Izuho Hatada

Summary

Affiliation: Gunma University
Country: Japan

Publications

  1. ncbi request reprint Imprinting of a small nucleolar RNA gene on mouse chromosome 12
    Masafumi Shimoda
    Gene Research Center, Gunma University, 3 39 22 Showa machi, Maebashi, Gunma, 371 8511, Japan
    Genomics 79:483-6. 2002
  2. pmc Astrocyte-specific genes are generally demethylated in neural precursor cells prior to astrocytic differentiation
    Izuho Hatada
    Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Japan
    PLoS ONE 3:e3189. 2008
  3. ncbi request reprint Emerging technologies for genome-wide DNA methylation profiling in cancer
    Izuho Hatada
    Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi 371 8511 Japan
    Crit Rev Oncog 12:205-23. 2006
  4. ncbi request reprint Genome-wide profiling of promoter methylation in human
    I Hatada
    Laboratory of Genome Science, Biosignal Genome Resource Center, Department of Molecular and Cellular Biology, Gunma University, Maebashi, Japan
    Oncogene 25:3059-64. 2006
  5. doi request reprint Genome-wide demethylation during neural differentiation of P19 embryonal carcinoma cells
    Izuho Hatada
    Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, 3 39 15 Showa Machi, Maebashi 371 8512, Japan
    J Hum Genet 53:185-91. 2008
  6. ncbi request reprint A microarray-based method for detecting methylated loci
    Izuho Hatada
    Gene Research Center, Gunma University, 3 39 22 Showa machi, Maebashi 371 8511, Japan
    J Hum Genet 47:448-51. 2002
  7. ncbi request reprint Identification of a new imprinted gene, Rian, on mouse chromosome 12 by fluorescent differential display screening
    I Hatada
    Gene Research Center, Gunma University, Showa machi, Maebashi, Gunma 371 8511, Japan
    J Biochem 130:187-90. 2001
  8. ncbi request reprint Microarray analysis of promoter methylation in lung cancers
    Masayuki Fukasawa
    Laboratory of Genome Science, Biosignal Genome Resource Center, Department of Molecular and Cellular Biology, Gunma University, 3 39 15 Showa Machi, Maebashi 371 8512, Japan
    J Hum Genet 51:368-74. 2006
  9. doi request reprint Epigenetic differences between embryonic stem cells generated from blastocysts developed in vitro and in vivo
    Takuro Horii
    Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, Japan
    Cell Reprogram 12:551-63. 2010
  10. pmc Epigenetic regulation of adipocyte differentiation by a Rho guanine nucleotide exchange factor, WGEF
    Takuro Horii
    Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Japan
    PLoS ONE 4:e5809. 2009

