D Steverding

Summary

Publications

  1. ncbi request reprint Trypanocidal and cysteine protease inhibitory activity of isopentyl caffeate is not linked in Trypanosoma brucei
    Dietmar Steverding
    Bob Champion Research and Education Building, Norwich Medical School, University of East Anglia, Norwich Research Park, James Watson Road, Norwich, NR4 7UQ, UK
    Parasitol Res 115:4397-4403. 2016
  2. pmc In vitro activity of salinomycin and monensin derivatives against Trypanosoma brucei
    Dietmar Steverding
    Bob Champion Research and Education Building, Norwich Medical School, University of East Anglia, Norwich, UK
    Parasit Vectors 9:409. 2016
  3. pmc Evaluation of marking of peer marking in oral presentation
    Dietmar Steverding
    Norwich Medical School, University of East Anglia, Norwich Research Park, Norwich, UK
    Perspect Med Educ 5:103-7. 2016
  4. doi request reprint Evaluation of trypanocidal activity of combinations of anti-sleeping sickness drugs with cysteine protease inhibitors
    Dietmar Steverding
    Biomedical Research Centre, Norwich Medical School, University of East Anglia, Norwich, United Kingdom Electronic address
    Exp Parasitol 151:28-33. 2015
  5. pmc The history of Chagas disease
    Dietmar Steverding
    Biomedical Research Centre, Norwich Medical School, Norwich Research Park, University of East Anglia, Norwich NR4 7TJ, UK
    Parasit Vectors 7:317. 2014
  6. pmc A new initiative for the development of new diagnostic tests for human African trypanosomiasis
    Dietmar Steverding
    Biomedical Research Centre, School of Medicine, Health Policy and Practice, University of East Anglia, Norwich NR4 7TJ, UK
    Kinetoplastid Biol Dis 5:1. 2006
  7. pmc Visible spectral distribution of shadows explains why blue targets with a high reflectivity at 460 nm are attractive to tsetse flies
    Dietmar Steverding
    Biomedical Research Centre, Norwich Medical School, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK
    Parasit Vectors 6:285. 2013
  8. doi request reprint Trypanosoma brucei transferrin receptor can bind C-lobe and N-lobe fragments of transferrin
    Dietmar Steverding
    Norwich Medical School, Biomedical Research Centre, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK
    Mol Biochem Parasitol 185:99-105. 2012
  9. doi request reprint Trypanosoma brucei: chemical evidence that cathepsin L is essential for survival and a relevant drug target
    Dietmar Steverding
    Biomedical Research Centre, Norwich Medical School, University of East Anglia, Norwich NR4 7TJ, UK
    Int J Parasitol 42:481-8. 2012
  10. doi request reprint In vitro antifungal activity of DNA topoisomerase inhibitors
    Dietmar Steverding
    Norwich Medical School, University of East Anglia, Norwich, UK
    Med Mycol 50:333-6. 2012

Collaborators

Detail Information

Publications49

  1. ncbi request reprint Trypanocidal and cysteine protease inhibitory activity of isopentyl caffeate is not linked in Trypanosoma brucei
    Dietmar Steverding
    Bob Champion Research and Education Building, Norwich Medical School, University of East Anglia, Norwich Research Park, James Watson Road, Norwich, NR4 7UQ, UK
    Parasitol Res 115:4397-4403. 2016
    ..This is supported by a more than 100 times less sensitivity of human HL-60 cells to isopentyl caffeate indicating that the ester has a favourable selectivity profile...
  2. pmc In vitro activity of salinomycin and monensin derivatives against Trypanosoma brucei
    Dietmar Steverding
    Bob Champion Research and Education Building, Norwich Medical School, University of East Anglia, Norwich, UK
    Parasit Vectors 9:409. 2016
    ..In this study, derivatives of the polyether ionophores, salinomycin and monensin were tested for their in vitro activity against bloodstream forms of Trypanosoma brucei and human HL-60 cells...
  3. pmc Evaluation of marking of peer marking in oral presentation
    Dietmar Steverding
    Norwich Medical School, University of East Anglia, Norwich Research Park, Norwich, UK
    Perspect Med Educ 5:103-7. 2016
    ..However, the use of peer marking in summative assessment is not widely adopted because many teachers are concerned about biased marking by students of their peers...
