Ryoji Nagai

Summary

Affiliation: University of Tokyo
Country: Japan

Publications

  1. doi request reprint Antibody-based detection of advanced glycation end-products: promises vs. limitations
    Ryoji Nagai
    Laboratory of Food and Regulation Biology, Graduate School of Agriculture, Tokai University, Kawayou, Minamiaso, Aso gun, Kumamoto, 869 1404, Japan
    Glycoconj J 33:545-52. 2016
  2. doi request reprint Salacia chinensis L. extract ameliorates abnormal glucose metabolism and improves the bone strength and accumulation of AGEs in type 1 diabetic rats
    Jun Ichi Shirakawa
    Laboratory of Food and Regulation Biology, Graduate School of Agriculture, Tokai University, Kawayou, Minamiaso, Aso gun, Kumamoto 869 1404, Japan
    Food Funct 7:2508-15. 2016
  3. doi request reprint Eucommia ulmoides extracts prevent the formation of advanced glycation end products
    Hikari Sugawa
    Laboratory of Food and Regulation Biology, Department of Bioscience, School of Agriculture, Tokai University, Japan
    Food Funct 7:2566-73. 2016
  4. doi request reprint Inhibition of AGEs formation by natural products
    Ryoji Nagai
    Laboratory of Food and Regulation Biology Department of Bioscience, School of Agriculture, Tokai University, Kawayou Minamiaso Aso gun, Kumamoto, 869 1404, Japan
    Amino Acids 46:261-6. 2014
  5. ncbi request reprint Comparison of pharmacokinetics between highly and mildly modified AGE proteins in mice
    Ryoji Nagai
    Department of Medical Biochemistry, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto 860 8556, Japan
    Ann N Y Acad Sci 1126:325-7. 2008
  6. ncbi request reprint Usefulness of antibodies for evaluating the biological significance of AGEs
    Ryoji Nagai
    Department of Medical Biochemistry, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto 860 8556, Japan
    Ann N Y Acad Sci 1126:38-41. 2008
  7. ncbi request reprint Investigation of pathways of advanced glycation end-products accumulation in macrophages
    Ryoji Nagai
    Department of Medical Biochemistry, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan
    Mol Nutr Food Res 51:462-7. 2007
  8. doi request reprint Immunochemical detection of Nepsilon-(carboxyethyl)lysine using a specific antibody
    Ryoji Nagai
    Department of Medical Biochemistry, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan
    J Immunol Methods 332:112-20. 2008
  9. pmc Citric acid inhibits development of cataracts, proteinuria and ketosis in streptozotocin (type 1) diabetic rats
    Ryoji Nagai
    Department of Food and Nutrition, Laboratory of Biochemistry and Nutritional Science, Japan Women s University, Bunkyo ku, Tokyo 112 8681, Japan
    Biochem Biophys Res Commun 393:118-22. 2010
  10. ncbi request reprint The ligand activity of AGE-proteins to scavenger receptors is dependent on their rate of modification by AGEs
    Ryoji Nagai
    Department of Medical Biochemistry, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, Honjo, 1 1 1, Kumamoto 860 8556, Japan
    Biochim Biophys Acta 1772:1192-8. 2007

Detail Information

Publications70

  1. doi request reprint Antibody-based detection of advanced glycation end-products: promises vs. limitations
    Ryoji Nagai
    Laboratory of Food and Regulation Biology, Graduate School of Agriculture, Tokai University, Kawayou, Minamiaso, Aso gun, Kumamoto, 869 1404, Japan
    Glycoconj J 33:545-52. 2016
    ..This review discusses not only the usability of anti-AGE antibodies to evaluate AGEs and disease pathology and screen AGE inhibitors, but also describes their usage. ..
  2. doi request reprint Salacia chinensis L. extract ameliorates abnormal glucose metabolism and improves the bone strength and accumulation of AGEs in type 1 diabetic rats
    Jun Ichi Shirakawa
    Laboratory of Food and Regulation Biology, Graduate School of Agriculture, Tokai University, Kawayou, Minamiaso, Aso gun, Kumamoto 869 1404, Japan
    Food Funct 7:2508-15. 2016
    ..This study provides evidence to support that SCE ameliorates diabetes-induced abnormalities by improving the uptake of glucose by various organs. ..
