Xuenan Xuan

Summary

Affiliation: Obihiro University of Agriculture and Veterinary Medicine
Country: Japan

Publications

  1. pmc Overproduction of Toxoplasma gondii cyclophilin-18 regulates host cell migration and enhances parasite dissemination in a CCR5-independent manner
    Hany M Ibrahim
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    BMC Microbiol 14:76. 2014
  2. pmc The Babesia bovis gene and promoter model: an update from full-length EST analysis
    Junya Yamagishi
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho west 2 13, Obihiro, Hokkaido 080 8555, Japan
    BMC Genomics 15:678. 2014
  3. pmc Cloning and characterization of a 2-Cys peroxiredoxin from Babesia gibsoni
    Tatsunori Masatani
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    J Vet Med Sci 76:139-43. 2014
  4. pmc Detection of antibodies to Babesia equi in horses by a latex agglutination test using recombinant EMA-1
    X Xuan
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    Clin Diagn Lab Immunol 8:645-6. 2001
  5. ncbi request reprint Detection of antibodies to Neospora caninum in cattle by enzyme-linked immunosorbent assay with truncated NcSRS2 expressed in Escherichia coli
    Irungu Gaturaga
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    J Parasitol 91:191-2. 2005
  6. ncbi request reprint Identification and characterization of cross-reactive antigens from Neospora caninum and Toxoplasma gondii
    M Liao
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Parasitology 130:481-8. 2005
  7. ncbi request reprint Diagnosis of equine piroplasmosis in Xinjiang province of China by the enzyme-linked immunosorbent assays using recombinant antigens
    Xuenan Xuan
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Vet Parasitol 108:179-82. 2002
  8. pmc Multivariable analysis of host amino acids in plasma and liver during infection of malaria parasite Plasmodium yoelii
    Erisha Saiki
    Department of Tropical Medicine, The Jikei University School of Medicine, 3 25 8, Nishi Shinbashi, Tokyo 105 8461, Japan
    Malar J 12:19. 2013
  9. ncbi request reprint Babesia gibsoni ribosomal phosphoprotein P0 induces cross-protective immunity against B. microti infection in mice
    M Alaa Terkawi
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Vaccine 25:2027-35. 2007
  10. ncbi request reprint Molecular characterization of a gene encoding a 29-kDa cytoplasmic protein of Babesia gibsoni and evaluation of its diagnostic potentiality
    Shinya Fukumoto
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Mol Biochem Parasitol 131:129-36. 2003

Collaborators

Detail Information

Publications73

  1. pmc Overproduction of Toxoplasma gondii cyclophilin-18 regulates host cell migration and enhances parasite dissemination in a CCR5-independent manner
    Hany M Ibrahim
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    BMC Microbiol 14:76. 2014
    ..We examined the functional role of T. gondii cyclophilin 18 (TgCyp18) in host cell recruitment using recombinant parasites transfected with TgCyp18...
  2. pmc The Babesia bovis gene and promoter model: an update from full-length EST analysis
    Junya Yamagishi
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho west 2 13, Obihiro, Hokkaido 080 8555, Japan
    BMC Genomics 15:678. 2014
    ..Therefore, we validated a preproposed gene model of B. bovis and extended the associated annotations on the basis of experimentally obtained full-length expressed sequence tags (ESTs)...
  3. pmc Cloning and characterization of a 2-Cys peroxiredoxin from Babesia gibsoni
    Tatsunori Masatani
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    J Vet Med Sci 76:139-43. 2014
    ..BgTPx-1 was expressed in the cytoplasm of B. gibsoni merozoites. These results suggest that the BgTPx-1 may play a role to control redox balance in the cytoplasm of B. gibsoni. ..
  4. pmc Detection of antibodies to Babesia equi in horses by a latex agglutination test using recombinant EMA-1
    X Xuan
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    Clin Diagn Lab Immunol 8:645-6. 2001
    ..These results indicate that LAT using recombinant EMA-1 might be very useful as a routine screening method for the diagnosis of B. equi infection...
