Peter J M Van Haastert

Summary

Affiliation: University of Groningen
Country: The Netherlands

Publications

  1. pmc Distinct roles of PI(3,4,5)P3 during chemoattractant signaling in Dictyostelium: a quantitative in vivo analysis by inhibition of PI3-kinase
    Harriet M Loovers
    Department of Molecular Cell Biology, University of Groningen, 9751 NN Haren, The Netherlands
    Mol Biol Cell 17:1503-13. 2006
  2. pmc Coupled excitable Ras and F-actin activation mediates spontaneous pseudopod formation and directed cell movement.
    Peter J M Van Haastert
    Mol Biol Cell 28:922-934. 2017
  3. pmc A model for a correlated random walk based on the ordered extension of pseudopodia
    Peter J M Van Haastert
    Department of Cell Biochemistry, University of Groningen, Haren, The Netherlands
    PLoS Comput Biol 6:. 2010
  4. pmc Amoeboid cells use protrusions for walking, gliding and swimming
    Peter J M Van Haastert
    Department of Cell Biochemistry, University of Groningen, Groningen, The Netherlands
    PLoS ONE 6:e27532. 2011
  5. ncbi request reprint Chemotaxis: navigating by multiple signaling pathways
    Peter J M Van Haastert
    Department of Biology, University of Groningen, Kerklaan 30, NN Haren, Netherlands
    Sci STKE 2007:pe40. 2007
  6. pmc Essential role of PI3-kinase and phospholipase A2 in Dictyostelium discoideum chemotaxis
    Peter J M Van Haastert
    Department of Molecular Cell Biology, University of Groningen, 9751NN Haren, The Netherlands
    J Cell Biol 177:809-16. 2007
  7. pmc Biased random walk by stochastic fluctuations of chemoattractant-receptor interactions at the lower limit of detection
    Peter J M Van Haastert
    Department of Molecular Cell Biology, University of Groningen, Haren, The Netherlands
    Biophys J 93:1787-96. 2007
  8. pmc Food searching strategy of amoeboid cells by starvation induced run length extension
    Peter J M Van Haastert
    Department of Cell Biochemistry, University of Groningen, Haren, The Netherlands
    PLoS ONE 4:e6814. 2009
  9. doi request reprint Chemotaxis: insights from the extending pseudopod
    Peter J M Van Haastert
    Department of Cell Biochemistry, University of Groningen, Kerklaan 30, 9751NN Haren, The Netherlands
    J Cell Sci 123:3031-7. 2010
  10. pmc A stochastic model for chemotaxis based on the ordered extension of pseudopods
    Peter J M Van Haastert
    Department of Cell Biochemistry, University of Groningen, Groningen, The Netherlands
    Biophys J 99:3345-54. 2010

Collaborators

Detail Information

Publications61

  1. pmc Distinct roles of PI(3,4,5)P3 during chemoattractant signaling in Dictyostelium: a quantitative in vivo analysis by inhibition of PI3-kinase
    Harriet M Loovers
    Department of Molecular Cell Biology, University of Groningen, 9751 NN Haren, The Netherlands
    Mol Biol Cell 17:1503-13. 2006
    ....
  2. pmc Coupled excitable Ras and F-actin activation mediates spontaneous pseudopod formation and directed cell movement.
    Peter J M Van Haastert
    Mol Biol Cell 28:922-934. 2017
    ....
  3. pmc A model for a correlated random walk based on the ordered extension of pseudopodia
    Peter J M Van Haastert
    Department of Cell Biochemistry, University of Groningen, Haren, The Netherlands
    PLoS Comput Biol 6:. 2010
    ..Experiments confirm predictions of the model, showing reduced persistence in mutants that are defective in pseudopod splitting and in mutants with an irregular cell surface...
