Kiyoshi Kita

Summary

Affiliation: University of Tokyo
Country: Japan

Publications

  1. ncbi request reprint Role of complex II in anaerobic respiration of the parasite mitochondria from Ascaris suum and Plasmodium falciparum
    Kiyoshi Kita
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, 113 0033, Tokyo, Japan
    Biochim Biophys Acta 1553:123-39. 2002
  2. ncbi request reprint [Adaptation to low oxygen tension in parasite mitochondria]
    Kita Kiyoshi
    Tanpakushitsu Kakusan Koso 47:37-44. 2002
  3. ncbi request reprint Parasite mitochondria as drug target: diversity and dynamic changes during the life cycle
    Kiyoshi Kita
    Department of Biomedical Chemistry, Graduate School of Medicine, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Curr Med Chem 10:2535-48. 2003
  4. pmc Novel type of linear mitochondrial genomes with dual flip-flop inversion system in apicomplexan parasites, Babesia microti and Babesia rodhaini
    Kenji Hikosaka
    Laboratory of Malariology, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    BMC Genomics 13:622. 2012
  5. pmc Adherence to antiretroviral therapy (ART) during the early months of treatment in rural Zambia: influence of demographic characteristics and social surroundings of patients
    Yuri Sasaki
    Department of Infection Control and Prevention, Graduate School of Nursing, Nagoya City University, Kawasumi 1, Mizuho ku, Nagoya shi, Aichi, 467 8601, Japan
    Ann Clin Microbiol Antimicrob 11:34. 2012
  6. pmc Gain and loss of an intron in a protein-coding gene in Archaea: the case of an archaeal RNA pseudouridine synthase gene
    Shin ichi Yokobori
    Department of Molecular Biology, School of Life Science, Tokyo University of Pharmacy and Life Science, Horinouchi, Hachioji, Tokyo 192 0392, Japan
    BMC Evol Biol 9:198. 2009
  7. doi request reprint Purification and kinetic characterization of recombinant alternative oxidase from Trypanosoma brucei brucei
    Yasutoshi Kido
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Bunkyo ku, Tokyo, Japan
    Biochim Biophys Acta 1797:443-50. 2010
  8. doi request reprint Siccanin rediscovered as a species-selective succinate dehydrogenase inhibitor
    Tatsushi Mogi
    Department of Biomedical Chemistry, The University of Tokyo, Bunkyo ku, Tokyo 113 0033, Japan
    J Biochem 146:383-7. 2009
  9. doi request reprint Biochemical characterization of highly active Trypanosoma brucei gambiense glycerol kinase, a promising drug target
    Emmanuel Oluwadare Balogun
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Biochem 154:77-84. 2013
  10. doi request reprint Antibiotics LL-Z1272 identified as novel inhibitors discriminating bacterial and mitochondrial quinol oxidases
    Tatsushi Mogi
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Biochim Biophys Acta 1787:129-33. 2009

Detail Information

Publications89

  1. ncbi request reprint Role of complex II in anaerobic respiration of the parasite mitochondria from Ascaris suum and Plasmodium falciparum
    Kiyoshi Kita
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, 113 0033, Tokyo, Japan
    Biochim Biophys Acta 1553:123-39. 2002
    ..Recent research has shown that the mitochondrial complex II plays an important role in the anaerobic energy metabolism of parasites inhabiting hosts, by acting as quinol-fumarate reductase...
  2. ncbi request reprint [Adaptation to low oxygen tension in parasite mitochondria]
    Kita Kiyoshi
    Tanpakushitsu Kakusan Koso 47:37-44. 2002
  3. ncbi request reprint Parasite mitochondria as drug target: diversity and dynamic changes during the life cycle
    Kiyoshi Kita
    Department of Biomedical Chemistry, Graduate School of Medicine, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Curr Med Chem 10:2535-48. 2003
    ..In fact, a specific inhibitor of nematode complex I, nafuredin, has been found in mass-screening using parasite mitochondria...
  4. pmc Novel type of linear mitochondrial genomes with dual flip-flop inversion system in apicomplexan parasites, Babesia microti and Babesia rodhaini
    Kenji Hikosaka
    Laboratory of Malariology, Research Institute for Microbial Diseases, Osaka University, 3 1 Yamadaoka, Suita, Osaka 565 0871, Japan
    BMC Genomics 13:622. 2012
    ..We are interested in the evolutionary origin of linear mt genomes of Babesia/Theileria, and have investigated mt genome structures in members of archaeopiroplasmid, a lineage branched off earlier from Babesia/Theileria...
  5. pmc Adherence to antiretroviral therapy (ART) during the early months of treatment in rural Zambia: influence of demographic characteristics and social surroundings of patients
    Yuri Sasaki
    Department of Infection Control and Prevention, Graduate School of Nursing, Nagoya City University, Kawasumi 1, Mizuho ku, Nagoya shi, Aichi, 467 8601, Japan
    Ann Clin Microbiol Antimicrob 11:34. 2012
    ..However, sustaining high levels of adherence to ART is a challenge. This study aimed to identify the predictive factors associated with ART adherence during the early months of treatment in rural Zambia...
