DAVID SPRAY

Summary

Affiliation: Albert Einstein College of Medicine
Country: USA

Publications

  1. ncbi request reprint Prospects for rational development of pharmacological gap junction channel blockers
    David C Spray
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Curr Drug Targets 3:455-64. 2002
  2. pmc Modulatory effects of cAMP and PKC activation on gap junctional intercellular communication among thymic epithelial cells
    Oscar K Nihei
    Laboratory of Cellular Communication, Oswaldo Cruz Institute, The Oswaldo Cruz Foundation, Rio de Janeiro, Brazil
    BMC Cell Biol 11:3. 2010
  3. ncbi request reprint Functional connexin "hemichannels": a critical appraisal
    David C Spray
    Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Glia 54:758-73. 2006
  4. ncbi request reprint Hypertension in connexin40-null mice: a renin disorder
    D C Spray
    Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Kidney Int 72:781-2. 2007
  5. ncbi request reprint Organizational principles of the connexin-related brain transcriptome
    David C Spray
    Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine, 1410 Pelham Parkway South, Bronx, NY 10461, USA
    J Membr Biol 218:39-47. 2007
  6. ncbi request reprint Connexin43 null mice reveal that astrocytes express multiple connexins
    R Dermietzel
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Brain Res Brain Res Rev 32:45-56. 2000
  7. ncbi request reprint Transcriptomic characterization of four classes of cell-cell/cell-matrix genes in brains and hearts of wild type and connexin43 null mice
    D A Iacobas
    Rose F Kennedy Center, Albert Einstein College of Medicine, 1410 Pelham Parkway South, New York, NY 10461, USA
    Rom J Physiol 39:91-116. 2002
  8. ncbi request reprint [Transcription regulation and coordination of some cell signaling genes in brain and heart of connexin 43 null mouse]
    D A Iacobas
    Albert Einstein College of Medicine, New York, USA
    Rev Med Chir Soc Med Nat Iasi 107:534-9. 2003
  9. ncbi request reprint Gap junction expression and cell proliferation in differentiating cultures of Cx43 KO mouse hepatocytes
    T Kojima
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Am J Physiol Gastrointest Liver Physiol 281:G1004-13. 2001
  10. ncbi request reprint Structural changes in lenses of mice lacking the gap junction protein connexin43
    Y Gao
    Department of Neuroscience, Rose F Kennedy Center, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Invest Ophthalmol Vis Sci 39:1198-209. 1998

Research Grants

Detail Information

Publications94

  1. ncbi request reprint Prospects for rational development of pharmacological gap junction channel blockers
    David C Spray
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Curr Drug Targets 3:455-64. 2002
    ....
  2. pmc Modulatory effects of cAMP and PKC activation on gap junctional intercellular communication among thymic epithelial cells
    Oscar K Nihei
    Laboratory of Cellular Communication, Oswaldo Cruz Institute, The Oswaldo Cruz Foundation, Rio de Janeiro, Brazil
    BMC Cell Biol 11:3. 2010
    ..We investigated the effects of the signaling molecules, cyclic AMP (cAMP) and protein-kinase C (PKC), on gap junctional intercellular communication (GJIC) between thymic epithelial cells (TEC)...
  3. ncbi request reprint Functional connexin "hemichannels": a critical appraisal
    David C Spray
    Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Glia 54:758-73. 2006
    ..We advise hemichannel researchers to proceed with caution and an open mind...
  4. ncbi request reprint Hypertension in connexin40-null mice: a renin disorder
    D C Spray
    Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Kidney Int 72:781-2. 2007
    ..In mice lacking this gap junction protein, renin secretion is high and not regulated by arteriolar pressure...
  5. ncbi request reprint Organizational principles of the connexin-related brain transcriptome
    David C Spray
    Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine, 1410 Pelham Parkway South, Bronx, NY 10461, USA
    J Membr Biol 218:39-47. 2007
    ..These findings suggest several principles regarding regulatory transcriptomic networks involving gap junction genes and raise the issue of the underlying cause of connexin null phenotypes as well as mechanisms of regulation...
  6. ncbi request reprint Connexin43 null mice reveal that astrocytes express multiple connexins
    R Dermietzel
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Brain Res Brain Res Rev 32:45-56. 2000
    ....
  7. ncbi request reprint Transcriptomic characterization of four classes of cell-cell/cell-matrix genes in brains and hearts of wild type and connexin43 null mice
    D A Iacobas
    Rose F Kennedy Center, Albert Einstein College of Medicine, 1410 Pelham Parkway South, New York, NY 10461, USA
    Rom J Physiol 39:91-116. 2002
    ....
  8. ncbi request reprint [Transcription regulation and coordination of some cell signaling genes in brain and heart of connexin 43 null mouse]
    D A Iacobas
    Albert Einstein College of Medicine, New York, USA
    Rev Med Chir Soc Med Nat Iasi 107:534-9. 2003
    ....