  4. pmc Amoeboid cells use protrusions for walking, gliding and swimming
    Peter J M Van Haastert
    Department of Cell Biochemistry, University of Groningen, Groningen, The Netherlands
    PLoS ONE 6:e27532. 2011
    ..Calculations suggest that these bumps provide sufficient drag force to mediate the observed forward swimming of the cell...
  5. ncbi request reprint Chemotaxis: navigating by multiple signaling pathways
    Peter J M Van Haastert
    Department of Biology, University of Groningen, Kerklaan 30, NN Haren, Netherlands
    Sci STKE 2007:pe40. 2007
    ..In addition, selective pseudopod growth and retraction are more important in directing cell movement than is the place where new pseudopodia are formed...
  6. pmc Essential role of PI3-kinase and phospholipase A2 in Dictyostelium discoideum chemotaxis
    Peter J M Van Haastert
    Department of Molecular Cell Biology, University of Groningen, 9751NN Haren, The Netherlands
    J Cell Biol 177:809-16. 2007
    ....
  7. pmc Biased random walk by stochastic fluctuations of chemoattractant-receptor interactions at the lower limit of detection
    Peter J M Van Haastert
    Department of Molecular Cell Biology, University of Groningen, Haren, The Netherlands
    Biophys J 93:1787-96. 2007
    ..Potential candidates for such second messengers are discussed...
  8. pmc Food searching strategy of amoeboid cells by starvation induced run length extension
    Peter J M Van Haastert
    Department of Cell Biochemistry, University of Groningen, Haren, The Netherlands
    PLoS ONE 4:e6814. 2009
    ..The SIRE walk explains very well the observed differences in search behavior between fed and starving organisms such as bumble-bees, flower bug, hoverfly and zooplankton...
  9. doi request reprint Chemotaxis: insights from the extending pseudopod
    Peter J M Van Haastert
    Department of Cell Biochemistry, University of Groningen, Kerklaan 30, 9751NN Haren, The Netherlands
    J Cell Sci 123:3031-7. 2010
    ..In this Commentary, I review the findings of these recent experiments, which together provide a new view of cell movement and chemotaxis...
  10. pmc A stochastic model for chemotaxis based on the ordered extension of pseudopods
    Peter J M Van Haastert
    Department of Cell Biochemistry, University of Groningen, Groningen, The Netherlands
    Biophys J 99:3345-54. 2010
    ..These results show that combining splitting and de novo pseudopods is a very effective way for cells to obtain very high sensitivity to stable gradient and still be responsive to changes in the direction of the gradient...
  11. doi request reprint How cells use pseudopods for persistent movement and navigation
    Peter J M Van Haastert
    Department of Cell Biochemistry, University of Groningen, Nijenborg 7, 9747AG Groningen, Netherlands
    Sci Signal 4:pe6. 2011
    ..Here, these data on pseudopod extensions are discussed in the context of a theoretical model for pseudopod splitting...
  12. ncbi request reprint RasGEF-containing proteins GbpC and GbpD have differential effects on cell polarity and chemotaxis in Dictyostelium
    Leonard Bosgraaf
    Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    J Cell Sci 118:1899-910. 2005
    ..GbpD induces the formation of substrate-attached pseudopodia, resulting in increased attachment and suppression of polarity...
  13. ncbi request reprint Sensitization of Dictyostelium chemotaxis by phosphoinositide-3-kinase-mediated self-organizing signalling patches
    Marten Postma
    Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    J Cell Sci 117:2925-35. 2004
    ..Thus, very low cAMP concentrations induce self-organizing PH(Crac)-GFP patches that serve as a spatial cue for pseudopod formation, which enhances the sensitivity and amplitude of chemotactic movement...
  14. pmc Activation of soluble guanylyl cyclase at the leading edge during Dictyostelium chemotaxis
    Douwe M Veltman
    Department of Biochemistry, University of Groningen, 9747 AG Groningen, The Netherlands
    Mol Biol Cell 16:976-83. 2005
    ..In a cAMP gradient, sGC-GFP localizes to the anterior cell cortex, suggesting that in chemotacting cells, sGC is activated at the leading edge of the cell...
