Richard McCulloch

Summary

Affiliation: University of Glasgow
Country: UK

Publications

  1. pmc Trypanosoma brucei homologous recombination is dependent on substrate length and homology, though displays a differential dependence on mismatch repair as substrate length decreases
    Rebecca L Barnes
    The Wellcome Centre for Molecular Parasitology, University of Glasgow, Glasgow Biomedical Research Centre, 120 University Place, Glasgow, G12 8TA, UK
    Nucleic Acids Res 35:3478-93. 2007
  2. pmc Distinct roles for two RAD51-related genes in Trypanosoma brucei antigenic variation
    Chris Proudfoot
    The Wellcome Centre for Molecular Parasitology, University of Glasgow, Anderson College, 56 Dumbarton Road, Glasgow, G11 6NU, UK
    Nucleic Acids Res 33:6906-19. 2005
  3. doi request reprint What has DNA sequencing revealed about the VSG expression sites of African trypanosomes?
    Richard McCulloch
    University of Glasgow, Glasgow Biomedical Research Centre, UK
    Trends Parasitol 25:359-63. 2009
  4. pmc A role for RAD51 and homologous recombination in Trypanosoma brucei antigenic variation
    R McCulloch
    The Wellcome Centre for Molecular Parasitology, The Anderson College, University of Glasgow, Glasgow G11 6NU, U K
    Genes Dev 13:2875-88. 1999
  5. ncbi request reprint Antigenic variation in African trypanosomes: monitoring progress
    Richard McCulloch
    Wellcome Centre for Molecular Parasitology, University of Glasgow, Anderson College, 56 Dumbarton Road, Glasgow, G11 6NU, UK
    Trends Parasitol 20:117-21. 2004
  6. ncbi request reprint Inactivation of Mre11 does not affect VSG gene duplication mediated by homologous recombination in Trypanosoma brucei
    Nicholas P Robinson
    Wellcome Centre for Molecular Parasitology, University of Glasgow, Anderson College, 56 Dumbarton Road, Glasgow G11 6NU, Scotland, United Kingdom
    J Biol Chem 277:26185-93. 2002
  7. pmc Ku heterodimer-independent end joining in Trypanosoma brucei cell extracts relies upon sequence microhomology
    Peter Burton
    The Wellcome Centre for Molecular Parasitology, University of Glasgow, Glasgow Biomedical Research Centre, Glasgow, Scotland, UK
    Eukaryot Cell 6:1773-81. 2007
  8. ncbi request reprint Two pathways of homologous recombination in Trypanosoma brucei
    Colin Conway
    The Wellcome Centre for Molecular Parasitology, The Anderson College, University of Glasgow, UK
    Mol Microbiol 45:1687-700. 2002
  9. ncbi request reprint Mismatch repair regulates homologous recombination, but has little influence on antigenic variation, in Trypanosoma brucei
    Joanna S Bell
    Wellcome Centre for Molecular Parasitology, University of Glasgow, Anderson College, 56 Dumbarton Road, Glasgow G11 6NU, Scotland, United Kingdom
    J Biol Chem 278:45182-8. 2003
  10. ncbi request reprint Trypanosoma brucei DMC1 does not act in DNA recombination, repair or antigenic variation in bloodstream stage cells
    Chris Proudfoot
    The Wellcome Centre for Molecular Parasitology, University of Glasgow, Anderson College, 56 Dumbarton Road, Glasgow G11 6NU, UK
    Mol Biochem Parasitol 145:245-53. 2006

Collaborators

Detail Information

Publications35

  1. pmc Trypanosoma brucei homologous recombination is dependent on substrate length and homology, though displays a differential dependence on mismatch repair as substrate length decreases
    Rebecca L Barnes
    The Wellcome Centre for Molecular Parasitology, University of Glasgow, Glasgow Biomedical Research Centre, 120 University Place, Glasgow, G12 8TA, UK
    Nucleic Acids Res 35:3478-93. 2007
    ..Finally, we show that mismatches during T. brucei recombination may be repaired by short-patch mismatch repair...
