S Fucile

Summary

Affiliation: University of Rome La Sapienza
Country: Italy

Publications

  1. ncbi request reprint Nicotine modulates the spontaneous synaptic activity in cultured embryonic rat spinal cord interneurons
    Sergio Fucile
    Istituto Pasteur, Fondazione Cenci Bolognetti and Dipartimento di Fisiologia Umana e Farmacologia, Universita La Sapienza, Rome, Italy
    J Neurosci Res 67:329-36. 2002
  2. pmc Effects of cyclothiazide on GluR1/AMPA receptors
    Sergio Fucile
    Istituto Pasteur Fondazione Cenci Bolognetti, Dipartimento di Fisiologia Umana e Farmacologia, Centro di Eccellenza BEMM, Universita di Roma La Sapienza, Piazzale Aldo Moro 5, I00185 Rome, Italy
    Proc Natl Acad Sci U S A 103:2943-7. 2006
  3. ncbi request reprint Ca2+ permeability through rat cloned alpha9-containing nicotinic acetylcholine receptors
    Sergio Fucile
    Istituto Pasteur Fondazione Cenci Bolognetti and Dipartimento di Fisiologia Umana e Farmacologia, Centro di Eccellenza Biologia and Medicina Molecolare, Università di Rome La Sapienza, Italy
    Cell Calcium 39:349-55. 2006
  4. pmc Ca2+ permeability of nicotinic acetylcholine receptors from rat dorsal root ganglion neurones
    Sergio Fucile
    Istituto Pasteur Fondazione Cenci Bolognetti and Dipartimento di Fisiologia Umana e Farmacologia, Centro di Eccellenza Biologia e Medicina Molecolare, Universita di Roma La Sapienza, Roma, Italy
    J Physiol 565:219-28. 2005
  5. ncbi request reprint Nicotinic cholinergic stimulation promotes survival and reduces motility of cultured rat cerebellar granule cells
    S Fucile
    Istituto Pasteur Fondazione Cenci Bolognetti and Dipartimento di Fisiologia Umana e Farmacologia, Centro di Eccellenza Biologia e Medicina Molecolare, Universita di Roma La Sapienza, P le Aldo Moro 5, I 00185 Roma, Italy
    Neuroscience 127:53-61. 2004
  6. ncbi request reprint Ca2+ permeability of nicotinic acetylcholine receptors
    Sergio Fucile
    Dipartimento di Fisiologia Umana e Farmacologia, Universita di Roma La Sapienza, P le Aldo Moro 5, I 00185 Rome, Italy
    Cell Calcium 35:1-8. 2004
  7. ncbi request reprint Fractional Ca(2+) current through human neuronal alpha7 nicotinic acetylcholine receptors
    Sergio Fucile
    Istituto di Fisiologia Umana, Universita di Roma La Sapienza, P le Aldo Moro 5, I 00185 Rome, Italy
    Cell Calcium 34:205-9. 2003
  8. ncbi request reprint Chemokine receptor CXCR2 regulates the functional properties of AMPA-type glutamate receptor GluR1 in HEK cells
    P Lax
    Dipartimento di Fisiologia Umana e Farmacologia, Universita di Roma, Piazzale Aldo Moro 5, I00185, Rome, Italy
    J Neuroimmunol 129:66-73. 2002
  9. ncbi request reprint Ca(2+) permeability of human heteromeric nAChRs expressed by transfection in human cells
    P Lax
    Dipartimento di Fisiologia Umana e Farmacologia, Istituto Pasteur Fondazione Cenci Bolognetti, Universita di Roma La Sapienza, P le Aldo Moro 5, I 00185 Roma, Italy
    Cell Calcium 32:53-8. 2002
  10. ncbi request reprint Serotonin antagonizes the human neuronal alpha7 nicotinic acetylcholine receptor and becomes an agonist after L248T alpha7 mutation
    S Fucile
    Istituto Pasteur Fondazione Cenci Bolognetti, Dipartimento di Fisiologia Umana e Farmacologia, Universita di Roma La Sapienza, Viale Regina Elena 324, I 00161 Rome, Italy
    Neuroscience 110:169-79. 2002

