K L Hartshorn

Summary

Affiliation: Boston University
Country: USA

Publications

  1. doi request reprint New look at an old problem: bacterial superinfection after influenza.
    Kevan L Hartshorn
    Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA
    Am J Pathol 176:536-9. 2010
  2. pmc Nrf2 protects human alveolar epithelial cells against injury induced by influenza A virus
    Beata Kosmider
    Department of Medicine, National Jewish Health, 1400 Jackson Street, Denver, CO 80206, USA
    Respir Res 13:43. 2012
  3. pmc Lack of the pattern recognition molecule mannose-binding lectin increases susceptibility to influenza A virus infection
    Wei Chuan Chang
    Department of Pediatrics, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    BMC Immunol 11:64. 2010
  4. ncbi request reprint Enhanced anti-influenza activity of a surfactant protein D and serum conglutinin fusion protein
    K L Hartshorn
    Departments of Medicine and Pathology, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    Am J Physiol Lung Cell Mol Physiol 278:L90-8. 2000
  5. pmc Salivary agglutinin and lung scavenger receptor cysteine-rich glycoprotein 340 have broad anti-influenza activities and interactions with surfactant protein D that vary according to donor source and sialylation
    Kevan L Hartshorn
    Department of Medicine, Section of Hematology Oncology, Boston University School of Medicine, Boston, MA 02118, USA
    Biochem J 393:545-53. 2006
  6. ncbi request reprint Innate defense against influenza A virus: activity of human neutrophil defensins and interactions of defensins with surfactant protein D
    Kevan L Hartshorn
    Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA
    J Immunol 176:6962-72. 2006
  7. pmc Reduced influenza viral neutralizing activity of natural human trimers of surfactant protein D
    Kevan L Hartshorn
    Boston University School of Medicine, Department of Medicine, Boston, MA, USA
    Respir Res 8:9. 2007
  8. ncbi request reprint Lung and salivary scavenger receptor glycoprotein-340 contribute to the host defense against influenza A viruses
    Kevan L Hartshorn
    Department of Medicine, Section of Hematology Oncology, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    Am J Physiol Lung Cell Mol Physiol 285:L1066-76. 2003
  9. pmc Contributions of the N- and C-terminal domains of surfactant protein d to the binding, aggregation, and phagocytic uptake of bacteria
    Kevan L Hartshorn
    Department of Medicine, Boston University School of Medicine, Boston, Massachussets 02118, USA
    Infect Immun 70:6129-39. 2002
  10. pmc Role of viral hemagglutinin glycosylation in anti-influenza activities of recombinant surfactant protein D
    Kevan L Hartshorn
    Department of Medicine, Boston University School of Medicine, Boston MA, USA
    Respir Res 9:65. 2008

Collaborators

Detail Information

Publications46

  1. doi request reprint New look at an old problem: bacterial superinfection after influenza.
    Kevan L Hartshorn
    Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA
    Am J Pathol 176:536-9. 2010
  2. pmc Nrf2 protects human alveolar epithelial cells against injury induced by influenza A virus
    Beata Kosmider
    Department of Medicine, National Jewish Health, 1400 Jackson Street, Denver, CO 80206, USA
    Respir Res 13:43. 2012
    ..Recent studies have shown the importance of lung antioxidant defense systems against injury by IAV. Nuclear factor-erythroid 2 related factor 2 (Nrf2) activates the majority of antioxidant genes...
  3. pmc Lack of the pattern recognition molecule mannose-binding lectin increases susceptibility to influenza A virus infection
    Wei Chuan Chang
    Department of Pediatrics, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA
    BMC Immunol 11:64. 2010
    ..These clinical observations were confirmed by animal model studies, in which mice genetically lacking MBL were susceptible to certain pathogens, including herpes simplex virus 2...
  4. ncbi request reprint Enhanced anti-influenza activity of a surfactant protein D and serum conglutinin fusion protein
    K L Hartshorn
    Departments of Medicine and Pathology, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    Am J Physiol Lung Cell Mol Physiol 278:L90-8. 2000
    ....