  2. pmc Distinct roles for two RAD51-related genes in Trypanosoma brucei antigenic variation
    Chris Proudfoot
    The Wellcome Centre for Molecular Parasitology, University of Glasgow, Anderson College, 56 Dumbarton Road, Glasgow, G11 6NU, UK
    Nucleic Acids Res 33:6906-19. 2005
    ..brucei have assumed specialized functions in homologous recombination, analogous to related proteins in metazoan eukaryotes...
  3. doi request reprint What has DNA sequencing revealed about the VSG expression sites of African trypanosomes?
    Richard McCulloch
    University of Glasgow, Glasgow Biomedical Research Centre, UK
    Trends Parasitol 25:359-63. 2009
    ..This gap in our knowledge has now been bridged by two new studies, which we discuss here, asking what has been revealed about the biological significance of BES multiplicity and ESAG function and evolution...
  4. pmc A role for RAD51 and homologous recombination in Trypanosoma brucei antigenic variation
    R McCulloch
    The Wellcome Centre for Molecular Parasitology, The Anderson College, University of Glasgow, Glasgow G11 6NU, U K
    Genes Dev 13:2875-88. 1999
    ..Switching events were still detectable, however, so it appears that trypanosome factors other than RAD51 can also provide for antigenic variation...
  5. ncbi request reprint Antigenic variation in African trypanosomes: monitoring progress
    Richard McCulloch
    Wellcome Centre for Molecular Parasitology, University of Glasgow, Anderson College, 56 Dumbarton Road, Glasgow, G11 6NU, UK
    Trends Parasitol 20:117-21. 2004
    ..Recent research progress in this field is highlighted here and some of the unresolved questions raised...
  6. ncbi request reprint Inactivation of Mre11 does not affect VSG gene duplication mediated by homologous recombination in Trypanosoma brucei
    Nicholas P Robinson
    Wellcome Centre for Molecular Parasitology, University of Glasgow, Anderson College, 56 Dumbarton Road, Glasgow G11 6NU, Scotland, United Kingdom
    J Biol Chem 277:26185-93. 2002
    ..Mre11 inactivation appears not to affect VSG gene switching during antigenic variation of a laboratory strain, which is perhaps surprising given the importance of homologous recombination during this process...
  7. pmc Ku heterodimer-independent end joining in Trypanosoma brucei cell extracts relies upon sequence microhomology
    Peter Burton
    The Wellcome Centre for Molecular Parasitology, University of Glasgow, Glasgow Biomedical Research Centre, Glasgow, Scotland, UK
    Eukaryot Cell 6:1773-81. 2007
    ..brucei, in common with other kinetoplastids, does not encode recognizable homologues of DNA ligase IV or XRCC4, suggesting that NHEJ is either absent or mechanistically diverged in these pathogens...
  8. ncbi request reprint Two pathways of homologous recombination in Trypanosoma brucei
    Colin Conway
    The Wellcome Centre for Molecular Parasitology, The Anderson College, University of Glasgow, UK
    Mol Microbiol 45:1687-700. 2002
    ..We show that the RAD51-independent pathway is capable of recombining DNA substrates with very short lengths of sequence homology and in some cases aberrant recombination reactions can be detected using such microhomologies...
  9. ncbi request reprint Mismatch repair regulates homologous recombination, but has little influence on antigenic variation, in Trypanosoma brucei
    Joanna S Bell
    Wellcome Centre for Molecular Parasitology, University of Glasgow, Anderson College, 56 Dumbarton Road, Glasgow G11 6NU, Scotland, United Kingdom
    J Biol Chem 278:45182-8. 2003
    ....
  10. ncbi request reprint Trypanosoma brucei DMC1 does not act in DNA recombination, repair or antigenic variation in bloodstream stage cells
    Chris Proudfoot
    The Wellcome Centre for Molecular Parasitology, University of Glasgow, Anderson College, 56 Dumbarton Road, Glasgow G11 6NU, UK
    Mol Biochem Parasitol 145:245-53. 2006
    ..DMC1 mutation does not, however, result in detectable alterations in DNA repair, recombination or antigenic variation efficiency in this life cycle stage...