Collaborators

  • Eleonora Palma
  • Flavia Trettel
  • Pedro Lax
  • C Limatola
  • E Aronica
  • R Miledi
  • D Ragozzino
  • Fabrizio Eusebi
  • Maurizio Inghilleri
  • Ammar Al-Chalabi
  • B B Fredholm
  • Francesca Grassi
  • Cristina Roseti
  • Claudia Moriconi
  • Alessia Orlandi
  • Francesca Pagani
  • Maurizio C Capogrossi
  • Elisa Iacovelli
  • Mario Sabatelli
  • Gianpaolo Cantore
  • Giancarlo Di Gennaro
  • Pier Paolo Quarato
  • Vincenzo Esposito
  • Mario Manfredi
  • Katiuscia Martinello
  • Addolorata Mascia
  • Gemma Molinaro
  • Daniele Avitabile
  • Maurizio Pesce
  • Pasquale Fasanaro
  • Laura Lagostena
  • Salvatore Minisola
  • Alfredo Scillitani
  • Maria Lucia Mascia
  • Francesca Gilio
  • Elisabetta Romagnoli
  • Floriana Pichiorri
  • Maria Amalia Di Castro
  • Pietro Tonali
  • Marco Luigetti
  • Rosaria Volpini
  • Roberta Morace
  • Daniela Orteschi
  • Francesca Madia
  • Giovanni Neri
  • Serena Lattante
  • Alessandra Del Grande
  • Amalia Di Castro
  • Catia Lambertucci
  • Fabrizia Sobrero
  • Gloria Cristalli
  • Gabriella Marucci
  • Amelia Conte
  • Marina Murdolo
  • Antonietta Arcella
  • Irene Mancuso
  • Marcella Zollino
  • Silvia Di Angelantonio
  • Giuseppe Marangi
  • Felice Giangaspero
  • Barbara Riozzi
  • Elisa Vigna
  • Michele Simonato
  • Antonella Arcella
  • Marianna Storto
  • Giuseppina Bonanno
  • Giovanni Scambia
  • Giuseppe Battaglia
  • Vanessa Piccari
  • Ferdinando Nicoletti
  • Valeria Bruno
  • Germana Zaccagnini
  • Lucia Cicchillitti
  • Myriam Catalano
  • Fabio Martelli
  • Clotilde Lauro
  • Paolo Biglioli
  • Alessandra Magenta
  • Giulio Pompilio
  • Elena De Falco
  • Gianluca Ragone
  • Maria Grazia Iachininoto