  3. doi request reprint Eucommia ulmoides extracts prevent the formation of advanced glycation end products
    Hikari Sugawa
    Laboratory of Food and Regulation Biology, Department of Bioscience, School of Agriculture, Tokai University, Japan
    Food Funct 7:2566-73. 2016
    ..As a result, isoquercetin showed the strongest inhibitory effect of all the tested ELE components. These results indicate that the oral intake of ELE may inhibit the formation of AGEs, thereby ameliorating age-related diseases. ..
  4. doi request reprint Inhibition of AGEs formation by natural products
    Ryoji Nagai
    Laboratory of Food and Regulation Biology Department of Bioscience, School of Agriculture, Tokai University, Kawayou Minamiaso Aso gun, Kumamoto, 869 1404, Japan
    Amino Acids 46:261-6. 2014
    ..Therefore, AGEs inhibitors that can be obtained from daily meals are preferred to prescribed drugs. In this article, we describe a strategy for developing new AGEs inhibitors from natural products. ..
  5. ncbi request reprint Comparison of pharmacokinetics between highly and mildly modified AGE proteins in mice
    Ryoji Nagai
    Department of Medical Biochemistry, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto 860 8556, Japan
    Ann N Y Acad Sci 1126:325-7. 2008
    ..Taken together, our results indicate that the ligand activity of AGE-BSA to scavenger receptors and those pharmacokinetic properties depend on their rate of modification by AGEs...
  6. ncbi request reprint Usefulness of antibodies for evaluating the biological significance of AGEs
    Ryoji Nagai
    Department of Medical Biochemistry, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto 860 8556, Japan
    Ann N Y Acad Sci 1126:38-41. 2008
    ..Taken together, these results demonstrate that a specific antibody is a powerful tool for analyzing novel biomarkers, formation pathways, and the efficacy of AGE inhibitors...
  7. ncbi request reprint Investigation of pathways of advanced glycation end-products accumulation in macrophages
    Ryoji Nagai
    Department of Medical Biochemistry, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan
    Mol Nutr Food Res 51:462-7. 2007
    ..Taken together, our results indicate that AGE detected inside foam cells in atherosclerotic lesions are generated intracellularly rather than representing endocytic uptake of extracellular AGE-proteins by scavenger receptors...
  8. doi request reprint Immunochemical detection of Nepsilon-(carboxyethyl)lysine using a specific antibody
    Ryoji Nagai
    Department of Medical Biochemistry, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan
    J Immunol Methods 332:112-20. 2008
    ..These results demonstrate that a specific antibody against CEL can be a powerful tool for detecting CEL both in vitro and in vivo...
  9. pmc Citric acid inhibits development of cataracts, proteinuria and ketosis in streptozotocin (type 1) diabetic rats
    Ryoji Nagai
    Department of Food and Nutrition, Laboratory of Biochemistry and Nutritional Science, Japan Women s University, Bunkyo ku, Tokyo 112 8681, Japan
    Biochem Biophys Res Commun 393:118-22. 2010
    ..These results demonstrate that the oral administration of citric acid ameliorates ketosis and protects against the development of diabetic complications in an animal model of type 1 diabetes...
  10. ncbi request reprint The ligand activity of AGE-proteins to scavenger receptors is dependent on their rate of modification by AGEs
    Ryoji Nagai
    Department of Medical Biochemistry, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, Honjo, 1 1 1, Kumamoto 860 8556, Japan
    Biochim Biophys Acta 1772:1192-8. 2007
    ....
  11. doi request reprint Natural compounds containing a catechol group enhance the formation of Nε-(carboxymethyl)lysine of the Maillard reaction
    Yukio Fujiwara
    Department of Cell Pathology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto 860 8556, Japan
    Free Radic Biol Med 50:883-91. 2011
    ....
  12. ncbi request reprint Hypochlorous acid generates N epsilon-(carboxymethyl)lysine from Amadori products
    Katsumi Mera
    Department of Medical Biochemistry, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan
    Free Radic Res 41:713-8. 2007
    ..These data demonstrated that HOCl-mediated CML formation from Amadori products plays a role in CML formation and tissue damage at sites of inflammation...
  13. ncbi request reprint Glycolaldehyde-modified bovine serum albumin downregulates leptin expression in mouse adipocytes via a CD36-mediated pathway
    Yuka Unno
    Department of Medical Biochemistry, Kumamoto University Graduate School of Medical and Pharmaceutical Sciences, Honjo 1 1 1, Kumamoto 860 8556, Japan
    Ann N Y Acad Sci 1043:696-701. 2005
    ....
