Harry P de Koning

Summary

Affiliation: University of Glasgow
Country: UK

Publications

  1. ncbi request reprint Drugs and drug resistance in African trypanosomiasis
    Vincent Delespaux
    Trypanosomosis Unit, Department of Animal Health, Institute of Tropical Medicine Antwerpen, Nationalestraat 155, B 2000 Antwerp, Belgium
    Drug Resist Updat 10:30-50. 2007
  2. ncbi request reprint Different substrate recognition motifs of human and trypanosome nucleobase transporters. Selective uptake of purine antimetabolites
    Lynsey J M Wallace
    Institute of Biomedical and Life Sciences, Division of Infection and Immunity, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, Scotland, United Kingdom
    J Biol Chem 277:26149-56. 2002
  3. ncbi request reprint Loss of the high-affinity pentamidine transporter is responsible for high levels of cross-resistance between arsenical and diamidine drugs in African trypanosomes
    Daniel J Bridges
    Institute of Biomedical and Life Sciences, Glasgow Biomedical Research Centre, University of Glasgow, Glasgow G12 8TA, United Kingdom
    Mol Pharmacol 71:1098-108. 2007
  4. ncbi request reprint Transporters in African trypanosomes: role in drug action and resistance
    H P De Koning
    Institute of Biomedical and Life Sciences, Division of Infection and Immunity, Joseph Black Building, University of Glasgow, G12 8QQ, Glasgow, UK
    Int J Parasitol 31:512-22. 2001
  5. ncbi request reprint Differential regulation of nucleoside and nucleobase transporters in Crithidia fasciculata and Trypanosoma brucei brucei
    H P De Koning
    Research School of Biosciences, University of Kent at Canterbury, UK
    Mol Biochem Parasitol 106:93-107. 2000
  6. ncbi request reprint Uptake of pentamidine in Trypanosoma brucei brucei is mediated by three distinct transporters: implications for cross-resistance with arsenicals
    H P De Koning
    Institute of Biomedical and Life Sciences, Division of Infection and Immunity, University of Glasgow, Glasgow, United Kingdom
    Mol Pharmacol 59:586-92. 2001
  7. ncbi request reprint Uptake of pentamidine in Trypanosoma brucei brucei is mediated by the P2 adenosine transporter and at least one novel, unrelated transporter
    H P De Koning
    Institute of Biomedical and Life Sciences, Division of Infection and Immunity, University of Glasgow, Glasgow G12 8QQ, UK
    Acta Trop 80:245-50. 2001
  8. doi request reprint Ever-increasing complexities of diamidine and arsenical crossresistance in African trypanosomes
    Harry P de Koning
    Institute of Biomedical and Life Sciences, Division of Infection and Immunity, Glasgow Biomedical Research Centre, University of Glasgow, Glasgow, UK
    Trends Parasitol 24:345-9. 2008
  9. ncbi request reprint Identification and characterisation of high affinity nucleoside and nucleobase transporters in Toxoplasma gondii
    Harry P de Koning
    Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, Joseph Black Building, Glasgow G12 8QQ, UK
    Int J Parasitol 33:821-31. 2003
  10. ncbi request reprint Purine and pyrimidine transport in pathogenic protozoa: from biology to therapy
    Harry P de Koning
    Institute of Biomedical and Life Sciences, Division of Infection and Immunity, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, UK
    FEMS Microbiol Rev 29:987-1020. 2005

Collaborators

Detail Information

Publications52

  1. ncbi request reprint Drugs and drug resistance in African trypanosomiasis
    Vincent Delespaux
    Trypanosomosis Unit, Department of Animal Health, Institute of Tropical Medicine Antwerpen, Nationalestraat 155, B 2000 Antwerp, Belgium
    Drug Resist Updat 10:30-50. 2007
    ....