  15. doi request reprint PI3-kinase signaling contributes to orientation in shallow gradients and enhances speed in steep chemoattractant gradients
    Leonard Bosgraaf
    Department of Molecular Cell Biology, University of Groningen, Kerklaan 30, 9751NN Haren, The Netherlands
    J Cell Sci 121:3589-97. 2008
    ..These results show that, although PI3K signaling is dispensable for chemotaxis, it gives the wild type an advantage over mutant cells...
  16. pmc A Rap/phosphatidylinositol 3-kinase pathway controls pseudopod formation [corrected]
    Arjan Kortholt
    Department of Molecular Cell Biology, University of Groningen, 9751 NN Haren, The Netherlands
    Mol Biol Cell 21:936-45. 2010
    ..As in Rap-regulated pseudopod formation in Dictyostelium, mammalian Rap and PI3K are essential for determining neuronal polarity, suggesting that the Rap/PI3K pathway is a conserved module regulating the establishment of cell polarity...
  17. pmc Multiple regulatory mechanisms for the Dictyostelium Roco protein GbpC
    Arjan Kortholt
    Department of Cell Biochemistry, University of Groningen, Nijenborgh 7, 9747 AG Groningen, The Netherlands
    J Biol Chem 287:2749-58. 2012
    ....
  18. pmc Dictyostelium chemotaxis: essential Ras activation and accessory signalling pathways for amplification
    Arjan Kortholt
    Department of Cell Biochemistry, University of Groningen, Nijenborgh 7, 9747 AG Groningen, The Netherlands
    EMBO Rep 12:1273-9. 2011
    ....
  19. pmc Chemoattractants and chemorepellents act by inducing opposite polarity in phospholipase C and PI3-kinase signaling
    Ineke Keizer-Gunnink
    Department of Molecular Cell Biology, University of Groningen, Haren, Netherlands
    J Cell Biol 177:579-85. 2007
    ..This leads to the accumulation of PI(3,4,5)P(3) at the rear of the cell, and chemotaxis occurs away from the source. We conclude that a PLC polarity switch controls the response to attractants and repellents...
  20. pmc Guanylyl cyclase protein and cGMP product independently control front and back of chemotaxing Dictyostelium cells
    Douwe M Veltman
    Department of Biology, University of Groningen, 9751 NN Haren, The Netherlands
    Mol Biol Cell 17:3921-9. 2006
    ..Thus cGMP suppresses pseudopod formation in the back of the cell, whereas the sGC protein refines pseudopod formation at the leading edge...
  21. pmc Four key signaling pathways mediating chemotaxis in Dictyostelium discoideum
    Douwe M Veltman
    Department of Molecular Cell Biology, University of Groningen, 9751 NN Haren, Netherlands
    J Cell Biol 180:747-53. 2008
    ..Cells that express only rear activity are polarized but do not exhibit chemotaxis, whereas cells with only front signaling are unpolarized but undergo chemotaxis...
  22. pmc Intramolecular activation mechanism of the Dictyostelium LRRK2 homolog Roco protein GbpC
    Wouter N van Egmond
    Department of Cell Biochemistry, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands
    J Biol Chem 283:30412-20. 2008
    ....
  23. ncbi request reprint Regulation of phagocytosis in Dictyostelium by the inositol 5-phosphatase OCRL homolog Dd5P4
    Harriet M Loovers
    Department of Molecular Cell Biology, University of Groningen, Kerklaan 30, 9751NN Haren, The Netherlands
    Traffic 8:618-28. 2007
    ..Growth on bacteria and axenic medium is largely restored, whereas the rate of phagocytosis of yeast cells is partly restored, indicating that human OCRL can functionally replace Dictyostelium Dd5P4...