  14. doi request reprint Tomatidine, a tomato sapogenol, ameliorates hyperlipidemia and atherosclerosis in apoE-deficient mice by inhibiting acyl-CoA:cholesterol acyl-transferase (ACAT)
    Yukio Fujiwara
    Department of Cell Pathology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan
    J Agric Food Chem 60:2472-9. 2012
    ..The study provides the first evidence that tomatidine significantly suppresses the activity of ACAT and leads to reduction of atherogenesis...
  15. doi request reprint Glutaraldehyde is an effective cross-linker for production of antibodies against advanced glycation end-products
    Katsumi Mera
    Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan
    J Immunol Methods 334:82-90. 2008
    ..These studies demonstrate the efficacy of glutaraldehyde as a cross-linker for the production of antibodies against small molecules...
  16. doi request reprint Astragalosides isolated from the root of astragalus radix inhibit the formation of advanced glycation end products
    Keita Motomura
    Department of Natural Medicine, Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto 862 0973, Japan
    J Agric Food Chem 57:7666-72. 2009
    ..These data suggest that AR and astragalosides may be a potentially useful strategy for the prevention of clinical diabetic complications by inhibiting AGEs...
  17. ncbi request reprint Pathological roles of advanced glycation end product receptors SR-A and CD36
    Seikoh Horiuchi
    Department of Medical Biochemistry, Kumamoto University Graduate School of Medical and Pharmaceutical Sciences, Honjo 1 1 1, Kumamoto 860 8556, Japan
    Ann N Y Acad Sci 1043:671-5. 2005
    ....
  18. ncbi request reprint Immunological detection of N omega-(Carboxymethyl)arginine by a specific antibody
    Katsumi Mera
    Department of Pharmaceutics, Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto 862 0973, Japan
    Ann N Y Acad Sci 1126:155-7. 2008
    ..This suggests that the accumulation of CMA in tissue proteins may contribute to the pathophysiologies associated with aging and age-related diseases...
  19. doi request reprint Triterpenoids isolated from Zizyphus jujuba inhibit foam cell formation in macrophages
    Yukio Fujiwara
    Department of Cell Pathology, Graduate School of Medical Sciences, Kumamoto University, Kumamoto, Japan
    J Agric Food Chem 59:4544-52. 2011
    ..These data suggest that triterpenoids in Zizyphus jujuba , the plant source of ZF and ZS, may therefore be useful for the prevention of atherosclerosis...
  20. doi request reprint Effect of reactive-aldehydes on the modification and dysfunction of human serum albumin
    Katsumi Mera
    Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan
    J Pharm Sci 99:1614-25. 2010
    ..These results indicated that changes in the physicochemical properties and the formation of AGE structures are highly dependent on the aldehydes...
  21. doi request reprint An autoantibody against N(ε)-(carboxyethyl)lysine (CEL): possible involvement in the removal of CEL-modified proteins by macrophages
    Katsumi Mera
    Department of Biopharmaceutics, Graduate School of Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan
    Biochem Biophys Res Commun 407:420-5. 2011
    ..These results demonstrate that the autoantibody against CEL in plasma may play a role in the macrophage uptake of CEL-modified proteins...
  22. ncbi request reprint Advanced glycation end products-modified proteins and oxidized LDL mediate down-regulation of leptin in mouse adipocytes via CD36
    Yuka Unno
    Department of Medical Biochemistry, Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto, Japan
    Biochem Biophys Res Commun 325:151-6. 2004
    ....
  23. ncbi request reprint Association of advanced glycation end products with A549 cells, a human pulmonary epithelial cell line, is mediated by a receptor distinct from the scavenger receptor family and RAGE
    Nahoko Nakano
    Departments of Medical Biochemistry, Psychiatry, and Cell Pathology, Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University, Honjo 1 1 1, Kumamoto 860 8556
    J Biochem 139:821-9. 2006
    ..Our results indicate that A549 cells possess at least two binding sites, one specific for EN-RAGE and the other specific for AGE-BSA. The latter receptor on A549 cells is distinct from the scavenger receptor family and RAGE...
  24. ncbi request reprint Nepsilon-(Carboxymethyl)lysine and 3-DG-imidazolone are major AGE structures in protein modification by 3-deoxyglucosone
    Tadashi Jono
    Department of Medical Biochemistry, Graduate School of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto, 860 0811, Japan
    J Biochem 136:351-8. 2004
    ..Our results provide the first evidence that CML and 3-DG-imidazolone are major AGE structures in 3-DG-modified proteins, and that 3-DG-imidazolone provides a better marker for protein modification by 3-DG than pyrraline...
