Evi S Lianidou

Summary

Affiliation: University of Athens
Country: Greece

Publications

  1. pmc Gene expression profiling and DNA methylation analyses of CTCs
    Evi S Lianidou
    Analysis of Circulating Tumor Cells Lab, Lab of Analytical Chemistry, Department of Chemistry, University of Athens, 15771, Greece Electronic address
    Mol Oncol 10:431-42. 2016
  2. doi request reprint The Role of CTCs as Tumor Biomarkers
    Evi S Lianidou
    Analysis of Circulating Tumor Cells Lab, Lab of Analytical Chemistry, Department of Chemistry, University of Athens, 15771, Athens, Greece
    Adv Exp Med Biol 867:341-67. 2015
  3. pmc Breast cancer metastasis suppressor-1 promoter methylation in primary breast tumors and corresponding circulating tumor cells
    Maria Chimonidou
    Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Athens 15771, Greece
    Mol Cancer Res 11:1248-57. 2013
  4. pmc Clinical challenges in the molecular characterization of circulating tumour cells in breast cancer
    E S Lianidou
    Analysis of Circulating Tumor Cells Lab, Lab of Analytical Chemistry, Department of Chemistry, University of Athens, 15771 Athens, Greece
    Br J Cancer 108:2426-32. 2013
  5. doi request reprint Molecular characterization of circulating tumor cells in breast cancer: challenges and promises for individualized cancer treatment
    Evi S Lianidou
    Analysis of Circulating Tumor Cells Lab, Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, 15771 Athens, Greece
    Cancer Metastasis Rev 31:663-71. 2012
  6. doi request reprint Molecular assays for the detection and characterization of CTCs
    Evi S Lianidou
    Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, 15771, Athens, Greece
    Recent Results Cancer Res 195:111-23. 2012
  7. pmc Hypoxia-inducible factor-1alpha and vascular endothelial growth factor expression in circulating tumor cells of breast cancer patients
    Galatea Kallergi
    Laboratory of Tumor Cell Biology, School of Medicine, University of Crete, Voutes, Heraklion, 71110, Greece
    Breast Cancer Res 11:R84. 2009
  8. pmc Gene expression profile of circulating tumor cells in breast cancer by RT-qPCR
    Areti Strati
    Department of Chemistry, University of Athens, University Campus, 15771 Athens, Greece
    BMC Cancer 11:422. 2011
  9. doi request reprint Circulating tumor cells as emerging tumor biomarkers in breast cancer
    Evi S Lianidou
    Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Athens, Greece
    Clin Chem Lab Med 49:1579-90. 2011
  10. doi request reprint Circulating tumor cells in breast cancer: detection systems, molecular characterization, and future challenges
    Evi S Lianidou
    Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Athens, Greece
    Clin Chem 57:1242-55. 2011