  5. ncbi request reprint Detection of antibodies to Neospora caninum in cattle by enzyme-linked immunosorbent assay with truncated NcSRS2 expressed in Escherichia coli
    Irungu Gaturaga
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    J Parasitol 91:191-2. 2005
    ..6%) were confirmed as positive by Western blot analysis with whole-parasite antigens. These results suggest that ELISA with recombinant NcSRS2t is an effective method for diagnosis of N. caninum infection in cattle...
  6. ncbi request reprint Identification and characterization of cross-reactive antigens from Neospora caninum and Toxoplasma gondii
    M Liao
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Parasitology 130:481-8. 2005
    ..Some of the proteins could be useful in developing vaccines or drugs for controlling the diseases caused by the two parasites...
  7. ncbi request reprint Diagnosis of equine piroplasmosis in Xinjiang province of China by the enzyme-linked immunosorbent assays using recombinant antigens
    Xuenan Xuan
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Vet Parasitol 108:179-82. 2002
    ..To our knowledge, this is the first report describing a survey on equine piroplasmosis in Xinjiang province, China...
  8. pmc Multivariable analysis of host amino acids in plasma and liver during infection of malaria parasite Plasmodium yoelii
    Erisha Saiki
    Department of Tropical Medicine, The Jikei University School of Medicine, 3 25 8, Nishi Shinbashi, Tokyo 105 8461, Japan
    Malar J 12:19. 2013
    ....
  9. ncbi request reprint Babesia gibsoni ribosomal phosphoprotein P0 induces cross-protective immunity against B. microti infection in mice
    M Alaa Terkawi
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Vaccine 25:2027-35. 2007
    ..microti challenge infection. These data suggest that BgP0 is a potentially universal vaccine candidate for both B. gibsoni and B. microti infections...
  10. ncbi request reprint Molecular characterization of a gene encoding a 29-kDa cytoplasmic protein of Babesia gibsoni and evaluation of its diagnostic potentiality
    Shinya Fukumoto
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Mol Biochem Parasitol 131:129-36. 2003
    ..gibsoni in dogs...
  11. ncbi request reprint Serodiagnosis of Neospora caninum infection in cattle by enzyme-linked immunosorbent assay with recombinant truncated NcSAG1
    Bayin Chahan
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Vet Parasitol 118:177-85. 2003
    ..These results suggest that the recombinant NcSAG1t could be a reliable reagent for use as an antigen in ELISA for the serodiagnosis of N. caninum infection in cattle...
  12. ncbi request reprint Identification of three protein disulfide isomerase members from Haemaphysalis longicornis tick
    Min Liao
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Insect Biochem Mol Biol 37:641-54. 2007
    ..0 and 4.0 times higher than uninfected normal larval ticks, respectively. The results indicate that HlPDI-1 and HlPDI-3 might be involved in tick blood feeding and Babesia parasite infection in ticks...
  13. ncbi request reprint Serodiagnosis of canine Babesia gibsoni infection by enzyme-linked immunosorbent assay with recombinant P50 expressed in Escherichia coli
    Shinya Fukumoto
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    J Parasitol 90:387-91. 2004
    ..These results indicate that the recombinant P50 expressed in E. coil is a useful diagnostic antigen for practical use in the diagnosis of B. gibsoni infection in dogs...
  14. doi request reprint Neospora caninum: application of apical membrane antigen 1 encapsulated in the oligomannose-coated liposomes for reduction of offspring mortality from infection in BALB/c mice
    Houshuang Zhang
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Exp Parasitol 125:130-6. 2010
    ..Thus, a parasite-specific Th1 immune response was successfully induced in the pregnant mice immunized with M3-NcAMA1, and an effective reduction of offspring mortality from N. caninum infection was triggered...