  24. pmc Characterization of the Roco protein family in Dictyostelium discoideum
    Wouter N van Egmond
    Department of Cell Biochemistry, University of Groningen, Kerklaan 30, 9751 NN Haren, Netherlands
    Eukaryot Cell 9:751-61. 2010
    ..Together, our data show that the Dictyostelium Roco proteins serve a surprisingly diverse set of functions and highlight Roco4 as a key protein for proper stalk cell formation...
  25. doi request reprint Analysis of cell movement by simultaneous quantification of local membrane displacement and fluorescent intensities using Quimp2
    Leonard Bosgraaf
    University of Groningen, Cell Biochemistry Department, Haren, The Netherlands
    Cell Motil Cytoskeleton 66:156-65. 2009
    ..Quimp2 is used here to investigate the molecular mechanism of cell movement by correlating the dynamics of local membrane movement with the local concentration of myosin and F-actin...
  26. pmc Uniform cAMP stimulation of Dictyostelium cells induces localized patches of signal transduction and pseudopodia
    Marten Postma
    Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Mol Biol Cell 14:5019-27. 2003
    ....
  27. ncbi request reprint Characterization of the GbpD-activated Rap1 pathway regulating adhesion and cell polarity in Dictyostelium discoideum
    Arjan Kortholt
    Department of Molecular Cell Biology, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands
    J Biol Chem 281:23367-76. 2006
    ..Phg2, a serine/threonine-specific kinase, directly interacts with Rap1 via its Ras association domain...
  28. ncbi request reprint DdPDE4, a novel cAMP-specific phosphodiesterase at the surface of dictyostelium cells
    Sonya Bader
    Department of Molecular Cell Biology, University of Groningen, Kerklaan 30, 9751NN Haren, The Netherlands
    J Biol Chem 281:20018-26. 2006
    ..These results demonstrate that DdPDE4 is a unique membrane-bound phosphodiesterase with an extracellular catalytic domain regulating intercellular cAMP during multicellular development...
  29. pmc The phosducin-like protein PhLP1 is essential for G{beta}{gamma} dimer formation in Dictyostelium discoideum
    Jaco C Knol
    Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Mol Cell Biol 25:8393-400. 2005
    ..We propose that PhLP1 serves as a cochaperone assisting the assembly of Gbeta and Ggamma into a functional Gbetagamma complex. Thus, phosducin family proteins may fulfill hitherto unsuspected biosynthetic functions...
  30. pmc Phospholipase C regulation of phosphatidylinositol 3,4,5-trisphosphate-mediated chemotaxis
    Arjan Kortholt
    Department of Molecular Cell Biology, University of Groningen, 9751 NN Haren, The Netherlands
    Mol Biol Cell 18:4772-9. 2007
    ..These results indicate that local activation of PLC can control PTEN localization and suggest a novel mechanism to regulate the internal PI(3,4,5)P(3) gradient...
  31. pmc A novel cGMP signalling pathway mediating myosin phosphorylation and chemotaxis in Dictyostelium
    Leonard Bosgraaf
    Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    EMBO J 21:4560-70. 2002
    ..Thus, a novel cGMP signalling cascade is critical for chemotaxis in Dictyostelium, and plays a major role in myosin II regulation during this process...
  32. pmc Seven Dictyostelium discoideum phosphodiesterases degrade three pools of cAMP and cGMP
    Sonya Bader
    Department of Molecular Cell Biology, University of Groningen, Kerklaan 30, 9751NN, Haren, The Netherlands
    Biochem J 402:153-61. 2007
    ..The analysis shows that the combination of enzymes is tuned to keep the concentration and lifetime of the substrate within a functional range...
  33. pmc Chemotaxis: signalling modules join hands at front and tail
    Marten Postma
    Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    EMBO Rep 5:35-40. 2004
    ....