  5. pmc Salivary agglutinin and lung scavenger receptor cysteine-rich glycoprotein 340 have broad anti-influenza activities and interactions with surfactant protein D that vary according to donor source and sialylation
    Kevan L Hartshorn
    Department of Medicine, Section of Hematology Oncology, Boston University School of Medicine, Boston, MA 02118, USA
    Biochem J 393:545-53. 2006
    ..These findings may relate to inter-individual variations in innate defence against highly pathogenic IAV and to effects of aspiration of oral contents on SP-D-mediated lung functions...
  6. ncbi request reprint Innate defense against influenza A virus: activity of human neutrophil defensins and interactions of defensins with surfactant protein D
    Kevan L Hartshorn
    Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA
    J Immunol 176:6962-72. 2006
    ..These results are relevant to the early phase of host defense against IAV, and suggest a complex interplay between SP-D and HNPs at sites of active inflammation...
  7. pmc Reduced influenza viral neutralizing activity of natural human trimers of surfactant protein D
    Kevan L Hartshorn
    Boston University School of Medicine, Department of Medicine, Boston, MA, USA
    Respir Res 8:9. 2007
    ..We recently reported that the Thr/Thr 11 form of SP-D is associated with low serum levels and assembles predominantly as trimers as opposed to the more common multimeric forms of SP-D...
  8. ncbi request reprint Lung and salivary scavenger receptor glycoprotein-340 contribute to the host defense against influenza A viruses
    Kevan L Hartshorn
    Department of Medicine, Section of Hematology Oncology, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    Am J Physiol Lung Cell Mol Physiol 285:L1066-76. 2003
    ..Furthermore, removal of gp-340 from BAL fluid and saliva significantly reduced anti-IAV activity. Hence, gp-340 contributes to defense against IAV and may be particularly relevant to defense against SP-D-resistant viral strains...
  9. pmc Contributions of the N- and C-terminal domains of surfactant protein d to the binding, aggregation, and phagocytic uptake of bacteria
    Kevan L Hartshorn
    Department of Medicine, Boston University School of Medicine, Boston, Massachussets 02118, USA
    Infect Immun 70:6129-39. 2002
    ....
  10. pmc Role of viral hemagglutinin glycosylation in anti-influenza activities of recombinant surfactant protein D
    Kevan L Hartshorn
    Department of Medicine, Boston University School of Medicine, Boston MA, USA
    Respir Res 9:65. 2008
    ..Surfactant protein D (SP-D) plays an important role in innate defense against influenza A viruses (IAVs) and other pathogens...
  11. pmc Viral aggregating and opsonizing activity in collectin trimers
    Kevan L Hartshorn
    Boston University School of Medicine, EBRC 414, 650 Albany St, Boston, MA 02118, USA
    Am J Physiol Lung Cell Mol Physiol 298:L79-88. 2010
    ..This may involve increased affinity of individual CRDs for glycoconjugates displayed on host cells or the viral envelope...
  12. pmc Distinctive anti-influenza properties of recombinant collectin 43
    Kevan L Hartshorn
    Department of Medicine, Boston University School of Medicine, EBRC 414, 650 Albany Street, Boston, MA 02118, U S A
    Biochem J 366:87-96. 2002
    ..These findings could facilitate the development of recombinant collectins with novel antimicrobial properties...
  13. ncbi request reprint Role of surfactant protein A and D (SP-A and SP-D) in human antiviral host defense
    Kevan L Hartshorn
    Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA
    Front Biosci (Schol Ed) 2:527-46. 2010
    ..The relative importance of antiviral vs anti-inflammatory effects of SP-A and SP-D in viral infections and the potential use of these collectins as therapeutics for viral infections are under investigation...
  14. pmc Increasing antiviral activity of surfactant protein d trimers by introducing residues from bovine serum collectins: dissociation of mannan-binding and antiviral activity
    K L Hartshorn
    Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA
    Scand J Immunol 72:22-30. 2010
    ..Of interest, we show conservation of some monoclonal antibody-binding epitopes between bovine collectin NCRD and hSP-D, suggesting shared structural motifs...
  15. pmc Monoclonal antibody-assisted structure-function analysis of the carbohydrate recognition domain of surfactant protein D
    Kevan L Hartshorn
    Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    Am J Physiol Lung Cell Mol Physiol 299:L384-92. 2010
    ....
  16. pmc Mechanism of binding of surfactant protein D to influenza A viruses: importance of binding to haemagglutinin to antiviral activity
    K L Hartshorn
    Boston University School of Medicine, EBRC 414, 650 Albany Street, Boston, MA 02118, USA
    Biochem J 351:449-58. 2000
    ..These studies clarify the mechanisms of viral neutralization by collectins and might be useful in engineering collectins for enhanced antiviral activity...
