Antos Shakhbazau

Summary

Publications

  1. doi request reprint Autologous mesenchymal stromal cells as a therapeutic in ALS and epilepsy patients: Treatment modalities and ex vivo neural differentiation
    Antos Shakhbazau
    Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada Electronic address
    Cytotherapy 18:1245-55. 2016
  2. doi request reprint Fluorescent Phosphorus Dendrimer as a Spectral Nanosensor for Macrophage Polarization and Fate Tracking in Spinal Cord Injury
    Antos Shakhbazau
    Hotchkiss Brain Institute and Cumming School of Medicine, University of Calgary, HRIC 1AA02, 3280 Hospital Drive, NW, T2N4Z6, Calgary, Canada
    Macromol Biosci 15:1523-34. 2015
  3. doi request reprint Inter-microcarrier transfer and phenotypic stability of stem cell-derived Schwann cells in stirred suspension bioreactor culture
    Antos Shakhbazau
    Hotchkiss Brain Institute, Cumming School of Medicine, University of Calgary, Calgary, Canada
    Biotechnol Bioeng 113:393-402. 2016
  4. doi request reprint Aligned collagen-GAG matrix as a 3D substrate for Schwann cell migration and dendrimer-based gene delivery
    Antos Shakhbazau
    Department of Clinical Neuroscience, Faculty of Medicine, University of Calgary, HMRB 109 3330 Hospital Drive NW, Calgary, AB, T2N4N1, Canada
    J Mater Sci Mater Med 25:1979-89. 2014
  5. pmc Fourth generation phosphorus-containing dendrimers: prospective drug and gene delivery carrier
    D Shcharbin
    Institute of Biophysics and Cell Engineering of Natl Acad Sci, Minsk, Belarus
    Pharmaceutics 3:458-73. 2011
  6. doi request reprint Doxycycline-regulated GDNF expression promotes axonal regeneration and functional recovery in transected peripheral nerve
    Antos Shakhbazau
    Department of Clinical Neuroscience, Faculty of Medicine, University of Calgary, Calgary, Canada Hotchkiss Brain Institute, University of Calgary, Calgary, Canada Electronic address
    J Control Release 172:841-51. 2013
  7. doi request reprint Evidence for a systemic regulation of neurotrophin synthesis in response to peripheral nerve injury
    Antos Shakhbazau
    Department of Clinical Neuroscience, Faculty of Medicine, University of Calgary, Calgary, Canada
    J Neurochem 122:501-11. 2012
  8. ncbi request reprint Non-viral engineering of skin precursor-derived Schwann cells for enhanced NT-3 production in adherent and microcarrier culture
    A Shakhbazau
    Department of Clinical Neuroscience, Faculty of Medicine, University of Calgary, Calgary, Canada
    Curr Med Chem 19:5572-9. 2012
  9. doi request reprint Early regenerative effects of NGF-transduced Schwann cells in peripheral nerve repair
    Antos Shakhbazau
    Department of Clinical Neuroscience, Faculty of Medicine, University of Calgary, Calgary, Canada
    Mol Cell Neurosci 50:103-12. 2012
  10. doi request reprint Dendrimer-driven neurotrophin expression differs in temporal patterns between rodent and human stem cells
    Antos Shakhbazau
    Department of Clinical Neurosciences, Faculty of Medicine, University of Calgary, Calgary, Canada
    Mol Pharm 9:1521-8. 2012

