Kunio Ohyama

Summary

Affiliation: Tokyo University of Pharmacy and Life Science
Country: Japan

Publications

  1. ncbi request reprint Presence of Vicia graminea lectin- and Vicia unijuga lectin-binding (Vgu) glycoproteins in human fetal membranes and some of their biochemical properties
    K Ohyama
    Department of Biochemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan
    Int J Biochem Cell Biol 30:609-22. 1998
  2. ncbi request reprint Human gastric signet ring carcinoma (KATO-III) cell apoptosis induced by Vitex agnus-castus fruit extract through intracellular oxidative stress
    Kunio Ohyama
    Department of Clinical Molecular Genetics, Faculty of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432 1 Horinouchi, Hachioji, Tokyo 192 0355, Japan
    Int J Biochem Cell Biol 37:1496-510. 2005
  3. ncbi request reprint Predominant contribution of IFN-beta expression to apoptosis induction in human uterine cervical fibroblast cells by influenza-virus infection
    Kunio Ohyama
    Department of Clinical Molecular Genetics, Faculty of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo, Japan
    Biol Pharm Bull 27:1750-7. 2004
  4. ncbi request reprint Apoptosis induced by influenza virus-hemagglutinin stimulation may be related to fluctuation of cellular oxidative condition
    Kunio Ohyama
    Department of Biochemistry, Faculty of Pharmacy, Tokyo University of Pharmacy and Life Science, Horinouchi, Hachioji, Tokyo, Japan
    Biol Pharm Bull 26:141-7. 2003
  5. ncbi request reprint Cytotoxicity and apoptotic inducibility of Vitex agnus-castus fruit extract in cultured human normal and cancer cells and effect on growth
    Kunio Ohyama
    Department of Biochemistry, Faculty of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432 1 Horinouchi, Hachioji, Tokyo 192 0355, Japan
    Biol Pharm Bull 26:10-8. 2003
  6. ncbi request reprint Progressive apoptosis in chorion laeve trophoblast cells of human fetal membrane tissues during in vitro incubation is suppressed by antioxidative reagents
    K Ohyama
    Department of Biochemistry, Faculty of Pharmacy, Tokyo University of Pharmacy and Life Science, Tokyo, Japan
    Eur J Biochem 268:6182-9. 2001
  7. ncbi request reprint Biosynthesis of Vicia graminea lectin- and Vicia unijuga lectin-binding glycoproteins in human tumor and nontumor cells and an estimation of its epitope structure
    K Ohyama
    Department of Biochemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Japan
    Cancer Detect Prev 24:61-71. 2000
  8. ncbi request reprint Improvement of separation method of fragmented DNA from an apoptotic cell DNA sample for the quantitation using agarose gel electrophoresis
    K Ohyama
    Department of Biochemistry, Tokyo University of Pharmacy and Life Science, Hachioji, Japan
    Biol Pharm Bull 24:342-6. 2001
  9. doi request reprint Cytotoxic effects of flavonoids against a human colon cancer derived cell line, COLO 201: a potential natural anti-cancer substance
    Masahiko Imai
    Department of Clinical Molecular Genetics, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432 1 Horinouchi, Hachioji, Tokyo 192 0392, Japan
    Cancer Lett 276:74-80. 2009
  10. ncbi request reprint Effects of mitogen-activated protein kinase inhibitors on tumor necrosis factor-alpha gene expression and apoptosis induction in cultured human fetal membrane chorion cells infected with influenza virus
    Noboru Uchide
    Department of Clinical Molecular Genetics, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Tokyo, Japan
    Intervirology 50:99-107. 2007