  25. ncbi request reprint Peroxynitrite induces formation of N( epsilon )-(carboxymethyl) lysine by the cleavage of Amadori product and generation of glucosone and glyoxal from glucose: novel pathways for protein modification by peroxynitrite
    Ryoji Nagai
    Department of Biochemistry, Kumamoto University School of Medicine, Kumamoto, Japan
    Diabetes 51:2833-9. 2002
    ..Our results provide the first evidence that ONOO(-) can induce protein modification by oxidative cleavage of the Amadori product and also by generation of reactive alpha-oxoaldehydes from glucose...
  26. ncbi request reprint Some natural compounds enhance N epsilon-(carboxymethyl)lysine formation
    Yukio Fujiwara
    Department of Medical Biochemistry, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, Kumamoto 860 8556, Japan
    Ann N Y Acad Sci 1126:152-4. 2008
    ....
  27. pmc Soft-shelled turtle eggs inhibit the formation of AGEs in the serum and skin of diabetic rats
    Mikihiro Yamanaka
    Laboratory of Food and Regulation Biology, Graduate School of Agriculture, Tokai University, Kawayou, Minamiaso, Aso gun, Kumamoto 869 1404, Japan Healthcare Business Development Department I, Medical and Healthcare Business Development Unit, Business Solution Company, SHARP Corporation, 2613 1 Ichinomoto cho, Tenri, Nara 632 8567, Japan
    J Clin Biochem Nutr 58:130-4. 2016
    ..This report provides the first evidence that the oral administration of STE reduces the formation of AGEs, suggesting that one of the health effects of STE may be the inhibition of AGEs formation. ..
  28. pmc Non-invasive measurement of skin autofluorescence to evaluate diabetic complications
    Mikihiro Yamanaka
    Laboratory of Food and Regulation Biology, Graduate School of Agriculture, Tokai University, Kawayou, Minamiaso, Aso gun, Kumamoto 869 1404, Japan Healthcare Business Development Department I, Medical and Healthcare Business Development Unit, Business Solution Company, SHARP Corporation, 2613 1 Ichinomoto cho, Tenri shi, Nara 632 8567, Japan
    J Clin Biochem Nutr 58:135-40. 2016
    ....
  29. ncbi request reprint Conventional antibody against Nepsilon-(carboxymethyl)lysine (CML) shows cross-reaction to Nepsilon-(carboxyethyl)lysine (CEL): immunochemical quantification of CML with a specific antibody
    Wakako Koito
    Department of Medical Biochemistry, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, Honjo 1 1 1, Kumamoto 860 8556, Japan
    J Biochem 136:831-7. 2004
    ..These results indicate that CMS-10 can be used to determine the CML contents of modified proteins in a more specific way...
  30. ncbi request reprint Esculeogenin A, a new tomato sapogenol, ameliorates hyperlipidemia and atherosclerosis in ApoE-deficient mice by inhibiting ACAT
    Yukio Fujiwara
    Department of Medical Biochemistry, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, Honjo, 1 1 1, Kumamoto 860 8556, Japan
    Arterioscler Thromb Vasc Biol 27:2400-6. 2007
    ..In the present study, we examined the effects of esculeoside A and esculeogenin A, a new aglycon of esculeoside A, on foam cell formation in vitro and atherogenesis in apoE-deficient mice...
  31. ncbi request reprint Conversion of Amadori products of the Maillard reaction to N(epsilon)-(carboxymethyl)lysine by short-term heating: possible detection of artifacts by immunohistochemistry
    Cristina Miki Hayashi
    Department of Biochemistry, Kumamoto University School of Medicine, Kumamoto, Japan
    Lab Invest 82:795-808. 2002
    ..CML generated by heating, directly from Amadori products or via these aldehydes, might serve as an artifact upon immunohistochemistry...
  32. doi request reprint Two new prenylflavonoids from Epimedii Herba and their inhibitory effects on advanced glycation end-products
    Keisuke Nakashima
    Faculty of Pharmaceutical Sciences, Sojo University, 4 22 1 Ikeda, Nishi ku, Kumamoto, 860 0082, Japan
    J Nat Med 70:290-5. 2016
    ..These data suggest that epimedokoreanin B could prevent clinical complications of diabetes by inhibiting AGEs. ..