Collaborators

Detail Information

Publications37

  1. pmc Gene expression profiling and DNA methylation analyses of CTCs
    Evi S Lianidou
    Analysis of Circulating Tumor Cells Lab, Lab of Analytical Chemistry, Department of Chemistry, University of Athens, 15771, Greece Electronic address
    Mol Oncol 10:431-42. 2016
    ..This review is mainly focused on gene expression and methylation studies in CTCs in various types of cancer. ..
  2. doi request reprint The Role of CTCs as Tumor Biomarkers
    Evi S Lianidou
    Analysis of Circulating Tumor Cells Lab, Lab of Analytical Chemistry, Department of Chemistry, University of Athens, 15771, Athens, Greece
    Adv Exp Med Biol 867:341-67. 2015
    ..We also highlight the importance of performing comparison studies between these different methodologies and external quality control systems for establishing CTCs as tumor biomarkers in the routine clinical setting. ..
  3. pmc Breast cancer metastasis suppressor-1 promoter methylation in primary breast tumors and corresponding circulating tumor cells
    Maria Chimonidou
    Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Athens 15771, Greece
    Mol Cancer Res 11:1248-57. 2013
    ..In summary, these data define BRMS1 promoter methylation in primary breast tumors and associated CTCs...
  4. pmc Clinical challenges in the molecular characterization of circulating tumour cells in breast cancer
    E S Lianidou
    Analysis of Circulating Tumor Cells Lab, Lab of Analytical Chemistry, Department of Chemistry, University of Athens, 15771 Athens, Greece
    Br J Cancer 108:2426-32. 2013
    ..This review is mainly focused on the clinical potential of the molecular characterisation of CTC in BC. ..
  5. doi request reprint Molecular characterization of circulating tumor cells in breast cancer: challenges and promises for individualized cancer treatment
    Evi S Lianidou
    Analysis of Circulating Tumor Cells Lab, Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, 15771 Athens, Greece
    Cancer Metastasis Rev 31:663-71. 2012
    ..In this review, we briefly summarize recent studies on the molecular characterization of circulating tumor cells in breast cancer and discuss challenges and promises of CTCs for individualized cancer treatment...
  6. doi request reprint Molecular assays for the detection and characterization of CTCs
    Evi S Lianidou
    Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, 15771, Athens, Greece
    Recent Results Cancer Res 195:111-23. 2012
    ..Here, we present the main molecular detection technologies currently used for CTC analysis and molecular characterization...
  7. pmc Hypoxia-inducible factor-1alpha and vascular endothelial growth factor expression in circulating tumor cells of breast cancer patients
    Galatea Kallergi
    Laboratory of Tumor Cell Biology, School of Medicine, University of Crete, Voutes, Heraklion, 71110, Greece
    Breast Cancer Res 11:R84. 2009
    ..Since hypoxia-inducible factor-1alpha (HIF-1alpha) and vascular endothelial growth factor (VEGF) play an important role in angiogenesis and tumor progression, the purpose of the current study was to investigate their expression in CTCs...
  8. pmc Gene expression profile of circulating tumor cells in breast cancer by RT-qPCR
    Areti Strati
    Department of Chemistry, University of Athens, University Campus, 15771 Athens, Greece
    BMC Cancer 11:422. 2011
    ..Molecular characterization of CTCs is difficult to address since they are very rare and the amount of available sample is very limited...
  9. doi request reprint Circulating tumor cells as emerging tumor biomarkers in breast cancer
    Evi S Lianidou
    Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Athens, Greece
    Clin Chem Lab Med 49:1579-90. 2011
    ..This review has focused on the presentation of recent data showing that CTCs are emerging as novel tumor biomarkers for prognostic and predictive purposes in breast cancer...
  10. doi request reprint Circulating tumor cells in breast cancer: detection systems, molecular characterization, and future challenges
    Evi S Lianidou
    Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Athens, Greece
    Clin Chem 57:1242-55. 2011
    ..Circulating tumor cell (CTC) analysis is a promising new diagnostic field for estimating the risk for metastatic relapse and metastatic progression in patients with cancer...
  11. pmc What's new on circulating tumor cells? A meeting report
    Evi S Lianidou
    Department of Chemistry, University of Athens, 15771 Athens, Greece
    Breast Cancer Res 12:307. 2010
    ..A liquid biopsy approach that can be used for prognostic and predictive purposes was proposed for the analysis of CTCs...
  12. ncbi request reprint Detection of Mammaglobin A-mRNA-positive circulating tumor cells in peripheral blood of patients with operable breast cancer with nested RT-PCR
    Maria Ntoulia
    Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Greece
    Clin Biochem 39:879-87. 2006
    ....
  13. doi request reprint Methylation of cystatin M promoter is associated with unfavorable prognosis in operable breast cancer
    Magdalini Kioulafa
    Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Athens, Greece