  2. ncbi request reprint Different substrate recognition motifs of human and trypanosome nucleobase transporters. Selective uptake of purine antimetabolites
    Lynsey J M Wallace
    Institute of Biomedical and Life Sciences, Division of Infection and Immunity, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, Scotland, United Kingdom
    J Biol Chem 277:26149-56. 2002
    ..The potential for selective uptake of antimetabolites by the parasite transporter was demonstrated...
  3. ncbi request reprint Loss of the high-affinity pentamidine transporter is responsible for high levels of cross-resistance between arsenical and diamidine drugs in African trypanosomes
    Daniel J Bridges
    Institute of Biomedical and Life Sciences, Glasgow Biomedical Research Centre, University of Glasgow, Glasgow G12 8TA, United Kingdom
    Mol Pharmacol 71:1098-108. 2007
    ..It seems therefore that selection for resistance to either pentamidine or arsenical drugs can result in a similar phenotype of reduced drug accumulation, explaining the occurrence of cross-resistance...
  4. ncbi request reprint Transporters in African trypanosomes: role in drug action and resistance
    H P De Koning
    Institute of Biomedical and Life Sciences, Division of Infection and Immunity, Joseph Black Building, University of Glasgow, G12 8QQ, Glasgow, UK
    Int J Parasitol 31:512-22. 2001
    ..Lessons learned from studying the uptake of drugs currently in use may assist the design of a much needed new generation of trypanocides...
  5. ncbi request reprint Differential regulation of nucleoside and nucleobase transporters in Crithidia fasciculata and Trypanosoma brucei brucei
    H P De Koning
    Research School of Biosciences, University of Kent at Canterbury, UK
    Mol Biochem Parasitol 106:93-107. 2000
    ..These results show that regulation of the capacity of the purine transporters is common in different protozoa, and that, in T. b. brucei, various purine transporters are under differential control...
  6. ncbi request reprint Uptake of pentamidine in Trypanosoma brucei brucei is mediated by three distinct transporters: implications for cross-resistance with arsenicals
    H P De Koning
    Institute of Biomedical and Life Sciences, Division of Infection and Immunity, University of Glasgow, Glasgow, United Kingdom
    Mol Pharmacol 59:586-92. 2001
    ..Treatment with ionophores revealed that PPT1 may be a proton/pentamidine cotransporter...
  7. ncbi request reprint Uptake of pentamidine in Trypanosoma brucei brucei is mediated by the P2 adenosine transporter and at least one novel, unrelated transporter
    H P De Koning
    Institute of Biomedical and Life Sciences, Division of Infection and Immunity, University of Glasgow, Glasgow G12 8QQ, UK
    Acta Trop 80:245-50. 2001
    ..The identity of these transporters was investigated, and their significance for drug uptake and resistance in African trypanosomes is discussed...
  8. doi request reprint Ever-increasing complexities of diamidine and arsenical crossresistance in African trypanosomes
    Harry P de Koning
    Institute of Biomedical and Life Sciences, Division of Infection and Immunity, Glasgow Biomedical Research Centre, University of Glasgow, Glasgow, UK
    Trends Parasitol 24:345-9. 2008
    ....
  9. ncbi request reprint Identification and characterisation of high affinity nucleoside and nucleobase transporters in Toxoplasma gondii
    Harry P de Koning
    Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, Joseph Black Building, Glasgow G12 8QQ, UK
    Int J Parasitol 33:821-31. 2003
    ..gondii...
  10. ncbi request reprint Purine and pyrimidine transport in pathogenic protozoa: from biology to therapy
    Harry P de Koning
    Institute of Biomedical and Life Sciences, Division of Infection and Immunity, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, UK
    FEMS Microbiol Rev 29:987-1020. 2005
    ..Recent studies are increasingly addressing the structure and substrate recognition mechanisms of these vital transport proteins...