  4. doi request reprint Evaluation of trypanocidal activity of combinations of anti-sleeping sickness drugs with cysteine protease inhibitors
    Dietmar Steverding
    Biomedical Research Centre, Norwich Medical School, University of East Anglia, Norwich, United Kingdom Electronic address
    Exp Parasitol 151:28-33. 2015
    ....
  5. pmc The history of Chagas disease
    Dietmar Steverding
    Biomedical Research Centre, Norwich Medical School, Norwich Research Park, University of East Anglia, Norwich NR4 7TJ, UK
    Parasit Vectors 7:317. 2014
    ..Recently, migration of T. cruzi-infected patients has led to a distribution of Chagas disease from Latin America to non-endemic countries in Europe, North America and western Pacific region. ..
  6. pmc A new initiative for the development of new diagnostic tests for human African trypanosomiasis
    Dietmar Steverding
    Biomedical Research Centre, School of Medicine, Health Policy and Practice, University of East Anglia, Norwich NR4 7TJ, UK
    Kinetoplastid Biol Dis 5:1. 2006
    ..The Foundation for Innovative New Diagnostics and the World Health Organization have announced that they will collaborate to develop and evaluate new diagnostic tests for human African trypanosomiasis...
  7. pmc Visible spectral distribution of shadows explains why blue targets with a high reflectivity at 460 nm are attractive to tsetse flies
    Dietmar Steverding
    Biomedical Research Centre, Norwich Medical School, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK
    Parasit Vectors 6:285. 2013
    ..As the visible spectral distribution of shadows has a maximum at around 460 nm it is suggested that the response to blue is due to the search of tsetse flies for shadows to seek for potential hosts, resting sites or cover...
  8. doi request reprint Trypanosoma brucei transferrin receptor can bind C-lobe and N-lobe fragments of transferrin
    Dietmar Steverding
    Norwich Medical School, Biomedical Research Centre, University of East Anglia, Norwich Research Park, Norwich NR4 7TJ, UK
    Mol Biochem Parasitol 185:99-105. 2012
    ..This difference may be exploited for the development of agents specifically interfering with the binding of Tf to the TbTfR...
  9. doi request reprint Trypanosoma brucei: chemical evidence that cathepsin L is essential for survival and a relevant drug target
    Dietmar Steverding
    Biomedical Research Centre, Norwich Medical School, University of East Anglia, Norwich NR4 7TJ, UK
    Int J Parasitol 42:481-8. 2012
    ..All inhibitors, except the CATB-specific inhibitor, CA-074, blockaded lysosomal hydrolysis prior to death. The results suggest that TbCATL, rather than TbCATB, is essential to the survival of T. brucei and an appropriate drug target...
  10. doi request reprint In vitro antifungal activity of DNA topoisomerase inhibitors
    Dietmar Steverding
    Norwich Medical School, University of East Anglia, Norwich, UK
    Med Mycol 50:333-6. 2012
    ..glabrata and C. neoformans with MIC(50) values in the mid micromolar range. The data of this study indicate that selected DNA topoisomerase inhibitors are a promising class of compounds for the development of new antifungal agents...
  11. doi request reprint Trypanocidal activity of β-lactone-γ-lactam proteasome inhibitors
    Dietmar Steverding
    Biomedical Research Centre, Norwich Medical School, University of East Anglia, Norwich, UK
    Planta Med 78:131-4. 2012
    ..In general, trypanosomes were more susceptible to the compounds than were human HL-60 cells. The data support the potential of β-lactone- γ-lactam proteasome inhibitors for rational anti-trypanosomal drug development...
  12. pmc Ochronosis as an unusual cause of valvular defect: a case report
    Andreas Wilke
    Kardiologische Praxis Papenburg, Papenburg, Germany
    J Med Case Reports 3:9302. 2009
    ..Alkaptonuria (also known as ochronosis) is a genetic disorder characterised by the accumulation of homogentisic acid deposits in connective tissue. In rare cases, ochronosis can cause valvular heart disease...
