Thomas Seebeck

Summary

Affiliation: University of Bern
Country: Switzerland

Publications

  1. ncbi request reprint cAMP signalling in the kinetoplastid protozoa
    T Seebeck
    Institute of Cell Biology, University of Bern, Baltzerstrasse 4, CH 3012 Bern, Switzerland
    Curr Mol Med 4:585-99. 2004
  2. pmc Pharmacological validation of Trypanosoma brucei phosphodiesterases as novel drug targets
    Harry P de Koning
    Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, United Kingdom
    J Infect Dis 206:229-37. 2012
  3. ncbi request reprint cAMP signalling in Trypanosoma brucei
    T Seebeck
    Institute of Cell Biology, University of Bern, Baltzerstrasse 4, CH 3012, Bern, Switzerland
    Int J Parasitol 31:491-8. 2001
  4. pmc Phosphodiesterase inhibitors as a new generation of antiprotozoan drugs: exploiting the benefit of enzymes that are highly conserved between host and parasite
    Thomas Seebeck
    Institute of Cell Biology, University of Bern, Baltzerstrasse 4, CH 3012 Bern, Switzerland
    Future Med Chem 3:1289-306. 2011
  5. ncbi request reprint A FYVE-containing unusual cyclic nucleotide phosphodiesterase from Trypanosoma cruzi
    Stefan Kunz
    Institute of Cell Biology, University of Bern, Bern, Switzerland
    FEBS J 272:6412-22. 2005
  6. pmc The N terminus of phosphodiesterase TbrPDEB1 of Trypanosoma brucei contains the signal for integration into the flagellar skeleton
    Edith Luginbuehl
    Institute of Cell Biology, University of Bern, Switzerland
    Eukaryot Cell 9:1466-75. 2010
  7. pmc Gene conversion transfers the GAF-A domain of phosphodiesterase TbrPDEB1 to one allele of TbrPDEB2 of Trypanosoma brucei
    Stefan Kunz
    Institute of Cell Biology, University of Bern, Bern, Switzerland
    PLoS Negl Trop Dis 3:e455. 2009
  8. doi request reprint Cyclic nucleotide-specific phosphodiesterases of Plasmodium falciparum: PfPDEalpha, a non-essential cGMP-specific PDE that is an integral membrane protein
    Laurent Wentzinger
    Institute of Cell Biology, University of Bern, Switzerland
    Int J Parasitol 38:1625-37. 2008
  9. pmc Cyclic nucleotide specific phosphodiesterases of Leishmania major
    Andrea Johner
    Institute for Cell Biology, University of Bern, Baltzerstrasse 4, CH 3012 Bern, Switzerland
    BMC Microbiol 6:25. 2006
  10. pmc The exopolyphosphatase TbrPPX1 of Trypanosoma brucei
    Edith Luginbuehl
    Institute of Cell Biology, University of Bern, Baltzerstrasse 4, CH 3012 Bern, Switzerland
    BMC Microbiol 11:4. 2011

