Reuven Agami

Summary

Affiliation: The Netherlands Cancer Institute
Country: The Netherlands

Publications

  1. ncbi request reprint All roads lead to IKKepsilon
    Reuven Agami
    Division of Tumor Biology, The Netherlands Cancer Institute, Amsterdam 1066 CX, The Netherlands
    Cell 129:1043-5. 2007
  2. pmc Myc coordinates transcription and translation to enhance transformation and suppress invasiveness
    Ran Elkon
    Division of Biological Stress Response, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    EMBO Rep 16:1723-36. 2015
  3. doi request reprint Enhancer-associated RNAs as therapeutic targets
    Nicolas Léveillé
    The Netherlands Cancer Institute, Division of Biological Stress Response, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands
    Expert Opin Biol Ther 15:723-34. 2015
  4. pmc Reducing hypothalamic AGRP by RNA interference increases metabolic rate and decreases body weight without influencing food intake
    Hideo Makimura
    Fishberg Center for Neurobiology, Neurobiology of Aging Laboratories, Mount Sinai School of Medicine, One Gustave L, Levy Place, Box 1639, New York, New York 10029, USA
    BMC Neurosci 3:18. 2002
  5. ncbi request reprint RNAi and related mechanisms and their potential use for therapy
    Reuven Agami
    Division of Tumor Biology and Center for Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands
    Curr Opin Chem Biol 6:829-34. 2002
  6. doi request reprint microRNAs, RNA binding proteins and cancer
    Reuven Agami
    Division of Gene Regulation, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    Eur J Clin Invest 40:370-4. 2010
  7. ncbi request reprint A genetic screen implicates miRNA-372 and miRNA-373 as oncogenes in testicular germ cell tumors
    P Mathijs Voorhoeve
    Division of Tumour Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    Adv Exp Med Biol 604:17-46. 2007
  8. doi request reprint BRD7 is a candidate tumour suppressor gene required for p53 function
    Jarno Drost
    The Netherlands Cancer Institute, Division of Gene Regulation, Plesmanlaan 121, 1066CX, Amsterdam, The Netherlands
    Nat Cell Biol 12:380-9. 2010
  9. ncbi request reprint A genetic screen identifies PITX1 as a suppressor of RAS activity and tumorigenicity
    Ingrid G M Kolfschoten
    Division of Tumor Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Cell 121:849-58. 2005
  10. ncbi request reprint Telomerase-independent regulation of ATR by human telomerase RNA
    Martijn Kedde
    Division of Tumor Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066CX Amsterdam, The Netherlands
    J Biol Chem 281:40503-14. 2006

Collaborators

Detail Information

Publications58

  1. ncbi request reprint All roads lead to IKKepsilon
    Reuven Agami
    Division of Tumor Biology, The Netherlands Cancer Institute, Amsterdam 1066 CX, The Netherlands
    Cell 129:1043-5. 2007
    ..Using three complementary genetic approaches, Boehm et al. (2007) identify IKBKE--which encodes IKKepsilon, a component of the NF-kappaB pathway--as a breast cancer oncogene...
  2. pmc Myc coordinates transcription and translation to enhance transformation and suppress invasiveness
    Ran Elkon
    Division of Biological Stress Response, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    EMBO Rep 16:1723-36. 2015
    ..Our results elucidate novel facets of Myc-induced cellular responses and provide a more comprehensive view of the consequences of its activation in cancer cells. ..
  3. doi request reprint Enhancer-associated RNAs as therapeutic targets
    Nicolas Léveillé
    The Netherlands Cancer Institute, Division of Biological Stress Response, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands
    Expert Opin Biol Ther 15:723-34. 2015
    ..Thus, manipulation of eRNA levels becomes a novel and appealing avenue for the design of new therapeutic treatments...
  4. pmc Reducing hypothalamic AGRP by RNA interference increases metabolic rate and decreases body weight without influencing food intake
    Hideo Makimura
    Fishberg Center for Neurobiology, Neurobiology of Aging Laboratories, Mount Sinai School of Medicine, One Gustave L, Levy Place, Box 1639, New York, New York 10029, USA
    BMC Neurosci 3:18. 2002
    ....
