Nicola Decaro

Summary

Affiliation: University of Bari
Country: Italy

Publications

  1. ncbi Specific identification of feline panleukopenia virus and its rapid differentiation from canine parvoviruses using minor groove binder probes
    Nicola Decaro
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy
    J Virol Methods 147:67-71. 2008
  2. ncbi Duplex real-time polymerase chain reaction for simultaneous detection and quantification of Anaplasma marginale and Anaplasma centrale
    Nicola Decaro
    Department of Public Health and Zootechnics, Faculty of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy
    J Vet Diagn Invest 20:606-11. 2008
  3. ncbi Canine parvovirus infection: which diagnostic test for virus?
    Costantina Desario
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine of Bari, s p per Casamassima km 3, 70010 Valenzano Ba, Italy
    J Virol Methods 126:179-85. 2005
  4. ncbi Tissue distribution of the antigenic variants of canine parvovirus type 2 in dogs
    Nicola Decaro
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy
    Vet Microbiol 121:39-44. 2007
  5. doi A pantropic canine coronavirus genetically related to the prototype isolate CB/05
    Nicola Decaro
    Department of Veterinary Public Health, Faculty of Veterinary Medicine, Valenzano, Bari, Italy
    Vet Microbiol 159:239-44. 2012
  6. doi Experimental infection of cattle, sheep and pigs with 'Hobi'-like pestivirus
    Nicola Decaro
    Department of Veterinary Public Health, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy
    Vet Microbiol 155:165-71. 2012
  7. doi Canine parvovirus--a review of epidemiological and diagnostic aspects, with emphasis on type 2c
    Nicola Decaro
    Department of Veterinary Public Health, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy
    Vet Microbiol 155:1-12. 2012
  8. doi A nested PCR approach for unambiguous typing of pestiviruses infecting cattle
    Nicola Decaro
    Department of Veterinary Public Health, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy
    Mol Cell Probes 26:42-6. 2012
  9. pmc Fecal immunoglobulin A antibodies in dogs infected or vaccinated with canine coronavirus
    Nicola Decaro
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine, University of Bari, Bari, Italy
    Clin Diagn Lab Immunol 11:102-5. 2004
  10. ncbi Quantitation of canine coronavirus RNA in the faeces of dogs by TaqMan RT-PCR
    Nicola Decaro
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine of Bari, s p per Casamassima km 3, Valenzano, Bari 70010, Italy
    J Virol Methods 119:145-50. 2004

Detail Information

Publications121 found, 100 shown here

  1. ncbi Specific identification of feline panleukopenia virus and its rapid differentiation from canine parvoviruses using minor groove binder probes
    Nicola Decaro
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy
    J Virol Methods 147:67-71. 2008
    ..Using this assay for extensive molecular surveys will provide precise information on the real circulation of the CPV antigenic variants, including the new variant 2c, in cat population worldwide...
  2. ncbi Duplex real-time polymerase chain reaction for simultaneous detection and quantification of Anaplasma marginale and Anaplasma centrale
    Nicola Decaro
    Department of Public Health and Zootechnics, Faculty of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy
    J Vet Diagn Invest 20:606-11. 2008
    ..even if present simultaneously in the same blood samples. Such an assay could be used in pathogenesis studies on bovine acute anaplasmosis...
  3. ncbi Canine parvovirus infection: which diagnostic test for virus?
    Costantina Desario
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine of Bari, s p per Casamassima km 3, 70010 Valenzano Ba, Italy
    J Virol Methods 126:179-85. 2005
    ..In sum, out of the 68 strains analysed, 26 were characterised as CPV-2a, 18 as CPV-2b and 24 as a CPV-2 Glu-426 mutant recently identified in Italy...
  4. ncbi Tissue distribution of the antigenic variants of canine parvovirus type 2 in dogs
    Nicola Decaro
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy
    Vet Microbiol 121:39-44. 2007
    ..Surprisingly, the nervous tissue was found to contain considerable amounts of CPV nucleic acid. Similar patterns of tissue distribution were observed in all the examined dogs irrespective of the antigenic variant causing the disease...
  5. doi A pantropic canine coronavirus genetically related to the prototype isolate CB/05
    Nicola Decaro
    Department of Veterinary Public Health, Faculty of Veterinary Medicine, Valenzano, Bari, Italy
    Vet Microbiol 159:239-44. 2012
    ..These findings seem to indicate that strain 450/07 is able to spread to internal organs (mainly lymphoid tissues), causing lymphopenia but inducing a mild disease...
  6. doi Experimental infection of cattle, sheep and pigs with 'Hobi'-like pestivirus
    Nicola Decaro
    Department of Veterinary Public Health, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy
    Vet Microbiol 155:165-71. 2012
    ..Nevertheless, the presence of detectable antibodies by virus neutralisation accounted for a successful, albeit limited infection of these animals...
  7. doi Canine parvovirus--a review of epidemiological and diagnostic aspects, with emphasis on type 2c
    Nicola Decaro
    Department of Veterinary Public Health, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy
    Vet Microbiol 155:1-12. 2012
    ..Continuous surveillance will help assess whether there is a real need to update currently available vaccines and diagnostic tests...
