Mito Kanatsu-Shinohara

Summary

Affiliation: Kyoto University
Country: Japan

Publications

  1. doi request reprint Nonrandom Germline Transmission of Mouse Spermatogonial Stem Cells
    Mito Kanatsu-Shinohara
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Yoshida Konoe, Sakyo ku, Kyoto 606 8501, Japan Japan Science and Technology Agency, PRESTO, Kyoto 606 8501, Japan Electronic address
    Dev Cell 38:248-61. 2016
  2. pmc Skp1-Cullin-F-box (SCF)-type ubiquitin ligase FBXW7 negatively regulates spermatogonial stem cell self-renewal
    Mito Kanatsu-Shinohara
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan Precursory Research for Embryonic Science and Technology PRESTO and
    Proc Natl Acad Sci U S A 111:8826-31. 2014
  3. doi request reprint Spermatogonial stem cell self-renewal and development
    Mito Kanatsu-Shinohara
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan Email
    Annu Rev Cell Dev Biol 29:163-87. 2013
  4. doi request reprint Reconstitution of mouse spermatogonial stem cell niches in culture
    Mito Kanatsu-Shinohara
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Cell Stem Cell 11:567-78. 2012
  5. doi request reprint Homologous recombination in rat germline stem cells
    Mito Kanatsu-Shinohara
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Biol Reprod 85:208-17. 2011
  6. pmc Transmission distortion by loss of p21 or p27 cyclin-dependent kinase inhibitors following competitive spermatogonial transplantation
    Mito Kanatsu-Shinohara
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Proc Natl Acad Sci U S A 107:6210-5. 2010
  7. doi request reprint Homing of mouse spermatogonial stem cells to germline niche depends on beta1-integrin
    Mito Kanatsu-Shinohara
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Cell Stem Cell 3:533-42. 2008
  8. doi request reprint Pluripotency of a single spermatogonial stem cell in mice
    Mito Kanatsu-Shinohara
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Biol Reprod 78:681-7. 2008
  9. ncbi request reprint Clonal origin of germ cell colonies after spermatogonial transplantation in mice
    Mito Kanatsu-Shinohara
    Horizontal Medical Research Organization, Department of Molecular Genetics, Kyoto University, Japan
    Biol Reprod 75:68-74. 2006
  10. ncbi request reprint Anchorage-independent growth of mouse male germline stem cells in vitro
    Mito Kanatsu-Shinohara
    Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Kyoto 606 8507, Japan
    Biol Reprod 74:522-9. 2006

Detail Information

Publications67

  1. doi request reprint Nonrandom Germline Transmission of Mouse Spermatogonial Stem Cells
    Mito Kanatsu-Shinohara
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Yoshida Konoe, Sakyo ku, Kyoto 606 8501, Japan Japan Science and Technology Agency, PRESTO, Kyoto 606 8501, Japan Electronic address
    Dev Cell 38:248-61. 2016
    ..Thus, spermatogenesis is a regulated process whereby specific SSC clones are repeatedly recruited for fertilization with long-term cycles. ..
  2. pmc Skp1-Cullin-F-box (SCF)-type ubiquitin ligase FBXW7 negatively regulates spermatogonial stem cell self-renewal
    Mito Kanatsu-Shinohara
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan Precursory Research for Embryonic Science and Technology PRESTO and
    Proc Natl Acad Sci U S A 111:8826-31. 2014
    ..These results suggest that FBXW7 regulates SSC self-renewal in a negative manner by degradation of MYC. ..
  3. doi request reprint Spermatogonial stem cell self-renewal and development
    Mito Kanatsu-Shinohara
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan Email
    Annu Rev Cell Dev Biol 29:163-87. 2013
    ..An improved understanding of SSC self-renewal through functional assays promises to uncover fundamental principles of stem cell biology and will enable us to use these cells for applications in animal transgenesis and medicine. ..
  4. doi request reprint Reconstitution of mouse spermatogonial stem cell niches in culture
    Mito Kanatsu-Shinohara
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Cell Stem Cell 11:567-78. 2012
    ..Moreover, GDNF upregulated CXCL12 receptor expression, and CXCL12 transfection in Sertoli cells increased homing efficiency. Overall, our findings identify GDNF and CXCL12 as SSC chemotactic factors in vitro and in vivo...
