Richard McCulloch

Summary

Country: UK

Publications

  1. pmc Does DNA replication direct locus-specific recombination during host immune evasion by antigenic variation in the African trypanosome?
    Rebecca Devlin
    The Wellcome Trust Centre for Molecular Parasitology, Institute of Infection, Immunity and Inflammation, University of Glasgow, Sir Graeme Davis Building, 120 University Place, Glasgow, G12 8TA, UK
    Curr Genet . 2016
  2. pmc Trypanosoma brucei BRCA2 acts in a life cycle-specific genome stability process and dictates BRC repeat number-dependent RAD51 subnuclear dynamics
    Anna Trenaman
    The Wellcome Trust Centre for Molecular Parasitology, College of Medical Veterinary and Life Sciences, Institute of Infection, Immunity and Inflammation, University of Glasgow, Sir Graeme Davies Building, 120 University Place, Glasgow G12 8TA, UK
    Nucleic Acids Res 41:943-60. 2013
  3. pmc Interactions among Trypanosoma brucei RAD51 paralogues in DNA repair and antigenic variation
    Rachel Dobson
    College of Medical Veterinary and Life Sciences, University of Glasgow, Institute of Infection, Immunity and Inflammation, The Wellcome Trust Centre for Molecular Parasitology, Sir Graeme Davis Building, 120 University Place, Glasgow G128TA, UK
    Mol Microbiol 81:434-56. 2011
  4. pmc Ku heterodimer-independent end joining in Trypanosoma brucei cell extracts relies upon sequence microhomology
    Peter Burton
    The Wellcome Centre for Molecular Parasitology, University of Glasgow, Glasgow Biomedical Research Centre, Glasgow, Scotland, UK
    Eukaryot Cell 6:1773-81. 2007
  5. ncbi request reprint Trypanosoma brucei DMC1 does not act in DNA recombination, repair or antigenic variation in bloodstream stage cells
    Chris Proudfoot
    The Wellcome Centre for Molecular Parasitology, University of Glasgow, Anderson College, 56 Dumbarton Road, Glasgow G11 6NU, UK
    Mol Biochem Parasitol 145:245-53. 2006
  6. pmc Distinct roles for two RAD51-related genes in Trypanosoma brucei antigenic variation
    Chris Proudfoot
    The Wellcome Centre for Molecular Parasitology, University of Glasgow, Anderson College, 56 Dumbarton Road, Glasgow, G11 6NU, UK
    Nucleic Acids Res 33:6906-19. 2005
  7. pmc Trypanosoma brucei BRCA2 acts in antigenic variation and has undergone a recent expansion in BRC repeat number that is important during homologous recombination
    Claire L Hartley
    The Wellcome Centre for Molecular Parasitology and Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow Biomedical Research Centre, 120 University Place, Glasgow G12 8TA, UK
    Mol Microbiol 68:1237-51. 2008
  8. doi request reprint What has DNA sequencing revealed about the VSG expression sites of African trypanosomes?
    Richard McCulloch
    University of Glasgow, Glasgow Biomedical Research Centre, UK
    Trends Parasitol 25:359-63. 2009
  9. pmc Genome-wide analysis reveals extensive functional interaction between DNA replication initiation and transcription in the genome of Trypanosoma brucei
    Calvin Tiengwe
    The Wellcome Trust Centre for Molecular Parasitology, College of Medical, Veterinary and Life Sciences, Institute of Infection, Immunity and Inflammation, University of Glasgow, Sir Graeme Davies Building, 120 University Place, Glasgow G12 8TA, UK
    Cell Rep 2:185-97. 2012
  10. pmc Identification of ORC1/CDC6-interacting factors in Trypanosoma brucei reveals critical features of origin recognition complex architecture
    Calvin Tiengwe
    The Wellcome Trust Centre for Molecular Parasitology, Institute of Infection, Immunity and Inflammation, University of Glasgow, Glasgow, United Kingdom
    PLoS ONE 7:e32674. 2012

Collaborators

  • J David Barry
  • Lucio Marcello
  • Stephen D Bell
  • David Horn
  • Keith Gull
  • Catarina Gadelha
  • Tansy C Hammarton
  • Liam J Morrison
  • Calvin Tiengwe
  • Chris Proudfoot
  • Rebecca Devlin
  • Catarina A Marques
  • Anna Trenaman
  • Peter Burton
  • Helen Farr
  • Rachel Dobson
  • Glynn R Forsythe
  • Claire L Hartley
  • Rebecca L Barnes
  • Joanna S Bell
  • Colin Conway
  • Marko Prorocic
  • Claire Hartley
  • Volodymyr Nechyporuk-Zloy
  • Moniek van den Hoek
  • Carlos R Machado
  • Danielle G Passos-Silva
  • Michal Swiderski
  • Richard Burchmore
  • Nicholas Dickens
  • Diane Vaughan
  • Steven Kelly
  • Christopher Stockdale
  • Craig Lapsley
  • Jonathan Wilkes
  • David J McBride
  • Jonathan M Wilkes
  • Timothy I Harvey
  • Anne Marie Sims

