V Shkap

Summary

Affiliation: The Hebrew University
Country: Israel

Publications

  1. ncbi request reprint Seroprevalence of Babesia equi among horses in Israel using competitive inhibition ELISA and IFA assays
    V Shkap
    Department of Parasitology, Kimron Veterinary Institute, Bet Dagon, Israel
    Vet Parasitol 76:251-9. 1998
  2. ncbi request reprint Attenuated vaccines for tropical theileriosis, babesiosis and heartwater: the continuing necessity
    Varda Shkap
    Division of Parasitology, Kimron Veterinary Institute, Bet Dagan, Israel
    Trends Parasitol 23:420-6. 2007
  3. ncbi request reprint Low seroprevalence of antibodies to Neospora caninum in wild canids in Israel
    A Steinman
    Koret School of Veterinary Medicine, Faculty of Agricultural, Food and Environmental Sciences, The Hebrew University of Jerusalem, P O Box 12, Rehovot 76100, Israel
    Vet Parasitol 137:155-8. 2006
  4. ncbi request reprint Molecular and serological detection of A. centrale- and A. marginale-infected cattle grazing within an endemic area
    T Molad
    Division of Parasitology, Kimron Veterinary Institute, P O Box 12, Bet Dagan 50250, Israel
    Vet Microbiol 113:55-62. 2006
  5. ncbi request reprint Genetic diversity of anaplasma species major surface proteins and implications for anaplasmosis serodiagnosis and vaccine development
    Jose De La Fuente
    Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078 2007, USA
    Anim Health Res Rev 6:75-89. 2005
  6. ncbi request reprint Immunological relationship between Neospora caninum and Besnoitia besnoiti
    Varda Shkap
    Division of Parasitology, The Kimron Veterinary Institute, P O Box 12, 50250 Bet Dagan, Israel
    Vet Parasitol 106:35-43. 2002
  7. ncbi request reprint Vaccination of older Bos taurus bulls against bovine babesiosis
    Varda Shkap
    Division of Parasitology, Kimron Veterinary Institute, P O Box 12, Bet Dagan 50250, Israel
    Vet Parasitol 129:235-42. 2005
  8. ncbi request reprint Proteolytic enzyme activity and attenuation of virulence in Theileria annulata schizont-infected cells
    Varda Shkap
    Division of Parasitology, Kimron Veterinary Institute, P O Box 12, Bet Dagan 50250, Israel
    Vet Parasitol 115:247-55. 2003
  9. ncbi request reprint Detection of the Anaplasma centralevaccine strain and specific differentiation from Anaplasma marginale in vaccinated and infected cattle
    V Shkap
    Division of Parasitology, Kimron Veterinary Institute P O Box 12, Bet Dagan 50250, Israel
    Parasitol Res 88:546-52. 2002
  10. ncbi request reprint Culture-derived parasites in vaccination of cattle against tick-borne diseases
    V Shkap
    Kimron Veterinary Institute, P O Box 12, Bet Dagan, 50250, Israel
    Ann N Y Acad Sci 916:154-71. 2000

Collaborators

Detail Information

Publications46

  1. ncbi request reprint Seroprevalence of Babesia equi among horses in Israel using competitive inhibition ELISA and IFA assays
    V Shkap
    Department of Parasitology, Kimron Veterinary Institute, Bet Dagon, Israel
    Vet Parasitol 76:251-9. 1998
    ..No significant association was found between the mean age of horses and seroreactivity to B. equi...
  2. ncbi request reprint Attenuated vaccines for tropical theileriosis, babesiosis and heartwater: the continuing necessity
    Varda Shkap
    Division of Parasitology, Kimron Veterinary Institute, Bet Dagan, Israel
    Trends Parasitol 23:420-6. 2007
    ..As a result, there is a continuing necessity to use available live vaccines in tick and tick-borne disease-control strategies adapted to conditions prevailing in many parts of the world...
