Laszlo Kohidai

Summary

Affiliation: Chemotaxis Research Group, Dept. Genetics, Cell- & Immunobiology, Semmelweis University
Location: Budapest, Hungary
URL: www.chemotaxis.usn.hu
Summary:
Head of the Chemotaxis Research Group
Investigations of
- chemotaxis
- cell adhgesion
- phylogeny of signal molecules
- hormonal imprinting - epigenetics
- drug targeting
- impedimetry based cell physiological assays
- endogenous bioactive substances in protozoa

Most frequently applied model cells:
- tumor cell lines (leukemia, melanoma, adeno cc.)
- Tetrahymena pyriformis

Publications

  1. ncbi request reprint Chemotactic-range-fitting of amino acids and its correlations to physicochemical parameters in Tetrahymena pyriformis--evolutionary consequences
    L Kohidai
    Department of Genetics, Cell and Immunobiology, Semmelweis University, Nagyvárad tér 4 Budapest, 1089 Hungary
    Cell Mol Biol (Noisy-le-grand) 49:OL487-95. 2003
  2. ncbi request reprint Chemotactic selection of Tetrahymena pyriformis GL induced with histamine, di-iodotyrosine or insulin
    L Kohidai
    Department of Genetics, Cell and Immunobiology, Semmelweis University of Medicine, Budapest, Hungary
    Comp Biochem Physiol C Toxicol Pharmacol 126:1-9. 2000
  3. ncbi request reprint A short-run new analytical ultracentrifugal micromethod for determining low-density lipoprotein sub-fractions using Schlieren refractometry
    Z Bozoky
    National Research Institute for Radiobiology and Radiohygiene, Budapest, Hungary
    Eur Biophys J 29:621-7. 2001
  4. ncbi request reprint Chemotactic selection with insulin, di-iodotyrosine and histamine alters the phagocytotic responsiveness of Tetrahymena
    N Schiess
    Department of Genetics, Cell and Immunobiology, Semmelweis University, H 1445, P O Box 370, Budapest, Hungary
    Comp Biochem Physiol C Toxicol Pharmacol 128:521-30. 2001
  5. ncbi request reprint Direct chemotactic effect of bradykinin and related peptides-significance of amino- and carboxyterminal character of oligopeptides in chemotaxis of tetrahymena pyriformis
    László Kohidai
    Department of Genetics, Cell and Immunobiology, Semmelweis University, Budapest, H 1089, Hungary
    Cell Biol Int 26:55-62. 2002
  6. ncbi request reprint Synthesis of oligopeptides with the sequence SXWS and their chemotactic effects on a ciliated protozoan Tetrahymena pyriformis
    Eszter Illyes
    Department of Organic Chemistry, Eotvos Lorand University, Budapest, Hungary
    J Pept Sci 8:13-22. 2002
  7. ncbi request reprint Effect of vasoactive peptides on Tetrahymena. chemotactic properties of endothelins (ET-1, ET-2, ET-3, fragment 11-21 of ET-1 and big endothelin-1): a short-term inducible signalling mechanism of chemotaxis
    L Kohidai
    Department of Genetics, Cell and Immunobiology, Semmelweis University, Budapest, Hungary
    Cell Biol Int 25:1173-7. 2001
  8. ncbi request reprint Melatonin in the unicellular Tetrahymena pyriformis: effects of different lighting conditions
    L Kohidai
    Department of Genetics, Cell and Immunobiology, Semmelweis University, Budapest, Hungary
    Cell Biochem Funct 20:269-72. 2002
  9. ncbi request reprint Effects of steroid hormones on five functional parameters of Tetrahymena: evolutionary conclusions
    László Kohidai
    Department of Genetics, Cell and Immunobiology, Semmelweis University, Budapest, Hungary
    Cell Biochem Funct 21:19-26. 2003
  10. ncbi request reprint Chemotactic activity of oligopeptides containing an EWS motif on Tetrahymena pyriformis: the effect of amidation of the C-terminal residue
    László Kohidai
    Department of Genetics, Cell and Immunobiology, Semmelweis University, Budapest, Nagyvarad ter 4, H 1089, Hungary
    Cell Biochem Funct 21:113-20. 2003