  6. pmc Gain and loss of an intron in a protein-coding gene in Archaea: the case of an archaeal RNA pseudouridine synthase gene
    Shin ichi Yokobori
    Department of Molecular Biology, School of Life Science, Tokyo University of Pharmacy and Life Science, Horinouchi, Hachioji, Tokyo 192 0392, Japan
    BMC Evol Biol 9:198. 2009
    ..However, the exact timing of the intron insertion was not determined and verification of the putative secondary loss of the intron in some lineages was not performed...
  7. doi request reprint Purification and kinetic characterization of recombinant alternative oxidase from Trypanosoma brucei brucei
    Yasutoshi Kido
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Bunkyo ku, Tokyo, Japan
    Biochim Biophys Acta 1797:443-50. 2010
    ..The specific inhibitor, ascofuranone, inhibited the enzyme in a mixed-type inhibition manner with respect to ubiquinol-1...
  8. doi request reprint Siccanin rediscovered as a species-selective succinate dehydrogenase inhibitor
    Tatsushi Mogi
    Department of Biomedical Chemistry, The University of Tokyo, Bunkyo ku, Tokyo 113 0033, Japan
    J Biochem 146:383-7. 2009
    ..Species-selective inhibition by siccanin is unique among succinate dehydrogenase inhibitors, and thus siccanin is a potential lead compound for new chemotherapeutics...
  9. doi request reprint Biochemical characterization of highly active Trypanosoma brucei gambiense glycerol kinase, a promising drug target
    Emmanuel Oluwadare Balogun
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Biochem 154:77-84. 2013
    ..Using this stabilized protein, crystals of trypanosome GK with better resolution were obtained. This will accelerate the success of GK inhibitor development for drug design. ..
  10. doi request reprint Antibiotics LL-Z1272 identified as novel inhibitors discriminating bacterial and mitochondrial quinol oxidases
    Tatsushi Mogi
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Biochim Biophys Acta 1787:129-33. 2009
    ....
  11. doi request reprint Identification of new inhibitors for alternative NADH dehydrogenase (NDH-II)
    Tatsushi Mogi
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
    FEMS Microbiol Lett 291:157-61. 2009
    ..oxydans NDH-II. Further, we examined their effects on Mycobacterium smegmatis and Plasmodium yoelii NDH-II as model pathogen enzymes...
  12. pmc Expression, purification and crystallization of Trypanosoma cruzi dihydroorotate dehydrogenase complexed with orotate
    Daniel Ken Inaoka
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, 7 3 1 Hongo, Tokyo 113 0033, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 61:875-8. 2005
    ..87, b = 71.89, c = 123.27 A. The presence of two molecules in the asymmetric unit (2 x 34 kDa) gives a crystal volume per protein weight (VM) of 2.2 A3 Da(-1) and a solvent content of 44%...
  13. doi request reprint Trypanosome alternative oxidase, a potential therapeutic target for sleeping sickness, is conserved among Trypanosoma brucei subspecies
    Kosuke Nakamura
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, 7 3 1, Hongo, Tokyo 113 0033, Japan
    Parasitol Int 59:560-4. 2010
    ..brucei subspecies in search of a cure for HAT. Our in vitro study using T. b. rhodesiense confirmed the effectiveness of ascofuranone (IC(50) value: 1 nM) to eliminate trypanosomes in human infective strain cultures...
  14. ncbi request reprint Isolation and characterization of the stage-specific cytochrome b small subunit (CybS) of Ascaris suum complex II from the aerobic respiratory chain of larval mitochondria
    Hisako Amino
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
    Mol Biochem Parasitol 128:175-86. 2003
    ..Such RQFR activity of the larval complex II would be essential for rapid adaptation to the dramatic change of oxygen availability during infection of the host...
  15. ncbi request reprint Overproduction of highly active trypanosome alternative oxidase in Escherichia coli heme-deficient mutant
    Yoshihisa Fukai
    Department of Biomedical Chemistry, Graduate School of Medicine, University of Tokyo 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Parasitol Int 52:237-41. 2003
    ....
  16. pmc Novel mitochondrial complex II isolated from Trypanosoma cruzi is composed of 12 peptides including a heterodimeric Ip subunit
    Jorge Morales
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 0033, USA
    J Biol Chem 284:7255-63. 2009
    ..In addition, the enzyme binds protoheme IX, but SDH3 lacks a ligand histidine. These unusual features are unique in the Trypanosomatida and make their Complex II a target for new chemotherapeutic agents...
  17. doi request reprint Structures of Trypanosoma cruzi dihydroorotate dehydrogenase complexed with substrates and products: atomic resolution insights into mechanisms of dihydroorotate oxidation and fumarate reduction
    Daniel Ken Inaoka
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Tokyo 113 0033, Japan
    Biochemistry 47:10881-91. 2008
    ....
  18. pmc Structure of the trypanosome cyanide-insensitive alternative oxidase
    Tomoo Shiba
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Tokyo 113 0033, Japan
    Proc Natl Acad Sci U S A 110:4580-5. 2013
    ..A second cavity interacts with the inhibitor-binding cavity at the diiron center. We suggest that both cavities bind ubiquinol and along with Tyr220 are required for the catalytic cycle for O2 reduction...