  11. pmc Mapping replication dynamics in Trypanosoma brucei reveals a link with telomere transcription and antigenic variation
    Rebecca Devlin
    The Wellcome Trust Centre for Molecular Parasitology, Institute of Infection, Immunity and Inflammation, University of Glasgow, Glasgow, United Kingdom
    elife 5:. 2016
    ..Specific association between VSG transcription and replication timing reveals a model for antigenic variation based on replication-derived DNA fragility. ..
  12. ncbi request reprint Ku is important for telomere maintenance, but not for differential expression of telomeric VSG genes, in African trypanosomes
    Colin Conway
    Wellcome Centre for Molecular Parasitology, University of Glasgow, Anderson College, 56 Dumbarton Road, Glasgow, G11 6NU, Scotland, United Kingdom
    J Biol Chem 277:21269-77. 2002
    ..The absence of Ku and the consequent great shortening of telomeres had no detectable influence either on the rate of VSG switching or on the silencing of the telomeric promoters of the VSG subset that is expressed in the tsetse fly...
  13. pmc Trypanosoma brucei BRCA2 acts in antigenic variation and has undergone a recent expansion in BRC repeat number that is important during homologous recombination
    Claire L Hartley
    The Wellcome Centre for Molecular Parasitology and Faculty of Biomedical and Life Sciences, University of Glasgow, Glasgow Biomedical Research Centre, 120 University Place, Glasgow G12 8TA, UK
    Mol Microbiol 68:1237-51. 2008
    ..brucei homologous recombination and RAD51 localization. Remarkably, however, this appears not to be a major determinant of the activation of at least some VSG genes...
  14. pmc Genome-wide analysis reveals extensive functional interaction between DNA replication initiation and transcription in the genome of Trypanosoma brucei
    Calvin Tiengwe
    The Wellcome Trust Centre for Molecular Parasitology, College of Medical, Veterinary and Life Sciences, Institute of Infection, Immunity and Inflammation, University of Glasgow, Sir Graeme Davies Building, 120 University Place, Glasgow G12 8TA, UK
    Cell Rep 2:185-97. 2012
    ..In addition, ORC1/CDC6 loss causes derepression of silent Variant Surface Glycoprotein genes, which are critical for host immune evasion...
  15. pmc Trypanosoma brucei BRCA2 acts in a life cycle-specific genome stability process and dictates BRC repeat number-dependent RAD51 subnuclear dynamics
    Anna Trenaman
    The Wellcome Trust Centre for Molecular Parasitology, College of Medical Veterinary and Life Sciences, Institute of Infection, Immunity and Inflammation, University of Glasgow, Sir Graeme Davies Building, 120 University Place, Glasgow G12 8TA, UK
    Nucleic Acids Res 41:943-60. 2013
    ..brucei, and this function has necessitated the evolution of extensive RAD51 interaction via the BRC repeats, allowing re-localization of the recombinase to general genome damage when needed...
  16. pmc Identification of ORC1/CDC6-interacting factors in Trypanosoma brucei reveals critical features of origin recognition complex architecture
    Calvin Tiengwe
    The Wellcome Trust Centre for Molecular Parasitology, Institute of Infection, Immunity and Inflammation, University of Glasgow, Glasgow, United Kingdom
    PLoS ONE 7:e32674. 2012
    ..brucei MCM subunits and show that this has the conventional eukaryotic heterohexameric structure, suggesting that divergence in the T. brucei replication machinery is limited to the earliest steps in origin licensing...
  17. pmc Interactions among Trypanosoma brucei RAD51 paralogues in DNA repair and antigenic variation
    Rachel Dobson
    College of Medical Veterinary and Life Sciences, University of Glasgow, Institute of Infection, Immunity and Inflammation, The Wellcome Trust Centre for Molecular Parasitology, Sir Graeme Davis Building, 120 University Place, Glasgow G128TA, UK
    Mol Microbiol 81:434-56. 2011
    ..brucei is unusually large among microbial eukaryotes and that one member of the protein family corresponds with a key, conserved eukaryotic Rad51 paralogue...