Collaborators

Detail Information

Publications35

  1. ncbi request reprint Imprinting of a small nucleolar RNA gene on mouse chromosome 12
    Masafumi Shimoda
    Gene Research Center, Gunma University, 3 39 22 Showa machi, Maebashi, Gunma, 371 8511, Japan
    Genomics 79:483-6. 2002
    ....
  2. pmc Astrocyte-specific genes are generally demethylated in neural precursor cells prior to astrocytic differentiation
    Izuho Hatada
    Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Japan
    PLoS ONE 3:e3189. 2008
    ..Thus, late-stage NPCs have epigenetic potential which can be realized in their expression after astrocyte differentiation...
  3. ncbi request reprint Emerging technologies for genome-wide DNA methylation profiling in cancer
    Izuho Hatada
    Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi 371 8511 Japan
    Crit Rev Oncog 12:205-23. 2006
    ..Several technologies are emerging for the genome-wide profiling of DNA methylation to find new tumor-suppressor genes and predictions for clinical phenotypes in individual patients...
  4. ncbi request reprint Genome-wide profiling of promoter methylation in human
    I Hatada
    Laboratory of Genome Science, Biosignal Genome Resource Center, Department of Molecular and Cellular Biology, Gunma University, Maebashi, Japan
    Oncogene 25:3059-64. 2006
    ..Genome-wide analysis of hypomethylated promoter sequences in cancer demonstrated low CG/GC ratio of these sequences, suggesting that CpG-poor genes are sensitive to demethylation activity in cancer...
  5. doi request reprint Genome-wide demethylation during neural differentiation of P19 embryonal carcinoma cells
    Izuho Hatada
    Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, 3 39 15 Showa Machi, Maebashi 371 8512, Japan
    J Hum Genet 53:185-91. 2008
    ..We found a genome-wide demethylation of genes. This suggests demethylation rather than methylation is important in neural differentiation...
  6. ncbi request reprint A microarray-based method for detecting methylated loci
    Izuho Hatada
    Gene Research Center, Gunma University, 3 39 22 Showa machi, Maebashi 371 8511, Japan
    J Hum Genet 47:448-51. 2002
    ..Choosing the methylated DNA library as the resource material of the microarray increased the percentage of DNA fragments derived from hypermethylated loci on the microarray...
  7. ncbi request reprint Identification of a new imprinted gene, Rian, on mouse chromosome 12 by fluorescent differential display screening
    I Hatada
    Gene Research Center, Gunma University, Showa machi, Maebashi, Gunma 371 8511, Japan
    J Biochem 130:187-90. 2001
    ..The Rian transcript did not have any apparent open reading frame, and its transcript was exclusively localized to the nucleus...
  8. ncbi request reprint Microarray analysis of promoter methylation in lung cancers
    Masayuki Fukasawa
    Laboratory of Genome Science, Biosignal Genome Resource Center, Department of Molecular and Cellular Biology, Gunma University, 3 39 15 Showa Machi, Maebashi 371 8512, Japan
    J Hum Genet 51:368-74. 2006
    ..This demonstrates that our PMAD method is effective at finding epigenetic changes during cancer...
  9. doi request reprint Epigenetic differences between embryonic stem cells generated from blastocysts developed in vitro and in vivo
    Takuro Horii
    Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, Japan
    Cell Reprogram 12:551-63. 2010
    ..In conclusion, it is advisable to use early passage Vivo ES cells whenever feasible, or to select ES cell lines with a normal epigenotype...
  10. pmc Epigenetic regulation of adipocyte differentiation by a Rho guanine nucleotide exchange factor, WGEF
    Takuro Horii
    Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Japan
    PLoS ONE 4:e5809. 2009
    ..This study clarified that adipogenesis was regulated by WGEF expression through DNA methylation change...
  11. pmc miR-29 represses the activities of DNA methyltransferases and DNA demethylases
    Sumiyo Morita
    Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, Gunma 371 8512, Japan
    Int J Mol Sci 14:14647-58. 2013
    ....
  12. ncbi request reprint The Dnmt3b splice variant is specifically expressed in in vitro-manipulated blastocysts and their derivative ES cells
    Takuro Horii
    Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, Gunma 371 8512, Japan
    J Reprod Dev 57:579-85. 2011
    ..In vitro methylation activity assays showed that Dnmt3bΔ6 had lower activity than normal Dnmt3b. Our findings suggest that Dnmt3bΔ6 could induce a hypomethylation status especially in in vitro manipulated embryos...
  13. ncbi request reprint One Argonaute family member, Eif2c2 (Ago2), is essential for development and appears not to be involved in DNA methylation