    Int J Cancer 125:2887-92. 2009
    ..989-42.454; p=0.004). CST6 promoter methylation status in tumor cells seems to provide important prognostic information in operable breast cancer and merits to be further evaluated and validated in a larger cohort of patients...
  14. ncbi request reprint Effect of antineoplastic agents on the expression of human telomerase reverse transcriptase beta plus transcript in MCF-7 cells
    Tonia Spiropoulou
    Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, 15771 Athens, Greece
    Clin Biochem 37:299-304. 2004
    ..To evaluate the effect of antineoplastic agents on the expression of human telomerase reverse transcriptase (hTERT) splice variants in MCF-7 cells...
  15. ncbi request reprint A highly specific real-time RT-PCR method for the quantitative determination of CK-19 mRNA positive cells in peripheral blood of patients with operable breast cancer
    Aliki Stathopoulou
    Laboratory of Analytical Chemistry, University of Athens, Athens, Greece
    Int J Cancer 119:1654-9. 2006
    ....
  16. doi request reprint DNA methylation of tumor suppressor and metastasis suppressor genes in circulating tumor cells
    Maria Chimonidou
    Department of Chemistry, University of Athens, Greece
    Clin Chem 57:1169-77. 2011
    ..We show that tumor suppressor and metastasis suppressor genes are epigenetically silenced in CTCs isolated from peripheral blood of breast cancer patients...
  17. ncbi request reprint Quantitative real-time reverse transcription PCR study of the expression of vascular endothelial growth factor (VEGF) splice variants and VEGF receptors (VEGFR-1 and VEGFR-2) in non small cell lung cancer
    Eleni Zygalaki
    Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Athens 15771, Greece
    Clin Chem 53:1433-9. 2007
    ..We aimed to determine the expression pattern of VEGF splice variants in NSCLC and its correlation with the clinicopathological characteristics of tumors...
  18. ncbi request reprint HER-2 DNA quantification of paraffin-embedded breast carcinomas with LightCycler real-time PCR in comparison to immunohistochemistry and chromogenic in situ hybridization
    Maria Ntoulia
    Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Athens 15771, Greece
    Clin Biochem 39:942-6. 2006
    ..To compare the detection of HER-2 status by real-time PCR, on paraffin-embedded breast carcinomas, in respect to immunohistochemistry (IHC) and chromogenic in situ hybridization (CISH)...
  19. ncbi request reprint Development and applications of a real-time quantitative RT-PCR method (QRT-PCR) for BRCA1 mRNA
    Christos Kroupis
    Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Athens 15771, Greece
    Clin Biochem 38:50-7. 2005
    ..To develop a real-time quantitative RT-PCR method for BRCA1 mRNA and then use it for the study of BRCA1 gene expression in human MCF-7 breast cancer cells after their exposure to antineoplastic agents and gamma irradiation...
  20. ncbi request reprint Expression profile of total VEGF, VEGF splice variants and VEGF receptors in the myocardium and arterial vasculature of diabetic and non-diabetic patients with coronary artery disease
    Eleni Zygalaki
    Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, 15771, Athens, Greece
    Clin Biochem 41:82-7. 2008
    ..Vascular endothelial growth factor (VEGF) is a key regulator of angiogenesis and is implicated in the development of diabetic microvascular and macrovascular disease...
  21. ncbi request reprint Development of a highly sensitive RT-PCR luminometric hybridization assay for human telomerase reverse-transcriptase beta-plus transcript
    Katerina Angelopoulou
    Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Athens, 15772, Greece
    Anticancer Res 23:4821-9. 2003
    ..Alternate splicing of human telomerase reverse transcriptase (hTERT) has been shown to affect telomerase activity...
  22. doi request reprint Prognostic significance of RASSF1A promoter methylation in operable breast cancer
    Magdalini Kioulafa
    Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Athens, 15771, Greece
    Clin Biochem 42:970-5. 2009
    ..The aim of our study was to evaluate the prognostic significance of RASSF1A promoter methylation status in operable breast cancer...
  23. pmc PIK3CA hotspot mutation scanning by a novel and highly sensitive high-resolution small amplicon melting analysis method
    Panagiotis A Vorkas
    Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Athens, Greece
    J Mol Diagn 12:697-704. 2010
    ..The PIK3CA HRMA assay is highly sensitive, reliable, cost-effective, and easy-to-perform, and therefore can be used as a screening test in a high-throughput pharmacodiagnostic setting...
  24. doi request reprint Prognostic value of mature microRNA-21 and microRNA-205 overexpression in non-small cell lung cancer by quantitative real-time RT-PCR
    Athina Markou
    Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Athens, Greece
    Clin Chem 54:1696-704. 2008
    ..We evaluated the prognostic value of mature microRNA-21 (miR-21) and mature microRNA-205 (miR-205) overexpression in non-small cell lung cancer (NSCLC)...
