Zhiqiang Gao

Summary

Affiliation: National University of Singapore
Country: Singapore

Publications

  1. doi request reprint Enzyme-catalysed deposition of ultrathin silver shells on gold nanorods: a universal and highly efficient signal amplification strategy for translating immunoassay into a litmus-type test
    Xinjian Yang
    Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Republic of Singapore
    Chem Commun (Camb) 51:6928-31. 2015
  2. doi request reprint A simple and highly sensitive fluorescence assay for microRNAs
    Wei Shen
    Department of Chemistry, National University of Singapore, Singapore 117543
    Analyst 140:1932-8. 2015
  3. doi request reprint Carbon quantum dots and their applications
    Shi Ying Lim
    Department of Chemistry, National University of Singapore, Singapore 117543
    Chem Soc Rev 44:362-81. 2015
  4. doi request reprint Gold nanoparticle-based exonuclease III signal amplification for highly sensitive colorimetric detection of folate receptor
    Xinjian Yang
    Department of Chemistry, National University of Singapore, Singapore 117543
    Nanoscale 6:3055-8. 2014
  5. doi request reprint An ultrasensitive homogeneous chemiluminescent assay for microRNAs
    Huimin Deng
    Department of Chemistry, National University of Singapore, Singapore 117543
    Chem Commun (Camb) 49:9401-3. 2013
  6. doi request reprint A label-free biosensor for electrochemical detection of femtomolar microRNAs
    Zhiqiang Gao
    Department of Chemistry, National University of Singapore, Singapore 117543
    Anal Chem 85:1624-30. 2013
  7. doi request reprint Detection of single-nucleotide polymorphisms based on the formation of an electron-transfer impeding layer on an electrode surface
    Zhiqiang Gao
    Department of Chemistry, National University of Singapore, 117543, Singapore
    Chem Commun (Camb) 49:370-2. 2013
  8. doi request reprint A highly sensitive electrochemical assay for microRNA expression profiling
    Zhiqiang Gao
    Department of Chemistry, National University of Singapore, Singapore 117543
    Analyst 137:1674-9. 2012
  9. doi request reprint A label-free microRNA biosensor based on DNAzyme-catalyzed and microRNA-guided formation of a thin insulating polymer film
    Wei Shen
    Department of Chemistry, National University of Singapore, Singapore 117543, Singapore
    Biosens Bioelectron 44:171-6. 2013
  10. doi request reprint An interference-free glucose biosensor based on an anionic redox polymer-mediated enzymatic oxidation of glucose
    Huimin Deng
    Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Republic of Singapore
    Chemphyschem 14:2343-7. 2013

Collaborators

  • Wei Shen
  • Hong Xie
  • Xiaojun Chen
  • Huimin Deng
  • Xinjian Yang
  • Yuqian Ren
  • Shi Ying Lim
  • Natalia C Tansil
  • Si Ying Peng
  • Yuebo Yu
  • Stephanie Pei Xing Yeo
  • Yanfen Peng
  • Guangshun Yi
  • Fang Xie