  13. ncbi request reprint Ubiquitination of plasma membrane ectophosphatase in bloodstream forms of Trypanosoma brucei
    D Steverding
    Abteilung Parasitologie, Hygiene Institut der Ruprecht Karls Universtät, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany
    Parasitol Res 98:157-61. 2006
    ..As ubiquitin modification of plasma membrane proteins serves as an internalization signal, it is suggested that ubiquitinated ectophosphatase is labelled for endocytosis...
  14. ncbi request reprint Novel antitrypanosomal agents
    Dietmar Steverding
    School of Medicine, Health Policy and Practice, University of East Anglia, Norwich NR4 TJ7, UK
    Expert Opin Investig Drugs 14:939-55. 2005
    ..This article summarises the recent progress in identifying novel lead compounds for antitrypanosomal chemotherapy. Particular emphasis is placed on those agents showing promising, selective antitrypanosomal activity...
  15. ncbi request reprint Evaluation of the anti-trypanosomal activity of tyropeptin A
    Dietmar Steverding
    Biomedical Research Centre, School of Medicine, Health Policy and Practice, University of East Anglia, Norwich, UK
    Planta Med 72:761-3. 2006
    ..The results suggest that natural compounds targeting the trypsin-like activity of the proteasome may serve as leads for rational drug development of novel anti-trypanosomal agents...
  16. ncbi request reprint Cysteine proteinase inhibitors as therapy for parasitic diseases: advances in inhibitor design
    Dietmar Steverding
    Biomedical Research Centre, School of Medicine, Health Policy and Practice, University of East Anglia, Norwich NR4 7TJ, UK
    Mini Rev Med Chem 6:1025-32. 2006
    ..This article reviews the biology and physicochemistry of parasite proteinases and the ongoing design of peptidyl and non-peptidyl inhibitors to generate anti-parasitic compounds of greater efficacy with decreased toxicity to the host...
  17. ncbi request reprint On the significance of host antibody response to the Trypanosoma brucei transferrin receptor during chronic infection
    Dietmar Steverding
    School of Medicine, Health Policy and Practice, University of East Anglia, Norwich, UK
    Microbes Infect 8:2777-82. 2006
    ..Altogether, the results suggest that the host antibody response to the TbTfR during chronic infection with T. brucei is too low, if present at all, to prevent sufficient iron uptake by bloodstream forms to promote their growth...
  18. doi request reprint Trypanocidal activity of peptidyl vinyl ester derivatives selective for inhibition of mammalian proteasome trypsin-like activity
    Dietmar Steverding
    Biomedical Research Centre, Norwich Medical School, University of East Anglia, Norwich, United Kingdom
    Exp Parasitol 128:444-7. 2011
    ..This finding shows that the inhibitor sensitivities between mammalian and trypanosome proteasome are distinct. This difference may be exploited for rational anti-trypanosomal drug development...
  19. pmc The history of African trypanosomiasis
    Dietmar Steverding
    Biomedical Research Centre, School of Medicine, Health Policy and Practice, University of East Anglia, Norwich NR4 7TJ, UK
    Parasit Vectors 1:3. 2008
    ..Current history of human African trypanosomiasis has shown that the production of anti-sleeping sickness drugs is not always guaranteed, and therefore, new, better and cheaper drugs are urgently required...
  20. pmc The development of drugs for treatment of sleeping sickness: a historical review
    Dietmar Steverding
    Biomedical Research Centre, School of Medicine, Health Policy and Practice, University of East Anglia, Norwich NR4 7TJ, UK
    Parasit Vectors 3:15. 2010
    ..This review summarises the developmental processes which led to these chemotherapies from the discovery of the first bioactive lead compounds to the identification of the final drugs...
  21. pmc Efficacy of common laboratory disinfectants and heat on killing trypanosomatid parasites
    Xia Wang
    Biomedical Research Centre, School of Medicine, Health Policy and Practice, University of East Anglia, Norwich, NR4 7TJ, UK
    Parasit Vectors 1:35. 2008
    ..The results indicate that the disinfectants, water and temperature treatment (i.e. autoclaving) are suitable laboratory hygiene measures against trypanosomatid parasites...
