Mirko Pinotti

Summary

Affiliation: University of Ferrara
Country: Italy

Publications

  1. doi request reprint Rescue of coagulation factor VII function by the U1+5A snRNA
    Mirko Pinotti
    Department of Biochemistry, University of Ferrara, Italy
    Blood 113:6461-4. 2009
  2. doi request reprint Ribosome readthrough accounts for secreted full-length factor IX in hemophilia B patients with nonsense mutations
    Mirko Pinotti
    Dipartimento di Biochimica e Biologia Molecolare and LTTA Centre, Universita di Ferrara, Italy
    Hum Mutat 33:1373-6. 2012
  3. ncbi request reprint Daily and circadian rhythms of tissue factor pathway inhibitor and factor VII activity
    Mirko Pinotti
    Deparment of di Biochimica e Biologia Molecolare, Universita di Ferrara, Via L Borsari 46, 44100 Ferrara, Italia
    Arterioscler Thromb Vasc Biol 25:646-9. 2005
  4. pmc Evidence for an overlapping role of CLOCK and NPAS2 transcription factors in liver circadian oscillators
    Cristiano Bertolucci
    Dipartimento di Biochimica e Biologia Molecolare, Universita di Ferrara, Via Fossato di Mortara 74, 44100 Ferrara, Italia
    Mol Cell Biol 28:3070-5. 2008
  5. pmc Activation of a cryptic splice site in a potentially lethal coagulation defect accounts for a functional protein variant
    Nicola Cavallari
    Department of Biochemistry and Molecular Biology, and LTTA, University of Ferrara, Italy
    Biochim Biophys Acta 1822:1109-13. 2012
  6. ncbi request reprint Characterization of mild coagulation factor VII deficiency: activity and clearance of the Arg315Trp and Arg315Lys variants in the Cys310-Cys329 loop (c170s)
    Christian Furlan Freguia
    Department of Biochemistry and Molecular Biology, University of Ferrara, Italy
    Haematologica 89:1504-9. 2004
  7. ncbi request reprint U1-snRNA-mediated rescue of mRNA processing in severe factor VII deficiency
    Mirko Pinotti
    Department of Biochemistry and Molecular Biology, University of Ferrara, Via Fossato di Mortara 74, Ferrara, Italy
    Blood 111:2681-4. 2008
  8. pmc Natural and engineered carboxy-terminal variants: decreased secretion and gain-of-function result in asymptomatic coagulation factor VII deficiency
    Alessio Branchini
    Dipartimento di Biochimica e Biologia Molecolare and LTTA Centre, Universita degli Studi di Ferrara, Ferrara, Italy
    Haematologica 97:705-9. 2012
  9. ncbi request reprint Circadian rhythms in mouse blood coagulation
    Cristiano Bertolucci
    Department of Biology and Neuroscience Centre, University of Ferrara, Ferrara, Italy
    J Biol Rhythms 20:219-24. 2005
  10. pmc Factor VII mutant V154G models a zymogen-like form of factor VIIa
    Raffaella Toso
    Department of Biochemistry and Molecular Biology, University of Ferrara, Via Luigi Borsari, 46 Ferrara 44100, Italy
    Biochem J 369:563-71. 2003