Collaborators

Detail Information

Publications62

  1. ncbi request reprint Chemotactic-range-fitting of amino acids and its correlations to physicochemical parameters in Tetrahymena pyriformis--evolutionary consequences
    L Kohidai
    Department of Genetics, Cell and Immunobiology, Semmelweis University, Nagyvárad tér 4 Budapest, 1089 Hungary
    Cell Mol Biol (Noisy-le-grand) 49:OL487-95. 2003
    ....
  2. ncbi request reprint Chemotactic selection of Tetrahymena pyriformis GL induced with histamine, di-iodotyrosine or insulin
    L Kohidai
    Department of Genetics, Cell and Immunobiology, Semmelweis University of Medicine, Budapest, Hungary
    Comp Biochem Physiol C Toxicol Pharmacol 126:1-9. 2000
    ..T2 data suggest that the long lasting responsiveness is not general, but might be subpopulation specific...
  3. ncbi request reprint A short-run new analytical ultracentrifugal micromethod for determining low-density lipoprotein sub-fractions using Schlieren refractometry
    Z Bozoky
    National Research Institute for Radiobiology and Radiohygiene, Budapest, Hungary
    Eur Biophys J 29:621-7. 2001
    ....
  4. ncbi request reprint Chemotactic selection with insulin, di-iodotyrosine and histamine alters the phagocytotic responsiveness of Tetrahymena
    N Schiess
    Department of Genetics, Cell and Immunobiology, Semmelweis University, H 1445, P O Box 370, Budapest, Hungary
    Comp Biochem Physiol C Toxicol Pharmacol 128:521-30. 2001
    ..These experiments call attention to the evolutionary role of selection in the later developing receptor-hormone relationship...
  5. ncbi request reprint Direct chemotactic effect of bradykinin and related peptides-significance of amino- and carboxyterminal character of oligopeptides in chemotaxis of tetrahymena pyriformis
    László Kohidai
    Department of Genetics, Cell and Immunobiology, Semmelweis University, Budapest, H 1089, Hungary
    Cell Biol Int 26:55-62. 2002
    ..Study of chemotactic selection with BK derivatives supports our previous findings that only phylogenetically selected ligands or their close derivatives are able to induce long-term selection with chemotaxis...
  6. ncbi request reprint Synthesis of oligopeptides with the sequence SXWS and their chemotactic effects on a ciliated protozoan Tetrahymena pyriformis
    Eszter Illyes
    Department of Organic Chemistry, Eotvos Lorand University, Budapest, Hungary
    J Pept Sci 8:13-22. 2002
    ..Hca-SKWS, however, bound remarkably to the cell surface. The binding activity of the Hca-peptides was less pronounced than that of the naOx-peptides, indicating the importance of the fluorophores applied...
  7. ncbi request reprint Effect of vasoactive peptides on Tetrahymena. chemotactic properties of endothelins (ET-1, ET-2, ET-3, fragment 11-21 of ET-1 and big endothelin-1): a short-term inducible signalling mechanism of chemotaxis
    L Kohidai
    Department of Genetics, Cell and Immunobiology, Semmelweis University, Budapest, Hungary
    Cell Biol Int 25:1173-7. 2001
    ..Chemotactic selection with endothelins underlines the possibility that there may be separate mechanisms responsible for the short-term chemotactic responses and the long-lasting effects of chemotactic selection...
  8. ncbi request reprint Melatonin in the unicellular Tetrahymena pyriformis: effects of different lighting conditions
    L Kohidai
    Department of Genetics, Cell and Immunobiology, Semmelweis University, Budapest, Hungary
    Cell Biochem Funct 20:269-72. 2002
    ..In contrast to this, storage of melatonin was observed, which was more expressed in dark conditions. Prolonged light exposure suppressed melatonin production and secretion alike, however it did not block it completely...
  9. ncbi request reprint Effects of steroid hormones on five functional parameters of Tetrahymena: evolutionary conclusions
    László Kohidai
    Department of Genetics, Cell and Immunobiology, Semmelweis University, Budapest, Hungary
    Cell Biochem Funct 21:19-26. 2003
    ..The results call attention to the fine molecular recognition capacity of Tetrahymena and to the possible rapid effects of steroid hormones at membrane receptors at a very low evolutionary eukaryotic level...
  10. ncbi request reprint Chemotactic activity of oligopeptides containing an EWS motif on Tetrahymena pyriformis: the effect of amidation of the C-terminal residue
    László Kohidai
    Department of Genetics, Cell and Immunobiology, Semmelweis University, Budapest, Nagyvarad ter 4, H 1089, Hungary
    Cell Biochem Funct 21:113-20. 2003