  33. ncbi request reprint Immunohistochemical detection of advanced glycation end products in human bladder with specific monoclonal antibody
    Kenshi Matsumoto
    Department of Urology, Kumamoto University Graduate School of Medicine, Kumamoto, Japan
    Int J Urol 16:402-5; discussion 405-6. 2009
    ..To investigate the accumulation of advanced glycation end products (AGE) in human bladder...
  34. doi request reprint Glyoxalase I overexpression ameliorates renal ischemia-reperfusion injury in rats
    Takanori Kumagai
    Div of Nephrology and Endocrinology, Univ of Tokyo School of Medicine, 7 3 1 Hongo, Bunkyo ku, Tokyo 113 8655, Japan
    Am J Physiol Renal Physiol 296:F912-21. 2009
    ..In conclusion, glyoxalase I exerts renoprotective effects in renal I/R injury via a reduction in MG accumulation in tubular cells...
  35. doi request reprint Immunohistochemical distribution of advanced glycation end products (AGEs) in human osteoarthritic cartilage
    Jun Hirose
    Department of Orthopaedic Surgery, Kumamoto University Hospital, Honjo, Japan
    Acta Histochem 113:613-8. 2011
    ..The coincidental up-regulation of GA-pyridine and RAGE suggests that GA-pyridine is the most significant AGE for cartilage degeneration via the RAGE pathway...
  36. doi request reprint The pentosidine concentration in human blood specimens is affected by heating
    Masako Nakano
    Laboratory of Biochemistry and Nutritional Science, Department of Food and Nutrition, Japan Women s University, 2 8 1 Mejirodai Bunkyo ku, Tokyo, 112 8681, Japan
    Amino Acids 44:1451-6. 2013
    ..This indicates that the level of serum pentosidine will be measured more accurately by ELISA if hydrochloric acid is added during the heating process...
  37. doi request reprint Expression of two isozymes of acyl-coenzyme A: cholesterol acyltransferase-1 and -2 in clear cell type renal cell carcinoma
    Kenshi Matsumoto
    Department of Urology, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, Honjo, Kumamoto, Japan
    Int J Urol 15:166-70. 2008
    ..The present study investigated the expression of acyl-coenzyme A: cholesterol acyltransferase-1 (ACAT-1) and -2 in clear cell type renal cell carcinoma (RCC)...
  38. doi request reprint Angptl 4 deficiency improves lipid metabolism, suppresses foam cell formation and protects against atherosclerosis
    Hironori Adachi
    Department of Metabolic Medicine, Faculty of Medical and Pharmaceutical Sciences, Kumamoto University, 1 1 1 Honjo, Kumamoto 860 8556, Japan
    Biochem Biophys Res Commun 379:806-11. 2009
    ..Thus, genetic knockout of Angptl 4 protects ApoE(-/-) mice against development and progression of atherosclerosis and strongly suppresses the ability of the macrophages to become foam cells in vitro...
  39. doi request reprint Changes in esculeoside A content in different regions of the tomato fruit during maturation and heat processing
    Akiko Katsumata
    Department of Food and Nutrition, Laboratory of Biochemistry and Nutritional Science, Japan Women s University, Tokyo, Japan
    J Agric Food Chem 59:4104-10. 2011
    ..These results demonstrated that the esculeoside A content differs in the various types of tomatoes, during maturation, and during the heating process used for preservation...
  40. pmc Chelation: a fundamental mechanism of action of AGE inhibitors, AGE breakers, and other inhibitors of diabetes complications
    Ryoji Nagai
    Department of Food and Nutrition, Japan Women s University, Tokyo, Japan
    Diabetes 61:549-59. 2012
    ..We conclude that chronic, low-dose chelation therapy deserves serious consideration as a clinical tool for prevention and treatment of diabetes complications...
  41. ncbi request reprint CD36, serves as a receptor for advanced glycation endproducts (AGE)
    Nobutaka Ohgami
    Department of Biofunctional Chemistry, Faculty of Pharmaceutical Sciences, Kumamoto University, 5 1 Ohe Honmachi, 862 0973, Kumamoto, Japan
    J Diabetes Complications 16:56-9. 2002
    ....
  42. ncbi request reprint Chemical modification of muscle protein in diabetes
    Nadja Alt
    Institute of Food Chemistry, Technical University of Dresden, Germany
    Arch Biochem Biophys 425:200-6. 2004
    ..These data indicate that muscle protein is partially protected against the increase in both glycation and AGE/ALE formation in diabetes...