  22. pmc Trypanocidal activity of the proteasome inhibitor and anti-cancer drug bortezomib
    Dietmar Steverding
    Biomedical Research Centre, School of Medicine, Health Policy and Practice, University of East Anglia, Norwich, NR4 7TJ, UK
    Parasit Vectors 2:29. 2009
    ..The results suggest that bortezomib may be useful as drug for the treatment of human African trypanosomiasis...
  23. doi request reprint The trypanocidal effect of NO-releasing agents is not due to inhibition of the major cysteine proteinase in Trypanosoma brucei
    Dietmar Steverding
    Biomedical Research Centre, School of Medicine, Health Policy and Practice, University of East Anglia, Norwich NR4 7TJ, UK
    Parasitol Res 105:1333-8. 2009
    ..The results show that the trypanocidal activity of NO donors cannot be attributed to the inhibition of the major lysosomal cysteine proteinase in bloodstream forms of T. brucei...
  24. ncbi request reprint Effect of Australian tea tree oil on Gyrodactylus spp. infection of the three-spined stickleback Gasterosteus aculeatus
    Dietmar Steverding
    School of Biological Sciences, University of Bristol, Woodland Road, Bristol BS8 1UG, UK
    Dis Aquat Organ 66:29-32. 2005
    ..In addition, Tween 80 alone exhibited parasiticidal activity against Gyrodactylus spp. These findings show the potential of TTO in combination with Tween 80 as an effective treatment of Gyrodactylus spp. infection of fishes...
  25. ncbi request reprint Trypanocidal activities of trileucine methyl vinyl sulfone proteasome inhibitors
    Dietmar Steverding
    School of Biological Sciences, University of Bristol, Woodland Road, Bristol, BS8 1UG, UK
    Parasitol Res 95:73-6. 2005
    ..These data suggest that inhibitors with strong activity against the trypsin-like activity of the proteasome are the rational choice for future anti-sleeping sickness drug development...
  26. ncbi request reprint Trypanosoma brucei: unexpected azide sensitivity of bloodstream forms
    Dietmar Steverding
    School of Biological Sciences, University of Bristol, Bristol BS8 1UG, UK
    J Parasitol 90:1188-90. 2004
    ..5 mM azide (a concentration at which no cell proliferates), the toxic action of azide cannot be due to inhibition of this enzyme. These results indicate that the general toxicity of azide is different from that of cyanide...
  27. pmc On the role of blue shadows in the visual behaviour of tsetse flies
    Dietmar Steverding
    School of Biological Sciences, University of Bristol, Woodland Road, Bristol BS8 1UG, UK
    Proc Biol Sci 271:S16-7. 2004
    ..In contrast to people's experience that daytime shadows are colourless, actually on a sunny day all shadows are tinted bluish by the scattered blue skylight...
  28. ncbi request reprint The significance of transferrin receptor variation in Trypanosoma brucei
    Dietmar Steverding
    School of Biological Sciences, University of Bristol, Woodland Road, Bristol, UK BS8 1UG
    Trends Parasitol 19:125-7. 2003
    ..This article shows that calculations based on K(d) values argue against the first part of the hypothesis, but might support the second part...
  29. pmc Identification of a developmentally regulated iron superoxide dismutase of Trypanosoma brucei
    M Kabiri
    Abteilung Parasitologie, , Im Neuenheimer Feld 324, D-69120 Heidelberg, Germany
    Biochem J 360:173-7. 2001
    ....
  30. ncbi request reprint Trypanosoma evansi: demonstration of a transferrin receptor derived from expression site-associated genes 6 and 7
    M Kabiri
    Abteilung Parasitologie, , Heidelberg, Germany
    J Parasitol 87:1189-91. 2001
    ..evansi is completely inhibited with anti-T. brucei (ESAG6/7 heterodimer) antibodies. The demonstration of a functional ESAG6/7 transferrin receptor in T. evansi supports further its close relationship to T. brucei...
  31. ncbi request reprint Trypanocidal effect of alpha',beta'-epoxyketones indicates that trypanosomes are particularly sensitive to inhibitors of proteasome trypsin-like activity
    Robert J Glenn
    School of Biological Sciences, University of Bristol, Woodland Road, Bristol BS8 1UG, UK
    Int J Antimicrob Agents 24:286-9. 2004
    ..In conclusion, the data suggests that proteasome inhibitors targeting the trypsin-like activity are the rational choice for future anti-trypanosomal drug development...