  11. ncbi request reprint Cloning, heterologous expression, and in situ characterization of the first high affinity nucleobase transporter from a protozoan
    Richard J S Burchmore
    Institute of Biomedical and Life Sciences, Division of Infection and Immunity, University of Glasgow, Glasgow G12 8QQ, United Kingdom
    J Biol Chem 278:23502-7. 2003
    ..Expression of TbNBT1 in Xenopus oocytes further confirmed that this gene encodes the first high affinity nucleobase transporter from protozoa or animals to be identified at the molecular level...
  12. pmc Purine nucleobase transport in amastigotes of Leishmania mexicana: involvement in allopurinol uptake
    Mohammed I Al-Salabi
    Institute of Biomedical and Life Sciences, Division of Infection and Immunity, Joseph Black Building, University of Glasgow, Glasgow G12 8QQ, United Kingdom
    Antimicrob Agents Chemother 49:3682-9. 2005
    ..The binding interactions were compared with those of the LmajNBT1 transporter in Leishmania major promastigotes and found to be very similar...
  13. ncbi request reprint Molecular interactions underlying the unusually high adenosine affinity of a novel Trypanosoma brucei nucleoside transporter
    Mohammed I Al-Salabi
    Institute of Biomedical and Life Sciences, Glasgow Biomedical Research Centre, University of Glasgow, Glasgow G12 8TA, United Kingdom
    Mol Pharmacol 71:921-9. 2007
    ..We show that both TbNT9/AT-D and NT10/AT-B transport a range of potentially therapeutic nucleoside analogs...
  14. doi request reprint Validation of novel fluorescence assays for the routine screening of drug susceptibilities of Trichomonas vaginalis
    Manal J Natto
    Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G12 8TA, UK
    J Antimicrob Chemother 67:933-43. 2012
    ..To evaluate existing protocols, based on Alamar blue (resazurin), for the routine determination of drug susceptibility in trichomonads, develop new ones and validate these by screening small antiprotozoal libraries...
  15. ncbi request reprint Trypanosoma brucei: expression of multiple purine transporters prevents the development of allopurinol resistance
    Manal J Natto
    Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, Joseph Black Building, Glasgow G12 8QQ, UK
    Exp Parasitol 109:80-6. 2005
    ..This study shows that transport-related resistance to purine antimetabolites is not easily induced in Trypanosoma spp. as long as uptake is mediated by multiple transporters...
  16. pmc The trypanocide diminazene aceturate is accumulated predominantly through the TbAT1 purine transporter: additional insights on diamidine resistance in african trypanosomes
    Harry P de Koning
    Institute of Biomedical and Life Sciences, Division of Infection and Immunity, University of Glasgow, Glasgow G12 8QQ, United Kingdom
    Antimicrob Agents Chemother 48:1515-9. 2004
    ..We conclude that TbAT1 mediates [(3)H]diminazene transport almost exclusively and that this explains the observed diminazene resistance phenotypes of TbAT1-null mutants and field isolates...
  17. pmc Multiple genetic mechanisms lead to loss of functional TbAT1 expression in drug-resistant trypanosomes
    Mhairi L Stewart
    Division of Infection and Immunity, Faculty of Biomedical and Life Sciences, Glasgow Biomedical Research Centre, University of Glasgow, Glasgow G12 8TA, United Kingdom
    Eukaryot Cell 9:336-43. 2010
    ..b. brucei mutant, although at a greatly reduced capacity compared to that of the wild type, indicating that an additional adenine-inhibitable adenosine permease, distinct from P2, is present in these cells...
  18. ncbi request reprint A Leishmania major nucleobase transporter responsible for allopurinol uptake is a functional homolog of the Trypanosoma brucei H2 transporter
    Mohammed I Al-Salabi
    Institute of Biomedical and Life Sciences, Division of Infection and Immunity, Joseph Black Building, University of Glasgow, Glasgow, United Kingdom
    Mol Pharmacol 63:814-20. 2003
    ..It is proposed that the architecture of their respective binding sites is very similar and that LmNBT1 can be named a functional homolog of TbH2...
