Tiziana A L Brevini

Summary

Affiliation: University of Milan
Country: Italy

Publications

  1. ncbi request reprint Impact of endocrine disrupters on ovarian function and embryonic development
    F Gandolfi
    Instituto di Anatomia degli Animali Domestici, Via Celoria, 10 20133 Milano, Italy
    Domest Anim Endocrinol 23:189-201. 2002
  2. ncbi request reprint Porcine embryonic stem cells: Facts, challenges and hopes
    T A L Brevini
    Biomedical Embryology Unit, Department of Animal Science, Centre for Stem Cell Research, University of Milan, Via Celoria 10, Milan, Italy
    Theriogenology 68:S206-13. 2007
  3. pmc The quest for an effective and safe personalized cell therapy using epigenetic tools
    T A L Brevini
    Laboratory of Biomedical Embryology, UniStem, Universita degli Studi di Milano, Via Celoria 10, 20133 Milan, Italy
    Clin Epigenetics 8:119. 2016
  4. doi request reprint Use of a Super-hydrophobic Microbioreactor to Generate and Boost Pancreatic Mini-organoids
    Tiziana A L Brevini
    Laboratory of Biomedical Embryology, Centre for Stem Cell Research, Universita degli Studi di Milano, Milan, 20133, Italy
    Methods Mol Biol . 2017
  5. pmc Epigenetic Conversion as a Safe and Simple Method to Obtain Insulin-secreting Cells from Adult Skin Fibroblasts
    Tiziana A L Brevini
    Laboratory of Biomedical Embryology, UniStem, Centre for Stem Cell Research, Universita degli Studi di Milano
    J Vis Exp . 2016
  6. doi request reprint Morphological and molecular changes of human granulosa cells exposed to 5-azacytidine and addressed toward muscular differentiation
    Tiziana A L Brevini
    Laboratory of Biomedical Embryology, Centre for Stem Cell Research, University of Milan, Via Celoria 10, 20133, Milan, Italy
    Stem Cell Rev 10:633-42. 2014
  7. doi request reprint Centrosome amplification and chromosomal instability in human and animal parthenogenetic cell lines
    Tiziana A L Brevini
    Laboratory of Biomedical Embryology, Centre for Stem Cell Research UniStem, Universita degli Studi di Milano, Via Celoria 10, 20133 Milan, Italy
    Stem Cell Rev 8:1076-87. 2012
  8. doi request reprint Pluripotency network in porcine embryos and derived cell lines
    Tal Brevini
    Laboratory of Biomedical Embryology, Centre for Stem Cell Research, Universita degli Studi di Milano, Milan, Italy
    Reprod Domest Anim 47:86-91. 2012
  9. doi request reprint Parthenogenesis in non-rodent species: developmental competence and differentiation plasticity
    T A L Brevini
    UniStem, Centre for Stem Cell Research, Laboratory of Biomedical Embryology, Universita degli Studi di Milano, Italy
    Theriogenology 77:766-72. 2012
  10. ncbi request reprint Somatostatin up-regulates topoisomerase II alpha expression and affects LNCaP cell cycle
    T A Brevini-Gandolfi
    Centre for Endocrinological Oncology, Department of Endocrinology, University of Milan, Milan, Italy
    Mol Cell Endocrinol 176:103-10. 2001

Collaborators

Detail Information

Publications47

  1. ncbi request reprint Impact of endocrine disrupters on ovarian function and embryonic development
    F Gandolfi
    Instituto di Anatomia degli Animali Domestici, Via Celoria, 10 20133 Milano, Italy
    Domest Anim Endocrinol 23:189-201. 2002
  2. ncbi request reprint Porcine embryonic stem cells: Facts, challenges and hopes
    T A L Brevini
    Biomedical Embryology Unit, Department of Animal Science, Centre for Stem Cell Research, University of Milan, Via Celoria 10, Milan, Italy
    Theriogenology 68:S206-13. 2007
    ..The identification of pluripotency-related molecular markers in the pig will also be examined. Finally, the ability to respond to specifically formulated medium with spontaneous as well as induced differentiation will be assessed...