  19. ncbi request reprint Purification of active recombinant trypanosome alternative oxidase
    Coichi Nihei
    Department of Biomedical Chemistry, Graduate School of Medicine, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    FEBS Lett 538:35-40. 2003
    ..Kinetic analysis of the purified TAO revealed that the specific inhibitor ascofuranone is a competitive inhibitor of ubiquinol oxidase activity...
  20. pmc Crystallization of mitochondrial rhodoquinol-fumarate reductase from the parasitic nematode Ascaris suum with the specific inhibitor flutolanil
    Arihiro Osanai
    Department of Biomedical Chemistry, Graduate School of Medicine, University of Tokyo, Tokyo, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 65:941-4. 2009
    ..75, b = 129.08, c = 221.12 A, and diffracted to 2.8 A resolution using synchrotron radiation. The presence of two molecules in the asymmetric unit (120 kDa x 2) gives a crystal volume per protein mass (V(M)) of 3.6 A(3) Da(-1)...
  21. doi request reprint A conserved lysine residue in the crenarchaea-specific loop is important for the crenarchaeal splicing endonuclease activity
    Maho Okuda
    Department of Biomedical Chemistry, University of Tokyo, Tokyo 113 0033, Japan
    J Mol Biol 405:92-104. 2011
    ....
  22. doi request reprint Polymyxin B identified as an inhibitor of alternative NADH dehydrogenase and malate: quinone oxidoreductase from the Gram-positive bacterium Mycobacterium smegmatis
    Tatsushi Mogi
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Biochem 146:491-9. 2009
    ..Screening of the library with bacterial respiratory enzymes resulted in unprecedented findings, so we are hoping that continuing efforts could identify lead compounds for new drugs targeting to mycobacterial respiratory enzymes...
  23. doi request reprint Crystal structure of mitochondrial quinol-fumarate reductase from the parasitic nematode Ascaris suum
    Hironari Shimizu
    Department of Biomedical Chemistry, Graduate School of Medicine, University of Tokyo, Tokyo, Japan
    J Biochem 151:589-92. 2012
    ..suum enzyme. We discuss herein the structure-function relationship of the enzyme and the critical role of the low redox potential of rhodoquinol in the fumarate reduction of A. suum complex II...
  24. pmc Screening of detergents for solubilization, purification and crystallization of membrane proteins: a case study on succinate:ubiquinone oxidoreductase from Escherichia coli
    Hironari Shimizu
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 64:858-62. 2008
    ..Crystallization took place before detergent phase separation occurred and the type of detergent mixture affected the crystal form...
  25. pmc Functional importance of crenarchaea-specific extra-loop revealed by an X-ray structure of a heterotetrameric crenarchaeal splicing endonuclease
    Shigeo Yoshinari
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Nucleic Acids Res 37:4787-98. 2009
    ..Rather, the structural basis for the broad substrate specificity is built into the crenarchaeal splicing endonuclease itself...
  26. ncbi request reprint Mutational analysis of the Trypanosoma vivax alternative oxidase: the E(X)6Y motif is conserved in both mitochondrial alternative oxidase and plastid terminal oxidase and is indispensable for enzyme activity
    Kosuke Nakamura
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Tokyo 113 0033, Japan
    Biochem Biophys Res Commun 334:593-600. 2005
    ..Based on these findings, it appears that AOXs and PTOXs are a novel subclass of diiron carboxylate proteins that require the conserved motif E(X)(6)Y for enzyme activity...
  27. ncbi request reprint Change of subunit composition of mitochondrial complex II (succinate-ubiquinone reductase/quinol-fumarate reductase) in Ascaris suum during the migration in the experimental host
    Fumiko Iwata
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Parasitol Int 57:54-61. 2008
    ..This clearly indicates that the rearrangement of complex II begins with a change in the isoform of the anchor CybS subunit, followed by a similar change in the Fp subunit...
  28. doi request reprint Critical importance of the de novo pyrimidine biosynthesis pathway for Trypanosoma cruzi growth in the mammalian host cell cytoplasm
    Muneaki Hashimoto
    Department of Molecular and Cellular Parasitology, Juntendo University School of Medicine, Bunkyo ku, Tokyo, Japan
    Biochem Biophys Res Commun 417:1002-6. 2012
    ..Thus, the de novo pyrimidine pathway is important for proliferation of T. cruzi in the host cell cytoplasm and represents a promising target for chemotherapy against Chagas disease...
  29. ncbi request reprint Genetic diversity and kinetic properties of Trypanosoma cruzi dihydroorotate dehydrogenase isoforms
    Idalia Sariego
    Department of Molecular and Cellular Parasitology, Juntendo University School of Medicine, Hongo 2 1 1, Bunkyo ku, Tokyo 113 8421, Japan
    Parasitol Int 55:11-6. 2006
    ..cruziDHODs are conserved. Our findings facilitate further exploitation of T. cruzi DHOD inhibitors, as chemotherapeutic agents against Chagas' disease...