  43. ncbi request reprint Lectin-like oxidized low density lipoprotein receptor-1 (LOX-1) serves as an endothelial receptor for advanced glycation end products (AGE)
    Tadashi Jono
    Department of Biochemistry, Kumamoto University School of Medicine, Honjo 2 2 1, Kumamoto 860 0811, Japan
    FEBS Lett 511:170-4. 2002
    ..Cultured bovine aortic endothelial cells also showed the specific binding for AGE-BSA, which was suppressed by 67% by the anti-BLOX-1 antibody. Thus, LOX-1 is identified as a novel endothelial receptor for AGE...
  44. ncbi request reprint Creatine plays a direct role as a protein modifier in the formation of a novel advanced glycation end product
    Kiminori Miyazaki
    Department of Biochemistry, Kumamoto University School of Medicine, Honjo, Kumamoto 860 0811, Japan
    J Biochem 132:543-50. 2002
    ..This study indicates that creatine plays a direct role as a protein modifier in C-pentosidine formation, although the clinical significance of C-pentosidine is still unknown...
  45. ncbi request reprint Identification in human atherosclerotic lesions of GA-pyridine, a novel structure derived from glycolaldehyde-modified proteins
    Ryoji Nagai
    Department of Biochemistry and Second Department of Pathology, Kumamoto University School of Medicine, Japan
    J Biol Chem 277:48905-12. 2002
    ..These results suggest that myeloperoxidase-mediated protein modification via GA may contribute to atherogenesis...
  46. ncbi request reprint Accumulation of imidazolone, pentosidine and N(epsilon)-(carboxymethyl)lysine in hippocampal CA4 pyramidal neurons of aged human brain
    Tadashi Jono
    Department of Biochemistry, Kumamoto University School of Medicine, Japan
    Pathol Int 52:563-71. 2002
    ..These findings taken together support the notion that the accumulation of AGE structures in the CA4 region might be closely related to the aging process in neurons...
  47. ncbi request reprint Expression and function of receptors for advanced glycation end products in bovine corneal endothelial cells
    Yuichi Kaji
    Department of Ophthalmology, University of Tokyo Graduate School of Medicine, Tokyo, Japan
    Invest Ophthalmol Vis Sci 44:521-8. 2003
    ....
  48. ncbi request reprint Identification of N epsilon-(carboxyethyl)lysine, one of the methylglyoxal-derived AGE structures, in glucose-modified protein: mechanism for protein modification by reactive aldehydes
    Ryoji Nagai
    Department of Biochemistry, Kumamoto University School of Medicine, Honjo 2 2 1, Japan
    J Chromatogr B Analyt Technol Biomed Life Sci 788:75-84. 2003
    ..These results indicate that methylglyoxal is responsible for CEL formation on protein in vitro...
  49. ncbi request reprint Role of megalin in endocytosis of advanced glycation end products: implications for a novel protein binding to both megalin and advanced glycation end products
    Akihiko Saito
    Division of Clinical Nephrology and Rheumatology, Niigata University Graduate School of Medical and Dental Sciences, 1 757 Asahimachi dori, Niigata 951 8510, Japan
    J Am Soc Nephrol 14:1123-31. 2003
    ..In conclusion, megalin functions for endocytosis of AGE via an indirect mechanism. L2 cells express novel AGE-binding proteins, one of which may interact with megalin...
  50. ncbi request reprint Lack of recognition of Nepsilon-(carboxymethyl)lysine by the mouse liver reticulo-endothelial system: implications for pathophysiology
    Dmitri N Svistounov
    Department of Experimental Pathology, Medical Faculty, University of Tromsoe, Norway
    Biochem Biophys Res Commun 309:786-91. 2003
    ..Our results suggest that CML does not engender AGE-BSA clearance. Macromolecules substituted with CML only may escape elimination and cause pathological effects...
  51. ncbi request reprint Increased production of urea hydrogen peroxide from Maillard reaction and a UHP-Fenton pathway related to glycoxidation damage in chronic renal failure
    Akira Moh
    Department of Pathology, Medical School, Yale University, 310 Cedar Street, New Haven, CT, USA
    J Am Soc Nephrol 15:1077-85. 2004
    ..Increased UHP in chronic renal failure enhances the formation of pentosidine and carboxymethyllysine via Fenton reaction (UHP-Fenton pathway)...