  3. pmc The quest for an effective and safe personalized cell therapy using epigenetic tools
    T A L Brevini
    Laboratory of Biomedical Embryology, UniStem, Universita degli Studi di Milano, Via Celoria 10, 20133 Milan, Italy
    Clin Epigenetics 8:119. 2016
    ..We also provide prospective views of the possibility to directly convert one cell type into another, in a safe and robust way, for cell-based clinical trials and regenerative medicine...
  4. doi request reprint Use of a Super-hydrophobic Microbioreactor to Generate and Boost Pancreatic Mini-organoids
    Tiziana A L Brevini
    Laboratory of Biomedical Embryology, Centre for Stem Cell Research, Universita degli Studi di Milano, Milan, 20133, Italy
    Methods Mol Biol . 2017
    ..It also makes it possible to scale down experiments and work in smaller volumes and is therefore amenable for higher throughput applications...
  5. pmc Epigenetic Conversion as a Safe and Simple Method to Obtain Insulin-secreting Cells from Adult Skin Fibroblasts
    Tiziana A L Brevini
    Laboratory of Biomedical Embryology, UniStem, Centre for Stem Cell Research, Universita degli Studi di Milano
    J Vis Exp . 2016
    ..Since the protocol does not involve the use of any gene transfection, it is free of viral vectors and does not involve a stable pluripotent state, it is highly promising for translational medicine applications. ..
  6. doi request reprint Morphological and molecular changes of human granulosa cells exposed to 5-azacytidine and addressed toward muscular differentiation
    Tiziana A L Brevini
    Laboratory of Biomedical Embryology, Centre for Stem Cell Research, University of Milan, Via Celoria 10, 20133, Milan, Italy
    Stem Cell Rev 10:633-42. 2014
    ....
  7. doi request reprint Centrosome amplification and chromosomal instability in human and animal parthenogenetic cell lines
    Tiziana A L Brevini
    Laboratory of Biomedical Embryology, Centre for Stem Cell Research UniStem, Universita degli Studi di Milano, Via Celoria 10, 20133 Milan, Italy
    Stem Cell Rev 8:1076-87. 2012
    ....
  8. doi request reprint Pluripotency network in porcine embryos and derived cell lines
    Tal Brevini
    Laboratory of Biomedical Embryology, Centre for Stem Cell Research, Universita degli Studi di Milano, Milan, Italy
    Reprod Domest Anim 47:86-91. 2012
    ..Finally, we will review data presently available on functional key pluripotency-maintaining pathways in farm animals...
  9. doi request reprint Parthenogenesis in non-rodent species: developmental competence and differentiation plasticity
    T A L Brevini
    UniStem, Centre for Stem Cell Research, Laboratory of Biomedical Embryology, Universita degli Studi di Milano, Italy
    Theriogenology 77:766-72. 2012
    ..However data obtained with parthenogenetic cells indicate the presence of an intrinsic deregulation of the mechanisms controlling proliferation vs. differentiation and suggest their uni-parental origin as a possible cause...
  10. ncbi request reprint Somatostatin up-regulates topoisomerase II alpha expression and affects LNCaP cell cycle
    T A Brevini-Gandolfi
    Centre for Endocrinological Oncology, Department of Endocrinology, University of Milan, Milan, Italy
    Mol Cell Endocrinol 176:103-10. 2001
    ..The hormone was shown to exert a perturbing effect on both parameters considered, possibly related to its inhibitory action on LNCaP cell replication...
  11. ncbi request reprint Parthenogenetic cell lines: an unstable equilibrium between pluripotency and malignant transformation
    Tiziana A L Brevini
    Laboratory of Biomedical Embryology UniStem, Center for Stem Cell Research, Universita degli Studi di Milano, Facolta di Medicina Veterinaria, Via Celoria 10, Milan, Italy
    Curr Pharm Biotechnol 12:206-12. 2011
    ....