    ..The high discriminative ability and good chemotactic responsiveness of Tetrahymena support the theory that a chemotactic signalling mechanism working in higher levels of phylogeny is a well conserved and inducible one even in protozoa...
  11. ncbi request reprint Characterization of chemotactic ability of peptides containing N-formyl-methionyl residues in Tetrahymena fMLP as a targeting ligand
    László Kohidai
    Department of Genetics, Cell and Immunobiology, Semmelweis University of Medicine, Nagyvarad ter 4 POB 370, 1445, Budapest, Hungary
    Cell Biol Int 27:695-700. 2003
    ..The results call attention to the possible presence of f-Met receptors at a unicellular level and to the evolutionary conservation of chemotaxis-activating processes...
  12. ncbi request reprint Induction of melatonin synthesis in Tetrahymena pyriformis by hormonal imprinting--a unicellular "factory" of the indoleamine
    L Kohidai
    Department of Genetics, Cell and Immunobiology, Semmelweis University of Medicine, Budapest, Hungary
    Cell Mol Biol (Noisy-le-grand) 49:521-4. 2003
    ..The experiments call attention to the light-sensitivity of imprinting-provoked melatonin production in Tetrahymena and to the possibility of using this property for important physiological functions in higher grades of phylogeny...
  13. ncbi request reprint Synthesis, conformation, and immunoreactivity of new carrier molecules based on repeated tuftsin-like sequence
    Gabor Mezo
    Research Group of Peptide Chemistry, Hungarian Academy of Sciences, Eotvos L University, Budapest, Hungary
    Biopolymers 73:645-56. 2004
    ..Data suggest that these new oligotuftsin derivatives can be considered as promising carriers for synthetic vaccine...
  14. ncbi request reprint Cell-physiological effects of elastin derived (VGVAPG)n oligomers in a unicellular model system
    László Kohidai
    Department of Genetics, Cell and Immunobiology, Semmelweis University Budapest, H 1089 Budapest, Hungary
    J Pept Sci 10:427-38. 2004
    ..The characteristic cell-physiological effects of VGVAPG oligomers signal that molecules of the extracellular matrix can induce identical responses even in lower levels of phylogeny, e.g. in the Ciliates...
  15. ncbi request reprint Synthesis of oligotuftsin-based branched oligopeptide conjugates for chemotactic drug targeting
    Gabor Mezo
    Research Group of Peptide Chemistry, Hungarian Academy of Sciences, Eotvos L University, 1518 Budapest, Hungary
    J Pept Sci 12:328-36. 2006
    ..The spacer with N-terminal methotrexate was coupled to the chloroacetylated chemotactic carrier molecule by thioether bond formation. The chemotactic activity and cytotoxity of Mtx conjugates were also studied...
  16. ncbi request reprint Effect of tuftsin and oligotuftsins on chemotaxis and chemotactic selection in Tetrahymena pyriformis
    O Lang
    Department of Genetics, Cell and Immunobiology, Semmelweis University, Budapest, Nagyvárad tér 4 H 1089, Hungary
    Cell Biol Int 30:603-9. 2006
    ..The chemotactic selection data indicated that tuftsin-induced chemotaxis results from fairly short-term signalling in Tetrahymena...
  17. doi request reprint In vitro cytotoxicity, chemotactic effect, and cellular uptake of branched polypeptides with poly[L-lys] backbone by J774 murine macrophage cell line
    Rita Szabó
    Research Group of Peptide Chemistry at Eötvös L University, Hungarian Academy of Sciences, Budapest 112, POB 32, H 1518, Hungary
    Bioconjug Chem 19:1078-86. 2008
    ....
  18. doi request reprint Design, synthesis, and in vitro activity of novel drug delivery systems containing tuftsin derivatives and methotrexate
    Katalin B Bai
    Department of Organic Chemistry, Eotvos L University, Budapest 112, Hungary
    Bioconjug Chem 19:2260-9. 2008
    ..The results suggest that our tuftsin-based drug delivery systems might be potential candidates for targeting cancer chemotherapy...
  19. doi request reprint Daunomycin-polypeptide conjugates with antitumor activity
    Rita Szabó
    Research Group of Peptide Chemistry, Hungarian Academy of Sciences, Eotvos L University, Budapest 112, Hungary
    Biochim Biophys Acta 1798:2209-16. 2010
    ..We found that attachment of polypeptide and cell penetrating peptide to the bioactive agent, depending on the cell line, could significantly improve the antitumor activity of the drug...
  20. doi request reprint Chemotactic effect of odorants and tastants on the ciliate Tetrahymena pyriformis
    Júlia Láng
    Department of Genetics, Cell and Immunobiology, Semmelweis University
    J Recept Signal Transduct Res 31:423-33. 2011