  25. pmc A closed-tube methylation-sensitive high resolution melting assay (MS-HRMA) for the semi-quantitative determination of CST6 promoter methylation in clinical samples
    Lampros Dimitrakopoulos
    Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Athens, 15771, Greece
    BMC Cancer 12:486. 2012
    ..The aim of our study was to develop a Methylation-Sensitive High Resolution Melting Analysis (MS-HRMA) assay for the investigation of CST6 promoter methylation...
  26. doi request reprint SOX17 promoter methylation in circulating tumor cells and matched cell-free DNA isolated from plasma of patients with breast cancer
    Maria Chimonidou
    Analysis of Circulating Tumor Cells Laboratory, Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Athens, Greece
    Clin Chem 59:270-9. 2013
    ..We investigated whether SRY (sex determining region Y)-box 17 (SOX17) promoter methylation in CTCs was associated with the methylation pattern of this gene in matched cfDNA isolated from plasma of patients with breast cancer...
  27. ncbi request reprint Real-time RT-PCR quantification of human telomerase reverse transcriptase splice variants in tumor cell lines and non-small cell lung cancer
    Eleni Mavrogiannou
    Laboratory of Analytical Chemistry, University of Athens, Athens, Greece
    Clin Chem 53:53-61. 2007
    ....
  28. ncbi request reprint Real-time reverse transcription-PCR quantification of vascular endothelial growth factor splice variants
    Eleni Zygalaki
    Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Athens, Greece
    Clin Chem 51:1518-20. 2005
  29. doi request reprint Molecular characterization of circulating tumor cells in breast cancer by a liquid bead array hybridization assay
    Athina Markou
    Laboratory of Analytical Chemistry, University of Athens, and Medical Oncology Unit, Helena Venizelou Hospital, Athens, Greece
    Clin Chem 57:421-30. 2011
    ..We developed a multiplexed PCR-coupled liquid bead array to detect the expression of multiple genes in CTCs...
  30. ncbi request reprint Systemic hypertension augments, whereas insulin-dependent diabetes down-regulates, endothelin A receptor expression in the mammary artery in coronary artery disease patients
    Eleni Zygalaki
    Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Greece
    Cardiol J 16:348-54. 2009
    ..Endothelin (ET) A receptor antagonism causes decreased vasodilation in hypertensive coronary arteries and decreased effects on coronary artery compliance in diabetic patients...
  31. doi request reprint Asymmetric real-time PCR detection of BRCA1 5382insC mutation by melting curve analysis in the LightCycler
    Christos Kroupis
    Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Athens 15771, Greece
    Clin Chim Acta 390:141-4. 2008
    ..Our goal was to develop a novel, reliable and rapid method for its detection...
  32. doi request reprint Mutation scanning of exon 20 of the BRCA1 gene by high-resolution melting curve analysis
    Panagiotis A Vorkas
    Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Athens 15771, Greece
    Clin Biochem 43:178-85. 2010
    ..Our goal was to develop a novel, reliable and rapid genotyping/scanning method for mutation detection of the exon 20 of the BRCA1 gene, using high-resolution melting curve analysis...
  33. ncbi request reprint Presence of high-risk human papillomavirus sequences in breast cancer tissues and association with histopathological characteristics
    Christos Kroupis
    Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Athens 15771, Greece
    Clin Biochem 39:727-31. 2006
    ..To examine the presence of human papillomavirus (HPV) in breast cancer tissues...
  34. doi request reprint Quantification of circulating miRNAs in plasma: effect of preanalytical and analytical parameters on their isolation and stability
    Ioanna S Sourvinou
    Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Athens, Greece
    J Mol Diagn 15:827-34. 2013
    ..Experimental handling and the use of exogenous and endogenous controls for normalization are critical for the reliable quantification of circulating miRNA levels in plasma. ..
  35. ncbi request reprint Real-time quantification of CK-19 mRNA-positive cells in peripheral blood of breast cancer patients using the lightcycler system
    Aliki Stathopoulou
    Laboratory of Analytical Chemistry, University of Athens, Athens, Greece
    Clin Cancer Res 9:5145-51. 2003
    ....
  36. doi request reprint Circulating tumor cells as promising novel biomarkers in solid cancers
    Evi S Lianidou
    Analysis of Circulating Tumor Cells Laboratory, Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, Athens, Greece
    Crit Rev Clin Lab Sci 51:160-71. 2014
    ....
  37. doi request reprint Lung cancer epigenetics: emerging biomarkers
    Ioanna Balgkouranidou
    Laboratory of Analytical Chemistry, Department of Chemistry, University of Athens, 15771 Athens, Greece
    Biomark Med 7:49-58. 2013
    ..This process includes extensive validation in prospective clinical trials before they can be routinely used in a clinical setting. This review summarizes the evidence on epigenetic biomarkers for lung cancer, focusing on DNA methylation...