Peter Bütikofer

Summary

Affiliation: University of Bern
Country: Switzerland

Publications

  1. doi request reprint Lipid remodelling of glycosylphosphatidylinositol (GPI) glycoconjugates in procyclic-form trypanosomes: biosynthesis and processing of GPIs revisited
    Peter Bütikofer
    Institute of Biochemistry and Molecular Medicine, University of Bern, Buhlstrasse 28, 3012 Bern, Switzerland
    Biochem J 428:409-18. 2010
  2. ncbi request reprint Phosphorylation of a major GPI-anchored surface protein of Trypanosoma brucei during transport to the plasma membrane
    P Butikofer
    Institutes of Biochemistry and Molecular Biology, General Microbiology, and Parasitology, University of Bern, Bern, Switzerland
    J Cell Sci 112:1785-95. 1999
  3. ncbi request reprint Glycosylphosphatidylinositol-anchored surface molecules of Trypanosoma congolense insect forms are developmentally regulated in the tsetse fly
    Peter Bütikofer
    Institute of Biochemistry and Molecular Biology, University of Bern, Buhlstrasse 28, 3012, Bern, Switzerland
    Mol Biochem Parasitol 119:7-16. 2002
  4. ncbi request reprint Characterisation and cellular localisation of a GPEET procyclin precursor in Trypanosoma brucei insect forms
    Peter Bütikofer
    Institute of Biochemistry and Molecular Biology, University of Bern, Buhlstrasse 28, 3012, Bern, Switzerland
    Mol Biochem Parasitol 119:87-95. 2002
  5. ncbi request reprint Phosphorylation of GPEET procyclin is not necessary for survival of Trypanosoma brucei procyclic forms in culture and in the tsetse fly midgut
    Peter Bütikofer
    Institute of Biochemistry and Molecular Biology, University of Bern, 3012 Bern, Switzerland
    Mol Biochem Parasitol 126:287-91. 2003
  6. ncbi request reprint Coordinate expression of GPEET procyclin and its membrane-associated kinase in Trypanosoma brucei procyclic forms
    Anne Catherine Schlaeppi
    Institute of Biochemistry and Molecular Biology, University of Bern, 3012 Bern, Switzerland
    J Biol Chem 278:49980-7. 2003
  7. pmc Major surface glycoproteins of insect forms of Trypanosoma brucei are not essential for cyclical transmission by tsetse
    Erik Vassella
    Institut fur Zellbiologie, Universitat Bern, Bern, Switzerland
    PLoS ONE 4:e4493. 2009
  8. pmc Trypanosoma congolense procyclins: unmasking cryptic major surface glycoproteins in procyclic forms
    Silvia Utz
    Institute of Biochemistry and Molecular Medicine, University of Bern, Bern, Switzerland
    Eukaryot Cell 5:1430-40. 2006
  9. doi request reprint The protease resistant surface (PRS) glycoconjugate from Trypanosoma congolense has an inositol-acylated glycosylphosphatidylinositol anchor, containing a significant proportion of myristate at the sn-2 position
    Eva Greganova
    Institute of Biochemistry and Molecular Medicine, University of Bern, Buhlstrasse 28, 3012 Bern, Switzerland
    Mol Biochem Parasitol 171:50-4. 2010
  10. doi request reprint The ins and outs of phosphatidylethanolamine synthesis in Trypanosoma brucei
    Luce Farine
    Institute of Biochemistry and Molecular Medicine, University of Bern, 3012 Bern, Switzerland
    Biochim Biophys Acta 1831:533-42. 2013