  5. doi request reprint Homologous recombination in rat germline stem cells
    Mito Kanatsu-Shinohara
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Biol Reprod 85:208-17. 2011
    ..Nevertheless, our results demonstrate the feasibility of gene targeting in rat GS cells and pave the way toward the generation of KO rats...
  6. pmc Transmission distortion by loss of p21 or p27 cyclin-dependent kinase inhibitors following competitive spermatogonial transplantation
    Mito Kanatsu-Shinohara
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Proc Natl Acad Sci U S A 107:6210-5. 2010
    ....
  7. doi request reprint Homing of mouse spermatogonial stem cells to germline niche depends on beta1-integrin
    Mito Kanatsu-Shinohara
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Cell Stem Cell 3:533-42. 2008
    ..These results identify beta1-integrin as an essential adhesion receptor for SSC homing and its association with laminin is critical in multiple steps of SSC homing...
  8. doi request reprint Pluripotency of a single spermatogonial stem cell in mice
    Mito Kanatsu-Shinohara
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Biol Reprod 78:681-7. 2008
    ..Thus, our study provides evidence that a single spermatogonial stem cell can acquire pluripotentiality but that conversion into a pluripotent cell type is accompanied by loss of spermatogenic potential...
  9. ncbi request reprint Clonal origin of germ cell colonies after spermatogonial transplantation in mice
    Mito Kanatsu-Shinohara
    Horizontal Medical Research Organization, Department of Molecular Genetics, Kyoto University, Japan
    Biol Reprod 75:68-74. 2006
    ..These results provide evidence that germ cell colonies develop from single spermatogonial stem cells...
  10. ncbi request reprint Anchorage-independent growth of mouse male germline stem cells in vitro
    Mito Kanatsu-Shinohara
    Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Kyoto 606 8507, Japan
    Biol Reprod 74:522-9. 2006
    ..These results indicate that spermatogonial stem cells can undergo anchorage-independent, self-renewal division, and suggest that stem cells have the common property to survive and proliferate in the absence of exogenous substrata...
  11. ncbi request reprint Production of knockout mice by gene targeting in multipotent germline stem cells
    Masanori Takehashi
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Yoshida Konoe, Sakyo ku, Kyoto 606 8501, Japan
    Dev Biol 312:344-52. 2007
    ..Thus, mGS cells can be used to produce KO animals, and this novel method of germline manipulation may prove useful in diverse mammalian species...
  12. doi request reprint Production of transgenic rats via lentiviral transduction and xenogeneic transplantation of spermatogonial stem cells
    Mito Kanatsu-Shinohara
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Biol Reprod 79:1121-8. 2008
    ..Thus, xenogeneic transplantation is a powerful strategy for transgenesis, and smaller xenogeneic surrogates can be used for male germline modification using SSCs...
  13. pmc Production of knockout mice by random or targeted mutagenesis in spermatogonial stem cells
    Mito Kanatsu-Shinohara
    Department of Molecular Genetics, Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan tshinoha2virus kyoto u ac jp
    Proc Natl Acad Sci U S A 103:8018-23. 2006
    ..These results demonstrate the feasibility of altering genes in tissue-specific stem cells in a manner similar to embryonic stem cells and have important implications for gene therapy and animal transgenesis...
  14. ncbi request reprint Long-term proliferation in culture and germline transmission of mouse male germline stem cells
    Mito Kanatsu-Shinohara
    Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Japan
    Biol Reprod 69:612-6. 2003
    ..Long-term spermatogonial stem cell culture will be useful for studying spermatogenesis mechanism and has important implications for developing new technology in transgenesis or medicine...
  15. pmc Bone marrow engraftment but limited expansion of hematopoietic cells from multipotent germline stem cells derived from neonatal mouse testis
    Momoko Yoshimoto
    Department of Pediatrics, Kyoto University, Kyoto
    Exp Hematol 37:1400-10. 2009
    ..In the present work, we examined mGS cells for hematopoietic progenitor potential in vitro and in vivo...
  16. ncbi request reprint Genetic and epigenetic properties of mouse male germline stem cells during long-term culture
    Mito Kanatsu-Shinohara
    Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Development 132:4155-63. 2005
    ....
  17. ncbi request reprint Long-term culture of male germline stem cells from hamster testes
    Mito Kanatsu-Shinohara
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Biol Reprod 78:611-7. 2008
    ....
  18. pmc Phenotypic plasticity of mouse spermatogonial stem cells
    Hiroko Morimoto
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    PLoS ONE 4:e7909. 2009
    ....