  8. doi A nested PCR approach for unambiguous typing of pestiviruses infecting cattle
    Nicola Decaro
    Department of Veterinary Public Health, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy
    Mol Cell Probes 26:42-6. 2012
    ....
  9. pmc Fecal immunoglobulin A antibodies in dogs infected or vaccinated with canine coronavirus
    Nicola Decaro
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine, University of Bari, Bari, Italy
    Clin Diagn Lab Immunol 11:102-5. 2004
    ..A relationship between the level of fecal IgAs to CCV and the degree of protection against CCV infection was observed...
  10. ncbi Quantitation of canine coronavirus RNA in the faeces of dogs by TaqMan RT-PCR
    Nicola Decaro
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine of Bari, s p per Casamassima km 3, Valenzano, Bari 70010, Italy
    J Virol Methods 119:145-50. 2004
    ..The high sensitivity, simplicity and reproducibility of the CCoV fluorogenic RT-PCR assay, combined with its wide dynamic range and high throughput, make this method especially suitable for efficacy trials on CCoV vaccines...
  11. ncbi A real-time PCR assay for rapid detection and quantitation of canine parvovirus type 2 in the feces of dogs
    Nicola Decaro
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy
    Vet Microbiol 105:19-28. 2005
    ..Comparison between these different techniques revealed that real-time PCR is more sensitive than HA and conventional gel-based PCR, allowing to detect low viral titers of CPV-2 in infected dogs...
  12. ncbi Virological and molecular characterization of a mammalian orthoreovirus type 3 strain isolated from a dog in Italy
    Nicola Decaro
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy
    Vet Microbiol 109:19-27. 2005
    ..77%) observed in dogs...
  13. ncbi Genotype-specific fluorogenic RT-PCR assays for the detection and quantitation of canine coronavirus type I and type II RNA in faecal samples of dogs
    Nicola Decaro
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine of Bari, Strada prov per Casamassima Km 3, 70010 Valenzano, Bari, Italy
    J Virol Methods 130:72-8. 2005
    ..At the same time, dogs infected experimentally shed type I RNA with higher titres with respect to type II...
  14. ncbi Identification of a feline coronavirus type I strain from a cat with feline infectious peritonitis by RT-pCR and phylogenetic analysis
    Marco Campolo
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine, Valenzano Ba, Italy
    New Microbiol 28:127-33. 2005
    ..The RT-PCR results were confirmed by sequence analysis of the amplified products. Phylogeny carried out on fragments of the M and S genes showed that the FCoV strain segregates with typical type I FCoVs...
  15. ncbi Maternally-derived antibodies in pups and protection from canine parvovirus infection
    Nicola Decaro
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy
    Biologicals 33:261-7. 2005
    ..These findings are in contrast with the MDA titre (HI > or = 80) usually considered fully protective for CPV infection, and suggest the need for revision of current vaccination programmes for pups...
  16. ncbi Characterisation of the canine parvovirus type 2 variants using minor groove binder probe technology
    Nicola Decaro
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine of Bari, 70010 Valenzano, Bari, Italy
    J Virol Methods 133:92-9. 2006
    ..These results make the established MGB probe assays an attractive tool for direct and rapid characterisation of type 2a, 2b and 2c CPVs...
  17. ncbi New approaches for the molecular characterization of canine parvovirus type 2 strains
    N Decaro
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano Bari, Italy
    J Vet Med B Infect Dis Vet Public Health 52:316-9. 2005
    ..Therefore, the MGB probe assays represent a quick, reliable tool for prediction of CPV-2 antigen specificity, with regard to the more time-consuming assays currently used...
  18. ncbi A minor groove binder probe real-time PCR assay for discrimination between type 2-based vaccines and field strains of canine parvovirus
    Nicola Decaro
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano Bari, Italy
    J Virol Methods 136:65-70. 2006
    ....
  19. ncbi Diagnostic tools based on minor groove binder probe technology for rapid identification of vaccinal and field strains of canine parvovirus type 2b
    Nicola Decaro
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy
    J Virol Methods 138:10-6. 2006
    ..These new assays will help resolution of the diagnostic problems related to the detection of a type 2b strain in the faeces of dogs shortly after the administration of a type 2b vaccine...
  20. ncbi Occurrence of severe gastroenteritis in pups after canine parvovirus vaccine administration: a clinical and laboratory diagnostic dilemma
    Nicola Decaro
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy
    Vaccine 25:1161-6. 2007
    ....
  21. ncbi First detection of canine parvovirus type 2c in pups with haemorrhagic enteritis in Spain
    N Decaro
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano Bari, Italy
    J Vet Med B Infect Dis Vet Public Health 53:468-72. 2006
    ..We suggest that this new antigenic variant of CPV-2 could spread throughout Europe and that there is a subsequent need to update current CPV vaccines...