Collaborators

Detail Information

Publications32

  1. doi request reprint Lipid remodelling of glycosylphosphatidylinositol (GPI) glycoconjugates in procyclic-form trypanosomes: biosynthesis and processing of GPIs revisited
    Peter Bütikofer
    Institute of Biochemistry and Molecular Medicine, University of Bern, Buhlstrasse 28, 3012 Bern, Switzerland
    Biochem J 428:409-18. 2010
    ..To our knowledge, this is the first demonstration of lipid remodelling at the level of protein- or polysaccharide-linked GPI anchors in procyclic-form trypanosomes...
  2. ncbi request reprint Phosphorylation of a major GPI-anchored surface protein of Trypanosoma brucei during transport to the plasma membrane
    P Butikofer
    Institutes of Biochemistry and Molecular Biology, General Microbiology, and Parasitology, University of Bern, Bern, Switzerland
    J Cell Sci 112:1785-95. 1999
    ..To the best of our knowledge, this study represents the first localization of phosphorylated and unphosphorylated forms of a GPI-anchored protein within a cell...
  3. ncbi request reprint Glycosylphosphatidylinositol-anchored surface molecules of Trypanosoma congolense insect forms are developmentally regulated in the tsetse fly
    Peter Bütikofer
    Institute of Biochemistry and Molecular Biology, University of Bern, Buhlstrasse 28, 3012, Bern, Switzerland
    Mol Biochem Parasitol 119:7-16. 2002
    ..Unexpectedly, 14 days post-infection, procyclic forms frequently are negative for both PRS and GARP, suggesting that they might be expressing another stage-specific surface antigen at this point in the life cycle...
  4. ncbi request reprint Characterisation and cellular localisation of a GPEET procyclin precursor in Trypanosoma brucei insect forms
    Peter Bütikofer
    Institute of Biochemistry and Molecular Biology, University of Bern, Buhlstrasse 28, 3012, Bern, Switzerland
    Mol Biochem Parasitol 119:87-95. 2002
    ..The results indicate that the 20 kDa form represents a biosynthetic precursor of GPEET, which has just started to receive components of the poly-N-acetyllactosamine repeat of the GPI anchor...
  5. ncbi request reprint Phosphorylation of GPEET procyclin is not necessary for survival of Trypanosoma brucei procyclic forms in culture and in the tsetse fly midgut
    Peter Bütikofer
    Institute of Biochemistry and Molecular Biology, University of Bern, 3012 Bern, Switzerland
    Mol Biochem Parasitol 126:287-91. 2003
  6. ncbi request reprint Coordinate expression of GPEET procyclin and its membrane-associated kinase in Trypanosoma brucei procyclic forms
    Anne Catherine Schlaeppi
    Institute of Biochemistry and Molecular Biology, University of Bern, 3012 Bern, Switzerland
    J Biol Chem 278:49980-7. 2003
    ..Together, the results demonstrate that GPEET and its kinase are expressed during the same life cycle stages and that factors that induce the expression of GPEET in vitro also induce the expression of the GPEET kinase...
  7. pmc Major surface glycoproteins of insect forms of Trypanosoma brucei are not essential for cyclical transmission by tsetse
    Erik Vassella
    Institut fur Zellbiologie, Universitat Bern, Bern, Switzerland
    PLoS ONE 4:e4493. 2009
    ....
  8. pmc Trypanosoma congolense procyclins: unmasking cryptic major surface glycoproteins in procyclic forms
    Silvia Utz
    Institute of Biochemistry and Molecular Medicine, University of Bern, Bern, Switzerland
    Eukaryot Cell 5:1430-40. 2006
    ..brucei. Using an antiserum raised against the EPGENGT repeat, we show that T. congolense procyclins are expressed continuously in the fly midgut and thus form the surface coat of cells that are negative for both PRS and GARP...
  9. doi request reprint The protease resistant surface (PRS) glycoconjugate from Trypanosoma congolense has an inositol-acylated glycosylphosphatidylinositol anchor, containing a significant proportion of myristate at the sn-2 position
    Eva Greganova
    Institute of Biochemistry and Molecular Medicine, University of Bern, Buhlstrasse 28, 3012 Bern, Switzerland
    Mol Biochem Parasitol 171:50-4. 2010
    ..In addition, we found that PRS is highly rich in galactose and sialic acid residues, suggesting that it may represent a major acceptor of the parasite trans-sialidase...
  10. doi request reprint The ins and outs of phosphatidylethanolamine synthesis in Trypanosoma brucei
    Luce Farine
    Institute of Biochemistry and Molecular Medicine, University of Bern, 3012 Bern, Switzerland
    Biochim Biophys Acta 1831:533-42. 2013
    ..This article is part of a Special Issue entitled Phospholipids and Phospholipid Metabolism...
  11. pmc An essential bacterial-type cardiolipin synthase mediates cardiolipin formation in a eukaryote
    Mauro Serricchio
    Institute of Biochemistry and Molecular Medicine, University of Bern, 3012 Bern, Switzerland