  32. ncbi request reprint Bloodstream forms of Trypanosoma brucei require only small amounts of iron for growth
    D Steverding
    Hygiene Institut der Ruprecht Karls Universität, Abteilung Parasitologie, Heidelberg, Germany
    Parasitol Res 84:59-62. 1998
    ..Calculations revealed that accumulation of about 40,000 iron atoms during 1 generation-doubling time was sufficient for parasite multiplication...
  33. ncbi request reprint The transferrin receptor of Trypanosoma brucei
    D Steverding
    Abteilung Parasitologie, Hygiene Institut, Ruprecht Karls Universitat, Im Neuenheimer Feld 324, D 69120 Heidelberg, Germany
    Parasitol Int 48:191-8. 2000
    ..In this review, the structure, biochemical properties and function of the transferrin receptor of T. brucei are summarized and compared to the transferrin receptor of mammalian cells...
  34. doi request reprint In vitro trypanocidal activity of the anti-helminthic drug niclosamide
    Karin Merschjohann
    Department of Parasitology, Ruprecht Karls University, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany
    Exp Parasitol 118:637-40. 2008
    ..b. brucei and T. congolense, respectively. The very low toxicity of niclosamide for mammals makes the compound interesting for drug development for African trypanosomiasis...
  35. ncbi request reprint Improved trypanocidal activities of cathepsin L inhibitors
    Njinkeng Joseph Nkemgu
    Abteilung Parasitologie, Hygiene Institut der Ruprecht Karls Universität, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany
    Int J Antimicrob Agents 22:155-9. 2003
    ..Also, all inhibitors blocked proteinolysis in the lysosome consistent with the inhibition of rhodesain/brucipain. In conclusion, the data support the potential of cathepsin L inhibitors for rational anti-trypanosomal drug development...
  36. ncbi request reprint Growth inhibition of bloodstream forms of Trypanosoma brucei by the iron chelator deferoxamine
    Tanja Breidbach
    Abteilung Parasitologie, Hygiene Institut der Ruprecht Karls Universität, Im Neuenheimer Feld 324, D 69120 Heidelberg, Germany
    Int J Parasitol 32:473-9. 2002
    ..The results have implications for antitrypanosomal drug development based on specific intervention with the parasite's iron metabolism...
  37. doi request reprint Expression and purification of non-glycosylated Trypanosoma brucei transferrin receptor in insect cells
    Alexander Maier
    Department of Parasitology, Ruprecht Karls University, Im Neuenheimer Feld 324, 69120 Heidelberg, Germany
    Exp Parasitol 120:205-7. 2008
    ..The non-glycosylated ESAG6 and ESAG7 were capable of forming a heterodimer and of binding transferrin. This results shows that glycosylation is not necessary for synthesis of a functional T. brucei transferrin receptor...
  38. pmc Antitrypanosomal activities of proteasome inhibitors
    Njinkeng Joseph Nkemngu
    Abteilung Parasitologie, Hygiene Institut Institut für Pharmazeutische Biologie, Ruprecht Karls Universitat, Heidelberg, Germany
    Antimicrob Agents Chemother 46:2038-40. 2002
    ..In general, trypanosomes were more susceptible to the compounds than were human HL-60 cells. The data support the potential of proteasome inhibitors for rational antitrypanosomal drug development...
  39. ncbi request reprint In vitro effect of alkaloids on bloodstream forms of Trypanosoma brucei and T. congolense
    K Merschjohann
    Abteilung Parasitologie, Hygiene-Institut, , Heidelberg, Germany
    Planta Med 67:623-7. 2001
    ..DNA intercalation in combination with protein biosynthesis inhibition, which is the major mode of action of the active alkaloids, could be responsible for the observed trypanocidal and cytotoxic effects...
  40. ncbi request reprint Increased trypanolytic activity in sera of sleeping sickness patients after chemotherapy
    A Fischer
    Abteilung Parasitologie, , Heidelberg, Germany
    Trop Med Int Health 6:1070-4. 2001
    ..These results suggest that trypanolytic activity of sera increases after African sleeping sickness and is directed against trypanosomes expressing metacyclic VSG...