  34. pmc Dictyostelium Ric8 is a nonreceptor guanine exchange factor for heterotrimeric G proteins and is important for development and chemotaxis
    Rama Kataria
    Department of Cell Biochemistry, University of Groningen, 9747 AG Groningen, The Netherlands
    Proc Natl Acad Sci U S A 110:6424-9. 2013
    ....
  35. ncbi request reprint The role of cGMP and the rear of the cell in Dictyostelium chemotaxis and cell streaming
    Douwe M Veltman
    Department of Biology, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands
    J Cell Sci 121:120-7. 2008
    ..The results show that a quiescent rear of the cell increases the efficiency of directional movement and is essential to maintain stable cell-cell contacts...
  36. pmc Direct Interaction between TalinB and Rap1 is necessary for adhesion of Dictyostelium cells
    Katarzyna Plak
    Department of Cell Biochemistry, University of Groningen, Nijenborgh 7, Groningen, AG 9747, The Netherlands
    BMC Cell Biol 17:1. 2016
    ..In mammalian cells talin is crucial for adhesion and Rap1 was shown to be a key regulator of talin signalling...
  37. ncbi request reprint The regulation of myosin II in Dictyostelium
    Leonard Bosgraaf
    Department of Biology, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands
    Eur J Cell Biol 85:969-79. 2006
    ..Unlike conventional light chain kinases, this enzyme is not regulated by calcium but is activated by cGMP-induced phosphorylation via an upstream kinase and subsequent autophosphorylation...
  38. doi request reprint Ras activation and symmetry breaking during Dictyostelium chemotaxis
    Arjan Kortholt
    Department of Cell Biochemistry, University of Groningen, Nijenborgh 7, 9747 AG Groningen, The Netherlands
    J Cell Sci 126:4502-13. 2013
    ..The experiments show that activation, symmetry breaking and confinement of Ras during Dictyostelium chemotaxis uses different G-protein subunits and a multitude of Ras GEFs and GTPase-activating proteins (GAPs)...
  39. pmc GxcC connects Rap and Rac signaling during Dictyostelium development
    Katarzyna Plak
    Department of Cell Biochemistry, University of Groningen, Nijenborgh 7, Groningen, AG, 9747, The Netherlands
    BMC Cell Biol 14:6. 2013
    ..RapA is also important in late development, however so far little is known about the downstream effectors of RapA that play a role in this process...
  40. ncbi request reprint Chemotaxis: signalling the way forward
    Peter J M Van Haastert
    Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747AG Groningen, The Netherlands
    Nat Rev Mol Cell Biol 5:626-34. 2004
    ..Recent studies are beginning to identify an intricate network of signalling molecules that mediate these processes, and how these molecules become localized in the cell is now becoming clear...
  41. ncbi request reprint A model for cGMP signal transduction in Dictyostelium in perspective of 25 years of cGMP research
    Leonard Bosgraaf
    Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    J Muscle Res Cell Motil 23:781-91. 2002
    ..In this review we discuss these new findings in perspective of the previously obtained biochemical and functional data on cGMP in Dictyostelium...
  42. ncbi request reprint Analysis of signal transduction: formation of cAMP, cGMP, and Ins(1,4,5)P3 in vivo and in vitro
    Peter J M Van Haastert
    Department of Molecular Cell Biology, University of Groningen, Haren, The Netherlands
    Methods Mol Biol 346:369-92. 2006
    ..Finally, assays for the in vitro G protein-mediated activation of the effector enzymes adenylyl cyclase, guanylyl cyclase, and phospholipase C are described...
  43. ncbi request reprint Roc, a Ras/GTPase domain in complex proteins
    Leonard Bosgraaf
    Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Biochim Biophys Acta 1643:5-10. 2003
    ..Roc is always succeeded by a novel 300-400-amino-acid-long domain, termed COR. Proteins with Roc/COR are present in prokaryotes, Dictyostelium, plants and metazoa...