  30. doi request reprint Divergence of the mitochondrial genome structure in the apicomplexan parasites, Babesia and Theileria
    Kenji Hikosaka
    Laboratory of Malariology, International Research Center of Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
    Mol Biol Evol 27:1107-16. 2010
    ..These results suggest that the Theileria mt genome is highly diverse with lineage-specific evolution in two Theileria species: genome inversion in T. orientalis and gene-embedded long TIR in T. equi...
  31. doi request reprint Critical roles of the mitochondrial complex II in oocyst formation of rodent malaria parasite Plasmodium berghei
    Akina Hino
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    J Biochem 152:259-68. 2012
    ..These results suggest that malaria parasite may switch the energy metabolism from glycolysis to oxidative phosphorylation to adapt to the insect vector where glucose is not readily available for ATP production...
  32. doi request reprint Toward understanding the role of mitochondrial complex II in the intraerythrocytic stages of Plasmodium falciparum: gene targeting of the Fp subunit
    Takeshi Q Tanaka
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Parasitol Int 61:726-8. 2012
    ..This indicates that complex II functions as a quinol-fumarate reductase (QFR) to form succinate from fumarate in the intraerythrocytic parasite...
  33. pmc Overproduction, purification, crystallization and preliminary X-ray diffraction analysis of Trypanosoma brucei gambiense glycerol kinase
    Emmanuel Oluwadare Balogun
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 66:304-8. 2010
    ..84, b = 121.50, c = 154.59 A. The presence of two rTbgGK molecules in the asymmetric unit gives a Matthews coefficient (V(M)) of 2.5 A(3) Da(-1), corresponding to 50% solvent content...
  34. doi request reprint Identification of mitochondrial Complex II subunits SDH3 and SDH4 and ATP synthase subunits a and b in Plasmodium spp
    Tatsushi Mogi
    Department of Biomedical Chemistry, The University of Tokyo, Hongo, Bunkyo ku, Japan
    Mitochondrion 9:443-53. 2009
    ..Transmembrane helix IV of ATP synthase subunit a lacks an essential Arg residue. Membrane anchors of Plasmodium Complex II and ATP synthase are divergent from orthologs and promising targets for new chemotherapeutics...
  35. doi request reprint Contribution of the FAD and quinone binding sites to the production of reactive oxygen species from Ascaris suum mitochondrial complex II
    Madhavi P Paranagama
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Mitochondrion 10:158-65. 2010
    ..suum adult worm together with the absence of complexes III and IV activities in its respiratory chain, it is a good model to examine the reactive oxygen species production from the mitochondrial complex II...
  36. doi request reprint Gramicidin S identified as a potent inhibitor for cytochrome bd-type quinol oxidase
    Tatsushi Mogi
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Bunkyo ku, Tokyo, Japan
    FEBS Lett 582:2299-302. 2008
    ..Our findings would provide a new insight into the development of gramicidin S analogs, which do not share the target and mechanism with conventional antibiotics...
  37. ncbi request reprint Complex II from phototrophic purple bacterium Rhodoferax fermentans displays rhodoquinol-fumarate reductase activity
    Hiroko Miyadera
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Japan
    Eur J Biochem 270:1863-74. 2003
    ..The results strongly indicate that R. fermentans complex II and mitochondrial QFR might have evolved independently, although they both utilize RQ for fumarate reduction...
  38. pmc In vivo curative and protective potential of orally administered 5-aminolevulinic acid plus ferrous ion against malaria
    Shigeo Suzuki
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan SBI Pharmaceuticals Co, Ltd, Tokyo, Japan
    Antimicrob Agents Chemother 59:6960-7. 2015
    ..These findings provide clear evidence that ALA/SFC is effective in an experimental animal model of malaria and may facilitate the development of a new class of antimalarial drug. ..
  39. doi request reprint Molecular basis for the reverse reaction of African human trypanosomes glycerol kinase
    Emmanuel Oluwadare Balogun
    Department of Applied Biology, Graduate School of Science and Technology, Kyoto Institute of Technology, Sakyo ku, Kyoto, 606 8585, Japan Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo, 113 0033, Japan Department of Biochemistry, Ahmadu Bello University, Zaria, 2222, Nigeria
    Mol Microbiol 94:1315-29. 2014
    ..Together, they provide an encouraging molecular framework for the development of trypanosome GK-specific inhibitors, which may lead to the design of new and safer trypanocidal drug(s)...
  40. doi request reprint A bacterial elongation factor G homologue exclusively functions in ribosome recycling in the spirochaete Borrelia burgdorferi
    Takuma Suematsu
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Tokyo, Japan
    Mol Microbiol 75:1445-54. 2010
    ..These results indicate that two B. burgdorferi EF-G paralogues are close relatives to mitochondrial EF-G paralogues rather than the conventional bacterial EF-G, in both their phylogenetic and biochemical features...
  41. ncbi request reprint Strain-specific difference in amino acid sequences of trypanosome alternative oxidase
    Yoshihisa Fukai
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Parasitol Int 51:195-9. 2002
    ..In this study, we found amino acid sequence of the C-terminal part of TAO from the strain that we are using, T. b. brucei TC221, is considerably different from that of the EATRO110 strain...
