Isabella Panfoli

Summary

Affiliation: University of Genoa
Location: Genova, Italy
Summary:
Permanent position as Senior Researcher-Aggregate Professor in Biochemistry, DIPTERIS-Faculty of Sciences MFN, University of Genoa, Italy.
Specific Skills: protein analysis, immunohystochemistry for mycrsoscopy; Luminometric, Fluorometric and Spectrophotometric enzymatic activity and kinetic analyses of proteins.
Principal Research topic: Bioenergetics in nervous system: extramitochondrial aerobic metabolism and TCA cycle, implications in retinal and neuronal diseases.

Publications

  1. doi request reprint Evidence for aerobic ATP synthesis in isolated myelin vesicles
    Silvia Ravera
    Biology Department, University of Genova, Faculty of Sciences MFN, Viale Benedetto XV, Genoa, Italy
    Int J Biochem Cell Biol 41:1581-91. 2009
  2. ncbi request reprint Heavy metal interference with growth hormone signalling in trout hepatoma cells RTH-149
    Barbara Marchi
    Dipartimento di Biologia, Universita di Genova, Viale Benedetto XV 5, 16132, Genova, Italy
    Biometals 18:179-90. 2005
  3. ncbi request reprint Efects of growth hormone and cadmium on the transcription regulation of two metallothionein isoforms
    Laura Vergani
    Department of Biophysical Sciences and Technologies M and O DISTBIMO, University of Genoa, Largo Rosanna Benzi 10, 16132 Genova, Italy
    Mol Cell Endocrinol 263:29-37. 2007
  4. doi request reprint Structural modification of proteins by direct electric current from low voltage
    Daniela Calzia
    Department of Biology, Universita di Genova, 16132 Genova, Italy
    J Biochem Mol Toxicol 23:309-17. 2009
  5. ncbi request reprint Simultaneous detection of molecular weight and activity of adenylate kinases after electrophoretic separation
    Silvia Ravera
    Department of Biology, University of Genoa, Genova, Italy
    Electrophoresis 28:291-300. 2007
  6. ncbi request reprint Expression of adenylate kinase 1 in bovine retinal cytosol
    Silvia Ravera
    Biology Department, University of Genoa, Genova, Italy
    Curr Eye Res 32:249-57. 2007
  7. doi request reprint The nuclear genes Mtfr1 and Dufd1 regulate mitochondrial dynamic and cellular respiration
    Massimiliano Monticone
    Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy
    J Cell Physiol 225:767-76. 2010
  8. doi request reprint Live imaging of mammalian retina: rod outer segments are stained by conventional mitochondrial dyes
    Paolo Bianchini
    University of Genoa, Laboratory for Advanced, Microscopy, Bioimaging, and Spectroscopy LAMBS, MicroSCoBiO Research Center, Department of Physics, Via Dodecaneso 33, 16146, Genoa, Italy
    J Biomed Opt 13:054017. 2008
  9. doi request reprint Why do premature newborn infants display elevated blood adenosine levels?
    Isabella Panfoli
    Dipartimento di Farmacia, Laboratorio di Biochimica, Universita di Genova, Genoa, Italy Electronic address
    Med Hypotheses 90:53-6. 2016
  10. doi request reprint Functional expression of oxidative phosphorylation proteins in the rod outer segment disc
    Isabella Panfoli
    DIFAR Biochemistry Lab, University of Genoa, 16132 Genova, Italy
    Cell Biochem Funct 31:532-8. 2013