Detail Information

Publications25

  1. ncbi request reprint Nicotine modulates the spontaneous synaptic activity in cultured embryonic rat spinal cord interneurons
    Sergio Fucile
    Istituto Pasteur, Fondazione Cenci Bolognetti and Dipartimento di Fisiologia Umana e Farmacologia, Universita La Sapienza, Rome, Italy
    J Neurosci Res 67:329-36. 2002
    ..Our results provide the first evidence of modulation of both excitatory and inhibitory neurotransmitter release in embryonic spinal cord interneurons by non-alpha7-containing nicotinic receptors...
  2. pmc Effects of cyclothiazide on GluR1/AMPA receptors
    Sergio Fucile
    Istituto Pasteur Fondazione Cenci Bolognetti, Dipartimento di Fisiologia Umana e Farmacologia, Centro di Eccellenza BEMM, Universita di Roma La Sapienza, Piazzale Aldo Moro 5, I00185 Rome, Italy
    Proc Natl Acad Sci U S A 103:2943-7. 2006
    ..We also present a kinetic model that predicts well all of the experimental findings of CTZ action. Finally, we suggest a protocol for standard cell treatment with CTZ to attain maximal efficacy of CTZ on GluR1 receptors...
  3. ncbi request reprint Ca2+ permeability through rat cloned alpha9-containing nicotinic acetylcholine receptors
    Sergio Fucile
    Istituto Pasteur Fondazione Cenci Bolognetti and Dipartimento di Fisiologia Umana e Farmacologia, Centro di Eccellenza Biologia and Medicina Molecolare, Università di Rome La Sapienza, Italy
    Cell Calcium 39:349-55. 2006
    ..The physiological role played by Ca(2+) entry through alpha9-containing nAChRs gated by ACh is discussed...
  4. pmc Ca2+ permeability of nicotinic acetylcholine receptors from rat dorsal root ganglion neurones
    Sergio Fucile
    Istituto Pasteur Fondazione Cenci Bolognetti and Dipartimento di Fisiologia Umana e Farmacologia, Centro di Eccellenza Biologia e Medicina Molecolare, Universita di Roma La Sapienza, Roma, Italy
    J Physiol 565:219-28. 2005
    ..Finally, we report that Ca2+ influx through nAChRs in adult DRG neurones negatively modulated the TRPV1-mediated responses, representing a possible mechanism underlying the analgesic properties of nicotinic agonists on sensory neurones...
  5. ncbi request reprint Nicotinic cholinergic stimulation promotes survival and reduces motility of cultured rat cerebellar granule cells
    S Fucile
    Istituto Pasteur Fondazione Cenci Bolognetti and Dipartimento di Fisiologia Umana e Farmacologia, Centro di Eccellenza Biologia e Medicina Molecolare, Universita di Roma La Sapienza, P le Aldo Moro 5, I 00185 Roma, Italy
    Neuroscience 127:53-61. 2004
    ....
  6. ncbi request reprint Ca2+ permeability of nicotinic acetylcholine receptors
    Sergio Fucile
    Dipartimento di Fisiologia Umana e Farmacologia, Universita di Roma La Sapienza, P le Aldo Moro 5, I 00185 Rome, Italy
    Cell Calcium 35:1-8. 2004
    ..Different experimental approaches are compared, and the structural determinants of Ca(2+) permeability are discussed...
  7. ncbi request reprint Fractional Ca(2+) current through human neuronal alpha7 nicotinic acetylcholine receptors
    Sergio Fucile
    Istituto di Fisiologia Umana, Universita di Roma La Sapienza, P le Aldo Moro 5, I 00185 Rome, Italy
    Cell Calcium 34:205-9. 2003
    ..In our knowledge, the findings here reported indicate the human alpha7 receptors are the most Ca(2+) conductive homomeric ligand-gated receptor-channels expressed in a heterologous cell system...
  8. ncbi request reprint Chemokine receptor CXCR2 regulates the functional properties of AMPA-type glutamate receptor GluR1 in HEK cells
    P Lax
    Dipartimento di Fisiologia Umana e Farmacologia, Universita di Roma, Piazzale Aldo Moro 5, I00185, Rome, Italy
    J Neuroimmunol 129:66-73. 2002
    ..Our findings indicate that the coupling of CXCR2 with GluR1 may modulate glutamatergic synaptic transmission...