  22. ncbi Infectious canine hepatitis: an "old" disease reemerging in Italy
    N Decaro
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy
    Res Vet Sci 83:269-73. 2007
    ..The present study shows that CAV-1 is currently circulating in the Italian dog population and that vaccination is still required...
  23. ncbi Serological and molecular evidence that canine respiratory coronavirus is circulating in Italy
    Nicola Decaro
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy
    Vet Microbiol 121:225-30. 2007
    ..The results of the present study show that CRCoV is present also in continental Europe, although further studies are required to determine the real pathogenic potential of the virus...
  24. ncbi Molecular characterisation of the virulent canine coronavirus CB/05 strain
    Nicola Decaro
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, Valenzano Bari, Italy
    Virus Res 125:54-60. 2007
    ....
  25. ncbi Severe outbreak of bovine coronavirus infection in dairy cattle during the warmer season
    Nicola Decaro
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy
    Vet Microbiol 126:30-9. 2008
    ....
  26. pmc The study molecular epidemiology of canine parvovirus, Europe
    Nicola Decaro
    Department of Animal Health and Well Being, Faculty of Veterinary, University of Bari, Bari, Italy
    Emerg Infect Dis 13:1222-4. 2007
    ..Molecular method assessment of the distribution of the CPV variants in Europe showed that the new variant CPV-2c is widespread in Europe and that the viruses are distributed in different countries...
  27. ncbi Experimental infection of dogs with a novel strain of canine coronavirus causing systemic disease and lymphopenia
    Nicola Decaro
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy
    Vet Microbiol 128:253-60. 2008
    ..The present study confirms that strain CB/05 is highly pathogenic for dogs, being able to induce a severe disease (and in some cases the death) even in experimental conditions...
  28. ncbi Respiratory disease associated with bovine coronavirus infection in cattle herds in Southern Italy
    Nicola Decaro
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine of Bari, s p per Casamassima km 3, 70010 Valenzano, Bari, Italy
    J Vet Diagn Invest 20:28-32. 2008
    ..10 x 10(2) and 7.50 x 10(7) RNA copies/microl of template. Bovine coronavirus was isolated from respiratory specimens from all outbreaks except outbreak 1, in which real-time RT-PCR found very low viral titers in nasal swabs...
  29. ncbi Evidence for immunisation failure in vaccinated adult dogs infected with canine parvovirus type 2c
    Nicola Decaro
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine of Bari, Valenzano, Bari, Italy
    New Microbiol 31:125-30. 2008
    ....
  30. doi Detection of bovine coronavirus using a TaqMan-based real-time RT-PCR assay
    Nicola Decaro
    Department of Public Health and Animal Sciences, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy
    J Virol Methods 151:167-71. 2008
    ..The rapidity and high throughput of the BCoV TaqMan assay makes this method a powerful tool for a sensitive and specific diagnosis of BCoV infection in cattle...
  31. doi An update on canine coronaviruses: viral evolution and pathobiology
    Nicola Decaro
    Department of Public Health and Animal Sciences, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy
    Vet Microbiol 132:221-34. 2008
    ..The significances of the newly acquired information for the canine health status and prophylaxis programmes are also discussed...
  32. doi Genetic analysis of feline panleukopenia viruses from cats with gastroenteritis
    N Decaro
    Department of Public Health and Animal Sciences, Faculty of Veterinary Medicine, Strada per Casamassima Km 3, 70010 Valenzano Ba, Italy
    J Gen Virol 89:2290-8. 2008
    ....
  33. pmc Recombinant canine coronaviruses related to transmissible gastroenteritis virus of Swine are circulating in dogs
    Nicola Decaro
    Department of Public Health and Animal Sciences, Faculty of Veterinary Medicine, Valenzano Bari, Italy
    J Virol 83:1532-7. 2009
    ..By virus neutralization tests, antigenic differences between reference and TGEV-like CCoVs were found. Our data support the potential recombinant origin of the TGEV-like CCoVs...
  34. doi Genetic analysis of canine parvovirus type 2c
    Nicola Decaro
    Department of Public Health and Animal Sciences, Valenzano, Bari, Italy
    Virology 385:5-10. 2009
    ..Analysis of the selection pressure driving CPV evolution confirmed that the VP2 gene is under purifying selection. The emergence and global spread of the new CPV variant provides an interesting model to better understand virus evolution...
  35. ncbi A candidate modified-live bovine coronavirus vaccine: safety and immunogenicity evaluation
    Nicola Decaro
    Department of Public Health and Animal Sciences, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy
    New Microbiol 32:109-13. 2009
    ..Sequence analysis of the S gene of wild-type and cell-adapted 438/06-TN strain detected 10 nucleotide changes, 9 of which were non-synonymous...
  36. doi Detection of canine parvovirus type 2c by a commercially available in-house rapid test
    Nicola Decaro
    Department of Veterinary Public Health, Faculty of Veterinary Medicine, Strada per Casamassima Km 3, 70010 Valenzano Bari, Italy
    Vet J 184:373-5. 2010
    ..However, considering the sensitivity limits of the in-house tests that have been observed previously, negative results from the in-house test kit should be confirmed by PCR-based methods...