  18. ncbi request reprint Characterization of components of the mismatch repair machinery in Trypanosoma brucei
    Joanna S Bell
    The Wellcome Centre for Molecular Parasitology, University of Glasgow, Anderson College, 56 Dumbarton Road, Glasgow G11 6NU, UK
    Mol Microbiol 51:159-73. 2004
    ....
  19. doi request reprint Antigenic variation in the African trypanosome: molecular mechanisms and phenotypic complexity
    Liam J Morrison
    University of Glasgow, Wellcome Centre for Molecular Parasitology and Division of Infection and Immunity, Glasgow Biomedical Research Centre, 120 University Place, Glasgow, G12 8TA, UK
    Cell Microbiol 11:1724-34. 2009
    ....
  20. pmc Diverged composition and regulation of the Trypanosoma brucei origin recognition complex that mediates DNA replication initiation
    Catarina A Marques
    The Wellcome Trust Centre for Molecular Parasitology, Institute of Infection, Immunity and Inflammation, University of Glasgow, Sir Graeme Davis Building, 120 University Place, Glasgow, G12 8TA, UK
    Nucleic Acids Res 44:4763-84. 2016
    ..This work shows that ORC architecture and regulation are diverged features of DNA replication initiation in T. brucei, providing new insight into this key stage of eukaryotic genome copying. ..
  21. doi request reprint Nuclear DNA replication initiation in kinetoplastid parasites: new insights into an ancient process
    Calvin Tiengwe
    The University of Glasgow, Wellcome Trust Centre for Molecular Parasitology and Institute of Infection, Immunity and Inflammation, Sir Graeme Davis Building, 120 University Place, Glasgow, G12 8TA, UK The Johns Hopkins University School of Medicine, Department of Cell Biology, Baltimore, MD, USA
    Trends Parasitol 30:27-36. 2014
    ..In addition, we discuss how origin usage and replication dynamics relate to the highly unusual organisation of transcription in the genome of Trypanosoma brucei. ..
  22. ncbi request reprint Why are parasite contingency genes often associated with telomeres?
    J D Barry
    Wellcome Centre for Molecular Parasitology, University of Glasgow, Anderson College, 56 Dumbarton Road, UK
    Int J Parasitol 33:29-45. 2003
    ..We examine, in several antigenic variation systems, what possible benefits apply...
  23. ncbi request reprint Stable transformation of trypanosomatids through targeted chromosomal integration of the selectable marker gene encoding blasticidin S deaminase
    D R Brooks
    Wellcome Centre for Molecular Parasitology, University of Glasgow, The Anderson College, Glasgow, UK
    FEMS Microbiol Lett 186:287-91. 2000
    ..The results confirm that BSD can be used as a selectable marker gene for targeted chromosomal integration during genetic manipulations of trypanosomatids...
  24. ncbi request reprint Does DNA replication direct locus-specific recombination during host immune evasion by antigenic variation in the African trypanosome?
    Rebecca Devlin
    The Wellcome Trust Centre for Molecular Parasitology, Institute of Infection, Immunity and Inflammation, University of Glasgow, Sir Graeme Davis Building, 120 University Place, Glasgow, G12 8TA, UK
    Curr Genet . 2016
    ..Here, we discuss three emerging models for VSG switching initiation and ask how these compare with processes for adaptive genome change found in other organisms...
  25. pmc Distinct Phenotypes Caused by Mutation of MSH2 in Trypanosome Insect and Mammalian Life Cycle Forms Are Associated with Parasite Adaptation to Oxidative Stress
    Viviane Grazielle-Silva
    Departamento de Bioquimica e Imunologia, Universidade Federal de Minas Gerais, Belo Horizonte, Minas Gerais, Brazil The Wellcome Trust Center for Molecular Parasitology, Institute of Infection, Immunity and Inflammation, University of Glasgow, Glasgow, Scotland, United Kingdom
    PLoS Negl Trop Dis 9:e0003870. 2015
    ..In addition, recent evidence suggests that MSH2 might also play an important, but poorly understood, role in responding to oxidative damage in both African and American trypanosomes...