  42. ncbi request reprint Direct evidence for expression of type II flavoprotein subunit in human complex II (succinate-ubiquinone reductase)
    Eriko Tomitsuka
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Biochem Biophys Res Commun 311:774-9. 2003
    ....
  43. ncbi request reprint Decursin and decursinol angelate selectively inhibit NADH-fumarate reductase of Ascaris suum
    Kazuro Shiomi
    Kitasato Institute for Life Sciences, Kitasato University, Tokyo, Japan
    Planta Med 73:1478-81. 2007
    ..In contrast, decursinol angelate moderately inhibits bovine heart complexes II and III. Decursinol inhibits A. SUUM NFRD to a similar extent, but its target is complex II. It also inhibits bovine heart complexes II and III...
  44. doi request reprint Cloning and characterization of hypoxia-inducible factor-1 subunits from Ascaris suum - a parasitic nematode highly adapted to changes of oxygen conditions during its life cycle
    Miho Goto
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Gene 516:39-47. 2013
    ....
  45. pmc Autophagy-related Atg8 localizes to the apicoplast of the human malaria parasite Plasmodium falciparum
    Kei Kitamura
    Department of Physiology and Cell Biology, Tokyo Medical and Dental University, Tokyo, Japan
    PLoS ONE 7:e42977. 2012
    ..These data suggest that, although Plasmodium parasites have lost most Atg proteins during evolution, they use the Atg8 conjugation system for the unique organelle, the apicoplast...
  46. doi request reprint Molecular interaction of the first 3 enzymes of the de novo pyrimidine biosynthetic pathway of Trypanosoma cruzi
    Takeshi Nara
    Department of Molecular and Cellular Parasitology, Juntendo University Graduate School of Medicine, 2 1 1 Hongo, Bunkyo ku, Tokyo 113 8421, Japan
    Biochem Biophys Res Commun 418:140-3. 2012
    ....
  47. ncbi request reprint Parasite mitochondria as a target of chemotherapy: inhibitory effect of licochalcone A on the Plasmodium falciparum respiratory chain
    Fumika Mi-ichi
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Ann N Y Acad Sci 1056:46-54. 2005
    ..Because the property of the P. falciparum bc1 complex is different from that of the mammalian host, chalcones would be a promising candidate for a new antimalarial drug...
  48. pmc Crystallization and preliminary crystallographic analysis of cyanide-insensitive alternative oxidase from Trypanosoma brucei brucei
    Yasutoshi Kido
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Tokyo 113 0033, Japan
    Acta Crystallogr Sect F Struct Biol Cryst Commun 66:275-8. 2010
    ..2 A(3) Da(-1), which corresponds to a solvent content of 61.0%. This is the first report of a crystal of the membrane-bound diiron proteins, which include AOXs...
  49. ncbi request reprint Identification and characterization of amino acid residues essential for the active site of UDP-N-acetylenolpyruvylglucosamine reductase (MurB) from Staphylococcus aureus
    Satoshi Nishida
    Laboratory of Developmental Biochemistry, Graduate School of Pharmaceutical Sciences, University of Tokyo, Japan
    J Biol Chem 281:1714-24. 2006
    ..The results presented here identify for the first time the amino acid residues of MurB that are required for the interaction with UDP-Glc-NAcEP and NADP+...
  50. pmc Atpenins, potent and specific inhibitors of mitochondrial complex II (succinate-ubiquinone oxidoreductase)
    Hiroko Miyadera
    Department of Biomedical Chemistry, Graduate School of Medicine, University of Tokyo, Bunkyo ku, Tokyo 113 0033, Japan
    Proc Natl Acad Sci U S A 100:473-7. 2003
    ..Therefore, atpenins may be useful tools for clarifying the biochemical and structural properties of complex II, as well as for determining its physiological roles in mammalian tissues...
  51. ncbi request reprint Paecilaminol, a new NADH-fumarate reductase inhibitor, produced by Paecilomyces sp. FKI-0550
    Hideaki Ui
    Kitasato Institute for Life Sciences and Graduate School of Infection Control Sciences, Kitasato University, 5 9 1 Shirokane, Minato ku, Tokyo, Japan
    J Antibiot (Tokyo) 59:591-6. 2006
    ..FKI-0550. It is an amino alcohol compound, the structure being established as 2-amino-14,16-dimethyl-3-octadecanol. Paecilaminol exhibited an IC50 value of 5.1 microM against Ascaris suum NADH-fumarate reductase...
  52. ncbi request reprint Cloning and characterization of ferredoxin and ferredoxin-NADP+ reductase from human malaria parasite
    Yoko Kimata-Ariga
    Institute for Protein Research, Osaka University, 3 2 Yamadaoka, Suita, Japan
    J Biochem 141:421-8. 2007
    ..falciparum Fd in a reconstituted system of NADPH-dependent cytochrome c reduction. These results indicate that an NADPH-FNR-Fd cascade is operative in the apicoplast of human malaria parasites...