  9. ncbi request reprint Ca(2+) permeability of human heteromeric nAChRs expressed by transfection in human cells
    P Lax
    Dipartimento di Fisiologia Umana e Farmacologia, Istituto Pasteur Fondazione Cenci Bolognetti, Universita di Roma La Sapienza, P le Aldo Moro 5, I 00185 Roma, Italy
    Cell Calcium 32:53-8. 2002
    ..Thus, our findings are consistent with those elsewhere reported even using different experimental procedures, giving a strong support to the following sequence of Ca(2+) permeability: h-alpha 3 beta 4>h-alpha 4 beta 2>h-alpha 4 beta 4...
  10. ncbi request reprint Serotonin antagonizes the human neuronal alpha7 nicotinic acetylcholine receptor and becomes an agonist after L248T alpha7 mutation
    S Fucile
    Istituto Pasteur Fondazione Cenci Bolognetti, Dipartimento di Fisiologia Umana e Farmacologia, Universita di Roma La Sapienza, Viale Regina Elena 324, I 00161 Rome, Italy
    Neuroscience 110:169-79. 2002
    ....
  11. ncbi request reprint Functional properties of neurons derived from fetal mouse neurospheres are compatible with those of neuronal precursors in vivo
    Francesca Pagani
    Istituto Pasteur Fondazione Cenci Bolognetti, Dipartimento di Fisiologia Umana e Farmacologia and Centro di Eccellenza BEMM, Universita di Roma La Sapienza, Roma, Italy
    J Neurosci Res 83:1494-501. 2006
    ..Therefore, at least for a limited number of passages in vitro, neurospheres provide an adequate model of in vivo neurogenesis...
  12. pmc Acute and chronic effects of hypercalcaemia on cortical excitability as studied by 5 Hz repetitive transcranial magnetic stimulation
    Elisa Iacovelli
    Department of Neurological Sciences, Sapienza University of Rome, Viale dell Universita 30, 00185 Rome, Italy
    J Physiol 589:1619-26. 2011
    ....
  13. pmc Adenosine receptor antagonists alter the stability of human epileptic GABAA receptors
    Cristina Roseti
    Istituto Pasteur Fondazione Cenci Bolognetti and Dipartimento di Fisiologia Umana e Farmacologia, Centro di Eccellenza di Biologia e Medicina Molecolare, Universita di Roma La Sapienza, Piazzale A Moro 5, I 00185 Rome, Italy
    Proc Natl Acad Sci U S A 105:15118-23. 2008
    ..Our findings strongly suggest that cortical A2-A3 receptors alter the stability of GABA(A) receptors, which could offer therapeutic opportunities...
  14. pmc GABA(A)-current rundown of temporal lobe epilepsy is associated with repetitive activation of GABA(A) "phasic" receptors
    Eleonora Palma
    Dipartimento di Fisiologia Umana e Farmacologia Centro di Eccellenza Biologia e Medicina Molecolare, Universita di Roma Sapienza, Piazzale A Moro 5, I00185 Rome, Italy
    Proc Natl Acad Sci U S A 104:20944-8. 2007
    ..Our findings point to the rundown of GABA(A) receptors as a hallmark of TLE and suggest that modulating tonic and phasic mTLE GABA(A) receptor activity may represent a useful therapeutic approach to the disease...
  15. ncbi request reprint Cysteine residues are critical for chemokine receptor CXCR2 functional properties
    Cristina Limatola
    Istituto Pasteur Fondazione Cenci Bolognetti and Dipartimento di Fisiologia Umana e Farmacologia, Centro di Eccellenza BEMM, Universita La Sapienza, I 00185 Roma, Italy
    Exp Cell Res 307:65-75. 2005
    ....
  16. pmc Blockage of A2A and A3 adenosine receptors decreases the desensitization of human GABA(A) receptors microtransplanted to Xenopus oocytes
    Cristina Roseti
    Istituto Pasteur Fondazione Cenci Bolognetti and Dipartimento di Fisiologia Umana e Farmacologia Centro di Eccellenza BEMM, Universita di Roma La Sapienza, I00185 Rome, Italy
    Proc Natl Acad Sci U S A 106:15927-31. 2009
    ....
  17. pmc The single-channel properties of human acetylcholine alpha 7 receptors are altered by fusing alpha 7 to the green fluorescent protein
    Sergio Fucile