  19. ncbi request reprint Spermatogenesis from epiblast and primordial germ cells following transplantation into postnatal mouse testis
    Shinichiro Chuma
    Department of Development and Differentiation, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    Development 132:117-22. 2005
    ..Primordial germ cell transplantation techniques will also provide a novel tool to assess the developmental potential of PGCs, such as those manipulated in vitro or recovered from embryos harboring lethal mutations...
  20. pmc Adenovirus-mediated gene delivery into mouse spermatogonial stem cells
    Masanori Takehashi
    Department of Molecular Genetics, Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Proc Natl Acad Sci U S A 104:2596-601. 2007
    ..Nevertheless, these results suggest that adenovirus may inadvertently integrate into the patient's germ line and indicate that there is no barrier to adenovirus infection in spermatogonial stem cells...
  21. ncbi request reprint Long-term culture of mouse male germline stem cells under serum-or feeder-free conditions
    Mito Kanatsu-Shinohara
    Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Biol Reprod 72:985-91. 2005
    ..This culture system will provide a new opportunity for understanding the regulatory mechanism that governs spermatogonial stem cells...
  22. ncbi request reprint Genetic influences in mouse spermatogonial stem cell self-renewal
    Mito Kanatsu-Shinohara
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Japan
    J Reprod Dev 56:145-53. 2010
    ..Furthermore, application of genetic modification techniques to GS cells with non-DBA/2 backgrounds extends the potential of a SSC-based approach in male germline modification...
  23. ncbi request reprint Leukemia inhibitory factor enhances formation of germ cell colonies in neonatal mouse testis culture
    Mito Kanatsu-Shinohara
    Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Biol Reprod 76:55-62. 2007
    ..These results demonstrate that LIF is useful in the initiation of GS cell culture and suggest that LIF or a related cytokine is involved in the maturation of gonocytes into spermatogonia...
  24. ncbi request reprint Generation of pluripotent stem cells from neonatal mouse testis
    Mito Kanatsu-Shinohara
    Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Cell 119:1001-12. 2004
    ..The ability to derive multipotential stem cells from the neonatal testis has important implications for germ cell biology and opens the possibility of using these cells for biotechnology and medicine...
  25. pmc Rats produced by interspecies spermatogonial transplantation in mice and in vitro microinsemination
    Takashi Shinohara
    Department of Molecular Genetics and Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Proc Natl Acad Sci U S A 103:13624-8. 2006
    ..The xenogeneic offspring production by interspecies germ cell transplantation and in vitro microinsemination will become a powerful tool in animal transgenesis and species conservation...
  26. doi request reprint Neonatal mouse testis-derived multipotent germline stem cells improve the cardiac function of acute ischemic heart mouse model
    Toru Iwasa
    Department of Pediatrics, Graduate School of Medicine, Kyoto University, 54 Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Biochem Biophys Res Commun 400:27-33. 2010
    ..In conclusion, Flk1(+) mGS cell transplantation can improve the cardiac function of ischemic hearts by promoting angiogenesis and by delaying host cell death via senescence...
  27. doi request reprint Heritable imprinting defect caused by epigenetic abnormalities in mouse spermatogonial stem cells
    Jiyoung Lee
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Sakyo ku, Kyoto, Japan
    Biol Reprod 80:518-27. 2009
    ..These results indicate that fetal germ cells have a limited ability to become pluripotent cells and lose the ability to undergo epigenetic reprogramming by in vitro culture...
  28. doi request reprint In vitro transformation of mouse testis cells by oncogene transfection
    Hiroko Morimoto
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Biol Reprod 86:148, 1-11. 2012
    ..Thus, in vitro testis cell transformation facilitates an analysis of the GCT formation process, and our results also suggest the close similarity between GCT formation and reprogramming...
  29. ncbi request reprint Akt mediates self-renewal division of mouse spermatogonial stem cells
    Jiyoung Lee
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Development 134:1853-9. 2007
    ..These results demonstrate that activation of the PI3K-Akt pathway plays a central role in the self-renewal division of spermatogonial stem cells...
  30. doi request reprint Rac mediates mouse spermatogonial stem cell homing to germline niches by regulating transmigration through the blood-testis barrier
    Seiji Takashima
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Cell Stem Cell 9:463-75. 2011
    ..Short hairpin RNA (shRNA) -mediated suppression of claudin3, which was downregulated by Rac inhibition, reduced the SSC homing efficiency. Thus, Rac1 is a critical regulator of SSC homing and proliferation...
