Mitinori Saitou

Summary

Affiliation: RIKEN Brain Science Institute
Country: Japan

Publications

  1. ncbi request reprint A molecular programme for the specification of germ cell fate in mice
    Mitinori Saitou
    Wellcome Trust Cancer Research UK Institute of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QR, UK
    Nature 418:293-300. 2002
  2. ncbi request reprint Gene expression dynamics during germline specification in mice identified by quantitative single-cell gene expression profiling
    Yukihiro Yabuta
    Laboratory for Mammalian Germ Cell Biology, Center for Developmental Biology, RIKEN Kobe Institute, Kobe 650 0047, Japan
    Biol Reprod 75:705-16. 2006
  3. pmc An improved single-cell cDNA amplification method for efficient high-density oligonucleotide microarray analysis
    Kazuki Kurimoto
    Laboratory for Mammalian Germ Cell Biology, Center for Developmental Biology, RIKEN Kobe Institute, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe, Hyogo 650 0047, Japan
    Nucleic Acids Res 34:e42. 2006
  4. ncbi request reprint Single-cell cDNA high-density oligonucleotide microarray analysis: detection of individual cell types and properties in complex biological processes
    Mitinori Saitou
    Laboratory for Mammalian Germ Cell Biology, Centre for Developmental Biology, RIKEN Kobe Institute, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe 650 0047, Japan
    Reprod Biomed Online 16:26-40. 2008
  5. ncbi request reprint [Origin and properties of the germ cell lineage in mice]
    Mitinori Saitou
    Tanpakushitsu Kakusan Koso 51:1058-71. 2006
  6. ncbi request reprint Specification of the germ cell lineage in mice
    Mitinori Saitou
    Laboratory for Mammalian Germ Cell Biology, Center for Developmental Biology, RIKEN Kobe Institute, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe 650 0047, Japan
    Front Biosci (Landmark Ed) 14:1068-87. 2009
  7. ncbi request reprint Functional reconstruction of NANOS3 expression in the germ cell lineage by a novel transgenic reporter reveals distinct subcellular localizations of NANOS3
    Masashi Yamaji
    Laboratory for Mammalian Germ Cell Biology, RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe 650 0047, Japan
    Reproduction 139:381-93. 2010
  8. ncbi request reprint A signaling principle for the specification of the germ cell lineage in mice
    Yasuhide Ohinata
    Center for Developmental Biology, RIKEN Kobe Institute, Minatojima Minamimachi, Chuo Ku, Japan
    Cell 137:571-84. 2009
  9. ncbi request reprint Cellular dynamics associated with the genome-wide epigenetic reprogramming in migrating primordial germ cells in mice
    Yoshiyuki Seki
    Laboratory for Mammalian Germ Cell Biology, Center for Developmental Biology, RIKEN Kobe Institute, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe, 650 0047, Japan
    Development 134:2627-38. 2007
  10. ncbi request reprint Critical function of Prdm14 for the establishment of the germ cell lineage in mice
    Masashi Yamaji
    Laboratory for Mammalian Germ Cell Biology, Center for Developmental Biology, RIKEN Kobe Institute, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe 650 0047, Japan
    Nat Genet 40:1016-22. 2008

