Tsuyoshi Yamada

Summary

Affiliation: Teikyo University
Country: Japan

Publications

  1. ncbi request reprint Genetic transformation of the dermatophyte, Trichophyton mentagrophytes, based on the use of G418 resistance as a dominant selectable marker
    Tsuyoshi Yamada
    Teikyo University Institute of Medical Mycology, Teikyo University, Hachioji, Tokyo 192 0395, Japan
    J Dermatol Sci 49:53-61. 2008
  2. doi request reprint Enhanced gene replacements in Ku80 disruption mutants of the dermatophyte, Trichophyton mentagrophytes
    Tsuyoshi Yamada
    Teikyo University Institute of Medical Mycology, Teikyo University, Tokyo, Japan
    FEMS Microbiol Lett 298:208-17. 2009
  3. doi request reprint Agrobacterium tumefaciens-mediated transformation of the dermatophyte, Trichophyton mentagrophytes: an efficient tool for gene transfer
    Tsuyoshi Yamada
    Teikyo University Institute of Medical Mycology, Teikyo University, Hachioji, Tokyo, Japan
    Med Mycol 47:485-94. 2009
  4. doi request reprint Nourseothricin acetyltransferase: a new dominant selectable marker for the dermatophyte Trichophyton mentagrophytes
    Mohamed Mahdi Alshahni
    Teikyo University Institute of Medical Mycology, Hachioji, Tokyo, Japan
    Med Mycol 48:665-8. 2010
  5. ncbi request reprint Reproducible genetic transformation system for two dermatophytes, Microsporum canis and Trichophyton mentagrophytes
    Tsuyoshi Yamada
    Teikyo University Institute of Medical Mycology, Hachioji, Tokyo, Japan
    Med Mycol 43:533-44. 2005
  6. doi request reprint Suppression of anti-Candida activity of macrophages by a quorum-sensing molecule, farnesol, through induction of oxidative stress
    Shigeru Abe
    Teikyo University Institute of Medical Mycology, 359 Otsuka, Hachioji, Tokyo 192 0395, Japan
    Microbiol Immunol 53:323-30. 2009
  7. ncbi request reprint Isolation, characterization, and disruption of dnr1, the areA/nit-2-like nitrogen regulatory gene of the zoophilic dermatophyte, Microsporum canis
    Tsuyoshi Yamada
    Teikyo University Institute of Medical Mycology, Hachioji, Tokyo, Japan
    Med Mycol 44:243-52. 2006
  8. doi request reprint Insights into a nonhomologous integration pathway in the dermatophyte Trichophyton mentagrophytes: efficient targeted gene disruption by use of mutants lacking ligase IV
    Mohamed Mahdi Alshahni
    Teikyo University Institute of Medical Mycology, Hachioji, Tokyo, Japan
    Microbiol Immunol 55:34-43. 2011
  9. doi request reprint Genetic and Phenotypic analyses of Calcineurin A subunit in Arthroderma vanbreuseghemii
    Mohamed Mahdi Alshahni
    Laboratory of Space and Environmental Medicine, Graduate School of Medicine, Teikyo University, 2 11 1, Kaga, Itabashi, Tokyo 173 8605, Japan
    Med Mycol 54:207-18. 2016
  10. doi request reprint Rapid real-time diagnostic PCR for Trichophyton rubrum and Trichophyton mentagrophytes in patients with tinea unguium and tinea pedis using specific fluorescent probes
    Yoshiharu Miyajima
    Teikyo University Institute of Medical Mycology and Genome Research Center, Tokyo, Japan
    J Dermatol Sci 69:229-35. 2013