  12. doi request reprint No shortcuts to pig embryonic stem cells
    T A L Brevini
    Universita degli Studi di Milano, Laboratory of Biomedical Embryology, Anatomy and Histology Unit, Milano, Italy
    Theriogenology 74:544-50. 2010
    ..We feel that the understanding of these subtle but meaningful diversities may provide beneficial information about the isolation of genuine porcine embryonic stem cells...
  13. doi request reprint Culture conditions and signalling networks promoting the establishment of cell lines from parthenogenetic and biparental pig embryos
    Tiziana A L Brevini
    UniStem, Laboratory of Biomedical Embryology, Universita degli Studi di Milano, Milan, Italy
    Stem Cell Rev 6:484-95. 2010
    ....
  14. doi request reprint Cell lines derived from human parthenogenetic embryos can display aberrant centriole distribution and altered expression levels of mitotic spindle check-point transcripts
    Tiziana A L Brevini
    Laboratory of Biomedical Embryology, Centre for Stem Cell Research, University of Milan, 20133 Milan, Italy
    Stem Cell Rev 5:340-52. 2009
    ....
  15. doi request reprint Parthenogenesis as an approach to pluripotency: advantages and limitations involved
    Tiziana A L Brevini
    Laboratory of Biomedical Embryology, Center for Stem Cell Research, University of Milan, 20133, Milan, Italy
    Stem Cell Rev 4:127-35. 2008
    ....
  16. doi request reprint Parthenotes as a source of embryonic stem cells
    T A L Brevini
    Laboratory of Biomedical Embryology, Centre for Stem Cell Research, University of Milan, Milan, Italy
    Cell Prolif 41:20-30. 2008
    ....
  17. ncbi request reprint Temporal and spatial control of gene expression in early embryos of farm animals
    Tiziana A L Brevini
    Department of Anatomy of Domestic Animals, School of Veterinary Medicine, University of Milan, Italy
    Reprod Fertil Dev 19:35-42. 2007
    ..This brief overview will provide some suggestions on how these different mechanisms may be integrated and cooperate to ensure the correct initiation of embryonic development...
  18. ncbi request reprint Exposure of pig oocytes to PCBs during in vitro maturation: effects on developmental competence, cytoplasmic remodelling and communications with cumulus cells
    T A L Brevini
    Istituto di Anatomia degli Animali Domestici, Via Celoria 10, 20133 Milan, Italy
    Eur J Histochem 48:347-56. 2004
    ..These data indicated that accumulation of PCBs in the pig organism may have a detrimental effect on the reproductive efficiency in this species...
  19. ncbi request reprint Effects of endocrine disruptors on developmental and reproductive functions
    Tiziana A L Brevini
    Department of Anatomy of Domestic Animals, School of Veterinary Medicine, University of Milano, Italy
    Curr Drug Targets Immune Endocr Metabol Disord 5:1-10. 2005
    ..The detrimental effects of these compounds, and of EDs more in general, on health and reproduction will be discussed, presenting experimental data aimed at understanding the molecular mechanisms involved in their action...
  20. ncbi request reprint Cytoplasmic remodelling and the acquisition of developmental competence in pig oocytes
    T A L Brevini
    Department of Anatomy of Domestic Animals, School of Veterinary Medicine, University of Milan, Via Celoria 10, 20133 Milan, Italy
    Anim Reprod Sci 98:23-38. 2007
    ..We present recent experimental evidence, obtained in our laboratory, on the pig orthologues for kinesin KIF5B and Staufen, describe their expression patterns and discuss their possible role in oocyte maturation...