    Proc Natl Acad Sci U S A 109:E954-61. 2012
    ..During depletion of cardiolipin synthase, the levels of cytochrome oxidase subunit IV and cytochrome c1, reflecting mitochondrial respiratory complexes IV and III, respectively, decreased progressively...
  12. pmc Arginine and Lysine Transporters Are Essential for Trypanosoma brucei
    Christoph Mathieu
    Institute of Plant Sciences, University of Bern, Bern, Switzerland
    PLoS ONE 12:e0168775. 2017
    ..Single and double RNAi lines indicate that additional low affinity uptake systems for arginine and lysine are present in T. brucei...
  13. doi request reprint Characterization of choline uptake in Trypanosoma brucei procyclic and bloodstream forms
    Juan P Macedo
    Institute of Biochemistry and Molecular Medicine, University of Bern, 3012 Bern, Switzerland
    Mol Biochem Parasitol 190:16-22. 2013
    ..Together, our results show that T. brucei parasites express an uptake system for choline and that exogenous choline is used for PC synthesis. ..
  14. pmc myo-Inositol uptake is essential for bulk inositol phospholipid but not glycosylphosphatidylinositol synthesis in Trypanosoma brucei
    Amaia González-Salgado
    Institute of Biochemistry and Molecular Medicine, University of Bern, Buhlstrasse 28, 3012 Bern, Switzerland
    J Biol Chem 287:13313-23. 2012
    ..This together with the unexpected localization of the myo-inositol transporter in both the plasma membrane and the Golgi demonstrate that metabolism of endogenous and exogenous myo-inositol in T. brucei is strictly segregated...
  15. doi request reprint Phosphatidylglycerophosphate synthase associates with a mitochondrial inner membrane complex and is essential for growth of Trypanosoma brucei
    Mauro Serricchio
    Institute of Biochemistry and Molecular Medicine, University of Bern, Bern, Switzerland
    Mol Microbiol 87:569-79. 2013
    ..brucei cardiolipin synthase and cytochrome c1, a protein of respiratory complex III...
  16. doi request reprint Trypanosoma brucei: a model micro-organism to study eukaryotic phospholipid biosynthesis
    Mauro Serricchio
    Institute of Biochemistry and Molecular Medicine, University of Bern, Bern, Switzerland
    FEBS J 278:1035-46. 2011
    ..brucei represents the only eukaryote so far that synthesizes all three sphingophospholipid classes, sphingomyelin, inositolphosphorylceramide and ethanolaminephosphorylceramide, and that their production is developmentally regulated...
  17. pmc Flagellar membranes are rich in raft-forming phospholipids
    Mauro Serricchio
    Institute of Biochemistry and Molecular Medicine, University of Bern, Bern 3012, Switzerland
    Biol Open 4:1143-53. 2015
    ..Our study provides direct evidence for a preferential presence of raft-forming phospholipids in flagellar membranes of T. brucei. ..
  18. doi request reprint A heteromeric potassium channel involved in the modulation of the plasma membrane potential is essential for the survival of African trypanosomes
    Michael E Steinmann
    Institute of Biochemistry and Molecular Medicine, University of Bern, Bern, Switzerland Swiss Tropical and Public Health Institute, Basel, Switzerland and University of Basel, Basel, Switzerland
    FASEB J 29:3228-37. 2015
    ..Thus, this heteromeric potassium channel is involved in the modulation of the plasma membrane potential and represents a novel drug target in T. brucei...
  19. pmc Eukaryotic translation elongation factor 1A (eEF1A) domain I from S. cerevisiae is required but not sufficient for inter-species complementation
    Sandra Eltschinger
    Institute of Biochemistry and Molecular Medicine, University of Bern, Bern, Switzerland
    PLoS ONE 7:e42338. 2012
    ..However, functional complementation in yeast can be obtained using eEF1A chimera containing domains II or III from other species. In contrast, yeast domain I is strictly required for functional complementation in S. cerevisiae...
  20. pmc RFT1 affects GPI anchor glycosylation
    Petra Gottier
    University of Bern, Switzerland
    J Biol Chem . 2016
    ..Our results implicate RFT1 in a wider range of glycosylation processes than previously appreciated...
  21. pmc Phosphatidylethanolamine and phosphatidylcholine biosynthesis by the Kennedy pathway occurs at different sites in Trypanosoma brucei
    Luce Farine
    Institute of Biochemistry and Molecular Medicine, University of Bern, 3012 Bern, Switzerland
    Sci Rep 5:16787. 2015
    ..In contrast, TbCEPT was detected in the bulk ER. ..
  22. pmc An Atypical Mitochondrial Carrier That Mediates Drug Action in Trypanosoma brucei
    Juan P de Macêdo
    Institute of Biochemistry and Molecular Medicine, University of Bern, Bern, Switzerland Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland
    PLoS Pathog 11:e1004875. 2015