  53. doi request reprint Mitochondrial fumarate reductase as a target of chemotherapy: from parasites to cancer cells
    Chika Sakai
    Department of Biomedical Chemistry, The University of Tokyo, Tokyo, Japan
    Biochim Biophys Acta 1820:643-51. 2012
    ..Role of isoforms of human complex II in the hypoxic condition of cancer cells and fetal tissues is a challenge. This article is part of a Special Issue entitled Biochemistry of Mitochondria, Life and Intervention 2010...
  54. ncbi request reprint Electron-transfer complexes in Ascaris mitochondria
    Kiyoshi Kita
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Japan
    Adv Parasitol 51:95-131. 2002
    ....
  55. doi request reprint Biochemical and spectroscopic properties of cyanide-insensitive quinol oxidase from Gluconobacter oxydans
    Tatsushi Mogi
    Department of Biomedical Chemistry, The University of Tokyo, Bunkyo ku, Japan
    J Biochem 146:263-71. 2009
    ..We found also a broad low-spin signal at g = 3.2, attributable to heme b(558). Further, we identified the presence of heme D by mass spectrometry. In conclusion, CioAB binds all three ham species present in cytochrome bd quinol oxidase...
  56. ncbi request reprint Direct evidence for two distinct forms of the flavoprotein subunit of human mitochondrial complex II (succinate-ubiquinone reductase)
    Eriko Tomitsuka
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033
    J Biochem 134:191-5. 2003
    ..Interestingly, one of the Fp isoforms was encoded as an intronless gene...
  57. doi request reprint Mitochondrial dehydrogenases in the aerobic respiratory chain of the rodent malaria parasite Plasmodium yoelii yoelii
    Kenji Kawahara
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Hongo, Bunkyo ku, Tokyo, Japan
    J Biochem 145:229-37. 2009
    ..Our findings are consistent with the notion that rodent malaria mitochondria are fully capable of oxidative phosphorylation and that these mitochondrial enzymes are potential targets for new antiplasmodials...
  58. doi request reprint Highly conserved gene arrangement of the mitochondrial genomes of 23 Plasmodium species
    Kenji Hikosaka
    Laboratory of Malariology, International Research Center of Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
    Parasitol Int 60:175-80. 2011
    ..Additionally, we found 22-25 bp-inverted repeat sequences, which may be involved in the generation of lineage-specific mt genome arrangements after divergence from a common ancestor of the genera Eimeria and Plasmodium/Leucocytozoon...
  59. ncbi request reprint Rhodoquinone reaction site of mitochondrial complex I, in parasitic helminth, Ascaris suum
    Tetsuo Yamashita
    Department of Biomedical Chemistry, Graduate School of Medicine, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Biochim Biophys Acta 1608:97-103. 2004
    ..4 and 7.2. Our results indicate that although A. suum complex I uses both RQ(2) and UQ(2) as an electron acceptor, the manner of reaction (or binding) of the two quinones differs...
  60. doi request reprint Identification of an entire set of tRNA molecules and characterization of cleavage sites of the intron-containing tRNA precursors in acidothermophilic crenarchaeon Sulfolobus tokodaii strain7
    Syuji Yamazaki
    National Institute of Technology and Evaluation, Shibuya ku, Tokyo, Japan
    Gene 489:103-10. 2011
    ..This is the first report to empirically determine the actual cleavage and splice sites of introns in the whole set of archaeal tRNA genes, and reassigns the exon-intron borders with a novel and more plausible non-canonical BHB structure...
  61. doi request reprint Type II Fp of human mitochondrial respiratory complex II and its role in adaptation to hypoxia and nutrition-deprived conditions
    Chika Sakai
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Tokyo 113 0033, Japan
    Mitochondrion 13:602-9. 2013
    ..The result suggests complex II with type II Fp may function in cells with low mitochondrial matrix pH caused by ischemia and its function is related to cellular adaptation to ischemia. ..
  62. ncbi request reprint Trypanosome alternative oxidase as a target of chemotherapy
    Coichi Nihei
    Department of Biomedical Chemistry, Graduate School of Medicine, University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo, Japan
    Biochim Biophys Acta 1587:234-9. 2002
    ..Recently, we found the most potent inhibitor of TAO to date, ascofuranone, a compound isolated from the phytopathogenic fungus, Ascochyta visiae...
  63. doi request reprint Concatenated mitochondrial DNA of the coccidian parasite Eimeria tenella
    Kenji Hikosaka
    International Research Center of Infectious Diseases, Research Institute for Microbial Diseases, Osaka University, Suita, Osaka, Japan
    Mitochondrion 11:273-8. 2011
    ..tenella mt genome was concatemeric with similar protein-coding genes and rRNA gene fragments to Plasmodium. Copy number was 50-fold of the nuclear genome. Evolution of structural divergence in the apicomplexan mt genomes is discussed...
  64. doi request reprint Design and synthesis of potent substrate-based inhibitors of the Trypanosoma cruzi dihydroorotate dehydrogenase
    Daniel Ken Inaoka
    School of Tropical Medicine and Global Health, Nagasaki University, Nagasaki 852 8523, Japan Graduate School of Medicine, The University of Tokyo, Tokyo 113 0033, Japan
    Bioorg Med Chem . 2017
    ..The information presented here will be invaluable in the search for next-generation drug leads for Chagas disease...