  21. ncbi request reprint Expression pattern of the maternal factor zygote arrest 1 (Zar1) in bovine tissues, oocytes, and embryos
    Tiziana A L Brevini
    Department of Anatomy of Domestic Animals, University of Milan, Milan, Italy
    Mol Reprod Dev 69:375-80. 2004
    ..Moreover, the identification of Zar1 transcription at the 4-cell stage represents the first characterization of one of the genes expressed in cattle embryos before the major onset of embryonic transcription...
  22. ncbi request reprint Evolution of mRNA polyadenylation between oocyte maturation and first embryonic cleavage in cattle and its relation with developmental competence
    T A L Brevini
    Department of Anatomy of Domestic Animals, University of Milan, Via Celoria, 10 20133 Milano, Italy
    Mol Reprod Dev 63:510-7. 2002
    ..Defective developmental competence is accompanied by abnormal polyadenylation levels of specific maternal mRNAs with synchrony between polyadenylation and cleavage emerging as an apparently important factor...
  23. ncbi request reprint The maternal legacy to the embryo: cytoplasmic components and their effects on early development
    T A Gandolfi
    Department of Endocrinology, University of Milan, Italy
    Theriogenology 55:1255-76. 2001
    ..Because most data are available from lower species these are presented together with appropriate reference to the mammalian oocyte when data are known, or when important differences have been described...
  24. doi request reprint Direct comparative analysis of conventional and directional freezing for the cryopreservation of whole ovaries
    Sara Maffei
    Laboratory of Biomedical Embryology, Universita degli Studi di Milano, Milan, Italy
    Fertil Steril 100:1122-31. 2013
    ..To compare conventional slow equilibrium cooling and directional freezing for cryopreservation of whole ovaries...
  25. doi request reprint Developmental potential of human oocytes after slow freezing or vitrification: a randomized in vitro study based on parthenogenesis
    Alessio Paffoni
    Department of Obstetrics, Gynaecology, and Neonatology, Infertility Unit, Fondazione Ospedale Maggiore Policlinico, Mangiagalli e Regina Elena, Milan, Italy
    Reprod Sci 15:1027-33. 2008
    ..8 + 1.9 vs. 5.8 + 1.7). In conclusion, parthenogenesis highlights a reduced potential of vitrified oocytes to cleave on day 3 compared with oocytes from slow-freezing...
  26. doi request reprint Effects of pre-mating nutrition on mRNA levels of developmentally relevant genes in sheep oocytes and granulosa cells
    Laura F Pisani
    Laboratory of Biomedical Embryology, Department of Animal Science, University of Milan, Via Celoria 10, 20133 Milano, Italy
    Reproduction 136:303-12. 2008
    ....
  27. ncbi request reprint Aroclor-1254 affects mRNA polyadenylation, translational activation, cell morphology, and DNA integrity of rat primary prostate cells
    Fabiana Cillo
    Laboratory of Biomedical Embriology, School of Veterinary Medicine, UniStem, University of Milan, Milan, Italy
    Endocr Relat Cancer 14:257-66. 2007
    ....
  28. ncbi request reprint Derivation and characterization of pluripotent cell lines from pig embryos of different origins
    Tiziana A L Brevini
    Department of Anatomy of Domestic Animals, Centre for Stem Cell Research, University of Milan, Via Celoria, 10, 20133 Milano, Italy
    Theriogenology 67:54-63. 2007
    ....
  29. pmc Procedure for rapid oocyte selection based on quantitative analysis of cumulus cell gene expression
    Stefania Ferrari
    Servizi Sforza srl, Medicina della Riproduzione, Milan, Italy
    J Assist Reprod Genet 27:429-34. 2010
    ..To develop a procedure for the analysis of gene expression in cumulus cells during the interval between ovum pick up and insemination to select the best oocytes for fertilization...
  30. ncbi request reprint Association between human oocyte developmental competence and expression levels of some cumulus genes
    Fabiana Cillo
    Laboratory of Biomedical Embryology, Centre for Stem Cells Research, University of Milan, Via Celoria 10, 20133 Milan, Italy
    Reproduction 134:645-50. 2007
    ....