  9. ncbi request reprint Gap junction expression and cell proliferation in differentiating cultures of Cx43 KO mouse hepatocytes
    T Kojima
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Am J Physiol Gastrointest Liver Physiol 281:G1004-13. 2001
    ....
  10. ncbi request reprint Structural changes in lenses of mice lacking the gap junction protein connexin43
    Y Gao
    Department of Neuroscience, Rose F Kennedy Center, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Invest Ophthalmol Vis Sci 39:1198-209. 1998
    ..To investigate the role of the gap junction protein connexin43 (Cx43), which is predominantly expressed in lens epithelial cells in the control of lens development and organization...
  11. ncbi request reprint Gap junction-mediated bidirectional signaling between human fetal hippocampal neurons and astrocytes
    R Rozental
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Dev Neurosci 23:420-31. 2001
    ....
  12. ncbi request reprint Control and variability of gene expression in mouse brain and in a neuroblastoma cell line
    D A Iacobas
    Albert Einstein College of Medicine, Department of Neuroscience, Molecular Biology Core, Rose F Kennedy Center, Room 915C, 1410 Pelham Parkway South, New York, NY 10461, USA
    Rom J Physiol 39:71-90. 2002
    ..In brains of different animals and in separate cultures of N2A cells, unique sets ofgenes exhibited exceptional stability or were highly variable. Possible implications for such tight or loose transcriptional control are discussed...
  13. pmc Quinine blocks specific gap junction channel subtypes
    M Srinivas
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Proc Natl Acad Sci U S A 98:10942-7. 2001
    ..Moreover, quinine derivatives that are excluded from other types of membrane channels may provide molecules with connexin-specific as well as connexin-selective blocking activity...
  14. pmc Human and mouse microglia express connexin36, and functional gap junctions are formed between rodent microglia and neurons
    K Dobrenis
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Neurosci Res 82:306-15. 2005
    ....
  15. ncbi request reprint Temporal expression of neuronal connexins during hippocampal ontogeny
    R Rozental
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Brain Res Brain Res Rev 32:57-71. 2000
    ..In addition, we describe the functional properties and expression pattern of the newly discovered neuronal-specific gap junctional protein, Cx36, in the developing mouse fetal hippocampus and in the rat retina and brain...
  16. ncbi request reprint Gap junctions in the nervous system
    R Rozental
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Brain Res Brain Res Rev 32:11-5. 2000
    ....
  17. ncbi request reprint Gap junctions: the "kiss of death" and the "kiss of life"
    A F Andrade-Rozental
    Department of Neuroscience, Albert Einstein College of Medicine, Montefiore Medical Center, Bronx, NY 10461, USA
    Brain Res Brain Res Rev 32:308-15. 2000
    ..In addition, potential applications are discussed for use of the "good Samaritan effect", a mechanism by which bystander cells have been suggested to prevent cytotoxicity...
  18. pmc Sex-dependent gene regulatory networks of the heart rhythm
    D A Iacobas
    Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine, Kennedy Center, New York, NY 10461, USA
    Funct Integr Genomics 10:73-86. 2010
    ....
  19. pmc P2X7 receptor-Pannexin1 complex: pharmacology and signaling
    R Iglesias
    The Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York, USA
    Am J Physiol Cell Physiol 295:C752-60. 2008
    ....
  20. ncbi request reprint Gap junctions in vascular tissues. Evaluating the role of intercellular communication in the modulation of vasomotor tone
    G J Christ
    Department of Urology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Circ Res 79:631-46. 1996
    ....
  21. pmc Intercellular communication in spinal cord astrocytes: fine tuning between gap junctions and P2 nucleotide receptors in calcium wave propagation
    E Scemes
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Neurosci 20:1435-45. 2000
    ..Intercellular communication via calcium waves therefore is sustained in Cx43 null mice by a finely tuned interaction between gap junction-dependent and independent mechanisms...
  22. ncbi request reprint Inhibition of endothelial cell migration, intercellular communication, and vascular tube formation by thromboxane A(2)
    A W Ashton
    Department of Medicine Cardiology, The Albert Einstein College of Medicine of Yeshiva University, Bronx, New York 10461, USA
    J Biol Chem 274:35562-70. 1999
    ..Thus, this finding that TXA(2) mimetics can prevent HEC migration and tube formation, possibly by impairing intercellular communication, suggests that antagonizing TXA(2) signaling might enhance vascularization of ischemic tissue...
  23. pmc Gap junction channels: distinct voltage-sensitive and -insensitive conductance states
    A P Moreno
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York 10461
    Biophys J 67:113-9. 1994
    ..These studies thus demonstrate that for certain gap junction channels, closure in response to specific stimuli may be graded, rather than all-or-none...