  44. ncbi request reprint Chemoattractant-stimulated calcium influx in Dictyostelium discoideum does not depend on cGMP
    Douwe M Veltman
    GBB Research School, Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Biochim Biophys Acta 1623:129-34. 2003
    ..The results reveal that the calcium influx stimulated by cAMP or folic acid is essentially identical in these strains. We conclude that cGMP is not involved in chemoattractant-stimulated calcium influx...
  45. pmc Phosducin-like proteins in Dictyostelium discoideum: implications for the phosducin family of proteins
    Mieke Blaauw
    Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    EMBO J 22:5047-57. 2003
    ..Phlp3 disruptants show no abnormal phenotype. These results establish a role for phosducin-like proteins in facilitating folding, localization or function of proteins, in addition to modulating G-protein signalling...
  46. doi request reprint A Gα-Stimulated RapGEF Is a Receptor-Proximal Regulator of Dictyostelium Chemotaxis
    Youtao Liu
    Department of Cell Biochemistry, University of Groningen, Nijenborgh 7, 9747 AG Groningen, The Netherlands
    Dev Cell 37:458-72. 2016
    ..Our observations demonstrate that GflB is an essential upstream regulator of chemoattractant-mediated cell polarity and cytoskeletal reorganization functioning to directly link Gα activation to monomeric G-protein signaling. ..
  47. pmc The small GTPases Ras and Rap1 bind to and control TORC2 activity
    Ankita Khanna
    Department of Cell Biochemistry, University of Groningen, Groningen, 9747AG, Netherlands
    Sci Rep 6:25823. 2016
    ..Dual Ras/Rap1 regulation of TORC2 may allow for integration of Ras and Rap1 signaling pathways in directed cell migration. ..
  48. pmc Navigation of chemotactic cells by parallel signaling to pseudopod persistence and orientation
    Leonard Bosgraaf
    Department of Cell Biochemistry, University of Groningen, Haren, The Netherlands
    PLoS ONE 4:e6842. 2009
    ..The coordinated regulation of pseudopod generation, orientation and persistence by multiple signaling pathways allows eukaryotic cells to detect extremely shallow gradients...
  49. doi request reprint A new set of small, extrachromosomal expression vectors for Dictyostelium discoideum
    Douwe M Veltman
    Cell Biochemistry, Department of Biology, University of Groningen, Haren, The Netherlands
    Plasmid 61:110-8. 2009
    ..In addition, two genes can be simultaneously expressed on one vector, which yields far more synchronous levels of expression than when expressing two genes on separate plasmids...
  50. pmc Identification and characterization of two unusual cGMP-stimulated phoshodiesterases in dictyostelium
    Leonard Bosgraaf
    Department of Biochemistry, University of Groningen, 9747 AG Groningen, The Netherlands
    Mol Biol Cell 13:3878-89. 2002
    ..The human cGMP-stimulated cAMP/cGMP phosphodiesterase has similar biochemical properties, but a completely different topology: hydrolysis takes place by a class I catalytic domain and GAF domains mediate cGMP activation...
  51. pmc Quimp3, an automated pseudopod-tracking algorithm
    Leonard Bosgraaf
    Department of Cell Biochemistry, University of Groningen, Haren, Netherlands
    Cell Adh Migr 4:46-55. 2010
    ..Here we describe the pseudopod tool and present the first data based on the analysis of approximately 1,000 pseudopodia extended by Dictyostelium cells in the absence of external cues...
  52. ncbi request reprint Unusual Guanylyl Cyclases and cGMP Signaling in Dictyostelium discoideum
    Douwe M Veltman
    Department of Biochemistry, University of Groningen, 9747 AG Groningen, The Netherlands
    Vitam Horm 69:95-115. 2004
    ..In this chapter we review the properties of the Dictyostelium guanylyl cyclases and discuss their role in the unusual cGMP pathway of Dictyostelium...