    Istituto Pasteur Fondazione Cenci Bolognetti and Dipartimento di Fisiologia Umana e Farmacologia, Universita di Roma La Sapienza, P le A Moro 5, I00185 Rome, Italy
    Proc Natl Acad Sci U S A 99:3956-61. 2002
    ..All this evidence shows that GFP-tagging can alter importantly receptor kinetics, a fact that has to be taken into account whenever tagged proteins are used to study their function...
  18. doi request reprint Mechanism of verapamil action on wild-type and slow-channel mutant human muscle acetylcholine receptor
    Claudia Moriconi
    Dipartimento di Fisiologia e Farmacologia, Universita La Sapienza, Roma, Italy
    J Neurochem 114:1231-40. 2010
    ....
  19. pmc Microtransplantation of membranes from cultured cells to Xenopus oocytes: a method to study neurotransmitter receptors embedded in native lipids
    Eleonora Palma
    Istituto Pasteur Fondazione Cenci Bolognetti and Dipartimento di Fisiologia Umana e Farmacologia, Universita di Roma La Sapienza, Piazzale Aldo Moro 5, I 00185 Rome, Italy
    Proc Natl Acad Sci U S A 100:2896-900. 2003
    ..All this makes the Xenopus oocyte a powerful tool for detailed investigations of receptors and other proteins expressed in the membrane of cultured cells...
  20. doi request reprint Rare missense variants of neuronal nicotinic acetylcholine receptor altering receptor function are associated with sporadic amyotrophic lateral sclerosis
    Mario Sabatelli
    Istituto di Neurologia, Universita Cattolica del Sacro Cuore, Roma, Italy
    Hum Mol Genet 18:3997-4006. 2009
    ....
  21. ncbi request reprint GABAergic drugs become neurotoxic in cortical neurons pre-exposed to brain-derived neurotrophic factor
    Gemma Molinaro
    Istituto Neurologico Mediterraneo Neuromed, 86077 Pozzilli, Italy
    Mol Cell Neurosci 37:312-22. 2008
    ..Our data raise the concern that GABAergic drugs may become neurotoxic under conditions associated with increases in brain BDNF levels...
  22. doi request reprint Functional properties of cells obtained from human cord blood CD34+ stem cells and mouse cardiac myocytes in coculture
    Alessia Orlandi
    Laboratorio di Biologia Vascolare e Terapia Genica, Centro Cardiologico Monzino IRCCS, Via Parea 4, 20138 Milan, Italy
    Am J Physiol Heart Circ Physiol 294:H1541-9. 2008
    ..However, the expression of human-specific cardiac genes was undetectable by RT-PCR...
  23. pmc The human adult subtype ACh receptor channel has high Ca2+ permeability and predisposes to endplate Ca2+ overloading
    Sergio Fucile
    Pasteur Institute Cenci Bolognetti Foundation and Department of Human Physiology and Pharmacology and Centre of Excellence for Biology and Molecular Medicine, University of Rome La Sapienza, Piazzale Aldo Moro 5 I 00185 Rome, Italy
    J Physiol 573:35-43. 2006
    ..However, the intrinsically high Ca2+ permeability of human AChRs probably predisposes to development of the endplate myopathy when opening events of the AChR channel are prolonged by altered AChR-channel kinetics...
  24. ncbi request reprint Electrophysiological properties of mouse bone marrow c-kit+ cells co-cultured onto neonatal cardiac myocytes
    Laura Lagostena
    Laboratorio di Biologia Vascolare e Terapia Genica, Centro Cardiologico Monzino, Istituto di Ricovero e Cura a Carattere Scientifico, Milan, Italy
    Cardiovasc Res 66:482-92. 2005
    ..In fact, some reports have shown the ability of stem cells from the bone marrow (BM) to differentiate into cardiac myocytes and to contribute to myocardium repair, while others have reported the opposite...
  25. ncbi request reprint Cyclin D1 degradation enhances endothelial cell survival upon oxidative stress
    Pasquale Fasanaro
    Istituto Dermopatico dell Immacolata IRCCS, Via dei Monti di Creta 104, Rome 00167, Italy
    FASEB J 20:1242-4. 2006
    ..Thus, cyclin D1 degradation enhances endothelial cell survival on oxidative stress...