  31. doi request reprint Characterization of the constitutive pig ovary heat shock chaperone machinery and its response to acute thermal stress or to seasonal variations
    Georgia Pennarossa
    Laboratory of Biomedical Embryology, Department of Health, Animal Science and Food Safety, Universita degli Studi di Milano, Milan, Italy
    Biol Reprod 87:119. 2012
    ....
  32. ncbi request reprint Efficiency of equilibrium cooling and vitrification procedures for the cryopreservation of ovarian tissue: comparative analysis between human and animal models
    Fulvio Gandolfi
    Department of Anatomy of Domestic Animals, University of Milan, Milan, Italy
    Fertil Steril 85:1150-6. 2006
    ..To compare the efficiency of equilibrium cooling and vitrification for cryopreservation of human ovarian tissue and to determine the best experimental model for developing new protocols...
  33. ncbi request reprint Role of adenosine triphosphate, active mitochondria, and microtubules in the acquisition of developmental competence of parthenogenetically activated pig oocytes
    Tiziana A L Brevini
    Department of Anatomy of Domestic Animals, Faculty of Veterinary Medicine, University of Milan, 10 20133 Milano, Italy
    Biol Reprod 72:1218-23. 2005
    ..This can be independent from the formation of the microtubule machinery required for the completion of chromosome disjunctions and does not affect the overall ATP content...
  34. ncbi request reprint In vitro development of human oocytes after parthenogenetic activation or intracytoplasmic sperm injection
    Alessio Paffoni
    Department of Obstetrics, Gynecology, and Neonatology, Ospedale Maggiore Policlinico, Mangiagalli and Regina Elena, Milan, Italy
    Fertil Steril 87:77-82. 2007
    ..To compare directly in vitro developmental competence between parthenogenetically activated and intracytoplasmic sperm injection (ICSI)-fertilized oocytes...
  35. pmc 5-azacytidine affects TET2 and histone transcription and reshapes morphology of human skin fibroblasts
    Elena F M Manzoni
    Laboratory of Biomedical Embryology, Centre for Stem Cell Research Università degli Studi di Milano, Milan, 20133, Italy
    Sci Rep 6:37017. 2016
    ..Our findings indicate that 5-aza-CR induced somatic cell transition to a higher plasticity state is activated by multiple regulations that accompany the demethylating effect exerted by the modifier...
  36. pmc Brief demethylation step allows the conversion of adult human skin fibroblasts into insulin-secreting cells
    Georgia Pennarossa
    Laboratory of Biomedical Embryology, UniStem, Center for Stem Cell Research, Universita degli Studi di Milano, Italy
    Proc Natl Acad Sci U S A 110:8948-53. 2013
    ..This work shows that it is possible to convert adult fibroblasts into insulin-secreting cells, avoiding both a stable pluripotent stage and any transgenic modification...
  37. doi request reprint Intercellular bridges are essential for human parthenogenetic cell survival
    Georgia Pennarossa
    Laboratory of Biomedical Embryology, UniStem, Center for Stem Cell Research, Department of Health, Animal Science and Food Safety, Universita degli Studi di Milano, Milan, Italy
    Mech Dev 136:30-9. 2015
    ....
  38. ncbi request reprint Cellular and molecular mechanisms mediating the effect of polychlorinated biphenyls on oocyte in vitro maturation
    Paola Pocar
    Department of Anatomy of Domestic Animals, University of Milano, 20133 Milano, Italy
    Reprod Toxicol 22:242-9. 2006
    ....
  39. doi request reprint Erase and Rewind: Epigenetic Conversion of Cell Fate
    Georgia Pennarossa
    Laboratory of Biomedical Embryology, UniStem, Universita degli Studi di Milano, Via Celoria 10, 20133, Milan, Italy
    Stem Cell Rev 12:163-70. 2016
    ..This approach is simple and direct and may represent a very promising tool for the regenerative medicine of several and diverse degenerative diseases. ..