  37. doi Immunity after natural exposure to enteric canine coronavirus does not provide complete protection against infection with the new pantropic CB/05 strain
    Nicola Decaro
    Department of Veterinary Public Health, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano Bari, Italy
    Vaccine 28:724-9. 2010
    ....
  38. doi Western European epidemiological survey for parvovirus and coronavirus infections in dogs
    Nicola Decaro
    Department of Veterinary Public Health, Faculty of Veterinary Medicine, Strada per Casamassima Km 3, 70010 Valenzano Bari, Italy
    Vet J 187:195-9. 2011
    ..Except for the UK, CCoV genotype I was identified in all European countries involved in the survey, albeit at a lower prevalence rates than CCoV genotype II...
  39. pmc Recombinant canine coronaviruses in dogs, Europe
    Nicola Decaro
    Department of Veterinary Public Health, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy
    Emerg Infect Dis 16:41-7. 2010
    ..87% and 3.45%, respectively). Sequence and phylogenetic analyses showed that the CCoV-IIb strains were related to prototype TGEV-like strains in the 5' and the 3' ends of the spike protein gene...
  40. doi Characterisation of canine parvovirus strains isolated from cats with feline panleukopenia
    Nicola Decaro
    Department of Veterinary Public Health, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy
    Res Vet Sci 89:275-8. 2010
    ..Whether and to which extent the FPL vaccines can protect cats adequately from the antigenic variants of CPV-2 should be assessed...
  41. doi Characterisation of bubaline coronavirus strains associated with gastroenteritis in water buffalo (Bubalus bubalis) calves
    Nicola Decaro
    Department of Veterinary Public Health, Faculty of Veterinary Medicine, Strada per Casamassima Km 3, Valenzano, Bari, Italy
    Vet Microbiol 145:245-51. 2010
    ..The present report shows that albeit genetically heterogeneous, the different BuCoV strains possess a common biological pattern which is different from most BCoV and BCoV-like isolates...
  42. doi Development and validation of a real-time PCR assay for specific and sensitive detection of canid herpesvirus 1
    Nicola Decaro
    Department of Veterinary Public Health, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano Bari, Italy
    J Virol Methods 169:176-80. 2010
    ....
  43. doi Canine parvovirus type 2c infection in a kitten associated with intracranial abscess and convulsions
    Nicola Decaro
    Department of Veterinary Public Health, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy
    J Feline Med Surg 13:231-6. 2011
    ..Continuous surveillance is needed to monitor future spreading of this CPV-2c mutant, and any associated clinical signs, in the dog and cat population...
  44. doi Immunogenicity and protective efficacy in dogs of an MF59™-adjuvanted vaccine against recombinant canine/porcine coronavirus
    Nicola Decaro
    Department of Veterinary Public Health, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy
    Vaccine 29:2018-23. 2011
    ....
  45. pmc Atypical pestivirus and severe respiratory disease in calves, Europe
    Nicola Decaro
    Department of Veterinary Public Health, Faculty of Veterinary Medicine of Bari, Valenzano, Bari, Italy
    Emerg Infect Dis 17:1549-52. 2011
    ..Isolation of this novel virus from a clinical outbreak may have implications for cattle health and prophylactic programs...
  46. doi Canine coronavirus: not only an enteric pathogen
    Nicola Decaro
    Department of Veterinary Public Health, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy
    Vet Clin North Am Small Anim Pract 41:1121-32. 2011
    ..It also describes pantropic CCoV-induced disease reproduced under experimental conditions...
  47. pmc Hobi-like pestivirus in aborted bovine fetuses
    Nicola Decaro
    Department of Veterinary Public Health, Faculty of Veterinary Medicine, Valenzano Bari, Italy
    J Clin Microbiol 50:509-12. 2012
    ..These findings may have important implications for the pestivirus control/eradication programs in cattle herds...
  48. ncbi Genetic diversity of a canine coronavirus detected in pups with diarrhoea in Italy
    Annamaria Pratelli
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine of Bari, s p per Casamassima km 3, 70010, Valenzano, Bari, Italy
    J Virol Methods 110:9-17. 2003
    ..The evident genetic divergence between the reference CCoV strains and the newly identified Elmo/02-like CCoVs strongly suggests that a novel genotype of CCoV is widespread in the dog population...
  49. ncbi Biological and genetic analysis of a bovine-like coronavirus isolated from water buffalo (Bubalus bubalis) calves
    Nicola Decaro
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine, Valenzano Bari, Italy
    Virology 370:213-22. 2008
    ..In conclusion, our data support the existence of a host-range variant of BCoV associated with enteritis in buffaloes...
  50. ncbi An outbreak of equine influenza virus in vaccinated horses in Italy is due to an H3N8 strain closely related to recent North American representatives of the Florida sub-lineage
    Vito Martella
    Department of Animal Health and Well Being, University of Bari, Valenzano, Bari, Italy
    Vet Microbiol 121:56-63. 2007
    ..Four changes were localized in the antigenic regions C-D and likely accounted for the vaccine failure...