  24. pmc Molecular characterization and functional expression of the human cardiac gap junction channel
    G I Fishman
    Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461
    J Cell Biol 111:589-98. 1990
    ..These first measurements of the HCGJ channel are similar to the junctional conductance recorded between pairs of rat or guinea pig cardiocytes...
  25. ncbi request reprint Gap junctions remain open during cytochrome c-induced cell death: relationship of conductance to 'bystander' cell killing
    K Cusato
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Cell Death Differ 13:1707-14. 2006
    ....
  26. pmc Functional analysis of human cardiac gap junction channel mutants
    G I Fishman
    Department of Medicine, Albert Einstein College of Medicine, Bronx, NY 10461
    Proc Natl Acad Sci U S A 88:3525-9. 1991
    ..Furthermore, since the mutant connexins are missing several consensus phosphorylation sites, modification of these particular sites may not be required for membrane insertion or assembly of human connexin43 into functional channels...
  27. pmc Properties of gap junction channels formed by Cx46 alone and in combination with Cx50
    M G Hopperstad
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Biophys J 79:1954-66. 2000
    ..We conclude that different combinations of Cx46 and Cx50 within gap junction channels lead to unique biophysical properties...
  28. ncbi request reprint TPA induced expression and function of human connexin 26 by post-translational mechanisms in stably transfected neuroblastoma cells
    T Kojima
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, USA
    Cell Struct Funct 24:435-41. 1999
    ..These results suggest that induction of exogenous Cx26 in neuroblastoma cells by TPA treatment is controlled by post-translational mechanisms...
  29. ncbi request reprint Induction of tight junctions in human connexin 32 (hCx32)-transfected mouse hepatocytes: connexin 32 interacts with occludin
    T Kojima
    Department of Neuroscience, Albert Einstein College of Medicine, New York, NY, USA
    Biochem Biophys Res Commun 266:222-9. 1999
    ..These results indicate that in hepatocytes, gap junction, and tight junction expression are closely coordinated, and that Cx32 may play a role in regulating occludin expression...
  30. ncbi request reprint Cytokine regulation of neuronal differentiation of hippocampal progenitor cells
    M F Mehler
    Department of Neurology, Albert Einstein College of Medicine, Bronx, New York 10461
    Nature 362:62-5. 1993
    ..Further, they define an in vitro model system that may facilitate molecular analysis of graded stages of mammalian neuronal differentiation...
  31. ncbi request reprint Functional demonstration of connexin-protein binding using surface plasmon resonance
    H S Duffy
    Albert Einstein College of Medicine, Bronx, New York 10464, USA
    Cell Commun Adhes 8:225-9. 2001
    ..The combined application of proteomic and functional studies is expected to identify which proteins within heart tissue interact with Cx43 and what roles they may play in gap junction function...
  32. ncbi request reprint A novel casein kinase 2 alpha-subunit regulates membrane protein traffic in the human hepatoma cell line HuH-7
    X Shi
    Marion Bessin Liver Research Center, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 276:2075-82. 2001
    ..This study suggests that HuH-7 cells express at least three CK2alpha isoforms and that the pleiotropic TRF1 phenotype is a consequence of a reduction in total CK2 expression...
  33. ncbi request reprint Growth-suppressive function of human connexin32 in a conditional immortalized mouse hepatocyte cell line
    T Kojima
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    In Vitro Cell Dev Biol Anim 37:589-98. 2001
    ..Cx32 transfectants using a conditional immortalized mouse hepatocyte may be useful for examining the mechanisms of growth and differentiation in hepatocytes by gap junction expression...
  34. ncbi request reprint The "patholog" of the genes expression profile, a new tool in defining, evaluating, and classifying genetic diseases
    A D Iacobaş
    Albert Einstein College of Medicine, Bronx, NY, USA
    Rom J Physiol 37:59-67. 2000
    ..A publicly available database will be opened where every interested experimentalist could introduce his/her results and process them according to our procedure...
  35. ncbi request reprint New protocol in spotting microarray technique
    A D Iacobaş
    Albert Einstein College of Medicine, Bronx, NY, USA
    Rom J Physiol 37:69-80. 2000
    ..In this contribution, we propose and verify two procedures to correct the spot ratios and a new protocol to get the normal variability of the digital gene expression...
  36. pmc mRNA from NCB-20 cells encodes the N-methyl-D-aspartate/phencyclidine receptor: a Xenopus oocyte expression study
    J Lerma
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461
    Proc Natl Acad Sci U S A 86:1708-11. 1989
    ..Moreover, NCB-20 mRNA may be an appropriate starting material for cloning the cDNA(s) encoding the NMDA/PCP-receptor complex...