Collaborators

Detail Information

Publications30

  1. ncbi request reprint A molecular programme for the specification of germ cell fate in mice
    Mitinori Saitou
    Wellcome Trust Cancer Research UK Institute of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QR, UK
    Nature 418:293-300. 2002
    ..The stella positive nascent germ cells exhibit repression of homeobox genes, which may explain their escape from a somatic cell fate and the retention of pluripotency...
  2. ncbi request reprint Gene expression dynamics during germline specification in mice identified by quantitative single-cell gene expression profiling
    Yukihiro Yabuta
    Laboratory for Mammalian Germ Cell Biology, Center for Developmental Biology, RIKEN Kobe Institute, Kobe 650 0047, Japan
    Biol Reprod 75:705-16. 2006
    ....
  3. pmc An improved single-cell cDNA amplification method for efficient high-density oligonucleotide microarray analysis
    Kazuki Kurimoto
    Laboratory for Mammalian Germ Cell Biology, Center for Developmental Biology, RIKEN Kobe Institute, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe, Hyogo 650 0047, Japan
    Nucleic Acids Res 34:e42. 2006
    ..This study provides a strategy to analyze biophysical events in medicine as well as in neural, stem cell and developmental biology, where small numbers of distinctive or diseased cells play critical roles...
  4. ncbi request reprint Single-cell cDNA high-density oligonucleotide microarray analysis: detection of individual cell types and properties in complex biological processes
    Mitinori Saitou
    Laboratory for Mammalian Germ Cell Biology, Centre for Developmental Biology, RIKEN Kobe Institute, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe 650 0047, Japan
    Reprod Biomed Online 16:26-40. 2008
    ....
  5. ncbi request reprint [Origin and properties of the germ cell lineage in mice]
    Mitinori Saitou
    Tanpakushitsu Kakusan Koso 51:1058-71. 2006
  6. ncbi request reprint Specification of the germ cell lineage in mice
    Mitinori Saitou
    Laboratory for Mammalian Germ Cell Biology, Center for Developmental Biology, RIKEN Kobe Institute, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe 650 0047, Japan
    Front Biosci (Landmark Ed) 14:1068-87. 2009
    ..The accumulated knowledge will be critical for the reconstitution of this key lineage in vitro, which may provide a useful foundation for reproductive and regenerative medicine...
  7. ncbi request reprint Functional reconstruction of NANOS3 expression in the germ cell lineage by a novel transgenic reporter reveals distinct subcellular localizations of NANOS3
    Masashi Yamaji
    Laboratory for Mammalian Germ Cell Biology, RIKEN Center for Developmental Biology, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe 650 0047, Japan
    Reproduction 139:381-93. 2010
    ..These findings unveil the presence of distinct posttranscriptional regulations in PGCs soon after their specification, for which RNA-binding proteins such as NANOS3 and TIAL1 would play critical functions...
  8. ncbi request reprint A signaling principle for the specification of the germ cell lineage in mice
    Yasuhide Ohinata
    Center for Developmental Biology, RIKEN Kobe Institute, Minatojima Minamimachi, Chuo Ku, Japan
    Cell 137:571-84. 2009
    ..By identifying a signaling principle in germ cell specification, our study establishes a robust strategy for reconstituting the mammalian germ cell lineage in vitro...
  9. ncbi request reprint Cellular dynamics associated with the genome-wide epigenetic reprogramming in migrating primordial germ cells in mice
    Yoshiyuki Seki
    Laboratory for Mammalian Germ Cell Biology, Center for Developmental Biology, RIKEN Kobe Institute, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe, 650 0047, Japan
    Development 134:2627-38. 2007
    ..We suggest the possibility that active repression of an essential enzyme and subsequent unique cellular dynamics ensures successful implementation of genome-wide epigenetic reprogramming in migrating PGCs...
  10. ncbi request reprint Critical function of Prdm14 for the establishment of the germ cell lineage in mice
    Masashi Yamaji
    Laboratory for Mammalian Germ Cell Biology, Center for Developmental Biology, RIKEN Kobe Institute, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe 650 0047, Japan
    Nat Genet 40:1016-22. 2008