  15. pmc Identification of secreted antigen 3 from Babesia gibsoni
    Honglin Jia
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Clin Vaccine Immunol 16:944-8. 2009
    ..gibsoni. The identification of BgSA3 provided a useful target for the development of a diagnostic test for detecting specific antibodies and circulating antigens...
  16. doi request reprint Characterization of the Babesia gibsoni 12-kDa protein as a potential antigen for the serodiagnosis
    Youn Kyoung Goo
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    Parasitol Int 58:55-60. 2009
    ..gibsoni infection that could be used as a diagnostic antigen for B. gibsoni infection with high specificity and sensitivity...
  17. ncbi request reprint Identification of ribosomal phosphoprotein P0 of Neospora caninum as a potential common vaccine candidate for the control of both neosporosis and toxoplasmosis
    Houshuang Zhang
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Mol Biochem Parasitol 153:141-8. 2007
    ..caninum and T. gondii in vitro in a concentration-dependent manner. These results indicate that P0 is a cross-reactive antigen between N. caninum and T. gondii and a potential common vaccine candidate to control both parasites...
  18. ncbi request reprint Babesia gibsoni: identification of an immunodominant, interspersed repeat antigen
    Honglin Jia
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Exp Parasitol 118:146-9. 2008
    ..BgIRA plays a dominant role in the immune response in dogs infected with B. gibsoni. The specificity and sensitivity of the rBgIRA in an ELISA indicated that this antigen might be useful in a diagnostic test...
  19. pmc High-level expression and purification of a truncated merozoite antigen-2 of Babesia equi in Escherichia coli and its potential for immunodiagnosis
    Xiaohong Huang
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    J Clin Microbiol 41:1147-51. 2003
    ..The results indicate that G-rEMA-2t and rEMA-2t proteins should be suitable antigens for the development of an effective immunodiagnostic assay due to their high sensitivity, specificity, and great yield...
  20. doi request reprint Babesia gibsoni: identification, expression, localization, and serological characterization of a Babesia gibsoni 22-kDa protein
    Youn Kyoung Goo
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    Exp Parasitol 123:273-6. 2009
    ..Furthermore, it did not show a cross reaction with the closely related apicomplexan parasites, indicating that the rBgP22 could be used as a diagnostic antigen for a detection of the chronic carrier stages of B. gibsoni infection...
  21. pmc Inhibitory effect of antiserum to surface antigen P50 of Babesia gibsoni on growth of parasites in severe combined immunodeficiency mice given canine red blood cells
    Shinya Fukumoto
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    Infect Immun 72:1795-8. 2004
    ..The antiserum to the recombinant P50 protein significantly inhibited the parasite growth, indicating that P50 might be a useful vaccine candidate...
  22. ncbi request reprint Gene silencing of ribosomal protein P0 is lethal to the tick Haemaphysalis longicornis
    Haiyan Gong
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Vet Parasitol 151:268-78. 2008
    ..This suggests that ribosomal protein P0 is required for the blood ingestion and subsequent viability of H. longicornis. This is the first report of ribosomal protein P0 from ticks...
  23. doi request reprint TgGRA23, a novel Toxoplasma gondii dense granule protein associated with the parasitophorous vacuole membrane and intravacuolar network
    Tatsunori Masatani
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Parasitol Int 62:372-9. 2013
    ..Our results suggest that TgGRA23 is an as yet unknown member of the T. gondii dense granule proteins, and that it may be involved in remodeling or maintenance of the PV...
  24. doi request reprint Cloning, characterization and validation of inosine 5'-monophosphate dehydrogenase of Babesia gibsoni as molecular drug target
    Shinuo Cao
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido, Japan
    Parasitol Int 62:87-94. 2013
    ..95±0.21 and 2.88±0.49μM for B. gibsoni and B. bovis, respectively. Therefore, our results suggest that MPA may inhibit the replication of Babesia parasites by targeting IMPDH enzyme of the purine pathway...