Collaborators

Detail Information

Publications18

  1. ncbi request reprint Genetic transformation of the dermatophyte, Trichophyton mentagrophytes, based on the use of G418 resistance as a dominant selectable marker
    Tsuyoshi Yamada
    Teikyo University Institute of Medical Mycology, Teikyo University, Hachioji, Tokyo 192 0395, Japan
    J Dermatol Sci 49:53-61. 2008
    ..In dermatophytes, only the hygromycin B phosphotransferase gene (hph) is available as a selectable marker...
  2. doi request reprint Enhanced gene replacements in Ku80 disruption mutants of the dermatophyte, Trichophyton mentagrophytes
    Tsuyoshi Yamada
    Teikyo University Institute of Medical Mycology, Teikyo University, Tokyo, Japan
    FEMS Microbiol Lett 298:208-17. 2009
    ..Ku80(-) mutant strains will be useful for large-scale reverse genetics studies of dermatophytes, including T. mentagrophytes, providing valuable information on the basic mechanisms of host invasion...
  3. doi request reprint Agrobacterium tumefaciens-mediated transformation of the dermatophyte, Trichophyton mentagrophytes: an efficient tool for gene transfer
    Tsuyoshi Yamada
    Teikyo University Institute of Medical Mycology, Teikyo University, Hachioji, Tokyo, Japan
    Med Mycol 47:485-94. 2009
    ....
  4. doi request reprint Nourseothricin acetyltransferase: a new dominant selectable marker for the dermatophyte Trichophyton mentagrophytes
    Mohamed Mahdi Alshahni
    Teikyo University Institute of Medical Mycology, Hachioji, Tokyo, Japan
    Med Mycol 48:665-8. 2010
    ..Molecular analysis showed integration of the NAT cassette into the genomic DNA of T. mentagrophytes. This study presents the nourseothricin resistance gene nat1 as a useful selectable marker for selection of T. mentagrophytes...
  5. ncbi request reprint Reproducible genetic transformation system for two dermatophytes, Microsporum canis and Trichophyton mentagrophytes
    Tsuyoshi Yamada
    Teikyo University Institute of Medical Mycology, Hachioji, Tokyo, Japan
    Med Mycol 43:533-44. 2005
    ..This reproducible transformation system provides a method for the genetic manipulation of these pathogens, which will facilitate detailed molecular analysis of dermatophytosis...
  6. doi request reprint Suppression of anti-Candida activity of macrophages by a quorum-sensing molecule, farnesol, through induction of oxidative stress
    Shigeru Abe
    Teikyo University Institute of Medical Mycology, 359 Otsuka, Hachioji, Tokyo 192 0395, Japan
    Microbiol Immunol 53:323-30. 2009
    ..These effects of farnesol were blocked by probucol or trolox. These results indicate that farnesol lowered viability of the murine macrophages and suppressed their anti-Candida activity, perhaps through induction of ROS...
  7. ncbi request reprint Isolation, characterization, and disruption of dnr1, the areA/nit-2-like nitrogen regulatory gene of the zoophilic dermatophyte, Microsporum canis
    Tsuyoshi Yamada
    Teikyo University Institute of Medical Mycology, Hachioji, Tokyo, Japan
    Med Mycol 44:243-52. 2006
    ..This is the first report describing successful production of targeted gene-disrupted mutants by homologous recombination and their phenotypic analysis in dermatophytes...
  8. doi request reprint Insights into a nonhomologous integration pathway in the dermatophyte Trichophyton mentagrophytes: efficient targeted gene disruption by use of mutants lacking ligase IV
    Mohamed Mahdi Alshahni
    Teikyo University Institute of Medical Mycology, Hachioji, Tokyo, Japan
    Microbiol Immunol 55:34-43. 2011
    ..These results suggest that studies in strains lacking TmLIG4 would help to improve our understanding of dermatophytosis by facilitating the genetic manipulation of dermatophytes...
  9. doi request reprint Genetic and Phenotypic analyses of Calcineurin A subunit in Arthroderma vanbreuseghemii
    Mohamed Mahdi Alshahni
    Laboratory of Space and Environmental Medicine, Graduate School of Medicine, Teikyo University, 2 11 1, Kaga, Itabashi, Tokyo 173 8605, Japan
    Med Mycol 54:207-18. 2016