Collaborators

Detail Information

Publications35

  1. ncbi request reprint cAMP signalling in the kinetoplastid protozoa
    T Seebeck
    Institute of Cell Biology, University of Bern, Baltzerstrasse 4, CH 3012 Bern, Switzerland
    Curr Mol Med 4:585-99. 2004
    ..Homologues of protein kinase A and its regulatory subunits have been identified, but their biochemical properties seem to be disctinct from that of mammalian protein kinase A...
  2. pmc Pharmacological validation of Trypanosoma brucei phosphodiesterases as novel drug targets
    Harry P de Koning
    Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, United Kingdom
    J Infect Dis 206:229-37. 2012
    ..Cpd A prevents cytokinesis, resulting in multinucleated, multiflagellated cells that eventually lyse. These observations pharmacologically validate the highly conserved trypanosomal PDEs as potential drug targets...
  3. ncbi request reprint cAMP signalling in Trypanosoma brucei
    T Seebeck
    Institute of Cell Biology, University of Bern, Baltzerstrasse 4, CH 3012, Bern, Switzerland
    Int J Parasitol 31:491-8. 2001
    ..This protein exhibits considerable similarity with its mammalian counterparts. Immunoprecipitation co-precipitates a protein kinase activity with the characteristics of protein kinase A...
  4. pmc Phosphodiesterase inhibitors as a new generation of antiprotozoan drugs: exploiting the benefit of enzymes that are highly conserved between host and parasite
    Thomas Seebeck
    Institute of Cell Biology, University of Bern, Baltzerstrasse 4, CH 3012 Bern, Switzerland
    Future Med Chem 3:1289-306. 2011
    ..Given the high degree of structural similarity between the human and the protozoan PDEs, the vast expertise available in the human field can now be applied to developing disease-specific PDE inhibitors as new antiprotozoan drugs...
  5. ncbi request reprint A FYVE-containing unusual cyclic nucleotide phosphodiesterase from Trypanosoma cruzi
    Stefan Kunz
    Institute of Cell Biology, University of Bern, Bern, Switzerland
    FEBS J 272:6412-22. 2005
    ..TcrPDEC can complement a PDE-deficient yeast strain. Unexpectedly for a kinetoplastid PDE, TcrPDEC is a dual-specificity PDE that accepts both cAMP and cGMP as its substrates...
  6. pmc The N terminus of phosphodiesterase TbrPDEB1 of Trypanosoma brucei contains the signal for integration into the flagellar skeleton
    Edith Luginbuehl
    Institute of Cell Biology, University of Bern, Switzerland
    Eukaryot Cell 9:1466-75. 2010
    ..Mutagenesis of selected residues in the N-terminal region of PDEB2 demonstrated that single amino acid changes are sufficient to redirect the reporter from a cell body location to stable integration into the flagellar skeleton...
  7. pmc Gene conversion transfers the GAF-A domain of phosphodiesterase TbrPDEB1 to one allele of TbrPDEB2 of Trypanosoma brucei
    Stefan Kunz
    Institute of Cell Biology, University of Bern, Bern, Switzerland
    PLoS Negl Trop Dis 3:e455. 2009
    ..They are separated by 2379 bp, and both code for phosphodiesterases with two GAF domains in their N-terminal moieties and a catalytic domain at the C-terminus...
  8. doi request reprint Cyclic nucleotide-specific phosphodiesterases of Plasmodium falciparum: PfPDEalpha, a non-essential cGMP-specific PDE that is an integral membrane protein
    Laurent Wentzinger
    Institute of Cell Biology, University of Bern, Switzerland
    Int J Parasitol 38:1625-37. 2008
    ..PfPDEalpha was characterized as a cGMP-specific PDE that is not sensitive to a number of standard PDE inhibitors. Genetic ablation of the PfPDE1 gene produced no major phenotype in erythrocyte cultures...
  9. pmc Cyclic nucleotide specific phosphodiesterases of Leishmania major
    Andrea Johner
    Institute for Cell Biology, University of Bern, Baltzerstrasse 4, CH 3012 Bern, Switzerland
    BMC Microbiol 6:25. 2006
    ....
  10. pmc The exopolyphosphatase TbrPPX1 of Trypanosoma brucei
    Edith Luginbuehl
    Institute of Cell Biology, University of Bern, Baltzerstrasse 4, CH 3012 Bern, Switzerland
    BMC Microbiol 11:4. 2011
    ..Earlier work has suggested that a human exopolyphosphatase, Prune, might exhibit cyclic nucleotide phosphodiesterase activity...
  11. ncbi request reprint Cyclic nucleotide specific phosphodiesterases of the kinetoplastida: a unified nomenclature
    Stefan Kunz
    Institute of Cell Biology, University of Bern, Baltzerstrasse 4, CH 3012 Bern, Switzerland
    Mol Biochem Parasitol 145:133-5. 2006
  12. ncbi request reprint The Trypanosoma brucei cAMP phosphodiesterases TbrPDEB1 and TbrPDEB2: flagellar enzymes that are essential for parasite virulence
    Michael Oberholzer
    Institute of Cell Biology, University of Bern, Switzerland
    FASEB J 21:720-31. 2007
    ..Furthermore, they are compatible with the notion that the flagellum of T. brucei is an important site of cAMP signaling...