  26. pmc Genome-wide mapping reveals single-origin chromosome replication in Leishmania, a eukaryotic microbe
    Catarina A Marques
    The Wellcome Trust Centre for Molecular Parasitology, Institute of Infection, Immunity and Inflammation, University of Glasgow, Sir Graeme Davis Building, 120 University Place, Glasgow, G12 8TA, UK
    Genome Biol 16:230. 2015
    ..To ask if these features of DNA replication are true of all eukaryotes, we describe genome-wide origin mapping in the parasite Leishmania...
  27. pmc Targeting the parasite's DNA with methyltriazenyl purine analogs is a safe, selective, and efficacious antitrypanosomal strategy
    Boris Rodenko
    College of Medical, Veterinary and Life Sciences, Institute of Infection, Immunity and Inflammation, University of Glasgow, Glasgow, United Kingdom Wellcome Trust Centre for Molecular Parasitology, University of Glasgow, Glasgow, United Kingdom
    Antimicrob Agents Chemother 59:6708-16. 2015
    ....
  28. doi request reprint Hydroxyurea-induced synchronisation of bloodstream stage Trypanosoma brucei
    Glynn R Forsythe
    Division of Infection and Immunity and Wellcome Centre for Molecular Parasitology, Glasgow Biomedical Research Centre, University of Glasgow, 120 University Place, Glasgow, Scotland, UK
    Mol Biochem Parasitol 164:131-6. 2009
    ..brucei...
  29. ncbi request reprint Antigenic variation in trypanosomes: enhanced phenotypic variation in a eukaryotic parasite
    J D Barry
    Wellcome Centre for Molecular Parasitology, University of Glasgow, Scotland, UK
    Adv Parasitol 49:1-70. 2001
    ....
  30. ncbi request reprint Peptidase activity of Escherichia coli aminopeptidase A is not required for its role in Xer site-specific recombination
    R McCulloch
    Department of Genetics, University of Glasgow, UK
    Mol Microbiol 12:241-51. 1994
    ..Here we show that a mutation directed at the presumptive active site of PepA creates a protein with no detectable peptidase activity in vitro or in vivo, but which still functions normally in Xer site-specific recombination at cer...
  31. pmc Xer-mediated site-specific recombination at cer generates Holliday junctions in vivo
    R McCulloch
    University of Glasgow, Institute of Genetics, UK
    EMBO J 13:1844-55. 1994
    ..Controlled XerC expression is also used to analyse the efficiency of recombination between variant cer sites containing sequence alterations and heterologies within their central regions...
  32. ncbi request reprint Transformation of monomorphic and pleomorphic Trypanosoma brucei
    Richard McCulloch
    Welcome Centre for Molecular Parasitology, University of Glasgow, United Kingdom
    Methods Mol Biol 262:53-86. 2004
    ..We consider the application of transformation to recombination, as well as the uses of transforming the different life cycle stages and strain types...
  33. ncbi request reprint DNA metabolism and genetic diversity in Trypanosomes
    Carlos Renato Machado
    Department of Biochemistry and Immunology, Universidade Federal de Minas Gerais, Belo Horizonte, Brazil
    Mutat Res 612:40-57. 2006
    ..cruzi strains, and suggest that metabolic changes in the mismatch repair pathway could be an important source of antigenic diversity found within the T. cruzi population...
  34. doi request reprint Mismatch repair in Trypanosoma brucei: heterologous expression of MSH2 from Trypanosoma cruzi provides new insights into the response to oxidative damage
    Alice Machado-Silva
    Instituto de Ciencias Biologicas, Universidade Federal de Minas Gerais, Departamento de Bioquimica e Imunologia, Belo Horizonte, Brazil
    Gene 411:19-26. 2008
    ..brucei cells to H2O2, suggests an additional role of MSH2 in dealing with oxidative damage in these parasites, which may occur independently of MMR...
  35. pmc Sequence homology and microhomology dominate chromosomal double-strand break repair in African trypanosomes
    Lucy Glover
    London School of Hygiene and Tropical Medicine, Keppel Street, London, WC1E 7HT, UK
    Nucleic Acids Res 36:2608-18. 2008
    ..These DSBR pathways available to T. brucei likely underlie patterns of antigenic variation and the evolution of the vast VSG gene family...