  37. ncbi request reprint Transcriptomic changes in developing kidney exposed to chronic hypoxia
    Dumitru A Iacobas
    Department of Neuroscience, Kennedy Center, Room 915C, Albert Einstein College of Medicine, 1410 Pelham Parkway South, Bronx, New York, NY 10461, USA
    Biochem Biophys Res Commun 349:329-38. 2006
    ..Transcriptional control was enhanced in CCH but not in CIH. Thus, CCH and CIH both alter gene expression and retard maturation with the more profound changes occurring in CCH than in CIH...
  38. ncbi request reprint Cardiac connexins: genes to nexus
    Heather S Duffy
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Adv Cardiol 42:1-17. 2006
    ..Binding sites may either correspond to structured regions within the connexin molecules or be unstructured, leading to presumably low-affinity and dynamic interactions...
  39. pmc Lack of "hemichannel" activity in insulin-producing cells
    Eliana Scemes
    Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York, USA
    Cell Commun Adhes 15:143-54. 2008
    ..Thus, insulin-producing cells are expected to provide a useful tool in the further characterization of hemichannel composition, properties, and physiological relevance...
  40. ncbi request reprint Microarray technology in the investigation of diseases of myocardium with special reference to infection
    Shankar Mukherjee
    Department of Pathology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Front Biosci 11:1802-13. 2006
    ..This paper provides an overview of microarray technology and its recent developments followed by its usage in studies of cardiovascular disease and how it pertains to viral and parasitic infections of the heart...
  41. pmc A stochastic two-dimensional model of intercellular Ca2+ wave spread in glia
    Dumitru A Iacobas
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York, USA
    Biophys J 90:24-41. 2006
    ..Such modeling is expected to be useful for testing phenomenological hypotheses and in understanding consequences of alteration of system components under experimental or pathological conditions...
  42. pmc Similar transcriptomic alterations in Cx43 knockdown and knockout astrocytes
    Dumitru A Iacobas
    Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Cell Commun Adhes 15:195-206. 2008
    ....
  43. ncbi request reprint A developmental switch in the expression of aquaporin-4 and Kir4.1 from horizontal to Müller cells in mouse retina
    Alejandra Bosco
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Invest Ophthalmol Vis Sci 46:3869-75. 2005
    ..This study was conducted to determine the presence of AQP4 and Kir4.1 proteins in the developing mouse retina...
  44. ncbi request reprint Blockade of gap junctions in vivo provides neuroprotection after perinatal global ischemia
    Mara H de Pina-Benabou
    Department of Cell Biology and Anatomy, New York Medical College, Valhalla, NY 10595, USA
    Stroke 36:2232-7. 2005
    ..We investigated the contribution of gap junctions to brain damage and delayed neuronal death produced by oxygen-glucose deprivation (OGD)...
  45. ncbi request reprint New possible roles for aquaporin-4 in astrocytes: cell cytoskeleton and functional relationship with connexin43
    Grazia P Nicchia
    Department of General and Environmental Physiology and Centre of Excellence in Comparative Genomics CEGBA, University of Bari, Bari, Italy
    FASEB J 19:1674-6. 2005
    ..Finally, the strong down-regulation of Cx43 and cell coupling in AQP4 KD mouse astrocytes indicate that a functional relationship likely exists between water channels and gap junctions in brain astrocytes...
  46. ncbi request reprint Connexin 26 expression prevents down-regulation of barrier and fence functions of tight junctions by Na+/K+-ATPase inhibitor ouabain in human airway epithelial cell line Calu-3
    Mitsuru Go
    Department of Pathology, Sapporo Medical University School of Medicine, S1 W17 Sapporo 060 8556, Japan
    Exp Cell Res 312:3847-56. 2006
    ..These results suggest that Cx26 expression, but not the mediated intercellular communication, may regulate tight junction barrier and fence functions in human airway epithelial cell line Calu-3...
  47. ncbi request reprint Block of specific gap junction channel subtypes by 2-aminoethoxydiphenyl borate (2-APB)
    Donglin Bai
    Department of Physiology and Pharmacology, University of Western Ontario, London, Ontario, Canada
    J Pharmacol Exp Ther 319:1452-8. 2006
    ..The differential efficacy of block by 2-APB of gap junction channels formed by different connexins may provide a useful tool that could be exploited in gap junction research to selectively block certain gap junction channel subtypes...
  48. pmc Gap junction remodeling and cardiac arrhythmogenesis in a murine model of oculodentodigital dysplasia
    Nellie Kalcheva
    Leon H Charney Division of Cardiology, New York University School of Medicine, New York, NY 10016, USA
    Proc Natl Acad Sci U S A 104:20512-6. 2007
    ..In summary, our data demonstrate that the I130T mutation interferes with Cx43 posttranslational processing, resulting in diminished cell-cell coupling, slowing of impulse propagation, and a proarrhythmic substrate...