  13. ncbi request reprint Trypanosomes and mammalian sperm: one of a kind?
    Michael Oberholzer
    Institute of Cell Biology, University of Bern, CH 3012 Bern, Switzerland
    Trends Parasitol 23:71-7. 2007
    ..Recent proteome projects studying the flagella of mammalian sperm and kinetoplastid parasites have provided important information and have indicated a surprising degree of similarities between the flagella of these two cell types...
  14. doi request reprint Mitochondrial translation is essential in bloodstream forms of Trypanosoma brucei
    Marina Cristodero
    Department of Chemistry and Biochemistry, University of Bern, Freiestr 3, CH 3012 Bern, Switzerland
    Mol Microbiol 78:757-69. 2010
    ..Both of these proteins show trypanosomatid-specific features and may therefore be excellent novel drug targets...
  15. ncbi request reprint A vector series for rapid PCR-mediated C-terminal in situ tagging of Trypanosoma brucei genes
    Michael Oberholzer
    Institute of Cell Biology, University of Bern, Baltzerstrasse 4, CH 3012 Bern, Switzerland
    Mol Biochem Parasitol 145:117-20. 2006
  16. ncbi request reprint TbPDE1, a novel class I phosphodiesterase of Trypanosoma brucei
    Stefan Kunz
    Institute of Cell Biology, University of Bern, Switzerland
    Eur J Biochem 271:637-47. 2004
    ..A fragment of TbPDE1 containing the catalytic domain could be expressed in active form in Escherichia coli. The recombinant enzyme is specific for cAMP, but exhibits a remarkably high Km of > 600 microm for this substrate...
  17. pmc The cAMP-specific phosphodiesterase TbPDE2C is an essential enzyme in bloodstream form Trypanosoma brucei
    Roya Zoraghi
    Institute of Cell Biology, University of Bern, Baltzerstrasse 4, CH 3012 Bern, Switzerland
    Proc Natl Acad Sci U S A 99:4343-8. 2002
    ..TbPDE2C might represent a novel drug target for the development of new and effective trypanocidal drugs...
  18. ncbi request reprint Molecular pharmacology of adenosine transport in Trypanosoma brucei: P1/P2 revisited
    Federico Geiser
    Institute of Cell Biology, Baltzerstrasse 4, CH 3012 Bern, Switzerland
    Mol Pharmacol 68:589-95. 2005
    ..Overexpression of P1 by trypanosomes after loss of P2 indicates that combinatorial chemotherapy with trypanocidal P1 and P2 substrates may be a promising strategy to prevent drug resistance in sleeping sickness...
  19. pmc A venom-derived neurotoxin, CsTx-1, from the spider Cupiennius salei exhibits cytolytic activities
    Lucia Kuhn-Nentwig
    Institute of Ecology and Evolution, University of Bern, Baltzerstrasse 6, CH 3012 Bern, Switzerland
    J Biol Chem 287:25640-9. 2012
    ..Such a dualism is discussed as an important new mechanism for the evolution of spider venomous peptides...
  20. doi request reprint Cupiennin 1a exhibits a remarkably broad, non-stereospecific cytolytic activity on bacteria, protozoan parasites, insects, and human cancer cells
    Lucia Kuhn-Nentwig
    Institute of Ecology and Evolution, University of Bern, Baltzerstrasse 6, 3012, Bern, Switzerland
    Amino Acids 40:69-76. 2011
    ..Reducing the overall negative charges of erythrocytes by partially removing their sialic acids or by protecting them with tri- or pentalysine results in reduced haemolytic activity of the peptide...
  21. pmc Mechanisms of arsenical and diamidine uptake and resistance in Trypanosoma brucei
    Enock Matovu
    Institute of Cell Biology, CH 3012 Bern, Switzerland
    Eukaryot Cell 2:1003-8. 2003
    ..High-level arsenical resistance therefore appears to involve the loss of more than one transporter...
  22. ncbi request reprint The major components of the paraflagellar rod of Trypanosoma brucei are two similar, but distinct proteins which are encoded by two different gene loci
    J Deflorin
    Institute for General Microbiology, University of Bern, Switzerland
    J Biol Chem 269:28745-51. 1994
    ..This unusual organization leads to the generation of mRNAs which contain identical coding sequences but different 5'- and 3'-noncoding regions...
  23. ncbi request reprint The repetitive microtubule-associated proteins MARP-1 and MARP-2 of Trypanosoma brucei
    M Affolter
    Institut fur Allgemeine Mikrobiologie, University of Bern, Switzerland
    J Struct Biol 112:241-51. 1994
    ..This C-terminus does not show sequence similarity with any other microtubule-associated proteins and thus appears to represent a novel type of microtubule-binding domain...
  24. ncbi request reprint Identification of a profilin homologue in Trypanosoma brucei by complementation screening
    W Wilson
    Institut fur Allgemeine Mikrobiologie, Universitat Bern, Switzerland
    Gene 187:201-9. 1997
    ..1 kDa. The gene appears to be present at single copy and is expressed at approximately equal levels in both mammalian and insect forms of the parasite...