  3. ncbi request reprint Low seroprevalence of antibodies to Neospora caninum in wild canids in Israel
    A Steinman
    Koret School of Veterinary Medicine, Faculty of Agricultural, Food and Environmental Sciences, The Hebrew University of Jerusalem, P O Box 12, Rehovot 76100, Israel
    Vet Parasitol 137:155-8. 2006
    ....
  4. ncbi request reprint Molecular and serological detection of A. centrale- and A. marginale-infected cattle grazing within an endemic area
    T Molad
    Division of Parasitology, Kimron Veterinary Institute, P O Box 12, Bet Dagan 50250, Israel
    Vet Microbiol 113:55-62. 2006
    ..In the light of wide natural field infection with A. marginale, strong recommendations to continue the A. centrale vaccination program regime will continue until a new generation of non-blood-based vaccine will be developed...
  5. ncbi request reprint Genetic diversity of anaplasma species major surface proteins and implications for anaplasmosis serodiagnosis and vaccine development
    Jose De La Fuente
    Department of Veterinary Pathobiology, Center for Veterinary Health Sciences, Oklahoma State University, Stillwater, OK 74078 2007, USA
    Anim Health Res Rev 6:75-89. 2005
    ..Information concerning the genetic diversity of Anaplasma spp. MSPs will likely influence the development of serodiagnostic assays and vaccine strategies for the control of anaplasmosis...
  6. ncbi request reprint Immunological relationship between Neospora caninum and Besnoitia besnoiti
    Varda Shkap
    Division of Parasitology, The Kimron Veterinary Institute, P O Box 12, 50250 Bet Dagan, Israel
    Vet Parasitol 106:35-43. 2002
    ..caninum antigen was probed with B. besnoiti antiserum. Immunization and challenge experiments in the highly susceptible Mongolian gerbil (Meriones unguiculatus) showed essentially no cross-protection between N. caninum and B. besnoiti...
  7. ncbi request reprint Vaccination of older Bos taurus bulls against bovine babesiosis
    Varda Shkap
    Division of Parasitology, Kimron Veterinary Institute, P O Box 12, Bet Dagan 50250, Israel
    Vet Parasitol 129:235-42. 2005
    ....
  8. ncbi request reprint Proteolytic enzyme activity and attenuation of virulence in Theileria annulata schizont-infected cells
    Varda Shkap
    Division of Parasitology, Kimron Veterinary Institute, P O Box 12, Bet Dagan 50250, Israel
    Vet Parasitol 115:247-55. 2003
    ..excavatum sporozoites. For a strain to be used for vaccine production, it appears that animal inoculation still remains the most reliable method to assess the degree of attenuation and protection...
  9. ncbi request reprint Detection of the Anaplasma centralevaccine strain and specific differentiation from Anaplasma marginale in vaccinated and infected cattle
    V Shkap
    Division of Parasitology, Kimron Veterinary Institute P O Box 12, Bet Dagan 50250, Israel
    Parasitol Res 88:546-52. 2002
    ..The lack of severe A. marginaleoutbreaks in this region, despite ongoing transmission, is consistent with protection being provided by widespread vaccination with A. centrale...
  10. ncbi request reprint Culture-derived parasites in vaccination of cattle against tick-borne diseases
    V Shkap
    Kimron Veterinary Institute, P O Box 12, Bet Dagan, 50250, Israel
    Ann N Y Acad Sci 916:154-71. 2000
    ....
  11. doi request reprint Genetic diversity of major surface protein 1a of Anaplasma marginale in beef cattle
    T Molad
    Division of Parasitology, Kimron Veterinary Institute, Bet Dagan 50250, Israel
    Vet Microbiol 136:54-60. 2009
    ..Learning how this diversity is generated and identification of distinct A. marginale strains coupled with high sequence variation of MSP1a will aid in understanding Anaplasma transmission and disease development...