Detail Information

Publications31

  1. doi request reprint Enzyme-catalysed deposition of ultrathin silver shells on gold nanorods: a universal and highly efficient signal amplification strategy for translating immunoassay into a litmus-type test
    Xinjian Yang
    Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Republic of Singapore
    Chem Commun (Camb) 51:6928-31. 2015
    ....
  2. doi request reprint A simple and highly sensitive fluorescence assay for microRNAs
    Wei Shen
    Department of Chemistry, National University of Singapore, Singapore 117543
    Analyst 140:1932-8. 2015
    ..Successful attempts were made in applying the proposed assay to detect let-7a in total RNA extracted from cultured cells. ..
  3. doi request reprint Carbon quantum dots and their applications
    Shi Ying Lim
    Department of Chemistry, National University of Singapore, Singapore 117543
    Chem Soc Rev 44:362-81. 2015
    ....
  4. doi request reprint Gold nanoparticle-based exonuclease III signal amplification for highly sensitive colorimetric detection of folate receptor
    Xinjian Yang
    Department of Chemistry, National University of Singapore, Singapore 117543
    Nanoscale 6:3055-8. 2014
    ..A detection limit of 50 fM FR was obtained using UV-vis spectrometry and 10 pM FR could be visualized by the naked eye. ..
  5. doi request reprint An ultrasensitive homogeneous chemiluminescent assay for microRNAs
    Huimin Deng
    Department of Chemistry, National University of Singapore, Singapore 117543
    Chem Commun (Camb) 49:9401-3. 2013
    ..By leveraging on the cumulative and selective nature of the DSN amplification process, excellent sensitivity and selectivity are accomplished. ..
  6. doi request reprint A label-free biosensor for electrochemical detection of femtomolar microRNAs
    Zhiqiang Gao
    Department of Chemistry, National University of Singapore, Singapore 117543
    Anal Chem 85:1624-30. 2013
    ..0 fM to 2.0 pM with a detection limit of 2.0 fM. The biosensor is applied to the detection of circulating miRNAs in blood and miRNAs in total RNA extracted from cultured cells...
  7. doi request reprint Detection of single-nucleotide polymorphisms based on the formation of an electron-transfer impeding layer on an electrode surface
    Zhiqiang Gao
    Department of Chemistry, National University of Singapore, 117543, Singapore
    Chem Commun (Camb) 49:370-2. 2013
    ..Excellent selectivity and sensitivity are accomplished through the engagement of a nuclease clean-up step and a cumulative signal generation/amplification process, respectively...
  8. doi request reprint A highly sensitive electrochemical assay for microRNA expression profiling
    Zhiqiang Gao
    Department of Chemistry, National University of Singapore, Singapore 117543
    Analyst 137:1674-9. 2012
    ..A detection limit of 4.0 fM and a linear calibration curve up to 10 pM were obtained under optimal conditions. The assay was applied to profile human let-7 miRNA expressions in cultured cancer cells...
  9. doi request reprint A label-free microRNA biosensor based on DNAzyme-catalyzed and microRNA-guided formation of a thin insulating polymer film
    Wei Shen
    Department of Chemistry, National University of Singapore, Singapore 117543, Singapore
    Biosens Bioelectron 44:171-6. 2013
    ..The accumulative nature of the PDB deposition drastically improves the detectability of the biosensor. A proof-of-concept study is conducted on the detection of miRNAs in total RNA extracted from cultured cells...
  10. doi request reprint An interference-free glucose biosensor based on an anionic redox polymer-mediated enzymatic oxidation of glucose
    Huimin Deng
    Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543, Republic of Singapore
    Chemphyschem 14:2343-7. 2013
    ..The low hydrophobicity of the composite membrane also effectively retards the transport of molecular oxygen within the membrane...
  11. doi request reprint A highly sensitive and selective electrochemical biosensor for direct detection of microRNAs in serum
    Yuqian Ren
    Department of Chemistry, National University of Singapore, Singapore 117543, Singapore
    Anal Chem 85:4784-9. 2013
    ..Attempts are made in direct profiling circulating miRNAs in serum and miRNAs in total RNA extracted from cancer cells. ..
  12. doi request reprint A real-time colorimetric assay for label-free detection of microRNAs down to sub-femtomolar levels
    Wei Shen
    Department of Chemistry, National University of Singapore, Singapore 117543
    Chem Commun (Camb) 49:4959-61. 2013
    ..Excellent sensitivity and selectivity were accomplished through the engagement of the DSN and a cumulative signal amplification process...
  13. ncbi request reprint Synthesis and anticancer properties of a novel bis-intercalator
    Wei Shen
    Department of Chemistry, National University of Singapore, Singapore 117543
    Anticancer Agents Med Chem 13:632-8. 2013
    ..Attractive anticancer activity is observed with the bisintercalator, which provides a new lead in the anticancer drug design strategy...
  14. doi request reprint An electronic sensor array for label-free detection of single-nucleotide polymorphisms
    Wei Shen
    Department of Chemistry, National University of Singapore, Singapore 117543, Singapore
    Biosens Bioelectron 43:165-72. 2013
    ..Attempts are made in applying the sensor array to the detection of SNPs in DNA samples extracted from tissues and cultured cells...
  15. ncbi request reprint Colorimetric detection of single-nucleotide polymorphisms with a real-time PCR-like sensitivity
    Wei Shen
    Department of Chemistry, National University of Singapore, 3 Science Drive 3, 117543, Singapore
    Chem Commun (Camb) 48:10225-7. 2012
    ..One mutant in 2000 copies of a wild-type gene can be detected in 20-100 pg of PCR-amplified genomic DNA samples...
  16. doi request reprint A highly sensitive microRNA biosensor based on hybridized microRNA-guided deposition of polyaniline
    Huimin Deng
    Department of Chemistry, National University of Singapore, Singapore 117543, Republic of Singapore
    Biosens Bioelectron 60:195-200. 2014
    ..Attempts were made in profiling miRNAs in total RNA samples extracted from cancer cells and blood...
  17. doi request reprint A ferrofluid-based homogeneous assay for highly sensitive and selective detection of single-nucleotide polymorphisms