Detail Information

Publications17

  1. pmc Does DNA replication direct locus-specific recombination during host immune evasion by antigenic variation in the African trypanosome?
    Rebecca Devlin
    The Wellcome Trust Centre for Molecular Parasitology, Institute of Infection, Immunity and Inflammation, University of Glasgow, Sir Graeme Davis Building, 120 University Place, Glasgow, G12 8TA, UK
    Curr Genet . 2016
    ..Here, we discuss three emerging models for VSG switching initiation and ask how these compare with processes for adaptive genome change found in other organisms...
  2. pmc Trypanosoma brucei BRCA2 acts in a life cycle-specific genome stability process and dictates BRC repeat number-dependent RAD51 subnuclear dynamics
    Anna Trenaman
    The Wellcome Trust Centre for Molecular Parasitology, College of Medical Veterinary and Life Sciences, Institute of Infection, Immunity and Inflammation, University of Glasgow, Sir Graeme Davies Building, 120 University Place, Glasgow G12 8TA, UK
    Nucleic Acids Res 41:943-60. 2013
    ..brucei, and this function has necessitated the evolution of extensive RAD51 interaction via the BRC repeats, allowing re-localization of the recombinase to general genome damage when needed...
  3. pmc Interactions among Trypanosoma brucei RAD51 paralogues in DNA repair and antigenic variation
    Rachel Dobson
    College of Medical Veterinary and Life Sciences, University of Glasgow, Institute of Infection, Immunity and Inflammation, The Wellcome Trust Centre for Molecular Parasitology, Sir Graeme Davis Building, 120 University Place, Glasgow G128TA, UK
    Mol Microbiol 81:434-56. 2011
    ..brucei is unusually large among microbial eukaryotes and that one member of the protein family corresponds with a key, conserved eukaryotic Rad51 paralogue...
  4. pmc Ku heterodimer-independent end joining in Trypanosoma brucei cell extracts relies upon sequence microhomology
    Peter Burton
    The Wellcome Centre for Molecular Parasitology, University of Glasgow, Glasgow Biomedical Research Centre, Glasgow, Scotland, UK
    Eukaryot Cell 6:1773-81. 2007
    ..brucei, in common with other kinetoplastids, does not encode recognizable homologues of DNA ligase IV or XRCC4, suggesting that NHEJ is either absent or mechanistically diverged in these pathogens...
  5. ncbi request reprint Trypanosoma brucei DMC1 does not act in DNA recombination, repair or antigenic variation in bloodstream stage cells
    Chris Proudfoot
    The Wellcome Centre for Molecular Parasitology, University of Glasgow, Anderson College, 56 Dumbarton Road, Glasgow G11 6NU, UK
    Mol Biochem Parasitol 145:245-53. 2006
    ..DMC1 mutation does not, however, result in detectable alterations in DNA repair, recombination or antigenic variation efficiency in this life cycle stage...
  6. pmc Distinct roles for two RAD51-related genes in Trypanosoma brucei antigenic variation
    Chris Proudfoot
    The Wellcome Centre for Molecular Parasitology, University of Glasgow, Anderson College, 56 Dumbarton Road, Glasgow, G11 6NU, UK
    Nucleic Acids Res 33:6906-19. 2005
    ..brucei have assumed specialized functions in homologous recombination, analogous to related proteins in metazoan eukaryotes...
  7. pmc Trypanosoma brucei BRCA2 acts in antigenic variation and has undergone a recent expansion in BRC repeat number that is important during homologous recombination
    Claire L Hartley
    The Wellcome Centre for Molecular Parasitology and Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow Biomedical Research Centre, 120 University Place, Glasgow G12 8TA, UK
    Mol Microbiol 68:1237-51. 2008
    ..brucei homologous recombination and RAD51 localization. Remarkably, however, this appears not to be a major determinant of the activation of at least some VSG genes...
  8. doi request reprint What has DNA sequencing revealed about the VSG expression sites of African trypanosomes?
    Richard McCulloch
    University of Glasgow, Glasgow Biomedical Research Centre, UK
    Trends Parasitol 25:359-63. 2009
    ..This gap in our knowledge has now been bridged by two new studies, which we discuss here, asking what has been revealed about the biological significance of BES multiplicity and ESAG function and evolution...