  51. pmc Two genotypes of canine coronavirus simultaneously detected in the fecal samples of dogs with diarrhea
    Annamaria Pratelli
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine of Bari, Bari, Italy
    J Clin Microbiol 42:1797-9. 2004
    ..The isolates in 10 samples were recognized as CCoV type I, and the isolates in 6 samples were recognized as CCoV type II, while isolates of both genotypes were simultaneously detected in 53 samples...
  52. pmc Evaluation of the antigenic relationships among canine parvovirus type 2 variants
    Alessandra Cavalli
    Department of Animal Health and Well Being, University of Bari, Valenzano, Bari, Italy
    Clin Vaccine Immunol 15:534-9. 2008
    ..The observed antigenic differences might drive selection of CPV strains by generating differential immune pressure in the canine population, which raises concerns about vaccine efficacy...
  53. pmc Genetic heterogeneity and recombination in canine noroviruses
    Vito Martella
    Department of Veterinary Public Health, University of Bari, Valenzano, Bari, Italy
    J Virol 83:11391-6. 2009
    ..9% nucleotide identity and 98.9% amino acid identity) identified from oysters in Japan, indicating that similar viruses may be common environmental contaminants...
  54. pmc Persistent infection caused by Hobi-like pestivirus
    Nicola Decaro
    Department of Veterinary Medicine, University of Bari, Valenzano, Italy
    J Clin Microbiol 51:1241-3. 2013
    ..This report provides new insights into the pathogenesis of the emerging pestivirus...
  55. ncbi Clinical and virological findings in pups naturally infected by canine parvovirus type 2 Glu-426 mutant
    Nicola Decaro
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine of Bari, Valenzano, Bari, Italy
    J Vet Diagn Invest 17:133-8. 2005
    ..By real-time PCR, the highest viral DNA titers were detected in the feces of both litters at day 10, reaching median values of more than 10(10) DNA copies/mg of feces...
  56. ncbi Prevalence of group C rotaviruses in weaning and post-weaning pigs with enteritis
    Vito Martella
    Department of Animal Health and Wellbeing, Faculty of Veterinary Medicine of Bari, s p per Casamassima km 3, 70010 Valenzano, Bari, Italy
    Vet Microbiol 123:26-33. 2007
    ....
  57. ncbi Identification of coronaviruses in dogs that segregate separately from the canine coronavirus genotype
    Annamaria Pratelli
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine of Bari, s p per Casamassima km 3, 70010, Valenzano, Bari, Italy
    J Virol Methods 107:213-22. 2003
    ..The reliability of the classification results was confirmed by repeating the phylogenetic analysis with nucleotide and amino acid sequences from multiple genomic regions...
  58. ncbi Genetic heterogeneity in the VP7 of group C rotaviruses
    Vito Martella
    Department of Animal Health and Wellbeing, Faculty of Veterinary Medicine of Bari, Valenzano, Bari, Italy
    Virology 367:358-66. 2007
    ..These findings provide the need for future epidemiological surveys and warrant studies to investigate the pathogenic potential of these novel GCRVs in pigs...
  59. ncbi Molecular characterization of the VP4, VP6, VP7, and NSP4 genes of lapine rotaviruses identified in italy: emergence of a novel VP4 genotype
    Vito Martella
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine of Bari, Italy
    Virology 314:358-70. 2003
    ..This report confirms the worldwide genetic constellations of LRVs and identifies a novel VP4 genotype in rabbits, tentatively proposed as genotype P[22]...
  60. pmc Norovirus in captive lion cub (Panthera leo)
    Vito Martella
    University of Bari, Valenzano, Bari, Italy
    Emerg Infect Dis 13:1071-3. 2007
    ..We report the identification of a novel enteric calicivirus, genetically related to human noroviruses of genogroup IV, in a lion cub that died of severe hemorrhagic enteritis...
  61. doi Molecular characterization of a canine respiratory coronavirus strain detected in Italy
    Alessio Lorusso
    Department of Public Health, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy
    Virus Res 141:96-100. 2009
    ..9 kDa non-structural protein and a truncated form of the 4.8 kDa protein, whereas CRCoV 4182 has a unique 8.8 kDa protein...
  62. doi Long-term immunogenicity and protection against Mycoplasma agalactiae induced by an oil adjuvant vaccine in sheep
    Domenico Buonavoglia
    Department of Veterinary Public Health, Faculty of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy
    Res Vet Sci 88:16-9. 2010
    ..agalactiae in all groups of animals irrespective of the time of challenge after booster administration...
  63. doi Hobi-like pestivirus: both biotypes isolated from a diseased animal
    Nicola Decaro
    Department of Veterinary Public Health, University of Bari, Valenzano Bari, Italy
    J Gen Virol 93:1976-83. 2012
    ..This is the first study, to our knowledge, reporting the isolation and molecular characterization of a Hobi-like virus pair...
  64. ncbi Detection of infectious canine parvovirus type 2 by mRNA real-time RT-PCR
    Gabriella Elia
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine of Bari, s p per Casamassima km 3, 70010 Valenzano, Bari, Italy
    J Virol Methods 146:202-8. 2007
    ..The assay was then applied to determine the mRNA loads in the tissues of dogs naturally infected by CPV-2. mRNA was detected in a variety of tissues, including the central nervous system...
