S R Wilkinson

Summary

Affiliation: London School of Hygiene and Tropical Medicine
Country: UK

Publications

  1. ncbi request reprint Distinct mitochondrial and cytosolic enzymes mediate trypanothione-dependent peroxide metabolism in Trypanosoma cruzi
    S R Wilkinson
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT United Kingdom
    J Biol Chem 275:8220-5. 2000
  2. ncbi request reprint Functional characterisation of the iron superoxide dismutase gene repertoire in Trypanosoma brucei
    Shane R Wilkinson
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK
    Free Radic Biol Med 40:198-209. 2006
  3. pmc Vitamin C biosynthesis in trypanosomes: a role for the glycosome
    Shane R Wilkinson
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom
    Proc Natl Acad Sci U S A 102:11645-50. 2005
  4. ncbi request reprint RNA interference identifies two hydroperoxide metabolizing enzymes that are essential to the bloodstream form of the african trypanosome
    Shane R Wilkinson
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom
    J Biol Chem 278:31640-6. 2003
  5. pmc Trypanosoma cruzi expresses a plant-like ascorbate-dependent hemoperoxidase localized to the endoplasmic reticulum
    Shane R Wilkinson
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK
    Proc Natl Acad Sci U S A 99:13453-8. 2002
  6. pmc TcGPXII, a glutathione-dependent Trypanosoma cruzi peroxidase with substrate specificity restricted to fatty acid and phospholipid hydroperoxides, is localized to the endoplasmic reticulum
    Shane R Wilkinson
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    Biochem J 364:787-94. 2002
  7. ncbi request reprint The Trypanosoma cruzi enzyme TcGPXI is a glycosomal peroxidase and can be linked to trypanothione reduction by glutathione or tryparedoxin
    Shane R Wilkinson
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom
    J Biol Chem 277:17062-71. 2002
  8. pmc Biochemical characterization of a trypanosome enzyme with glutathione-dependent peroxidase activity
    S R Wilkinson
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT, UK
    Biochem J 352:755-61. 2000
  9. doi request reprint Insights into the redox biology of Trypanosoma cruzi: Trypanothione metabolism and oxidant detoxification
    Florencia Irigoín
    Departmento de Histología y Embriología, Facultad de Medicina, Universidad de la Republica, Uruguay
    Free Radic Biol Med 45:733-42. 2008
  10. pmc Peroxiredoxins play a major role in protecting Trypanosoma cruzi against macrophage- and endogenously-derived peroxynitrite
    Lucia Piacenza
    Departamento de Bioquímica and Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Biochem J 410:359-68. 2008