  25. ncbi request reprint Apical membrane antigen 1 is a cross-reactive antigen between Neospora caninum and Toxoplasma gondii, and the anti-NcAMA1 antibody inhibits host cell invasion by both parasites
    Houshuang Zhang
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    Mol Biochem Parasitol 151:205-12. 2007
    ..caninum and T. gondii. These results indicated that AMA1 is a cross-reactive antigen between N. caninum and T. gondii and a potential common vaccine candidate to control two parasites...
  26. ncbi request reprint A 38-kDa protein from Babesia gibsoni and its antibody response in an experimentally infected dog
    Jinlin Zhou
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    Vet Parasitol 141:345-8. 2006
    ..gibsoni infection. These results allowed us to identify a new member of the 12D3 antigens and its characteristic immune response in canine B. gibsoni infection...
  27. ncbi request reprint Isolation and identification of an actin gene from Babesia gibsoni
    Jinlin Zhou
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    J Parasitol 92:208-10. 2006
    ..Immunofluorescence and confocal microscopic observation revealed that the protein is diffusely distributed throughout the B. gibsoni parasites...
  28. ncbi request reprint Development of two immunochromatographic tests for the serodiagnosis of bovine babesiosis
    Chulmin Kim
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Vet Parasitol 148:137-43. 2007
    ..8 and 92.5% relative to the results of standard ELISA and IFAT, respectively. Conventional and rapid diagnosic devices for bovine babesiosis may provide a valuable tool in clinical and field applications...
  29. doi request reprint Prime-boost vaccination with plasmid DNA followed by recombinant vaccinia virus expressing BgGARP induced a partial protective immunity to inhibit Babesia gibsoni proliferation in dogs
    Shinuo Cao
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido, 080 8555, Japan
    Acta Parasitol 58:619-23. 2013
    ..These results indicated that this type of DNA/rvv prime-boost immunization approach may have use against B. gibsoni infection in dogs. ..
  30. pmc Cloning of a novel Babesia equi gene encoding a 158-kilodalton protein useful for serological diagnosis
    Haruyuki Hirata
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Clin Diagn Lab Immunol 12:334-8. 2005
    ..equi-infected horse sera, indicating that the Be158 gene product is useful as a serologically diagnostic antigen for B. equi infection...
  31. ncbi request reprint Babesia gibsoni: Serodiagnosis of infection in dogs by an enzyme-linked immunosorbent assay with recombinant BgTRAP
    Youn Kyoung Goo
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Exp Parasitol 118:555-60. 2008
    ..gibsoni in dogs and that the combined ELISA/PCR approach could provide the most reliable diagnosis for clinical sites...
  32. ncbi request reprint Expression of C-terminal truncated and full-length Babesia bigemina rhoptry-associated protein 1 and their potential use in enzyme-linked immunosorbent assay
    Suthisak Boonchit
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    Vet Parasitol 137:28-35. 2006
    ..The high yield of soluble rRAP-1/CT and its diagnostic specificity demonstrate its potential in the diagnosis of B. bigemina infection. Its usefulness for epidemiological investigation is currently being evaluated...
  33. doi request reprint Immunogenicity and growth inhibitory efficacy of the prime-boost immunization regime with DNA followed by recombinant vaccinia virus carrying the P29 gene of Babesia gibsoni in dogs
    Shinya Fukumoto
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Exp Parasitol 123:296-301. 2009
    ..Moreover, the P29 immunized group showed a significantly low level of parasitemia. In conclusion, this study supports the efficacy of a prime-boost strategy for dogs against canine B. gibsoni infection...
  34. ncbi request reprint Babesia gibsoni: An apical membrane antigen-1 homologue and its antibody response in the infected dogs
    Jinlin Zhou
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Exp Parasitol 114:329-33. 2006
    ..This result shows that BgAMA-1 is a new member of the AMA-1 family and that its immune response is characteristic of canine B. gibsoni infection...
