George W Lubega

Summary

Affiliation: Ministry of Health
Country: Uganda

Publications

  1. ncbi request reprint Immunization with a tubulin-rich preparation from Trypanosoma brucei confers broad protection against African trypanosomosis
    George W Lubega
    Molecular Biology Laboratory, Faculty of Veterinary Medicine, Makerere University, PO Box 7062, Kampala, Uganda
    Exp Parasitol 102:9-22. 2002
  2. ncbi request reprint Trypanosoma brucei: anti-tubulin antibodies specifically inhibit trypanosome growth in culture
    George W Lubega
    Molecular Biology Laboratory, Faculty of Veterinary Medicine, Makerere University, P O Box 7062, Kampala, Uganda
    Exp Parasitol 102:134-42. 2002
  3. doi request reprint Accordance and concordance of PCR and NASBA followed by oligochromatography for the molecular diagnosis of Trypanosoma brucei and Leishmania
    Claire M Mugasa
    Department of Veterinary Parasitology and Microbiology, Makerere University, Kampala, Uganda
    Trop Med Int Health 15:800-5. 2010
  4. pmc Nucleic acid sequence-based amplification with oligochromatography for detection of Trypanosoma brucei in clinical samples
    Claire M Mugasa
    Department of Veterinary Parasitology and Microbiology, Faculty of Veterinary Medicine, Makerere University, Kampala, Uganda
    J Clin Microbiol 47:630-5. 2009
  5. pmc Phase II evaluation of sensitivity and specificity of PCR and NASBA followed by oligochromatography for diagnosis of human African trypanosomiasis in clinical samples from D.R. Congo and Uganda
    Enock Matovu
    Department of Veterinary Parasitology and Microbiology, Makerere University, Kampala, Uganda
    PLoS Negl Trop Dis 4:e737. 2010
  6. pmc Effect of adjuvants on the humoral immune response to congopain in mice and cattle
    John Kateregga
    College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, Box 7062, Kampala, Uganda
    BMC Vet Res 8:63. 2012
  7. ncbi request reprint Classical ligands bind tubulin of trypanosomes and inhibit their growth in vitro
    Donasian O K Ochola
    Molecular Biology Laboratory, Faculty of Veterinary Medicine, Makerere University, Kampala, Uganda
    J Parasitol 88:600-4. 2002
  8. ncbi request reprint Classical ligands interact with native and recombinant tubulin from Onchocerca volvulus with similar rank order of magnitude
    Eddie M Wampande
    Department of Parasitology and Microbiology, Faculty of Veterinary Medicine, Makerere University P O Box 7062, Kampala, Uganda
    Protein Expr Purif 55:236-45. 2007
  9. doi request reprint Detection of Trypanosoma brucei parasites in blood samples using real-time nucleic acid sequence-based amplification
    Claire M Mugasa
    Faculty of Veterinary Medicine, Department of Veterinary Parasitology and Microbiology, Makerere University, Kampala, Uganda
    Diagn Microbiol Infect Dis 61:440-5. 2008
  10. doi request reprint Theileria parva genetic diversity and haemoparasite prevalence in cattle and wildlife in and around Lake Mburo National Park in Uganda
    Chris A L Oura
    Department of Microbiology and Parasitology, Faculty of Vet Medicine, University of Makerere, P O Box 7062, Kampala, Uganda
    Parasitol Res 108:1365-74. 2011