Collaborators

Detail Information

Publications28

  1. ncbi request reprint Presence of Vicia graminea lectin- and Vicia unijuga lectin-binding (Vgu) glycoproteins in human fetal membranes and some of their biochemical properties
    K Ohyama
    Department of Biochemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Japan
    Int J Biochem Cell Biol 30:609-22. 1998
    ....
  2. ncbi request reprint Human gastric signet ring carcinoma (KATO-III) cell apoptosis induced by Vitex agnus-castus fruit extract through intracellular oxidative stress
    Kunio Ohyama
    Department of Clinical Molecular Genetics, Faculty of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432 1 Horinouchi, Hachioji, Tokyo 192 0355, Japan
    Int J Biochem Cell Biol 37:1496-510. 2005
    ..Our results demonstrate that intracellular oxidative stress and mitochondrial membrane damage is responsible for Vitex-induced apoptosis, which may be mediated by a diminution of reduced type glutathione within the cell...
  3. ncbi request reprint Predominant contribution of IFN-beta expression to apoptosis induction in human uterine cervical fibroblast cells by influenza-virus infection
    Kunio Ohyama
    Department of Clinical Molecular Genetics, Faculty of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Tokyo, Japan
    Biol Pharm Bull 27:1750-7. 2004
    ....
  4. ncbi request reprint Apoptosis induced by influenza virus-hemagglutinin stimulation may be related to fluctuation of cellular oxidative condition
    Kunio Ohyama
    Department of Biochemistry, Faculty of Pharmacy, Tokyo University of Pharmacy and Life Science, Horinouchi, Hachioji, Tokyo, Japan
    Biol Pharm Bull 26:141-7. 2003
    ..It is concluded that IV-HA induces apoptosis in Amnion-cells, and that this apoptotic induction may be facilitated by certain sialoglycoproteins on the cell surface, and is related to changes in the intracellular redox condition...
  5. ncbi request reprint Cytotoxicity and apoptotic inducibility of Vitex agnus-castus fruit extract in cultured human normal and cancer cells and effect on growth
    Kunio Ohyama
    Department of Biochemistry, Faculty of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432 1 Horinouchi, Hachioji, Tokyo 192 0355, Japan
    Biol Pharm Bull 26:10-8. 2003
    ....
  6. ncbi request reprint Progressive apoptosis in chorion laeve trophoblast cells of human fetal membrane tissues during in vitro incubation is suppressed by antioxidative reagents
    K Ohyama
    Department of Biochemistry, Faculty of Pharmacy, Tokyo University of Pharmacy and Life Science, Tokyo, Japan
    Eur J Biochem 268:6182-9. 2001
    ..These results suggest that apoptotic cell death of the trophoblast layer of chorion tissues may be induced through intracellular oxidative stress at the stage of parturition...
  7. ncbi request reprint Biosynthesis of Vicia graminea lectin- and Vicia unijuga lectin-binding glycoproteins in human tumor and nontumor cells and an estimation of its epitope structure
    K Ohyama
    Department of Biochemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Japan
    Cancer Detect Prev 24:61-71. 2000
    ....
  8. ncbi request reprint Improvement of separation method of fragmented DNA from an apoptotic cell DNA sample for the quantitation using agarose gel electrophoresis
    K Ohyama
    Department of Biochemistry, Tokyo University of Pharmacy and Life Science, Hachioji, Japan
    Biol Pharm Bull 24:342-6. 2001
    ..The results show that DNA fragmentation rates in these cells were consistent with the electrophoretic patterns of the DNA samples shown by their photographs...
  9. doi request reprint Cytotoxic effects of flavonoids against a human colon cancer derived cell line, COLO 201: a potential natural anti-cancer substance
    Masahiko Imai
    Department of Clinical Molecular Genetics, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432 1 Horinouchi, Hachioji, Tokyo 192 0392, Japan
    Cancer Lett 276:74-80. 2009
    ..Results also implicate a potential clinical chemotherapeutic application of Vitex for the treatment of colon cancer patients...
  10. ncbi request reprint Effects of mitogen-activated protein kinase inhibitors on tumor necrosis factor-alpha gene expression and apoptosis induction in cultured human fetal membrane chorion cells infected with influenza virus