  49. pmc Aquaporin-4 water channels in enteric neurons
    Mia M Thi
    Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York, USA
    J Neurosci Res 86:448-56. 2008
    ..It is known that submucosal neurons control transport processes in the intestinal mucosa, and the high percentage of aquaporin-4-positive submucosal neurons suggests that aquaporin-4 contributes to this function...
  50. pmc Alterations in myocardial gene expression associated with experimental Trypanosoma cruzi infection
    Shankar Mukherjee
    Department of Pathology, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Genomics 91:423-32. 2008
    ..These data may provide insight into the pathogenesis of chagasic cardiomyopathy and provide new targets for intervention...
  51. pmc pH-dependent dimerization of the carboxyl terminal domain of Cx43
    Paul L Sorgen
    Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, 68198, USA
    Biophys J 87:574-81. 2004
    ..Dimerization may alter the affinity of Cx43CT regions for specific molecular partners, thus modifying the regulation of gap junction channels...
  52. ncbi request reprint Gap junction and purinergic P2 receptor proteins as a functional unit: insights from transcriptomics
    Dumitru A Iacobas
    Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    J Membr Biol 217:83-91. 2007
    ..It is proposed that coordination analysis of gene expression may provide a novel unbiased strategy for the identification of proteins belonging to supramolecular complexes...
  53. ncbi request reprint Connexin-dependent transcellular transcriptomic networks in mouse brain
    Dumitru A Iacobas
    Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine, New York, NY, USA
    Prog Biophys Mol Biol 94:169-85. 2007
    ..The transcription factors also formed network hubs with genes from other functional categories, thus allowing regulation of one functional pathway through manipulation of another...
  54. ncbi request reprint Transfection of mammalian cells with connexins and measurement of voltage sensitivity of their gap junctions
    Cristiane Del Corsso
    The Dominick P Purpura Department of Neuroscience, Albert Einstein College of Medicine, 1410 Pelham Parkway South, Bronx, New York 10461, USA
    Nat Protoc 1:1799-809. 2006
    ..Electrophysiological protocols are presented for analysis of voltage dependence and single-channel conductance of gap junction channels as well as for studies of chemical gating of these channels...
  55. ncbi request reprint The gap junction protein connexin32 interacts with the Src homology 3/hook domain of discs large homolog 1
    Heather S Duffy
    Department of Pharmacology, Columbia University, New York, New York 10032, USA
    J Biol Chem 282:9789-96. 2007
    ..Together, these results suggest that loss of Cx32 alters the levels, localization, and interactions of the tumor suppressor protein Dlgh1, events known in other systems to alter cell cycle and increase tumorigenicity...
  56. pmc Pannexin1 is part of the pore forming unit of the P2X(7) receptor death complex
    Silviu Locovei
    Department of Physiology and Biophysics, University of Miami School of Medicine, 1600 NW 10th Ave, Miami, FL 33136, USA
    FEBS Lett 581:483-8. 2007
    ..Thus, pannexin1 appears to be the molecular substrate for the permeabilization pore (or death receptor channel) recruited into the P2X(7)R signaling complex...
  57. pmc Connexin43 and the brain transcriptome of newborn mice
    Dumitru A Iacobas
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Genomics 89:113-23. 2007
    ..This study indicates striking redundancy of expression controls over functional pathways and suggests that certain genes may play roles similar to or opposite that of Cx43 in organizing the brain transcriptome...
  58. ncbi request reprint The role of connexins in controlling cell growth and gene expression
    Elissavet Kardami
    Institute of Cardiovascular Sciences, University of Manitoba and St Boniface Research Centre, Winnipeg, Man, Canada
    Prog Biophys Mol Biol 94:245-64. 2007
    ..Finally, Section 4 will pay tribute to the rapidly emerging realization that connexins such as Cx43 and Cx32 exert important and extensive effects on gene expression, particularly those genes linked to growth regulation...
  59. ncbi request reprint Microarray analysis of changes in gene expression in a murine model of chronic chagasic cardiomyopathy
    Shankar Mukherjee
    Department of Pathology, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Parasitol Res 91:187-96. 2003
    ..The regulated genes are interpreted in the pathogenesis of chagasic heart disease...
  60. ncbi request reprint Sensitivity of the brain transcriptome to connexin ablation
    Dumitru A Iacobas
    Department of Neuroscience, Albert Einstein College of Medicine, 1410 Pelham Parkway South, Kennedy Center, Room 915C, New York, NY 10461, United States
    Biochim Biophys Acta 1711:183-96. 2005
    ..These findings lead to the hypothesis that connexin expression may represent a central node in the regulation of gene expression patterns in brain...
  61. ncbi request reprint Regulation of connexin43 protein complexes by intracellular acidification
    Heather S Duffy
    Department of Neuroscience, Albert Einstein College of Medicine, 1410 Pelham Pkwy S, Bronx, NY 10464, USA
    Circ Res 94:215-22. 2004
    ..These data suggest that protracted acidification may remodel protein-protein interactions involving Cx43 and thus provide an important protective mechanism to limit lesion spread after ischemic injury...