Collaborators

Detail Information

Publications12

  1. ncbi request reprint Immunization with a tubulin-rich preparation from Trypanosoma brucei confers broad protection against African trypanosomosis
    George W Lubega
    Molecular Biology Laboratory, Faculty of Veterinary Medicine, Makerere University, PO Box 7062, Kampala, Uganda
    Exp Parasitol 102:9-22. 2002
    ..These data suggest that tubulin is the relevant immunogen in the preparation used and could therefore be a promising target for the development of a parasite-specific, broad spectrum vaccine...
  2. ncbi request reprint Trypanosoma brucei: anti-tubulin antibodies specifically inhibit trypanosome growth in culture
    George W Lubega
    Molecular Biology Laboratory, Faculty of Veterinary Medicine, Makerere University, P O Box 7062, Kampala, Uganda
    Exp Parasitol 102:134-42. 2002
    ..This suggests that the variant surface glycoproteins (VSG) played no part in the generation of these inhibitory antibodies...
  3. doi request reprint Accordance and concordance of PCR and NASBA followed by oligochromatography for the molecular diagnosis of Trypanosoma brucei and Leishmania
    Claire M Mugasa
    Department of Veterinary Parasitology and Microbiology, Makerere University, Kampala, Uganda
    Trop Med Int Health 15:800-5. 2010
    ..To evaluate the repeatability and reproducibility of four simplified molecular assays for the diagnosis of Trypanosoma brucei spp. or Leishmania ssp. in a multicentre ring trial with seven participating laboratories...
  4. pmc Nucleic acid sequence-based amplification with oligochromatography for detection of Trypanosoma brucei in clinical samples
    Claire M Mugasa
    Department of Veterinary Parasitology and Microbiology, Faculty of Veterinary Medicine, Makerere University, Kampala, Uganda
    J Clin Microbiol 47:630-5. 2009
    ..brucei NASBA-OC test developed in this study can be employed in field laboratories, because it does not require a thermocycler; a simple heat block or a water bath maintained at two different temperatures is sufficient for amplification...
  5. pmc Phase II evaluation of sensitivity and specificity of PCR and NASBA followed by oligochromatography for diagnosis of human African trypanosomiasis in clinical samples from D.R. Congo and Uganda
    Enock Matovu
    Department of Veterinary Parasitology and Microbiology, Makerere University, Kampala, Uganda
    PLoS Negl Trop Dis 4:e737. 2010
    ..In this study we evaluate the sensitivity and specificity of the PCR-OC and NASBA-OC for diagnosis of Trypanosoma brucei gambiense and Trypanosoma brucei rhodesiense human African trypanosomiasis (HAT)...
  6. pmc Effect of adjuvants on the humoral immune response to congopain in mice and cattle
    John Kateregga
    College of Veterinary Medicine, Animal Resources and Biosecurity, Makerere University, Box 7062, Kampala, Uganda
    BMC Vet Res 8:63. 2012
    ....
  7. ncbi request reprint Classical ligands bind tubulin of trypanosomes and inhibit their growth in vitro
    Donasian O K Ochola
    Molecular Biology Laboratory, Faculty of Veterinary Medicine, Makerere University, Kampala, Uganda
    J Parasitol 88:600-4. 2002
    ..Trypanosome tubulin is, therefore, a reasonable target against which novel drugs can be developed to control trypanosomiasis...
  8. ncbi request reprint Classical ligands interact with native and recombinant tubulin from Onchocerca volvulus with similar rank order of magnitude
    Eddie M Wampande
    Department of Parasitology and Microbiology, Faculty of Veterinary Medicine, Makerere University P O Box 7062, Kampala, Uganda
    Protein Expr Purif 55:236-45. 2007
    ..These data suggest that recombinant tubulin can be used to develop a molecular screen for novel anti-tubulin ligands to develop into drugs against onchocerciasis...
  9. doi request reprint Detection of Trypanosoma brucei parasites in blood samples using real-time nucleic acid sequence-based amplification
    Claire M Mugasa
    Faculty of Veterinary Medicine, Department of Veterinary Parasitology and Microbiology, Makerere University, Kampala, Uganda
    Diagn Microbiol Infect Dis 61:440-5. 2008
    ..The assay detected 10 parasites/mL using cultured parasites as well as spiked blood. A sensitive assay such as the one developed in this study may become an alternative tool to confirm diagnosis of human African trypanosomiasis...
  10. doi request reprint Theileria parva genetic diversity and haemoparasite prevalence in cattle and wildlife in and around Lake Mburo National Park in Uganda
    Chris A L Oura
    Department of Microbiology and Parasitology, Faculty of Vet Medicine, University of Makerere, P O Box 7062, Kampala, Uganda
    Parasitol Res 108:1365-74. 2011
    ..parva genotypes to the cattle population...
  11. ncbi request reprint Detection of mutant P2 adenosine transporter (TbAT1) gene in Trypanosoma brucei gambiense isolates from northwest Uganda using allele-specific polymerase chain reaction
    Barbara Nerima
    National Livestock Health Research Institute, Tororo, Uganda
    Trop Med Int Health 12:1361-8. 2007
    ..To assess the application of allele-specific PCR (AS-PCR) as a fast, cheap and reliable method for detecting mutant TbAT1 associated with melarsoprol relapse in Trypanosoma brucei gambiense isolates from northwest Uganda...
  12. pmc Trypanosoma brucei prenylated-protein carboxyl methyltransferase prefers farnesylated substrates
    Frederick S Buckner
    Department of Medicine, University of Washington, Seattle, WA 98195, USA
    Biochem J 367:809-16. 2002
    ..brucei PPMT acts on farnesylated proteins in the cell. Cells of the T. brucei bloodstream form show higher sensitivity to AFC and AGGC (EC(50)=70-80 microM) compared with mouse 3T3 cells (EC(50)>150 microM)...