  19. pmc Cyclic AMP effectors in African trypanosomes revealed by genome-scale RNA interference library screening for resistance to the phosphodiesterase inhibitor CpdA
    Matthew K Gould
    Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom
    Antimicrob Agents Chemother 57:4882-93. 2013
    ..As cAMP metabolism is validated as a drug target in Trypanosoma brucei, cAMP effectors highly divergent from the mammalian host, such as CARP1, lend themselves to further pharmacological development. ..
  20. pmc Pyrimidine salvage in Trypanosoma brucei bloodstream forms and the trypanocidal action of halogenated pyrimidines
    Juma A M Ali
    Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G12 8TA, United Kingdom
    Mol Pharmacol 83:439-53. 2013
    ..We present the most complete model of pyrimidine salvage in T. brucei to date, supported by genome-wide profiling of the predicted pyrimidine biosynthesis and conversion enzymes...
  21. pmc Uptake of purines in Plasmodium falciparum-infected human erythrocytes is mostly mediated by the human equilibrative nucleoside transporter and the human facilitative nucleobase transporter
    Neils B Quashie
    Institute of Biomedical and Life Sciences, Division of Infection and Immunity, Glasgow Biomedical Research Centre, University of Glasgow, Glasgow G128TA, UK
    Malar J 9:36. 2010
    ..Using the appropriate transporter inhibitors, the role of each of these salvage pathways to the overall purine transport in intraerythrocytic Plasmodium falciparum was systematically investigated...
  22. ncbi request reprint Trypanosoma brucei: a survey of pyrimidine transport activities
    Simon Gudin
    Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, Biomedical Research Center, Glasgow G12 8TA, UK
    Exp Parasitol 114:118-25. 2006
    ..Pyrimidine antimetabolites were tested as potential trypanocidal agents and only 5-fluorouracil was found to be effective. This drug was efficiently taken up by bloodstream forms of T. b. brucei...
  23. pmc The diamidine diminazene aceturate is a substrate for the high-affinity pentamidine transporter: implications for the development of high resistance levels in trypanosomes
    Ibrahim A Teka
    Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom
    Mol Pharmacol 80:110-6. 2011
    ....
  24. doi request reprint A comprehensive model of purine uptake by the malaria parasite Plasmodium falciparum: identification of four purine transport activities in intraerythrocytic parasites
    Neils B Quashie
    Institute of Biomedical and Life Sciences, Division of Infection and Immunity, Glasgow G12 8TA, Scotland, UK
    Biochem J 411:287-95. 2008
    ..We thus propose a model for purine salvage in P. falciparum, based on the highly efficient uptake of hypoxanthine by PfNT1 and a high capacity for purine nucleoside uptake by a lower affinity carrier...
  25. ncbi request reprint Detection of arsenical drug resistance in Trypanosoma brucei with a simple fluorescence test
    Mhairi L Stewart
    University of Glasgow, Division of Infection and Immunity, Institute of Biomedical and Life Sciences, The Joseph Black Building, Glasgow G12 8QQ, UK
    Lancet 366:486-7. 2005
    ..The two DNA-containing structures in the trypanosome--the nucleus and the kinetoplast--begin to fluoresce within 1 min of introduction of DB99, unless drug resistant...
  26. pmc Trypanocidal action of bisphosphonium salts through a mitochondrial target in bloodstream form Trypanosoma brucei
    Abdulsalam A M Alkhaldi
    Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom
    Int J Parasitol Drugs Drug Resist 6:23-34. 2016
    ..The results show that, in T. brucei, bisphosphonium compounds do not principally act on succinate dehydrogenase but on the mitochondrial FoF1 ATPase...
  27. pmc Pyrimidine biosynthesis is not an essential function for Trypanosoma brucei bloodstream forms
    Juma A M Ali
    Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom
    PLoS ONE 8:e58034. 2013
    ..African trypanosomes are capable of both pyrimidine biosynthesis and salvage of preformed pyrimidines from the host, but it is unknown whether either process is essential to the parasite...