    ..No significant activation of PLC or PI3K could be observed following stimulation with attractant odorants which implies that some other pathways may be involved, hence further investigation is needed...
  21. doi request reprint Basic cell physiological activities (cell adhesion, chemotaxis and proliferation) induced by selegiline and its derivatives in Mono Mac 6 human monocytes
    Eszter Lajkó
    Department of Genetics Cell and Immunobiology, Semmelweis University, Nagyvarad ter 4, Budapest 1089, Hungary
    J Neural Transm (Vienna) 119:545-56. 2012
    ..In summary, the adhesion inducer activity, chemorepellent and advantageous cytotoxic effects of selegiline and some derivatives indicate that these drug molecules might have inhibitory effects in metastasis formation in primary tumors...
  22. doi request reprint Chemotaxis induced by SXWS tetrapeptides in Tetrahymena--overlapping chemotactic effects of SXWS sequences and their identical amino acids
    Orsolya Láng
    Department of Genetics, Cell and Immunobiology, Semmelweis University, Nagyvarad ter 4, Budapest, 1089, Hungary
    J Mol Recognit 25:24-31. 2012
    ....
  23. doi request reprint GnRH-III based multifunctional drug delivery systems containing daunorubicin and methotrexate
    Ulrike Leurs
    Laboratory of Analytical Chemistry and Biopolymer Structure Analysis, Department of Chemistry, University of Konstanz, 78457 Konstanz, Germany
    Eur J Med Chem 52:173-83. 2012
    ....
  24. doi request reprint Effects of the aquatic contaminant human pharmaceuticals and their mixtures on the proliferation and migratory responses of the bioindicator freshwater ciliate Tetrahymena
    Júlia Láng
    Department of Genetics, Cell and Immunobiology, Semmelweis University, H 1445 Budapest, Hungary
    Chemosphere 89:592-601. 2012
    ..In summary authors suggest Tetrahymena as a sensible model of testing aquatic contaminants as well as underline the significance using more specific endpoints to understand the complex mechanisms investigated...
  25. ncbi request reprint Verification of epigenetic inheritance in a unicellular model system: multigenerational effects of hormonal imprinting
    László Kohidai
    Department of Genetics, Cell and Immunobiology, Semmelweis University, Budapest, Hungary
    Cell Biol Int 36:951-9. 2012
    ..The results - together with results obtained earlier in mammals - point to the validity of epigenetic imprinting effects throughout the animal world...
  26. doi request reprint Investigation on chemotactic drug targeting (chemotaxis and adhesion) inducer effect of GnRH-III derivatives in Tetrahymena and human leukemia cell line
    Eszter Lajkó
    Department of Genetics Cell and Immunobiology, Semmelweis University, Nagyvarad ter 4, H 1089, Budapest, Hungary
    J Pept Sci 19:46-58. 2013
    ....
  27. ncbi request reprint The effect of varying illumination on imprinting of Tetrahymena by insulin
    L Kohidai
    Department of Biology, Semmelweis University Medical School, Budapest, Hungary
    Acta Microbiol Hung 34:179-85. 1987
    ..It is suggested that the dark-induced structural transformation of the membrane, manifesting among others in a changed hormone binding, may be caused by alterations in haem synthesis due to varying illumination...
  28. ncbi request reprint Effects of hormones on chemotaxis in Tetrahymena: investigations on receptor memory
    L Kohidai
    Department of Biology, Semmelweis University of Medicine, Budapest, Hungary
    Microbios 77:75-85. 1994
    ..The first encounter with these molecules may modify the chemotactic behaviour of the progeny cells. In a significant number of cases there was a different response after repeated encounters of cells and hormones...
  29. pmc Targeted tumor therapy by Rubia tinctorum L.: analytical characterization of hydroxyanthraquinones and investigation of their selective cytotoxic, adhesion and migration modulator effects on melanoma cell lines (A2058 and HT168-M1)
    Eszter Lajkó
    Department of Genetics, Cell and Immunobiology, Semmelweis University, Nagyv rad t r 4, Budapest, 1089 Hungary
    Cancer Cell Int 15:119. 2015
    ....
  30. doi request reprint Effect of vasoactive peptides in Tetrahymena: chemotactic activities of adrenomedullin, proadrenomedullin N-terminal 20 peptide (PAMP) and calcitonin gene-related peptide (CGRP)
    László Kohidai
    Department of Genetics, Cell and Immunobiology, Semmelweis University, Nagyvarad ter 4, Budapest, 1089, Hungary
    Mol Cell Biochem 411:271-80. 2016
    ..The results draw attention to the characteristic effects of vasoactive peptides at this low level of phylogeny. ..