  41. ncbi request reprint Identification and functional characterization of thioredoxin from Trypanosoma brucei brucei
    N Reckenfelderbäumer
    Biochemie Zentrum Heidelberg, Universitat Heidelberg, 69120 Heidelberg, Germany
    J Biol Chem 275:7547-52. 2000
    ..brucei ribonucleotide reductase. The parasite protein is the first classical thioredoxin of the order Kinetoplastida characterized so far...
  42. ncbi request reprint Anti-trypanosomal activities of DNA topoisomerase inhibitors
    Alexander Deterding
    School of Biological Sciences, University of Bristol, Woodland Road, Bristol BS8 1UG, United Kingdom
    Acta Trop 93:311-6. 2005
    ..These data support the use of DNA topoisomerase inhibitors as lead compounds for anti-trypanosomal drug development...
  43. ncbi request reprint Real-time PCR for detection of Trypanosoma brucei in human blood samples
    Sven Becker
    School of Biological Sciences, University of Bristol, Bristol, United Kingdom
    Diagn Microbiol Infect Dis 50:193-9. 2004
    ..The results indicate that the real-time PCR assay described is a rapid and sensitive method suitable for the detection of T. brucei in human blood samples in routine clinical laboratory practice...
  44. ncbi request reprint Trypanosoma brucei: in vitro slender-to-stumpy differentiation of culture-adapted, monomorphic bloodstream forms
    Tanja Breidbach
    Abteilung Parasitologie, Hygiene Institut der Ruprecht Karls Universität, Im Neuenheimer Feld, Heidelberg, Germany
    Exp Parasitol 101:223-30. 2002
    ..Treatment of monomorphic bloodstream trypanosomes with pCPTcAMP could be a useful method for identifying the genes involved in the slender-to-stumpy differentiation process...
  45. doi request reprint In vitro effect of DNA topoisomerase inhibitors on Candida albicans
    Shing C Kwok
    Biomedical Research Centre, School of Medicine, Health Policy and Practice, University of East Anglia, Norwich
    Med Mycol 48:155-60. 2010
    ..The results of this study indicate that some DNA topoisomerase inhibitors effect the growth and morphology of C. albicans suggesting a possible role as antifungal agents in the treatment of C. albicans infections...
  46. pmc Bis-acridines as lead antiparasitic agents: structure-activity analysis of a discrete compound library in vitro
    Conor R Caffrey
    Sandler Center for Basic Research in Parasitic Diseases, BH508, University of California, San Francisco, 1700 4th Street, San Francisco, CA 94158 2330, USA
    Antimicrob Agents Chemother 51:2164-72. 2007
    ..Our approach illustrates the usefulness of screening focused compound libraries against multiple parasite targets. Some of the bis-acridines identified here may represent useful starting points for further lead optimization...
  47. ncbi request reprint Impaired dimerization and trafficking of ESAG6 lacking a glycosyl-phosphatidylinositol anchor
    Susanne Biebinger
    Zentrum für Molekular Biologie der Universität Heidelberg, Im Nevenheimer Feld 282, 69120, Heidelberg, Germany
    Mol Biochem Parasitol 132:93-6. 2003
  48. ncbi request reprint Screening of acyl hydrazide proteinase inhibitors for antiparasitic activity against Trypanosoma brucei
    Conor R Caffrey
    Department of Pathology, Tropical Disease Research Unit, University of California San Francisco, San Francisco, CA 94143, USA
    Int J Antimicrob Agents 19:227-31. 2002
    ..Nevertheless, the data support the potential of acyl hydrazides as antitrypanosomal chemotherapeutic agents for treatment of sleeping sickness...
  49. ncbi request reprint Intracellular positioning of isoforms explains an unusually large adenylate kinase gene family in the parasite Trypanosoma brucei
    Michael L Ginger
    Sir William Dunn School of Pathology, University of Oxford, South Parks Road, Oxford, OX1 3RE, United Kingdom
    J Biol Chem 280:11781-9. 2005
    ..The anchoring of specific adenylate kinases within two distinct flagellar structures provides a paradigm for metabolic organization and efficiency in other flagellates...