  5. ncbi request reprint RNAi and related mechanisms and their potential use for therapy
    Reuven Agami
    Division of Tumor Biology and Center for Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands
    Curr Opin Chem Biol 6:829-34. 2002
    ..These new tools were successfully applied to inhibit tumorigenicity and viral replication. Future development of appropriate in vivo delivery systems may make this technology useful for disease therapy...
  6. doi request reprint microRNAs, RNA binding proteins and cancer
    Reuven Agami
    Division of Gene Regulation, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    Eur J Clin Invest 40:370-4. 2010
    ..Although miRNAs are prominent factors in cancer, little is known about how RBPs affect their cancerous function...
  7. ncbi request reprint A genetic screen implicates miRNA-372 and miRNA-373 as oncogenes in testicular germ cell tumors
    P Mathijs Voorhoeve
    Division of Tumour Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    Adv Exp Med Biol 604:17-46. 2007
    ..We provide evidence that these miRNAs are potential novel oncogenes participating in the development of human testicular germ cell tumors by numbing the p53 pathway, thus allowing tumorigenic growth in the presence of wild-type p53...
  8. doi request reprint BRD7 is a candidate tumour suppressor gene required for p53 function
    Jarno Drost
    The Netherlands Cancer Institute, Division of Gene Regulation, Plesmanlaan 121, 1066CX, Amsterdam, The Netherlands
    Nat Cell Biol 12:380-9. 2010
    ..Thus, BRD7 suppresses tumorigenicity by serving as a p53 cofactor required for the efficient induction of p53-dependent oncogene-induced senescence...
  9. ncbi request reprint A genetic screen identifies PITX1 as a suppressor of RAS activity and tumorigenicity
    Ingrid G M Kolfschoten
    Division of Tumor Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Cell 121:849-58. 2005
    ..Finally, we identified RASAL1, a RAS-GTPase-activating protein, as a transcription target through which PITX1 affects RAS function. Thus, PITX1 suppresses tumorigenicity by downregulating the RAS pathway through RASAL1...
  10. ncbi request reprint Telomerase-independent regulation of ATR by human telomerase RNA
    Martijn Kedde
    Division of Tumor Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066CX Amsterdam, The Netherlands
    J Biol Chem 281:40503-14. 2006
    ..Thus, we uncovered a novel, telomerase-independent, function of hTR that restrains ATR activity and participates in the recovery of cells from UV radiation...
  11. ncbi request reprint RNA-binding protein Dnd1 inhibits microRNA access to target mRNA
    Martijn Kedde
    The Netherlands Cancer Institute, Division of Tumor Biology, Plesmanlaan 121, 1066CX, Amsterdam, The Netherlands
    Cell 131:1273-86. 2007
    ..These effects of Dnd1 are mediated through uridine-rich regions present in the miRNA-targeted mRNAs. Thus, our data unravel a novel role of Dnd1 in protecting certain mRNAs from miRNA-mediated repression...
  12. pmc Selective inhibition of microRNA accessibility by RBM38 is required for p53 activity
    Nicolas Léveillé
    Division of Gene Regulation, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Nat Commun 2:513. 2011
    ..Thus, our results indicate a novel layer of p53 gene regulation, which is required for its tumour suppressive function...
  13. doi request reprint A Pumilio-induced RNA structure switch in p27-3' UTR controls miR-221 and miR-222 accessibility
    Martijn Kedde
    Division of Gene Regulation, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands
    Nat Cell Biol 12:1014-20. 2010
    ..We have therefore uncovered a novel RBP-induced structural switch modulating microRNA-mediated gene expression regulation...
  14. doi request reprint Regulation of the adenomatous polyposis coli gene by the miR-135 family in colorectal cancer
    Remco Nagel
    The Netherlands Cancer Institute, Division of Tumor Biology, Amsterdam, The Netherlands
    Cancer Res 68:5795-802. 2008
    ..Thus, our results uncover a miRNA-mediated mechanism for the control of APC expression and Wnt pathway activity, and suggest its contribution to colorectal cancer pathogenesis...
  15. ncbi request reprint A large-scale RNAi screen in human cells identifies new components of the p53 pathway
    Katrien Berns
    Division of Molecular Carcinogenesis and Center for Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Nature 428:431-7. 2004
    ..Furthermore, we describe siRNA bar-code screens to rapidly identify individual siRNA vectors associated with a specific phenotype. These new tools will greatly facilitate large-scale loss-of-function genetic screens in mammalian cells...