  35. pmc Dense-granule protein NcGRA7, a new marker for the serodiagnosis of Neospora caninum infection in aborting cows
    Penglong Huang
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Clin Vaccine Immunol 14:1640-3. 2007
    ..Our study suggested that NcGRA7 would be a new marker for the serodiagnosis of N. caninum infection resulting in abortion...
  36. pmc Improved enzyme-linked immunosorbent assay using C-terminal truncated recombinant antigens of Babesia bovis rhoptry-associated protein-1 for detection of specific antibodies
    Suthisak Boonchit
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    J Clin Microbiol 42:1601-4. 2004
    ..These results confirmed that the N-terminal 300-aa region caused cross-reactivity of the entire RAP-1 antigen, and the C-terminal truncated recombinant antigens were shown to be useful reagents for species-specific serodiagnosis...
  37. pmc Alpha-tocopherol transfer protein disruption confers resistance to malarial infection in mice
    Maria S Herbas
    Research Unit for Functional Genomics, National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Nishi 2 13, Inada, Obihiro, 080 8555 Japan
    Malar J 9:101. 2010
    ..However, mechanisms of this Plasmodium inhibition, in addition to means by which to exploit this finding as a therapeutic strategy, remain unclear...
  38. doi request reprint Molecular detection and identification of Babesia bovis and Babesia bigemina in cattle in northern Thailand
    Shinuo Cao
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido, 080 8555, Japan
    Parasitol Res 111:1259-66. 2012
    ..Overall, we have demonstrated a high prevalence and polymorphism of Babesia parasites in northern Thailand calling for the need to design effective control programs for bovine babesiosis...
  39. doi request reprint Protective effect of a prime-boost strategy with plasmid DNA followed by recombinant adenovirus expressing TgAMA1 as vaccines against Toxoplasma gondii infection in mice
    Longzheng Yu
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    Parasitol Int 61:481-6. 2012
    ..These results demonstrate that the heterologous DNA priming and recombinant adenovirus boost strategy may provide protective immunity against T. gondii infection...
  40. ncbi request reprint Serodiagnosis of Babesia gibsoni infection in dogs by an improved enzyme-linked immunosorbent assay with recombinant truncated P50
    Rodolfo A Verdida
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    J Vet Med Sci 66:1517-21. 2004
    ..8% (2/41) of tested samples were positive for dogs from Nanjing, China. These results suggest that the GST-P50t could be a reliable reagent for practical use in ELISA for the serodiagnosis of canine piroplasmosis caused by B. gibsoni...
  41. pmc Rapid immunochromatographic test using recombinant SAG2 for detection of antibodies against Toxoplasma gondii in cats
    Xiaohong Huang
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    J Clin Microbiol 42:351-3. 2004
    ..Evaluation of detection of the antibody in mice and cats suggests that this test is rapid, simple, accurate, relatively inexpensive, and suitable for use under field conditions...
  42. ncbi request reprint Expression of P23 of Cryptosporidium parvum in Toxoplasma gondii and evaluation of its protective effects
    Hiroaki Shirafuji
    National Research Center for Protozoan Disease, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokaido, Japan
    J Parasitol 91:476-9. 2005
    ..parvum. These findings indicate that the T. gondii vector may provide a new tool for the production of a recombinant vaccine against cryptosporidiosis in animals...
  43. doi request reprint A double antibody sandwich enzyme-linked immunosorbent assay for detection of secreted antigen 1 of Babesia microti using hamster model
    Yuzi Luo
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Exp Parasitol 130:178-82. 2012
    ..Taken together, these results indicated that BmSA1 could be a potential marker for surveillance of human babesiosis...
  44. pmc Immunochromatographic test for simultaneous serodiagnosis of Babesia caballi and B. equi infections in horses
    Xiaohong Huang
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Clin Vaccine Immunol 13:553-5. 2006
    ....