Collaborators

Detail Information

Publications28

  1. doi request reprint Rescue of coagulation factor VII function by the U1+5A snRNA
    Mirko Pinotti
    Department of Biochemistry, University of Ferrara, Italy
    Blood 113:6461-4. 2009
    ....
  2. doi request reprint Ribosome readthrough accounts for secreted full-length factor IX in hemophilia B patients with nonsense mutations
    Mirko Pinotti
    Dipartimento di Biochimica e Biologia Molecolare and LTTA Centre, Universita di Ferrara, Italy
    Hum Mutat 33:1373-6. 2012
    ..Data in patients' plasma and in vitro, obtained in the proper protein context, support a ribosome readthrough gradient, consistent with its predicted determinants of efficiency...
  3. ncbi request reprint Daily and circadian rhythms of tissue factor pathway inhibitor and factor VII activity
    Mirko Pinotti
    Deparment of di Biochimica e Biologia Molecolare, Universita di Ferrara, Via L Borsari 46, 44100 Ferrara, Italia
    Arterioscler Thromb Vasc Biol 25:646-9. 2005
    ..We sought for temporal fluctuations of tissue factor pathway inhibitor (TFPI) activity in human and mouse plasma...
  4. pmc Evidence for an overlapping role of CLOCK and NPAS2 transcription factors in liver circadian oscillators
    Cristiano Bertolucci
    Dipartimento di Biochimica e Biologia Molecolare, Universita di Ferrara, Via Fossato di Mortara 74, 44100 Ferrara, Italia
    Mol Cell Biol 28:3070-5. 2008
    ..Our data provide the first evidence in peripheral oscillators for an overlapping role of CLOCK and NPAS2 in the regulation of circadianly controlled genes...
  5. pmc Activation of a cryptic splice site in a potentially lethal coagulation defect accounts for a functional protein variant
    Nicola Cavallari
    Department of Biochemistry and Molecular Biology, and LTTA, University of Ferrara, Italy
    Biochim Biophys Acta 1822:1109-13. 2012
    ..In conclusion, we provide experimental clues for traces of FVII expression, which might have reverted an otherwise perinatally lethal genetic condition...
  6. ncbi request reprint Characterization of mild coagulation factor VII deficiency: activity and clearance of the Arg315Trp and Arg315Lys variants in the Cys310-Cys329 loop (c170s)
    Christian Furlan Freguia
    Department of Biochemistry and Molecular Biology, University of Ferrara, Italy
    Haematologica 89:1504-9. 2004
    ..Natural FVII variants at position 315 provide peculiar models for structure-function studies...
  7. ncbi request reprint U1-snRNA-mediated rescue of mRNA processing in severe factor VII deficiency
    Mirko Pinotti
    Department of Biochemistry and Molecular Biology, University of Ferrara, Via Fossato di Mortara 74, Ferrara, Italy
    Blood 111:2681-4. 2008
    ..These findings suggest compensatory U1-snRNAs as therapeutic tools in coagulation factor deficiencies caused by mutations at 5'ss, a frequent cause of severe defects...
  8. pmc Natural and engineered carboxy-terminal variants: decreased secretion and gain-of-function result in asymptomatic coagulation factor VII deficiency
    Alessio Branchini
    Dipartimento di Biochimica e Biologia Molecolare and LTTA Centre, Universita degli Studi di Ferrara, Ferrara, Italy
    Haematologica 97:705-9. 2012
    ..R462X mutation, which explains the asymptomatic FVII deficiency, and contributes to our understanding of the role of the highly variable carboxy-terminus of coagulation serine proteases...
  9. ncbi request reprint Circadian rhythms in mouse blood coagulation
    Cristiano Bertolucci
    Department of Biology and Neuroscience Centre, University of Ferrara, Ferrara, Italy
    J Biol Rhythms 20:219-24. 2005
    ..These findings help to elucidate the complex control on the coagulation process and might contribute in explaining the temporal variations in the frequency of cardiovascular events observed in humans...
  10. pmc Factor VII mutant V154G models a zymogen-like form of factor VIIa
    Raffaella Toso