  12. ncbi request reprint Babesia bovis and B. bigemina: Persistence of infection in friesian cows following vaccination with live antibabesial vaccines
    E Pipano
    Division of Parasitology, The Kimron Veterinary Institute, Bet Dagan 50250, Israel
    Vet J 164:64-8. 2002
    ..It is concluded that in the absence of reinfection Friesian cattle may spontaneously eliminate B. bigemina and B. bovis infection after various periods following vaccination...
  13. doi request reprint Genetic diversity of Babesia bovis in virulent and attenuated strains
    M L Mazuz
    Division of Parasitology, Kimron Veterinary Institute, Bet Dagan, Israel
    Parasitology 139:317-23. 2012
    ..Despite high polymorphism found by sequence analyses, the patterns observed and the number of repeats, order, or motifs found could not discriminate between virulent field isolates and attenuated vaccine strains of the parasite...
  14. ncbi request reprint Isolation of Neospora caninum from dairy zero grazing cattle in Israel
    L Fish
    Division of Parasitology, Kimron Veterinary Institute, PO Box 12, Bet Dagan 50250, Israel
    Vet Parasitol 149:167-71. 2007
    ..The inoculated gerbils developed specific antibodies to N. caninum, with end-point serum dilution of 1:4096 in the IFA assay, whereas no neurological signs or deaths were seen during 4 months of observation...
  15. ncbi request reprint Differentiating between Besnoitia besnoiti from cattle and Sarcocystis hoarensis from rodents
    V Shkap
    Department of Parasitology, Kimron Veterinary Institute, Beit Dagan, Israel
    Parasitol Res 81:490-3. 1995
    ..B. besnoiti, therefore, can unambiguously be differentiated from S. hoarensis either by morphological or serological methods or on the basis of experimental comparisons of virulence in laboratory rodents...
  16. doi request reprint Identification of Anaplasma centrale major surface protein-2 pseudogenes
    T Molad
    Division of Parasitology, Kimron Veterinary Institute, P O Box 12, Bet Dagan 50250, Israel
    Vet Microbiol 143:277-83. 2010
    ..marginale. The knowledge gained in the present study on the mechanisms of antigenic variations in the vaccine strain of A. centrale is a further step in the development of a new generation vaccine against anaplasmosis...
  17. ncbi request reprint Bovine besnoitiosis: transfer of colostral antibodies with observations possibly relating to natural transmission of the infection
    V Shkap
    Kimron Veterinary Institute, Beit Dagan, Israel
    Onderstepoort J Vet Res 61:273-5. 1994
    ..Observations concerning passive transfer of antibodies from Besnoitia-infected dams to offspring, and transmission of the infection among infected and non-infected closely kept cows, are discussed...
  18. ncbi request reprint The route of immunization of gerbils with live Besnoitia besnoiti as a factor in protection against lethal challenge
    V Shkap
    Division of Parasitology, Kimron Veterinary Institute, Bet Dagan, Israel
    Vet Parasitol 51:27-33. 1993
    ..There was no statistical difference between the immunofluorescent antibody titres developed in groups vaccinated subcutaneously or intraperitoneally (P = 0.556)...
  19. ncbi request reprint Activity of a monoclonal antibody against Besnoitia besnoiti endozoites
    V Shkap
    Department of Parasitology, Kimron Veterinary Institute, Bet Dagan, Israel
    Vet Res 26:328-34. 1995
    ..In Western blots the specific anti-B besnoiti MoAb of the IgG1 subclass recognized a 70 kDa endozoite protein in the cytosolic and the insoluble membrane fraction of endozoites...
  20. ncbi request reprint Susceptibility of Psammomys obesus and Meriones tristrami to tachyzoites of Neospora caninum
    E Pipano
    Division of Parasitology, The Kimron Veterinary Institute, Bet Dagan, Israel
    J Parasitol 88:314-9. 2002
    ..Seven successive passage of tachyzoites were achieved in sand rats with doses of 10(5) parasites and in jirds with doses of 10(7) parasites. All surviving jirds became seroconverted and were immune to lethal challenge...