  62. pmc Following tracks of hemichannels
    Rolf Dermietzel
    Department of Neuroanatomy and Molecular Brain Research, Ruhr University Bochum, Bochum, Germany
    Cell Commun Adhes 10:335-40. 2003
    ..Pulse labeling with the peptide showed a decrease in the number of dual-labeled vesicles over time, indicating degradation and/or concurrent recycling of hemichannel vesicles...
  63. ncbi request reprint Kinetics of protein-protein interactions of connexins: use of enzyme linked sorbent assays
    Heather S Duffy
    Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Cell Commun Adhes 10:207-10. 2003
    ..These data confirm findings using Surface Plasmon Resonance (1) and indicate that ELSA can be a useful tool in determining the kinetics of protein-protein interactions...
  64. ncbi request reprint Molecular cloning and functional expression of zfCx52.6: a novel connexin with hemichannel-forming properties expressed in horizontal cells of the zebrafish retina
    Georg Zoidl
    Department of Neuroanatomy and Molecular Brain Research, Ruhr University Bochum, University Street 150, D 44801 Bochum, Germany
    J Biol Chem 279:2913-21. 2004
    ....
  65. pmc Array analysis of gene expression in connexin-43 null astrocytes
    Dumitru A Iacobas
    Department of Neuroscience, Albert Einstein College of Medicine, New York 10461, USA
    Physiol Genomics 15:177-90. 2003
    ..These data indicate a surprisingly high degree of impact of deletion of Cx43 on other astrocyte genes, implying that gap junction gene expression alters numerous processes in addition to intercellular communication...
  66. ncbi request reprint Connexin family members target to lipid raft domains and interact with caveolin-1
    Anne Lane Schubert
    Department of Molecular Pharmacology and Albert Einstein Cancer Center and Department of Neuroscience, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, New York 10461, USA
    Biochemistry 41:5754-64. 2002
    ..Our results provide the first evidence that connexins interact with caveolins and partition into lipid raft domains and indicate that these interactions are connexin specific...
  67. ncbi request reprint P2Y(1) receptor activation enhances the rate of rat pinealocyte-induced extracellular acidification via a calcium-dependent mechanism
    Zulma Silva Ferreira
    Department of Physiology, Instituto de Biociencias, Universidade de Sao Paulo, Sao Paulo, Brazil
    Pharmacology 69:33-7. 2003
    ..Clamping [Ca(2+)](i) with BAPTA (30 and 50 micromol/l) led to inhibition of ADP-induced increase in ECAR. Agonist and antagonist data indicate P2Y(1) activation leads to a [Ca(2+)](i)-dependent acidification of the extracellular medium...
  68. ncbi request reprint Gap junctions mediate bystander cell death in developing retina
    Karen Cusato
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Neurosci 23:6413-22. 2003
    ..This novel finding of naturally occurring bystander cell death may have important implications in the histogenesis and pathology of the nervous system...
  69. ncbi request reprint Closure of gap junction channels by arylaminobenzoates
    Miduturu Srinivas
    Department of Neuroscience, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Mol Pharmacol 63:1389-97. 2003
    ..We propose that FFA inhibits gap junctions by inducing a conformational change in the protein upon binding to a site that is presumably located within the membrane...
  70. pmc Acute downregulation of Cx43 alters P2Y receptor expression levels in mouse spinal cord astrocytes
    Sylvia O Suadicani
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York, USA
    Glia 42:160-71. 2003
    ..On the basis of these results, we propose that Cx43 regulates both modes of ICW by altering P2Y receptor subtype expression in addition to providing intercellular coupling...
  71. ncbi request reprint Formation of the gap junction nexus: binding partners for connexins
    Heather S Duffy
    Department of Neuroscience, Albert Einstein College of Medicine, 1410 Pelham Pkwy S, Bronx, NY 10461, USA
    J Physiol Paris 96:243-9. 2002
    ..This review summarizes current knowledge regarding the protein-protein interactions involving of connexin proteins and proposes hypothesized functions for these interactions...
  72. ncbi request reprint Fluid shear stress remodels expression and function of junctional proteins in cultured bone cells
    Mia M Thi
    New York Center for Biomedical Engineering, City College of the City University of New York, New York, 10031, USA
    Am J Physiol Cell Physiol 284:C389-403. 2003
    ..Such disconnection from the bone cell network may provide part of the signal whereby the disconnected cells or the remaining network initiate focal bone remodeling...
  73. ncbi request reprint pH-dependent intramolecular binding and structure involving Cx43 cytoplasmic domains
    Heather S Duffy
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 277:36706-14. 2002
    ..These data provide the first demonstration of chemically induced structural order and binding between cytoplasmic connexin domains...