  25. ncbi request reprint Cyclic AMP signaling in trypanosomatids
    C Naula
    University of Bern, Institute of General Microbiology, Switzerland
    Parasitol Today 16:35-8. 2000
    ..Here, Christina Naula and Thomas Seebeck summarize what is known about cAMP signal transduction in trypanosomatids.
  26. ncbi request reprint Nucleoside diphosphate kinase of Trypanosoma brucei
    I Hunger-Glaser
    Institute of Cell Biology, University of Bern, Baltzerstrasse 4, CH 3012, Bern, Switzerland
    Gene 257:251-7. 2000
    ..Histidine phosphorylation of TbNDPK is essentially resistant to the experimental compound LY266500, a potent inhibitor of histidine phosphorylation of trypanosomal succinyl coenzyme A synthase...
  27. ncbi request reprint The regulatory subunit of a cGMP-regulated protein kinase A of Trypanosoma brucei
    T Shalaby
    Institute of Cell Biology, University of Bern, Switzerland
    Eur J Biochem 268:6197-206. 2001
    ..Binding studies with recombinant cyclic nucleotide binding domains of TbRSU confirmed that both domains bind cGMP with Kd values in the lower micromolar range, and that up to a 100-fold excess of cAMP does not compete with cGMP binding...
  28. ncbi request reprint Characterization of TbPDE2A, a novel cyclic nucleotide-specific phosphodiesterase from the protozoan parasite Trypanosoma brucei
    R Zoraghi
    Institute for Cell Biology, University of Bern, Baltzerstrasse 4, Berne CH-3012, Switzerland
    J Biol Chem 276:11559-66. 2001
    ..4, 5.9, 9.4, and 14.2 micrometer, respectively. All four compounds inhibit proliferation of bloodstream form trypanosomes in culture, indicating that TbPDE2A is an essential enzyme...
  29. ncbi request reprint Spontaneous dimerization and leucine-zipper induced activation of the recombinant catalytic domain of a new adenylyl cyclase of Trypanosoma brucei, GRESAG4.4B
    C Naula
    Institute for Cell Biology, University of Bern, Baltzerstrasse 4, CH 3012 Bern, Switzerland
    Mol Biochem Parasitol 112:19-28. 2001
    ..The LZ appears to enforce a distinct conformation of the dimer, which leads to an increased enzymatic activity, and thus may mimic the effect of ligand-induced dimerization of adenylyl cyclase in vivo...
  30. ncbi request reprint Melarsoprol refractory T. b. gambiense from Omugo, north-western Uganda
    E Matovu
    Livestock Health Research Institute, Tororo, Uganda
    Trop Med Int Health 6:407-11. 2001
    ..From our findings it appears that reduced drug susceptibility may be one factor for the frequent relapses of sleeping sickness after melarsoprol treatment occurring in Northwest Uganda...
  31. ncbi request reprint The Trypanosoma brucei autoantigen I/6 is an internally repetitive cytoskeletal protein
    E Detmer
    Institut fur Allgemeine Mikrobiologie, Universitat Bern, Switzerland
    Eur J Cell Biol 72:378-84. 1997
    ..Protein I/6 contains a non-functional EF-hand calcium binding domain and a domain of six tandemly arranged repeat units of eight amino acids length...
  32. ncbi request reprint Drug resistance in Trypanosoma brucei spp., the causative agents of sleeping sickness in man and nagana in cattle
    E Matovu
    Livestock Health Research Institute, P.O. Box 96, Tororo, Uganda
    Microbes Infect 3:763-70. 2001
    ..Understanding these mechanisms at the molecular level will enable improved management of existing drugs and provide valuable clues to the development of new trypanocides...
  33. ncbi request reprint Genetic variants of the TbAT1 adenosine transporter from African trypanosomes in relapse infections following melarsoprol therapy
    E Matovu
    Institute of Cell Biology, University of Bern, Baltzerstrasse 4, CH-3012, Bern, Switzerland
    Mol Biochem Parasitol 117:73-81. 2001
    ..They further demonstrate that many isolates from relapse patients contained the wild-type TbAT1 genes, suggesting that melarsoprol refractoriness is not solely due to a mutational inactivation of TbAT1...
  34. ncbi request reprint A glycosomal protein (p60) which is predominantly expressed in procyclic Trypanosoma brucei. Characterization and DNA sequence
    V Kueng
    Institut fur Allgemeine Mikrobiologie, Universitat Bern, Switzerland
    J Biol Chem 264:5203-9. 1989
    ..The biological role of the microtubule-binding capability of p60 remains unclear, whereas its membrane binding may be of physiological significance inside the glycosome...
  35. pmc The major component of the paraflagellar rod of Trypanosoma brucei is a helical protein that is encoded by two identical, tandemly linked genes
    K Schlaeppi
    Institut fur Allgemeine Mikrobiologie, Bern, Switzerland
    J Cell Biol 109:1695-709. 1989
    ..The PFR protein is coded for by two tandemly linked genes of identical nucleotide sequence. Both genes are transcribed into stable mRNAs of very similar length that carry the mini-exon sequence at their 5' termini...