  31. doi request reprint Hybridization of testis-derived stem cells with somatic cells and embryonic stem cells in mice
    Masanori Takehashi
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Biol Reprod 86:178. 2012
    ..These data show that mGS cells possess ES-like reprogramming potential, which predominates over-SSC activity...
  32. pmc Dynamic changes in EPCAM expression during spermatogonial stem cell differentiation in the mouse testis
    Mito Kanatsu-Shinohara
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    PLoS ONE 6:e23663. 2011
    ..We examined the correlation between their expression patterns and SSC activities...
  33. ncbi request reprint Transgenic mice produced by retroviral transduction of male germ line stem cells in vivo
    Mito Kanatsu-Shinohara
    Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University Yoshida Konoe, Kyoto, Japan
    Biol Reprod 71:1202-7. 2004
    ....
  34. ncbi request reprint Allogeneic offspring produced by male germ line stem cell transplantation into infertile mouse testis
    Mito Kanatsu-Shinohara
    Department of Medical Chemistry, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Biol Reprod 68:167-73. 2003
    ..Thus, these results indicate that a histoincompatible recipient can serve as a "surrogate father" to propagate the genetic information of heterologous male donors...
  35. pmc Regulation of pluripotency in male germline stem cells by Dmrt1
    Seiji Takashima
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Genes Dev 27:1949-58. 2013
    ..These results suggest that spontaneous SSC reprogramming is caused by unstable DNA methylation and that a Dmrt1-Sox2 cascade is critical for regulating pluripotency in SSCs. ..
  36. doi request reprint Genetic reconstruction of mouse spermatogonial stem cell self-renewal in vitro by Ras-cyclin D2 activation
    Jiyoung Lee
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Cell Stem Cell 5:76-86. 2009
    ..Thus, the Ras-cyclin D2 pathway regulates the balance between tissue maintenance and tumorigenesis in the SSC population...
  37. ncbi request reprint Regulation of mouse spermatogonial stem cell self-renewing division by the pituitary gland
    Mito Kanatsu-Shinohara
    Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Japan
    Biol Reprod 70:1731-7. 2004
    ..Thus, the pituitary gland affects the self-renewing division of stem cells, but these contradictory results suggest that its role may be different depending on the stage of the testicular development...
  38. ncbi request reprint Functional assessment of self-renewal activity of male germline stem cells following cytotoxic damage and serial transplantation
    Mito Kanatsu-Shinohara
    Department of Medical Chemistry, Graduate School of Medicine, Kyoto University, Japan
    Biol Reprod 68:1801-7. 2003
    ..8- and 12-fold within 2 and 4 mo, respectively. These results provide direct functional evidence for the expansion of stem cells and establish the basis for further characterization of the stem cell self-renewal process...
  39. ncbi request reprint Generation of cardiac and endothelial cells from neonatal mouse testis-derived multipotent germline stem cells
    Shiro Baba
    Department of Pediatrics, Graduate School of Medicine, Kyoto University, 54 Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8507, Japan
    Stem Cells 25:1375-83. 2007
    ..Disclosure of potential conflicts of interest is found at the end of this article...
  40. doi request reprint Enrichment of mouse spermatogonial stem cells by melanoma cell adhesion molecule expression
    Mito Kanatsu-Shinohara
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Biol Reprod 87:139. 2012
    ..Development of a new strategy for SSC enrichment from mature adult testes will facilitate analyses of SSCs in the normal testicular microenvironment...
  41. pmc Adenovirus-mediated gene delivery and in vitro microinsemination produce offspring from infertile male mice
    Mito Kanatsu-Shinohara
    Department of Medical Chemistry, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Proc Natl Acad Sci U S A 99:1383-8. 2002
    ....
  42. doi request reprint Serum- and feeder-free culture of mouse germline stem cells
    Mito Kanatsu-Shinohara
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Biol Reprod 84:97-105. 2011
    ..This culture system provides a basic platform for understanding the regulation of SSC fate commitment in vitro and for improving SSC culture medium...
  43. doi request reprint Abnormal DNA methyltransferase expression in mouse germline stem cells results in spermatogenic defects
    Seiji Takashima
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Biol Reprod 81:155-64. 2009
    ..Although genetic manipulation did not increase the conversion of GS cells to a pluripotent state, these results underscore the important role of DNMTs in survival and spermatogenic differentiation in SSCs...