    Department of Biochemistry and Molecular Biology, University of Ferrara, Via Luigi Borsari, 46 Ferrara 44100, Italy
    Biochem J 369:563-71. 2003
    ....
  11. doi request reprint Membrane binding and anticoagulant properties of protein S natural variants
    Marcello Baroni
    Department of Biochemistry and Molecular Biology, ICSI, University of Ferrara, Ferrara, Italy
    Thromb Res 125:e33-9. 2010
    ..We compared anticoagulant and membrane binding properties of PS altered by natural mutations (N217S, DelI203D204) affecting the epidermal growth factor like-domain 4 (EGF4) and causing PS deficiency...
  12. ncbi request reprint Temporal variations of coagulation factor VII activity in mice are influenced by lighting regime
    Ilaria Colognesi
    Department of Biology and Evolution and Neuroscience Centre, University of Ferrara, Ferrara, Italy
    Chronobiol Int 24:305-13. 2007
    ..Taken together, these findings in mice provide novel insights into the modulation of FVII activity levels, which might have implications for human pathophysiology...
  13. doi request reprint Coagulation factor VII variants resistant to inhibitory antibodies
    Alessio Branchini
    Prof Francesco Bernardi, Department of Life Sciences and Biotechnology, University of Ferrara, Via Fossato di Mortara 74, 44121 Ferrara, Italy, Tel 39 0532 974425, Fax 39 0532 974484, E mail
    Thromb Haemost 112:972-80. 2014
    ..Taken together our results provide a paradigmatic example of mutation-related inhibitory antibodies, strongly support the FVII carboxyl-terminus as their main target and identify inhibitor-resistant FVII variants. ..
  14. pmc Intracellular evaluation of ER targeting elucidates a mild form of inherited coagulation deficiency
    Lara Rizzotto
    Department of Biochemistry and Molecular Biology, University of Ferrara, Via Fossato di Mortara 74, Ferrara, Italy
    Mol Med 12:137-42. 2006
    ..This approach, extendable to other coagulation serine proteases, clearly contributed to elucidate the relationship of genotype with plasma and clinical phenotype...
  15. ncbi request reprint Contribution of low density lipoprotein receptor-related protein genotypes to coagulation factor VIII levels in thrombotic women
    Giovanna Marchetti
    Department of Biochemistry and Molecular Biology, University of Ferrara, via Fossato di Mortara n 74, I 44100 Ferrara, Italy
    Haematologica 91:1261-3. 2006
    ....
  16. pmc A frequent human coagulation Factor VII mutation (A294V, c152) in loop 140s affects the interaction with activators, tissue factor and substrates
    Raffaella Toso
    Department of Biochemistry and Molecular Biology, University of Ferrara, Via Borsari, 46 Ferrara 44100, Italy
    Biochem J 363:411-6. 2002
    ....
  17. doi request reprint Characterization of the intracellular signalling capacity of natural FXa mutants with reduced pro-coagulant activity
    Monia Monti
    Department of Biochemistry and Molecular Biology, University of Ferrara, Italy
    Thromb Res 123:914-8. 2009
    ....
  18. pmc An Exon-Specific U1snRNA Induces a Robust Factor IX Activity in Mice Expressing Multiple Human FIX Splicing Mutants
    Dario Balestra
    Department of Life Sciences and Biotechnology, University of Ferrara, Ferrara, Italy
    Mol Ther Nucleic Acids 5:e370. 2016
    ....
  19. doi request reprint Functional genetics
    Giovanna Marchetti
    Department of Biochemistry and Molecular Biology, University of Ferrara, Ferrara, Italy
    Thromb Res 129:336-40. 2012
    ..Taking into account our previous and current experimental activities we shall focus on a few examples and open issues in cardiovascular disorders, the main clinical topic of this short review...
  20. pmc Replacement of the Y450 (c234) phenyl ring in the carboxyl-terminal region of coagulation factor IX causes pleiotropic effects on secretion and enzyme activity
    Alessio Branchini
    Department of Life Sciences and Biotechnology and LTTA Center, University of Ferrara, Ferrara, Italy