    ..Our combined evidence demonstrates that Prdm14 defines a previously unknown genetic pathway, initiating independently from Prdm1, for ensuring the launching of the mammalian germ cell lineage...
  11. ncbi request reprint Specification of the germ cell lineage in mice: a process orchestrated by the PR-domain proteins, Blimp1 and Prdm14
    Kazuki Kurimoto
    Laboratory for Mammalian Germ Cell Biology, RIKEN Center for Developmental Biology, Chuo Ku, Kobe, Japan
    Cell Cycle 7:3514-8. 2008
    ..The launch of the germ cell lineage in mice, therefore, is orchestrated by two independently acquired, PR domain-containing transcriptional regulators, Blimp1 and Prdm14...
  12. pmc Complex genome-wide transcription dynamics orchestrated by Blimp1 for the specification of the germ cell lineage in mice
    Kazuki Kurimoto
    Laboratory for Mammalian Germ Cell Biology, Center for Developmental Biology, RIKEN Kobe Institute, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe 650 0047, Japan
    Genes Dev 22:1617-35. 2008
    ..Thus, Blimp1 is not a single initiator but a dominant coordinator of the transcriptional program for the establishment of the germ cell fate in mice...
  13. ncbi request reprint Blimp1 and the emergence of the germ line during development in the mouse
    Mitinori Saitou
    Laboratory for Mammalian Germ Cell Biology, Center for Developmental Biology, RIKEN Kobe Institute, Kobe, Japan
    Cell Cycle 4:1736-40. 2005
    ..Disruption of Blimp1 function resulted in aberrant PGC-like cells with a deregulated intrinsic gene expression program at a very early stage, which demonstrates that Blimp1 is a critical determinant of the germ line in mice...
  14. ncbi request reprint A comprehensive, non-invasive visualization of primordial germ cell development in mice by the Prdm1-mVenus and Dppa3-ECFP double transgenic reporter
    Yasuhide Ohinata
    Laboratory for Mammalian Germ Cell Biology, RIKEN Kobe Institute, Center for Developmental Biology, Chuo Ku, Kobe 650 0047, Japan
    Reproduction 136:503-14. 2008
    ....
  15. ncbi request reprint Male germline and embryonic stem cell lines from NOD mice: efficient derivation of GS cells from a nonpermissive strain for ES cell derivation
    Hiroshi Ohta
    Laboratories for Genomic Reprogramming, Center for Developmental Biology, RIKEN, Kobe, Japan
    Biol Reprod 81:1147-53. 2009
    ..Our results also suggest that the establishment of GS cells is an effective procedure in nonpermissive mouse strains or other species for ES cell derivation...
  16. ncbi request reprint Global single-cell cDNA amplification to provide a template for representative high-density oligonucleotide microarray analysis
    Kazuki Kurimoto
    Laboratory for Mammalian Germ Cell Biology, Center for Developmental Biology, RIKEN Kobe Institute, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe 650 0047, Japan
    Nat Protoc 2:739-52. 2007
    ..This protocol yields cDNA templates sufficient for more than 80 microarray hybridizations from a single cell, and can be completed in 5-6 days...
  17. ncbi request reprint Single-cell gene profiling defines differential progenitor subclasses in mammalian neurogenesis
    Ayano Kawaguchi
    Laboratory for Cell Asymmetry, Center for Developmental Biology, RIKEN Kobe Institute, 2 2 3 Minatojima minamimachi, Chuo Ku, Kobe, Hyogo 650 0047, Japan
    Development 135:3113-24. 2008
    ..Thus, a Notch-dependent feedback loop is likely to be in operation to maintain both progenitor populations...
  18. ncbi request reprint Blimp1 is a critical determinant of the germ cell lineage in mice
    Yasuhide Ohinata
    Laboratory for Mammalian Germ Cell Biology, Center for Developmental Biology, RIKEN Kobe Institute, 2 2 3 Minatojima minamimachi, Kobe, Hyogo 650 0047, Japan
    Nature 436:207-13. 2005
    ..Furthermore, our genetic lineage-tracing experiments indicate that the Blimp1-positive cells originating from the proximal posterior epiblast cells are indeed the lineage-restricted primordial germ cell precursors...
  19. ncbi request reprint Extensive and orderly reprogramming of genome-wide chromatin modifications associated with specification and early development of germ cells in mice
    Yoshiyuki Seki
    Department of Molecular Embryology, Research Institute, Osaka Medical Center for Maternal and Child Health, Izumi, Osaka 594 1101, Japan