  44. ncbi request reprint Genetic selection of mouse male germline stem cells in vitro: offspring from single stem cells
    Mito Kanatsu-Shinohara
    Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Biol Reprod 72:236-40. 2005
    ....
  45. pmc Transcriptional repression and DNA hypermethylation of a small set of ES cell marker genes in male germline stem cells
    Masanori Imamura
    Department of Stem Cell Biology, Institute for Frontier Medical Sciences, Kyoto University, Kyoto 606 8507, Japan
    BMC Dev Biol 6:34. 2006
    ..We previously identified a set of genes called ECATs (ES cell-associated transcripts) that are expressed at high levels in mouse ES cells. Here, we examine the expression and DNA methylation of ECATs in somatic cells and germ cells...
  46. doi request reprint Enrichment of Mouse Spermatogonial Stem Cells by the Stem Cell Dye CDy1
    Mito Kanatsu-Shinohara
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto, Japan Japan Science and Technology Agency, PRESTO, Kyoto, Japan
    Biol Reprod 94:13. 2016
    ..Thus, CDy1 staining will provide a useful strategy for the enrichment of SSCs and may be used in conjunction with other reagents for the enrichment of SSCs. ..
  47. pmc Functional differences between GDNF-dependent and FGF2-dependent mouse spermatogonial stem cell self-renewal
    Seiji Takashima
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Stem Cell Reports 4:489-502. 2015
    ..Our results show that SSCs exhibit at least two modes of self-renewal and suggest complexity of SSC regulation in vivo...
  48. doi request reprint FGF2 mediates mouse spermatogonial stem cell self-renewal via upregulation of Etv5 and Bcl6b through MAP2K1 activation
    Kei Ishii
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Development 139:1734-43. 2012
    ..These results show that FGF2 depends on MAP2K1 signaling to drive SSC self-renewal via upregulation of the Etv5 and Bcl6b genes...
  49. ncbi request reprint Culture and genetic modification of mouse germline stem cells
    Mito Kanatsu-Shinohara
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Yoshida Konoe, Sakyo, Kyoto 606 8501, Japan
    Ann N Y Acad Sci 1120:59-71. 2007
    ..These new spermatogonial stem-cell lines will be useful for studying the mechanism of spermatogenesis, and they have important implications for developing new transgenic or medical technologies...
  50. ncbi request reprint Direct visualization of transplanted hematopoietic cell reconstitution in intact mouse organs indicates the presence of a niche
    Momoko Yoshimoto
    Department of Pediatrics, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Exp Hematol 31:733-40. 2003
    ..Our goal is to examine in vivo reconstitution processes and candidate niches in all bones in the mouse body using a new visualization method...
  51. doi request reprint Germline modification using mouse spermatogonial stem cells
    Mito Kanatsu-Shinohara
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Methods Enzymol 477:17-36. 2010
    ..The genetic and epigenetic stability of SSCs provide new possibilities for the application of germline mutagenesis in a wide range of animals...
  52. ncbi request reprint CD9 is a surface marker on mouse and rat male germline stem cells
    Mito Kanatsu-Shinohara
    Horizontal Medical Research Organization, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Biol Reprod 70:70-5. 2004
    ....
  53. pmc Myc/Mycn-mediated glycolysis enhances mouse spermatogonial stem cell self-renewal
    Mito Kanatsu-Shinohara
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Genes Dev 30:2637-2648. 2016
    ..These results suggested that Myc-mediated glycolysis is an important factor that increases the frequency of SSC self-renewal division...
  54. doi request reprint ROS are required for mouse spermatogonial stem cell self-renewal
    Hiroko Morimoto
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Cell Stem Cell 12:774-86. 2013
    ..These results suggest that ROS generated by Nox1 play critical roles in SSC self-renewal via the activation of the p38 MAPK and JNK pathways...
  55. ncbi request reprint Unstable side population phenotype of mouse spermatogonial stem cells in vitro
    Takashi Shinohara
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    J Reprod Dev 57:288-95. 2011
    ..These results show that SSCs have an unstable SP phenotype and provide evidence that SSCs change their phenotype characteristics in response to their microenvironment...