  28. ncbi request reprint Characterisation of the plasma membrane subproteome of bloodstream form Trypanosoma brucei
    Daniel J Bridges
    Division of Infection and Immunity, Institute of Biomedical and Life Sciences, University of Glasgow, Glasgow, UK
    Proteomics 8:83-99. 2008
    ....
  29. pmc Pharmacological validation of Trypanosoma brucei phosphodiesterases as novel drug targets
    Harry P de Koning
    Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, United Kingdom
    J Infect Dis 206:229-37. 2012
    ..Cpd A prevents cytokinesis, resulting in multinucleated, multiflagellated cells that eventually lyse. These observations pharmacologically validate the highly conserved trypanosomal PDEs as potential drug targets...
  30. doi request reprint A fluorescence-based assay for the uptake of CPD0801 (DB829) by African trypanosomes
    Christopher P Ward
    Division of Infection and Immunity, Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow, UK
    Mol Biochem Parasitol 174:145-9. 2010
    ..Our method is directly applicable to the study of other diamidines with similar fluorescent properties and readily adapted for use with other cell types or fluorescent molecules as we demonstrate for the veterinary trypanocide ethidium...
  31. pmc Symmetrical choline-derived dications display strong anti-kinetoplastid activity
    Hasan M S Ibrahim
    Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G12 8TA, UK
    J Antimicrob Chemother 66:111-25. 2011
    ..to investigate the anti-kinetoplastid activity of choline-derived analogues with previously reported antimalarial efficacy...
  32. ncbi request reprint Adenosine transporters in bloodstream forms of Trypanosoma brucei brucei: substrate recognition motifs and affinity for trypanocidal drugs
    H P De Koning
    Research School of Biosciences, University of Kent at Canterbury, Canterbury, Kent, UK
    Mol Pharmacol 56:1162-70. 1999
    ..4-2.4 microM)...
  33. pmc Reduced Mitochondrial Membrane Potential Is a Late Adaptation of Trypanosoma brucei brucei to Isometamidium Preceded by Mutations in the γ Subunit of the F1Fo-ATPase
    Anthonius A Eze
    Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom
    PLoS Negl Trop Dis 10:e0004791. 2016
    ..b. gambiense and T. b. rhodesiense. Resistance to isometamidium is a growing concern, as is cross-resistance to the diamidine drugs diminazene and pentamidine...
  34. doi request reprint Potent trypanocidal curcumin analogs bearing a monoenone linker motif act on trypanosoma brucei by forming an adduct with trypanothione
    Abdulsalam A M Alkhaldi
    Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, United Kingdom A A M A, D J C, H I, D H K, N B Q, K E B, M P B, H P K Department of Biology, College of Science, Aljouf University, Skaka, Kingdom of Saudi Arabia A A M A Department of Biochemistry and Molecular Biology, Bio21 Institute, University of Melbourne, Australia D J C Faculty of Science, Department of Zoology, Sebha University, Libya H I Centre for Analytical Bioscience, School of Pharmacy, University of Nottingham, Nottingham, United Kingdom D H K Centre for Tropical Clinical Pharmacology and Therapeutics, University of Ghana Medical School, Accra, Ghana N B Q Division of Physical Science, Faculty of Science and Technology, Huachiew Chalermprakiet University, Samutprakarn, Thailand C C Wellcome Trust Centre for Molecular Parasitology, University of Glasgow, Glasgow, United Kingdom M P B and Department of Chemistry and Center of Excellence for Innovation in Chemistry, USA
    Mol Pharmacol 87:451-64. 2015
    ..This adduct may ultimately be responsible for the highly potent trypanocidal and antiparasitic activity of the monoenone curcuminoids...