    Dev Biol 278:440-58. 2005
    ....
  20. ncbi request reprint The maternal nucleolus is essential for early embryonic development in mammals
    Sugako Ogushi
    Graduate School of Science and Technology, Kobe University, Kobe 657 8501, Japan
    Science 319:613-6. 2008
    ..In addition, the nucleolus in the embryo produced by somatic cell nuclear transfer originates from the oocyte, demonstrating that the maternal nucleolus supports successful embryonic development...
  21. ncbi request reprint [Epigenetic reprogramming in germ cells]
    Yoshiyuki Seki
    Tanpakushitsu Kakusan Koso 51:2052-9. 2006
  22. ncbi request reprint A global single-cell cDNA amplification method for quantitative microarray analysis
    Kazuki Kurimoto
    Laboratory for Mammalian Germ Cell Biology, RIKEN Center for Developmental Biology, Kobe, Japan
    Methods Mol Biol 687:91-111. 2011
    ..The promoter-tagged cDNA is purified with gel electrophoresis and amplified with one final cycle of PCR...
  23. ncbi request reprint The nucleolus in the mouse oocyte is required for the early step of both female and male pronucleus organization
    Sugako Ogushi
    RIKEN Center for Developmental Biology, Kobe, Japan
    J Reprod Dev 56:495-501. 2010
    ..Thus, the critical time point when the nucleolus is required for progression of early embryonic development appears to be at the point of the early step of pronucleus organization...
  24. ncbi request reprint Survival and death of epiblast cells during embryonic stem cell derivation revealed by long-term live-cell imaging with an Oct4 reporter system
    Kazuo Yamagata
    Laboratory for Genomic Reprogramming, Center for Developmental Biology, RIKEN Kobe, Minatojima minamimachi 2 2 3, Chuo Ku, Kobe City, Hyogo 651 0047, Japan
    Dev Biol 346:90-101. 2010
    ..Thus, our imaging technique can advance the understanding of the time-dependent cellular changes during ESC derivation...
  25. ncbi request reprint [A molecular programme for the germ line specification in mice]
    Mitinori Saitou
    Wellcome Trust Cancer Research UK Institute, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QR, UK
    Seikagaku 75:42-6. 2003
  26. pmc Specification of germ cell fate in mice
    Mitinori Saitou
    Wellcome Trust Cancer Research UK Institute, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, UK
    Philos Trans R Soc Lond B Biol Sci 358:1363-70. 2003
    ..While these molecules are also detected in mature oocytes as maternally inherited factors, their early role is to regulate development and maintain pluripotency, and they do not serve the role of classical germline determinants...
  27. ncbi request reprint Stella is a maternal effect gene required for normal early development in mice
    Bernhard Payer
    Wellcome Trust Cancer Research UK Institute of Cancer and Developmental Biology, University of Cambridge, Tennis Court Road, Cambridge CB2 1QR, United Kingdom
    Curr Biol 13:2110-7. 2003
    ..These findings suggest a similar role for STELLA during early human development as in mice and a potential involvement in germ cell tumors...
  28. ncbi request reprint Analysis of Esg1 expression in pluripotent cells and the germline reveals similarities with Oct4 and Sox2 and differences between human pluripotent cell lines
    Patrick Western
    ARC Centre of Excellence in Biotechnology and Development and Murdoch Children s Research Institute, Royal Children s Hospital, Melbourne, Victoria, Australia
    Stem Cells 23:1436-42. 2005
    ..These data suggest that together with Oct4 and Sox2, Esg1 plays a conserved role in the pluripotent pathway of mouse and human stem and germ cells...
  29. ncbi request reprint Generation of stella-GFP transgenic mice: a novel tool to study germ cell development
    Bernhard Payer
    Wellcome Trust Cancer Research UK Gurdon Institute of Cancer and Developmental Biology, University of Cambridge, Cambridge, United Kingdom
    Genesis 44:75-83. 2006
    ..Additionally, we demonstrate the usefulness of stella-GFP during the derivation of PGCs from ES cells...
  30. pmc Blimp1 defines a progenitor population that governs cellular input to the sebaceous gland
    Valerie Horsley
    Laboratory of Mammalian Cell Biology and Development, Howard Hughes Medical Institute, The Rockefeller University, New York, NY 10021, USA
    Cell 126:597-609. 2006
    ....