  74. ncbi request reprint Cx32 formation and/or Cx32-mediated intercellular communication induces expression and function of tight junctions in hepatocytic cell line
    Takashi Kojima
    Department of Pathology, Sapporo Medical University School of Medicine, Sapporo 060 8556, Japan
    Exp Cell Res 276:40-51. 2002
    ..These results indicate that Cx32 formation and/or Cx32-mediated intercellular communication may participate in the formation of functional tight junctions and actin organization...
  75. ncbi request reprint Alterations of intercellular communication in neonatal cardiac myocytes from connexin43 null mice
    Monique J Vink
    Department of Neuroscience, Albert Einstein College of Medicine, 1300 Morris Park Avenue, Bronx, NY 10461, USA
    Cardiovasc Res 62:397-406. 2004
    ..To compare gap junction expression and intercellular coupling in wildtype neonatal cardiac myocytes to those from mice lacking the most abundant cardiac gap junction protein (connexin43, Cx43)...
  76. pmc Connexin43, the major gap junction protein of astrocytes, is down-regulated in inflamed white matter in an animal model of multiple sclerosis
    Elimor Brand-Schieber
    Department of Neurology, Albert Einstein College Medicine, Bronx, NY 10461, USA
    J Neurosci Res 80:798-808. 2005
    ..Our data support the notion that, in addition to damage of myelinating glia, altered astrocyte connectivity is a prominent feature of inflammatory demyelination...
  77. ncbi request reprint Illuminating gap junctions
    David C Spray
    Nat Methods 2:12-4. 2005
  78. ncbi request reprint Regulation of connexin43-protein binding in astrocytes in response to chemical ischemia/hypoxia
    Wei Li
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Biol Chem 280:7941-8. 2005
    ..These results identify new binding partners for connexin43 and demonstrate that interactions between connexin43 and protein kinases and phosphatases are dynamically altered as a consequence of connexin43 phosphorylation...
  79. ncbi request reprint Genes controlling multiple functional pathways are transcriptionally regulated in connexin43 null mouse heart
    Dumitru A Iacobas
    Department of Neuroscience Albert Einstein College of Medicine, Bronx, New York 10461, USA
    Physiol Genomics 20:211-23. 2005
    ..These findings reinforce the proposal that the cardiac abnormality in Cx43 null animals may be contributed by altered innervation and indicate that Cx43 deletion has consequences in addition to reduced intercellular communication...
  80. ncbi request reprint Structural changes in the carboxyl terminus of the gap junction protein connexin43 indicates signaling between binding domains for c-Src and zonula occludens-1
    Paul L Sorgen
    Department of Biochemistry and Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska 68198, USA
    J Biol Chem 279:54695-701. 2004
    ....
  81. ncbi request reprint Endotoxin unmasks the role of gap junctions in the liver
    Paulo R A V Correa
    Department of Medicine, Yale University School of Medicine, New Haven, CT, USA
    Biochem Biophys Res Commun 322:718-26. 2004
    ..The hepatic response to endotoxin is markedly impaired in the absence of Cx32. Thus, an important role of gap junctions in the liver is to assure integrated and uniform tissue response in times of stress...
  82. pmc Potent block of Cx36 and Cx50 gap junction channels by mefloquine
    Scott J Cruikshank
    Department of Neuroscience, Albert Einstein College of Medicine, Bronx, NY 10461, USA
    Proc Natl Acad Sci U S A 101:12364-9. 2004
    ..Thus, mefloquine is expected to be a useful tool to study the functional roles of Cx36 and Cx50...
  83. ncbi request reprint Modifications in the biophysical properties of connexin43 channels by a peptide of the cytoplasmic loop region
    Akiko Seki
    Department of Pharmacology, State University of New York Upstate Medical University, Syracuse 13210, USA
    Circ Res 95:e22-8. 2004
    ..Overall, we propose that the L2 acts as a "receptor" that interacts with a flexible intracellular gating element during channel gating. The full text of this article is available online at http://circres.ahajournals.org...
  84. ncbi request reprint Gene expression alterations in connexin null mice extend beyond the gap junction
    Dumitru A Iacobas
    Department of Neuroscience, Albert Einstein College of Medicine, Kennedy Center, Room 915C, 1300 Morris Park Ave, Bronx, NY 10461, USA
    Neurochem Int 45:243-50. 2004
    ..Here we discuss those results in the context of the strategies and data analysis paradigms that we are using in such studies...
  85. ncbi request reprint Molecular basis for pacemaker cells in epithelia
    M Fatima Leite
    Department of Physiology and Biophysics, Universidade Federal de Minas Gerais, 31270 901 Belo Horizonte, Brazil
    J Biol Chem 277:16313-23. 2002
    ..Together, these factors may allow epithelia to act in an integrated, organ-level fashion rather than as a collection of isolated cells...