  35. pmc Trypanosoma brucei aquaglyceroporin 2 is a high-affinity transporter for pentamidine and melaminophenyl arsenic drugs and the main genetic determinant of resistance to these drugs
    Jane C Munday
    Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK
    J Antimicrob Chemother 69:651-63. 2014
    ..We recently reported that loss of T. brucei aquaglyceroporin 2 (TbAQP2) caused melarsoprol/pentamidine cross-resistance (MPXR) in these parasites and the current study aims to delineate the mechanism by which this occurs...
  36. pmc An improved and highly sensitive microfluorimetric method for assessing susceptibility of Plasmodium falciparum to antimalarial drugs in vitro
    Neils B Quashie
    Institute of Biomedical and Life Sciences, Division of Infection and Immunity, Glasgow Biomedical Research Centre, University of Glasgow, 120 University Place, Glasgow G12 8TA, Scotland, UK
    Malar J 5:95. 2006
    ....
  37. doi request reprint Propidium iodide-based methods for monitoring drug action in the kinetoplastidae: comparison with the Alamar Blue assay
    Matthew K Gould
    Institute of Biomedical and Life Sciences, Division of Infection and Immunity, University of Glasgow, 120 University Place, Glasgow G128TA, UK
    Anal Biochem 382:87-93. 2008
    ..The EC(50) values were highly similar to those obtained with the standard Alamar Blue assay. The procedure lends itself readily to applications in drug development or resistance monitoring...
  38. pmc Trypanocidal furamidine analogues: influence of pyridine nitrogens on trypanocidal activity, transport kinetics, and resistance patterns
    Christopher P Ward
    Wellcome Trust Centre for Molecular Parasitology, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, 120 University Place, Glasgow G12 8TA, United Kingdom
    Antimicrob Agents Chemother 55:2352-61. 2011
    ..The aza analogues of DB75 accumulate more slowly than furamidine itself and reveal less trypanocidal activity in standard in vitro drug sensitivity assays...
  39. doi request reprint Chemical characterisation of Nigerian red propolis and its biological activity against Trypanosoma Brucei
    Ruwida M K Omar
    Strathclyde Institute of Pharmacy and Biomedical Sciences, University of Strathclyde, 27 Taylor Street, Glasgow, G4 0NR, UK
    Phytochem Anal 27:107-15. 2016
    ..A previous study showed the unique character of Nigerian red propolis from Rivers State, Nigeria (RSN), with regards to chemical composition and activity against Trypanosoma brucei in comparison with other African propolis...
  40. pmc Functional analysis of drug resistance-associated mutations in the Trypanosoma brucei adenosine transporter 1 (TbAT1) and the proposal of a structural model for the protein
    Jane C Munday
    Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, G12 8TA, UK
    Mol Microbiol 96:887-900. 2015
    ..The results presented here validate earlier hypotheses of drug binding motifs for TbAT1...
  41. pmc Targeting the parasite's DNA with methyltriazenyl purine analogs is a safe, selective, and efficacious antitrypanosomal strategy
    Boris Rodenko
    College of Medical, Veterinary and Life Sciences, Institute of Infection, Immunity and Inflammation, University of Glasgow, Glasgow, United Kingdom Wellcome Trust Centre for Molecular Parasitology, University of Glasgow, Glasgow, United Kingdom
    Antimicrob Agents Chemother 59:6708-16. 2015
    ....
  42. pmc Chemical and Antimicrobial Profiling of Propolis from Different Regions within Libya
    Weam Siheri
    University of Strathclyde, Strathclyde Institute of Pharmacy and Biomedical Science, 161 Cathedral Street, Glasgow, G4 0RE, United Kingdom
    PLoS ONE 11:e0155355. 2016
    ..Orthogonal partial least squares (OPLS) analysis was used to link the activity of each extract against the different micro-organisms to particular components in the extracts. ..
  43. pmc The animal trypanosomiases and their chemotherapy: a review
    Federica Giordani
    Wellcome Trust Centre for Molecular Parasitology, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow G12 8TA, UK
    Parasitology 143:1862-1889. 2016
    ....