  17. ncbi request reprint Influenza A virus up-regulates neutrophil adhesion molecules and adhesion to biological surfaces
    K L Hartshorn
    Department of Medicine, Boston University School of Medicine, Massachusetts 02118, USA
    J Leukoc Biol 65:614-22. 1999
    ..Up-regulation of adhesion molecules and adhesion are important, previously unrecognized, features of neutrophil activation by IAV. Further studies will be needed to clarify the mechanism of these effects...
  18. ncbi request reprint Bovine conglutinin gene exon structure reveals its evolutionary relationship to surfactant protein-D
    L S Liou
    Department of Medicine, Boston University School of Medicine, Boston City Hospital, MA 02118
    J Immunol 153:173-80. 1994
    ..On the basis of identical collagen domain structures, we suggest that conglutinin and bovine surfactant protein-D evolved from a gene duplication event occurring in Bovidae after divergence from other mammals...
  19. pmc Multiple components contribute to ability of saliva to inhibit influenza viruses
    M R White
    Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA
    Oral Microbiol Immunol 24:18-24. 2009
    ..Saliva is a potentially important barrier against respiratory viral infection but its mechanism of action is not well studied...
  20. ncbi request reprint Cooperative anti-influenza activities of respiratory innate immune proteins and neuraminidase inhibitor
    Mitchell R White
    Department of Medicine, Section of Hematology Oncology, Boston University, School of Medicine, Boston, MA 02118, USA
    Am J Physiol Lung Cell Mol Physiol 288:L831-40. 2005
    ..Hence, oseltamivir collaborates with innate immune proteins in inhibiting the initial infection of epithelial cells...
  21. ncbi request reprint Respiratory innate immune proteins differentially modulate the neutrophil respiratory burst response to influenza A virus
    Mitchell R White
    Dept of Medicine, Boston University School of Medicine, MA 02118, USA
    Am J Physiol Lung Cell Mol Physiol 289:L606-16. 2005
    ..As a result, the net effect of bronchoalveolar lavage fluids is to increase neutrophil uptake of IAV while reducing the respiratory burst response to virus...
  22. ncbi request reprint Increased antiviral and opsonic activity of a highly multimerized collectin chimera
    M R White
    Department of Medicine, Boston University School of Medicine, 80 East Concord Street, Boston, MA 02118, USA
    Biochem Biophys Res Commun 286:206-13. 2001
    ..These studies may have implications for development of collectins as therapeutic agents and understanding of natural variations in susceptibility to IAV infection...
  23. ncbi request reprint Inhibition of influenza viral neuraminidase activity by collectins
    T Tecle
    Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA
    Arch Virol 152:1731-42. 2007
    ..The markedly greater NA inhibitory activity of SP-D compared to SP-A may partly account for the finding that deletion of the SP-D gene in mice has a greater effect on viral replication in vivo...
  24. pmc Enhancement of antiviral activity of collectin trimers through cross-linking and mutagenesis of the carbohydrate recognition domain
    Mitchell R White
    Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA
    J Innate Immun 2:267-79. 2010
    ..These findings may be relevant for antiviral therapy...
  25. doi request reprint Critical role for cross-linking of trimeric lectin domains of surfactant protein D in antiviral activity against influenza A virus
    Tesfaldet Tecle
    Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA
    Biochem J 412:323-9. 2008
    ..These results show that antiviral activities of SP-D can be reproduced without the N-terminal and collagen domains and that cross-linking of NCRDs is essential for antiviral activity of SP-D with respect to IAV...
  26. ncbi request reprint Bovine conglutinin (BC) mRNA expressed in liver: cloning and characterization of the BC cDNA reveals strong homology to surfactant protein-D
    L S Liou
    Department of Medicine, Boston University School of Medicine, Boston City Hospital, MA 02118
    Gene 141:277-81. 1994
    ..Of interest, the expression of the BC mRNA, as determined by RNase protection assay, is restricted to liver, unlike bovine SP-D, a lung-surfactant protein...