  86. ncbi request reprint Mechanisms of glutamate release from astrocytes: gap junction "hemichannels", purinergic receptors and exocytotic release
    Vladimir Parpura
    Department of Cell Biology and Neuroscience, Center for Nanoscale Science and Engineering, University of California, Riverside, CA 92521, USA
    Neurochem Int 45:259-64. 2004
    ..In this review we limit our discussion to findings supporting the exocytotic release of glutamate, as well as two new pathways implicated in this release, the ionotropic (P2X) purinergic receptors and gap junction hemichannels...
  87. ncbi request reprint Characterization of connexin 30.3 and 43 in thymocytes
    Paula Candida Fonseca
    Laboratório de Pesquisas sobre o Timo, Departamento de Imunologia, Instituto Oswaldo Cruz, Fundacao Oswaldo Cruz, Brasil, Av Brasil, 4365 Manguinhos, 21045 900, Rio de Janeiro, RJ, Brazil
    Immunol Lett 94:65-75. 2004
    ..Concluding, in this study, we described for the first time the expression of connexins in thymocytes, which may constitute a new molecule having a functional role in thymocytes maturation...
  88. ncbi request reprint Sequence-specific resonance assignment of the carboxyl terminal domain of Connexin43
    Paul L Sorgen
    J Biomol NMR 23:245-6. 2002
  89. pmc Cognitive dysfunction in mice infected with Plasmodium berghei strain ANKA
    Mahalia S Desruisseaux
    Department of Pathology, Albert Einstein College of Medicine, Bronx, New York 10461, USA
    J Infect Dis 197:1621-7. 2008
    ..This murine model should facilitate the study of adjunctive methods to ameliorate adverse neurological outcomes in cerebral malaria...
  90. ncbi request reprint Connexins induce and maintain tight junctions in epithelial cells
    Takashi Kojima
    Department of Pathology, Sapporo Medical University School of Medicine, Sapporo, Japan
    J Membr Biol 217:13-9. 2007
    ..These results suggest that connexins can induce and maintain tight junctions in both GJIC-dependent and -independent manners in epithelial cells...
  91. ncbi request reprint Fluid shear stress upregulates vascular endothelial growth factor gene expression in osteoblasts
    Mia M Thi
    Department of Neuroscience, Albert Einstein College of Medicine of Yeshiva University, Bronx, New York, New York 10461, USA
    Ann N Y Acad Sci 1117:73-81. 2007
    ....
  92. pmc Trypanosoma cruzi induces changes in cardiac connexin43 expression
    Daniel Adesse
    Laboratorio de Ultra estrutura Celular, Instituto Oswaldo Cruz FIOCRUZ, Rio de Janeiro, Brazil
    Microbes Infect 10:21-8. 2008
    ..These data indicate that T. cruzi infection alters Cx43 expression both in vitro and in vivo. Disruptions in Cx43 may contribute to the pathogenesis of cardiac electrical alterations observed in T. cruzi infection...
  93. ncbi request reprint Flow cytometry analysis of gap junction-mediated cell-cell communication: advantages and pitfalls
    Paula Candida Fonseca
    Laboratório de Comunicação Celular, Departamento de Imunologia, Instituto Oswaldo Cruz, Fundação, Rio de Janeiro, Brasil
    Cytometry A 69:487-93. 2006
    ....
  94. ncbi request reprint Pathology of mechanical and gap junctional co-coupling at the intercalated disc: Is sepsis a junctionopathy?
    David C Spray
    Crit Care Med 35:2231-2. 2007

Research Grants5

  1. Luminescence Imaging: Molecules, Cells and Tissues
    DAVID SPRAY; Fiscal Year: 2006
    ..It also includes plans for training as well as inclusion of relevant methods in a graduate student course. [unreadable] [unreadable]..
  2. LENS GAP JUNCTIONS--IDENTITY AND PROPERTIES
    DAVID SPRAY; Fiscal Year: 2001
    ..abstract_text> ..
  3. Optimized Microarray Analysis of Neural Differentiation
    DAVID SPRAY; Fiscal Year: 2002
    ..We expect that these studies will not only improve our use of the microarray analysis, but will develop new concepts by which such expression patterns are generally used. ..
  4. GAP JUNCTIONS AND SCHWANN CELLS
    DAVID SPRAY; Fiscal Year: 2002
    ..Together, these studies should answer major questions regarding basic biology of Schwann cells and the role of gap junctions in physiological and pathophysiological conditions. ..
  5. Neuronal Gap Junctions: Cx36 Gating, Binding & Function
    DAVID SPRAY; Fiscal Year: 2006
    ..These studies use a multidisciplinary approach directed at exploring a new concept in the field and as such are expected to lead to novel understanding of regulation and roles of gap junctions in the nervous system. ..