Collaborators

Detail Information

Publications23

  1. doi request reprint Autologous mesenchymal stromal cells as a therapeutic in ALS and epilepsy patients: Treatment modalities and ex vivo neural differentiation
    Antos Shakhbazau
    Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada Electronic address
    Cytotherapy 18:1245-55. 2016
    ..We review the lessons from animal models and clinical trials, and consider encouraging newly published clinical data on therapeutic applications of neurally induced MSCs. ..
  2. doi request reprint Fluorescent Phosphorus Dendrimer as a Spectral Nanosensor for Macrophage Polarization and Fate Tracking in Spinal Cord Injury
    Antos Shakhbazau
    Hotchkiss Brain Institute and Cumming School of Medicine, University of Calgary, HRIC 1AA02, 3280 Hospital Drive, NW, T2N4Z6, Calgary, Canada
    Macromol Biosci 15:1523-34. 2015
    ..Potential use of a single dendrimer compound as a drug/siRNA carrier and phenotype-specific cell tracer offers new avenues for enhanced cell therapies combined with monitoring of cell fate and function in spinal cord injury. ..
  3. doi request reprint Inter-microcarrier transfer and phenotypic stability of stem cell-derived Schwann cells in stirred suspension bioreactor culture
    Antos Shakhbazau
    Hotchkiss Brain Institute, Cumming School of Medicine, University of Calgary, Calgary, Canada
    Biotechnol Bioeng 113:393-402. 2016
    ..Scaled-up microcarrier-based production of SKP-SCs in suspension bioreactors appears feasible for timely generation of sufficient cell numbers for nerve repair strategies...
  4. doi request reprint Aligned collagen-GAG matrix as a 3D substrate for Schwann cell migration and dendrimer-based gene delivery
    Antos Shakhbazau
    Department of Clinical Neuroscience, Faculty of Medicine, University of Calgary, HMRB 109 3330 Hospital Drive NW, Calgary, AB, T2N4N1, Canada
    J Mater Sci Mater Med 25:1979-89. 2014
    ..We have also demonstrated that SCs cultured within collagen-GAG matrix are compatible with non-viral dendrimer-based gene delivery, that may allow conditioning of matrix-embedded cells for future gene therapy applications. ..
  5. pmc Fourth generation phosphorus-containing dendrimers: prospective drug and gene delivery carrier
    D Shcharbin
    Institute of Biophysics and Cell Engineering of Natl Acad Sci, Minsk, Belarus
    Pharmaceutics 3:458-73. 2011
    ..Based on the results, the fourth generation cationic phosphorus-containing dendrimers seem to be a good drug and gene delivery carrier candidate. ..
  6. doi request reprint Doxycycline-regulated GDNF expression promotes axonal regeneration and functional recovery in transected peripheral nerve
    Antos Shakhbazau
    Department of Clinical Neuroscience, Faculty of Medicine, University of Calgary, Calgary, Canada Hotchkiss Brain Institute, University of Calgary, Calgary, Canada Electronic address
    J Control Release 172:841-51. 2013
    ....
  7. doi request reprint Evidence for a systemic regulation of neurotrophin synthesis in response to peripheral nerve injury
    Antos Shakhbazau
    Department of Clinical Neuroscience, Faculty of Medicine, University of Calgary, Calgary, Canada
    J Neurochem 122:501-11. 2012
    ..Axonal regeneration was studied at 2 weeks time-point using pan-neuronal marker NF-200 and found to directly correlate with NGF levels in the regenerating nerve...
  8. ncbi request reprint Non-viral engineering of skin precursor-derived Schwann cells for enhanced NT-3 production in adherent and microcarrier culture
    A Shakhbazau
    Department of Clinical Neuroscience, Faculty of Medicine, University of Calgary, Calgary, Canada
    Curr Med Chem 19:5572-9. 2012
    ..Further work to develop bioprocesses for expansion of SKP-SCs on microcarriers in bioreactors is still needed...
  9. doi request reprint Early regenerative effects of NGF-transduced Schwann cells in peripheral nerve repair
    Antos Shakhbazau
    Department of Clinical Neuroscience, Faculty of Medicine, University of Calgary, Calgary, Canada
    Mol Cell Neurosci 50:103-12. 2012
    ....
  10. doi request reprint Dendrimer-driven neurotrophin expression differs in temporal patterns between rodent and human stem cells
    Antos Shakhbazau
    Department of Clinical Neurosciences, Faculty of Medicine, University of Calgary, Calgary, Canada
    Mol Pharm 9:1521-8. 2012
    ..Nonviral neurotrophin expression is a biologically safe and accessible alternative to increase the therapeutic potential of autologous adult stem cells and stem cell-derived functional differentiated cells...
  11. ncbi request reprint Neurons and stromal stem cells as targets for polycation-mediated transfection
    A V Shakhbazau
    Department of Clinical Neurobiology, University of Calgary, Canada
    Bull Exp Biol Med 151:126-9. 2011
    ..Expression of neurotrophic factor BDNF in MSC transfected with polyamidoamine dendrimers was also by more than 10 times higher...
  12. doi request reprint Use of polyamidoamine dendrimers to engineer BDNF-producing human mesenchymal stem cells
    Antos Shakhbazau
    Institute of Genetics and Cytology, National Academy of Sciences of Belarus, Akademicheskaya, 27, 220072, Minsk, Belarus
    Mol Biol Rep 37:2003-8. 2010