    Noboru Uchide
    Department of Clinical Molecular Genetics, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Tokyo, Japan
    Intervirology 50:99-107. 2007
    ....
  11. ncbi request reprint Effect of antioxidants on apoptosis induced by influenza virus infection: inhibition of viral gene replication and transcription with pyrrolidine dithiocarbamate
    Noboru Uchide
    Department of Biochemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432 1 Horinouchi, Hachioji, Japan
    Antiviral Res 56:207-17. 2002
    ..The synthesis of specific viral macromolecules at the early stage of infection may play a critical role in the mechanism of apoptosis induction and moderate ROS overproduction may not be involved in the mechanism...
  12. ncbi request reprint Characterization of monocyte differentiation-inducing (MDI) factors derived from human fetal membrane chorion cells undergoing apoptosis after influenza virus infection
    Noboru Uchide
    Department of Clinical Molecular Genetics, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432 1 Horinouchi, Hachioji, Tokyo 192 0392, Japan
    Int J Biochem Cell Biol 38:1926-38. 2006
    ..It is possible that these pathways represent part of the mechanism for birth complications associated with intrauterine influenza infection in pregnancy...
  13. ncbi request reprint Inhibition of influenza-virus-induced apoptosis in chorion cells of human fetal membranes by nordihydroguaiaretic Acid
    Noboru Uchide
    Department of Clinical Molecular Genetics, School of Pharmacy, Tokyo University of Pharmacy and Life Science, and Yoneyama Maternity Hospital, Tokyo, Japan
    Intervirology 48:336-40. 2005
    ..We examined effects of an antioxidant, nordihydroguaiaretic acid (NDGA), on apoptosis induction and viral proliferation. Subsequently, the results were compared with those of pyrrolidine dithiocarbamate (PDTC), another antioxidant...
  14. doi request reprint Imbalance between ROS production and elimination results in apoptosis induction in primary smooth chorion trophoblast cells prepared from human fetal membrane tissues
    Bo Yuan
    Department of Clinical Molecular Genetics, Faculty of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432 1 Horinouchi, Hachioji, Tokyo 192 0355, Japan
    Life Sci 82:623-30. 2008
    ....
  15. doi request reprint Cytotoxicity of Vitex agnus-castus fruit extract and its major component, casticin, correlates with differentiation status in leukemia cell lines
    Hidetomo Kikuchi
    Department of Clinical Molecular Genetics, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192 0392, Japan
    Int J Oncol 43:1976-84. 2013
    ..These findings provide fundamental insights into the clinical application of Vitex/casticin for hematopoietic malignancy...
  16. ncbi request reprint Differentiation of monocytes to macrophages induced by influenza virus-infected apoptotic cells
    Noboru Uchide
    Department of Biochemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432 1 Horinouchi, Hachioji, Tokyo 192 0392, Japan
    J Gen Virol 83:747-51. 2002
    ..These results suggested that monocytic THP-1 cells were morphologically and functionally differentiated to macrophages by IV-infected apoptotic cells due to a soluble factor released from the apoptotic cells...
  17. ncbi request reprint Antiviral function of pyrrolidine dithiocarbamate against influenza virus: the inhibition of viral gene replication and transcription
    Noboru Uchide
    Department of Biochemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432 1 Horinouchi, Hachioji, Tokyo 192 0392, Japan
    J Antimicrob Chemother 52:8-10. 2003
  18. doi request reprint Involvement of histone H3 phosphorylation via the activation of p38 MAPK pathway and intracellular redox status in cytotoxicity of HL-60 cells induced by Vitex agnus-castus fruit extract
    Hidetomo Kikuchi
    Department of Clinical Molecular Genetics, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Hachioji, Tokyo 192 0392, Japan
    Int J Oncol 45:843-52. 2014
    ..These results may have important implications for appropriate clinical uses of Vitex and provide novel insights into the interaction between Vitex and other conventional drugs capable of affecting intracellular redox status...