  16. pmc Methylation-mediated silencing and tumour suppressive function of hsa-miR-124 in cervical cancer
    Saskia M Wilting
    Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands
    Mol Cancer 9:167. 2010
    ..Since DNA methylation-based silencing of hsa-miR-124 occurs in various human cancers, we studied the frequency and functional effects of hsa-miR-124 methylation in cervical carcinogenesis...
  17. doi request reprint Alternative cleavage and polyadenylation: extent, regulation and function
    Ran Elkon
    Division of Gene Regulation, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Nat Rev Genet 14:496-506. 2013
    ....
  18. doi request reprint Alternative cleavage and polyadenylation during colorectal cancer development
    Adam R Morris
    The Netherlands Cancer Institute, The Netherlands
    Clin Cancer Res 18:5256-66. 2012
    ....
  19. doi request reprint eRNAs are required for p53-dependent enhancer activity and gene transcription
    Carlos A Melo
    Division of Gene Regulation, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, the Netherlands Doctoral Programme in Biomedicine and Experimental Biology, Centre for Neuroscience and Cell Biology, 3004 517 Coimbra, Portugal
    Mol Cell 49:524-35. 2013
    ..Moreover, eRNA production from p53BERs is required for efficient p53 transcription enhancement...
  20. pmc Regulation of the p27(Kip1) tumor suppressor by miR-221 and miR-222 promotes cancer cell proliferation
    Carlos le Sage
    Division of Tumor Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    EMBO J 26:3699-708. 2007
    ..Thus, deregulated expression of miR-221&222 promotes cancerous growth by inhibiting the expression of p27(Kip1)...
  21. pmc miR-148 targets human DNMT3b protein coding region
    Anja M Duursma
    Division of Tumor Biology, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands
    RNA 14:872-7. 2008
    ..More generally, we provide evidence that coding regions of human genes can be targeted by miRNAs, and that such a mechanism might play a role in determining the relative abundance of different splice variants...
  22. pmc Genome-wide profiling of p53-regulated enhancer RNAs uncovers a subset of enhancers controlled by a lncRNA
    Nicolas Léveillé
    Division of Biological Stress Response, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Nat Commun 6:6520. 2015
    ..Finally, promoter-associated hypermethylation analysis shows silencing of LED in human tumours. Thus, our study identifies a new layer of complexity in the p53 pathway and suggests its dysregulation in cancer. ..
  23. doi request reprint The poly(A)-binding protein nuclear 1 suppresses alternative cleavage and polyadenylation sites
    Mathias Jenal
    Division of Gene Regulation, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Cell 149:538-53. 2012
    ..Our results elucidate a novel function for PABPN1 as a suppressor of APA...
  24. pmc Methylation-mediated transcriptional repression of microRNAs during cervical carcinogenesis
    Saskia M Wilting
    Department of Pathology, VU University Medical Center, Amsterdam, The Netherlands
    Epigenetics 8:220-8. 2013
    ..Increased hsa-miR-203 methylation was detectable in scrapes of women with high-grade CIN, indicating that methylated miRNAs may provide putative markers to assess the presence of (pre)cancerous lesions...
  25. ncbi request reprint Interplay between microRNAs and RNA-binding proteins determines developmental processes
    Martijn Kedde
    The Netherlands Cancer Institute, Division of Tumor Biology, Amsterdam, The Netherlands
    Cell Cycle 7:899-903. 2008
    ..In this perspective we discuss the interplay between RBPs and miRNAs in the context of germ cells and review current observations implicating RBPs in miRNA function...
  26. ncbi request reprint Unraveling human tumor suppressor pathways: a tale of the INK4A locus
    P Mathijs Voorhoeve
    Division of Tumor Biology, The Netherlands Cancer Institute, Amsterdam
    Cell Cycle 3:616-20. 2004
    ..The potential contribution of these tumor suppressors to the prevention of transformation of human cells can now begin to be unraveled by the recent emergence of novel RNA interference genetic tools...
  27. doi request reprint Functional genetic screens for enhancer elements in the human genome using CRISPR-Cas9
    Gozde Korkmaz
    Division of Biological Stress Response, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    Nat Biotechnol 34:192-8. 2016
    ..Moreover, we show that a genomic CRISPR-Cas9 tiling screen can precisely map functional domains within enhancer elements. Our approach expands the utility of CRISPR-Cas9 to elucidate the functions of the noncoding genome...