Collaborators

Detail Information

Publications24

  1. doi request reprint Evidence for aerobic ATP synthesis in isolated myelin vesicles
    Silvia Ravera
    Biology Department, University of Genova, Faculty of Sciences MFN, Viale Benedetto XV, Genoa, Italy
    Int J Biochem Cell Biol 41:1581-91. 2009
    ..The complex of data suggests that myelin sheath may be the site of oxygen absorption and aerobic metabolism for the axons...
  2. ncbi request reprint Heavy metal interference with growth hormone signalling in trout hepatoma cells RTH-149
    Barbara Marchi
    Dipartimento di Biologia, Universita di Genova, Viale Benedetto XV 5, 16132, Genova, Italy
    Biometals 18:179-90. 2005
    ....
  3. ncbi request reprint Efects of growth hormone and cadmium on the transcription regulation of two metallothionein isoforms
    Laura Vergani
    Department of Biophysical Sciences and Technologies M and O DISTBIMO, University of Genoa, Largo Rosanna Benzi 10, 16132 Genova, Italy
    Mol Cell Endocrinol 263:29-37. 2007
    ..The synergy could depend on interactions in different signaling pathways, leading to a differential recruitment of MTF-1 and AP-1 transcription factors...
  4. doi request reprint Structural modification of proteins by direct electric current from low voltage
    Daniela Calzia
    Department of Biology, Universita di Genova, 16132 Genova, Italy
    J Biochem Mol Toxicol 23:309-17. 2009
    ..Enzymatic activity maybe a more accurate sensor of conformational changes than circular dichroism spectroscopy. A better understanding of efficacy of many electrical devices utilized in medical practice may follow...
  5. ncbi request reprint Simultaneous detection of molecular weight and activity of adenylate kinases after electrophoretic separation
    Silvia Ravera
    Department of Biology, University of Genoa, Genova, Italy
    Electrophoresis 28:291-300. 2007
    ..The procedure is also quantitative, allowing to determine the amount of enzyme present in samples...
  6. ncbi request reprint Expression of adenylate kinase 1 in bovine retinal cytosol
    Silvia Ravera
    Biology Department, University of Genoa, Genova, Italy
    Curr Eye Res 32:249-57. 2007
    ..In this study, we found that cytosolic adenylate kinase isoform 1 is expressed in bovine retina. Cytoplasmic AK1 would physiologically contribute to retinal energy metabolism...
  7. doi request reprint The nuclear genes Mtfr1 and Dufd1 regulate mitochondrial dynamic and cellular respiration
    Massimiliano Monticone
    Istituto Nazionale per la Ricerca sul Cancro, Genova, Italy
    J Cell Physiol 225:767-76. 2010
    ..These data show that, in murine testis, Dufd1 and Mtfr1 have redundant functions related to mitochondrial physiology and represent genes with a potential role in testicular function...
  8. doi request reprint Live imaging of mammalian retina: rod outer segments are stained by conventional mitochondrial dyes
    Paolo Bianchini
    University of Genoa, Laboratory for Advanced, Microscopy, Bioimaging, and Spectroscopy LAMBS, MicroSCoBiO Research Center, Department of Physics, Via Dodecaneso 33, 16146, Genoa, Italy
    J Biomed Opt 13:054017. 2008
    ..This finding may shed light on previous reports of the existence of a proton potential across the disk membranes and on the mechanism of the adenosine tri-phosphate (ATP) supply for phototransduction, which still requires investigation...
  9. doi request reprint Why do premature newborn infants display elevated blood adenosine levels?
    Isabella Panfoli
    Dipartimento di Farmacia, Laboratorio di Biochimica, Universita di Genova, Genoa, Italy Electronic address
    Med Hypotheses 90:53-6. 2016
    ..Adenosine may become a biomarker of prematurity risk, whose implications further studies may assess. ..
  10. doi request reprint Functional expression of oxidative phosphorylation proteins in the rod outer segment disc
    Isabella Panfoli
    DIFAR Biochemistry Lab, University of Genoa, 16132 Genova, Italy
    Cell Biochem Funct 31:532-8. 2013
    ..The existence of oxygen utilization in the outer retina, likely supplying ATP for phototransduction, may shed light on some retinal pathologies related to oxidative stress of the outer retina...
  11. doi request reprint A blue dive: from 'blue fingers' to 'blue silver'. A comparative overview of staining methods for in-gel proteomics
    Isabella Panfoli
    Dipartimento di Farmacia, Universita di Genova, Viale Bendetto XV, 3, 16132 Genova, Italy
    Expert Rev Proteomics 9:627-34. 2012
    ..An example of a 2-DE of human urine treated with combinatorial peptide ligand libraries demonstrates that implemented 'blue silver' can evidence the complexity of the sample...
  12. doi request reprint Extra-mitochondrial aerobic metabolism in retinal rod outer segments: new perspectives in retinopathies
    I Panfoli
    DIPTERIS University of Genova, Corso Europa 26, 16132 Genova, Italy
    Med Hypotheses 78:423-7. 2012
    ....
  13. pmc Inhibition of hemorragic snake venom components: old and new approaches