  65. ncbi Development of a real-time PCR for the detection and quantitation of caprine herpesvirus 1 in goats
    Gabriella Elia
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine, University of Bari, Sp Casamassima km 3, 70010 Valenzano, Italy
    J Virol Methods 148:155-60. 2008
    ....
  66. doi An ELISA based on recombinant spike protein S for the detection of antibodies to transmissible gastroenteritis virus of swine-like canine coronaviruses
    Gabriella Elia
    Department of Veterinary Public Health, University of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy
    J Virol Methods 163:309-12. 2010
    ..As classical CCoV-II and TEGV-like CCoVs are related antigenically, the recombinant S' ELISA is a useful method to investigate serologically the prevalence of TGEV-like CCoVs in dogs...
  67. ncbi Cloning and expression of two fragments of the S gene of canine coronavirus type I
    Annamaria Pratelli
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine, University of Bari, Strada per Casamassima, km 3, 70010 Valenzano, Bari, Italy
    J Virol Methods 117:61-5. 2004
    ..As CCoV type I seems to be not cultivable in cell cultures, the recombinant fragments of the S protein represent a unique method to study, preliminarily, the immunological and the pathogenetic characteristics of this new virus...
  68. pmc Immunogenicity of an intranasally administered modified live canine parvovirus type 2b vaccine in pups with maternally derived antibodies
    Vito Martella
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine, University of Bari, s p per Casamassima km 3, 70010 Valenzano, Bari, Italy
    Clin Diagn Lab Immunol 12:1243-5. 2005
    ..The vaccine induced seroconversion in 100% of pups with MDA titers of < or = 80 and in 51.6% of pups with titers between 160 and 320...
  69. pmc Canine coronavirus highly pathogenic for dogs
    Canio Buonavoglia
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine, University of Bari, Bari, Italy
    Emerg Infect Dis 12:492-4. 2006
    ..We report an outbreak of fatal disease in puppies caused by a pathogenic variant of CCoV that was isolated from organs with severe lesions...
  70. ncbi Evaluation of the innate immune response in pups during canine parvovirus type 1 infection
    N Decaro
    Department of Health and Animal Well being, Faculty of Veterinary Medicine, 70010 Valenzano, Bari, Italy
    New Microbiol 25:291-8. 2002
    ..In pup B, killing exerted by PMN was absent at T0, then increased but again dropped below normal ranges at T3. The described alterations of phagocytic functions may be regarded as possible viral mechanisms of immune evasion...
  71. ncbi Canine distemper and related diseases: report of a severe outbreak in a kennel
    N Decaro
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine, Valenzano, BA, Italy
    New Microbiol 27:177-81. 2004
    ..Furthermore, canine coronavirus was detected by PCR in the intestinal content of this pup, suggesting a multifactorial aetiology of the outbreak...
  72. ncbi Relationships among porcine and human P[6] rotaviruses: evidence that the different human P[6] lineages have originated from multiple interspecies transmission events
    Vito Martella
    Dipartimento di Sanità e Benessere Animale, Facoltà di Medicina Veterinaria di Bari, s p per Casamassima km 3, 70010 Valenzano, Bari, Italy
    Virology 344:509-19. 2006
    ....
  73. ncbi Intravaginal administration of an inactivated vaccine prevents lesions induced by caprine herpesvirus-1 in goats
    Michele Camero
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine, University of Bari, Strada P le per Casamassima Km 3, 70010 Valenzano, Italy
    Vaccine 25:1658-61. 2007
    ..Even without adjuvants, prolonged mucosal vaccination induced production of secretory IgA and provided significant clinical protection...
  74. ncbi Characterization of a canine parvovirus strain isolated from an adult dog
    A Cavalli
    Department of Health and Animal Welfare, Faculty of Veterinary Medicine, Bari, Italy
    New Microbiol 24:239-42. 2001
    ..Characterization of the virus, by an indirect immunofluorescence assay with monoclonal antibodies, confirmed the antigenic CPV-2b-like pattern of the nonhemagglutinating virus...
  75. ncbi Evaluation of antibody response to canine coronavirus infection in dogs by Western Blotting analysis
    G Elia
    Department of Health and Animal Well being, Faculty of Veterinary Medicine, Bari, Italy
    New Microbiol 25:275-80. 2002
    ..The limited response to S protein may explain the poor protection of dogs and the possibility of persisting infection...
  76. ncbi M gene evolution of canine coronavirus in naturally infected dogs
    A Pratelli
    Department of Animal Health and Wellbeing, Faculty of Veterinary Medicine, University of Bari, s p per Casamassima km 3, 70010 Valenzano, Italy
    Vet Rec 151:758-61. 2002
    ....
  77. ncbi Analysis of the capsid protein gene of a feline-like calicivirus isolated from a dog
    Vito Martella
    Faculty of Veterinary Medicine, Department of Animal Health and Well Being, Bari, Italy
    Vet Microbiol 85:315-22. 2002
    ....