  28. pmc A versatile microsatellite instability reporter system in human cells
    Wouter Koole
    Department of Toxicogenetics, Leiden University Medical Center, Leiden 2333 ZC, The Netherlands and Division of Gene Regulation, The Netherlands Cancer Institute, Amsterdam 1066 CX, The Netherlands
    Nucleic Acids Res 41:e158. 2013
    ..Our data also provide proof of principle for the strategy of combining fluorescent reporters with next-generation sequencing technology to identify genetic factors in specific pathways. ..
  29. ncbi request reprint CDK-dependent stabilization of Cdc6: linking growth and stress signals to activation of DNA replication
    Anja M Duursma
    The Netherlands Cancer Institute, Amsterdam, The Netherlands
    Cell Cycle 4:1725-8. 2005
    ..New observations revealing that the initiation protein Cdc6 is stabilized by CDK2-dependent phosphorylation may give more insight in this process...
  30. doi request reprint MicroRNA regulation by RNA-binding proteins and its implications for cancer
    Marieke van Kouwenhove
    Division of Gene Regulation, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands
    Nat Rev Cancer 11:644-56. 2011
    ..In reviewing emerging concepts of the interplay between miRNAs and RNA-binding proteins, we highlight the implications of these complex layers of regulation in cancer initiation and progression...
  31. ncbi request reprint Immense promises for tiny molecules: uncovering miRNA functions
    Carlos le Sage
    Division of Tumor Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    Cell Cycle 5:1415-21. 2006
    ..Here we review the main approaches used to identify and validate miRNAs and describe ways to discover their functions. As potential effectors of many cellular pathways, mis-expression of miRNAs has been implicated in human cancer...
  32. pmc 3'UTR-mediated gene silencing of the Mixed Lineage Leukemia (MLL) gene
    Maria Gomez-Benito
    Division of Gene Regulation The Netherlands Cancer Institute, Amsterdam, The Netherlands
    PLoS ONE 6:e25449. 2011
    ..Altogether, our findings indicate that MLL translocations relieve oncogenic MLL-PG fusions from the repressive MLL 3'UTR, contributing to higher activity of these genes and leukaemia development...
  33. doi request reprint Identification of lethal microRNAs specific for head and neck cancer
    Marlon Lindenbergh-van der Plas
    Authors Affiliations Departments of Otolaryngology Head Neck Surgery, Epidemiology and Biostatistics, and Pathology, VU University Medical Center Department of Mathematics, VU University Division of Gene Regulation, The Netherlands Cancer Institute, Amsterdam and InteRNA Technologies BV, Utrecht, The Netherlands
    Clin Cancer Res 19:5647-57. 2013
    ..We identified by a functional genetic screen microRNAs that are selectively lethal for head and neck cancer cells but not for normal cells. We further investigated the genes targeted by these microRNAs...
  34. pmc Tumorigenicity of the miR-17-92 cluster distilled
    Gijs van Haaften
    Division of Gene Regulation, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands
    Genes Dev 24:1-4. 2010
    ..2806-2811) and Olive and colleagues (pp. 2839-2849) dissected the miR-17-92 cluster to its individual miRNA components and identified their relative contributions to oncogenic transformation in mouse model systems...
  35. doi request reprint A PASport to cellular proliferation
    Eitan Zlotorynski
    The Netherlands Cancer Institute, Division of Gene Regulation, Plesmanlaan 121, 1066CX, Amsterdam, The Netherlands
    Cell 134:208-10. 2008
    ..A recent report in Science (Sandberg et al., 2008) suggests that alternative polyadenylation is connected to microRNA-mediated regulation of gene expression as part of a global program for cellular proliferation...
  36. ncbi request reprint Ras interference as cancer therapy
    Anja M Duursma
    Division of Tumor Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Semin Cancer Biol 13:267-73. 2003
    ..The therapeutic potential of a RNAi-mediating vector was recently demonstrated by the stable suppression of oncogenic K-Ras in tumor cells...
