepsy, ataxia, sensorineural deafness, and tubulopathy) disrupt channel function
    Markus Reichold
    Department of Physiology, University of Regensburg, 93053 Regensburg, Germany
    Proc Natl Acad Sci U S A 107:14490-5. 2010
    ..Intriguingly, the metabolic alkalosis present in patients carrying the R65P mutation possibly improves residual function of KCNJ10, which shows higher activity at alkaline pH...
  24. ncbi request reprint Lack of a laterality phenotype in Pkd1 knock-out embryos correlates with absence of polycystin-1 in nodal cilia
    Christina Karcher
    Institut für Zoologie 220, Universitat Hohenheim, Garbenstr 30, D 70593 Stuttgart, Germany
    Differentiation 73:425-32. 2005
    ..Our results demonstrate a PC1-independent function of PC2 in left-right axis formation, and indirectly support a ciliary role of PC2 in this process...
  25. ncbi request reprint Nonspecific cation current associated with native polycystin-2 in HEK-293 cells
    Bruna Pelucchi
    Department of Biology, University of Ferrara, Via L Borsari 46, Ferrara I 44100, Italy
    J Am Soc Nephrol 17:388-97. 2006
    ....
  26. ncbi request reprint Missense mutation in sterile alpha motif of novel protein SamCystin is associated with polycystic kidney disease in (cy/+) rat
    Joanna H Brown
    The Wellcome Trust Centre for Human Genetics, University of Oxford, Oxford, UK
    J Am Soc Nephrol 16:3517-26. 2005
    ..The characterization of this protein, which does not share structural homologies with known polycystins, may give new insights into the pathophysiology of renal cyst development in patients...
  27. ncbi request reprint Mutations of the Uromodulin gene in MCKD type 2 patients cluster in exon 4, which encodes three EGF-like domains
    Matthias T F Wolf
    Department of Pediatrics and Communicable Diseases, University of Michigan, Ann Arbor, Michigan 48109, USA
    Kidney Int 64:1580-7. 2003
    ..In contrast, recently, another group described mutations in the UMOD gene as responsible for MCKD2 and familial juvenile hyperuricemic nephropathy (FJHN)...
  28. ncbi request reprint Urinary clusterin levels in the rat correlate with the severity of tubular damage and may help to differentiate between glomerular and tubular injuries
    Sumi Hidaka
    Institute for Anatomy and Cell Biology I, University of Heidelberg, Germany
    Cell Tissue Res 310:289-96. 2002
    ..Urinary clusterin levels could therefore serve as a valuable marker for the severity of tubular damage. Furthermore, clusterin may also help to differentiate between tubular and glomerular forms of proteinuria...
  29. ncbi request reprint Altered expression pattern of polycystin-2 in acute and chronic renal tubular diseases
    Nicholas Obermüller
    Medical Research Center, Klinikum Mannheim, University of Heidelberg, Mannheim, Germany
    J Am Soc Nephrol 13:1855-64. 2002
    ..After ischemia, polycystin-2 may be upregulated by the injured cells to protect themselves. It is unlikely that polycystin-2 plays a role in cyst formation in the (cy/+) rat and in the (pcy/pcy) mouse...
  30. pmc The LIM-homeodomain transcription factor Lmx1b plays a crucial role in podocytes
    Claudia Rohr
    Institute for Anatomy and Cell Biology I, University of Heidelberg, Im Neuenheimer Feld 307, 69120 Heidelberg, Germany
    J Clin Invest 109:1073-82. 2002
    ....
  31. ncbi request reprint Polycystin-2 is an intracellular calcium release channel
    Peter Koulen
    Department of Pharmacology, Yale University School of Medicine, 333 Cedar Street, New Haven, Connecticut 06520, USA
    Nat Cell Biol 4:191-7. 2002
    ....