  78. ncbi Analysis of antibody response in goats to caprine herpesvirus 1
    Maria Tempesta
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine, University of Bari, Strada Provinciale per Casamassima Km 3, 70010 Valenzano, Italy
    Biologicals 33:283-7. 2005
    ..The neutralizing antibody titers and the pattern of antibody reactivity were hypothesized to modulate the reactivation and re-excretion process of CpHV.1...
  79. ncbi Detection of equine herpesvirus type 1 by real time PCR
    Gabriella Elia
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine of Bari, 70010 Valenzano, Bari, Italy
    J Virol Methods 133:70-5. 2006
    ..The high sensitivity and reproducibility of the EHV-1-specific fluorogenic PCR assay, combined with the wide dynamic range and the high throughput, make this method suitable for diagnostic and research applications...
  80. ncbi Detection of canine distemper virus in dogs by real-time RT-PCR
    Gabriella Elia
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine of Bari, s p per Casamassima km 3, 70010 Valenzano, Bari, Italy
    J Virol Methods 136:171-6. 2006
    ..Urines, tonsil, conjunctival swabs and whole blood were found to contain high virus loads and therefore proved to be suitable targets for detection of canine distemper virus RNA...
  81. ncbi Genotyping canine distemper virus (CDV) by a hemi-nested multiplex PCR provides a rapid approach for investigation of CDV outbreaks
    Vito Martella
    Department of Animal Health and Well Being, University of Bari, Valenzano, Bari, Italy
    Vet Microbiol 122:32-42. 2007
    ..The method could be useful for molecular epidemiological studies of CDV, providing a tool for large-scale studies, and for the diagnosis of vaccine-related disease...
  82. ncbi Potent inhibition of genital herpesvirus infection in goats by cidofovir
    Maria Tempesta
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine, University of Bari, Valenzano, Bari, Italy
    Antivir Ther 12:977-9. 2007
    ..Like human herpesvirus 2 (HHV-2) in humans, infection by caprine herpesvirus 1 in goats is associated with genital lesions, and this provides a unique model to study the efficacy and effects of anti-herpetic drugs...
  83. ncbi An oil-emulsion vaccine induces full-protection against Mycoplasma agalactiae infection in sheep
    Domenico Buonavoglia
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine, University of Bari, Italy
    New Microbiol 31:117-23. 2008
    ..agalactiae...
  84. doi Identification of tuna species in commercial cans by minor groove binder probe real-time polymerase chain reaction analysis of mitochondrial DNA sequences
    Valentina Terio
    Department of Veterinary Public Health, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy
    Mol Cell Probes 24:352-6. 2010
    ..These results make the established MGB probe assays an attractive tool for direct and rapid species identification in canned tuna...
  85. pmc Environmental monitoring and analysis of faecal contamination in an urban setting in the city of Bari (Apulia region, Italy): health and hygiene implications
    Elvira Tarsitano
    Department of Veterinary Public Health, Faculty of Veterinary Medicine, University of Bari, Str Prov Casamassima km 3, 70010, Valenzano, Bari, Italy
    Int J Environ Res Public Health 7:3972-86. 2010
    ..Our study showed that public areas are often contaminated by potentially zoonotic pathogens...
  86. ncbi Use of real-time RT-PCR as a rapid molecular approach for differentiation of field and vaccine strains of bluetongue virus serotypes 2 and 9
    Gabriella Elia
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine of Bari, Valenzano Bari, Italy
    Mol Cell Probes 22:38-46. 2008
    ..Both the assays were found to be highly sensitive and reproducible, ensuring correct serotype characterisation and prediction of the origin of the strains, as confirmed by characterisation using virus neutralisation and sequencing...
  87. ncbi Detection of Brucella canis in a dog in Italy
    Marialaura Corrente
    Department of Public Health, Faculty of Veterinary Medicine, University of Bari, Italy
    New Microbiol 33:337-41. 2010
    ..B. canis was detected by PCR in biological fluids and tissues of the animal, while antibodies to B. canis were found in the serum, providing additional strong evidence for the circulation of B. canis in Italy...
  88. ncbi Malignant catarrhal fever in a captive American bison (Bison bison) in Italy
    Marco Campolo
    Department of Public Health and Animal Sciences, Faculty of Veterinary Medicine of Bari, Strada per Casamassima Km 3, Valenzano, Bari, Italy
    J Vet Diagn Invest 20:843-6. 2008
    ..To the authors' knowledge, this is the first MCF case in captive ruminants in Italy and the second confirmed case in captive bison of European zoos...
  89. pmc Canine parvovirus (CPV) vaccination: comparison of neutralizing antibody responses in pups after inoculation with CPV2 or CPV2b modified live virus vaccine
    A Pratelli
    Department of Health and Animal Well being, Faculty of Veterinary Medicine, University of Bari, Bari, Italy
    Clin Diagn Lab Immunol 8:612-5. 2001
    ..These data may suggest an advantage for new vaccines, considering that most presently licensed vaccines are produced with CPV2, which no longer exists in the dog population...