  45. pmc PCR detection and genetic diversity of bovine hemoprotozoan parasites in Vietnam
    Thillaiampalam Sivakumar
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    J Vet Med Sci 75:1455-62. 2013
    ..The findings suggest that hemoprotozoan parasites, some of which are genetically diverse, continue to be a threat to the livestock industry in this country. ..
  46. ncbi request reprint Epidemiological survey of Babesia bovis and Babesia bigemina infections of cattle in Philippines
    Longzheng Yu
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    J Vet Med Sci 75:995-8. 2013
    ..6%). Our data provide baseline information regarding the epidemiology of B. bovis and B. bigemina infections in cattle in Philippines, which can be utilized in developing proper strategies for disease control and management. ..
  47. doi request reprint Epidemiological survey of Babesia gibsoni infection in dogs in Japan by enzyme-linked immunosorbent assay using B. gibsoni thrombospondin-related adhesive protein antigen
    Kenji Konishi
    Department of Clinical Veterinary Science, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    Vet Parasitol 155:204-8. 2008
    ..gibsoni infection of non-fighting dogs in Japan and suggest that other B. gibsoni transmission routes, such as fighting and transplacental transmission, may be less important...
  48. doi request reprint Molecular prevalence of different genotypes of Theileria orientalis detected from cattle and water buffaloes in Thailand
    Khukhuu Altangerel
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    J Parasitol 97:1075-9. 2011
    ..orientalis in Thailand. In conclusion, T. orientalis parasites circulating in Thailand are more diverse in their genetic characters than previously anticipated...
  49. doi request reprint Molecular and immunological characterization of a novel 32-kDa secreted protein of Babesia microti
    Hideo Ooka
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    J Parasitol 98:1045-8. 2012
    ..microt i. However, high antibody titer was detected just before the challenge infection. Our data suggest that rBmP32 may be a specific diagnostic antigen but not a subunit vaccine...
  50. pmc Characterization of Toxoplasma gondii SAG2 expressed in insect cells by recombinant baculovirus and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay
    Xiaohong Huang
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    Clin Diagn Lab Immunol 9:1343-7. 2002
    ..The results suggest that S-rSAG2 would be a useful reagent for the detection of T. gondii infection in cats...
  51. ncbi request reprint Molecular characterization of Rhipicephalus (Boophilus) microplus Bm86 homologue from Haemaphysalis longicornis ticks
    Min Liao
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Vet Parasitol 146:148-57. 2007
    ..This is the first report of cloning and characterization of the Bm86 homologue in different genera and species of ixodid and argasid ticks since Bm86 was first reported in 1989...
  52. pmc Molecular cloning of a Babesia caballi gene encoding the 134-kilodalton protein and evaluation of its diagnostic potential in an enzyme-linked immunosorbent assay
    Yoh Tamaki
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    Clin Diagn Lab Immunol 11:211-5. 2004
    ..caballi-infected horse sera from Babesia equi-infected or noninfected control horse sera. These results suggest that the recombinant BC134 protein is a potential diagnostic antigen in the detection of B. caballi infection...
  53. ncbi request reprint A shared antigen among Babesia species: ribosomal phosphoprotein P0 as a universal babesial vaccine candidate
    M Alaa Terkawi
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido, 080 8555, Japan
    Parasitol Res 102:35-40. 2007
    ..Taken together, these data suggest that P0 is a conserved protective antigen among Babesia species and might be a potentially universal vaccine candidate for babesiosis...
  54. pmc Immunization with recombinant surface antigen P50 of Babesia gibsoni expressed in insect cells induced parasite growth inhibition in dogs
    Shinya Fukumoto
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan
    Clin Diagn Lab Immunol 12:557-9. 2005
    ..Dogs immunized with P50 showed partial protection manifested as a significantly low level of parasitemia. The results indicated that P50 is a primary vaccine candidate molecule against canine B. gibsoni infection...