    Sumiyo Morita
    Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, 3 39 15 Showa Machi, Maebashi 371 8512, Japan
    Genomics 89:687-96. 2007
    ..This suggests that developmental defects in the Eif2c2-deficient mouse are caused not at the transcriptional level, but rather at the posttranscriptional level through the miRNA-protein complex...
  14. pmc Genome-wide analysis of DNA methylation and expression of microRNAs in breast cancer cells
    Sumiyo Morita
    Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, Gunma 371 8512, Japan E Mails S M T H M K
    Int J Mol Sci 13:8259-72. 2012
    ..This is only rarely observed in other gene categories, including miRNAs. The present study highlights the epigenetic similarities and differences between miRNA and protein-coding genes...
  15. ncbi request reprint Loss of genomic imprinting in mouse parthenogenetic embryonic stem cells
    Takuro Horii
    Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, Gunma, Japan
    Stem Cells 26:79-88. 2008
    ..On the other hand, in germ lineages, both PGES and PG in chimeras showed normal erasure of imprints, indicating that the reprogramming in germ lineages is an inevitable event, regardless of the imprint status of primordial germ cells...
  16. doi request reprint Targeted DNA demethylation in vivo using dCas9-peptide repeat and scFv-TET1 catalytic domain fusions
    Sumiyo Morita
    Institute for Molecular and Cellular Regulation, Gunma University, Maebashi, Japan
    Nat Biotechnol 34:1060-1065. 2016
    ..7- to 50-fold upregulation of associated genes both in cell culture (embryonic stem cells, cancer cell lines, primary neural precursor cells) and in vivo in mouse fetuses...
  17. pmc Gene expression profiling of white adipose tissue reveals paternal transmission of proneness to obesity
    Sumiyo Morita
    Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, 3 39 15 Showa machi Maebashi, 371 8512, Japan
    Sci Rep 6:21693. 2016
    ..In addition, using single-nucleotide polymorphisms that differ between B6 and PWK, we identified candidate imprinted genes in WAT. ..
  18. doi request reprint MeCP2-dependent repression of an imprinted miR-184 released by depolarization
    Tasuku Nomura
    Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, 3 39 15 Showa Machi, Maebashi 371 8512, Japan
    Hum Mol Genet 17:1192-9. 2008
    ..The restricted release of MeCP2 from the paternal allele results in paternal allele-specific expression of miR-184. Our finding provides a clue to the link between the microRNA and DNA methylation pathways...
  19. ncbi request reprint Gene silencing: maturation of mouse fetal germ cells in vitro
    Yayoi Obata
    Gene Research Center, Gunma University, 3 39 22 Showa machi, Maebashi, Gunma 371 8511, Japan
    Nature 418:497. 2002
    ..To our knowledge, this is the first time that complete oogenesis has been successfully accomplished in vitro...
  20. pmc p53 suppresses tetraploid development in mice
    Takuro Horii
    Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, 3 39 15 Showa Machi, Maebashi, Gunma 371 8512, Japan
    Sci Rep 5:8907. 2015
    ..Finally, we found that p53 downregulation rescued tetraploid embryos later in gestation. ..
  21. pmc Dicer is required for maintaining adult pancreas
    Sumiyo Morita
    Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, Showa Machi Maebashi, Japan
    PLoS ONE 4:e4212. 2009
    ..This suggested that Dicer1 is important for maintaining the adult pancreas...
  22. doi request reprint Validation of microinjection methods for generating knockout mice by CRISPR/Cas-mediated genome engineering
    Takuro Horii
    1 Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, 3 39 15 Showa Machi, Maebashi, Gunma 371 8512, Japan 2
    Sci Rep 4:4513. 2014
    ..We found that injection of RNA into the cytoplasm was the most efficient method in terms of the numbers of viable blastocyst stage embryos and full-term pups generated. This method also showed the best overall knockout efficiency. ..
  23. pmc Generation of an ICF syndrome model by efficient genome editing of human induced pluripotent stem cells using the CRISPR system
    Takuro Horii
    Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, Gunma 371 8512, Japan
    Int J Mol Sci 14:19774-81. 2013
    ..We obtained iPS cells with mutations in both alleles of DNA methyltransferase 3B (DNMT3B) in 63% of transfected clones. Our data suggest that the CRISPR system is highly efficient and useful for genome engineering of human iPS cells. ..
  24. ncbi request reprint Genomic imprinting and Beckwith-Wiedemann syndrome
    I Hatada
    Gene Research Center, Gunma University, Maebashi, Japan
    Histol Histopathol 15:309-12. 2000