    Wei Shen
    Department of Chemistry, National University of Singapore, Singapore
    Chem Commun (Camb) 49:8114-6. 2013
    ..Excellent sensitivity and selectivity were accomplished through the engagement of the FNPs and a ligase chain reaction. ..
  18. doi request reprint A highly sensitive and selective homogenous assay for profiling microRNA expression
    Huimin Deng
    Department of Chemistry, National University of Singapore, Singapore 117543, Singapore
    Biosens Bioelectron 54:650-5. 2014
    ..The isothermal amplification scheme together with the simple assay protocol makes direct profiling miRNA in real-world samples possible. ..
  19. doi request reprint Gold nanoparticle-enabled real-time ligation chain reaction for ultrasensitive detection of DNA
    Wei Shen
    Department of Chemistry, National University of Singapore, Singapore 117543
    J Am Chem Soc 134:14678-81. 2012
    ..The cumulative nature of the protocol produced a detection limit of 20 aM with a selectivity factor of 10(3)...
  20. ncbi request reprint An ultrasensitive nucleic acid biosensor based on the catalytic oxidation of guanine by a novel redox threading intercalator
    Natalia C Tansil
    Institute of Bioengineering and Nanotechnology, 31 Biopolis Way, Singapore, 138669, Singapore
    Chem Commun (Camb) . 2005
    ..0-5.0 [corrected] microl droplets is described which can be used for detection of cancer marker genes in mRNA extracted from human breast tissues without a RT-PCR step...
  21. doi request reprint MoS2 nanosheets as an effective fluorescence quencher for DNA methyltransferase activity detection
    Huimin Deng
    Department of Chemistry, National University of Singapore, 3 Science Drive 3, Singapore 117543 chmgaoz nus edu sg
    Analyst 140:3210-5. 2015
    ..Based on this assay, a linear range of 0.2-20 U mL(-1) is achieved with high sensitivity and selectivity. Furthermore, the inhibitor screening ability is well demonstrated. ..
  22. doi request reprint Nanoparticulate peroxidase/catalase mimetic and its application
    Huimin Deng
    Department of Chemistry, National University of Singapore, Singapore 117543, Singapore
    Chemistry 18:8906-11. 2012
    ..0 and peroxidation at pH 4.0 in aqueous solutions (see scheme). Their specific activities are better than many natural catalases and peroxidases and far better than bifunctional catalase/peroxidases...
  23. ncbi request reprint Breast cancer susceptibility gene mRNAs quantified by microarrays with electrochemical detection
    Hong Xie
    Institute of Bioengineering and Nanotechnology, Singapore, Republic of Singapore
    Clin Chem 50:1231-3. 2004
  24. doi request reprint Colorimetric detection of single nucleotide polymorphisms in the presence of 10³-fold excess of a wild-type gene
    Huimin Deng
    Department of Chemistry, National University of Singapore, 117543 Singapore, Singapore
    Biosens Bioelectron 68:310-5. 2015
    ..40 fM and a dynamic range from 1.0 to 200 fM. The simple protocol and its high sensitivity and selectivity allowed the proposed assay to be used in the detection of SNPs in genomic DNA samples...
  25. doi request reprint Silver/gold core-shell nanoprism-based plasmonic nanoprobes for highly sensitive and selective detection of hydrogen sulfide
    Xinjian Yang
    Department of Chemistry, National University of Singapore, Singapore 117543 Singapore
    Chemistry 21:988-92. 2015
    ..Moreover, the SPR peak is located in the NIR region that makes these plasmonic nanoprobes more appealing for the detection of hydrogen sulfide in real-world samples and in in vivo applications...
  26. doi request reprint Highly sensitive electrochemical methyltransferase activity assay
    Huimin Deng
    Department of Chemistry, National University of Singapore, Singapore 117543
    Anal Chem 86:2117-23. 2014
    ..SssI MTase ranged from 0 to 120 U/mL with a current sensitivity of 0.046 μA mL U(-1) is obtained. The inhibitor screening ability of the developed MTase activity assay is also demonstrated. ..
  27. doi request reprint Quantum dots and duplex-specific nuclease enabled ultrasensitive detection and serotyping of Dengue viruses in one step in a single tube
    Wei Shen
    Department of Chemistry, National University of Singapore, Singapore 117543, Singapore
    Biosens Bioelectron 65:327-32. 2015
    ..The highly efficient DSN cleavage in conjunction with its excellent mismatch discrimination ability permits serotyping of DVs in one tube with excellent sensitivity and selectivity. ..
  28. doi request reprint Highly sensitive detection of M.SssI DNA methyltransferase activity using a personal glucose meter
    Huimin Deng
    Department of Chemistry, National University of Singapore, Science Drive 3, Singapore, 117543, Singapore
    Anal Bioanal Chem 408:5867-72. 2016
    ..SssI MTase. In addition, inhibition studies with 5'-azacytidine demonstrate the capability of inhibition screening using this method. Graphical abstract Deteciton of M.SssI DNA methyltransferase activity by a personal glucose meter. ..
  29. doi request reprint Bioanalytical applications of isothermal nucleic acid amplification techniques
    Huimin Deng
    Department of Chemistry, National University of Singapore, Singapore 117543
    Anal Chim Acta 853:30-45. 2015
    ..Representative examples illustrating the performance and advantages of each isothermal amplification technique are discussed along with some discussion on the advantages and disadvantages of each technique. ..
  30. doi request reprint A highly sensitive and specific biosensor for ligation- and PCR-free detection of microRNAs
    Zhiqiang Gao
    Department of Chemistry, National University of Singapore, Singapore 117543, Singapore
    Biosens Bioelectron 26:3768-73. 2011
    ..Successful attempts were made in applying the biosensor to the detection of miRNAs in total RNA extracted from cell lines...
  31. doi request reprint A highly sensitive plasmonic DNA assay based on triangular silver nanoprism etching
    Xinjian Yang
    Department of Chemistry, National University of Singapore, Singapore 117543
    ACS Nano 8:4902-7. 2014
    ..0 fM target DNA. Considering the high sensitivity and selectivity of this plasmonic DNA assay, it is expected to be of great interest in clinical diagnostics. ..