  27. ncbi request reprint Influenza a viruses upregulate neutrophil toll-like receptor 2 expression and function
    R M Lee
    Department of Medicine, Section of Hematology Oncology, Boston University School of Medicine, Boston, MA 02118, USA
    Scand J Immunol 63:81-9. 2006
    ..These results indicate that IAV increases neutrophil surface expression of TLR2 and modulates functional responses to ligands that bind TLR2. These findings may clarify IAV-induced perturbation of neutrophil functions in vivo...
  28. ncbi request reprint Impact of neutrophils on antiviral activity of human bronchoalveolar lavage fluid
    Mitchell R White
    Department of Medicine, Boston University School of Medicine, Boston, Massachusetts 02118, USA
    Am J Physiol Lung Cell Mol Physiol 293:L1293-9. 2007
    ..g., as in chronic smoking or cystic fibrosis) may reduce SP-D levels and predispose to IAV infection. In contrast, acute inflammation, as occurs in the early phase of IAV infection, may promote neutrophil-mediated viral clearance...
  29. doi request reprint Review: Structural determinants of pattern recognition by lung collectins
    Barbara A Seaton
    Department of Physiology and Biophysics, Boston University School of Medicine, Massachusetts, USA
    Innate Immun 16:143-50. 2010
    ..Current research suggests that structural biology approaches will help to elucidate the molecular basis of pulmonary collectin-ligand recognition and facilitate development of new therapeutics based upon SP-A and SP-D...
  30. doi request reprint Interactions of alpha-, beta-, and theta-defensins with influenza A virus and surfactant protein D
    Mona Doss
    Department of Medicine, Boston University School of Medicine, Boston, MA 02118, USA
    J Immunol 182:7878-87. 2009
    ..Given their strong neutralizing activity and compatibility with SP-D, RCs may provide attractive prototypes for designing therapeutics that can prevent or treat respiratory infections caused by IAV...
  31. ncbi request reprint Neutrophil survival is markedly reduced by incubation with influenza virus and Streptococcus pneumoniae: role of respiratory burst
    G Engelich
    Department of Medicine and Pathology, Boston University School of Medicine, Massachusetts 02218, USA
    J Leukoc Biol 69:50-6. 2001
    ..pneumoniae. These findings may help to explain the increased susceptibility of IAV-infected patients to infections with S. pneumoniae...
  32. ncbi request reprint Surfactant protein D binds to human immunodeficiency virus (HIV) envelope protein gp120 and inhibits HIV replication
    Joseph Meschi
    Department of Medicine, Sections of Hematology Oncology, Boston University School of Medicine, EBRC 414, 650 Albany Street, Boston, MA 02118, USA
    J Gen Virol 86:3097-107. 2005
    ..SP-D is present in lung and airway fluids, as well as in blood and various mucosal locations, and could, like MBL, play a role in restricting HIV transmission or replication in vivo...
  33. ncbi request reprint Prophylaxis and treatment of influenza virus infection
    R Kandel
    Hebrew Rehabilitation Center for Aged, Harvard University School of Medicine, Boston, Massachusetts 02131-1097, USA
    BioDrugs 15:303-23. 2001
    ..Further advances in vaccination and antiviral therapy of influenza should remain a high priority...
  34. ncbi request reprint Acquired disorders of phagocyte function complicating medical and surgical illnesses
    G Engelich
    Section of Hematology Oncology and Department of Medicine, Boston University School of Medicine, Boston, MA, 02118, USA
    Clin Infect Dis 33:2040-8. 2001
    ..Given the advent of improved assays of phagocyte functions and treatments that can enhance these functions, there is a pressing need for more prospective studies of acquired phagocyte dysfunction...
  35. ncbi request reprint Human neutrophil defensins increase neutrophil uptake of influenza A virus and bacteria and modify virus-induced respiratory burst responses
    Tesfaldet Tecle
    Department of Medicine, Boston University School of Medicine, 650 Albany Street, Boston, MA 02118, USA
    J Immunol 178:8046-52. 2007
    ....
  36. doi request reprint Review: Defensins and cathelicidins in lung immunity
    Tesfaldet Tecle
    Department of Medicine, Boston University School of Medicine, Massachusetts, USA
    Innate Immun 16:151-9. 2010
    ....