  44. doi request reprint Protocols for the routine screening of drug sensitivity in the human parasite Trichomonas vaginalis
    Manal J Natto
    Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, 120 University Place, Glasgow, G12 8TA, UK
    Methods Mol Biol 1263:103-10. 2015
    ..Here we report on two separate in vitro protocols that use fluorescent dyes and allow for standardized drug sensitivity testing on the required scale. ..
  45. doi request reprint Cyclic-nucleotide signalling in protozoa
    Matthew K Gould
    Biomedical Research Centre, Institute of Infection, Immunity and Inflammation, College of Medical, Veterinary and Life Sciences, University of Glasgow, Glasgow, UK
    FEMS Microbiol Rev 35:515-41. 2011
    ..Some of the PDEs have now been validated as promising drug targets. In the following manuscript, we will summarize the existing literature on cAMP and cGMP in protozoa: cyclases, PDEs and cyclic-nucleotide-dependent kinases...
  46. ncbi request reprint Selective transport of a new class of purine antimetabolites by the protozoan parasite Trypanosoma brucei
    L J M Wallace
    Institute of Biomedical and Life Sciences, Division of Infection and Immunity, University of Glasgow, Glasgow, UK
    Nucleosides Nucleotides Nucleic Acids 23:1441-4. 2004
    ..We show that these compounds display a remarkable selectivity for the parasite's transporters. The adenine analogue showed greater trypanocidal activity than the hypoxanthine or guanine analogues in vitro...
  47. ncbi request reprint Characterization of the choline carrier of Plasmodium falciparum: a route for the selective delivery of novel antimalarial drugs
    Giancarlo A Biagini
    Molecular and Biochemical Parasitology Group, Liverpool School of Tropical Medicine, England
    Blood 104:3372-7. 2004
    ....
  48. ncbi request reprint Pentamidine uptake and resistance in pathogenic protozoa: past, present and future
    Patrick G Bray
    Molecular and Biochemical Parasitology Group, Liverpool School of Tropical Medicine, Liverpool, L3 5QS, UK
    Trends Parasitol 19:232-9. 2003
    ....
  49. pmc Mechanisms of arsenical and diamidine uptake and resistance in Trypanosoma brucei
    Enock Matovu
    Institute of Cell Biology, CH 3012 Bern, Switzerland
    Eukaryot Cell 2:1003-8. 2003
    ..High-level arsenical resistance therefore appears to involve the loss of more than one transporter...
  50. ncbi request reprint Molecular pharmacology of adenosine transport in Trypanosoma brucei: P1/P2 revisited
    Federico Geiser
    Institute of Cell Biology, Baltzerstrasse 4, CH 3012 Bern, Switzerland
    Mol Pharmacol 68:589-95. 2005
    ..Overexpression of P1 by trypanosomes after loss of P2 indicates that combinatorial chemotherapy with trypanocidal P1 and P2 substrates may be a promising strategy to prevent drug resistance in sleeping sickness...
  51. ncbi request reprint Biosynthesis and uptake of thiamine (vitamin B1) in bloodstream form Trypanosoma brucei brucei and interference of the vitamin with melarsen oxide activity
    Sabine A Stoffel
    Institute of Cell Biology, University of Bern, Baltzerstrasse 4, 3012 Bern, Switzerland
    Int J Parasitol 36:229-36. 2006
    ..brucei. These effects were consistent with a low affinity of thiamine for the P2 adenosine transporter that is responsible for uptake of melaminophenyl arsenicals in African trypanosomes...
  52. pmc 2,N6-disubstituted adenosine analogs with antitrypanosomal and antimalarial activities
    Boris Rodenko
    van t Hoff Institute for Molecular Sciences, Universiteit van Amsterdam, The Netherlands
    Antimicrob Agents Chemother 51:3796-802. 2007
    ..b. brucei strain lacking the P2 nucleoside transporter as they were against the parental strain. As the analogs were not toxic to human cell lines, the purine analogs are likely to act on a trypanosome-specific target...