  90. ncbi Variation of the sequence in the gene encoding for transmembrane protein M of canine coronavirus (CCV)
    A Pratelli
    Department of Health and Animal Well being, Faculty of Veterinary Medicine, 70010 Valenzano, Bari, Italy
    Mol Cell Probes 15:229-33. 2001
    ..Moreover, the nucleotide substitutions occurring over the whole fragment of the five samples analysed were similar...
  91. ncbi Isolation and genetic characterization of two G3P5A[3] canine rotavirus strains in Italy
    V Martella
    Faculty of Veterinary Medicine, Department of Animal Health and Wellbeing, University of Bari, Bari, Italy
    J Virol Methods 96:43-9. 2001
    ..The isolation in Italy of canine rotaviruses displaying the same VP7 and VP4 specificities as in the USA and in Japan, suggests that the G3 and P5A[3] types are highly conserved among canine rotavirus strains...
  92. ncbi Evidence for evolution of canine parvovirus type 2 in Italy
    C Buonavoglia
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine of Bari, s p per Casamassima km 3, 70010 Valenzano, Bari, Italy
    J Gen Virol 82:3021-5. 2001
    ..The failure of established typing methods to distinguish this antigenic variant was overcome by the development of an RFLP assay...
  93. ncbi Detection and genetic characterization of canine distemper virus (CDV) from free-ranging red foxes in Italy
    V Martella
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine, Valenzano Ba, Italy
    Mol Cell Probes 16:77-83. 2002
    ....
  94. ncbi Efficacy of an inactivated canine coronavirus vaccine in pups
    A Pratelli
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine, Valenzano, Bari, Italy
    New Microbiol 26:151-5. 2003
    ..The two non vaccinated pups displayed mild diarrhoea at day post-challenge 4 and shed the challenge-virus for 14 and 15 days respectively, by virus isolation, and for 22 and 24 days respectively, by PCR assay...
  95. ncbi First two confirmed cases of malignant catarrhal fever in Italy
    N Decaro
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine, 70010 Valenzano, BA, Italy
    New Microbiol 26:339-44. 2003
    ..OvHV-2 viral DNA was detected by a PCR test performed on peripheral blood leucocytes (PBL) and tissue samples from both the animals, completing the definitive diagnosis of MCF...
  96. pmc Molecular analysis of the VP7, VP4, VP6, NSP4, and NSP5/6 genes of a buffalo rotavirus strain: identification of the rare P[3] rhesus rotavirus-like VP4 gene allele
    V Martella
    Department of Animal Health and Wellbeing, Faculty of Veterinary Medicine of Bari, Bari, Italy
    J Clin Microbiol 41:5665-75. 2003
    ..The detection of the rare P[3] genotype in ruminants provides additional evidence for the wide genetic and antigenic diversity of group A rotaviruses...
  97. ncbi A severe dual infection by feline panleukopenia virus and feline calicivirus in an adult cat
    M Camero
    Department of Health and Animal Well being, Faculty of Veterinary Medicine of Bari, Valenzano, Bari, Italy
    New Microbiol 27:79-82. 2004
    ..Antibodies to both the viruses were detected in the serum. The severity of the disease induced by the mixed viral infection highlights the need for intensifying FPV vaccination in cats...
  98. ncbi Safety and efficacy of a modified-live canine coronavirus vaccine in dogs
    A Pratelli
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine, Strada per Casamassima Km 3, 70010 Valenzano, Bari, Italy
    Vet Microbiol 99:43-9. 2004
    ..The two control dogs displayed moderate clinical signs and the virus was detected by VI for 14.5 median days starting from day 3 post-challenge (dpc 3) and by PCR assay for 23 median days starting from dpc 1...
  99. ncbi Sequence analysis of the VP7 and VP4 genes identifies a novel VP7 gene allele of porcine rotaviruses, sharing a common evolutionary origin with human G2 rotaviruses
    V Martella
    Dipartimento di Sanità e Benessere Animale, Facoltà di Medicina Veterinaria di Bari, Valenzano, Italy
    Virology 337:111-23. 2005
    ....
  100. ncbi Lapine rotaviruses of the genotype P[22] are widespread in Italian rabbitries
    V Martella
    Department of Animal Health and Well Being, University of Bari, Valenzano, Bari, Italy
    Vet Microbiol 111:117-24. 2005
    ..However, one of the P[14],G3 strains possessed a super-short pattern. Altogether, these data highlight the epidemiological relevance of the P[22] LRVs in Italian rabbitries...
  101. ncbi Surveillance activity for canine parvovirus in Italy
    V Martella
    Department of Animal Health and Well Being, Faculty of Veterinary Medicine, University of Bari, s p per Casamassima km 3, 70010 Valenzano, Bari, Italy
    J Vet Med B Infect Dis Vet Public Health 52:312-5. 2005
    ..The identification of CPV-2 variants raises several questions concerning their impact on the efficacy of the current CPV-2 vaccines, based on the original CPV-2 strain that no longer exists in the field...