  55. pmc Cloning, expression, and characterization of Babesia gibsoni dihydrofolate reductase-thymidylate synthase: inhibitory effect of antifolates on its catalytic activity and parasite proliferation
    Gabriel O Aboge
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido, Japan
    Antimicrob Agents Chemother 52:4072-80. 2008
    ..gibsoni DHFR could be a model for studying antifolate compounds as potential drug candidates. Therefore, the B. gibsoni DHFR-TS is a molecular antifolate drug target...
  56. doi request reprint Identification and characterization of an interspersed repeat antigen of Babesia microti (BmIRA)
    Shinuo Cao
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Exp Parasitol 133:346-52. 2013
    ..microti challenge, but no significant protection was observed. Our data suggest that the immunodominant antigen BmIRA could be a useful serodiagnostic antigen for screening of B. microti infection...
  57. ncbi request reprint Identification, cloning and characterization of BmP41, a common antigenic protein of Babesia microti
    Tatsunori Masatani
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    J Vet Med Sci 75:967-70. 2013
    ..microti Gray strain and mice infected with B. microti Munich strain. Taken together, BmP41 could be a promising universal serological marker for diagnosis of human babesiosis. ..
  58. doi request reprint Molecular characterization and antigenic properties of a novel Babesia gibsoni glutamic acid-rich protein (BgGARP)
    Ahmed Abdelmoniem Mousa
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan Department of Biochemistry and Chemistry of Nutrition, Faculty of Veterinary Medicine, Sadat City University, Sadat City, Menoufyia, Egypt
    Exp Parasitol 135:414-20. 2013
    ..Taken together, these results demonstrate that BgGARP is a suitable serodiagnostic antigen for detecting antibodies against B. gibsoni in dogs. ..
  59. ncbi request reprint Development of a rapid immunochromatographic test for simultaneous serodiagnosis of bovine babesioses caused by Babesia bovis and Babesia bigemina
    Chul Min Kim
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido, Japan
    Am J Trop Med Hyg 78:117-21. 2008
    ..bigemina were 96.7% (29 of 30) and 92.5% (74 of 80), respectively. These findings indicate that the BoiICT is useful for fast field diagnostic assessment of bovine babesioses without any laboratory equipment...
  60. ncbi request reprint Development of an immunochromatographic test with recombinant BgSA1 for the diagnosis of Babesia gibsoni infection in dogs
    Honglin Jia
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Parasitol Res 100:1381-4. 2007
    ..The concordance between the ELISA and the ICT was found to be 95.8%. These results suggested that the ICT using rBgSA1 is rapid, simple, accurate, and suitable for the diagnosis of B. gibsoni infection of dogs in the field...
  61. ncbi request reprint Characterization of the Babesia gibsoni P18 as a homologue of thrombospondin related adhesive protein
    Jinlin Zhou
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Mol Biochem Parasitol 148:190-8. 2006
    ..gibsoni and functionally important in merozoite invasion; this protein may be useful as a vaccine candidate against canine B. gibsoni infection...
  62. pmc Macrophages are critical for cross-protective immunity conferred by Babesia microti against Babesia rodhaini infection in mice
    Yan Li
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido, Japan
    Infect Immun 80:311-20. 2012
    ..We conclude that future development of vaccines against Babesia should include a strategy that enhances the appropriate activation of macrophages...
  63. ncbi request reprint Short report: prevalence of Neospora caninum and Toxoplasma gondii antibodies in northern Egypt
    Hany M Ibrahim
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido, Japan
    Am J Trop Med Hyg 80:263-7. 2009
    ..caninum positive (1.85%). The high prevalence of toxoplasmosis and neosporosis in cattle affects the development of the livestock industry and is also an important infective source for human infection in Egypt...
  64. pmc High-level expression of truncated surface antigen P50 of Babesia gibsoni in insect cells by baculovirus and evaluation of its immunogenicity and antigenicity
    Shinya Fukumoto
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    Clin Diagn Lab Immunol 10:596-601. 2003
    ..canis-infected dog serum or noninfected dog serum. Our results indicated that the rP50t may provide a useful potential immunogenic reagent for use in diagnosis and as a subunit vaccine to control B. gibsoni infection in dogs...