  21. doi request reprint Genetic polymorphism of Babesia bovis merozoite surface antigens-2 (MSA-2) isolates from bovine blood and Rhipicephalus annulatus ticks in Israel
    T Molad
    Division of Parasitology, Kimron Veterinary Institute, P O Box 12, Bet Dagan 50250, Israel Electronic address
    Vet Parasitol 205:20-7. 2014
    ..Thus, further research will be needed in order to understand the genetic diversity mechanisms of B. bovis, and the immunological responses of the infected animals...
  22. doi request reprint Comparative analysis of mitochondrial markers from four species of Rhipicephalus (Acari: Ixodidae)
    O Erster
    Division of Parasitology, Kimron Veterinary Institute, P O Box 12, Bet Dagan 50250, Israel Electronic address
    Vet Parasitol 198:364-70. 2013
    ..It is anticipated that with the accumulation of more information on additional species and markers, molecular identification will become a standard approach for tick classification, complementing morphological taxonomy...
  23. ncbi request reprint Molecular conservation of MSP4 and MSP5 in Anaplasma marginale and A. centrale vaccine strain
    T Molad
    Division of Parasitology, Kimron Veterinary Institute, P O Box 12, Bet Dagan 50250, Israel
    Vet Microbiol 100:55-64. 2004
    ..centrale and A. marginale. This conservation is consistent with the cross-protective immunity between A. marginale and A. centrale and supports the development of improved vaccines based upon common outer membrane proteins...
  24. ncbi request reprint Babesia bigemina: attenuation of an Uzbek isolate for immunization of cattle with live calf- or culture-derived parasites
    Varda Shkap
    Kimron Veterinary Institute, P O Box 12, Bet Dagan 50250, Israel
    Vet Parasitol 146:221-6. 2007
    ..bigemina, as reported from various geographical regions, the Central Asian strain was attenuated similarly to those that form the basis of the existing live B. bigemina vaccines in other parts of the world...
  25. doi request reprint Vaccination of cattle against B. bovis infection with live attenuated parasites and non-viable immunogens
    Lea Fish
    Division of Parasitology, Kimron Veterinary Institute, Bet Dagan 50250, Israel
    Vaccine 26:G29-33. 2008
    ..Upon challenge with virulent live parasites all immunized calves were solidly protected, but only partial protective immunity was acquired by rRAP1 immunization...
  26. doi request reprint The effect of a live Neospora caninum tachyzoite vaccine in naturally infected pregnant dairy cows
    M L Mazuz
    Division of Parasitology, Kimron Veterinary Institute, Bet Dagan, 50250, Israel Department of Microbiology and Molecular Genetics, The Kuvin Centre for the Study of Infectious and Tropical Diseases, The Hebrew University of Jerusalem, Jerusalem, Israel Electronic address
    Prev Vet Med 120:232-5. 2015
    ..Overall, this field trial suggests that vaccination with live N. caninum tachyzoites should be considered as an effective measure to reduce abortions caused by neosporosis in naturally infected cows. ..
  27. ncbi request reprint An enzyme-linked immunosorbent assay for antibodies to Hepatozoon canis
    L Gonen
    School of Veterinary Medicine, Hebrew University, P O Box 12, Rehovot 76100, Israel
    Vet Parasitol 122:131-9. 2004
    ..The ELISA will be valuable for serological evaluation of dogs suspected of exposure to H. canis and for epidemiological studies...
  28. doi request reprint First detection of Ixodes ricinus on beef cattle in Israel
    O Erster
    Division of Parasitology, Kimron Veterinary Institute, Bet Dagan 50250, Israel
    Vet Parasitol 191:394-9. 2013
    ..These findings demonstrated for the first time the presence in Israel of the tick species I. ricinus - with results confirmed by a combination of morphological examination and molecular analyses...
  29. ncbi request reprint Theileria annulata: in vitro cultivation of schizont-infected bovine lymphocytes
    V Shkap
    Department of Parasitology, Kimron Veterinary Institute, Bet Dagan, Israel
    Vet Parasitol 65:11-20. 1996
    ..In Roller bottles, with the same type and volume of medium and cultivation for 7 days without medium change, best yield resulted from seeding with the highest inoculum size of 4 x 10(7) cells per vessel...