    ..Beckwith-Wiedemann syndrome (BWS), a congenital overgrowth syndrome with increased risk of childhood tumors, is one of the well-known diseases caused by imprinted genes. The imprinted genes causing BWS are discussed in this review...
  25. pmc Paternal allele influences high fat diet-induced obesity
    Sumiyo Morita
    Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, Showa Machi Maebashi, Japan
    PLoS ONE 9:e85477. 2014
    ..Taken together, our findings suggest that the down-regulation of Igf2 and Peg3 imprinted genes in adipocytes may be involved in the paternal transmission of HFD-induced obesity. ..
  26. pmc Regulation of CpG methylation by Dnmt and Tet in pluripotent stem cells
    Takuro Horii
    Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, Gunma 371 8512, Japan
    J Reprod Dev 62:331-5. 2016
    ..Here, we discuss CpG methylation instability and the regulation of CpG methylation by DNA methyltransferases and ten-eleven translocation in pluripotent stem cells. ..
  27. doi request reprint Production of genome-edited pluripotent stem cells and mice by CRISPR/Cas
    Takuro Horii
    Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, Gunma, Japan
    Endocr J 63:213-9. 2016
    ..In this review, we provide an overview of genome editing by CRISPR/Cas in pluripotent stem cells and mice. ..
  28. doi request reprint Genome editing: A breakthrough in life science and medicine
    Izuho Hatada
    Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, Maebashi 371 8512, Japan
    Endocr J 63:105-10. 2016
    ..Genome editing simplifies and accelerates the creation of animal disease models and enables construction of models in most animal species, even those that are not amenable to conventional gene targeting technology. ..
  29. pmc Challenges to increasing targeting efficiency in genome engineering
    Takuro Horii
    Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, Gunma 371 8512, Japan
    J Reprod Dev 62:7-9. 2016
    ..in this issue. Here, we outline current gene targeting technologies with special emphasis on HR-mediated technologies, which are currently being performed using these two major strategies. ..
  30. pmc Genome Editing Using Mammalian Haploid Cells
    Takuro Horii
    Laboratory of Genome Science, Biosignal Genome Resource Center, Institute for Molecular and Cellular Regulation, Gunma University, 3 39 15 Showa Machi, Maebashi, Gunma 371 8512, Japan
    Int J Mol Sci 16:23604-14. 2015
    ..For example, CRISPR/Cas was used in haploid ESCs to generate multiple knockouts and large deletions at high efficiency. In addition, genome-wide screening is facilitated by haploid cell lines containing gene knockout libraries. ..
  31. ncbi request reprint Genomic imprinting in Dicer1-hypomorphic mice
    M Fukasawa
    Biosignal Genome Resource Center, Department of Molecular and Cellular Biology, Gunma University, Maebashi, Japan
    Cytogenet Genome Res 113:138-43. 2006
    ..We did not observe any abnormality in the allelic expression of imprinted genes in these mice or their offspring, suggesting that reduced expression of Dicer1 did not significantly affect the maintenance and reprogramming of imprinting...
  32. ncbi request reprint Characterization and imprinting status of OBPH1/Obph1 gene: implications for an extended imprinting domain in human and mouse
    Ken Higashimoto
    Department of Biomolecular Sciences, Saga Medical School, 5 1 1 Nabeshima, Saga, 849 8501, Japan
    Genomics 80:575-84. 2002
    ..It remains to be elucidated whether the gene is under the control of the KIP2/LIT1 subdomain or is regulated by a specific mechanism. Analysis of the precise genomic sequence around the region should help resolve this question...
  33. doi request reprint A comparative analysis of the transcriptome and signal pathways in hepatic differentiation of human adipose mesenchymal stem cells
    Yusuke Yamamoto
    Section for Studies on Metastasis, National Cancer Center Research Institute, 1 1 Tsukiji 5 chome, Chuo Ku, Tokyo, Japan
    FEBS J 275:1260-73. 2008
    ....
  34. pmc Domain regulation of imprinting cluster in Kip2/Lit1 subdomain on mouse chromosome 7F4/F5: large-scale DNA methylation analysis reveals that DMR-Lit1 is a putative imprinting control region
    Hitomi Yatsuki
    Department of Biochemistry, Saga Medical School, Saga, Saga 849 8501, Japan
    Genome Res 12:1860-70. 2002
    ..These data indicate that CGI8, called DMR-Lit1, is not only the region for gametic methylation but might also be the imprinting control region (ICR) of the subdomain...
  35. ncbi request reprint Unregulated expression of the imprinted genes H19 and Igf2r in mouse uniparental fetuses
    Yusuke Sotomaru
    Department of Animal Science, Tokyo University of Agriculture, 1737, Funako, Atsugi, Kanagawa 243 0034, Japan
    J Biol Chem 277:12474-8. 2002
    ....