  56. pmc Pluripotent cell derivation from male germline cells by suppression of Dmrt1 and Trp53
    Takashi Tanaka
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    J Reprod Dev 61:473-84. 2015
    ..These results show that PGCs and gonocytes/spermatogonia have distinct reprogramming potential and also suggest that fresh and cultured SSCs do not necessarily have the same properties. ..
  57. doi request reprint Improved serum- and feeder-free culture of mouse germline stem cells
    Mito Kanatsu-Shinohara
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto, Japan Japan Science and Technology Agency, PRESTO, Kyoto, Japan
    Biol Reprod 91:88. 2014
    ....
  58. pmc The CDKN1B-RB1-E2F1 pathway protects mouse spermatogonial stem cells from genomic damage
    Takashi Tanaka
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    J Reprod Dev 61:305-16. 2015
    ..These results suggest that the CDKN1B-RB1-E2F1 pathway is essential for SSC self-renewal and protects SSCs against genomic damage. ..
  59. pmc The Trp53-Trp53inp1-Tnfrsf10b pathway regulates the radiation response of mouse spermatogonial stem cells
    Kei Ishii
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan Electronic address
    Stem Cell Reports 3:676-89. 2014
    ..These results suggest that the Trp53-Trp53inp1-Tnfrsf10b pathway responds to genotoxic damage in SSCs and that stem and progenitor cells exhibit distinct DNA damage responses in self-renewing tissue. ..
  60. ncbi request reprint Association of microRNA-34a overexpression with proliferation is cell type-dependent
    Khokon Kumar Dutta
    Department of Pathology and Biology of Diseases, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Cancer Sci 98:1845-52. 2007
    ....
  61. ncbi request reprint [Male germline stem cells: stem cells for germline modification and regenerative medicine]
    Takashi Shinohara
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, 53 Kawahara cho, Shogoin, Sakyo ku, Kyoto 606 8501, Japan
    Seikagaku 77:1409-12. 2005
  62. pmc The Luteinizing Hormone-Testosterone Pathway Regulates Mouse Spermatogonial Stem Cell Self-Renewal by Suppressing WNT5A Expression in Sertoli Cells
    Takashi Tanaka
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    Stem Cell Reports 7:279-91. 2016
    ..WNT5A promoted SSC activity both in vitro and in vivo. Therefore, FSH is not responsible for GDNF regulation, while LH negatively regulates SSC self-renewal by suppressing WNT5A via testosterone. ..
  63. doi request reprint Fertility of Male Germline Stem Cells Following Spermatogonial Transplantation in Infertile Mouse Models
    Mito Kanatsu-Shinohara
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto, Japan Japan Science and Technology Agency, PRESTO, Kyoto, Japan
    Biol Reprod 94:112. 2016
    ..Efficient offspring production using GS cells and spermatogonial transplantation will be useful for analyzing factors involved in male fertility. ..
  64. doi request reprint ROS-Generating Oxidase Nox3 Regulates the Self-Renewal of Mouse Spermatogonial Stem Cells
    Hiroko Morimoto
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Biol Reprod 92:147. 2015
    ..Our results suggest that self-renewal of SSCs is regulated by sequential activation of different Nox genes, and underscore the complexity of ROS regulation in the self-renewal division of SSCs. ..
  65. pmc Cell-cycle-dependent colonization of mouse spermatogonial stem cells after transplantation into seminiferous tubules
    Kei Ishii
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto 606 8501, Japan
    J Reprod Dev 60:37-46. 2014
    ..Thus cell cycle status is an important factor in regulating SSC migration to the niche. ..
  66. pmc p53-dependent S-phase damage checkpoint and pronuclear cross talk in mouse zygotes with X-irradiated sperm
    Tsutomu Shimura
    Department of Late Effect Studies, Radiation Biology Center, Kyoto University, Yoshida Konoe, Sakyo ku, Kyoto 606 8501, Japan
    Mol Cell Biol 22:2220-8. 2002
    ..These results revealed the presence of p53-dependent pronuclear cross talk and a novel function of p53 in the S-phase DNA damage checkpoint of mouse zygotes...
  67. doi request reprint Enrichment of mouse spermatogonial stem cells based on aldehyde dehydrogenase activity
    Mito Kanatsu-Shinohara
    Department of Molecular Genetics, Graduate School of Medicine, Kyoto University, Kyoto, Japan
    Biol Reprod 89:140. 2013
    ..Development of a new technique for SSC isolation from wild-type testes based on their functional properties will facilitate investigation of SSCs in a normal testicular environment. ..