  30. ncbi request reprint Besnoitia besnoiti: quantitative in vitro studies
    V Shkap
    Division of Parasitology, Kimron Veterinary Institute, Bet Dagan, Israel
    Vet Parasitol 39:207-13. 1991
    ....
  31. ncbi request reprint Antibody response to Hepatozoon canis in experimentally infected dogs
    G Baneth
    School of Veterinary Medicine, Hebrew University of Jerusalem, Rehovot, Israel
    Vet Parasitol 74:299-305. 1998
    ..Detection of IgG titres would be beneficial for the diagnosis of progressive infections with undetectable parasitemia, for seroprevalence studies, and as an adjunct to IgM titres in early infections...
  32. ncbi request reprint Monozoic cysts of Hepatozoon canis
    Gad Baneth
    School of Veterinary Medicine, Hebrew University of Jerusalem, P O Box 12, Rehovot 76100, Israel
    J Parasitol 89:379-81. 2003
    ..The H. canis cyst stage differs in size and morphology from the large cysts of H. americanum, the second Hepatozoon species known to infect dogs...
  33. ncbi request reprint Canine hepatozoonosis: two disease syndromes caused by separate Hepatozoon spp
    Gad Baneth
    School of Veterinary Medicine, Hebrew University, P O Box 12, Rehovot 76100, Israel
    Trends Parasitol 19:27-31. 2003
  34. ncbi request reprint Immunity against Boophilus annulatus induced by the Bm86 (Tick-GARD) vaccine
    Eugene Pipano
    Veterinary Services and Animal Health, Kimron Veterinary Institute, Ministry of Agriculture and Rural Development, POB 12, 50250 Bet Dagan, Israel
    Exp Appl Acarol 29:141-9. 2003
    ..bigemina-infected females did not. Since B. bigemina is transmitted exclusively by nymphal stages of Bo. annulatus these results support the observation that immunity induced by Bm86 affects the larval stage of this tick...
  35. pmc Infection with a proposed new subspecies of Babesia canis, Babesia canis subsp. presentii, in domestic cats
    Gad Baneth
    School of Veterinary Medicine, Hebrew University, Rehovot 76100, Israel
    J Clin Microbiol 42:99-105. 2004
    ..canis present in dogs, we propose to identify the novel feline genotype of B. canis described in the present study as a new subspecies, B. canis subsp. presentii...
  36. ncbi request reprint Seroprevalence of Neospora spp. among asymptomatic horses, aborted mares and horses demonstrating neurological signs in Israel
    Eitan B Kligler
    Koret School of Veterinary Medicine, Faculty of Agricultural, Food and Environmental Quality Sciences, The Hebrew University of Jeruslem, P O Box 12, Rehovot 76100, Israel
    Vet Parasitol 148:109-13. 2007
    ..antibodies performed on horses from the Middle East and the first to report significant difference in seropositivity between asymptomatic horses and horses exhibiting neurological signs...
  37. ncbi request reprint Life cycle of Hepatozoon canis (Apicomplexa: Adeleorina: Hepatozoidae) in the tick Rhipicephalus sanguineus and domestic dog (Canis familiaris)
    Gad Baneth
    School of Veterinary Medicine, Hebrew University of Jerusalem, PO Box 12, Rehovot 76100, Israel
    J Parasitol 93:283-99. 2007
    ..Skeletal pain and recumbency were manifested in 2 dogs. This study further elucidates the life cycle of H. canis and provides a sequential morphologic description of H. canis merogony, gamontogony, and sporogony...
  38. doi request reprint Experimental transmission of field Anaplasma marginale and the A. centrale vaccine strain by Hyalomma excavatum, Rhipicephalus sanguineus and Rhipicephalus (Boophilus) annulatus ticks
    Varda Shkap
    Division of Parasitology, Kimron Veterinary Institute, Bet Dagan 50250, Israel
    Vet Microbiol 134:254-60. 2009
    ..sanguineus, no transmission to susceptible cattle occurred during 3 months of observation, and anaplasmosis was not induced in splenectomized calves that were subinoculated with blood from calves on which R. sanguineus had fed...