  40. doi request reprint RFD Award Lecture 2009. In vitro maturation of farm animal oocytes: a useful tool for investigating the mechanisms leading to full-term development
    Fulvio Gandolfi
    Laboratory of Biomedical Embryology, Department of Animal Sciences, Universita degli Studi di Milano, Via Celoria, 10 20133, Milano, Italy
    Reprod Fertil Dev 22:495-507. 2010
    ..The amount of knowledge is far from complete and many laboratories are actively working to further expand it. In this review we will highlight the aspects of the ongoing research in which we have been actively involved...
  41. doi request reprint A putative protein structurally related to zygote arrest 1 (Zar1), Zar1-like, is encoded by a novel gene conserved in the vertebrate lineage
    Lorenzo Sangiorgio
    Department of Animal Pathology, Hygiene and Public Veterinary Health, Section of Biochemistry and Physiology, Faculty of Veterinary Medicine, University of Milan, Via Celoria 10, 20133 Milan, Italy
    Comp Biochem Physiol B Biochem Mol Biol 150:233-9. 2008
    ..In adult tissues Zar1-like transcript expression appears to be less restricted than Zar1, nevertheless, at least in bovine, both mRNAs are co-expressed in gonads, raising the question of a possible functional link...
  42. ncbi request reprint Effects of endocrine disrupters on the oocytes and embryos of farm animals
    T A L Brevini
    Department of Anatomy of Domestic Animals, University of Milan, Milan, Italy
    Reprod Domest Anim 40:291-9. 2005
    ..In particular the role of arylhydrocarbon receptor, maternal mRNA stability and cytoplasmic remodelling during oocyte maturation will be discussed in some details...
  43. doi request reprint Simple and Quick Method to Obtain a Decellularized, Functional Liver Bioscaffold
    Matteo Ghiringhelli
    Department of Health, Animal Science and Food Safety, Universita degli Studi di Milano, Milan, Italy
    Methods Mol Biol . 2017
    ..The procedure is fast, simple, and cheap and warrants an optimal organoid functionality that may find applications in both toxicological and transplantation studies...
  44. doi request reprint Newborn pig ovarian tissue xenografted into Severe Combined Immunodeficient (SCID) mice acquires limited responsiveness to gonadotropins
    L F Pisani
    Department of Animal Science, Laboratory of Biomedical Embryology, Universita degli Studi di Milano, 20133 Milano, Italy
    Theriogenology 74:557-62. 2010
    ..Therefore, development of gonadotropin responsiveness in ovarian fragments xenotransplanted in SCID mice was delayed compared to what occurs in the unaltered animal, and there was minimal response to exogenous gonadotropins...
  45. doi request reprint Recent progress in embryonic stem cell research and its application in domestic species
    T A L Brevini
    Department of Animal Science, Centre for Embryonic Stem Cell Research, Laboratory of Biomedical Embryology, University of Milan, Italy
    Reprod Domest Anim 43:193-9. 2008
    ..But, a real break through could arrive from the multipotent germ-line stem cells, virtually equivalent to ESC, derived from newborn and adult mouse testis...
  46. ncbi request reprint Cellular and molecular mechanisms regulating oocyte quality and the relevance for farm animal reproductive efficiency
    F Gandolfi
    Department of Anatomy of Domestic Animals, University of Milan, Via Celoria 10, 20133 Milan, Italy
    Rev Sci Tech 24:413-23. 2005
    ..Surprising new findings, such as ovarian stem cells that can replenish the follicle population or long term embryonic stem cell lines that can differentiate into oocytes, are rapidly changing our expectations...
  47. ncbi request reprint The impact of endocrine disruptors on oocyte competence
    P Pocar
    Department of Anatomy and Cell Biology, Martin Luther University, Halle Saale Germany
    Reproduction 125:313-25. 2003
    ..However, as most of the data available still derive from experiments performed on laboratory species or in vitro models, great care should be taken when extrapolations to other species or environmental situations are attempted...