  37. ncbi request reprint Novel strategies for prevention and treatment of influenza
    Ruth Kandel
    Harvard University School of Medicine, Hebrew Rehabilitation Center for Aged Internal Medicine Geriatrics, 1200 Centre Street, Boston, MA 02131 1097, USA
    Expert Opin Ther Targets 9:1-22. 2005
    ..Some adverse sequelae of influenza appear to relate to impairment or pathogenic activation of immune responses. Exciting recent findings in this area, with relevance to influenza treatment, are reviewed...
  38. ncbi request reprint Role of the respiratory burst in co-operative reduction in neutrophil survival by influenza A virus and Escherichia coli
    Georg Engelich
    Department of Medicine and Pathology, Boston University School of Medicine, MA 02218, USA
    J Med Microbiol 51:484-90. 2002
    ..coli, and reduced survival of these cells. These results indicate that co-operative induction of intracellular respiratory burst responses by IAV and E.coli mediates the reduced neutrophil survival caused by these pathogens in vitro...
  39. pmc Differentiated human alveolar type II cells secrete antiviral IL-29 (IFN-lambda 1) in response to influenza A infection
    Jieru Wang
    Department of Medicine, National Jewish Medical and Research Center, Denver, CO 80206, USA
    J Immunol 182:1296-304. 2009
    ..These results suggest that IL-29 exerts IFN-beta-independent protection in type II cells through direct activation of antiviral genes during IAV infection...
  40. doi request reprint Human defensins and LL-37 in mucosal immunity
    Mona Doss
    Boston University School of Medicine, Department of Medicine, Boston, Massachusetts, USA
    J Leukoc Biol 87:79-92. 2010
    ..g., Crohn's disease, atopic dermatitis, lung or urinary infections). Challenges for these studies are the redundancy of innate defense mechanisms and the presence and interactions of many innate defense proteins in mucosal secretions...
  41. ncbi request reprint Complementation of pulmonary abnormalities in SP-D(-/-) mice with an SP-D/conglutinin fusion protein
    Liqian Zhang
    Division of Pulmonary Biology, Cincinnati Children s Hospital Medical Center, Cincinnati, Ohio 45229 3039, USA
    J Biol Chem 277:22453-9. 2002
    ..Our findings also strongly suggest that the molecular mechanisms underlying impaired pulmonary host defense and abnormal lipid metabolism are distinct from those that promote ongoing inflammation and the development of emphysema...
  42. ncbi request reprint Effective targeting of pathogens to neutrophils via chimeric surfactant protein D/anti-CD89 protein
    Paul J Tacken
    Department of Biochemistry and Cell Biology, Graduate School of Animal Health, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
    J Immunol 172:4934-40. 2004
    ..In conclusion, rfSP-D/anti-CD89 effectively targeted three structurally unrelated pathogens to neutrophils. (Col)lectin-based chimeric proteins may thus offer promise for therapy of infectious disease...
  43. ncbi request reprint Porcine surfactant protein D is N-glycosylated in its carbohydrate recognition domain and is assembled into differently charged oligomers
    Martin van Eijk
    Department of Biochemistry and Cell Biology, Graduate School of Animal Health, Faculty of Veterinary Medicine, Utrecht University, The Netherlands
    Am J Respir Cell Mol Biol 26:739-47. 2002
    ..The removal of the carbohydrate moiety reduces the inhibitory effect of porcine SP-D on influenza A virus haemagglutination. Therefore, the carbohydrate moiety may influence interactions with pathogens...
  44. ncbi request reprint Interactions of influenza A virus with sialic acids present on porcine surfactant protein D
    Martin van Eijk
    Department of Biochemistry and Cell Biology, Graduate School of Animal Health, Faculty of Veterinary Medicine, Utrecht University, P O Box 80 176, 3508 TD Utrecht, The Netherlands
    Am J Respir Cell Mol Biol 30:871-9. 2004
    ....
  45. ncbi request reprint Porcine pulmonary collectins show distinct interactions with influenza A viruses: role of the N-linked oligosaccharides in the carbohydrate recognition domain
    Martin van Eijk
    Departments of Biochemistry and Cell Biology, Graduate School of Animal Health, Faculty of Veterinary Medicine, Utrecht University, Utrecht, The Netherlands
    J Immunol 171:1431-40. 2003
    ..A more efficient lung collectin-mediated immune response against IAV in pigs may play a role in providing conditions by which pigs can act as "mixing vessel" hosts that can lead to the production of reassortant, pandemic strains of IAV...