    FEBS Lett 587:3249-53. 2013
    ....
  21. doi request reprint Long-chain cationic derivatives of PTA (1,3,5-triaza-7-phosphaadamantane) as new components of potential non-viral vectors
    Rita Cortesi
    Department of Pharmaceutical Sciences, University of Ferrara, Ferrara, Italy
    Int J Pharm 431:176-82. 2012
    ..These data, and particularly the ability of CP-SLN to protect DNA from degradation, encourages further studies aimed at proposing these nanosystems as a potential approach to deliver nucleic acid to cells in living organisms...
  22. ncbi request reprint Characterization of anti-coagulant properties of prenylated coumarin ferulenol
    Monia Monti
    Dipartimento di Biochimica e Biologia Molecolare, Universita di Ferrara, Italy
    Biochim Biophys Acta 1770:1437-40. 2007
    ..Studies with ferulenol derivatives indicated the prenyl residue as major determinant of ferulenol activity...
  23. ncbi request reprint Stimulation of P2 (P2X7) receptors in human dendritic cells induces the release of tissue factor-bearing microparticles
    Marcello Baroni
    Department of Biochemistry, University of Ferrara, Ferrara, Italy
    FASEB J 21:1926-33. 2007
    ..These findings might have implications for triggering and propagating coagulation in healthy and atherosclerotic vessels...
  24. doi request reprint Exploring Splicing-Switching Molecules For Seckel Syndrome Therapy
    Daniela Scalet
    Department of Life Sciences and Biotechnology, Section of Biochemistry and Molecular Biology, University of Ferrara, Via Fossato di Mortara 74, 44121 Ferrara, Italy
    Biochim Biophys Acta 1863:15-20. 2017
    ..Altogether these data elucidate the molecular mechanisms of the ATR c.2101A>G mutation and identify two potential complementary RNA-based therapies for Seckel syndrome...
  25. ncbi request reprint Asymptomatic carriership of factor V Leiden and genotypes of the fibrinogen gene cluster
    Giovanna Marchetti
    Dipartimento di Biochimica e Biologia Molecolare, Universita di Ferrara, Unità di Ricerca Clinica sulla Trombofilia Marino Golinelli, Divisione di Angiologia, Azienda Ospedaliera di Bologna, Policlinico S Orsola Malpighi, Bologna, Italia
    Br J Haematol 121:632-8. 2003
    ..35 vs 2.69 +/- 0.41 g/l, P = 0.028) among the asymptomatic subjects. Our data suggest that, in the presence of inherited thrombophilia, frequent fibrinogen polymorphisms may interact to modulate the risk of venous thromboembolism...
  26. pmc An engineered tale-transcription factor rescues transcription of factor VII impaired by promoter mutations and enhances its endogenous expression in hepatocytes
    Elena Barbon
    Department of Life Sciences and Biotechnology, University of Ferrara, Italy
    Sci Rep 6:28304. 2016
    ..These results provide experimental evidence for TALE-TFs as gene-specific tools useful to counteract disease-causing promoter mutations...
  27. doi request reprint Factor XIII-A dynamics in acute myocardial infarction: a novel prognostic biomarker?
    Donato Gemmati
    Gemmati Donato, Ctr Hemostasis and Thrombosis, Hematology Section, Dpt of Medical Sciences, University of Ferrara, Ferrara, Italy, Tel 39 0532 237291, Fax 39 0532 209010, E mail
    Thromb Haemost 114:123-32. 2015
    ..Different FXIII-A dynamics and levels could be utilised as early prognostic indicators during acute MI, revealing the individual potential to heal and suggesting tailored treatments to avoid heart failure or its extreme consequence...
  28. ncbi request reprint Residual factor VII activity and different hemorrhagic phenotypes in CRM(+) factor VII deficiencies (Gly331Ser and Gly283Ser)
    Mirko Pinotti
    Dipartimento di Biochimica e Biologia Molecolare CIBF, University of Ferrara, Italy
    Blood 99:1495-7. 2002
    ..Furthermore, in a plasma system with limiting thromboplastin concentration, 283Ser-FVII inhibited wild-type FVIIa activity in a dose-dependent manner...