  65. ncbi request reprint Mitochondria and apicoplast of Plasmodium falciparum: behaviour on subcellular fractionation and the implication
    Tamaki Kobayashi
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, 7 3 1 Hongo, Tokyo 113 0033, Japan
    Mitochondrion 7:125-32. 2007
    ..This implies these two plasmodial organelles are bound each other. This is the first experimental evidence of a physical binding between the two organelles in Plasmodium...
  66. ncbi request reprint Archaeal pre-mRNA splicing: a connection to hetero-oligomeric splicing endonuclease
    Shigeo Yoshinari
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Biochem Biophys Res Commun 346:1024-32. 2006
    ..tokodaii. These observations are consistent with previous reports indicating that subunit composition of the splicing endonuclease contributes to substrate specificity...
  67. pmc Plasmodium cynomolgi genome sequences provide insight into Plasmodium vivax and the monkey malaria clade
    Shin Ichiro Tachibana
    Laboratory of Malariology, Research Institute for Microbial Diseases, Osaka University, Suita, Japan
    Nat Genet 44:1051-5. 2012
    ..The sequencing of the P. cynomolgi genome is a critical step in developing a model system for P. vivax research and in counteracting the neglect of P. vivax...
  68. ncbi request reprint Advances in drug discovery and biochemical studies
    Kiyoshi Kita
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Tokyo 113 0033, Japan
    Trends Parasitol 23:223-9. 2007
    ..Intensive studies of parasite energy metabolism, such as NADH-fumarate reductase systems and the synthetic pathways of nucleic acids and amino acids, also contribute to the identification of novel and unique drug targets...
  69. doi request reprint Diversity in mitochondrial metabolic pathways in parasitic protists Plasmodium and Cryptosporidium
    Tatsushi Mogi
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Bunkyo ku, Tokyo, Japan
    Parasitol Int 59:305-12. 2010
    ..We hope that our findings will help in understanding the apicomplexan metabolism and development of new chemotherapeutics with novel targets...
  70. ncbi request reprint Unique properties of respiratory chain in Plasmodium falciparum mitochondria
    Fumika Mi-ichi
    Dept of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Hongo Bunkyo ku, Tokyo 113 0033, Japan
    Adv Exp Med Biol 531:117-33. 2003
  71. pmc Pharmacophore modeling for anti-Chagas drug design using the fragment molecular orbital method
    Ryunosuke Yoshino
    Global Scientific Information and Computing Center, Tokyo Institute of Technology, Meguro, Tokyo, 152 8550, Japan Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo, Tokyo, 113 8657, Japan
    PLoS ONE 10:e0125829. 2015
    ..cruzi dihydroorotate dehydrogenase (TcDHODH) by fragment molecular orbital (FMO) calculation for orotate, oxonate, and 43 orotate derivatives...
  72. ncbi request reprint Complementation of Escherichia coli ubiF mutation by Caenorhabditis elegans CLK-1, a product of the longevity gene of the nematode worm
    Akihiko Adachi
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Hongo, Bunkyo ku, 113 0033, Tokyo, Japan
    FEBS Lett 543:174-8. 2003
    ..coli ubiF mutant strain and rescued the ability to synthesize ubiquinone, suggesting that the eukaryotic CLK-1/Coq7p family could function as bacterial UbiF...
  73. ncbi request reprint Isolation and physiochemical properties of protein-rich nematode mitochondrial ribosomes
    Feng Zhao
    Department of Integrated Biosciences, University of Tokyo, 5 1 5 Kashiwanoha, Kashiwa, Chiba 277 8562, Japan
    Biochemistry 44:9232-7. 2005
    ..Though the nematode mitoribosome has a larger size than the bacterial ribosome, it does not differ significantly in size from mammalian mitoribosomes...
  74. doi request reprint Gramicidin S and polymyxins: the revival of cationic cyclic peptide antibiotics
    Tatsushi Mogi
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Bunkyo ku, Tokyo 113 0033, Japan
    Cell Mol Life Sci 66:3821-6. 2009
    ..Improvement of the toxicity and optimization of the structures and clinical uses are urgently needed for their effective application in combating drug-resistant bacteria...
  75. ncbi request reprint Relationship between reactive oxygen species and heme metabolism during the differentiation of Neuro2a cells
    Noriko Shinjyo
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Biochem Biophys Res Commun 358:130-5. 2007
    ..These results suggest that heme biosynthesis following the degradation of heme protects Neuro2a cells from oxidative stress caused by ROS during differentiation...
  76. ncbi request reprint Introns in protein-coding genes in Archaea
    Yoh ichi Watanabe
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    FEBS Lett 510:27-30. 2002
    ....
  77. ncbi request reprint [mtDNA of parasites]
    Yoh ichi Watanabe
    Tanpakushitsu Kakusan Koso 50:1817-21. 2005
  78. doi request reprint Age-related changes in the activities of respiratory chain complexes and mitochondrial morphology in Drosophila
    Yukiko Oda
    Department of Molecular Biology and Biochemistry, Ochanomizu University, Otsuka, Bunkyo ku, Tokyo 112 8610, Japan
    Mitochondrion 12:345-51. 2012
    ..These changes had already appeared before the survival began to decrease, clearly indicating that the accumulation of such changes seriously damages mitochondrial function...