  52. ncbi request reprint N(epsilon)-(carboxymethyl)lysine proliferated CD34(+) cells from rat choroidal explant in culture
    Shinjiro Kobayashi
    Department of Pharmacology, Faculty of Pharmaceutical Sciences, Hokuriku University, 3 Ho Kanagawa Machi, Kanazawa 920 1181, Japan
    Biol Pharm Bull 27:1382-7. 2004
    ..The CML-HSA-induced formation of immature blood vessel may be implicated in various choroidal diseases such as age-related macular degeneration...
  53. ncbi request reprint Overproduction of N(epsilon)-(carboxymethyl)lysine-induced neovascularization in cultured choroidal explant of streptozotocin-diabetic rat
    Shinjiro Kobayashi
    Department of Pharmacology, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanagawa machi, Kanazawa, Japan
    Biol Pharm Bull 27:1565-71. 2004
    ..TNFalpha and VEGF are likely to play a predominant role for the CML-induced choroidal neovascularization...
  54. ncbi request reprint S-(2-Succinyl)cysteine: a novel chemical modification of tissue proteins by a Krebs cycle intermediate
    Nathan L Alderson
    Department of Chemistry and Biochemistry, University of South Carolina, USA
    Arch Biochem Biophys 450:1-8. 2006
    ..These observations identify fumarate as an endogenous electrophile and suggest a role for fumarate in regulation of metabolism...
  55. ncbi request reprint Protective effect of heat-processed American ginseng against diabetic renal damage in rats
    Hyun Young Kim
    Institute of Natural Medicine, University of Toyama, 2630 Sugitani, Toyama 930 0194, Japan
    J Agric Food Chem 55:8491-7. 2007
    ..These findings indicate that H-AG may have beneficial effect on pathological conditions associated with diabetic nephropathy...
  56. ncbi request reprint Overproduction of N(epsilon)-(carboxymethyl)lysine-induced neovascularization in cultured choroidal explant of aged rat
    Shinjiro Kobayashi
    Department of Clinical Pharmacology, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa, Japan
    Biol Pharm Bull 30:133-8. 2007
    ..The CML adduct releases TNF alpha which might induce the production and release of VEGF for the abnormal choroidal neovascularization in the patients of age-related macular degeneration...
  57. ncbi request reprint Renal accumulation of pentosidine in non-diabetic proteinuria-induced renal damage in rats
    Femke Waanders
    University Medical Center Groningen, Department of Nephrology, PO Box 30 001, 9700 RB Groningen, The Netherlands
    Nephrol Dial Transplant 20:2060-70. 2005
    ..Secondly, we investigated whether renal AGE accumulation was due to intrarenal effects of local protein trafficking...
  58. doi request reprint Advanced oxidation protein products activate vascular endothelial cells via a RAGE-mediated signaling pathway
    Zhi Jian Guo
    Division of Nephrology, Nanfang Hospital, Guangzhou, China
    Antioxid Redox Signal 10:1699-712. 2008
    ..These data suggested that AOPPs might be new ligands of endothelial RAGE. AOPPs-HSA activates vascular ECs via RAGE-mediated signals...
  59. ncbi request reprint Advanced oxidation protein products accelerate atherosclerosis through promoting oxidative stress and inflammation
    Shang Xi Liu
    Division of Nephrology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, PR China
    Arterioscler Thromb Vasc Biol 26:1156-62. 2006
    ..This study was conducted to test the hypothesis that AOPPs play a causal role in atherosclerosis...
  60. ncbi request reprint Modification of elastin by pentosidine is associated with the calcification of aortic media in patients with end-stage renal disease
    Noriyuki Sakata
    Department of Pathology, School of Medicine, Fukuoka University, Fukuoka, Japan
    Nephrol Dial Transplant 18:1601-9. 2003
    ..The aim of this study was to clarify the role of glycoxidative modification of elastin in the calcification of aortic media in this group of patients...
  61. ncbi request reprint Effect of glucose concentration on formation of AGEs in erythrocytes in vitro
    Ryoji Nagai
    Department of Chemistry and Biochemistry, Graduate Science Research Center, University of South Carolina, Columbia, SC 29208, USA
    Ann N Y Acad Sci 1043:146-50. 2005
    ..The erythrocyte system should be useful for cellular screening of the efficacy of inhibitors of AGE/ALE formation...