  19. doi request reprint Lactate dehydrogenase leakage as a marker for apoptotic cell degradation induced by influenza virus infection in human fetal membrane cells
    Noboru Uchide
    Department of Clinical Molecular Genetics, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, Tokyo, Japan
    Intervirology 52:164-73. 2009
    ....
  20. ncbi request reprint Semi-quantitative RT-PCR-based assay, improved by Southern hybridization technique, for polarity-specific influenza virus RNAs in cultured cells
    Noboru Uchide
    Department of Biochemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432 1 Horinouchi, Hachioji, Tokyo 192 0392, Japan
    J Virol Methods 106:125-34. 2002
    ....
  21. doi request reprint Direct contribution of inducible nitric oxide synthase expression to apoptosis induction in primary smooth chorion trophoblast cells of human fetal membrane tissues
    Bo Yuan
    Department of Clinical Molecular Genetics, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432 1 Horinouchi, Hachioji, Tokyo 192 0355, Japan
    Int J Biochem Cell Biol 41:1062-9. 2009
    ..These results suggest that apoptosis induced in the chorion trophoblast cells by the iNOS gene expression is closely linked to a physiological consequence, such as the rupture of fetal membranes...
  22. ncbi request reprint Differences in permissive cytomegalovirus infection between primary cultured human fetal membrane chorion and amnion cells
    Shigehiro Matsunaga
    Department of Clinical Molecular Genetics, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences
    Biol Pharm Bull 36:1715-21. 2013
    ..Therefore, the present study suggests that CMV may replicate rapidly in the chorion cell layer and slowly in the amnion cell layer during intrauterine infection. ..
  23. ncbi request reprint Contribution of inducible nitric oxide synthase and cyclooxygenase-2 to apoptosis induction in smooth chorion trophoblast cells of human fetal membrane tissues
    Bo Yuan
    Department of Clinical Molecular Genetics, Faculty of Pharmacy, Tokyo University of Pharmacy and Life Science, 1432 1 Horinouchi, Hachioji, Tokyo 192 0355, Japan
    Biochem Biophys Res Commun 341:822-7. 2006
    ....
  24. ncbi request reprint Induction of pro-inflammatory cytokine gene expression and apoptosis in human chorion cells of fetal membranes by influenza virus infection: possible implications for maintenance and interruption of pregnancy during infection
    Noboru Uchide
    Department of Clinical Molecular Genetics, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Tokyo, Japan
    Med Sci Monit 11:RA7-16. 2005
    ..Understanding the interactions would make important contributions to the elucidation of the pathogenesis of influenza virus infection during pregnancy...
  25. ncbi request reprint Differential mRNA expression of inflammatory cytokines in cultured human fetal membrane cells responding to influenza virus infection
    Noboru Uchide
    Department of Biochemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Japan
    Biol Pharm Bull 25:239-43. 2002
    ....
  26. pmc Possible roles of proinflammatory and chemoattractive cytokines produced by human fetal membrane cells in the pathology of adverse pregnancy outcomes associated with influenza virus infection
    Noboru Uchide
    Department of Clinical Molecular Genetics, School of Pharmacy, Tokyo University of Pharmacy and Life Sciences, 1432 1 Horinouchi, Hachioji, Tokyo 192 0392, Japan
    Mediators Inflamm 2012:270670. 2012
    ....
  27. ncbi request reprint Apoptosis in cultured human fetal membrane cells infected with influenza virus
    Noboru Uchide
    Department of Biochemistry, School of Pharmacy, Tokyo University of Pharmacy and Life Science, Hachioji, Japan
    Biol Pharm Bull 25:109-14. 2002
    ..We further observed that influenza virus replication is associated with the induction of apoptosis...
  28. doi request reprint Sucutiniranes A and B, new cassane-type diterpenes from Bowdichia nitida
    Yosuke Matsuno
    Faculty of Pharmaceutical Sciences, Hoshi University, Ebara 2 4 41, Shinagawa ku, Tokyo 142 8501, Japan
    Bioorg Med Chem Lett 18:3774-7. 2008
    ..Sucutinirane A (1) and 3 showed a moderate cytotoxicity against human colon carcinoma COLO201 cells, and 6alpha,7beta-diacetoxyvouacapane (4) showed in vitro antiplasmodial activity against parasite Plasmodium falciparum 3D7...