  37. ncbi request reprint Diverse ways to control p27Kip1 function: miRNAs come into play
    Carlos le Sage
    Division of Tumor Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    Cell Cycle 6:2742-9. 2007
    ..Additionally, we focus on the different ways in which p27 is managed, argue how miRNAs could be involved in the regulation of p27 in both normal and malignant conditions, and discuss possible use of this knowledge for cancer therapy...
  38. ncbi request reprint Convergence of mitogenic and DNA damage signaling in the G1 phase of the cell cycle
    Reuven Agami
    Division of Molecular Carcinogenesis and Center for Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands
    Cancer Lett 177:111-8. 2002
    ..This review will highlight and compare aspects of the G1 arrest in response to stress generated either by lack of mitogens or damage to DNA...
  39. doi request reprint Tumour-specific proline vulnerability uncovered by differential ribosome codon reading
    Fabricio Loayza-Puch
    Division of Biological Stress Response, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Nature 530:490-4. 2016
    ..Thus, diricore has the potential to reveal unknown amino acid deficiencies, vulnerabilities that can be used to target key metabolic pathways for cancer treatment. ..
  40. pmc Removal of AU bias from microarray mRNA expression data enhances computational identification of active microRNAs
    Ran Elkon
    Division of Gene Regulation, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    PLoS Comput Biol 4:e1000189. 2008
    ..Our results substantiate that, after removal of AU biases, mRNA expression profiles contain ample information which allows in silico detection of miRs that are active in physiological conditions...
  41. ncbi request reprint Classifying microRNAs in cancer: the good, the bad and the ugly
    P Mathijs Voorhoeve
    Division of Tumor Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    Biochim Biophys Acta 1775:274-82. 2007
    ..Hereunder we will discuss the possible ways in which miRNAs can be relevant to cancer biology, and possible experimental strategies for elucidating the mechanisms involved...
  42. pmc p53-Dependent regulation of Cdc6 protein stability controls cellular proliferation
    Anja Duursma
    Division of Tumor Biology, The Netherlands Cancer Institute, Amsterdam
    Mol Cell Biol 25:6937-47. 2005
    ..Collectively, our data suggest that initiation of DNA replication is regulated by p53 through Cdc6 protein stability...
  43. pmc Major role for mRNA stability in shaping the kinetics of gene induction
    Ran Elkon
    Division of Gene Regulation, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    BMC Genomics 11:259. 2010
    ..In particular, kinetic waves in transcriptional responses are usually interpreted as resulting from sequential activation of transcription factors...
  44. ncbi request reprint The tumor-suppressive functions of the human INK4A locus
    P Mathijs Voorhoeve
    Division of Tumor Biology, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Cancer Cell 4:311-9. 2003
    ..Our results delineate the functions of the human INK4A genes in normal and tumorigenic growth...
  45. doi request reprint The miRNA-192/194 cluster regulates the Period gene family and the circadian clock
    Remco Nagel
    Division of Gene Regulation, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    FEBS J 276:5447-55. 2009
    ..In accordance, the exogenous expression of miR-192/194 leads to an altered circadian rhythm. Thus, our results have uncovered a new mechanism for the control of the circadian clock at the post-transcriptional level...
  46. doi request reprint Applying CRISPR-Cas9 tools to identify and characterize transcriptional enhancers
    Rui Lopes
    Division of Biological Stress Response, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    Nat Rev Mol Cell Biol 17:597-604. 2016
    ..Here, we discuss the application, current limitations and future development of CRISPR-Cas9 systems to identify and characterize enhancer elements in a high-throughput manner. ..
  47. ncbi request reprint A genetic screen implicates miRNA-372 and miRNA-373 as oncogenes in testicular germ cell tumors
    P Mathijs Voorhoeve
    Division of Tumour Biology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
    Cell 124:1169-81. 2006
    ..We provide evidence that these miRNAs are potential novel oncogenes participating in the development of human testicular germ cell tumors by numbing the p53 pathway, thus allowing tumorigenic growth in the presence of wild-type p53...
  48. pmc Ribosome profiling reveals features of normal and disease-associated mitochondrial translation
    Koos Rooijers
    1 Division of Gene Regulation, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands 2
    Nat Commun 4:2886. 2013
    ..Intriguingly, long RPFs are enriched in mutated mitochondria, suggesting they characterize stalled ribosomes. Our findings provide the first model for translation in wild-type and disease-triggering mitochondria. ..