    ..Non-virally induced neurotrophin expression may be a safe and easy method for adapting autologous stem cells for therapeutic treatment of diseases and neural system injuries...
  13. ncbi request reprint Plasticity of human mesenchymal stem cell phenotype and expression profile under neurogenic conditions
    A V Shakhbazau
    Institute of Cytology and Genetics, National Academy of Sciences of Belarus, Minsk, Belarus
    Bull Exp Biol Med 147:513-6. 2009
    ..This indicates high plasticity of the phenotype and expression profile of neuronal markers in MSC cultured under neurogenic conditions or possibility of dedifferentiation of MSC reaching the stage of neuronal precursors/early neurons...
  14. ncbi request reprint Neurogenic induction of human mesenchymal stem cells in fibrin 3D matrix
    A V Shakhbazau
    Institute of Genetics and Cytology, National Academy of Sciences of Belarus, Minsk, Belarus
    Bull Exp Biol Med 150:547-50. 2011
    ..Optimal combination of 3D matrix and neurogenic factors of the medium can provide new insight into neurogenic potential of mesenchymal stem cells, which can be used for the therapy of neural traumas and neurodegenerative diseases...
  15. ncbi request reprint Viral vectors for stable transduction of human mesenchymal stem cells: systems based on adeno-associated viruses and lentiviruses
    A V Shakhbazau
    Institute of Genetics and Cytology, National Academy of Sciences of Belarus, Minsk, Belarussia
    Bull Exp Biol Med 146:531-3. 2008
    ..We compared the efficiency of transduction with lentivectors based on HIV-1 and adeno-associated viruses serotype 2 and stability of transgene expression in human mesenchymal bone marrow stem cells...
  16. doi request reprint Skin derived precursor Schwann cells improve behavioral recovery for acute and delayed nerve repair
    Helene T Khuong
    Hotchkiss Brain Institute and Department of Clinical Neurosciences, Faculty of Medicine, University of Calgary, 3330 Hospital Drive NW, Calgary, Alberta T2N4N1, Canada Service de Neurochirurgie, Département des Sciences Neurologiques, CHU de Québec Hôpital de l Enfant Jésus, Centre de Recherche du CHU de Québec, Canada Division de Neurochirurgie, Departement de Chirurgie, Universite Laval, 1401, 18e rue, Quebec, Quebec G1J 1Z4, Canada
    Exp Neurol 254:168-79. 2014
    ..In conclusion, SKP-SC therapy improves behavioral recovery after acute, chronic and nerve graft repair beyond the current standard of microsurgical nerve repair. ..
  17. doi request reprint Non-virally modified human mesenchymal stem cells produce ciliary neurotrophic factor in biodegradable fibrin-based 3D scaffolds
    Antos Shakhbazau
    Department of Clinical Neuroscience, Faculty of Medicine, University of Calgary, Calgary, Alberta, Canada
    J Pharm Sci 101:1546-54. 2012
    ..Nonviral modification of autologous adult stem cells with use of dendrimers is a novel tool perspective in terms of biosafety and technological availability...
  18. doi request reprint Transfection efficiencies of PAMAM dendrimers correlate inversely with their hydrophobicity
    Antos Shakhbazau
    Institute of Genetics and Cytology of Natl Acad Sci Bel, Minsk, Belarus
    Int J Pharm 383:228-35. 2010
    ..Lipofectamine 2000 was a more effective carrier (18.5%) than PAMAM G4 dendrimer (1.2%)...
  19. doi request reprint Phosphorus-containing nanoparticles: biomedical patents review
    Dzmitry Shcharbin
    Institute of Biophysics and Cell Engineering of NASB, Minsk, Belarus
    Expert Opin Ther Pat 25:539-48. 2015
    ..The beginning of the nano-era started with the appearance of artificial nanosized supramolecular systems called nanomaterials and nanoparticles (NPs)...
  20. doi request reprint Sensory recovery after cell therapy in peripheral nerve repair: effects of naïve and skin precursor-derived Schwann cells
    Antos Shakhbazau
    Department of Clinical Neuroscience, Faculty of Medicine
    J Neurosurg 121:423-31. 2014
    ..This report describes long-term functional regenerative effects of cell therapy in the rat injury model with a focus on sensory recovery...
  21. doi request reprint Poly(amidoamine) dendrimer complexes as a platform for gene delivery
    Dzmitry Shcharbin
    Institute of Biophysics and Cell Engineering of NASB, Minsk, Belarus
    Expert Opin Drug Deliv 10:1687-98. 2013
    ..It is based on several viral and non-viral systems for nucleic acid delivery. The number of clinical trials based on application of non-viral drug and gene delivery systems is rapidly increasing...
  22. doi request reprint Demyelination induces transport of ribosome-containing vesicles from glia to axons: evidence from animal models and MS patient brains
    Antos Shakhbazau
    Hotchkiss Brain Institute and Cumming School of Medicine, University of Calgary, 3330 Hospital Drive NW, Calgary, AB, T2N 4N1, Canada
    Mol Biol Rep 43:495-507. 2016
    ....
  23. pmc The impact of motor axon misdirection and attrition on behavioral deficit following experimental nerve injuries
    Jacob Daniel de Villiers Alant
    Department of Clinical Neurosciences, Hotchkiss Brain Institute, University of Calgary, Calgary, Alberta, Canada
    PLoS ONE 8:e82546. 2013
    ..It also demonstrates that clinically relevant neuroma-in-continuity injuries can now also be subjected to experimental investigation. ..