  9. pmc Genome-wide analysis reveals extensive functional interaction between DNA replication initiation and transcription in the genome of Trypanosoma brucei
    Calvin Tiengwe
    The Wellcome Trust Centre for Molecular Parasitology, College of Medical, Veterinary and Life Sciences, Institute of Infection, Immunity and Inflammation, University of Glasgow, Sir Graeme Davies Building, 120 University Place, Glasgow G12 8TA, UK
    Cell Rep 2:185-97. 2012
    ..In addition, ORC1/CDC6 loss causes derepression of silent Variant Surface Glycoprotein genes, which are critical for host immune evasion...
  10. pmc Identification of ORC1/CDC6-interacting factors in Trypanosoma brucei reveals critical features of origin recognition complex architecture
    Calvin Tiengwe
    The Wellcome Trust Centre for Molecular Parasitology, Institute of Infection, Immunity and Inflammation, University of Glasgow, Glasgow, United Kingdom
    PLoS ONE 7:e32674. 2012
    ..brucei MCM subunits and show that this has the conventional eukaryotic heterohexameric structure, suggesting that divergence in the T. brucei replication machinery is limited to the earliest steps in origin licensing...
  11. ncbi request reprint Characterization of components of the mismatch repair machinery in Trypanosoma brucei
    Joanna S Bell
    The Wellcome Centre for Molecular Parasitology, University of Glasgow, Anderson College, 56 Dumbarton Road, Glasgow G11 6NU, UK
    Mol Microbiol 51:159-73. 2004
    ....
  12. doi request reprint Antigenic variation in the African trypanosome: molecular mechanisms and phenotypic complexity
    Liam J Morrison
    University of Glasgow, Wellcome Centre for Molecular Parasitology and Division of Infection and Immunity, Glasgow Biomedical Research Centre, 120 University Place, Glasgow, G12 8TA, UK
    Cell Microbiol 11:1724-34. 2009
    ....
  13. pmc Trypanosoma brucei homologous recombination is dependent on substrate length and homology, though displays a differential dependence on mismatch repair as substrate length decreases
    Rebecca L Barnes
    The Wellcome Centre for Molecular Parasitology, University of Glasgow, Glasgow Biomedical Research Centre, 120 University Place, Glasgow, G12 8TA, UK
    Nucleic Acids Res 35:3478-93. 2007
    ..Finally, we show that mismatches during T. brucei recombination may be repaired by short-patch mismatch repair...
  14. doi request reprint Nuclear DNA replication initiation in kinetoplastid parasites: new insights into an ancient process
    Calvin Tiengwe
    The University of Glasgow, Wellcome Trust Centre for Molecular Parasitology and Institute of Infection, Immunity and Inflammation, Sir Graeme Davis Building, 120 University Place, Glasgow, G12 8TA, UK The Johns Hopkins University School of Medicine, Department of Cell Biology, Baltimore, MD, USA
    Trends Parasitol 30:27-36. 2014
    ..In addition, we discuss how origin usage and replication dynamics relate to the highly unusual organisation of transcription in the genome of Trypanosoma brucei. ..
  15. ncbi request reprint Antigenic variation in African trypanosomes: monitoring progress
    Richard McCulloch
    Wellcome Centre for Molecular Parasitology, University of Glasgow, Anderson College, 56 Dumbarton Road, Glasgow, G11 6NU, UK
    Trends Parasitol 20:117-21. 2004
    ..Recent research progress in this field is highlighted here and some of the unresolved questions raised...
  16. doi request reprint Hydroxyurea-induced synchronisation of bloodstream stage Trypanosoma brucei
    Glynn R Forsythe
    Division of Infection and Immunity and Wellcome Centre for Molecular Parasitology, Glasgow Biomedical Research Centre, University of Glasgow, 120 University Place, Glasgow, Scotland, UK
    Mol Biochem Parasitol 164:131-6. 2009
    ..brucei...
  17. ncbi request reprint Two pathways of homologous recombination in Trypanosoma brucei
    Colin Conway
    The Wellcome Centre for Molecular Parasitology, The Anderson College, University of Glasgow, UK
    Mol Microbiol 45:1687-700. 2002
    ..We show that the RAD51-independent pathway is capable of recombining DNA substrates with very short lengths of sequence homology and in some cases aberrant recombination reactions can be detected using such microhomologies...