    ..In conclusion, calcineurin A is a multifunctional gene that is involved in the regulation of several biological processes and therefore is worth being considered as a drug target for treatment of dermatophytoses. ..
  10. doi request reprint Rapid real-time diagnostic PCR for Trichophyton rubrum and Trichophyton mentagrophytes in patients with tinea unguium and tinea pedis using specific fluorescent probes
    Yoshiharu Miyajima
    Teikyo University Institute of Medical Mycology and Genome Research Center, Tokyo, Japan
    J Dermatol Sci 69:229-35. 2013
    ..For suitable diagnosis and treatment, rapid and accurate identification of etiologic agents in clinical samples using reliable molecular based method is required...
  11. doi request reprint Protective effects of farnesol against oral candidiasis in mice
    Tatsuya Hisajima
    Teikyo University Institute of Medical Mycology, Tokyo, Japan
    Microbiol Immunol 52:327-33. 2008
    ..albicans cells not only on tongues but also in feces, kidneys and livers. These results suggest that farnesol has very characteristic roles in protection against mucosal candidiasis...
  12. ncbi request reprint Direct colony PCR of several medically important fungi using Ampdirect plus
    Mohamed M Alshahni
    Teikyo University Institute of Medical Mycology, Tokyo 192 0395, Japan
    Jpn J Infect Dis 62:164-7. 2009
    ..Ampdirect DCPCR was also applicable to DNA amplification for spore and hyphal cells. This approach reduces DNA template preparation time before PCR from fungal colonies, and also reduces the cost of PCR...
  13. pmc Mechanism of Action of ME1111, a Novel Antifungal Agent for Topical Treatment of Onychomycosis
    Sho Takahata
    Meiji Seika Pharma Co, Ltd, Tokyo, Japan
    Antimicrob Agents Chemother 60:873-80. 2016
    ..Thus, ME1111 is a novel inhibitor of the succinate dehydrogenase of Trichophyton species, and its mechanism of action indicates its selective profile. ..
  14. ncbi request reprint Development of efficient tools for genetic manipulation of dermatophytes
    Tsuyoshi Yamada
    Teikyo University Institute of Medical Mycology, Hachioji, Tokyo, Japan
    Nihon Ishinkin Gakkai Zasshi 51:87-92. 2010
    ..To overcome these problems, our group has developed useful genetic manipulation systems for dermatophytes using the clinically important dermatophyte, T. mentagrophytes...
  15. ncbi request reprint Isolation of a promoter region of a secreted metalloprotease gene from Microsporum canis
    Tsuyoshi Yamada
    Teikyo University Institute of Medical Mycology, Tokyo 192 0395, Japan
    Jpn J Infect Dis 57:25-8. 2004
    ..Further analyses of the regulatory sequence may be useful for understanding the molecular basis of the coordinated expression of the various genes involved in dermatophytosis...
  16. ncbi request reprint [Molecular approach to pathology of and immunity against dermatophytes]
    Tsuyoshi Yamada
    Teikyo University Institute of Medical Mycology, Tokyo, Japan
    Nihon Ishinkin Gakkai Zasshi 49:293-7. 2008
    ..We have developed an efficient dermatophyte genetic manipulation system using T. mentagrophytes. Here, we present our current research findings, mainly with regard to the system for exogenous gene transfer into T. mentagrophytes cells...
  17. ncbi request reprint Genetic Manipulations in Dermatophytes
    Mohamed Mahdi Alshahni
    Laboratory of Space and Environmental Medicine, Graduate School of Medicine, Teikyo University, 2 11 1, Kaga, Itabashi, Tokyo, 173 8605, Japan
    Mycopathologia . 2016
    ..In conclusion, the establishment of basic molecular tools and the availability of genomic data will open a new era that might change our understanding on the biology and pathogenicity of this fungal group...
  18. ncbi request reprint Metalloprotease gene of Arthroderma gypseum
    Rui Kano
    Department of Pathobiology, Nihon University School of Veterinary Medicine, Kanagawa 252 8510, Japan
    Jpn J Infect Dis 58:214-7. 2005
    ..gypseum. Reverse transcriptase-PCR analysis suggested that keratin might stimulate the expression of MEP mRNA in A. gypseum...