  49. pmc eRNAs reach the heart of transcription
    Carlos A Melo
    1 Division of Gene Regulation, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands 2 Doctoral Programme in Biomedicine and Experimental Biology, Centre for Neuroscience and Cell Biology, Erasmus MC, Rotterdam University, The Netherlands
    Cell Res 23:1151-2. 2013
    ..Two recent studies published in Nature by Li et al. and Lam et al. highlight the importance of these newly characterized RNA molecules and their key role in controlling transcriptional programs. ..
  50. doi request reprint Transformation locked in a loop
    Jarno Drost
    Division of Gene Regulation, The Netherlands Cancer Institute, 1066 CX Amsterdam, The Netherlands
    Cell 139:654-6. 2009
    ..2009) now show that in certain cell types, a transient oncogenic signal is sufficient to induce neoplastic transformation and to maintain it through a positive feedback loop driven by the inflammatory cytokine interleukin-6...
  51. ncbi request reprint Knockdown stands up
    P Mathijs Voorhoeve
    Division of Tumor Biology and Center for Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands
    Trends Biotechnol 21:2-4. 2003
    ..Recently, two papers have shown that genes could be suppressed in vivo in mammals by RNAi, which has potential implications for both therapeutics and research...
  52. ncbi request reprint Stable suppression of tumorigenicity by virus-mediated RNA interference
    Thijn R Brummelkamp
    Division of Molecular Carcinogenesis, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands
    Cancer Cell 2:243-7. 2002
    ..These results indicate that viral delivery of small interfering RNAs can be used for tumor-specific gene therapy to reverse the oncogenic phenotype of cancer cells...
  53. pmc Survivin is required for a sustained spindle checkpoint arrest in response to lack of tension
    Susanne M A Lens
    Division of Molecular Biology, Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX Amsterdam, The Netherlands
    EMBO J 22:2934-47. 2003
    ..However, stable association of BubR1 to kinetochores and sustained checkpoint signalling in response to lack of tension crucially depend on survivin...
  54. doi request reprint The microRNAs miR-373 and miR-520c promote tumour invasion and metastasis
    Qihong Huang
    The Wistar Institute, 3601 Spruce Street, Philadelphia, PA 19104, USA
    Nat Cell Biol 10:202-10. 2008
    ..Taken together, our findings indicate that miRNAs are involved in tumour migration and invasion, and implicate miR-373 and miR-520c as metastasis-promoting miRNAs...
  55. pmc Inhibition of oncogenic transformation by mammalian Lin-9, a pRB-associated protein
    Sladjana Gagrica
    Institute for Molecular Biology and Tumor Research IMT, Philipps University Marburg, Marburg, Germany
    EMBO J 23:4627-38. 2004
    ..These data suggest that hLin-9 has tumor-suppressing activities and that the ability of hLin-9 to inhibit transformation is mediated through its association with pRB...
  56. pmc Specific inhibition of gene expression using a stably integrated, inducible small-interfering-RNA vector
    Marc van de Wetering
    Hubrecht Laboratory, Centre for Biomedical Genetics, Uppsalalaan 8, 3584 CT Utrecht, The Netherlands
    EMBO Rep 4:609-15. 2003
    ..The inhibition of two other genes using this vector system shows that it should be useful for the inducible knockdown of gene expression...
  57. pmc Homologous recombination and nonhomologous end-joining repair pathways regulate fragile site stability
    Michal Schwartz
    Department of Genetics, The Life Sciences Institute, The Hebrew University, Jerusalem, Israel
    Genes Dev 19:2715-26. 2005
    ..These findings suggest that DSBs are formed at common fragile sites as a result of replication perturbation. The repair of these breaks by both HR and NHEJ pathways is essential for chromosomal stability at these sites...
  58. ncbi request reprint shRNA-mediated RNA interference as a tool for genetic synthetic lethality screening in mouse embryo fibroblasts
    Yulia Einav
    Department of Biochemistry, The George S Wise Faculty of Life Sciences, Tel Aviv University, Ramat Aviv 69978, Israel
    FEBS Lett 579:199-202. 2005
    ..These findings open up the possibility of using in mammalian cells genome-wide shRNA libraries for genetic synthetic lethality screening at the multi-gene level...