    Isabella Panfoli
    Department of Biology, University of Genova, Viale Benedetto XV 3, 16132 Genova, Italy
    Toxins (Basel) 2:417-27. 2010
    ..This mini-review summarizes new achievements in venom key component inhibition. A deeper knowledge of alternative ways to inhibit venom toxins may provide supplemental treatments to serum therapy...
  14. doi request reprint Evidence for aerobic metabolism in retinal rod outer segment disks
    Isabella Panfoli
    DIBIO, University of Genoa, Viale Benedetto XV, 5, 16132 Genova, Italy
    Int J Biochem Cell Biol 41:2555-65. 2009
    ..These findings may provide important new insight into energy production in outer segments via aerobic metabolism and additional information about protein components in OS disk membranes...
  15. doi request reprint 'Proteomineering': has the mine been excavated?
    Isabella Panfoli
    DIPTERIS Biochemistry Lab, University of Genoa, Genova, Italy
    Expert Rev Proteomics 8:443-5. 2011
    ..Therefore, the solid-phase ligand library made of hexapeptides remains a promising and unique tool for biomarker discovery...
  16. doi request reprint Extramitochondrial tricarboxylic acid cycle in retinal rod outer segments
    Isabella Panfoli
    Department of Biology, University of Genoa, Viale Benedetto XV, 5, 16132 Genova, Italy
    Biochimie 93:1565-75. 2011
    ..Data, suggestive of the exportability of the mitochondrial machinery for aerobic metabolism, may shed light on those retinal pathologies related to energy supply impairment in OS and to mutations in TCA enzymes...
  17. ncbi request reprint Accelerated removal of deamidated proteins and endogenous electric fields: possible implications
    Isabella Panfoli
    Department of Biology, University of Genova, 16132 Genova, Italy
    Gen Physiol Biophys 29:302-8. 2010
    ..Nano-local endogenous electric fields arise within cells. Endogenous currents may cause the unfolding of the products of deamidation at Asn. In turn, these unfolded proteins would be removed, likely by proteolysis...
  18. doi request reprint Immunochemical or fluorescent labeling of vesicular subcellular fractions for microscopy imaging
    Isabella Panfoli
    Department of Biology, University of Genoa, Italy
    Microsc Res Tech 73:1086-90. 2010
    ..Therefore, the technique seems adequate to become an instrument to study the structure and the function of these and other subcellular fractions...
  19. doi request reprint Proteomic analysis of the retinal rod outer segment disks
    Isabella Panfoli
    Department of Biology, University of Genoa, V le Benedetto XV 3, 16132 Genova, Italy
    J Proteome Res 7:2654-69. 2008
    ..This study represents a step toward a global physiological characterization of the disk proteome and provides information necessary for future studies on energy supply for phototransduction...
  20. ncbi request reprint Inactivation of phospholipase A2 and metalloproteinase from Crotalus atrox venom by direct current
    Isabella Panfoli
    Department of Biology, University of Genoa, V le Bendetto XV, 3, 16132 Genova, Italy
    J Biochem Mol Toxicol 21:7-12. 2007
    ....
  21. ncbi request reprint Localization of the cyclic ADP-ribose-dependent calcium signaling pathway in bovine rod outer segments
    Isabella Panfoli
    Department of Biology, University of Genoa, Genova, Italy
    Invest Ophthalmol Vis Sci 48:978-84. 2007
    ..In this study, the presence and functional role of the adenosine diphosphoribosyl (ADPR)-cyclase-cyclic ADP-ribose (cADPR) system in bovine retinal rod outer segments (ROS) was investigated...
  22. ncbi request reprint Essential role of Ca2+ -dependent phospholipase A2 in estradiol-induced lysosome activation
    Bruno Burlando
    Dipartimento di Scienze e Tecnologie Avanzate, Universita del Piemonte Orientale Amedeo Avogadro, 15100 Alessandria, Biologia e Genetica, Universita di Genova, 16132 Genoa, Italy
    Am J Physiol Cell Physiol 283:C1461-8. 2002
    ..This mechanism promotes lysosome fusion and catabolic activities and may mediate short-term estradiol effects...
  23. ncbi request reprint Ligand-independent tyrosine kinase signalling in RTH 149 trout hepatoma cells: comparison among heavy metals and pro-oxidants
    Bruno Burlando
    Dipartimento di Scienze e Tecnologie Avanzate, Universita del Piemonte Orientale Amedeo Avogadro, Corso Borsalino 54, 15100 Alessandria, Italy
    Cell Physiol Biochem 13:147-54. 2003
    ..Phosphospecific STAT antibodies revealed activation by H(2)O(2) only. In conclusion, fish cell exposure to heavy metals and pro-oxidants produce specific tyrosine kinase responses, involving cross talk and redox modulatory effects...
  24. ncbi request reprint Confocal laser scanning microscopy of retinal rod outer segment intact disks: new labeling technique
    Silvia Ravera
    J Biomed Opt 12:050501. 2007
    ..In fact, even though much is known about the rod outer segment photoresponse, some unanswered questions remain, particularly about ATP supply, light adaptation, and morphogenesis...