  79. doi request reprint Isolation and Caenorhabditis elegans lifespan assay of flavonoids from onion
    You Lin Xue
    Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1 1 1 Yayoi, Bunkyo ku, Tokyo 113 8657, Japan
    J Agric Food Chem 59:5927-34. 2011
    ..Quercetin showed the highest antioxidative activity, whereas Q3M showed the strongest antiaging activity among these flavonoids, which might be related to its high hydrophilicity...
  80. ncbi request reprint Up-regulation of heme biosynthesis during differentiation of Neuro2a cells
    Noriko Shinjyo
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033
    J Biochem 139:373-81. 2006
    ..This study suggests the up-regulation of heme biosynthesis and differential regulation of the heme a, b, and c levels during neuronal differentiation...
  81. doi request reprint A cryptic algal group unveiled: a plastid biosynthesis pathway in the oyster parasite Perkinsus marinus
    Motomichi Matsuzaki
    Department of Biological Sciences, Graduate School of Science, University of Tokyo, Tokyo, Japan
    Mol Biol Evol 25:1167-79. 2008
    ....
  82. ncbi request reprint Functional reconstitution of a crenarchaeal splicing endonuclease in vitro
    Shigeo Yoshinari
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Biochem Biophys Res Commun 334:1254-9. 2005
    ..Our finding implies that the splicing endonuclease for the organism is a multi-subunit complex composed of the two endA gene products...
  83. pmc Extensive frameshift at all AGG and CCC codons in the mitochondrial cytochrome c oxidase subunit 1 gene of Perkinsus marinus (Alveolata; Dinoflagellata)
    Isao Masuda
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 0033, Japan
    Nucleic Acids Res 38:6186-94. 2010
    ..To our knowledge, this is the first evidence of translational frameshifts in protist mitochondria and, by far, is the most extensive case in mitochondria...
  84. doi request reprint An anticancer agent, pyrvinium pamoate inhibits the NADH-fumarate reductase system--a unique mitochondrial energy metabolism in tumour microenvironments
    Eriko Tomitsuka
    Cancer Physiology Project, Research Center for Innovative Oncology, National Cancer Center Hospital East, 6 5 1 Kashiwanoha, Kashiwa, Chiba 277 8577, Japan
    J Biochem 152:171-83. 2012
    ..These results indicate that the NADH-FR system may be important for maintaining mitochondrial energy production in tumour microenvironments and suggest its potential use as a novel therapeutic target...
  85. ncbi request reprint Characterization of the dihydroorotate dehydrogenase as a soluble fumarate reductase in Trypanosoma cruzi
    Eizo Takashima
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, Japan
    Mol Biochem Parasitol 122:189-200. 2002
    ..These results clearly indicated that the cytosolic MV-FRD is attributable to T. cruzi DHOD. The DHOD may play an important role in succinate/fumarate metabolism as well as de novo pyrimidine biosynthesis in T. cruzi...
  86. doi request reprint Fasting-induced hypothermia and reduced energy production in mice lacking acetyl-CoA synthetase 2
    Iori Sakakibara
    Laboratory for Systems Biology and Medicine, Research Center for Advanced Science and Technology, University of Tokyo, Tokyo, Japan
    Cell Metab 9:191-202. 2009
    ..Our findings demonstrate that activation of acetate by AceCS2 plays a pivotal role in thermogenesis, especially under low-glucose or ketogenic conditions, and is crucially required for survival...
  87. doi request reprint IL-10 plays a crucial role for the protection of experimental cerebral malaria by co-infection with non-lethal malaria parasites
    Mamoru Niikura
    Institute of Laboratory Animals, Graduate School of Medicine, Kyorin University, Tokyo 181 8611, Japan
    Int J Parasitol 40:101-8. 2010
    ..Co-infection with Pb XAT and Pb ANKA is a useful model for understanding how ECM is suppressed...
  88. ncbi request reprint Coinfection with nonlethal murine malaria parasites suppresses pathogenesis caused by Plasmodium berghei NK65
    Mamoru Niikura
    Institute of Laboratory Animals, Graduate School of Medicine, Faculty of Medicine, Kyorin University, Tokyo, Japan
    J Immunol 180:6877-84. 2008
    ..Moreover, the suppression of liver injury and body weight loss by coinfection was reduced in IL-10(-/-) mice, suggesting that IL-10 plays a role for a reduction of severity by coinfection with nonlethal malaria parasites...
  89. ncbi request reprint Quinones in long-lived clk-1 mutants of Caenorhabditis elegans
    Hiroko Miyadera
    Department of Biomedical Chemistry, Graduate School of Medicine, The University of Tokyo, 7 3 1 Hongo, Bunkyo ku, 113 0033, Tokyo, Japan
    FEBS Lett 512:33-7. 2002
    ..Here we elucidate the possible mechanisms of life span extension in clk-1 mutants, with particular emphasis on the electrochemical property of DMQ. Recent findings on the biochemical function of CLK-1 are also discussed...