  65. doi request reprint Identification of the cross-reactive and species-specific antigens between Neospora caninum and Toxoplasma gondii tachyzoites by a proteomics approach
    Houshuang Zhang
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro, Hokkaido 080 8555, Japan
    Parasitol Res 109:899-911. 2011
    ..These observations have implications for the design of multicomponent common vaccines against both parasite infections...
  66. ncbi request reprint Serodiagnosis of ovine toxoplasmosis in Mongolia by an enzyme-linked immunosorbent assay with recombinant Toxoplasma gondii matrix antigen 1
    Buyannemekh Tumurjav
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Inada cho, Obihiro 080 8555, Japan
    Jpn J Vet Res 58:111-9. 2010
    ..Of 29 LAT-positive samples, 27 (93.10%) were positive by the ELISA. These results suggest that rTgMAG1 could be used as a reliable antigen for the detection of T. gondii infection in sheep...
  67. doi request reprint Babesia microti: molecular and antigenic characterizations of a novel 94-kDa protein (BmP94)
    Hideo Ooka
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    Exp Parasitol 127:287-93. 2011
    ..microti challenge, but no significant protection was observed. Our data suggest that the immunodominant antigen BmP94 could be a promising candidate for diagnostic use for human babesiosis...
  68. doi request reprint Development of a rapid immunochromatographic test using a recombinant thrombospondin-related adhesive protein of Babesia gibsoni
    Youn Kyoung Goo
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    Vet Parasitol 190:595-8. 2012
    ..The developed ICT showed high sensitivity, specificity, and kappa value with a reference test (100%, 93.78%, and 0.8976, respectively), indicating that the ICT could be a new practical diagnostic test for B. gibsoni infection...
  69. doi request reprint Expression of truncated Babesia gibsoni thrombospondin-related adhesive proteins in Escherichia coli and evaluation of their diagnostic potential by enzyme-linked immunosorbent assay
    S Narantsatsral
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    Exp Parasitol 129:196-202. 2011
    ..8003) than rBgTRAPn (0.7083). With a sufficient level of expression as well as higher specificity and reliable sensitivity, rBgTRAPc appears to be a potential candidate antigen for the serodiagnosis of B. gibsoni infection in dogs...
  70. ncbi request reprint Viability and infectivity of Cryptosporidium parvum oocysts detected in river water in Hokkaido, Japan
    Yoshinori Tsushima
    National Research Center for Protozoan Diseases, Obihiro University, Hokkaido, Japan
    J Vet Med Sci 65:585-9. 2003
    ..parvum genotype 2. Moreover, our data on the continued isolation, detection and identification of the C. parvum isolates is consistent with the available epidemiological data for the Tokachi area...
  71. pmc Roles of the Maltese cross form in the development of parasitemia and protection against Babesia microti infection in mice
    Naoaki Yokoyama
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080 8555, Japan
    Infect Immun 71:411-7. 2003
    ..microti infection. These findings indicate that the Maltese cross form plays important roles in both the development of parasitemia and the protective response against the infection...
  72. ncbi request reprint Identification of a novel gene encoding a secreted antigen 1 of Babesia gibsoni and evaluation of its use in serodiagnosis
    Honglin Jia
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan
    Am J Trop Med Hyg 75:843-50. 2006
    ..gibsoni. These results indicated that BgSA1 could be a useful target for the development of a diagnostic test for the detection of specific antibodies and circulating antigens...
  73. doi request reprint Artesunate, a potential drug for treatment of Babesia infection
    Youn Kyoung Goo
    National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido, Japan
    Parasitol Int 59:481-6. 2010
    ..microti at doses equal to and greater than 10mg/kg of body weight on days 8-10 post-infection. Taken together, these results suggest that artesunate could be a potential drug against Babesia infection...