Collaborators

Detail Information

Publications18

  1. ncbi request reprint Distinct mitochondrial and cytosolic enzymes mediate trypanothione-dependent peroxide metabolism in Trypanosoma cruzi
    S R Wilkinson
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT United Kingdom
    J Biol Chem 275:8220-5. 2000
    ..The parasite-specific features of T. cruzi cytoplasmic peroxiredoxin and T. cruzi mitochondrial peroxiredoxin may be exploitable in terms of drug development...
  2. ncbi request reprint Functional characterisation of the iron superoxide dismutase gene repertoire in Trypanosoma brucei
    Shane R Wilkinson
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK
    Free Radic Biol Med 40:198-209. 2006
    ..These experiments therefore identify essential components of the superoxide metabolising arm of the T. brucei oxidative defence system and validate these enzymes as parasite-specific targets for drug design...
  3. pmc Vitamin C biosynthesis in trypanosomes: a role for the glycosome
    Shane R Wilkinson
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom
    Proc Natl Acad Sci U S A 102:11645-50. 2005
    ..It is implicit, therefore, that bloodstream-form trypanosomes also possess a capacity for ascorbate transport...
  4. ncbi request reprint RNA interference identifies two hydroperoxide metabolizing enzymes that are essential to the bloodstream form of the african trypanosome
    Shane R Wilkinson
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom
    J Biol Chem 278:31640-6. 2003
    ..These experiments therefore identify redox pathways that are essential for oxidative defense in T. brucei and validate the corresponding peroxidases as targets for drug design...
  5. pmc Trypanosoma cruzi expresses a plant-like ascorbate-dependent hemoperoxidase localized to the endoplasmic reticulum
    Shane R Wilkinson
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK
    Proc Natl Acad Sci U S A 99:13453-8. 2002
    ..These findings identify an arm of the oxidative defense system of this medically important parasite. The absence of this redox pathway in the human host may be therapeutically exploitable...
  6. pmc TcGPXII, a glutathione-dependent Trypanosoma cruzi peroxidase with substrate specificity restricted to fatty acid and phospholipid hydroperoxides, is localized to the endoplasmic reticulum
    Shane R Wilkinson
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    Biochem J 364:787-94. 2002
    ..cruzi oxidative defence system by protecting newly synthesized lipids from peroxidation...
  7. ncbi request reprint The Trypanosoma cruzi enzyme TcGPXI is a glycosomal peroxidase and can be linked to trypanothione reduction by glutathione or tryparedoxin
    Shane R Wilkinson
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom
    J Biol Chem 277:17062-71. 2002
    ..The ability of TcGPXI to use alternative electron donors may reflect their availability at the corresponding subcellular sites...
  8. pmc Biochemical characterization of a trypanosome enzyme with glutathione-dependent peroxidase activity
    S R Wilkinson
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT, UK
    Biochem J 352:755-61. 2000
    ..With lower-affinity substrates such as t-butyl hydroperoxide, the rate-limiting step is the reduction of the oxidant. The data presented here identify a new arm of the T. cruzi oxidative defence system...
  9. doi request reprint Insights into the redox biology of Trypanosoma cruzi: Trypanothione metabolism and oxidant detoxification
    Florencia Irigoín
    Departmento de Histología y Embriología, Facultad de Medicina, Universidad de la Republica, Uruguay
    Free Radic Biol Med 45:733-42. 2008
    ..Together, the evidence suggests a central role of the trypanothione-dependent antioxidant systems in the infection process...
  10. pmc Peroxiredoxins play a major role in protecting Trypanosoma cruzi against macrophage- and endogenously-derived peroxynitrite
    Lucia Piacenza
    Departamento de Bioquímica and Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Biochem J 410:359-68. 2008
    ..Together with recent reports of up-regulation of antioxidant enzymes during metacyclogenesis, our results identify components of the antioxidant enzyme network of T. cruzi as virulence factors of emerging importance...
  11. pmc Validation of spermidine synthase as a drug target in African trypanosomes
    Martin C Taylor
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK
    Biochem J 409:563-9. 2008
    ..brucei cannot scavenge sufficient spermidine from their environment to meet growth requirements...
  12. pmc The terminal step in vitamin C biosynthesis in Trypanosoma cruzi is mediated by a FMN-dependent galactonolactone oxidase
    Flora J Logan
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    Biochem J 407:419-26. 2007
    ..The conserved lysine residue within this motif (Lys-450) is not required for cofactor binding, but its replacement by glycine renders the protein completely inactive...
  13. pmc Mitochondrial superoxide radicals mediate programmed cell death in Trypanosoma cruzi: cytoprotective action of mitochondrial iron superoxide dismutase overexpression
    Lucia Piacenza
    Departamento de Bioquimica, Center for Free Radical and Biomedical Research, Facultad de Medicina, Universidad de la Republica, Montevideo, Uruguay
    Biochem J 403:323-34. 2007
    ....
  14. pmc Deletion of the Trypanosoma brucei superoxide dismutase gene sodb1 increases sensitivity to nifurtimox and benznidazole
    S Radhika Prathalingham
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom
    Antimicrob Agents Chemother 51:755-8. 2007
    ..Here, we identify a superoxide dismutase required to protect Trypanosoma brucei from drug-generated superoxide...
  15. ncbi request reprint The amino terminal domain of a novel WD repeat protein from Trypanosoma cruzi contains a non-canonical mitochondrial targeting signal
    Elizabeth V Bromley
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, Keppel Street, London WC1E 7HT, UK
    Int J Parasitol 34:63-71. 2004
    ..Interestingly this region lacks a typical amino terminal presequence suggesting that mitochondrial import is mediated by an alternative targeting signal...
  16. pmc Functional mapping of a trypanosome centromere by chromosome fragmentation identifies a 16-kb GC-rich transcriptional "strand-switch" domain as a major feature
    Samson O Obado
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, United Kingdom
    Genome Res 15:36-43. 2005
    ..This unusual type of organization may represent a paradigm for centromeres in T. cruzi and other primitive eukaryotes...
  17. pmc A mechanism for cross-resistance to nifurtimox and benznidazole in trypanosomes
    Shane R Wilkinson
    School of Biological and Chemical Sciences, Queen Mary University of London, London E1 4NS, United Kingdom
    Proc Natl Acad Sci U S A 105:5022-7. 2008
    ..This potential for drug resistance by a simple mechanism has important implications, because nifurtimox is currently undergoing phase III clinical trials against African trypanosomiasis...
  18. ncbi request reprint The role of glutathione peroxidases in trypanosomatids
    Shane R Wilkinson
    Department of Infectious and Tropical Diseases, London School of Hygiene and Tropical Medicine, London WC1E 7HT, UK
    Biol Chem 384:517-25. 2003
    ....