  53. pmc Rap1-dependent pathways coordinate cytokinesis in Dictyostelium
    Katarzyna Plak
    Department of Cell Biochemistry, University of Groningen, 9747 AG Groningen, Netherlands
    Mol Biol Cell 25:4195-204. 2014
    ..Importantly, mutated forms of Rap also affect cytokinesis in other organisms, suggesting a conserved role for Rap in cell division. ..
  54. pmc The ordered extension of pseudopodia by amoeboid cells in the absence of external cues
    Leonard Bosgraaf
    Department of Cell Biochemistry, University of Groningen, Haren, The Netherlands
    PLoS ONE 4:e5253. 2009
    ..The observed ordered extension of pseudopodia in the absence of external cues provides a fundamental insight into the coordinated movement of cells, and might form the basis for movement that is directed by internal or external cues...
  55. ncbi request reprint A diverse family of inositol 5-phosphatases playing a role in growth and development in Dictyostelium discoideum
    Harriet M Loovers
    Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    J Biol Chem 278:5652-8. 2003
    ..Inactivation of the gene coding for Dd5P4 leads to defects in growth and development. These defects are restored by the expression of the complete protein but not by the 5-phosphatase catalytic domain...
  56. doi request reprint Highlighting the role of Ras and Rap during Dictyostelium chemotaxis
    Arjan Kortholt
    Department of Molecular Cell Biology, University of Groningen, Kerklaan 30, 9751NN Haren, The Netherlands
    Cell Signal 20:1415-22. 2008
    ..Surprisingly, small G-proteins appear to play essential roles in all these pathways. This review will summarize the role of small G-proteins in Dictyostelium, particularly highlighting the function of the Ras subfamily in chemotaxis...
  57. pmc Roco kinase structures give insights into the mechanism of Parkinson disease-related leucine-rich-repeat kinase 2 mutations
    Bernd K Gilsbach
    Department of Cell Biochemistry, University of Groningen, 9747 AG, Groningen, The Netherlands
    Proc Natl Acad Sci U S A 109:10322-7. 2012
    ..Taken together, our data give important insight in the LRRK2 activation mechanism and, most importantly, explain the G2019S-related increase in LRRK2 kinase activity...
  58. ncbi request reprint Characterization of two unusual guanylyl cyclases from dictyostelium
    Jeroen Roelofs
    Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    J Biol Chem 277:9167-74. 2002
    ..Osmotic stress strongly stimulates sGC but has no effect on GCA activity. Finally, GCA is exclusively membrane-bound and is active mainly with Mg(2+), whereas sGC is predominantly soluble and more active with Mn(2+)...
  59. ncbi request reprint Production of the soluble human Fas ligand by Dictyostelium discoideum cultivated on a synthetic medium
    Yinghua Lu
    Department of Biochemistry, University of Groningen, Nijenborgh 4, NL 9747 AG Groningen, The Netherlands
    J Biotechnol 108:243-51. 2004
    ..discoideum in a bioreactor equipped with cell retention by microfiltration. At low space velocity very high cell densities of up to 2.4 x 10(8) ml(-1) and hFasL concentrations of up to 205 microgl(-1) were achieved...
  60. ncbi request reprint Deducing the origin of soluble adenylyl cyclase, a gene lost in multiple lineages
    Jeroen Roelofs
    GBB, Department of Biochemistry, University of Groningen, Nijenborgh 4, 9747 AG Groningen, The Netherlands
    Mol Biol Evol 19:2239-46. 2002
    ..We conclude that within the family of adenylyl cyclases, soluble AC was poorly fixed during evolution, whereas membrane-bound AC has expanded to form the subgroups of prevailing adenylyl and guanylyl cyclases...
  61. pmc The local cell curvature guides pseudopodia towards chemoattractants
    Peter J M Van Haastert
    Department of Cell Biochemistry, University of Groningen, Kerklaan 30, 9751 NN Haren, The Netherlands
    HFSP J 3:282-6. 2009
    ....