    ..Since TbMCP14 belongs to a trypanosomatid-specific clade of mitochondrial carrier family proteins showing very poor similarity to mitochondrial carriers of mammals, it may represent an interesting target for drug action or targeting. ..
  23. pmc A structural domain mediates attachment of ethanolamine phosphoglycerol to eukaryotic elongation factor 1A in Trypanosoma brucei
    Eva Greganova
    Institute of Biochemistry and Molecular Medicine, University of Bern, Bern, Switzerland
    PLoS ONE 5:e9486. 2010
    ..Using T. brucei as a model organism, we show that amino acid substitutions around the modification site are not critical for EPG attachment and that a truncated version of domain III of eEF1A is sufficient to mediate EPG addition...
  24. doi request reprint Perturbation of phosphatidylethanolamine synthesis affects mitochondrial morphology and cell-cycle progression in procyclic-form Trypanosoma brucei
    Aita Signorell
    Institute of Biochemistry and Molecular Medicine, University of Bern, 3012 Bern, Switzerland
    Mol Microbiol 72:1068-79. 2009
    ..In contrast, RNAi against choline-/ethanolamine phosphotransferase, which affected PC as well as PE levels, caused a cell division phenotype characterized by non-division of the nucleus and production of zoids...
  25. pmc Trypanosoma brucei Bloodstream Forms Depend upon Uptake of myo-Inositol for Golgi Complex Phosphatidylinositol Synthesis and Normal Cell Growth
    Amaia González-Salgado
    Institute of Biochemistry and Molecular Medicine, University of Bern, Bern, Switzerland
    Eukaryot Cell 14:616-24. 2015
    ..It tolerates only a single modification on the inositol ring, such as the removal of a hydroxyl group or the inversion of stereochemistry at a single hydroxyl group relative to myo-inositol. ..
  26. doi request reprint Trypanosoma brucei eflornithine transporter AAT6 is a low-affinity low-selective transporter for neutral amino acids
    Christoph Mathieu
    Institute of Plant Sciences, University of Bern, Altenbergrain 21, 3013 Bern, Switzerland
    Biochem J 463:9-18. 2014
    ....
  27. pmc Phosphatidylethanolamine in Trypanosoma brucei is organized in two separate pools and is synthesized exclusively by the Kennedy pathway
    Aita Signorell
    Institute of Biochemistry and Molecular Medicine, University of Bern, Buhlstrasse 28, Bern, Switzerland
    J Biol Chem 283:23636-44. 2008
    ..In addition, we show that phosphatidylserine in T. brucei procyclic forms is synthesized exclusively by base exchange with phosphatidylethanolamine...
  28. pmc Characterisation of gp34, a GPI-anchored protein expressed by schizonts of Theileria parva and T. annulata
    Gondga Xue
    Division of Molecular Pathobiology, DCR VPH, Vetsuisse Faculty, University of Bern, CH 3013 Bern, Switzerland
    Mol Biochem Parasitol 172:113-20. 2010
    ..Overexpression of Tp-gp34 and Ta-gp34 induced cytokinetic defects and resulted in accumulation of binucleated cells. These findings suggest that gp34 could contribute to important parasite-host interactions during host cell division...
  29. doi request reprint Phosphatidylethanolamine is the precursor of the ethanolamine phosphoglycerol moiety bound to eukaryotic elongation factor 1A
    Aita Signorell
    Institute of Biochemistry and Molecular Medicine, University of Bern, Bern, Switzerland
    J Biol Chem 283:20320-9. 2008
    ..brucei procyclic forms and, concomitantly, in a block in glycosylphosphatidylinositol attachment to procyclins and ethanolamine-phosphoglycerol modification of eukaryotic elongation factor 1A...
  30. pmc A Glycosylation Mutant of Trypanosoma brucei Links Social Motility Defects In Vitro to Impaired Colonization of Tsetse Flies In Vivo
    Simon Imhof
    Institute of Cell Biology, University of Bern, Bern, Switzerland Graduate School of Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland
    Eukaryot Cell 14:588-92. 2015
    ....
  31. pmc Autophagy in Trypanosoma brucei: amino acid requirement and regulation during different growth phases
    Remo S Schmidt
    Institute of Biochemistry and Molecular Medicine, University of Bern, Bern, Switzerland Graduate School for Cellular and Biomedical Sciences, University of Bern, Bern, Switzerland
    PLoS ONE 9:e93875. 2014
    ..In addition, we show that autophagy is induced when parasites enter stationary growth phase in culture and that their capacity to undergo starvation-induced autophagy decreases with increasing cell density. ..
  32. ncbi request reprint Anti-mouse GPI-PLD antisera highlight structural differences between murine and bovine GPI-PLDs
    Patrick Gregory
    Institute of Biochemistry and Molecular Biology, University of Bern, Buhlstrasse 28, CH 3012 Bern, Switzerland
    Biol Chem 384:1575-82. 2003
    ..We discuss the implications of these results and stress the importance of antibody selection while investigating GPI-PLD in the mouse...