  39. pmc Expression of major surface protein 2 variants with conserved T-cell epitopes in Anaplasma centrale vaccinates
    Varda Shkap
    Department of Parasitology, Kimron Veterinary Institute, Bet Dagan, Israel
    Infect Immun 70:642-8. 2002
    ..centrale vaccine efficacy...
  40. doi request reprint Concomitant infection of cattle with the vaccine strain Anaplasma marginale ss centrale and field strains of A. marginale
    Varda Shkap
    Division of Parasitology, Kimron Veterinary Institute, Bet Dagan, Israel
    Vet Microbiol 130:277-84. 2008
    ..The present results confirmed our previous findings that cattle grazing in an anaplasmosis-endemic field were subject to concomitant infection with both the vaccine A. centrale and the field A. marginale strains...
  41. pmc Expression of Anaplasma marginale major surface protein 2 operon-associated proteins during mammalian and arthropod infection
    CHRISTIANE V LOHR
    Program in Vector Borne Diseases, Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, Washington 99164 7040, USA
    Infect Immun 70:6005-12. 2002
    ....
  42. ncbi request reprint Pan-Mediterranean comparison for the molecular detection of Theileria annulata
    Olivier A E Sparagano
    School of Agriculture, Food and Rural Development, University of Newcastle, Newcastle upon Tyne NE1 7RU, UK
    Ann N Y Acad Sci 969:73-7. 2002
    ..4 to 91.3%. The overall mismatch between laboratories was around 30% by whatever technique used. This paper describes the methodological parameters that could explain the variation of results...
  43. ncbi request reprint Phylogenetic analysis of the erythrocytic Anaplasma species based on 16S rDNA and GroEL (HSP60) sequences of A. marginale, A. centrale, and A. ovis and the specific detection of A. centrale vaccine strain
    Ala E Lew
    Department of Primary Industries, Agency for Food and Fibre Sciences, c o Animal Research Institute, 665 Fairfield Rd, Locked Mail Bag No 4, Yeerongpilly, Moorooka, Queensland 4105, Australia
    Vet Microbiol 92:145-60. 2003
    ..centrale vaccine (Theiler, 1911) specifically. This study confirms the suitability of 16S rDNA sequences to define genera and demonstrates the usefulness of GroEL sequences for defining species of erythrocytic Anaplasma...
  44. pmc Identification of midgut and salivary glands as specific and distinct barriers to efficient tick-borne transmission of Anaplasma marginale
    Massaro W Ueti
    Department of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA 99164 7040, USA
    Infect Immun 75:2959-64. 2007
    ..These results establish that there are at least two specific barriers to efficient tick-borne transmission, the midgut and salivary glands, and highlight the complexity of the pathogen-tick interaction...
  45. pmc Emergence of distinct genotypes of Cryptosporidium parvum in structured host populations
    Sultan Tanriverdi
    Division of Infectious Diseases, Tufts Cummings School of Veterinary Medicine, 200 Westboro Road, North Grafton, MA 01536, USA
    Appl Environ Microbiol 72:2507-13. 2006
    ..This may explain the frequent detection of host-specific genotypes with unknown taxonomic status in surface water and the existence of geographically restricted C. hominis genotypes in humans...
  46. pmc Bovine immune response to inoculation with Neospora caninum surface antigen SRS2 lipopeptides mimics immune response to infection with live parasites
    Timothy V Baszler
    Department of Veterinary Microbiology and Pathology, Bustad Hall, Washington State University, Pullman, WA 99164 7040, USA
    Clin Vaccine Immunol 15:659-67. 2008
    ..The findings support the investigation of NcSRS2 immunogens for protection against N. caninum-induced fetal infection and abortion in cattle...