  62. ncbi request reprint Vascular change of hippocampal capillary is associated with vascular change of retinal capillary in aging
    Nobuyoshi Hagino
    Laboratory of Kampo Medicine and Neuroendocrinology, Department of Cellular and Structural Biology, The University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Dr, San Antonio, TX 78229 3900, USA
    Brain Res Bull 62:537-47. 2004
    ..In man during senescence, one might also speculate that vascular change of eye capillaries would become the early market for diagnosis of dementia in AD...
  63. ncbi request reprint Protective effect of sun ginseng against diabetic renal damage
    Ki Sung Kang
    Institute of Natural Medicine, University of Toyama, Japan
    Biol Pharm Bull 29:1678-84. 2006
    ..These findings indicate that oxidative stress is increased in the diabetic rat kidney and that SG can prevent renal damage associated with diabetes by attenuating the oxidative stress...
  64. ncbi request reprint Advanced glycation end products (AGE) and the receptor for AGE are present in gastrointestinal tract of familial amyloidotic polyneuropathy patients but do not induce NF-kappaB activation
    Noriko Matsunaga
    Gastroenterology and Hepatology Section, Department of Medicine, Umea University Hospital, 901 85 Umea, Sweden
    Acta Neuropathol 104:441-7. 2002
    ..We conclude that RAGE-AGE or RAGE-TTR interaction might play important roles for gastrointestinal dysfunction and amyloid toxicity, although not through NF-kappaB activation and apoptosis...
  65. ncbi request reprint Renoprotective effects of the AGE-inhibitor pyridoxamine in experimental chronic allograft nephropathy in rats
    Femke Waanders
    University Medical Centre Groningen, Department of Nephrology, Room 40 4 45, P O Box 30 001, 9700 RB Groningen, The Netherlands
    Nephrol Dial Transplant 23:518-24. 2008
    ..The AGE formation inhibitor pyridoxamine (PM) is renoprotective in DN and in normoglycaemic obese Zucker rats. In chronic allograft nephropathy (CAN), renal AGE accumulation occurs as well...
  66. ncbi request reprint Identification of a serum component that regulates cyclooxygenase-2 gene expression in cooperation with 4-hydroxy-2-nonenal
    Masaya Kanayama
    Graduate School of Bioagricultural Sciences, Nagoya University, Nagoya 464 8601, Japan
    J Biol Chem 282:24166-74. 2007
    ..These findings represent a demonstration of a link between lipoprotein modification and activation of the inflammatory potential of macrophages...
  67. ncbi request reprint Succination of protein thiols during adipocyte maturation: a biomarker of mitochondrial stress
    Ryoji Nagai
    Department of Chemistry and Biochemistry, University of South Carolina, Columbia, SC 29208, USA
    J Biol Chem 282:34219-28. 2007
    ..We propose that the increase in fumarate and 2SC is the result of mitochondrial stress in the adipocyte during adipogenesis and that 2SC may be a useful biomarker of mitochondrial stress in obesity, insulin resistance, and diabetes...
  68. ncbi request reprint Effect of AGEs on human disc herniation: intervertebral disc hernia is also effected by AGEs
    Michiyo Tsuru
    Department of Orthopaedic Surgery, Kurume University School of Medicine, Kurume 830 0011, Japan
    Kurume Med J 49:7-13. 2002
    ..Further, AGEs were found to be already exposed during histogenesis, suggesting a relation to apoptosis. In lumbar disc herniation and aging, glucose-derived AGEs cross-link proteins and cause vascular tissue damage...
  69. ncbi request reprint Selective formation of certain advanced glycation end products in spinal cord astrocytes of humans and mice with superoxide dismutase-1 mutation
    Noriyuki Shibata
    Department of Pathology, Tokyo Women s Medical University, Kawada cho 8 1, Shinjuku ku, Tokyo 162 8666, Japan
    Acta Neuropathol 104:171-8. 2002
    ..Our results indicate that protein glycation, but not lipid peroxidation, is enhanced in ALS patients with an SOD1 mutation and mutant SOD1 transgenic mice, in which certain AGEs are selectively formed in the spinal cord astrocytes...
  70. ncbi request reprint Mesangial accumulation of GA-pyridine, a novel glycolaldehyde-derived AGE, in human renal disease
    Wendela L Greven
    Department of Pathology and Laboratory Medicine, and Department of Nephrology, University Medical Center Groningen, Groningen, The Netherlands
    Kidney Int 68:595-602. 2005
    ..As atherosclerosis and progressive glomerulosclerosis share many similarities, we hypothesized that GA-pyridine accumulates in renal diseases, especially those with prominent mesangial involvement...